JP3536111B2 - Mucopolysaccharide fragmentation inhibitor and cosmetic - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a mucopolysaccharide fragmentation inhibitor, an active oxygen scavenger, an antioxidant, and a cosmetic containing these as active ingredients. In particular, the cosmetics according to the present invention are mainly intended to provide anti-aging cosmetics for skin and the like, but the field of use of the present invention is not limited to the technical field of the cosmetics, It is also widely applicable to various technical fields such as food and the like.

[0002]

2. Description of the Related Art Humans use oxygen as energy. As long as oxygen is used as energy, some oxygen becomes active oxygen such as hydrogen peroxide (H ₂ O ₂ ), superoxide (O ₂ ⁻ ), hydroxy radical (OH ⁻ ), etc. It has been proposed that it may cause aging and diseases of. And, in order to remove the harmful effect of active oxygen, superoxide dimethase (SO
Active oxygen can be directly eliminated by enzymes such as D).

By the way, since human skin is located in the outermost layer of the living body, it is constantly exposed to oxidative stress due to endogenous active oxygen or exogenous active oxygen generated by ultraviolet rays. The mucopolysaccharide represented by hyaluronic acid, which is the main component in the dermis of the skin, plays an important role in maintaining the firmness of the skin. Mucopolysaccharides are said to be fragmented by active oxygen and ultraviolet rays and lead to skin aging. Therefore, suppressing the fragmentation of mucopolysaccharides present in the dermis of the skin is important for skin aging.

Furthermore, the sebum secreted on the skin is useful for protecting the skin from exogenous stress, and this sebum is oxidized by ultraviolet rays to become lipid peroxide, and this lipid peroxide is applied to the skin. In addition to being a stimulus, it is said to be involved in aging by attacking cells to cause damage and causing various other adverse effects. Therefore, it is considered that suppressing the production of lipid peroxide is effective not only for preventing the deterioration of the skin (skin) condition but also for preventing the aging of the living body itself.

[0005]

DISCLOSURE OF THE INVENTION The present invention is to fragment mucopolysaccharides, which are the main constituents of the skin, in order to search for those having an action of suppressing skin aging from a group of extracts of various plants. Searching for something with an inhibitory effect,
Searching for substances that have an active oxygen (direct) scavenging effect that causes adverse effects such as aging, substances that are indirectly generated by the presence of active oxygen (such as lipid peroxide)
As a result of research for the purpose of searching for those that prevent the adverse effects such as aging, those having a mucopolysaccharide fragmentation-inhibiting action / effect from the above,
The present invention was completed based on the new finding of those having a more active oxygen scavenging effect and those having a more antioxidant effect.

Therefore, the present invention provides a stable and safe mucopolysaccharide fragmentation inhibitor, an active oxygen scavenger, an antioxidant, and an antioxidant which are free from side effects obtained from a group of extracts from natural products (plants). Cosmetics using these (antiaging cosmetics)
With the object of providing each of the active ingredients of the mucopolysaccharide fragmentation inhibitor, the active oxygen scavenger and the antioxidant according to the present invention, not only the technical field of cosmetics but also the technical field of medicine and foods. It also opens up a wide range of applications in the technical field of.

[0007]

[Means for Solving the Problems] In order to achieve the above-mentioned object, the present invention provides: Ryutan, Japanese bellflower, hibiscus, aloe, rhubarb, yellow sperm, Japanese lacquer, prolonging herb,
Sanshishi, Yangmei, Kakkon, Psycho, Senkyu, Hakaba, Bukuryo, Licorice, Peonies, Yokuinin, Spicy, Midsummer, Toki, Cinnamon, Huanglian, Peony skin, Gentiana, Baxa, Quintuple, Senburi, Gennoshoko, Mao, Yellow oak, Oogon, Chen skin, Turmeric, Goshuyu, Sage, Oregano, Rosemary, Laurel, Taragon, Onion,
Water or lower alcohol than each plant consisting of nutmeg, clove, wasabi, sabori, basil, chilli, bean tea, black tea, green tea, persimmon leaf, coffee, sugi, bee, wormwood, kumasasa, wolfberry, yab cypress, shiitake, or Various plant extracts extracted with lower alcohol aqueous solution, and L-cysteine, glutathione, mannitol, gallic acid, potassium sorbate, acetylacetone, triethanolamine, tannic acid, 4-t-butyl-4'-methoxy-dibenzo It is characterized by containing, as an active ingredient, one kind or two or more kinds selected from any one group or both groups of the compound group consisting of methane and 2-hydroxy-4-methylbenzophenone-5-sulfate. It constitutes a mucopolysaccharide fragmentation inhibitor.

Hibiscus, aloe, rhubarb, yellow sperm, arbutus, life-prolonging grass, sardine, Yangmei bark, kudzu root, psycho, senkyu, sojutsu, thin leaf, bukuro,
Licorice, peony, spicy, half-summer, toki, cinnamon, ten drugs, yellow lichen, peony skin, gentian, quintuplet, senburi, genosho, mao, yellow oak, apricot kernel, dried ginger, turmeric, eugon, hen skin, turmeric, ninfu, Kyoujitsu, ground yellow, garlic, sage, oregano, rosemary, laurel, celery, thyme, tarragon, onion, nut meg, mace, clove, wasabi, sabori, basil, pepper, bean tea, tea, green tea, persimmon leaf, coffee, Too bad, bee,
Wormwood, bear squirrel, wolfberry, yab cycad, shiitake mushroom, ginkgo, hijiki, squid, konbu, storm, sea turtle,
Group of various plant extracts extracted with water or a lower alcohol or a lower alcohol aqueous solution from each plant consisting of, and a group of compounds consisting of tannic acid, 2-hydroxy-4-methylbenzophenone-5-sulfate Or 1 or 2 selected from both groups
An active oxygen scavenger is characterized by containing at least one species as an active ingredient.

Hibiscus, aloe, rhubarb, yellow sperm, vomitula, life-prolonging grass, Yangmei peel, kudzu root, senkyu,
Soujutsu, thin leaf, licorice, peony, Yokuinin,
Chinese cabbage, cinnamon, ten medicines, yellow ream, peony skin, gentian, quintet, senburi, genosho, yellow oak, ginger, sardine, boar, garlic, sage, oregano, rosemary, laurel, celery, thyme, tarragon, nut meg, Mace, cloves, wasabi, sabori, basil, chili, bean tea, black tea, green tea, persimmon leaves, coffee, sugi, bee, wormwood, army chazzle, kumasasa, wolfberry, shiitake mushroom, hijiki, wakame, igusu, konbu , One or more selected from various plant extract groups extracted with water or a lower alcohol or a lower alcohol aqueous solution from each plant consisting of, Japanese porridge, sea turtle, blue seaweed, and the like, as an active ingredient. It constitutes an antioxidant.

It contains, as an active ingredient, one or more selected from the group consisting of the mucopolysaccharide fragmentation inhibitor described above, the active oxygen scavenger described above and the antioxidant described above. The cosmetics are characterized by that. By doing so, each invention is configured. Next, each invention will be described in detail.

The "mucopolysaccharide fragmentation inhibitor", "active oxygen scavenger", "antioxidant" and "cosmetics" according to the present invention are either one or both of various plant extracts and various compounds. It contains one or more selected from the above as an active ingredient. The various extracts of this plant refer to extracts extracted from various plants with water, lower alcohol or lower alcohol aqueous solution. The "lower alcohol" in this specification means methanol, ethanol, and propanol, and alcohols that can be mixed with water at an arbitrary ratio.

The test plants used in the examples of this specification were dried and powdered products of each plant. Further, the extract with lower alcohol of the plant in the examples of this specification was obtained by using a Soxhlet extractor. As the extract with water or a lower alcohol aqueous solution, each extract was obtained by leaching and extracting a predetermined amount of a sample under a predetermined condition with a predetermined amount of water or a lower alcohol aqueous solution. Then, various plant extracts are prepared by distilling off the organic solvent for the lower alcohol extract, lyophilizing the water extract as it is, and further lowering the lower alcohol of the extract for the lower alcohol extract. The fractions were concentrated under reduced pressure and then freeze-dried to obtain each plant extract.

First, the "mucopolysaccharide fragmentation inhibitor" according to the present invention will be described. The "mucopolysaccharide" referred to in the present invention refers to a concept including all mucopolysaccharides of neutral mucopolysaccharides and acidic mucopolysaccharides, and particularly mucopolysaccharides related to humans, that is, hyaluronic acid, chondroitin, and chondroitin 4-sulfate. Needless to say, chondroitin 6-sulfate, dermatan sulfate, heparan sulfate, pepperin and keratan sulfate I and keratan sulfate II are included.

The "mucopolysaccharide fragmentation inhibitor" according to the present invention has industrial applicability from the following viewpoints. That is, mucopolysaccharides (hyaluronic acid, etc.), which are the main constituents in the dermis of human skin, are considered to be important components for maintaining firmness of the skin. It is considered that this mucopolysaccharide loses its original function by being fragmented into a low molecular weight by ultraviolet rays and active oxygen generated in the living body. Therefore, preventing fragmentation of this mucopolysaccharide (such as hyaluronic acid) is considered to be effective in preventing wrinkles on human skin (skin) and preventing skin aging. Furthermore,
The "mucopolysaccharide fragmentation inhibitor" according to the present invention opens up a way of application in the industrial fields of medicine and food.

Mixture of various active ingredients (one or more selected from one or both of various plant extracts and various compounds) in the "mucopolysaccharide fragmentation inhibitor" according to the present invention. The amount (content) is not particularly limited, as it can be varied depending on the kind of the active ingredient and the combination thereof, the purpose of use, the embodiment, the form of use, the number of times of use and the like. In principle, an effective amount should be present, but generally 0.0001 to 100% by weight, preferably 1 to 10% by weight based on the composition of the mucopolysaccharide fragmentation inhibitor is used. it can. Furthermore, the active ingredient (various extracts of plants and various compounds) according to the present invention can exert the action and effect even with one kind, but it is excellent by appropriately combining two or more active ingredients. A synergistic effect can be produced. Naturally, the active ingredient according to the present invention (one or more selected from one or both of various plant extracts and various compounds) is used in combination with a known “mucopolysaccharide fragmentation inhibitor”. By doing so, an excellent synergistic effect can be achieved.

The mucopolysaccharide fragmentation-inhibiting action of various plant extracts and various compounds according to the present invention was measured by a known method. That is, by combining the fragmentation reaction by active oxygen (ascorbic acid-iron system) and the complex formation reaction between albumin and mucopolysaccharide, the fragmentation reaction of mucopolysaccharide by ultraviolet rays (UV-A) and albumin By combining the complex formation reaction with mucopolysaccharides, the measurement was performed and the presence or absence of each active ingredient was searched. Table 1 shows the measurement results of the water extract of each plant, Table 2 shows the measurement results of the lower alcohol aqueous solution (50% ethanol aqueous solution) extract, and Table 3
Indicates the measurement results of the lower alcohol (99.9% ethanol) extract, respectively.

The inventors of the present invention have identified the “mucopolysaccharide fragmentation inhibitor” according to the present invention with respect to plant extracts or compounds having a fragmentation inhibition rate of 20% or more in Table 1, Table 2 and Table 3 above. It was determined to be a plant extract and compound that can be used as That is, as the plant extract group, Ryutan, Kyokyo, Hibiscus, Aloe, Rhinoceros,
Yellow sprout, Japanese lacquer, Enmei-so, Sanshishi, Yangmei, Kakkon,
Psycho, senkyu, thin leaf, succulent, licorice, peony, yokuinin, spicy, half summer, toki, cinnamon, yellow lantern,
Peony skin, gentian, juniper, quintet, senburi, gennoshoko, mahuang, yellow oak, eugon, cypress, turmeric,
Goshuyu, Sage, Oregano, Rosemary, Laurel, Taragon, Onion, Nut Meg, Clove, Wasabi, Savory, Basil, Pepper, Bean Tea, Black Tea, Green Tea, Persimmon Leaf, Coffee, Sugi, Hachiku, Wormwood, Kumasasa, Cuco One or more selected from the plant extract group consisting of various extracts of various plants extracted with water or a lower alcohol or an aqueous solution of a lower alcohol from each plant consisting of, Yabusetetsu, Shiitake Newly found. On the other hand, as compounds, L-cysteine, glutathione, mannitol, gallic acid, potassium sorbate, acetylacetone, triethanolamine, tannic acid, 4-t-butyl-4′-methoxy-
1 selected from the group of compounds consisting of dibenzomethane, 2-hydroxy-4-methylbenzophenone-5-sulfate
It has been newly discovered that one or more than one compound can be used. Therefore, the mucopolysaccharide fragmentation inhibitor according to the present invention is effective for one kind or two or more kinds selected from any one group of the plant various extract groups and the compound group or both groups. It is a mucopolysaccharide fragmentation inhibitor characterized by being contained as a component.

Next, the "active oxygen scavenger" according to the present invention will be described. The "active oxygen scavenger" according to the present invention has industrial utility from the following viewpoints. That is, it is known that active oxygen generated in the living body causes aging and diseases. In vivo, it can be eliminated by enzymes such as superoxide mutase (SOD). However, it is considered that such scavenging ability of active oxygen declines with aging and accelerates aging and diseases.
Further, the superoxide mutase (SOD) is a high molecular weight protein, and its activity is rapidly deactivated, which is not practical. On the other hand, the active oxygen scavenger according to the present invention is present in various extracts of each plant, and has an extremely excellent active oxygen scavenging ability and has stable characteristics. In particular, herbal medicines are complex compounds, and have remarkable properties that they act on various types of active oxygen in a well-balanced manner and that they are excellent in stability against heat and the like. Furthermore, the “active oxygen scavenger” according to the present invention opens up the way of use in the industrial fields of medicine and food.

The amount of each active ingredient (one or more selected from one or both of various plant extracts and various compounds) in the "active oxygen scavenger" according to the present invention (containing The amount) refers to the types of active ingredients and combinations thereof, their intended use, embodiments,
There is no particular limitation because it can be changed according to the usage pattern, the number of times of usage, and the like. In principle, an effective amount will suffice, but generally 0.0001 to 100% by weight, preferably 1 to 10% by weight, based on the composition of active oxygen scavenger can be used. Furthermore, the active ingredient according to the present invention (various plant extracts and various compounds)
Can exhibit the action and effect even with one kind, but by using appropriately combining two or more kinds of active ingredients, an excellent synergistic effect can be achieved. Of course,
The active ingredient according to the present invention (one or more kinds selected from one or both of various plant extracts and various compounds) provides excellent synergism when used in combination with a known active oxygen scavenger. It can also be effective.

The active oxygen scavenging action of various plant extracts and various compounds according to the present invention was measured by a known method. That is, a phosphate buffer containing xanthine and nitroblue tetrazolium is reacted with xanthine oxidase under predetermined conditions, the reaction is stopped with sodium dodecyl sulfate, and then the absorbance at 560 nm is measured to search for the presence or absence of each active ingredient. It was The specific measuring method and the method of calculating the active oxygen inhibition rate will be described in detail in the section of Examples.

Table 4 shows the results of measuring the active enzyme scavenging rate (%) of various extracts and compounds of the plant according to the present invention. The inventors of the present invention have determined that the active oxygen scavenging rate of 20% or more in Table 4 can be used as the “active oxygen scavenger” according to the present invention. That is, as various plant extracts, hibiscus, aloe, rhubarb, yellow sperm, porcupine, life-prolonging grass, sardine,
Yangmei skin, Kakkon, Psycho, senkyu, sojutsu, thin leaf, bukuro, licorice, peony, spicy, half summer, toki, cinnamon, ten medicines, yellow ream, peony skin, gentian, quintet,
Senburi, Gensho, Mao, Huangbai, Apricot kernels, ginger,
Taisou, Squirrel, Chen skin, Turmeric, Shinobi, Kyoutsu, Yellow, Garlic, Sage, Oregano, Rosemary, Laurel, Celery, Thyme, Taragon, Onion,
Nut meg, mace, clove, wasabi, sabori, basil, chili, bean tea, black tea, green tea, persimmon leaf, coffee, passer, bee, wormwood, kumasasa, wolfberry, yabusotetsu,
Selected from various plant extract groups consisting of various extracts of water, lower alcohols or lower alcohol aqueous solutions from plants consisting of shiitake mushrooms, ginkgo, hijiki, squid, konbu, arame, and sea turtles. It was newly discovered that one or more plant extracts are available. On the other hand, as the compound, tannic acid, 2-hydroxy-4-methylbenzophenone-5-
It has been newly discovered that either one or both of the sulfate compounds can be used. Therefore, the "active oxygen scavenger" according to the present invention is one or more selected from one group or both groups of the plant extract group and the compound group that can be used as an active oxygen scavenger. The active oxygen scavenger is characterized by containing as an active ingredient.

Furthermore, the "antioxidant" according to the present invention will be described. "Antioxidant" according to the present invention
Is considered to have industrial utility value from the following viewpoints. That is, sebum is secreted on human skin in order to protect the skin (skin). This sebum is oxidized by ultraviolet rays or the like to become lipid peroxide, and this lipid peroxide is irritating to the skin. In addition, this lipid peroxide attacks the cell membrane to cause damage and various other adverse effects, and these damages are said to be further involved in the aging of human skin. Therefore, it is considered that suppressing the production of the lipid peroxide is effective not only for preventing the skin (skin) from deteriorating but also for preventing aging of the living body. Furthermore, the “antioxidant” according to the present invention opens up the way of application in the industrial fields of medicine and food.

Amount (content) of various active ingredients (various extracts of plants) in the "antioxidant" according to the present invention
Is not particularly limited, because it can be varied depending on the type of active ingredient, the combination thereof, the purpose of use, the embodiment, the form of use, the number of times of use, and the like. In principle, an effective amount will suffice, but generally 0.0001 to 100% by weight, preferably 1 to 10% by weight, based on the composition of the antioxidant can be utilized. Furthermore, the active ingredient according to the present invention (various extracts of plants) can exert the action and effect even with one kind, but it is preferable to use two or more kinds of active ingredients (various extracts of plant) in appropriate combination. As a result, a superior synergistic effect can be achieved. Of course, the active ingredient (various extracts of plants) according to the present invention can also exert an excellent synergistic effect when used in combination with a known “antioxidant”.

The antioxidant activity of the various plant extracts and various test compounds according to the present invention was measured by a known method (Rhodan iron method utilizing autoxidation of linoleic acid). Then, for each extract and each compound of each plant, the presence or absence of each active ingredient was searched. The specific measuring method and the method of calculating the antioxidant activity rate will be described in detail in the section of Examples.

Table 5 shows the results of measuring the antioxidant rate (%) of various extracts and compounds of the plant according to the present invention. The inventors found that the antioxidant rate in Table 5 was 20%.
It was judged that those having a% or more can be used as the "antioxidant" according to the present invention. That is, as various plant extracts, hibiscus, aloe, rhubarb, yellow sperm, arbutus, Enmeisou, Yangmei peel, kakkon, senkyu,
Soujutsu, thin leaf, licorice, peony, Yokuinin,
Chinese cabbage, cinnamon, ten medicines, yellow ream, peony skin, gentian, quintet, senburi, genosho, yellow oak, ginger, sardine, boar, garlic, sage, oregano, rosemary, laurel, celery, thyme, tarragon, nut meg, Mace, cloves, wasabi, sabori, basil, chili, bean tea, black tea, green tea, persimmon leaves, coffee, sugi, bee, wormwood, army chazzle, kumasasa, wolfberry, shiitake mushroom, hijiki, wakame, igusu, konbu It is possible to use, as an active ingredient, one or two or more kinds selected from various plant extract groups extracted with water or lower alcohol or a lower alcohol aqueous solution from each plant consisting of, Japanese porridge, sea turtle, blue seaweed, and Nori seaweed. Newly found. Therefore, the antioxidant according to the present invention is characterized by containing, as an active ingredient, one or more selected from the above-mentioned various plant extract groups.

The "antioxidant" according to the present invention can be applied not only to cosmetics but also to various related technical fields such as various medicines, various medicines, chemicals and fertilizers. In addition, all of the active ingredients according to the present invention have low toxicity and irritation to the skin, high stability to heat and light, and high stability to various cosmetic bases and cosmetic additives. It has characteristics.

Furthermore, "cosmetics" according to the present invention
Will be described. The "cosmetic" according to the present invention is considered to have industrial utility value from the following viewpoints.
In other words, as mentioned above, "mucopolysaccharide fragmentation inhibitor",
Since it is composed of a cosmetic containing one or more of "active oxygen scavenger" and "antioxidant" as an active ingredient,
Actions and effects that can be exhibited based on the types of various plant extracts and the types of compounds that are incorporated into cosmetics can be directly used as the actions and effects of cosmetics. Therefore, the cosmetic according to the present invention can provide a skin (skin) anti-aging cosmetic as a basic action / effect.

The blending amount (content) of various active ingredients (various extracts of plants and groups of compounds) according to the present invention with respect to various cosmetics is the kind of the above-mentioned active ingredients and the combination thereof, and the kind and the cosmetics thereof. Purpose, embodiment,
There is no particular limitation as it can be varied depending on the usage form, the number of uses, etc. of the cosmetic. In principle, an effective amount should be present, but generally 0.0001 to 100% by weight can be used in the cosmetic composition, preferably 0.01 to 10% by weight, and especially 0. The optimum amount is 1 to 5.0% by weight. In particular, when applied to cosmetics as a cosmetic composition prepared at the time of use, any one selected from the mucopolysaccharide fragmentation inhibitor, the active oxygen scavenger and the antioxidant according to the present invention or 2 or more is 10
It will be used at a high compounding rate including 0% by weight.

Furthermore, the active ingredient (various plant extracts and compounds) according to the present invention can exert its action and effect even if it is selected from the group consisting of various plant extracts and compounds. An excellent synergistic effect can be achieved by appropriately combining two or more kinds of active ingredients. Naturally, the active ingredients (various extracts of plants and various compounds) according to the present invention are known "mucopolysaccharide fragmentation inhibitor" and "active oxygen scavenger".
It goes without saying that an excellent synergistic effect can be obtained by using in combination with one or two or more selected from "and antioxidant".

The application range of the cosmetics according to the present invention is not particularly limited. That is, the present invention can be applied to all cosmetics that can utilize each action and effect according to the action and effect of the active ingredient of the present invention. For example, one kind or two or more kinds of the active ingredients according to the present invention are blended with various cosmetic bases, and various basic cosmetics such as creams, emulsions, lotions, packs, face washes, foundations, and blushers. , Various make-up agents such as lipstick and white powder, hair styling agents, hair nourishing agents, shampoos, conditioners, hair cosmetics, soaps, nail polish,
Widely applicable to other cosmetics such as perfumes, colognes, etc. In addition, the embodiment of the various cosmetics,
Solution, emulsion, ointment, oil, wax, sol,
It can be applied in various forms such as gel, powder, and spray (aerosol).

Various extracts of each plant according to the present invention are
Since they are all composites, they act on a wide variety of active oxygen in a well-balanced manner, have good thermal stability, and have the outstanding property that a highly safe cosmetic composition can be provided.

[0032]

The various plant extracts and compounds according to the present invention are highly safe and stable due to their excellent inhibitory action on mucopolysaccharide fragmentation by active oxygen and ultraviolet light on the inhibition of mucopolysaccharide fragmentation. It is possible to provide a "mucopolysaccharide fragmentation inhibitor" with certainty, and thus skin (skin)
Aging prevention can be achieved.

The various plant extracts and compounds according to the present invention are highly safe and stable "active oxygen scavengers" due to their excellent active oxygen scavenging action.
It is possible to provide aging prevention and disease prevention. In particular, it can exert a remarkable action effect in preventing aging of the skin (skin).

Further, the various plant extracts and compounds according to the present invention suppress the production of substances (for example, lipid peroxide) which have a bad effect due to active oxygen due to their excellent antioxidative action, and thus are safe. It is possible to provide a highly stable "antioxidant", and thus the skin (skin)
It is possible to prevent the deterioration of the condition and prevent the aging of the living body.

[0035]

EXAMPLES Next, the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples. In addition, the part in an Example shows a weight part unless there is particular notice.

(1) Preparation of plant extract. As a raw material for the plant extract, 10 g of dried and powdered products of each plant were used. First, for the preparation of "water extract of plant", 10 g of the above raw material was put into a cylindrical filter paper,
The filtrate was obtained by immersing in 100 ml of ion-exchanged water and heating and extracting at 60 ° C. for 8 hours. This operation was repeated 4 times, and all the filtrates were combined and freeze-dried to obtain a water extract (dry powder) of the plant. Next, ethanol was used as the lower alcohol for the preparation of the “plant lower alcohol extract” and the “plant lower alcohol aqueous solution extract”. Second
For the preparation of "50% ethanol aqueous solution extract of plant", 50% ethanol aqueous solution was used in place of water in the extraction operation in the water extract preparation.
Then, during the extraction operation, extraction was performed under reflux conditions. All extracts were combined, ethanol was distilled off as much as possible, and the concentrated extract was freeze-dried.
% Aqueous ethanol solution extract "was obtained. Thirdly, for the preparation of "plant ethanol extract", after extracting with a Soxhlet extractor for 8 hours, the solvent is distilled off and the extract is powdered to obtain "plant ethanol extract". It was

(2) Reagents. As a sample of mucopolysaccharide, sodium hyaluronate (of microbial origin) (manufactured by Meiji Seika Co., Ltd.) was used. On the other hand, as a positive target of a hyaluronic acid fragmentation-inhibiting substance by ultraviolet rays, 4-t-butyl-4′-methoxy-dibenzomethane (hereinafter referred to as “parsol 1789”) (GI
VADAN), 2-hydroxy-4-methylbenzophenone-5-sulfate (hereinafter referred to as "ASL-24S") (manufactured by Shonan Chemical Co., Ltd.) were used.

(3) Retrieval test for inhibiting mucopolysaccharide fragmentation. Sodium hyaluronate (commercially available) was used as a sample of mucopolysaccharide. As plant extracts, we searched extracts from 77 types of plants such as crude drugs, spices and tea. In addition, with respect to 11 kinds of compounds, a search test for a mucopolysaccharide fragmentation-inhibiting action was similarly conducted.

(A) Active oxygen (ascorbic acid-iron system)
A method for measuring the inhibitory effect on hyaluronic acid fragmentation by. To 0.45 ml of 0.3 M phosphate buffer (pH 5.3) containing 0.04% sodium hyaluronate, 0.05 ml of 1% test sample aqueous solution (aqueous solution of various plant extracts or compound) and ascorbine After adding 0.025 ml of acid and 0.025 ml of 1 mM ferric chloride aqueous solution and incubating at 37 ° C. for 24 hours, 0.2 ml of the reaction solution was taken out, and 0.04 M sodium acetate containing 0.1% albumin was added thereto. Add 2.0 ml of 0.08 M acetate buffer (pH 3.75) and stir well. 5
After standing for minutes, the turbidity of the produced complex of hyaluronic acid and albumin is measured as the absorbance (Esr) at 600 nm. (Amount of residual hyaluronic acid)

In the measurement of the amount of hyaluronic acid used in this method,
Since a complex may be formed between the plant extract and albumin and albumin, the turbidity when only sodium hyaluronate was removed in the above measurement operation as a blank, that is, the absorbance (Eb) was measured and corrected.

The original amount of hyaluronic acid used in the test sample was the turbidity of the complex with albumin at 600 nm when the fragmentation operation of hyaluronic acid in the ascorbic acid-iron system was omitted from the above measurement operations. Absorbance (Eso)
It was measured by.

The fragmentation inhibitory rate (%) of hyaluronic acid is calculated by measuring the fragmentation of hyaluronic acid by the ascorbic acid-iron system by the above-mentioned method [“the amount of residual hyaluronic acid (Esr)”]. The ratio (%) to the original amount of hyaluronic acid [“Original hyaluronic acid amount (Eso)”] used in the sample was calculated by [Equation 1].

[0043]

[Equation 1]

(B) A method for measuring the inhibitory effect on the fragmentation of hyaluronic acid by ultraviolet rays (UV-A). To each well of a 12-well multi-dish (22 mmφ), 1 ml of 0.3 M phosphate buffer (pH 5.3) containing 0.04% sodium hyaluronate was dispensed, and a 1% test sample aqueous solution (various extractions of plants) 0.1 ml of an aqueous solution of water and a water-soluble compound) to form a hyaluronic acid layer. Meanwhile, 0.5 m of 2-ethylhexanoic acid cetostearyl alcohol ester was formed on the hyaluronic acid layer.
1 Overlay and dissolve the oil-soluble material. Then, ultraviolet rays were irradiated at 37 ° C. for 7 days with an ultraviolet lamp (Dermaray FL20SBLB / Toshiba Medical Supplies Co., Ltd.). The wavelength of the ultraviolet rays required for this irradiation and the total energy amount for irradiation for 7 days are 365 nm (12.0 KJ / cm ² ) and 306 nm.
nm (67.0 J / cm ² ).

0.2 ml of the liquid of the aqueous layer was taken out from each well, and 2.0 ml of 0.04 M sodium phosphate / 0.08 M acetate buffer (pH 3.75) containing 0.1% albumin was added to the well. Stir well. After standing for 5 minutes, the turbidity of the produced complex of hyaluronic acid and albumin was adjusted to 60
It was measured as the absorbance (Esr) at 0 nm. (Amount of residual hyaluronic acid) In addition, all tests are performed aseptically,
The hyaluronic acid solution was sterilized by an autoclave. 12
The lid of the hole multi-dish was removed, covered with a plastic bag, and irradiated with ultraviolet rays.

In the measurement of the amount of hyaluronic acid used in this method,
Since a complex may be formed between the plant extract and albumin and albumin, the turbidity when only sodium hyaluronate was removed in the above method as a blank, that is, the turbidity (Eb) was measured and corrected.

The original amount of hyaluronic acid used in the test sample was determined by measuring the turbidity of the complex with albumin at 600 nm when the fragmentation operation of hyaluronic acid in the UV-A system was omitted from the measurement operations. It was measured as absorbance (Eso). (Original amount of hyaluronic acid)

The calculation of the fragmentation inhibition rate (%) of hyaluronic acid by the ultraviolet ray system is carried out in the same manner as the calculation of the fragmentation inhibition rate of hyaluronic acid by the ascorbic acid-iron system (A). The original test amount of hyaluronic acid used for the test sample of the amount of hyaluronic acid (“residual amount of hyaluronic acid (Esr)”) measured by UV (UV-A system) fragmentation of hyaluronic acid (“the original sample Hyaluronic acid amount (Es
o) ”) was calculated and calculated by [Equation 2].

[0049]

[Equation 2]

[Table 1] shows the results of the hyaluronic acid fragmentation-inhibiting action of each plant water extract and each compound. [Table 2] shows the results of the hyaluronic acid fragmentation-inhibiting action of the 50% ethanol aqueous solution extract of each plant and each compound.
[Table 3] shows the results of the hyaluronic acid fragmentation-inhibiting action of each plant ethanol extract and each compound.

In Tables 1 to 3, "ASA-Fe system" in the column of fragmentation method means the inhibitory effect on the fragmentation of hyaluronic acid by active oxygen (ascorbic acid-iron system). The result (hyaluronic acid fragmentation inhibition rate) measured by the method is shown, and "UV-A system" means ultraviolet rays (U
The results (hyaluronic acid fragmentation inhibitory rate) measured by the method for measuring the inhibitory effect on the fragmentation of hyaluronic acid by VA) are shown respectively. Moreover, all the numbers in the table indicate the inhibition rate (%).

[0052]

[Table 1]

[0053]

[Table 2]

[0054]

[Table 3]

From these results, water extracts of various plants,
A mucopolysaccharide fragmentation-inhibiting action is observed in various extracts of ethanol aqueous solution and ethanol extract and compounds.

Retrieval test of active oxygen scavenging action. (4) A method for measuring the action of eliminating active oxygen. First, the following coloring reagent solution, enzyme solution, blank solution and reaction stop solution are prepared. Color developing solution: Dissolve xanthine in 0.04 mmol / l and nitroblue tetralysodium (NO ₂ -TB) in 0.1 M phosphate buffer (pH 8.0) at 0.24 mmol / l. Enzyme solution: 0.1 M phosphate buffer (pH 8.0) in xanthine oxidase (butter milk / Butter m
(from ilk) is dissolved at a concentration of 0.049 units / ml. Blank solution: 0.1 M phosphate buffer (pH 8.0) Reaction stop solution: 69 mM sodium dodecyl sulfate

The "enzyme / sample sample (S)" was prepared by adding 0.1 ml of a 1% aqueous sample solution (aqueous solution of various plant extracts or compound) to 1.0 ml of the coloring reagent solution and 1.0 m of the enzyme solution.
1 and the mixture is heated at 37 ° C. for exactly 20 minutes, and then 2.0 ml of a reaction stop solution is added to stop the reaction. After stopping the reaction,
The absorbance (Es) at 560 nm is measured. Also,
The “enzyme sample (B)” is 0.1 m of distilled water instead of the 1% test sample (aqueous solution of various extracts or aqueous solution of compounds).
l was added and the same operation as in the case of the measurement of the above-mentioned “enzyme / sample specimen” was carried out, and the absorbance at 560 nm (E
b) is measured.

On the other hand, the "enzyme-free sample sample (SB)" was prepared by adding 1.0 ml of a 1% sample aqueous solution (aqueous solution of various extracts of plant or aqueous solution of compound) to 1.0 ml of color developing solution and 1.0 ml of blank solution. In addition, after heating at 37 ° C for exactly 20 minutes, 2.0 ml of a reaction stop solution was added to stop the reaction, and then 5
The absorbance (Esb) at 60 nm is measured. Also,
The “enzyme-free sample (BB)” was prepared by adding 0.% distilled water instead of a 1% sample (an aqueous solution of various plant extracts or an aqueous solution of a compound).
1 ml is added and the same operation as the above-mentioned "non-enzyme / sample sample" is performed to measure the absorbance (Ebb) at 560 nm. Then, the active oxygen scavenging rate (%) was calculated and obtained by the following [Equation 3].

[0059]

[Equation 3]

[Table 4] shows the active oxygen scavenging rate (%) of each sample thus measured.

[0061]

[Table 4]

From Table 4, various extracts and compounds of the plant according to the present invention are recognized to have an excellent action of eliminating active oxygen.

Antioxidant retrieving test. (5) Antioxidant measuring method (by the Rodan iron method). 0.2 ml of 0.01% test sample solution (plant extract solution or compound solution) was added to 1 ml of 2 × 10 ⁻² M linoleic acid stock solution.
0.8 ml of 1M phosphate buffer (pH 7.0) was added,
The mixture was sealed and reacted at 37 ° C. for 48 hours,
The amount of peroxide was measured by the Rhodan iron method. 0.1 ml of the mixed solution after the reaction was taken out, and 4.7 ml of 75% ethanol and 0.3% of 30% ammonium thiocyanate solution were added to this.
1 ml was added and stirred, and then 0.1 ml of a 3.5% hydrochloric acid solution containing 2 × 10 ⁻² M ferrous chloride was added and the mixture was stirred 5
The absorbance (As) at 00 nm was measured. As a negative control, 500 n when distilled water was used instead of the 0.01% test sample solution (plant extract solution or compound solution)
The absorbance (Ac) at m was measured. Also, as a positive control, 500 nm when 0.01% Emix solution (Tween 20 added and dissolved in water) was added
Absorbance (Ab) is measured. Then, the antioxidant activity (%) is calculated by [Equation 4].

[0064]

[Equation 4]

[Table 5] shows the respective antioxidant activity rates (%) of various extracts and compounds of the plant according to the present invention.

[0066]

[Table 5]

From the results shown in [Table 5], it is recognized that various plant extracts and compounds have antioxidant activity.

Next, examples (prescription examples) of cosmetics using various plant extracts according to the present invention will be shown. The cosmetic material according to the present invention is not limited by this embodiment.

(6-1) Cream (W / O Type) Oily Component: (wt%) Plant Extract 0.1 Cetanol 8.0 Jojoba Oil 5.0 Squalane 37.5 Beeswax 6.0 Emulsifier: Lipophilic Mono Glycerin stearate 2.0 Polyoxyethylene sorbitan monolaurate (20.EO) 2.0 Perfume proper amount preservative proper amount aqueous phase: dipropylene glycol 2.0 glycerin 3.0 purified water 30.0 [production method] The components of the aqueous phase are mixed, heated and kept at 70 ° C. to form the aqueous phase part. On the other hand, other ingredients are mixed, heated and melted at 70 ° C.
As the oil phase part. This oil phase part was added to the above-mentioned water phase part to carry out preliminary emulsification, and homogenized with a homomixer.
Cool to ℃ to obtain the product cream.

(6-2) Emulsion Oil Phase (wt%) Plant Extract 0.1 Cetanol 5.0 Squalane 10.0 Cetyl Octanoate 10.0 Emulsifier: Sorbitan Sesquioleate 3.0 Polyoxyethylene hydrogenated castor oil (50 EO) 3.0 Fragrance Amount Preservative Amount Aqueous phase: Glycerin 3.0 Purified water 65.0 [Production Method] Mix components of aqueous phase and heat to 70 ° C to form aqueous phase part . On the other hand, other components are mixed, heated and dissolved to 70 ° C. to obtain an oil phase part. This oil phase part is added to the above-mentioned water phase part and emulsified, and cooled to 30 ° C. to obtain a product emulsion.

(6-3) Creamy foundation Oil phase: (wt%) Plant extract 0.2 Stearic acid 5.0 Glycerin monostearate 2.5 Cetanol 1.0 Propylene glycol monolaurate 3.0 Squalane 7. 0 Cetyl octoate 8.0 Aqueous phase: Purified water 53.3 Triethanolamine 1.2 Solbit 3.0 Preservative Suitable amount Pigment: Titanium oxide 8.0 Kaolin 5.0 Talc 2.0 Bentonite 1.0 Coloring pigment Suitable amount Fragrance Suitable amount [Production method] Mix pigments and grind. An aqueous phase is prepared, the mixed pigment is added thereto to disperse, and then the mixture is heated to 75 ° C. The oil phase is prepared and heated to 80 ° C. The oil phase was added to the water phase with stirring, emulsified and then cooled, and the fragrance was added at 50 ° C, and further 3
Cool to 0 ° C. to obtain the product.

(6-4) Lipstick (wt%) Base: Plant extract 0.2 Castor oil 45.0 Hexadecyl alcohol 25.0 Beeswax 5.0 Candelilla wax 7.0 Carnauba wax 6.0 Lanolin 4. 0 Colorant: Titanium oxide 2.0 Colorant Appropriate amount Perfume Appropriate amount [Production Method] The base material is melted by heating and mixed uniformly. The coloring material is added to this, and the mixture is kneaded by a roll mill to uniformly disperse the coloring material, and then remelted to add a perfume, defoamed, poured into a mold, and rapidly cooled to harden.

(6-5) Solid Foundation Pigment: (wt%) Titanium oxide 13.0 Kaolin 25.0 Talc 45.0 Red iron oxide 0.8 Yellow iron oxide 2.5 Black iron oxide 0.1 Binder: Plant Extract 0.1 Squalane 10.0 Sorbitan sesquioleate 3.5 Preservative Appropriate amount Perfume Appropriate amount [Production Method] Mix pigments and grind with a grinder. This is transferred to a high speed blender, the binder and preservative are mixed, and a pigment is added to the homogenized mixture to further homogenize it. This is processed with a crusher and compression molded.

[0074] (6-6) Powder-like pack     Powder: (wt%)       Kaolin Make the whole 100.       Talc 20.0       Oxidized Aene 19.0     Oil content:       Olive oil 2.0     Dispersant:       Polyoxyethylene sorbitan monolaurate           (40 EO) 1.0     Moisturizer:       Glycerin 8.0     Preservative:       Ethylparaben Suitable amount     Plant extract 10.0

[0075]

EFFECTS OF THE INVENTION Based on the characteristics of mucopolysaccharide fragmentation-inhibiting action, active oxygen scavenging action, and antioxidant action of various plant extracts and various compounds, various aging and diseases caused by active oxygen are effective. It is possible to provide a mucopolysaccharide fragmentation inhibitor, an active oxygen scavenger, and an antioxidant which can be prevented and are excellent based on these properties. Moreover, when the mucopolysaccharide fragmentation inhibitor, the active oxygen scavenger and the antioxidant are derived from various plant extracts, the plants contain many crude drugs, etc. Since it is a natural product and is a compound, it is safe and stable to heat, has few side effects, and has broad applications not only in cosmetics but also in the technical fields of medicine and food. Has a remarkable effect.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI A61P 17/16 A61P 17/16 (72) Inventor Hiroshi Tanaka 1-1-11 Ebie, Fukushima-ku, Osaka-shi, Osaka Naris Cosmetics Co., Ltd. (72) Inventor Atsushi Shiba 1-11-17 Ebie, Fukushima-ku, Osaka-shi, Osaka Naris Cosmetics Co., Ltd. (56) Reference JP-A-3-209330 (JP, A) JP-A-1-128933 (JP , A) JP 2-11520 (JP, A) JP 3-190809 (JP, A) JP 2-117686 (JP, A) JP 3-93710 (JP, A) JP 60-87771 (JP, A) JP-A-2-221229 (JP, A) JP-A-3-151315 (JP, A) JP-A-4-169535 (JP, A) JP-A-62-255415 (JP, A) JP 61-28078 (JP, A) JP 64-83022 ( P, A) JP 63-162611 (JP, A) JP 64-16709 (JP, A) JP 62-207289 (JP, A) JP 60-78910 (JP, A) JP JP-A-54-138130 (JP, A) JP-A-64-47709 (JP, A) JP-A-3-236305 (JP, A) JP-A-3-178916 (JP, A) JP-A-2-111710 (JP , A) JP 5-238925 (JP, A) JP 5-320037 (JP, A) JP 55-153711 (JP, A) JP 3-101610 (JP, A) JP 1-83008 (JP, A) JP 3-176407 (JP, A) JP 3-93782 (JP, A) JP 2-264727 (JP, A) JP 2-243632 (JP, A) Japanese Patent Publication No. 54-976 (JP, B2) Japanese Patent Publication No. 4-14642 (JP, B2) International Publication 92/07544 (WO, A1) (58) Fields investigated (Int.Cl. ⁷ , DB name) A61K 7/00 A61K 35/78 A61K 31/00

Claims (2)

(57) [Claims] 1. A variety of plants extracted from water, a lower alcohol, or a lower alcohol aqueous solution from each plant consisting of Ryutan, Hibiscus, Yellow spice, Chinese pepper, Gentian, Goshuyu, Oregano, Laurel, Taragon, Onion, Wasabi, Sabori and Sugi. A mucopolysaccharide fragmentation inhibitor comprising one or two or more selected from plant extracts and gallic acid as an active ingredient. 2. A cosmetic for inhibiting mucopolysaccharide fragmentation, which comprises the mucopolysaccharide fragmentation inhibitor according to claim 1 as an active ingredient.