JP2003113068A - Skin cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a moisturizing agent, an antioxidant, an antiaging agent, an antiinflammatory agent and a skin cosmetic by finding the substances having moisture-retaining property, active oxygen scavenging action, antiaging action or antiinflammatory action from natural substances having high safety. SOLUTION: A moisturizing agent, an antioxidant agent, an antiaging agent, an anti-inflammatory agent and skin cosmetics are combined with an extract from a maple plant, particularly Acer palmatum, having the moisturizing action, the active oxygen-scavenging action, the radical-scavenging action, the collagen- production-promoting action, the collagenase-inhibitory production-promoting action, the collagenase-inhibitory action, the fibroblast cell proliferation action, the elastase-inhibitory action the estrogen-like action, the hyaluronidase inhibitory action, the cyclic AMP phosphodiesterase inhibitory action or the histamine release inhibitory action.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a moisturizing agent, an antioxidant, an anti-aging agent and an anti-inflammatory agent containing a plant extract as an active ingredient,
It also relates to skin cosmetics containing a plant extract.

[0002]

2. Description of the Related Art There are various types of onset mechanisms for rough skin and skin aging, and the main onset mechanisms and their countermeasures are as follows.

(1) Decrease in moisturizing function The moisturizing function of the skin deteriorates due to aging and various environmental factors. Skin with reduced moisturizing function becomes dry and lumpy and loses elasticity. Such dry skin may cause various skin diseases such as atopic dermatitis, and further may cause aging of the skin such as spots and wrinkles. In order to prevent skin dryness, glycerin, propylene glycol, 1,3-butylene glycol and other polyhydric alcohols, oils and fats components, amino acids, proteins, polysaccharides, etc. have been conventionally used. Is problematic in terms of usability, sustainability of moisturizing effect, and safety.

(2) Effect of Active Oxygen / Radical Radicals In recent years, active oxygen has been attracting attention as a factor for oxidizing biological components, and its adverse effect on living organisms has become a problem. Active oxygen is generated in the process of energy metabolism in living cells, and is a superoxide (that is, a superoxide anion (・ O
_2- ), hydrogen peroxide (H ₂ O ₂ ), singlet oxygen ( ¹ O ₂ ), hydroxy radical (.OH), etc. These active oxygens are essential for the bactericidal mechanism of phagocytes and play an important role in the removal of viruses and cancer cells, but excessive production of active oxygen attacks the biomolecules that make up the membranes and tissues in the body. , Induce various diseases. For example, active oxygen is believed to decompose, denature or cross-link biological tissues such as collagen, or to oxidize oils and fats to produce lipid peroxides that damage cells, and these are caused by active oxygen. Is considered to be a cause of skin aging such as wrinkle formation and reduced elasticity.

[0005] In the living body, the radical initially generated based on oxygen is superoxide, and other radicals such as hydroxy radical are generated through superoxide. Intracellular superoxide is converted to hydrogen peroxide by intracellularly produced superoxide dismutase (SOD), but the amount of SOD decreases with age, and due to the decrease in SOD, intracellular superoxide is reduced. As the concentration increases, superoxide becomes harmful to the living body. Therefore, in order to compensate for the decrease in the amount of SOD, SOD itself, tocopherols, augon extract, etc. are used as SOD-like agents, but these SOD-like agents have problems in safety and the like. .

(3) Decrease of extracellular matrix constituents / dermis of degenerated skin The dermis and epidermis are composed of epidermal cells, fibroblasts, and elastin, collagen, hyaluronic acid, etc. which are outside these cells and support the skin structure. It is composed of an extracellular matrix, and in young skin, the interaction of these skin tissues maintains homeostasis to ensure water retention, flexibility, elasticity, etc. It is kept fresh. However, due to the influence of certain external factors such as irradiation with ultraviolet rays, remarkable dryness of air, excessive skin washing, and the progress of aging, elastin and collagen, which are the main components of the extracellular matrix, It reduces the production of hyaluronic acid and causes denaturation and decomposition. As a result, the keratin begins to peel abnormally, the skin loses its tension and luster, and the skin becomes rough and wrinkles and other aging symptoms. As described above, changes associated with skin aging, that is, wrinkles, dullness, loss of texture, decrease in elasticity, etc., are associated with a decrease in dermal extracellular matrix components such as elastin, collagen, hyaluronic acid, and degeneration. There is.

In recent years, it has been pointed out that the matrix protease is particularly involved as a factor inducing this change. Among matrix-type proteases, collagenase, that is, MMP-1 (matrix metalloprotease-1) is known as an enzyme that decomposes type I and III collagens, which are the main constituents of the dermal extracellular matrix of skin, and its expression Is greatly increased by the irradiation of ultraviolet rays, which is one of the causes of the decrease / denaturation of collagen by ultraviolet rays, and is considered to be a major factor such as the formation of wrinkles on the skin. Therefore, inhibition of collagenase activity is important for preventing and improving skin aging.

On the other hand, there are various causes and mechanisms of inflammatory diseases such as contact dermatitis (rash), psoriasis, pemphigus vulgaris, and other various skin diseases accompanied by rough skin.
It is known that the cause thereof is platelet aggregation and histamine release by hyaluronidase and cyclic AMP phosphodiesterase.

[0009] Platelet aggregation causes activation of phospholipase A2 in the arachidonic acid cascade, and leukotriene B4, prostaglandin E2, and the like released thereby induce an inflammatory reaction. Therefore, substances that inhibit or suppress platelet aggregation prevent or prevent allergic or inflammatory diseases.
Attempts have been made to treat it, and aspirin, ticlopidine, sulfipyrazone, etc. have been used as such platelet aggregation inhibitors. However, all of these substances have side effects, which has been a problem in terms of safety.

Further, the aggregation of platelets is related to the concentration of cyclic AMP in platelets, and when cyclic AMP is decomposed by cyclic AMP phosphodiesterase and the concentration of cyclic AMP decreases, platelets are likely to aggregate. Therefore, it is considered that platelet aggregation can be prevented by suppressing the action of cyclic AMP phosphodiesterase to prevent the decrease of the cyclic AMP concentration.

[0011]

Accordingly, the present invention is, firstly, to find a substance having a moisturizing action from among highly safe natural products, and to provide a moisturizing agent containing the substance as an active ingredient. To aim.

Secondly, the present invention finds a substance capable of scavenging active oxygen and in-vivo radicals from a highly safe natural product through an active oxygen scavenging action or a radical scavenging action, and it is effective. It is intended to provide an antioxidant as a component. Moreover, it aims at providing the active oxygen scavenger and radical scavenger which used the said substance as an active ingredient.

[0013] Thirdly, the present invention is, thirdly, from among highly safe natural products, aging of the skin through collagen production promoting action, collagenase inhibiting action, fibroblast proliferation action, elastase inhibiting action or estrogen-like action. It is an object of the present invention to find a substance capable of preventing and improving aging and to provide an anti-aging agent containing it as an active ingredient. In addition, it is also an object of the present invention to provide a collagen production promoter, a collagenase inhibitor, a fibroblast proliferation agent, an elastase inhibitor and an estrogen-like agent which contain the substance as an active ingredient.

Further, the present invention is, fourthly, a substance which can prevent or ameliorate an inflammatory disease from among highly safe natural products through a hyaluronidase inhibitory action, a cyclic AMP phosphodiesterase inhibitory action or a histamine release inhibitory action. And an object thereof is to provide an anti-inflammatory agent containing the same. Moreover, it aims at providing the hyaluronidase inhibitor, cyclic AMP phosphodiesterase inhibitor, and histamine release inhibitor which used the said substance as an active ingredient.

Fifthly, the present invention finds a substance having a moisturizing action, an antioxidant action, an anti-aging action or an anti-inflammatory action from among highly safe natural products, and it is found that the skin is rough, the skin is aging, and Prevention of various skin diseases including inflammatory diseases
It is intended to provide a skin cosmetic useful for improvement.

[0016]

In order to achieve the above object, the moisturizing agent, antioxidant, anti-aging agent and anti-inflammatory agent of the present invention contain an extract from a plant belonging to the genus Acer, as an active ingredient. It is characterized by doing. In the moisturizing agent, antioxidant, anti-aging agent and anti-inflammatory agent of the present invention, it is preferable that the plant belonging to the genus Acer is Acer palmatum. Further, in the antioxidant of the present invention, it is preferable that the extract has an active oxygen scavenging action and / or a radical scavenging action. Further, in the anti-aging agent of the present invention, the extract is one or more selected from the group consisting of a collagen production promoting action, a collagenase inhibiting action, a fibroblast proliferation action, an elastase inhibiting action and an estrogen-like action. It is preferable to have an action. Further, in the anti-inflammatory agent of the present invention, it is preferable that the extract has one or more actions selected from the group consisting of a hyaluronidase inhibitory action, a cyclic AMP phosphodiesterase inhibitory action and a histamine release inhibitory action. Further, in order to achieve the above-mentioned object, the skin cosmetic of the present invention is characterized by containing an extract from a plant belonging to the genus Acer. In the skin cosmetic of the present invention,
The plant belonging to the genus Acer palma is Acer palma.
tum) is preferred. In addition, the skin cosmetic of the present invention may contain a whitening agent in addition to the above extract,
Suitable whitening agents include one or more whitening agents selected from the group consisting of ascorbic acid or its derivatives, placenta extract, kojic acid, rucinol, ellagic acid and chamomile extract.

[0017]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below. In the present invention, the "extract from a plant belonging to the genus Acer genus" means an extract obtained by using a plant belonging to the genus Acer as an extraction raw material, a diluted solution or a concentrate of the extract, and an extract obtained by drying. The dried product, or the crude product or the purified product thereof is included.

The plant used as an extraction raw material is maple (Ac
er) is not particularly limited as long as it belongs to the genus Aer, and examples of plants belonging to the genus Acer include Acer aid.
zuense, Acer amoenum, Acer argutum, Acer buerger
ianum (maple tree), Acer campestre (maple tree), Acer carpinifolium (cypress tree), Acer crata
egifolium, Acer diabolicum, Acer distylum, Acer ginnala
(Acer japonicum), Acer japonicum (Acer maple), Acer mono (Acer maple), Acer negu
ndo (Acer nigrum), Acer nigrum (black
Maple), Acer nikoense, Acer ni
pponicum, Acer palmatum, Acer platanoides, Acer p
seudoplatanus, Acer pycnanth
um, Acer rufinerve,
Acer saccharum, Acer sieboldianum
(Acer palmatum), Acer tschonoskii (Mine maple), Acer ukurunduense (Agarabana), etc., among which Acer palmatum (Acer palmatum)
Is preferably used as the extraction raw material. The Japanese maple is a deciduous subtree belonging to the maple family, and is also called a Japanese maple, a Japanese maple, a Japanese maple, or the like. Japanese maple is distributed in Shikoku, Kyushu, southern Korea, eastern China, etc., and is easily available from these areas.

The constituent parts of the plant used as the extraction raw material are not particularly limited. For example, constituent parts such as leaves, branches, roots, bark and seeds can be used as the extraction raw material. In particular, it is preferable to use the leaf portion as an extraction raw material.

Moisturizing action, active oxygen scavenging action, radical scavenging action, collagen production promoting action, collagenase inhibitory action, contained in an extract from a plant belonging to the genus Acer,
Fibroblast proliferation, elastase inhibition, estrogen-like action, hyaluronidase inhibition, cyclic
The details of the substance having an AMP phosphodiesterase inhibitory action or a histamine release inhibitory action are not known, but it can be obtained from plants belonging to the genus Acer genus by an extraction method generally used for extracting plants. For example, it can be obtained by drying the extraction raw material and then pulverizing it as it is or by using a coarse crusher and subjecting it to extraction with an extraction solvent. At this time, the extraction raw material may be dried in the sun,
You may perform using the dryer normally used. In addition, plants belonging to the genus Acer may be subjected to pretreatment such as defatting with a non-polar solvent such as hexane and benzene and then used as an extraction raw material. By performing pretreatment such as degreasing,
Extraction treatment with a polar solvent from a plant belonging to the genus Acer can be efficiently performed.

As the extraction solvent, it is preferable to use water, a hydrophilic organic solvent or a mixture thereof at room temperature or at a temperature not higher than the boiling point of the solvent.

The water that can be used as the extraction solvent includes pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water and the like, as well as those that have been subjected to various treatments.
Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering and the like.
Therefore, the water that can be used as the extraction solvent in the present invention also includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline and the like.

Examples of the hydrophilic organic solvent which can be used as the extraction solvent include, for example, methanol, ethanol, propyl alcohol, isopropyl alcohol and the like having 1 to 5 carbon atoms.
And lower aliphatic ketones such as acetone and methyl ethyl ketone; and polyhydric alcohols having 2 to 5 carbon atoms such as 1,3-butylene glycol, propylene glycol and glycerin.

When a mixed liquid of water and a hydrophilic organic solvent is used as an extraction solvent, water is used in the case of a lower alcohol.
1 to 90 parts by weight with respect to 0 parts by weight, 1 to 40 parts by weight with respect to 10 parts by weight of water in the case of a lower aliphatic ketone, and 10 to 90 parts by weight with respect to 10 parts by weight of water with a polyhydric alcohol. It is preferable to add.

In the present invention, moisturizing action, active oxygen scavenging action, radical scavenging action, collagen production promoting action, collagenase inhibiting action, fibroblast proliferation action, elastase inhibiting action, estrogen-like action, hyaluronidase inhibiting action, cyclic AMP phosphodiesterase inhibiting action It is not necessary to employ a special extraction method for obtaining an extract having an action or a histamine release inhibitory action, and it is possible to perform extraction using any device at room temperature or under reflux heating.

Specifically, the extraction raw material is put into a treatment tank filled with an extraction solvent, and if necessary, the mixture may be agitated at intervals of 3 times.
After the soluble component is eluted by standing for 0 to 2 hours, the solid matter is removed by filtration, the extraction solvent is distilled off from the obtained extract, and the extract is obtained by drying. The amount of the extraction solvent is usually 5 to 15 times the amount of the extraction raw material (weight ratio),
When water is used as an extraction solvent, the extraction conditions are usually 50 to 95 ° C. and about 1 to 4 hours. When a mixed solvent of water and ethanol is used as the extraction solvent,
It is usually at 40 to 95 ° C. for about 30 minutes to 4 hours.

The obtained extract is diluted, concentrated, dried according to a conventional method to obtain a diluted solution or concentrated solution of the extracted solution, a dried product of the extracted solution, or a crudely purified product or a purified product thereof. You may perform processing, such as refinement | purification.

The obtained extract may be used as it is as a moisturizer, antioxidant, anti-aging agent, anti-inflammatory agent, active oxygen scavenger, radical scavenger, collagen production promoter, collagenase inhibitor, fibroblast proliferation agent. , An elastase inhibitor, an estrogen-like agent, a hyaluronidase inhibitor, a cyclic AMP phosphodiesterase inhibitor or a histamine release inhibitor, but a concentrate or a dried product thereof is easier to use. Formulation of an extract from a plant belonging to the genus Acer can be performed according to a conventional method. In the case of formulation, in order to facilitate storage and handling, pharmaceutically acceptable carriers such as dextrin and cyclodextrin and any other auxiliaries can be added, and an extract from a plant belonging to the genus Acer can be added. It can be formulated into any dosage form such as powder, granules and tablets.

Since an extract from a plant belonging to the genus Acer has a unique odor, it is possible to carry out purification for the purpose of decolorization, deodorization, etc. within a range that does not reduce the physiological activity thereof. Since it is not used in a large amount when it is added to cosmetics or the like, there is no practical problem even if it is unpurified. Extracts from plants belonging to the genus Acer can be purified by, for example, treatment with activated carbon, adsorption resin treatment, ion exchange resin treatment, or the like.

The extract obtained from the plants belonging to the genus Acer, as described above, has a moisturizing action, an active oxygen eliminating action,
Radical scavenging action, collagen production promoting action, collagenase inhibitory action, fibroblast proliferation action, elastase inhibitory action, estrogen-like action, hyaluronidase inhibitory action, cyclic AMP phosphodiesterase inhibitory action or histamine release inhibitory action, so the extract It can be used as an active ingredient of moisturizers, antioxidants, anti-aging agents or anti-inflammatory agents, as well as active oxygen scavengers, radical scavengers, collagen production promoters, collagenase inhibitors, fibroblast proliferation agents, elastase inhibition It can be used as an active ingredient of an agent, an estrogen-like agent, a hyaluronidase inhibitor, a cyclic AMP phosphodiesterase inhibitor or a histamine release inhibitor. When applying the various agents of the present invention to the skin, the moisturizing agent of the present invention may be directly applied to the skin, or the various agents of the present invention may be blended in a skin cosmetic and applied to the skin. .

The moisturizing agent of the present invention can prevent and / or improve various skin diseases such as rough skin through its moisturizing effect on the skin.

The antioxidant of the present invention eliminates active oxygen and in-vivo radicals through active oxygen scavenging action and / or radical scavenging action to prevent and / or ameliorate skin aging and various skin diseases caused thereby. can do. Here, "active oxygen" includes superoxide, hydrogen peroxide, hydroxy radicals, singlet oxygen, and the like.
Further, the “radical” means a molecule or atom having one or more unpaired electrons, and includes superoxide, hydroxy radical, DPPH and the like.

The anti-aging agent of the present invention can be used to stimulate the skin through one or more actions selected from the group consisting of collagen production promoting action, collagenase inhibiting action, fibroblast proliferation action, elastase inhibiting action and estrogen-like action. And / or prevent various skin diseases caused by aging
Or it can be improved.

The anti-inflammatory agent of the present invention has a contact dermatitis (rash) through one or more actions selected from the group consisting of a hyaluronidase inhibitory action, a cyclic AMP phosphodiesterase inhibitory action and a histamine release inhibitory action. Various inflammatory diseases including skin diseases such as psoriasis and pemphigus vulgaris can be prevented and / or ameliorated.

The extract from a plant belonging to the genus Acer has a moisturizing action, an antioxidant action, an anti-aging action or an anti-inflammatory action, and is excellent in the feeling of use and safety when applied to the skin. It is suitable for incorporation into cosmetics.
By adding an extract from a plant belonging to the genus Maple to a skin cosmetic, it is possible to impart a moisturizing action, an antioxidant action, an anti-aging action or an anti-inflammatory action to the skin cosmetic.

The skin cosmetic containing an extract from a plant belonging to the genus Maple is blended with any main agent or auxiliary agent which does not impair the physiological activity of the extract from the plant belonging to the genus Acer. The extract may be the main component.

The kind of skin cosmetics to which the extract from the plant belonging to the genus Acer can be blended is not particularly limited, and specific examples thereof include ointments, creams, emulsions, lotions, packs, foundations and the like. To be

The amount of the extract from a plant belonging to the genus Maple in the skin cosmetics can be appropriately adjusted depending on the kind of the skin cosmetics, but it is usually 0.00
It is from 01 to 10% by weight, preferably from 0.001 to 1% by weight. If the blending amount is less than 0.0001% by weight, it is difficult to obtain a sufficient effect, and if the blending amount exceeds 10% by weight, the storage stability decreases.

The skin cosmetic of the present invention may optionally contain any medicinally active ingredient such as a whitening agent, an anti-inflammatory agent or a physiologically active substance. Suitable whitening agents to be added to skin cosmetics include, for example, one or more selected from the group consisting of ascorbic acid or a derivative thereof, placenta extract, kojic acid, rucinol, ellagic acid and chamomile extract. Examples include whitening agents. Further, as an anti-inflammatory agent suitable for incorporation into skin cosmetics, for example, allantoin, guaiazulene or a derivative thereof, glycyrrhizic acid or a salt or derivative thereof, glycyrrhetinic acid or a salt or derivative thereof, epsilon aminocaproic acid or a derivative thereof, Tranexamic acid, ibuprofen, indomethacin, zinc oxide, arnica extract, Indian ginkgo extract, eugon extract, licorice extract, peony extract, sycamore extract, birch extract, horse chestnut extract, mukuroji extract and rosemary extract Examples include one or more anti-inflammatory agents selected from the group consisting of products.

The skin cosmetics of the present invention which can be used as a constituent together with an extract from a plant belonging to the genus Acer are shown below. When the following constituents are used in combination, the synergistic action between the extract from a plant belonging to the genus Acer and the constituents used in combination may bring about an excellent use effect more than normally expected.

Astringents: citric acid or salts thereof, tartaric acid or salts thereof, lactic acid or salts thereof, aluminum chloride, potassium aluminum sulfate, allantoin dihydroxyaluminum, zinc sulfate, proanthocyanidins, guerrilla extract, rhubarb extract, cucumber extract, cucumber extract. , Melissa extract, etc.

Bactericidal / antibacterial agents: benzoic acid, sodium benzoate, paraoxybenzoic acid ester, distearylmethylammonium chloride, orthophenylphenol, photosensitizer 101, photosensitizer 201, salicylic acid, sodium salicylate, sorbic acid, resorcin, Phenoxyethanol, bisabolol, hinokitiol, oil-soluble licorice extract (liquorice hydrophobic flavonoid, glabridin, glabrene, lycochalcone A) and the like.

UV absorber: β-isopropylfuranone derivative, urocanic acid, ethyl urocanate, oxybenzone, octyl paradimethyl benzoate, octyl paraaminobenzoate, paraaminobenzoic acid, ethyl paraaminobenzoate, butylmethoxydibenzoylmethane, titanium oxide , Β-carotene and the like.

Moisturizers: serine, glycine, threonine,
Alanine, sodium pyrrolidonecarboxylate, collagen, hydrolyzed collagen, vitronectin, fibronectin, keratin, elastin, royal jelly, chondroitin heparin, glycerophosphate lipid, lactic acid fermented product, yeast extract, soybean phospholipid, γ-oryzanol,
Boiled Aoi extract, Yokuinin extract, etc.

Cell enhancer: riboflavin or its derivative, pyridoxine or its derivative, nicotinic acid or its derivative, pantothenic acid or its derivative, α-tocopherol or its derivative, Yukinoshita extract, garlic extract, yeast extract, carrot extract, mannen wax extract. Such.

Anti-inflammatory / anti-allergic agents: azulene, allantoin, aminocaproic acid, indomethacin, lysozyme chloride, glycyrrhizic acid or its derivative, glycyrrhetinic acid or its derivative, Photosensitizer No. 301, Photosensitizer No. 401, diphenhydramine hydrochloride, adenosine phosphate, Estradiol, Eslon, ethinyl estradiol, Coptis extract, Perilla extract, Scutellaria extract, etc.

Antioxidant / active oxygen scavenger: dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate, baicalin, baicalein, superoxide dismutase, catalase, rosemary extract, nutmeg extract, mace extract, laurel extract,
Turmeric extract, licorice flavonoid, etc.

Fats and oils: soybean oil, linseed oil, sesame oil, rapeseed oil, safflower oil, olive oil, camellia oil, almond oil, castor oil, coconut oil, beef tallow, jojoba oil, evening primrose oil and the like.

Waxes: carnauba wax, candelilla wax, beeswax, honey beeswax, spermaceti, cerax, lanolin and the like.

Hydrocarbons: liquid paraffin, petrolatum,
Micro listerin wax, ceresin, squalane, polyethylene powder, etc.

Fatty acids: stearic acid, linoleic acid, lauric acid, myristic acid, palmitic acid, hevenic acid,
Lanolin acid, oleic acid, etc.

Alcohols: lauryl alcohol, cetyl alcohol, stearyl alcohol, lanolin alcohol, oleyl alcohol, hexadecyl alcohol,
Propylene glycol, 1,3-butylene glycol, ethylene glycol or its polymer, cetostearyl alcohol, etc.

Esters: Decyl oleate, butyl stearate, myristyl myristate, glyceryl monostearate, glyceryl trimyristate, lanolin acetate, cetyl lactate and the like.

Surfactants: Anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, natural elementary surfactants such as quillaja saponin and the like.

Fragrances: menthol, carvone, eugenol, anethole, peppermint oil, spearmint oil, peppermint oil, eucalyptus oil, anise oil and other oily flavors from various plants and animals.

[0056]

The present invention will be described in more detail with reference to the following examples. [Production Example 1] 100 g of dried leaves of dried leaves of Acer palmatum (Acer palmatum) were added to 100 g of water, 50% ethanol (weight ratio of water to ethanol: 1: 1), and 1000 mL of ethanol, and the mixture was refluxed with an extractor. The mixture was extracted by heating at 80 ° C for 2 hours, and filtered while hot. The same extraction treatment was further performed on the residue. The obtained extracts were combined, concentrated under reduced pressure, and further dried to obtain an extract at each site. The yield of the extract was as shown in Table 1.

[0057]

[Table 1]

[Test Example 1] Moisturizing test: 0.05% aqueous solution of 50% ethanol extract of Japanese maple (sample solution 1), 1% glycerin (sample solution 2),
Purified water (sample solution 3) was added to a paper disk with a diameter of 8 mm (manufactured by Toyo Seisakusho, weight 0.017).
10 g each was added dropwise to g). Leave this in the test room,
The weight after 0 to 8 minutes was measured every 1 minute. The water residual rate (%) of each sample solution was determined with the weight of 0 minutes as 100%. The test was conducted at room temperature of 24 ° C. and humidity of 68%. 0
Table 2 shows the residual water content (%) of each sample solution after -8 minutes.
Shown in.

[0059]

[Table 2]

As shown in Table 2, the sample solution (sample solution 1) containing the extract of Japanese maple tree was purified water (sample solution 3) in the same manner as the sample solution (sample solution 2) containing glycerin (humectant). Water retention rate was higher than From this, it was confirmed that the Japanese maple extract has a moisturizing effect.

[Test Example 2] Superoxide scavenging action test (NBT method) The extract obtained in Production Example 1 was tested for the superoxide scavenging action by the following test method.

3 mM xanthine, 0.05 M Na ₂ CO
0.1 mL each of ₃ buffer solution (pH 10.2), ₃ mM EDTA, BSA solution, and 0.75 mM NBT was placed in a test tube, 0.1 mL of the sample solution was added thereto, and the mixture was allowed to stand at 25 ° C. for 10 minutes. Then, a xanthine oxidase solution was added, the mixture was rapidly stirred, and the mixture was allowed to stand at 25 ° C. for 20 minutes. Then add 0.1 mL of 6 mM copper chloride to stop the reaction,
The absorbance at a wavelength of 560 nm was measured. The absorbance measured at this time is referred to as "absorbance upon addition of enzyme solution and sample solution".

The same operation and measurement of absorbance were carried out without adding the enzyme solution. The absorbance measured at this time is referred to as "absorbance with no enzyme solution added and sample solution added". The same measurement was performed when distilled water was added without adding the sample solution. The absorbance measured at this time is referred to as "absorbance with and without addition of enzyme solution". further,
The same measurement was performed in the case where distilled water was added without adding the enzyme solution and without adding the sample solution. The absorbance measured at this time is referred to as "absorbance when enzyme solution is not added and sample solution is not added". Then, the superoxide scavenging rate (%) was calculated by the following formula.

Erasure rate (%) = {1- (AB) / (C-
D)} × 100

In the above formula, "A" is "absorbance when enzyme solution is added and sample solution is added", "B" is "absorbance when enzyme solution is not added and sample solution is added", and "C" is "enzyme solution added""Absorptance without addition of sample solution" and "D" represent "Absorptivity without addition of enzyme solution and without addition of sample solution".

The erasure rate was measured by gradually decreasing the sample concentration, and the sample concentration (ppm; μg / mL) at which the superoxide erasure rate was 50% was determined by the interpolation method. The results are shown in Table 3.

[0067]

[Table 3]

From the results shown in Table 3, it was confirmed that the Japanese maple tree extract has a superoxide scavenging action. The degree of this superoxide scavenging action is
It was confirmed that the concentration can be adjusted depending on the concentration of the extract.

[Test Example 3] Radical scavenging action test on DPPH The extract obtained in Production Example 1 was tested for radical scavenging action by using DPPH which is a very stable radical by the following test method.

3 mL of the sample solution was added to 3 mL of the 1.5 × 10 ⁻⁴ M DPPH ethanol solution, the container was immediately sealed and shaken, and the mixture was allowed to stand for 30 minutes. Then wavelength 520
The absorbance at nm was measured. As a control, the same operation was performed using a solvent in which the sample solution was dissolved instead of the sample solution, and the absorbance at a wavelength of 520 nm was measured. Further, as a blank, 3 mL of the sample solution was added to ethanol, and immediately thereafter, the absorbance at a wavelength of 520 nm was measured. The radical scavenging rate (%) was calculated by the following formula from each measured absorbance.

[0071] Radical scavenging rate (%) = {1- (BC) / A} × 100

In the above formula, "A" is "absorbance of control", "B" is "absorbance of sample solution added",
"C" represents "blank absorbance".

The scavenging rate was measured by gradually reducing the sample concentration, and the sample concentration (ppm; μg / mL) at which the scavenging rate of DPPH radical was 50% was determined by the interpolation method. The results are shown in Table 4.

[0074]

[Table 4]

From the results shown in Table 4, it was confirmed that the Japanese maple extract has a DPPH radical scavenging action. It was also confirmed that the degree of this DPPH radical scavenging action can be adjusted by the concentration of the extract.

[Test Example 4] Collagen production promoting action test The extract obtained in Production Example 1 was tested for collagen production promoting action by the following test method.

Human fibroblasts were treated with 10% FBS, 1% N
M containing EAA, 1 mmol / L sodium pyruvate
The cells were cultured in EM medium at 37 ° C. under 5% CO ₂ -95% air, and the cells were collected by trypsin treatment and 2 × 10 ⁵ cells were collected.
After adjusting to / mL, 100 μL was seeded in each well of the 96-well microplate. 37 ° C, 5% CO ₂ -95%
After overnight culture under air, the medium was added to the sample solution (25
ppm) containing 0.5% FBS-MEM medium (150 μm)
L), and cultured at 37 ° C. under 5% CO ₂ -95% air for 3 days. 90 μL of the culture supernatant was collected,
After transferring to an ELISA plate, collagen in the culture supernatant was quantified by the ELISA method using an anti-human collagen type 1 antibody. In the quantification, a calibration curve using human collagen type 1 as a standard was used. Then, the collagen production promotion rate (%) is 100% when the sample is not added.
% Was calculated. The results are shown in Table 5.

[0078]

[Table 5]

From the results shown in Table 5, it was confirmed that the Japanese maple extract has a collagen production promoting action.

Test Example 5 Collagenase Inhibitory Action Test The extract according to Production Example 1 was tested for collagenase inhibitory action by the following test method.

Sample solution (solvent: 20 mmol / L calcium chloride-containing 0.1 mol / L Tris-HCl buffer (pH 7.1): the same applies to the following buffers) 50 μ
L, collagenase solution 50 μL and substrate solution 400 μL
L was mixed and incubated at 37 ° C for 30 minutes. Then, the reaction was stopped with 1 mL of 25 mmol / L citric acid solution, and the mixture was extracted with 5 mL of ethyl acetate. With respect to the obtained extract, the absorbance at a wavelength of 320 nm (control solution: ethyl acetate) was measured. The absorbance measured at this time is called "absorbance at the time of enzyme addition and sample solution addition". The collagenase solution is Collagenase Type IV 5 from Sigma.
mg was dissolved in 1 mL of a buffer solution and diluted 50 times before use. The substrate solution contains BA in the above buffer solution.
CHEM Fenichemikalian AG Pz
-The peptide was dissolved and used at a concentration of 0.5 mol / L.

The same enzymatic reaction and absorbance measurement as above were carried out by adding a buffer solution in an amount equal to that of the sample solution instead of the sample solution. The absorbance measured at this time is called "absorbance when enzyme is not added and sample solution is not added". Further, the same enzymatic reaction and absorbance measurement as above were carried out by adding a buffer solution instead of the collagenase solution. The absorbance measured at this time is referred to as "absorbance when enzyme is not added and sample solution is added". Furthermore, the same enzyme reaction and absorbance measurement as above were performed by adding a buffer solution in an amount equal to that of the sample solution instead of the sample solution, and by adding a buffer solution instead of the collagenase solution. The absorbance measured at this time is called "absorbance without addition of enzyme and without addition of sample solution". Then, the collagenase inhibition rate (%) was calculated by the following formula.

Collagenase inhibition rate (%) = {1- (A-
B) / (C-D)} × 100

In the above formula, "A" is "absorbance when enzyme is added and sample solution is added", "B" is "absorbance when enzyme is not added and sample solution is added", and "C" is "enzyme added and sample solution is added""Absence without addition" and "D" represent "absence without addition of enzyme and without addition of sample solution".

The above-mentioned inhibition rate was measured by gradually decreasing the sample concentration, and the sample concentration (ppm; μg / mL) at which the activity of collagenase was inhibited by 50% was determined by the interpolation method. The results are shown in Table 6.

[0086]

[Table 6]

From the results shown in Table 6, it was confirmed that the Japanese maple extract has a collagenase inhibitory action. It was also confirmed that the degree of this collagenase inhibitory action can be adjusted by the concentration of the extract.

[Test Example 6] Elastase Inhibitory Action Test The extract obtained in Production Example 1 was tested for its elastase inhibitory action by the following test method.

Prepare a 96-well plate, add 50 μL of sample solution (solvent: DMSO + water) and 50 μL of elastase solution to 1 well, and further add 100 μL of substrate solution.
Was added and mixed. After incubating at 25 ° C for 15 minutes, the absorbance at a wavelength of 415 nm was measured. The absorbance measured at this time is called "absorbance at the time of enzyme addition and sample solution addition". The elastase solution was 5 mg of elastase Type III from Sigma and 0.2 mol of pH8.
/ L Tris hydrochloric acid buffer was dissolved in 1 mL and diluted 250 times before use. As a substrate solution, N-SUCCINYL-ALA-ALA-ALA-p of Sigma
A solution of NITROANILIDE dissolved in DMSO at a concentration of 45.14 mg / mL was diluted 100 times with the Tris-HCl buffer and used.

The same enzyme reaction and absorbance measurement as above were carried out by adding only the solvent in an amount equal to that of the sample solution instead of the sample solution. The absorbance measured at this time is called "absorbance when enzyme is not added and sample solution is not added". In addition, the same enzyme reaction and absorbance measurement as above were carried out by the same operation and measurement by adding a buffer solution instead of the elastase solution. The absorbance measured at this time is referred to as "absorbance when enzyme is not added and sample solution is added". Furthermore, the same enzymatic reaction and absorbance measurement as above,
The same operation and measurement were performed by adding only the same amount of solvent as the sample solution instead of the sample solution, and adding a buffer solution instead of the elastase solution. The absorbance measured at this time is called "absorbance without addition of enzyme and without addition of sample solution".
Then, the elastase inhibition rate (%) was calculated by the following formula.

Elastase inhibition rate (%) = {1- (A-
B) / (C-D)} × 100

In the above formula, "A" is "absorbance when enzyme is added and sample solution is added", "B" is "absorbance when enzyme is not added and sample solution is added", and "C" is "enzyme added and sample solution is added""Absence without addition" and "D" represent "absence without addition of enzyme and without addition of sample solution".

The above-described inhibition rate was measured by gradually reducing the sample concentration, and the sample concentration (ppm; μg / mL) at which the activity of elastase was inhibited by 50% was determined by the interpolation method. The results are shown in Table 7.

[0094]

[Table 7]

From the results shown in Table 7, it was confirmed that the Japanese maple extract has an elastase inhibitory action. It was also confirmed that the degree of this elastase inhibitory effect can be adjusted by the concentration of the extract.

Test Example 7 Estrogen-Like Action Test The method of Thomas et al. For examining the effect of the extract obtained in Production Example 1 on the growth of estrogen-dependent cells (In
In vitro cell.Dev.Biol. 28A, 595-602, 1992), and the estrogenic effect was tested.

Human breast cancer-derived MCF-7 cells were treated with 75c
The cells were cultured in an m ² flask until they became confluent, and the MCF-7 cells were collected by trypsin treatment and collected at 10%.
Using MEM medium containing FBS (treated with activated carbon), 1% NEAA and 1 mM sodium pyruvate and not containing phenol red (hereinafter abbreviated as "MEM medium"),
It was adjusted to 3 × 10 ⁴ cells / mL. Prepared MCF-
0.9 mL of each of 7 cells was seeded on a 24-well plate, and at 37 ° C., 5% CO ₂ -95% air was added to fix the cells.
Cultured under After 6 hours (day 0), 100 μL of a sample solution prepared in MEM medium at a concentration 10 times the final concentration (12.5 ppm) was added to the plate, and the culture was continued. 6th day after the start of culture, the medium was 0.97 mmol / L
The medium was replaced with MEM medium containing MTT, and after culturing for 2 hours, the medium was replaced with isopropanol to extract blue formazan produced in the cells. Regarding the eluted isopropanol containing blue formazan, the absorbance at 570 nm at which the absorption maximum of blue formazan is present was measured.

In order to correct the influence of adherent cells, the absorbance at 650 nm was also measured at the same time, and the difference between the two absorbances was taken as a value proportional to the amount of blue formazan produced (the absorbance in the following formula is the corrected absorbance. Is). As a positive control, 0.02 ppm ethinyl estradiol was used. The strength of the estrogen-like action (estrogen-dependent proliferative action) was calculated based on the following equation, with the absorbance at the time when no sample was added as 100%. The test results are shown in Table 8.

[0099] Estrogen-like action (%) = A / B x 100

In the above formula, "A" represents the absorbance when the sample was added, and "B" represents the absorbance when the sample was not added.

[0101]

[Table 8]

As shown in Table 8, it was confirmed that the Japanese maple extract has an estrogen-like action.

[Test Example 8] Cell death inhibition test by UV irradiation [0103] The extract obtained in Production Example 1 was tested for cell death inhibition by UV irradiation by the following test method. Normal human fibroblasts were seeded in a 48-well plate and incubated at 37 ° C for 5
After culturing for 24 hours under% CO ₂ -95% air,
The medium was replaced with PBS (-) and 5.0 J / cm ² UV was added.
-B (302 nm) was irradiated. PBS immediately after irradiation
Discard the (-), replace the sample with a dissolved medium, and
Cultured for 4 hours. After culturing, the medium was exchanged with a 0.4 mg / ml MTT solution prepared in PBS (-), and after culturing for 2 hours, the medium was exchanged with isopropanol to extract blue formazan produced in the cells. Regarding the eluted isopropanol containing blue formazan, the absorbance at 570 nm, which has the absorption maximum of blue formazan, was measured. At the same time, to correct the effect of adherent cells, 65
The absorbance at 0 nm was also measured, and the difference between the two absorbances was used as a value proportional to the amount of blue formazan produced (the absorbance in the following calculation formula is the corrected absorbance). Also,
At the same time, cells that were not seeded with UV light after cell seeding and cells that had been seeded with UV light and had no sample added were also measured, and were taken as the non-irradiation group and the irradiation group, respectively. The recovery rate (%) from ultraviolet irradiation damage was calculated from the following formula, and it was judged that the larger this value, the greater the cell death suppressing effect by ultraviolet irradiation. The test results are shown in Table 9.

Recovery rate (%) = {{(A−B) − (A−
C)} / (AB)} × 100

In the above formula, "A" is "absorbance in cells that are not irradiated with ultraviolet rays", "B" is "absorbance in cells that are irradiated with ultraviolet rays and no sample is added", and "C" is "absorbance with ultraviolet rays". Absorbance in cells to which the sample is added ”. The test results are shown in Table 9.

[0106]

[Table 9]

As shown in Table 9, it was confirmed that the Japanese maple extract has an action of suppressing cell death of fibroblasts by ultraviolet irradiation and efficiently proliferating fibroblasts.

[Test Example 9] Hyaluronidase Inhibitory Action Test The extract obtained in Production Example 1 was tested for a hyaluronidase inhibitory action by the following test method.

Hyaluronidase solution (400 units /
(mL, pH 3.5 acetate buffer) 0.1 mL and sample solution 0.2 mL were mixed and incubated at 37 ° C. for 20 minutes, and then an activator solution (2.5 mM-CaCl ₂ )
0.2 mL was added and the enzyme was activated by incubation at 37 ° C. for 20 minutes. After adding 0.5 mL of potassium hyaluronate buffer and incubating at 37 ° C. for 40 minutes, 0.4 N sodium hydroxide (0.2 ml) was added and ice-cooled to stop the reaction. Then 0.8M
A boric acid solution (pH 9.1) (0.2 mL) was added, and the mixture was heated in a boiling bath for 3 minutes and immediately ice-cooled for 20 minutes. p-DAB
Add 6.0 mL of reagent A (dissolve 10 g of p-dimethylaminobenzaldehyde in 12.5 mL of 10N hydrochloric acid and 87.5 mL of acetic acid and dilute 10 times with acetic acid) and incubate at 37 ° C. for 20 minutes. The N-acetylglucosamine liberated by the above-mentioned enzymatic reaction was developed by the above, and the absorbance at a wavelength of 585 nm was measured. The absorbance measured at this time is hereinafter referred to as "absorbance upon addition of enzyme and addition of sample solution".

The same operation and absorbance measurement were carried out without adding the enzyme. The absorbance measured at this time is hereinafter referred to as "absorbance with no enzyme added and sample solution added". The same measurement was performed when distilled water was added without adding the sample solution. The absorbance measured at this time is hereinafter referred to as "absorbance when enzyme is not added and sample solution is not added". Furthermore, the same measurement was performed in the case where distilled water was added without adding the enzyme and without adding the sample solution. The absorbance measured at this time is hereinafter referred to as "absorbance without addition of enzyme and without addition of sample solution". Then, the hyaluronidase inhibition rate (%) was determined by the following formula.

Hyaluronidase inhibition rate (%) = {1-
(A−B) / (C−D)} × 100

In the above formula, "A" is "absorbance when enzyme is added and sample solution is added", "B" is "absorbance when enzyme is not added and sample solution is added", and "C" is "enzyme added and sample solution is added""Absence without addition" and "D" represent "absence without addition of enzyme and without addition of sample solution".

The above inhibition rate was measured by gradually reducing the sample concentration, and the sample concentration IC _{50 at} which the inhibition rate became 50% was obtained.
(ppm; μg / mL) was determined by the interpolation method. The results are shown in Table 10.

[0114]

[Table 10]

As shown in Table 10, it was confirmed that the Japanese maple extract has a hyaluronidase inhibitory action. It was also confirmed that the degree of this hyaluronidase inhibitory effect can be adjusted by the concentration of the extract.

[Test Example 10] Cyclic AMP phosphodiesterase inhibitory activity test The extract obtained in Production Example 1 was tested for cyclic AMP phosphodiesterase inhibitory activity by the following test method.

To 0.2 mL of Tris-HCl buffer (pH 7.5) containing 5 mM magnesium chloride, 0.1 mL of fetal serum alpmin solution and 0.1 mL of cyclic AMP phosphodiesterase solution were added, and 0.05 mL of sample solution was added. Pre-incubated at 37 ° C for 5 minutes. Then cyclic AMP solution 0.05m
L was added and incubated at 37 ° C. for 60 minutes.
Stop the reaction by boiling for 3 minutes in a boiling bath,
The reaction substrate 5'-AMP in the supernatant was centrifuged at 00 rpm.
Was quantified by high performance liquid chromatography. The same enzymatic reaction and reaction substrate analysis are performed without adding the sample solution, and the cyclic AMP phosphodiesterase inhibition rate (%) of the sample is determined from the ratio of the reaction group mass when the sample is added to the reaction group mass when the sample is not added. I asked.

The above-mentioned measurement was repeated by gradually decreasing the sample concentration of the sample solution, and the sample concentration IC at which the activity of cyclic AMP phosphodiesterase was inhibited by 50%
₅₀ (ppm; μg / mL) was determined by the interpolation method. The results are shown in Table 11.

[0119]

[Table 11]

From the results shown in Table 11, it was confirmed that the Japanese maple extract has a cyclic AMP phosphodiesterase inhibitory action. Also, this cyclic A
It was confirmed that the degree of MP phosphodiesterase inhibitory effect can be adjusted by the concentration of the extract.

[Test Example 11] Histamine release inhibitory effect test The extract obtained in Production Example 1 was tested for histamine release inhibitory effect by the following test method. In addition, the following test method is a test method that evaluates histamine release inhibitory activity using hexosaminidase release as an index, by utilizing the fact that intracellular histamine is released and hexosaminidase is released at the same time. is there.

25 cm^TwoMedium in culture flask
RB in (S-MEM medium containing 15% FBS; the same applies below)
L-2H3 cell 1.0 × 10⁶Seed, 37 ° C, 5
% CO _TwoCultured under -95% air for 4 days. Then
Treat with trypsin and centrifuge (800 rpm, 4 minutes)
Then the cells were collected. The cells obtained were 4.0 × 10⁵ce
It is suspended in the medium at 11 / mL and the mouse monoclonal
Anti-dinitrophenyl group IgE (DNP-Specif
ic IgE) was added at a concentration of 0.5 μg / mL.
Add 100μ of this cell suspension to each well of a 96-well plate.
L seeds at 37 ° C, 5% CO_Two-95% below air
The cells were cultured for 24 hours. After completion of culture, remove the medium in each hole
And washed twice with Silaghanian buffer. Next, the above buffer
Add 30 μL of the solution and 10 μL of the sample solution, and add 1 at 37 ° C.
Incubated for 0 minutes. Then dinitrophenyl
Add 10 μL of modified bovine serum albumin (DNP-BSA)
And further incubated at 37 ° C for 15 minutes. So
After that, add 10 μL of the supernatant to a new 96-well plate under ice cooling.
Transferred to this, 1 mmol / L p-nitrophenyl
10 μL of -N-acetyl-β-D-glucosamide solution
In addition, it incubated at 37 degreeC for 1 hour. End of reaction
After completion, 0.1 mol / L Na_TwoCO_Three-Na _TwoHCO
_ThreeAdd 250 μL of solution and place in a microplate reader.
Absorbance measurement at 415 nm against 650 nm
The measurement was performed (the absorbance measured at this time is A).

Further, the same treatment and absorbance measurement were carried out on the cell supernatant to which the silaganian buffer solution was added instead of the sample solution (the absorbance measured at this time is B).

In addition, the cell supernatant and 0.1 mol / L Na
On ₂ CO ₃ -Na ₂ HCO ₃ solution obtained by reacting the same processing is also performed absorbance measurement (absorbance measured this time is C).

In addition, the same processing and absorbance measurement were performed using DNP-
The experiment was also carried out with the addition of a Silaghanian buffer solution instead of BSA (the absorbance measured at this time is defined as D). And the hexosaminidase release inhibition rate (%) according to the following formula
I asked.

Release inhibition rate (%) = [1-{(A-C-
D) / (BD)}] × 100

The release inhibition rate was measured by gradually decreasing the sample concentration, and the release of hexosaminidase was 50%.
The inhibitory sample concentration (ppm; μg / mL) was determined by interpolation. The results are shown in Table 12.

[0128]

[Table 12]

From the results shown in Table 12, it was confirmed that the Japanese maple extract has a histamine release inhibitory action. It was also confirmed that the degree of histamine release inhibitory effect can be adjusted by the concentration of the extract.

[Examples 1 to 5 and Comparative Example 1] Lotions (Examples 1 to 5 and Comparative Example 1) having the compounding formulations (% by weight) shown in Table 13 were produced by a conventional method, and the following tests were conducted. It was

Twenty women in their 20s to 40s were divided into five groups of four, and after washing the face twice a day in the morning and evening for two weeks, the example lotion was applied to the right face and the comparative lotion was applied to the left face. did. At that time, feel during use, stickiness after use, persistence of moisturizing,
The four items of the rough skin improving effect were evaluated.

The evaluation is carried out by a five-point evaluation of 1 to 5 points, and "5 points" is "very good" (the feeling during use is very good, there is no stickiness after use, and the moisturizing persistence is Very high, very good skin roughening effect), "4 points" is "good" (feels good at the time of use, almost no stickiness after use, high moisturizing durability, high rough skin improvement effect) , "3 points" is "normal" (feeling when using is normal,
There is not much stickiness after use, there is a slight moisturizing persistence, there is a slight improvement effect on rough skin), "2 points" is "a little bad" (feeling when using, a little sticky after use, Moisturizing does not last long enough, skin roughening does not improve much, "1" is "bad" (feels bad when used, has a lot of stickiness after use, no moisturizing persistence, improves skin roughening) It has no effect).
Table 13 shows the average of the evaluation points.

[0133]

[Table 13]

From the results shown in Table 13, the lotion containing the Japanese maple extract (containing an ascorbic acid derivative, kojic acid, rucinol, ellagic acid or chamomile extract as a whitening agent) has a good feel when used, It was confirmed that there was little stickiness after use, and that the moisturizing durability and skin roughening improvement effect were excellent.

[Formulation Example 1] An emulsion having the following composition was produced by a conventional method. Jojoba oil 4.0 g Placenta extract 0.1 g Olive oil 2.0 g Squalane 2.0 g Cetanol 2.0 g Glyceryl monostearate 2.0 g Polyoxyethylene cetyl ether (20 EO) 2.5 g Polyoxyethylene sorbitan oleate ( 20E.O) 2.0 g 1,3-butylene glycol 3.0 g Hinokitiol 0.15 g Fragrance 0.05 g Japanese maple leaf extract 0.01 g Purified water balance (total amount is 100 g)

[Formulation Example 2] A cream having the following composition was produced by a conventional method. Liquid paraffin 5.0 g Salami beeswax 4.0 g Cetanol 3.0 g Squalane 10.0 g Lanolin 2.0 g Stearic acid 1.0 g Polyoxyethylene sorbitan oleate (20 EO) 1.5 g Glyceryl monostearate 3.0 g 1, 3-butylene glycol 6.0 g methyl paraoxybenzoate 1.5 g fragrance 0.1 g Japanese maple leaf extract 0.1 g purified water balance (total amount is 100 g)

[Formulation Example 3] A pack having the following composition was produced by a conventional method. Polyvinyl alcohol 15 g Polyethylene glycol 3 g Propylene glycol 7 g Ethanol 10 g Ethyl paraoxybenzoate 0.05 g Perfume 0.05 g Japanese maple leaf extract 0.05 g Purified water balance (total amount is 100 g)

[0138]

INDUSTRIAL APPLICABILITY The present invention provides a moisturizer, an antioxidant, an anti-aging agent and an anti-inflammatory agent, and a skin cosmetic having a moisturizing action, an antioxidant action or an anti-aging action. The moisturizers, antioxidants, anti-aging agents and anti-inflammatory agents and skin cosmetics provided by the present invention have excellent usability and safety on the skin, and are useful for preventing and improving rough skin.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61P 39/06 A61P 39/06 43/00 111 43/00 111 F term (reference) 4C083 AA071 AA111 AA112 AA122 AC022 AC072 AC102 AC122 AC132 AC182 AC242 AC302 AC311 AC312 AC422 AC442 AC482 AC841 AC842 AD042 AD112 AD512 AD552 AD641 AD642 BB47 CC02 CC04 CC05 CC07 EE06 EE07 EE12 EE16 FF01 4C088 AB12 AC05 BA08 MA02 MA07 MA63 ZA89 ZB21 ZC20

Claims (15)

[Claims] 1. A moisturizer, which comprises an extract from a plant belonging to the genus Acer, as an active ingredient. 2. The plant belonging to the genus Acer palmatum is Acer palmatum (Acer palmatum).
Moisturizer as described. 3. An antioxidant comprising an extract from a plant belonging to the genus Maple as an active ingredient. 4. The plant belonging to the genus Maple is Acer palmatum (Acer palmatum).
The antioxidant described. 5. The extract contains active oxygen scavenging activity and / or
Alternatively, it has a radical scavenging action, and the antioxidant according to claim 3 or 4. 6. An anti-aging agent, which comprises an extract from a plant belonging to the genus Acer, as an active ingredient. 7. The plant belonging to the genus Acer palmatum is Acer palmatum (Acer palmatum).
The described anti-aging agent. 8. The extract has one or more actions selected from the group consisting of a collagen production promoting action, a collagenase inhibiting action, a fibroblast proliferation action, an elastase inhibiting action and an estrogen-like action. The anti-aging agent according to claim 6 or 7, which is characterized. 9. An anti-inflammatory agent comprising an extract from a plant belonging to the genus Acer, as an active ingredient. 10. The anti-inflammatory agent according to claim 9, wherein the plant belonging to the genus Acer is Acer palmatum. 11. The extract is selected from the group consisting of a hyaluronidase inhibitory action, a cyclic AMP phosphodiesterase inhibitory action and a histamine release inhibitory action.
11. The anti-inflammatory agent according to claim 9 or 10, which has one or more actions. 12. A skin cosmetic, which comprises an extract from a plant belonging to the genus Acer. 13. The skin cosmetic according to claim 12, wherein the plant belonging to the genus Acer palmatum is Acer palmatum. 14. The skin cosmetic according to claim 12, further comprising a whitening agent. 15. The whitening agent is ascorbic acid or a derivative thereof, placenta extract, kojic acid, rucinol,
1 selected from the group consisting of ellagic acid and chamomile extract
15. The skin cosmetic according to claim 14, wherein the skin cosmetic is one kind or two or more kinds of whitening agents.