JP2011195539A - Elastase activity inhibitor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a novel plant extract derived elastase activity inhibitor having good safety.SOLUTION: The elastase activity inhibitor includes an extract of leaves of Alpinia speciosa (Wendl.) K. Shum in the genus Alpinia of the family Zingiberaceae as an active ingredient. A cosmetic or a skin external preparation including the elastase activity inhibitor inhibits the activity of elastase by applying to the skin and does not cause excessive decomposition of elastin to maintain the flexibility and resilience of the skin and senile signs such as sagging/wrinkles are improved. This cosmetic or skin external preparation has an excellent effect as an anti-aging cosmetic and a wrinkle/sagging improving cosmetic.

Description

  The present invention relates to a novel elastase activity inhibitor.

  Elastin fiber is a type of connective tissue fiber in the extracellular matrix that is distributed in organs and tissues throughout the body, including blood vessels, ligaments, lungs, and skin in the body of mammals and fish such as humans. It is known that the α-helix structure is formed by cross-linking and plays an important role in maintaining the flexibility of the tissue. On the other hand, the skin consists of the epidermis, dermis, and subcutaneous tissue, the epidermis protects the body from external dryness and foreign matter, the subcutaneous tissue softens external impacts by subcutaneous fat, etc., and the dermis is fibroblasts and the outside of these cells. It is constituted by the dermal extracellular matrix that supports the skin structure and plays an important role in maintaining the structure of the skin. In the skin, elastin fibers run parallel to the epidermis surface at the bottom of the papillary layer, the middle fibers of the papillary layer (elaunin fiber), and then the thin terminal fibers come out and go vertically to the basement membrane of the epidermis It is said that there is a thing (oxytalan fiber).

  The phenomenon called skin aging such as skin wrinkle formation, dullness, loss of texture, and loss of elasticity is attributed to a decrease in the production ability of elastin fibers and the like due to a decrease in the proliferative ability of fibroblasts due to aging. However, external irritation such as exposure of the skin to ultraviolet rays, significant drying of the air, excessive skin washing, etc., causes degradation of the elastin fibers, resulting in wrinkle formation, dullness, loss of texture, loss of elasticity, etc. It is said that symptoms such as rough skin and aging of the skin appear.

  It is considered that elastin fibers are degraded by elastase. Elastase is an enzyme belonging to the serine protease family. For example, pancreatic elastase is a marker for various pancreatic diseases because of increased blood levels due to pancreatic lesions. Elastase derived from leukocytes is an early stage that cannot be confirmed with the naked eye. It is known that the blood concentration also rises in the microinflammation. In the skin, for example, it is speculated that there is accumulation of micro-inflammation when irradiated with ultraviolet rays A, and it has been confirmed that elastase activity increases (see, for example, Patent Document 1).

  Therefore, it is said that suppressing the action of elastase in the dermis and preventing the degradation of elastin fibers is effective in preventing aging of skin such as wrinkles and tarmi. For example, Brucella rancifolia extract (see, for example, Patent Document 2) ), Sweet pea extract (for example, refer to Patent Document 3), Iceland moss extract (for example, refer to Patent Document 4), mannentake extract (for example, refer to Patent Document 5), plant / fungal extract, etc. An elastase activity inhibitor contained as an active ingredient has been proposed.

  Further, for example, glucosamine, glucosamine derivatives or salts thereof (for example, see Patent Document 6), phosphonic acid derivatives, mercaptopropionamide derivatives (see, for example, Patent Document 7), corosolic acid (for example, see Patent Document 8). ) And 1,6-heptadiene-3,5-dione derivatives (see, for example, Patent Document 9) have been proposed as elastase inhibitors.

  On the other hand, elastase activity in the skin tissue corresponds to the hair cycle, and it has been reported that an increase in the elastase activity in the skin tissue is essential for hair follicle formation and growth (see, for example, Patent Document 10). A hair growth inhibitor containing an activity inhibitor as an active ingredient has been proposed (see, for example, Patent Document 11).

  Moon peach (Alpinia speciosa (Wendl.) K. Schum.) Is a plant belonging to the genus Alpinia, also known as sannin, and has an antioxidant, antiseptic, and antiseptic effect on the essential oil components obtained from the leaves. It is known to have odor and insect repellent effects, and has been used for a long time in Okinawa and other countries, and many reports have been made on the analysis of essential oil components of moon peach and the use of essential oils. As for the use of the moon peach extract in the cosmetics field, a bathing agent containing, as an active ingredient, a strip obtained by squeezing fresh leaves of Tyling ghetto, which is a kind of moon peach, and pulverized and dried, for example, ghetto A medicinal cosmetic composition (for example, see Patent Document 13) effective for improving acne and rough skin, comprising a extract or compressed liquid from leaves or stems, and moon peach extract (mainly essential oil) as active ingredients A hair restorer or a hair growth agent (for example, see Patent Document 14), an antioxidant containing a moon peach stem extract (for example, see Patent Document 15), etc. are disclosed, and various effects of the moon peach extract are disclosed. Is attracting attention. However, nothing is known about its application as an elastase activity inhibitor in the effectiveness of extracts from moon peach leaves.

Japanese Patent No. 3749769 JP 2002-255734 A JP 2006-282617 A JP 2007-302607 A JP-A-2005-23021 JP 2004-83432 A JP 2006-104209 A JP 2006-265232 A JP 2006-347897 A JP-A-11-199450 JP 2005-343887 A JP 11-349471 A JP 2000-191493 A JP 2000-355525 A JP-A-9-615

  Some of the above-mentioned various elastase activity inhibitors have not been confirmed for safety, stability, etc., and the elastase inhibitory activity effect may not be sufficiently confirmed. Therefore, the subject of this invention is providing the elastase activity inhibitor which uses the compound with the high effect which activates elastase activity as an active ingredient.

  In order to solve the above problems, the present inventors have intensively studied various plant extracts, and as a result, found that the extract from the leaves of moon peach exhibits elastase activity inhibitory effect, and completed the present invention. did.

  According to the present invention, a novel elastase activity inhibitor with good safety can be provided.

The figure which shows the elastase activity inhibitory effect evaluation test and its result.

  Hereinafter, the present invention will be described in detail. In the present specification, “to” means a range including numerical values before and after.

  The moon peach (Alpinia speciosa (Wendl.) K. Schum.) Used in the present invention is a plant belonging to the genus Alpinia, also known as Sannin. The present invention uses an extract from a moon peach leaf (hereinafter abbreviated as a moon peach leaf extract).

  The moon peach leaf extract used in the present invention can be prepared by a general method. Although it does not specifically limit as an extraction solvent, For example, Water; Lower monohydric alcohols, such as methyl alcohol and ethyl alcohol; Liquid polyhydric alcohols, such as glycerol, propylene glycol, and 1, 3- butylene glycol; Ketones, such as acetone; Ethyl One or more of ethers such as ether; esters such as ethyl acetate; and the like can be used. The extraction can be carried out by immersing the moon peach leaves in a solvent at room temperature or under heating for a predetermined time. Moreover, pre-processing, such as drying, shredding, pressing or fermentation, can also be performed before extraction.

  The moon peach leaf extract can be used directly as an elastase activity inhibitor after preparation. Further, if desired, it can be used as an elastase activator after being left to mature for an appropriate period. If necessary, it can be used after being subjected to a purification treatment such as deodorization and decolorization by filtration or ion exchange resin within a range that does not affect the effect of the present invention. Further, a fraction having high activity can be taken out and used by using a separation means such as liquid chromatography.

  The moon peach leaf extract may be in any form such as liquid, paste or gel. That is, the liquid extract containing the extraction solvent may be used as it is or after being concentrated, or may be used after the extract is dried and solidified by drying under reduced pressure or freeze drying. It can also be dried by spray drying or the like and used as a powder. Furthermore, these solid or powder extracts may be appropriately redissolved in a solvent and used as an extract.

  The elastase activity inhibitor of the present invention can be used for various applications. Since the elastase activity inhibitor of the present invention has high safety and excellent stability at the time of blending, it can be used as an active ingredient for blending into foods (including beverages) and cosmetics or skin external preparations. Is preferred.

The cosmetic or external preparation for skin containing the elastase activity inhibitor of the present invention inhibits the activity of elastase when applied to the skin. As a result, excessive degradation of elastin does not occur, skin flexibility and elasticity are maintained, and senile phenomena such as sagging and wrinkles are improved. That is, it has excellent effects as an anti-aging cosmetic and a wrinkle / sag improving cosmetic.
Further, since elastase may be involved in immune cell migration in an inflammatory reaction, the cosmetic or skin external preparation containing the elastase activity inhibitor of the present invention is a cosmetic or skin external preparation for inflammatory activity. It is also useful.
Furthermore, since elastase may be involved in hair follicle formation, the cosmetic or skin external preparation containing the elastase activity inhibitor of the present invention is a cosmetic or skin external preparation that controls hair growth and hair growth. It is also useful.

  The blending amount of the elastase activity inhibitor of the present invention in the cosmetic or external preparation for skin is not particularly limited, but in general, it is converted into a dry solid content with respect to the total composition from the viewpoint of elastase activity. The content is preferably 0.0001 to 1% by mass (hereinafter simply expressed as%), more preferably 0.001 to 0.1%. Within this range, the elastase activity inhibitor of the present invention can be stably blended, and a high anti-aging effect and sagging / wrinkle improving effect can be exhibited.

  In the cosmetic or external preparation for skin, other active ingredients such as a whitening effect, an anti-aging effect, or an effect of suppressing wrinkle formation due to exposure to ultraviolet rays are within the range not impairing the effects of the present invention. Ingredients may be blended. More specifically, examples include, but are not limited to, UV protection agents, antibacterial agents, whitening agents, anti-inflammatory agents, cell activators, active oxygen scavengers, and moisturizers.

  Examples of UV protective agents include benzophenone compounds, PABA compounds, cinnamic acid compounds, salicylic acid compounds, 4-tert-butyl-4′-methoxydibenzoylmethane, and p-methoxycinnamic acid-2-ethylhexyl. Para-methoxycinnamic acid-2-ethylhexyl, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sodium sulfate, 4-t-butyl-4'-methoxydibenzoylmethane, 2- Mention may be made of phenyl-benzimidazole-5-sulfate, titanium oxide, zinc oxide.

  Examples of the antibacterial agent include benzoic acid, sodium benzoate, p-hydroxybenzoate ester, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, and the like.

  The whitening agent has the effect of preventing the occurrence of darkening of the skin caused by sunburn or the like, stains caused by pigmentation, buckwheat, etc., such as arbutin, ellagic acid, linoleic acid, vitamin C and derivatives, vitamin E and Its derivatives, glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, tranexamic acid, placenta extract, chamomile extract, licorice extract, age extract, sea urchin extract, seaweed extract, cucumber extract, coconut extract, Gokahi extract, rice bran extract, wheat germ extract, saicin extract, hawthorn extract, sunpens extract, shirayuri extract, peonies extract, sempukuka extract, soybean extract, tea extract, molasses extract, beechlen Extract, Grape extract, Hop extract, Mikaika extract, Mokka extract, Yu Noshita extract, and the like.

  Anti-inflammatory agents have the effect of suppressing inflammation such as hot flashes and erythema on the skin after sunburn. For example, sulfur and its derivatives, glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, Altea extract, Ashitaba Extract, Arnica extract, Ginseng extract, Nettle extract, Oat extract, Hypericum extract, Chamomile extract, Snapdragon extract, Watercress extract, Comfrey extract, Salvia extract, Shikon extract, Perilla extract Product, birch extract, gentian extract and the like.

  The cell activator is used for the purpose of improving rough skin, such as caffeine, chicken crown extract, shell extract, royal jelly, silk protein and its degradation product or derivatives thereof, lactoferrin or its degradation product, chondroitin sulfate, Mucopolysaccharides such as hyaluronic acid or their salts, collagen, yeast extract, lactic acid bacterium extract, bifidobacteria extract, fermentation metabolic extract, ginkgo biloba extract, barley extract, sembly extract, beetle extract, carrot extract Products, rosemary extract, glycolic acid, citric acid, lactic acid, malic acid, tartaric acid, succinic acid and the like.

  The active oxygen scavenger has an action such as a lipid peroxide production inhibitor, such as superoxide dismutase, mannitol, quercetin, catechin and derivatives thereof, rutin and derivatives thereof, button pi extract, yashajitsu extract, melissa Extracts, Rahan fruit extract, retinol and its derivatives, vitamin A such as carotenoid, thiamine and its derivatives, riboflavin and its derivatives, pyridoxine and its derivatives, vitamin B such as nicotinic acid and its derivatives, tocopherol and its derivatives, etc. Vitamin E, dibutylhydroxytoluene and butylhydroxyanisole.

  Examples of the humectant include proteins such as elastin and keratin or derivatives thereof, hydrolysis and salts thereof, amino acids such as glycine, serine, asoaraginic acid, glutamic acid, arginine, and theanine and derivatives thereof, sorbitol, erythritol, trehalose. , Inositol, glucose, sucrose and derivatives thereof, dextrin and derivatives thereof, saccharides such as honey, D-panthenol and derivatives thereof, urea, phospholipid, ceramide, auren extract, shobu extract, diox extract, sensyu extract , Mushroom extract, gypsophila extract, dokudami extract, hamamelis extract, bodaige extract, maronier extract, quince extract and the like.

  In addition, the cosmetic or skin external preparation may be used in the manufacture of cosmetics, quasi drugs, skin external preparations, and the like, such as water (purified water, Hot spring water, deep water, etc.), oil agent, surfactant, metal soap, gelling agent, powder, alcohol, water-soluble polymer, film-forming agent, resin, inclusion compound, antibacterial agent, fragrance, deodorant , Salts, pH adjusters, fresheners, extracts derived from plants, animals and microorganisms, active oxygen scavengers, blood circulation promoters, astringents, antiseborrheic agents, moisturizers, chelating agents, keratolytic agents, enzymes, hormones Other vitamins can be added as needed.

  Moreover, what mix | blends the elastase activity inhibitor of this invention with foodstuffs (a drink is included) is also preferable. Although it does not restrict | limit especially as said foodstuff, Specifically, a salmon, chewing gum, a drink etc. are mentioned, for example.

Hereinafter, the present invention will be described more specifically with reference to examples. However, the scope of the present invention is not limited to the following examples.
[Production Example 1: Preparation of moon peach leaf extract]
100 g of moon peach leaf was pulverized, placed in 1 L of 80 mass% ethanol aqueous solution, and extracted by heating under reflux. The insoluble material was filtered off, and the filtrate was concentrated under reduced pressure to obtain a moon peach leaf extract. The extract here was dried and collected as a solid.
[Example 1: Elastase activity inhibition test]
As test solutions, 0.001%, 0.01%, and 0.1% solutions were prepared as the solid content concentration of the moon peach extract. The sample used here was a mixed solution with a volume ratio of ethanol: water = 1: 1.
The elastase activity inhibition test was performed using elastase enzyme solution derived from swine and N-Succinyl-Ala-Ala-Ala-p-nitroside as a substrate, specifically, as follows.
The enzyme solution is 0.05
The concentration was adjusted to units / mL.
The substrate solution was prepared by dissolving the substrate in dimethyl sulfoxide (DMSO) to obtain a 0.1 mol / L concentration solution, and then diluting with 0.05 mol / L Tris-HCl (pH 8.8) buffer solution to obtain 1 mmol. / L concentration solution was prepared.
After mixing 100 μL of the substrate solution and 50 μL of the 1% sample solution, 50 μL of the enzyme solution was added and reacted at 37 ° C. for 30 minutes. Thereafter, the absorbance at a wavelength of 405 nm was measured with a spectrophotometer, the amount of produced nitroaniline was determined, and the elastase activity inhibition rate was calculated. The elastase activity inhibition rate was calculated by the following formula.
Elastase activity inhibition rate = {1- (AB) / (CD)} × 100
A: Absorbance at a wavelength of 405 nm of the mixed solution when the enzyme solution is added after mixing the substrate solution and the sample solution B: Absorbance at a wavelength of 405 nm of the mixed solution when the substrate solution and the sample solution are mixed C: Sample Instead of the solution, after the solvent of the solution is mixed with the substrate solution in the above ratio, the absorbance at a wavelength of 405 nm of the mixture solution when the enzyme solution is added. D: The sample solution and the enzyme solution are not added. Absorbance at 405 nm

Moreover, in order to verify the elastase activator effect of the moon peach leaf extract of the present invention, a complete protease inhibitor cocktail manufactured by Roche was used as a comparison target. The protease inhibitor cocktail used in the present invention always shows a certain ability of protease activity, is a reagent that activates various protease activities, and has been confirmed to activate elastase activity. The results are shown in FIG. 1 together with the results of the moon peach leaf extract.
From the results shown in the graph of FIG. 1, it was found that the elastase activity inhibitor of the present invention has a remarkable elastase activity inhibitory action even at low concentrations with respect to the complete protease inhibitor cocktail. Therefore, by using the elastase activity inhibitor of the present invention, a higher elastase activity inhibitory action can be obtained. In addition, even when the blending amount is reduced from the conventional level, the same or higher effect can be obtained. Therefore, the range of selection of the blending composition is widened, and it is advantageous in terms of formulation to use the elastase activity inhibitor of the present invention.

The following are examples of the elastase activity inhibitor of the present invention and cosmetics or skin external preparations containing the elastase activity inhibitor.
[Production Example 2: Preparation of moon peach leaf extract]
100 g of moon peach leaves were pulverized and placed in 1 L of 30% 1,3-butylene glycol aqueous solution for extraction. After filtering the insoluble matter, the filtrate was concentrated under reduced pressure, and 1,3-butylene glycol and purified water were added to prepare a 1,3-butylene glycol concentration of 30%. The solid content concentration of this extract was 2.0%.

[Example 2: Face wash]
(Manufacturing method)
A. The following components (1) to (7) are dissolved by heating.
B. The following components (8) to (11) are dissolved by heating.
C. Add B to A and mix.
D. After cooling C, the following components (12) to (14) were added and mixed to obtain a face wash.

(Ingredient) (%)
(1) Lauric acid 5.0
(2) Myristic acid 18.5
(3) Stearic acid 6.0
(4) Glycerin 12.0
(5) Polyethylene glycol 1500 5.0
(6) Potassium hydroxide 6.5
(7) Purified water remaining amount (8) Palm oil fatty acid diethanolamide 5.0
(9) Palm oil fatty acid methyl taurine sodium 1.8
(10) Polyoxyethylene (7.5E.O.) lauryl ether
2.0
(11) Ethylene glycol distearate 1.0
(12) Hydroxypropyl methylcellulose 1% aqueous solution 5.0
(13) Moon peach leaf extract of Production Example 1 0.01
(14) Perfume appropriate amount

[Example 3: lotion]
(Manufacturing method)
A. The following components (1) to (6) are mixed and dissolved.
B. The following components (7) to (12) are mixed and dissolved.
C. B was added to A and mixed to obtain a skin lotion.

(Ingredient) (%)
(1) Citric acid 0.05
(2) Sodium citrate 0.2
(3) Sodium pyrrolidonecarboxylate (50%) solution 0.5
(4) Dipotassium glycyrrhizinate 0.1
(5) Glycerin 3.0
(6) 1,3-butylene glycol 8.0
(7) Purified water remaining amount (8) Ethanol 10.0
(9) Moon peach leaf extract of Production Example 1 0.1
(10) Perfume Appropriate amount (11) Preservative Appropriate amount (12) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5

[Example 4: Latex (oil-in-water type)]
(Manufacturing method)
A. The following components (1) to (13) are dissolved by heating and maintained at 70 ° C.
B. The following components (14) to (19) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and the following component (20) is further added and mixed.
D. C is cooled, and the following component (21) is added and mixed to obtain an emulsion.

(Ingredient) (%)
(1) Stearic acid 1.0
(2) Cetanol 0.5
(3) Lipophilic glyceryl monostearate 0.5
(4) Liquid paraffin 2.0
(5) Squalane 3.0
(6) Jojoba oil 3.0
(7) Cetyl palmitate 0.2
(8) Retinol palmitate 0.2
(9) Tocopherol acetate 0.05
(10) Preservative appropriate amount (11) Sorbitan monostearate 0.3
(12) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(13) Dibutylhydroxytoluene 0.1
(14) Triethanolamine 0.5
(15) 1,3-butylene glycol 15.0
(16) Glycerol 3.0
(17) Polyethylene glycol 6000 0.5
(18) Moon peach leaf extract of Production Example 2 1.5
(19) Purified water remaining amount (20) 1% solution of carboxyvinyl polymer 8.0
(21) Perfume appropriate amount

[Example 5: Cream (oil-in-water type)]
(Manufacturing method)
A. The following components (1) to (14) are dissolved by heating and maintained at 70 ° C.
B. The following components (15) to (20) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and the following component (21) is further added and mixed.
D. C was cooled, the following component (22) was added and mixed to obtain a cream (oil-in-water type).

(Ingredient) (%)
(1) Stearic acid 2.5
(2) Cetanol 2.5
(3) Lipophilic glyceryl monostearate 2.0
(4) Vaseline 2.0
(5) Dipentaerythritol fatty acid ester 2.0
(6) Isotridecyl myristate 5.0
(7) Liquid paraffin 8.0
(8) Squalane 5.0
(9) Beeswax 1.0
(10) Cetyl palmitate 2.0
(11) Sorbitan sesquioleate 0.5
(12) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.5
(13) Coenzyme Q10 0.1
(14) Preservative appropriate amount (15) Triethanolamine 1.2
(16) 1,3-butylene glycol 8.0
(17) Glycerin 2.0
(18) Polyethylene glycol 20000 0.5
(19) Moon peach leaf extract of Production Example 2 2.0
(20) Purified water remaining amount (21) Carboxyvinyl polymer 1% aqueous solution 10.0
(22) Perfume appropriate amount

[Example 6: Water-in-oil type sunscreen cream]
(Manufacturing method)
A. The following components (1) to (8) were heated and mixed at 70 ° C.
B. The following components (9) to (12) and (14) to (15) were heated and mixed at 50 ° C.
C. B was added to A to emulsify, and after cooling, (13) was added to obtain a water-in-oil sunscreen cream.

(Ingredient) (%)
(1) Polyoxyalkylene-modified organopolysiloxane (Note 4) 2.0
(2) Octyl palmitate 15.0
(3) Decamethylcyclopentasiloxane 20.0
(4) Glyceryl tribehenate 1.0
(5) Fine zinc oxide 12.0
(6) Fine particle titanium oxide 3.0
(7) 2-Ethylhexyl paramethoxycinnamate (Note 5) 7.0
(8) 4-tertbutyl-4′-methoxydibenzoylmethane
(Note 6) 1.0
(9) Dipropylene glycol 5.0
(10) Ethanol 5.0
(11) Polyethylene powder 3.0
(12) Preservative Appropriate amount (13) Fragrance Appropriate amount (14) Moon peach leaf extract of Production Example 1 0.003
(15) Remaining amount of purified water (Note 4) KF-6017 (manufactured by Shin-Etsu Chemical Co., Ltd.)
(Note 5) Yubinar MC80 (BASF)
(Note 6) PARSOL 1789 (manufactured by LC UNITED)

[Example 7: Pack cosmetic]
(Manufacturing method)
A. The following components (1) to (5) and (15) are heated and mixed at 70 ° C. and cooled to room temperature.
B. The following components (6) to (14) were added to A and mixed to obtain a pack cosmetic.

(Ingredient) (%)
(1) Polyvinyl alcohol 15.0
(2) Glycerin 10.0
(3) Polyoxyethylene (10) methyl glycol 3.0
(4) Glyceryl trioctanoate 5.0
(5) Sodium polyoxyethylene alkyl ether phosphate 1.0
(6) Ethanol 20.0
(7) Kaolin 2.0
(8) Titanium oxide 2.0
(9) Dipotassium glycyrrhizinate 0.1
(10) Lactic acid (50% aqueous solution) 0.5
(11) Sodium lactate (50% aqueous solution) 0.5
(12) Preservative Appropriate amount (13) Fragrance Appropriate amount (14) Moon peach leaf extract of Production Example 1 0.05
(15) Purified water remaining

[Embodiment 8: Liquid foundation (oil-in-water type)]
(Manufacturing method)
A. The following components (1) to (7) are heated and mixed at 70 ° C., and the following components (13) to (18) are added to the mixture and mixed to maintain at 70 ° C.
B. The following components (8) to (12) are heated and mixed at 70 ° C.
C. A was added to B to emulsify, and after cooling, the following components (19) to (20) were added to obtain a liquid foundation (oil-in-water type).

(Ingredient) (%)
(1) Dipentaerythritol fatty acid ester 2.0
(2) Liquid paraffin 5.0
(3) Stearic acid 2.0
(4) Cetanol 1.0
(5) Glyceryl monostearate 1.0
(6) 2-Methylhexyl paramethoxycinnamate 8.0
(7) Preservative appropriate amount (8) Glycerin 5.0
(9) Triethanolamine 1.0
(10) Carboxymethylcellulose 0.2
(11) Bentonite 0.5
(12) Purified water remaining amount (13) Titanium oxide 6.0
(14) Fine particle titanium oxide 2.0
(15) Fine zinc oxide 4.0
(16) Mica 2.0
(17) Talc 4.0
(18) Color pigment appropriate amount (19) Moon peach leaf extract of Production Example 1 0.01
(20) Perfume appropriate amount

Each of the various cosmetics prepared above is excellent in stability over time, and when applied to the skin, improves the wrinkles and sagging of the skin, and makes the skin beautiful and firm, with excellent anti-aging effects. there were.

Claims (1)

Ginga (Zingiberaceae) family Alpinia speciosa (Alpinia speciosa)
(Wendl.) An elastase activity inhibitor comprising a moon peach leaf extract extracted from the leaves of K. Schum.