JP2014169256A - Anti-oxidant containing liquidambar formosana extract - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an anti-oxidant useful for an anti-aging, hair restoration, and the like, an in vivo anti-oxidant, and an intracellular oxidative stress inhibitor.SOLUTION: There is provided a composition which contains, as an active ingredient, an extract obtained from stems and/or leaves of Liquidambar formosana using water, a hydrophilic organic solvent, or a mixture thereof as an extracting solvent. The composition further contains ascorbic acid or a derivative thereof and/or tocopherol or a derivative thereof.

Description

  The present invention relates to an antioxidant, particularly an antioxidant effective in vivo, comprising an extract of a genus Fusarium as an active ingredient. The present invention is useful in the cosmetics manufacturing field and the like.

  Fu (Squirrel, scientific name: Liquidumbar formosana) is a deciduous tree of the genus Fu (family witch hazel in the Clonquist system). Sangakubafu (triangle leaf moth), Taiwanfu (Taiwan moth), Ika maple (Iga moth), Kamoka maple (Kamo niece), Okatsura (Male katsura). The dried fruit of mature fu is used as a herbal medicine, such as rorotsu (rochi-dori), for allergic diseases, bruise sprains and the like.

  Patent Document 1 discloses a human excess response inhibitor containing, as active ingredients, nucleotides and / or physiologically acceptable salts thereof and one or more active blood agents selected from natural products including rorotsu. Disclosed is a therapeutic agent for allergic diseases. Patent Document 2 discloses a lipase inhibitor containing one or more water extracts of plants selected from the group containing fu or an alcohol extract of plants selected from them. Further, Patent Document 3 discloses a dopa oxidase activity inhibitor, melanin production inhibitor containing rorotsu or an organic solvent extract thereof as an active ingredient, and a whitening agent containing rorotsu or an organic solvent extract thereof as an active ingredient.

  On the other hand, cell oxidation is known as one of the causes of skin aging. In addition, attempts have been made to use components having an antioxidant effect for hair restoration (Patent Documents 4 and 5).

JP-A-8-40921 JP2005-8572 JP2012-149022 JP-A-11-246361 International Publication WO2009 / 099167

  The present inventors have studied a plant-derived component having an antioxidant action. In particular, it has been intensively studied on components that effectively reduce oxidative stress in vivo. As a result, the present inventors have found that a fu extract has an excellent antioxidant ability and completed the present invention.

The present invention provides the following.
[1] An antioxidant comprising an extract of a plant of the genus Fusarium as an active ingredient.
[2] An in vivo antioxidant comprising an extract of a plant of the genus Fusarium as an active ingredient.
[3] An intracellular oxidative stress inhibitor comprising an extract of a genus Fusarium as an active ingredient.
[4] The agent according to any one of [1] to [3], wherein the plant of the genus Fuhu is Fumu (Liquidambar formosana).
[5] The agent according to any one of [1] to [4], wherein the extract is an extract of a stem and / or leaf of a plant belonging to the genus Fusarium.
[6] The agent according to any one of [1] to [5], wherein the extract is obtained using water, a hydrophilic organic solvent, or a mixture thereof as a solvent.
[7] The agent according to any one of [1] to [6], further comprising ascorbic acid or a derivative thereof and / or tocopherol or a derivative thereof.
[8] The agent according to any one of [1] to [7], which is added to a skin external preparation or cosmetic for anti-aging.
[9] The agent according to any one of [1] to [8], which is added to a skin external preparation or cosmetic used for hair styling, hair nourishing, or hair tonic.
[10] A cosmetic method comprising a step of applying an extract of a plant of the genus Fusarium to the skin to suppress oxidative stress in the stratum corneum, epidermis or dermis.

FIG. 1 is a graph comparing the effects of the extracts of Production Example 1, Production Example 2 and Comparative Example 1.

  Hereinafter, the present invention will be described in detail. In the present specification, “to” means a range including numerical values before and after. In addition, numerical values expressed in% are values based on mass, unless otherwise specified.

  The antioxidant or intracellular oxidative stress inhibitor of the present invention (hereinafter sometimes referred to as “the agent of the present invention”) is an effective extract from a plant belonging to the genus Fusarae (Mansacaceae in the Clonquist family). Ingredients.

  The plant of the genus Fusarium used in the present invention is not limited to a species, except for the case described in particular, and may be any species. Plants of the genus Fuzhou include Fu (Liquidambar formosana), Momijibafu (Liquidambar styraciflua), Liquidambar acalycina, and Stylax (Liquidambar orientalis). In the present invention, preferably Fu (Liquidambar formosana) is used as a plant belonging to the genus Fusarium.

  The part of the plant of the genus Fusarium used in the present invention refers to the whole plant or a part thereof, unless otherwise specified. “Part” includes trunk (including bark and xylem), leaf (including leaf blade, petiole, and bamboo leaf), root, flower (including petal and sepal), persimmon, fruit, and seed. included. In the present invention, bark, xylem, leaves, or a mixed part thereof, more preferably a trunk or a leaf, is preferably used as the Fucus plant. In the present invention, a part of the plant belonging to the genus Fusara excluding Lorotsu may be used.

  The plant of the genus Fusarium as the extraction raw material of the present invention may be raw or dried. Before being subjected to the extraction step, pretreatment such as cutting and pulverization may be performed so that extraction is performed efficiently.

  In the present invention, the term “extract” refers to a liquid obtained by immersing a raw plant belonging to the genus Fusarium in an extraction solvent, unless otherwise specified. , Concentrated, dried, etc. The form of the extract can be any of liquid, paste, gel, solid, powder and the like. Moreover, the pressing juice itself of the genus Fusarium may be sufficient, and what was concentrated, dried, etc. may be sufficient as this pressing juice. In order to obtain a solid or powder form, the liquid can be subjected to a drying process, and may be evaporated to dryness to form a solid, or may be dried by spray drying to form a powder, or may be freeze-dried. It may be a lyophilized product.

  The extraction solvent for obtaining the extract is not particularly limited. For example, water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), polyhydric alcohols (glycerol, propylene glycol, 1,3-butylene glycol (BG), etc. ), Ketones (acetone, methyl ketone, etc.), ethers (diethyl ether, tetrahydrofuran, etc.), esters such as ethyl acetate; one or more selected from the group consisting of hexane and the like can be used. Preferably, an aqueous solvent, specifically water, a hydrophilic organic solvent (for example, methanol, ethanol, glycerol, 1,3-butylene glycol), or a mixture thereof is used as the extraction solvent. When using a mixed solvent, the ratio is not particularly limited. Examples of the solvent that can be suitably used in the present invention are water or a mixed solvent of water and one or more selected from the group consisting of ethanol, glycerol, and 1,3-butylene glycol. : The mass ratio of water is 1 to 99:99 to 1, preferably 10 to 90:90 to 10, more preferably 30 to 70:70 to 30, for example 50:50. It can be purified by ion exchange resin treatment or the like.

  The extraction operation can be carried out by immersing the raw plant species in a solvent at room temperature or under heating. The ratio of the raw material genus plant and the solvent can be appropriately designed by those skilled in the art, for example, the amount of the solvent relative to 1 part by mass of the genus Fusarium plant material may be an amount that the raw material can be sufficiently immersed, The amount is preferably 0.5 parts by mass or more, more preferably 1 part by mass or more, and more preferably 2 parts by mass or more. The upper limit of the amount of solvent can also be determined from an economic point of view, as the amount of solvent relative to 1 part by weight of the raw material, for example, can be 100 parts by weight or less, preferably 50 parts by weight or less, 20 parts by weight More preferably, it is as follows. The extraction temperature and period can also be appropriately designed by those skilled in the art, and are carried out at room temperature or under heating for 1 hour to several months, preferably 6 hours to 1 week, more preferably 12 hours to 3 days. it can. Extraction may also depend on the method of steam distillation. When the extract is pressed juice, it can be prepared by a general method. For example, the washed raw material is crushed by adjusting the pH as necessary, and the obtained crushed material is subjected to an enzyme treatment if necessary to remove solids.

  Plant extracts may have a characteristic odor and color. Moreover, the stability may be poor, for example, odor or discoloration may occur over time or precipitation may occur. The extract may be purified for the purpose of decolorization, deodorization and the like within a range that does not cause a decrease in the target activity. As a method therefor, there are, for example, activated carbon treatment, adsorption resin treatment, and ion exchange resin treatment. In general, cosmetics are applied directly to the skin and hair, and remain applied for a relatively long time, and are used almost every day. Furthermore, the fragrance has a psychological and mental effect. However, the influence of the scent is very large, and the odor of the raw material components requires attention compared to products in other fields. Also pay attention to the appearance. According to the study by the present inventors, all the plant plant extracts produced in the examples have odors and colors suitable for use in cosmetics and can be expected to be stable as cosmetic ingredients. Met.

  In the present invention, an extract from a plant of the genus Fusarium is used as an active ingredient of an antioxidant (particularly an in vivo antioxidant) or an intracellular oxidative stress inhibitor. In the present invention, the term “in vivo” includes delivery destinations absorbed percutaneously, for example, between cells and cells. In the present invention, the term “cell” includes cells derived from skin (including scalp) unless otherwise specified.

A person skilled in the art can evaluate the presence or absence of an antioxidant ability or an ability to suppress intracellular oxidative stress by a method commonly used in this field. The ability to suppress intracellular stress is typically expressed as an intracellular stress elimination rate (%) or an IC ₅₀ value based on the method described in the implementation section of this specification, and can be compared and evaluated.

  According to the study by the present inventors, even if it is a component having antioxidant ability, in the case of an external preparation for skin and cosmetics, the intended effect cannot be exhibited unless it is percutaneously taken into the living body. If it is toxic to cells even when taken into the living body, the intended effect cannot be exerted, and it cannot be an active ingredient in a skin external preparation or cosmetic. Therefore, it is important to confirm the ability to suppress intracellular oxidative stress rather than confirming antioxidants by in vitro tests. The active ingredient of the present invention that has been confirmed to inhibit intracellular oxidative stress is an extracellular matrix (for example, fibrous proteins such as collagen and elastin; glycosamino such as hyaluronic acid (hyaluronan) and chondroitin sulfate). It can act to maintain the function of glycans and proteoglycans; cell adhesion proteins such as fibronectin and laminin). In addition, the active ingredient of the present invention can act for maintaining the function of a cell membrane, a tissue in a cell, and a gene in a cell nucleus in the cell itself and in the cell. In addition, regarding the intracellular oxidative stress in the present invention, when it is referred to as “suppression”, unless otherwise specified, it suppresses the generation of reactive oxygen species (ROS) in the cell and reduces the amount of generated ROS. including. Suppression of ROS generation or reduction of ROS amount may be the direct antioxidant action of the active ingredient, or the active ingredient activates intracellular antioxidant enzymes such as superoxide dismutase (SOD), catalase, glutathione peroxidase This can be caused by an indirect action.

  In the present invention, the term “agent” may be a Fusarium plant extract itself unless otherwise specified, and the extract may contain additives such as diluents, stabilizers, antioxidants, and preservatives. It may be composed of an extract and an additive to which is added. The agent of the present invention does not include existing skin external preparations and cosmetics.

  The agent of the present invention can contain, for example, 0.001% or more, preferably 0.01% or more, and preferably 0.1% or more of a Fusarium plant extract as a dry product obtained by the method shown in the examples of the present specification. More preferably, it is more preferably 1.0% or more. When an extract different from the examples is used, the design can be made in the same manner. When a liquid extract is used, a dry product obtained by the method shown in the examples of the present specification is used based on the solid content. You can design by calculating the amount to be done.

  The agent of the present invention can be used by adding to a skin external preparation or cosmetic. Examples of the external preparation for skin include those of the present invention that are particularly suitable for external use on the skin. Cosmetics include cosmetics and quasi-drugs, and also include basic cosmetics, makeup cosmetics, body cosmetics, hair cosmetics, scalp cosmetics, medicinal cosmetics, bath preparations, medicinal cosmetics, and hair restorers.

  When the agent of the present invention is added to a skin external preparation or cosmetic, the content thereof can be appropriately determined. For example, the dry matter obtained by the method shown in the examples of the present specification can contain 0.00001% or more, preferably 0.0001% or more, and more preferably 0.001% or more. . From the viewpoint that a higher effect can be maintained for a long period of time, the content is preferably 0.01% or more, and more preferably 0.1% or more. In any case, the upper limit value can be appropriately determined in consideration of economy, solubility, stability, color or odor resulting from the active ingredient, and the like. For example, it can be 20% or less, preferably 10% or less, and more preferably 5% or less. When an extract different from the examples is used, the design can be made in the same manner. When a liquid extract is used, it corresponds to the dried product obtained by the method shown in the examples of this specification based on the solid content. The amount to be calculated can be designed.

  In a preferred embodiment of the present invention, the Fusarium plant extract as an active ingredient is used in combination with ascorbic acid or a derivative thereof and / or tocopherol (d-α-tocopherol or dl-α-tocopherol) or a derivative thereof. . The combined use is to use together, and is typically to use both in one agent or composition, but each as a separate agent (or another composition), at the same time, Or use in order.

  In the present invention, “derivative” refers to a salt, ester, or glycoside that is acceptable as an external skin preparation or cosmetic, unless otherwise specified. It may be water-soluble or oil-soluble. Examples of ascorbic acid derivatives are ascorbyl 6-stearate, ascorbyl 6-palmitate, ascorbyl 2,6-dipalmitate, ascorbyl 2,3,5,6-tetrahexyldecanoate, ascorbyl / tocopheryl) phosphate K, ascorbine Acid-2-sulfuric acid 2Na, ascorbic acid Na, ascorbic acid-2-phosphate Mg, ascorbic acid-2-phosphate Na, ascorbyl-2-glucoside. Among them, ascorbic acid-2-phosphate Mg, ascorbic acid-2-phosphate Na and ascorbyl-2-glucoside are preferable, and ascorbyl-2-glucoside is particularly preferable.

  Examples of tocopherol derivatives are tocopherol acetate (d-α-tocopherol acetate or dl-α-tocopherol acetate), tocopherol nicotinate (d-α-tocopherol nicotinate, dl-α-tocopherol nicotinate), tocopheryl phosphate Na Ascorbyl / tocopheryl phosphate K, ascorbyl tocopherin maleate. Of these, tocopherol acetate and tocopherol nicotinate are preferable, and tocopherol acetate is particularly preferable.

  Those skilled in the art can appropriately set the blending amount and ratio of each component in the combined use. In the case of the combined use, the ratio of the extract of the genus plant to the combined component is 1 part by mass of the solid content of the genus plant extract (the amount corresponding to the dried product obtained by the method shown in the examples of the present specification). On the other hand, it may be 0.0025 to 25,000 parts by mass, preferably 0.025 to 2,500 parts by mass, and more preferably 0.25 to 250 parts by mass.

  The external preparation for skin or cosmetics of the present invention (these may be collectively referred to as “the composition of the present invention”) are variously acceptable as cosmetics or pharmaceuticals as long as the effects of the agent of the present invention are not impaired. These functional ingredients can be blended. Examples of such ingredients are whitening agents, UV protection agents, antibacterial agents, anti-inflammatory agents, cell activators, active oxygen scavengers, moisturizers, skin cleansing ingredients, acne and acemo prevention ingredients.

  In the composition of the present invention, various additives acceptable as cosmetics or pharmaceuticals can be blended in addition to the agent of the present invention within a range not impairing the effects of the present invention. Examples of this are water (purified water, hot spring water, deep sea water, etc.), surfactants (emulsifiers, solubilizers, suspending agents, stabilizers, etc.), antioxidants, preservatives, gelling agents, alcohol , Film forming agents, colorants, fragrances, deodorants, salts, pH adjusters, refreshing agents, chelating agents, keratolytic agents, enzymes, vitamins and the like.

  The composition of the present invention can also be prepared into compositions of various dosage forms such as a solid agent, a semisolid agent, and a liquid agent depending on the purpose of use. More specifically, the composition of the present invention can be used as a basic cosmetic, cleansing, facial cleanser, lotion, milk, cream, massage product, pack product, serum / gel, lip care product, etc .; , Face powder, makeup base, concealer, etc .; point makeup cosmetics, lipstick, lip gloss liner, teak products, eye shadow, eyeliner, mascara, eyebrow products, etc .; body cosmetics, soap, liquid detergent, sunscreen cream Bath cosmetics, etc .; shampoo, rinse, hair treatment, hair conditioner, hair tonic, hair restorer, scalp treatment, etc. as hair cosmetics or scalp cosmetics. Moreover, it can be set as a plaster, an ointment, a poultice, a liniment, a lotion, a coating agent, a patch, an aerosol (spray).

  The composition of the present invention can also be a kit or commercial package. These aspects may include, in addition to the composition of the present invention, a method (for example, a box, a container, a label, an instruction manual, and a tag) on which the method of use and the above-described effects and effects are described.

  The agent of the present invention or a skin external preparation or cosmetic to which the agent is added is used for treatment of a condition improved by antioxidation or by suppressing intracellular oxidative stress, for example, for anti-aging of skin, hair growth, and cosmetics. Can be used. The term “hair growth” in the present invention, unless otherwise specified, scalp for the purposes of hair loss and / or prevention / prevention of thinning hair, promotion of hair growth, hair growth, speeding hair growth, hair growth, and hair growth. And / or moisturizing the hair and keeping the skin healthy for hair growth purposes.

  The term “beauty (method)” in the present invention, unless otherwise specified, is to be performed only by medical personnel (medical personnel such as nurses and midwives who have received instructions from doctors and physicians under the Medical Doctor Law). Is not included.) In addition, the term “skin” or “skin” is not limited unless specifically described, and includes any skin on the body surface including the scalp. The method for anti-aging, the hair-growth method, and the beauty method of the present invention includes a case where the method is carried out on another person. The present invention also provides a method for producing an antioxidant, an intracellular oxidative stress inhibitor, or an external preparation for skin or a cosmetic, which comprises a step of adding and strengthening a Fusarium plant extract. In the present invention, the “adding step” of the genus plant extract to a cosmetic or the like includes using the plant genus plant extract as a raw material in the commercial production of the cosmetic or the like. The method of the present invention can be applied to a person other than a doctor (including a medical worker such as a nurse / midwife who has received an instruction from the doctor), such as a manufacturer or seller of cosmetics or pharmaceuticals, a makeup artist, or a beauty. It can be done by staff or estheticians.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated further more concretely, the scope of the present invention is not limited to the following Example.

[Production Example 1]
10 g of dried hum (Liquidambar formosana) trunk was cut into small pieces, immersed in 100 mL of methanol, and extracted with stirring for 24 hours. The extract was separated by filtration, concentrated using a rotary evaporator, and lyophilized to obtain an extract. The yield was 0.72 g (yield 7.2%).

[Production Example 2]
10 g of dried leaf (Liquidambar formosana) leaf was cut into small pieces, immersed in 100 mL of methanol and extracted for 24 hours with stirring. The extract was separated by filtration, concentrated using a rotary evaporator, and lyophilized to obtain an extract. The yield was 0.87 g (yield 8.7%).

[Production Example 3]
10 g of dried hum (Liquidambar formosana) trunk was cut into small pieces, immersed in 100 mL of 50% ethanol aqueous solution, and extracted for 24 hours with stirring. The extract was separated by filtration, concentrated using a rotary evaporator, and lyophilized to obtain an extract. The yield was 0.64 g (6.4% yield).

[Production Example 4]
10 g of dried fruit (Liquidambar formosana) fruit was chopped, soaked in 100 mL of 50% ethanol aqueous solution and extracted for 24 hours with stirring. The extract was separated by filtration, concentrated using a rotary evaporator, and lyophilized to obtain an extract. The yield was 1.0 g (yield 10.0%).

[Production Example 5]
10 g of dried dried leaf (Liquidambar styraciflua) leaves were cut into small pieces, immersed in 100 mL of 50% aqueous ethanol, and extracted for 24 hours with stirring. The extract was separated by filtration, concentrated using a rotary evaporator, and lyophilized to obtain an extract. The yield was 0.58 g (yield 5.8%).

[Comparative Example 1]
A pine genus extract with known antioxidant effect was used as a comparison target. A pine (Pinus thunbergii) trunk (10 g) was chopped and extracted according to the method of Production Example 1 to obtain an extract of Comparative Example 1. The yield was 0.91 g (yield 9.1%).

[Test method]
This method uses 2 ′, 7′-Dichlorofuorescein diacetate (DCFH-DA) that reacts with intracellular ROS and emits fluorescence, and refers to the method described in Non-Patent Document 1 below. DCFH-DA having high cell membrane permeability easily penetrates into cells, is deacetylated by intracellular esterase, and remains in the cells as non-fluorescent DCFH. Since DCFH is oxidized and converted into fluorescent DCF by intracellular ROS, the measured fluorescence intensity of DCF is an indicator of the amount of intracellular ROS. Although the amount of intracellular ROS is increased by hydrogen peroxide treatment, the intracellular ROS is increased by direct antioxidant action of the measurement sample or by indirect action caused by activation of the cell's own antioxidant enzymes by the measurement sample. Is erased and the amount of ROS decreases, it is observed as a decrease in fluorescence intensity compared to the control.

Non-patent document 1:
Kelly L. Wolfe, Rui Hai Liu, Cellular Antioxidant Activity (CAA) Assay for Assessing Antioxidants, Foods, and Dietary Supplements.J Agric Food Chem 2007, 55, p8896

[Test Example 1]
HepG2 cells were cultured in MEM medium containing 10% FBS at 37 ° C. in the presence of 5% CO ₂ for 1 week. One week later, cells were collected using a 0.125% trypsin-EDTA solution and seeded at a concentration of 3 × 10 ⁵ cells / cm ² in a 24-well plate. After culturing at 37 ° C. in the presence of 5% CO ₂ for 24 hours, each well was washed once with PBS (−), and 1 mL of fresh medium was added. Subsequently, the fux extracts of Production Examples 1 and 2 or Comparative Example 1 were dissolved in DMSO and added to a final concentration of 10 ug / mL. Furthermore, DCFH-DA (manufactured by Sigma-Aldrich Japan Co., Ltd.) was diluted with PBS (−) and added to a final concentration of 25 μM. After 1 hour of incubation at 37 ° C in the presence of 5% CO ₂ , hydrogen peroxide (Wako Pure Chemical Industries, Ltd.) was diluted with PBS (-) and added to a final concentration of 200 µM. Let stand for hours. After 1 hour, each well was washed with PBS (−), 1 mL of PBS (−) was added, and the fluorescence intensity at 538 nm was measured at an excitation wavelength of 485 nm. Intracellular oxidative stress elimination rate was calculated according to equation (1).

  The results are shown in the table below and FIG.

  Compared with the black pine extract of Comparative Example 1, the fux extracts of Production Examples 1 and 2 had a higher effect of eliminating intracellular oxidative stress and were shown to have an excellent antioxidant action.

[Test Example 2: IC ₅₀ ]
HepG2 cells were cultured in MEM medium containing 10% FBS at 37 ° C. in the presence of 5% CO ₂ for 1 week. One week later, cells were collected using a 0.125% trypsin-EDTA solution and seeded at a concentration of 3 × 10 ⁵ cells / cm ² in a 24-well plate. After culturing at 37 ° C. in the presence of 5% CO ₂ for 24 hours, each well was washed once with PBS (−), and 1 mL of fresh medium was added. Subsequently, the fuxed extract of Production Example 3 was dissolved in DMSO, and final concentrations were 50 ug / mL, 16.7 ug / mL, 5.6 ug / mL, 1.9 ug / mL, 0.6 ug / mL, 0.2 ug / mL, and 0.07 ug, respectively. / ML and 0.02ug / mL were added. Furthermore, DCFH-DA (manufactured by Sigma-Aldrich Japan Co., Ltd.) was diluted with PBS (−) and added to a final concentration of 25 μM. After 1 hour of incubation at 37 ° C. in the presence of 5% CO ₂ , hydrogen peroxide (Wako Pure Chemical Industries, Ltd.) was diluted with PBS (−) and added to a final concentration of 200 μM. Let stand for hours. After 1 hour, each well was washed with PBS (−), 1 mL of PBS (−) was added, and the fluorescence intensity at 538 nm was measured at an excitation wavelength of 485 nm. The intracellular oxidative stress extinction rate was calculated from the fluorescence intensity at each added concentration of the sample according to the formula (1), and then IC ₅₀ (sample concentration necessary for halving the intracellular ROS amount) was calculated and compared. Vitamin C and water-soluble vitamin E (Trolox: Sigma-Aldrich Japan Co., Ltd.) were used for comparison.

  The results are shown in the table below.

Production example 3 fu extract has a lower IC ₅₀ than vitamin C and vitamin E, which are widely used as antioxidants, and it is clear that it exhibits an antioxidant effect superior to vitamin C and vitamin E in cells Became.

[Prescription example]
[Lotion 1]
(Manufacturing method)
A. The following components (1) to (8) are mixed and dissolved.
B. The following components (9) to (15) are mixed and dissolved.
C. Add A to B and mix to obtain lotion.
(Component) (mass%)
(1) Meadow home oil 0.1
(2) Jojoba oil 0.05
(3) Perfume appropriate amount
(4) Phenoxyethanol 0.1
(5) Polyoxyethylene monooleate (20E.O.) sorbitan 0.5
(6) Polyoxyethylene isostearate (50E.O.) hydrogenated castor oil 1.0
(7) Ethanol 8.0
(8) Tocopherol acetate 0.05
(9) Dipotassium glycyrrhizinate 0.1
(10) Glycerin 5.0
(11) 1,3-butylene glycol 5.0
(12) Polyethylene glycol 1500 0.1
(13) Fuso extract (* 1) 0.1
(14) Hydrolyzed collagen 0.001
(15) Purified water remaining
(* 1) Prepared by Production Example 3

[Lotion 2]
(Manufacturing method)
A. The following components (1) to (9) are mixed and dissolved.
B. The following components (10) to (13) are mixed and dissolved.
C. Add B to A and mix to obtain lotion.
(Component) (mass%)
(1) Sodium hydroxide 0.3
(2) Citric acid 0.1
(3) Sodium monohydrogen phosphate 0.1
(4) Disodium edetate 0.05
(5) Ascorbic acid glucoside 2.0
(6) Fuso extract (* 2) 0.1
(7) Glycerin 3.0
(8) Dipropylene glycol 8.0
(9) Purified water remaining
(10) Ethanol 10.0
(11) Appropriate perfume
(12) Polyoxyethylene hydrogenated castor oil 0.05
(13) Tetra-2-ethylhexanoic acid pentaerythritol 0.2
(* 2) Prepared according to Production Example 4.

[Emulsion]
(Manufacturing method)
A. Heat a part of the following component (11) and keep it at 70 ° C.
B. The following components (1) to (10) are heated and mixed and maintained at 70 ° C.
C. Add A to B, mix, and uniformly emulsify.
After cooling D.C, the following components (12) to (16) and the remainder of (11) were mixed and then added and mixed uniformly to obtain an emulsion.
(Component) (mass%)
(1) Polyoxyethylene (10E.O.) sorbitan monostearate 1.0
(2) Polyoxyethylene (60E.O.) sorbitan trioleate 0.5
(3) Glyceryl monostearate 1.0
(4) Stearic acid 0.5
(5) Behenyl alcohol 0.5
(6) Squalane 8.0
(7) Ethanol 5.0
(8) Retinol palmitate 0.1
(9) Stearyl glycyrrhetinate 0.1
(10) Phenoxyethanol 0.1
(11) Remaining amount of purified water
(12) Carboxyvinyl polymer 0.2
(13) Sodium hydroxide 0.1
(14) Hyaluronic acid 0.1
(15) Fuso extract (* 3) 0.03
(16) Appropriate perfume
(* 3) Prepared according to Production Example 5.

[Liquid Foundation (oil-in-water emulsion)]
(Production method)
A. Disperse components (6) to (11).
B. Add components (12) to (17) to A and mix uniformly at 70 ° C.
C. Ingredients (1) to (5) are uniformly mixed at 70 ° C.
D. Add B to C, emulsify, and cool to room temperature.
Components (18) and (19) were added to ED and mixed uniformly to obtain an oil-in-water cream liquid foundation.
(Component) (mass%)
(1) Acrylic acid / alkyl methacrylate copolymer (Note 1) 0.5
(2) Triethanolamine 1.5
(3) Purified water remaining
(4) Glycerin 5
(5) Methyl paraoxybenzoate 0.1
(6) 1,3 Butylene glycol 5
(7) Hydrogenated soybean phospholipid 0.5
(8) Teflon (registered trademark) treated titanium oxide 5
(9) Octyl silylation treatment Bengala 0.1
(10) Silicone-treated yellow iron oxide 1
(11) Silicic acid-treated black iron oxide 0.05
(12) Stearic acid 0.9
(13) Glycerol monostearate0.3
(14) Cetostearyl alcohol 0.4
(15) Polyoxyethylene monooleate (20E.O.) sorbitan 0.2
(16) Polyoxyethylene trioleate (20E.O.) sorbitan 0.2
(17) 2-methoxyhexyl paramethoxycinnamate 5
(18) Fuso extract (* 4) 1
(19) Fragrance 0.02
(* 4) Prepared according to Production Example 3.
(Note 1) Pemulen TR-2 (NOVEON)

[Sunscreen cosmetic (water-in-oil cream)]
(Production method)
A. Disperse components (1) to (6) uniformly.
B. Disperse components (7) to (11) uniformly.
C. Disperse components (12) to (16) uniformly.
D. After uniformly mixing A and B, C was gradually added and emulsified to obtain a water-in-oil cream sunscreen cosmetic.
(Component) (mass%)
(1) Zinc oxide treated with silicon isopropyl triisostearoyl titanate 10
(2) Hydrogen dimethicone-treated fine particle titanium oxide 5.0
(3) Lauryl PEG-9 polydimethylsiloxyethyl dimethicone 3.5
(4) PEG-9 polydimethylsiloxyethyl dimethicone (Note 2) 3
(5) Decamethylcyclopentasiloxane 20
(6) Isotridecyl isononanoate 5
(7) 2-Ethylhexyl paramethoxycinnamate 8
(8) Diethylaminohydroxybenzoyltetramethylbutylphenol 3.5
(9) Dimethylstearyl ammonium hectorite 1.2
(10) (Dimethicone / Vinyl Dimethicone) Cross Polymer 2.5
(11) Tocopherol 0.001
(12) Remaining amount of purified water
(13) Ethanol 6.5
(14) Sodium chloride 0.3
(15) Fuso extract (* 5) 0.05
(16) Magnesium ascorbyl phosphate 3
(* 5) Prepared according to Production Example 4.
(Note 2) KF-6028 (manufactured by Shin-Etsu Chemical Co., Ltd.)

[Ointment]
(Production method)
A. Ingredients (1) to (4) are heated and mixed and kept at 75 ° C.
B. Ingredients (5)-(9) are heated and mixed and kept at 75 ° C.
C. B was gradually added to A to obtain an ointment.
(Component) (mass%)
(1) Stearic acid 18.0
(2) Cetanol 4.0
(3) dl-α-tocopherol acetate (Note 3) 0.2
(4) Methyl paraoxybenzoate 0.1
(5) Triethanolamine 2.0
(6) Glycerin 5.0
(7) Dipotassium glycyrrhizinate (Note 4) 0.5
(8) Fuso extract (* 6) 0.1
(9) Purified water remaining
(* 6) Prepared according to Production Example 5.
(Note 3) Made by Eisai
(Note 4) Wako Pure Chemical Industries, Ltd.

[Lotion]
(Production method)
A. Components (4) to (7) are mixed and dissolved.
B. Ingredients (1) to (3) are mixed and dissolved.
C. A and B were mixed and homogenized to obtain a lotion preparation.
(Component) (mass%)
(1) Glycerin 5.0
(2) 1,3-butylene glycol 6.5
(3) Purified water remaining
(4) Polyoxyethylene (20E.O.) sorbitan monolaurate ester 1.2
(5) Ethanol 8.0
(6) Fuso extract (* 7) 0.1
(7) Methyl paraoxybenzoate 0.2
(* 7) Prepared according to Production Example 3.

[Hair nourishing agent]
(Production method)
A. Mix and dissolve components (1) to (5).
B. Mix and dissolve components (6) to (10).
C. A and B were mixed and uniformed to obtain a hair nourishing agent.
(Component) (mass%)
(1) Ethanol 50.0
(2) Polyoxyethylene hydrogenated castor oil (80E.O) 0.5
(3) Menthol 0.05
(4) Camphor 0.01
(5) Phenoxyethanol 0.05
(6) Remaining amount of purified water
(7) Fuso extract (* 8) 1
(8) Panax ginseng extract (Note 5) 0.5
(9) Pantothenyl alcohol (Note 6) 0.1
(10) Glycerin 5.0
(* 8) Prepared by Production Example 4
(Note 5) Made by Ichimaru Falcos
(Note 6) Made by Kanto Chemical

  ADVANTAGE OF THE INVENTION According to this invention, the antioxidant (especially in-vivo antioxidant) derived from a natural product, the intracellular oxidative stress inhibitor, the skin external preparation containing them, the cosmetics for anti-aging, and the hair restorer are provided. The

Claims (10)

An antioxidant comprising an extract of a plant belonging to the genus Fusarium as an active ingredient. An in-vivo antioxidant comprising an extract of a plant belonging to the genus Fuyu as an active ingredient. An intracellular oxidative stress inhibitor comprising an extract of a plant belonging to the genus Fusarium as an active ingredient. The agent according to any one of claims 1 to 3, wherein the plant belonging to the genus Fusarium is Fuyu (Liquidambar formosana). The agent according to any one of claims 1 to 4, wherein the extract is an extract of a stem and / or leaf of a plant belonging to the genus Fusarium. The agent according to any one of claims 1 to 5, wherein the extract is obtained using water, a hydrophilic organic solvent, or a mixture thereof as a solvent. The agent according to any one of claims 1 to 6, further comprising ascorbic acid or a derivative thereof, and / or tocopherol or a derivative thereof. The agent of any one of Claims 1-7 for adding to the skin external preparation or cosmetics for anti-aging. The agent according to any one of claims 1 to 8, which is added to a skin external preparation or cosmetic used for hair styling, hair nourishing or hair tonic. A cosmetic method comprising a step of applying an extract of a plant of the genus Fucus to the skin or scalp to suppress oxidative stress in the stratum corneum, epidermis or dermis.

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