JP3846680B2 - Cosmetics - Google Patents

Description

【００４４】
表１の結果から明らかな通り、出穂前のイネの葉及びイネの幼苗の葉から得られる抽出物は、いずれも過酸化脂質の生成を顕著に抑制し、強い抗酸化作用を示す。
なお、製造例１と同様の出穂前のイネの葉を加熱酵素失活処理を施すことなくそのまま天日に３０日間曝露して得た乾燥葉、及び出穂１０日後に採取したイネ（コシヒカリ）の生葉を用い、それぞれ製造１及び２と同様の処理を行って調製したイネ抽出物溶液について、過酸化脂質抑制作用を調べたところ、４日目の過酸化脂質生成量は、いずれも製造例２或いは７のイネ抽出物の5〜１０倍に達し、イネ採取後の長期間の保管或いは出穂によりイネの抗酸化力が低下する傾向が認められた。
【００４５】
試験例２ イネの線維芽細胞活性化作用
［試料］
（１）製造例２のイネ抽出物溶液
（２）製造例７のイネ抽出物溶液
【００４６】
［試験方法］
ヒト真皮由来線維芽細胞NB1RGB(000824）を、０．５％FCS含有最少必須培地を入れた９６穴マイクロプレートに１×１０^４ 個／穴播種し、３７℃で１日間プレ培養した後、培地に試料溶液を２．５％又は５．０％の濃度となるように添加し、３７℃でさらに６日間培養した。次に、培地を除去し界面活性剤(TRITON X-100）を添加した細胞処理液に、０．２％のＭＴＴを添加して３７℃に保持した後、マイクロプレートリーダー(Model 1450、バイオラッド社製）を用い、波長370−630nmでＭＴＴ値を測定した。
なお、比較のため、イネ抽出物溶液の代わりにグルコースを５０ｍＭ添加した場合及び試料無添加の場合（ブランク）についても、同様の試験を行った。
【００４７】
［結果］
結果を図１に示す。
図１に示す通り、本発明のイネ抽出物は、線維芽細胞に対するＭＴＴ活性を亢進し、該細胞を活性化する作用を有する。
【００４８】
試験例３ 皮膚刺激性評価試験
眼粘膜刺激性試験の代替法である培養ウサギ角膜細胞（ＳＩＲＣ）を用いたニュートラルレッド取り込み試験により、イネ抽出物の皮膚刺激性を評価した。なお、比較のため公知の抗酸化剤であるα-トコフェロールについても同様の評価を行った。
【００４９】
［試料］
（１）製造例８の抽出物粉末
（２）α-トコフェロール（比較試料）
【００５０】
［試験方法］
ＳＩＲＣを９６穴マイクロプレートに５０００個／穴播種し、５％仔牛血清含有イーグル最少必須培地（ＭＥＭ）で３日間培養した後、これに試料を０．２％の濃度となるように添加し、さらに２日間培養した。次に、ＳＩＲＣを０．０５％ニュートラルレッド（ＮＲ）含有イーグルＭＥＭ培地で３時間培養し、ここに得られたＳＲＩＣについてＮＲの取り込み率を求め、その値から各試料の皮膚刺激性を評価した。
【００５１】
［結果］
結果を表２に示す。
【表２】

【００５２】
表２に示す通り、本発明のイネ抽出物は、既存の抗酸化剤に比べて皮膚に対する刺激性が少なく、安全性にすぐれた化粧料を提供することが可能である。
【００５３】
試験例４ モニターテスト
実施例１、９と比較例１、２の各クリームについて、モニターテストにより皮膚に対する効果を調べた。
［試験方法］
無作為に抽出した年齢１８〜５０歳の女性４０名を被験者として１０名ずつ４つのグループに分け、各グループに、実施例と比較例のクリームの４種の組み合わせのいずれかを割り振り、それぞれ左右の頬部に、実施例又は比較例のクリームを１日２回（朝、晩）、1ヵ月間塗布してもらった後、肌のくすみ、シミに対する改善効果、小ジワに対する改善効果及び肌のはり、艶に対する改善効果を、以下の評価基準に基づいて評価した。
【００５４】
［評価基準］
（肌のくすみ、シミに対する改善効果）
Ａ：非常に改善された
Ｂ：かなり改善された
Ｃ：僅かに改善された
Ｄ：変わらない
Ｅ：かえって目立つようになった
（小ジワに対する改善効果）
Ａ：殆ど目立たなくなった
Ｂ：かなり目立たなくなった
Ｃ：わずかに目立たなくなった
Ｄ：変わらない
Ｅ：かえって増えた
（肌のはり、艶に対する改善効果）
Ａ：明らかに改善された
Ｂ：かなり改善された
Ｃ：僅かに改善された
Ｄ：変わらない
Ｅ：かえって悪くなった
【００５５】
［結果］
結果を表３に示す。
【００５６】
【表３】

【００５７】
表３に示す通り、本発明のイネ抽出物を配合したクリームは、従来の皮膚老化防止化粧料に勝るすぐれた皮膚老化防止作効果、肌荒れ改善効果を示す。
【００５８】
【発明の効果】
イネの葉の抽出物からなる本発明の配合剤は、イネの葉の抽出物が抗酸化作用と線維芽細胞活性化作用を併せ持ち、皮膚適用時それら作用が相乗的に働く結果、皮膚の老化防止並びに肌荒れ改善に於いて極めてすぐれた効果を発揮する。このため、該配合剤を含有してなる本発明の化粧料は、従来の老化防止化粧料に比してより改善された皮膚老化防止効果、肌荒れ改善効果を示す。又、本発明の配合剤は天然物由来のものであるため皮膚に対する刺激が少なく、従ってこれを配合した化粧料は安全性にすぐれている。
【図面の簡単な説明】
【図１】 図１は、試験例２の各試料のＭＴＴ値を示すグラフである（縦軸：ＭＴＴ値）。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a cosmetic compounding agent having an excellent effect in preventing wrinkles and spots due to aging of skin, and the prevention or improvement of symptoms such as rough skin, and also has a high skin safety and a cosmetic containing the compounding agent.
[0002]
[Prior art]
Skin aging is caused by internal factors such as inactivation of cell growth / differentiation associated with aging, decreased secretion of hormones, and cell / tissue damage or inflammation caused by active oxygen induced by exposure to sunlight (ultraviolet rays). This is a phenomenon that is caused by complex intertwining of external factors. Skin morphological and physiological changes appear as symptoms such as wrinkles, sagging, spots, and rough skin.
In order to prevent this skin aging and to keep the skin healthy and youthful, the use of various active ingredients has been proposed and cosmetics containing these ingredients have been put on the market. For example, antioxidants such as vitamin C and vitamin E, anti-inflammatory agents such as glycyrrhetinic acid, various UV absorbers, α-hydroxycarboxylic acid, placental extract, and cell activation such as γ-amino-β-hydroxybutyric acid Ingredients, skin matrix components such as collagen and elastin, and moisturizers such as hyaluronic acid and urea.
However, since these ingredients generally only serve to improve or eliminate one of the above-mentioned skin aging factors, depending on conventional cosmetics containing these ingredients, skin aging that is truly satisfactory At present, the prevention effect cannot be obtained. Some types of ingredients still require improvement in terms of skin safety, such as increasing the blending amount to increase effectiveness and causing skin irritation.
[0003]
[Problems to be solved by the invention]
The present invention has been made in view of the above-mentioned problems found in conventional cosmetics, and the object of the present invention is to be complex and multifaceted with respect to various factors such as skin aging and rough skin. It is an object of the present invention to provide a new cosmetic compounding agent capable of maintaining the skin in a healthy and youthful state by acting and a cosmetic containing the compounding agent.
Another object of the present invention is to provide a cosmetic composition comprising the above-mentioned cosmetic composition containing an ingredient derived from a natural product as an active ingredient and excellent in safety to the skin, and a cosmetic comprising the composition.
Still other objects of the present invention will become apparent from the following description.
[0004]
[Means for Solving the Problems]
That is, the present invention extracts rice (Oryza sativa) leaves with a solvent selected from water, lower alcohols, polyhydric alcohols, a mixture of water and lower alcohols, and a mixture of water and polyhydric alcohols. antioxidant and fibroblast activation agent comprising as an active ingredient an extract showing antioxidant and fibroblast activation action obtained, and written that antioxidant and that it has formulated a fibroblast activator It is a characteristic cosmetic.
Extract of leaves of rice of the present invention, as shown in Test Examples later, antioxidant and further combines the fibroblast activating activity, the synergistic effect based on the composite of these effects, skin aging, skin roughness Exhibits excellent effects in prevention or symptom improvement. Needless to say, the rice leaf extract has no skin irritation and is extremely safe for the skin. .
[0005]
Hereinafter, the present invention will be described in detail.
As the rice in the present invention, any of those belonging to Oryza sativa can be used for taxonomics, and specific examples include Koshihikari, Sasanishiki, Nihonbare, Akita Komachi, Kinuhikari, and Hua Echizen. However, of course, it is not necessarily limited to these.
In the present invention, an extract showing antioxidation and fibroblast activation activity obtained by extraction from water, alcohols or a mixture of water and alcohols from these rice leaves is used as an antioxidant and fibroblast. It is used as a cell activator or such an agent is blended in cosmetics.
In this case, as rice leaves, it is most preferable to use rice leaves before heading in terms of effect. Moreover, what germinated and grew again from the rice stock after cutting rice can also be used.
[0006]
The rice leaf extract is prepared, for example, by the method described below.
That is, the collected rice leaves (hereinafter, rice leaves may be simply referred to as rice) are washed with water and then cut into several centimeters to several tens of centimeters as necessary, and then preferably heat-treated. Stops enzyme activity in rice. Thus, modification by enzymes of the active ingredients contained in rice, alteration is suppressed, decrease in activity during storage of the harvested rice, or a result of temporal decrease in activity of rice extract is prevented, high activity it is possible to obtain rice extract having good storage stability.
Therefore, the heat treatment is particularly important in obtaining the highly effective agent and cosmetic of the present invention, and it is important that the heat treatment be performed within as short a day as possible after the rice is collected. It is. The time when it is desirable to perform the heat treatment after rice collection differs depending on the rice varieties, harvest time, etc., but it cannot be said unconditionally, but as an indicator, the color of rice can be mentioned, and in particular the harvested rice It is desirable to perform the treatment while the green color is not completely faded and is still maintained.
The heat treatment is preferably performed by wet heat treatment (steaming), and in the case of steaming, the time is generally in the range of 10 seconds to 5 minutes, preferably 15 seconds to 3 minutes, particularly preferably 20 seconds to 1 minute. If the steaming time exceeds the above, the antioxidant activity of the rice extract or pulverized product tends to decrease, which is not preferable.
[0007]
Preparation of rice extract, as the rice finished heat enzyme-inactivation treatment, or even either immersed in minced to extraction in a solvent, if necessary, more preferably a dry pulverized i ne It is carried out by a method of mixing with an extraction solvent.
As an extraction solvent, water; lower alcohols such as methanol, ethanol, propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, 1,2-pentanediol, glycerin; water and the above-mentioned A solvent selected from a mixed solution of such lower alcohols and a mixed solution of water and the above polyhydric alcohols is used. Among these, it is particularly preferable to use water or a mixture of water and lower alcohols or a mixture of water and polyhydric alcohols.
[0008]
Extraction conditions vary depending on the degree or grain size of rice, the type of extraction solvent, etc., but generally 4 to 100 times, preferably 10 to 20 times the amount of solvent is used. Extraction is preferably performed at -80 ° C for 1 hour to 7 days, particularly at 20-60 ° C for 2 to 48 hours.
The extract solution obtained here is generally clarified by filtration. If necessary, the pH is adjusted to 4-8, and the antioxidant and the fibroblast activator are adjusted to appropriate concentrations as they are or by concentration under reduced pressure. It is used as a. In some cases, it may be used after being powdered according to a conventional method such as spray drying or freeze drying.
[0009]
The amount of the cosmetic in consists in the above rice extract antioxidant and fibroblast activator of the present invention, a cream, a milk, if basic cosmetics such as lotions, generally 0.001 to 0.5 wt% (as a solid content. hereinafter the same), preferably in the range of 0.01 to 0.2 wt%, if blusher, makeup cosmetics such as face powder, generally 0.001 to 0.5 wt%, preferably from 0.01 to 0.1 wt% range, if the hair cosmetic, generally 0.001 to 0.5% by weight, preferably the range of 0.01 to 0.2 wt%, if bath agent, generally 0.01 to 1% by weight, preferably 0.05 to 0.5 wt% it is a range.
[0010]
In addition to antioxidants and fibroblast activators composed of essential rice extract, the cosmetics of the present invention include components usually used in cosmetics such as oily components, surfactants, moisturizers, thickeners. An agent, an antiseptic / bactericidal agent, a powder component, an ultraviolet absorber, an antioxidant, a pigment, a fragrance and the like are appropriately blended as necessary.
[0011]
Here, as the oily component, for example, oils derived from plants such as olive oil, jojoba oil, castor oil, soybean oil, coconut oil, palm oil, cacao oil, and meadow foam oil; derived from animals such as mink oil and turtle oil Fats and oils; waxes such as beeswax, carnauba wax and lanolin; hydrocarbons such as liquid paraffin, petrolatum, paraffin wax and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid and isostearic acid; lauryl alcohol; Examples include higher alcohols such as cetanol and stearyl alcohol; synthetic esters such as isopropyl myristate, butyl oleate, and 2-ethylhexyl glyceride, and synthetic triglycerides.
[0012]
Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N Cationic surfactants such as N, N-dialkylmorphonium salt, polyethylene polyamine fatty acid amide salt; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N- Examples include amphoteric surfactants such as alkylene ammoniocarboxybetaine, N-acylamidopropyl-N ′, and N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine.
[0013]
Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, pyrrolidone carboxylic acid sodium and the like, and sugars, hyaluronic acid and its derivatives, lactic acid, urea, higher Examples include fatty acid octyldodecyl.
[0014]
Examples of the thickener include polysaccharides such as carrageenan, alginic acid, pectin and locust bean gum; gums such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; polyvinyl alcohol, polyvinylpyrrolidone and carboxyvinyl polymer And synthetic polymers such as acrylic acid / methacrylic acid copolymer; hyaluronic acid and derivatives thereof.
[0015]
Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzal chloride Luconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea) and the like.
[0016]
Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, and nylon powder.
[0017]
Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, ethylhexyl paramethoxycinnamate, octyl cinnamate, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone- 5-sulfonate, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate and the like.
[0018]
Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (for example, vitamin E nicotinate and vitamin E linoleate).
In addition, lecithins, silk-related substances and the like can be blended as other components.
[0019]
The cosmetic of the present invention may further contain other active ingredients as long as the effectiveness of the components of the present invention is not impaired. Examples of such compounds include kojic acid and derivatives thereof (for example, kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate or kojic acid ethers), arbutin, ascorbine, etc. Acids and derivatives thereof [for example, ascorbic acid esters such as L-ascorbic acid-2-phosphate and L-ascorbic acid-2-sulfate (salts are sodium salt, magnesium salt, etc.); L-ascorbic acid Ascorbic acid coordination such as 2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-O-α-D-glucopyranosyl-L-ascorbic acid) Sugars, etc.], ellagic acid, resorcinol derivatives (for example, 4 Whitening ingredients such as n-butylresorcinol, 4-isoamylresorcinol), Sakuhachi extract, yukinoshita extract, rice bran extract, 2,5-dihydroxybenzoic acid derivatives (eg 2,5-diacetoxybenzoic acid, etc.); Extract, collagen, nicotinic acid and its derivatives (eg, nicotinamide, benzyl nicotinate, etc.), α-hydroxy acids (eg, lactic acid, citric acid, α-hydroxyoctanoic acid, etc.), diisopropylamine dichloroacetate, γ-amino- Skin aging prevention / roughness improving components such as β-hydroxybutyric acid; herbal medicine extract such as gentian extract.
[0020]
There is no particular limitation on the form of the cosmetic comprising the antioxidant and fibroblast activator comprising the rice extract of the present invention. For example, the basis of cream, emulsion, lotion, essence, facial cleanser, pack, etc. Cosmetics, lipsticks, foundations, liquid foundations, pressed powders, cheeks, white powders and other makeup cosmetics, hair restorers, treatments, hair tonics such as tonics, bath preparations and the like.
[0021]
Next, Production Examples, Examples (punished Horei) and Test Examples will be described the present invention will be more specifically, the present invention is not limited thereto. In the following, all parts are by weight, and all% are by weight.
[0022]
Production Example 1 Preparation of pulverized rice The raw leaves of rice (Koshihikari) collected just before heading (blossoming stage) were shredded immediately after collection, exposed to steam for 30 seconds, heat-treated, and then dried at 30 ° C. After that, the mixture was pulverized using an airflow pulverizer to prepare a pulverized rice product (particle size: about 50 mesh).
[0023]
Production Example 2 Preparation of Rice Extract Solution (1)
950 g of purified water was mixed with 50 g of the pulverized rice product obtained in Production Example 1 and extracted at 40 ° C. for 3 hours, followed by filtration to obtain 720 ml of a yellowish brown transparent rice extract solution (solid content concentration: 0). .76%).
[0024]
Production Example 3 Preparation of Rice Extract Solution (2)
Purified water 900 g was mixed with 100 g of the pulverized rice product obtained in Production Example 1, and extraction was performed at 40 ° C. for 6 hours, followed by filtration to obtain 660 ml of a brown transparent rice extract solution (solid content concentration: 1. 53%).
[0025]
Production Example 4 Preparation of Rice Extract Solution (3)
In Production Example 2, yellow-brown transparent rice extraction was carried out in the same manner as in Production Example 2 except that purified water / 1,3-butylene glycol = 70/30 (volume ratio, the same applies hereinafter) was used instead of purified water. A solid solution (700 ml) was obtained (solid content concentration: 0.66%).
[0026]
Production Example 5 Preparation of Rice Extract Solution (4)
In Production Example 2, a mixed solution of purified water / ethanol = 80/20 was used instead of purified water, and extraction was performed at room temperature for 3 days. Obtained (solid content concentration: 0.70%).
[0027]
Production Example 6 Preparation of Rice Extract Solution (5)
In Production Example 2, 750 ml of a yellowish brown transparent rice extract solution was obtained in the same manner as in Production Example 2 except that purified water / 1,2-pentanediol = 95/5 was used instead of purified water. Solid content concentration: 0.62%).
[0028]
Production Example 7 Preparation of Rice Extract Solution (6)
In Production Example 2, 700 ml of a yellowish brown transparent rice extract solution was obtained in the same manner as in Production Example 2 except that Koshihikari seedling leaves were used instead of Koshihikari leaves just before heading (solid content concentration: 0 .89%).
[0029]
Production Example 8 Preparation of Rice Extract Powder 600 ml of rice extract solution obtained in the same manner as in Production Example 2 was concentrated to 60 ml and freeze-dried to obtain 10.1 g of rice extract powder.
[0030]
Example 1 Cream [Component A] Part Liquid Paraffin 5.0
Hexalan (Note 1) 4.0
Paraffin 5.0
Glyceryl monostearate 2.0
Polyoxyethylene (20) sorbitan monostearate 6.0
Butylparaben 0.1
(Note 1) Technoble Co., Ltd. glyceryl trioctanoate [component B]
Extract solution of Production Example 2 5.0
Glycerin 5.0
Carboxymethyl monostearate 0.1
Methylparaben 0.1
Moiston C (Note 2) 1.0
Purified water Amount of 100 parts (Note 2) NMF component [C component] manufactured by Technoble Co., Ltd.
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 degreeC, C component was added, and also it stirred and mixed and prepared the cream.
[0031]
Example 2 Emulsion [component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Extract solution of Production Example 4 5.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Purified water Amount of 100 parts [component C]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to prepare an emulsion.
[0032]
Example 3 Lotion [component] part Extract solution of Production Example 5 5.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Fragrance Appropriate amount Purified water An amount that makes the total amount 100 parts The above ingredients were mixed to prepare a lotion.
[0033]
Example 4 Pack [ingredient] part polyvinyl alcohol 15.0
Hydroxymethylcellulose 5.0
Propylene glycol 5.0
Ethanol 10.0
Methylparaben 0.2
Extract solution of Production Example 6 5.0
Fragrance Appropriate amount Purified water An amount that makes the total amount 100 parts The above ingredients were mixed to prepare a pack.
[0034]
Example 5 Pressed Powder [Component A] Part Bengala 0.5
Yellow iron oxide 1.5
Black iron oxide 0.1
Titanium oxide 10.0
Nylon powder 4.0
Amount that makes 100 parts of sericite Mica 23.0
Talc 25.0
Extract powder of Production Example 8 0.05
[B component]
Squalane 1.0
Methylpolysiloxane 4.0
Propylparaben 0.1
Dehydroacetic acid 0.1
Liquid paraffin 2.0
Fragrance Appropriate Amounts The above A component and B component were mixed and stirred, mixed, then passed through a 200 mesh Tyler mesh sieve, and the resulting mixed powder was cast into a mold to prepare a uniform press powder.
[0035]
Example 6 Lipstick [Component A] Part Castor oil Amount that makes the total amount 100 parts Octyldodecanol 5.0
Lanolin 5.0
Liquid lanolin 5.0
Beeslow 4.0
Ozokerite 7.0
Candelilla Row 2.0
Carnavalou 1.0
[B component]
Titanium oxide 1.0
Dye 4.0
Extract powder of Production Example 8 0.1
[C component]
Fragrance A trace amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and rapidly cooled to prepare a lipstick.
[0036]
Example 7 Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben appropriate amount [B component]
Extract solution of production example 7 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methyl paraben Appropriate amount Purified water Amount that makes the total amount 100 parts [Component C]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above-mentioned C component was added, poured into a mold, and stirred until room temperature was reached to prepare a liquid foundation.
[0037]
Example 8 Treatment [Component] Part Stearyltrimethylammonium chloride 6.0
Polyvinylpyrrolidone 4.0
Glycerin 1.0
Ethylparaben 0.1
Extract solution of Production Example 2 5.0
Purified water A total amount of 100 parts The above ingredients were heated to 80 ° C. and then mixed and stirred to prepare a treatment.
[0038]
Example 9 Cream A cream was prepared in the same manner as in Example 1 except that the rice extract solution of Production Example 7 was used instead of the rice extract solution of Production Example 2.
[0039]
Comparative Example 1 Cream In Example 1, 0.1 part of α-tocopherol, which is an existing anti-aging ingredient (antioxidant), was used instead of 5.0 parts of the rice extract solution of Production Example 2. A cream was prepared in the same manner as in Example 1.
[0040]
Comparative Example 2 Cream In Example 1, instead of using 5.0 parts of the rice extract solution in Production Example 2, 5.0 parts of placenta extract, which is an existing skin aging prevention component (cell activator), was used. A cream was prepared in the same manner as in Example 1.
[0041]
Test Example 1 Antioxidant Action of Rice [Sample]
(1) Liquid obtained by diluting the rice extract solution of Production Example 2 with purified water to 5.0% (2) Liquid obtained by diluting the rice extract solution of Production Example 2 with purified water to 10.0% (3) Liquid obtained by diluting the rice extract solution of Production Example 7 with purified water to 5.0% (4) Liquid obtained by diluting the rice extract solution of Production Example 7 with purified water to 10.0% [0042]
[Test method]
An evaluation solution was prepared by mixing 1.0 ml of a 500 mM linoleic acid ethanol solution, 10 ml of 0.2N phosphate buffer (pH 6.98), 9.0 ml of ethanol, and 5.0 ml of a sample solution, and kept at 40 ° C. When the evaluation solution was prepared on the 0th day, the amount of lipid peroxide produced on the 3rd and 7th days was measured using the rodane iron method. As a control, the same test was carried out on a liquid obtained by mixing 5.0 ml of purified water (blank) or 5.0 ml of 40 μM tocopherol aqueous solution (positive control) instead of 5.0 ml of the sample solution. .
[0043]
[result]
The results are shown in Table 1.
[Table 1]

[0044]
As is apparent from the results in Table 1, the extracts obtained from the rice leaves before heading and the leaves of rice seedlings remarkably suppress the formation of lipid peroxides and exhibit a strong antioxidant effect.
In addition, the dry leaf obtained by exposing the rice leaf before heading similar to manufacture example 1 to the sun for 30 days without performing a heat enzyme deactivation process, and the rice (Koshihikari) collected 10 days after heading When the lipid peroxidation inhibitory action was investigated about the rice extract solution prepared by performing the same process as manufacture 1 and 2, respectively, using fresh leaves, the amount of lipid peroxide produced on the 4th day was all in Production Example 2. Or it reached 5-10 times the rice extract of 7, and the tendency for the antioxidant power of rice to fall was observed by long-term storage or heading after rice collection.
[0045]
Test Example 2 Rice Fibroblast Activation Action [Sample]
(1) Rice extract solution of Production Example 2 (2) Rice extract solution of Production Example 7
[Test method]
Human dermis-derived fibroblasts NB1RGB (000824) were seeded at 1 × 10 ⁴ cells / well in a 96-well microplate containing 0.5% FCS-containing minimum essential medium, pre-cultured at 37 ° C. for 1 day, The sample solution was added to a concentration of 2.5% or 5.0%, and further cultured at 37 ° C. for 6 days. Next, 0.2% MTT was added to the cell treatment solution to which the medium was removed and the surfactant (TRITON X-100) was added, and maintained at 37 ° C., and then a microplate reader (Model 1450, Bio-Rad). MTT value was measured at a wavelength of 370-630 nm.
For comparison, the same test was performed when 50 mM glucose was added instead of the rice extract solution and when no sample was added (blank).
[0047]
[result]
The results are shown in FIG.
As shown in FIG. 1, the rice extract of this invention has the effect | action which enhances the MTT activity with respect to a fibroblast, and activates this cell.
[0048]
Test Example 3 Skin Irritation Evaluation Test The skin irritation of the rice extract was evaluated by a neutral red uptake test using cultured rabbit corneal cells (SIRC), which is an alternative method to the eye mucosa irritation test. For comparison, α-tocopherol, which is a known antioxidant, was evaluated in the same manner.
[0049]
[sample]
(1) Extract powder of production example 8 (2) α-tocopherol (comparative sample)
[0050]
[Test method]
SIRC was seeded at 5000 cells / well in a 96-well microplate, cultured in Eagle's minimum essential medium (MEM) containing 5% calf serum for 3 days, and then the sample was added to a concentration of 0.2%. The culture was further continued for 2 days. Next, SIRC was cultured in Eagle MEM medium containing 0.05% neutral red (NR) for 3 hours, and the NR uptake rate was determined for the SRIC obtained here, and the skin irritation of each sample was evaluated from the value. .
[0051]
[result]
The results are shown in Table 2.
[Table 2]

[0052]
As shown in Table 2, the rice extract of the present invention is less irritating to the skin than existing antioxidants and can provide a cosmetic with excellent safety.
[0053]
Test Example 4 Monitor Test For the creams of Examples 1 and 9 and Comparative Examples 1 and 2, the effect on the skin was examined by a monitor test.
[Test method]
Randomly extracted 40 women aged 18-50 were divided into four groups of 10 subjects each, and each group was assigned one of the four combinations of the creams of the example and the comparative example. After applying the cream of Example or Comparative Example twice a day (morning, evening) for 1 month on the cheeks of the skin, the skin dullness, the improvement effect on the spots, the improvement effect on the fine wrinkles and the skin The improvement effect on beam and gloss was evaluated based on the following evaluation criteria.
[0054]
[Evaluation criteria]
(Improves skin dullness and stains)
A: Very improved B: Much improved C: Slightly improved D: No change E: On the contrary, it became more conspicuous (improvement effect on small wrinkles)
A: Almost inconspicuous B: Very inconspicuous C: Slightly inconspicuous D: Unchanged E: On the contrary (Improvement effect on skin elasticity and gloss)
A: Clearly improved B: Significantly improved C: Slightly improved D: Unchanged E: On the contrary it got worse
[result]
The results are shown in Table 3.
[0056]
[Table 3]

[0057]
As shown in Table 3, the cream containing the rice extract of the present invention exhibits superior skin aging prevention effect and rough skin improvement effect over conventional skin aging prevention cosmetics.
[0058]
【The invention's effect】
Formulation of the extract or Ranaru present invention leaf rice leaf extracts rice combines antioxidant and fibroblast activation action, they act upon skin application acts synergistically result, the skin It is extremely effective in preventing aging and improving rough skin. For this reason, the cosmetics of this invention containing this compounding agent show the skin aging prevention effect and the rough skin improvement effect which were improved more compared with the conventional anti-aging cosmetics. Also, distribution material mixture of the present invention has less irritation to the skin since they are derived from natural products, therefore cosmetics blended with this is excellent in safety.
[Brief description of the drawings]
FIG. 1 is a graph showing the MTT value of each sample of Test Example 2 (vertical axis: MTT value).

Claims (5)

Extraction of leaves of rice (Oryza sativa) with a solvent selected from water, lower alcohols, polyhydric alcohols, a mixture of water and lower alcohols, and a mixture of water and polyhydric alcohols An antioxidant and fibroblast activator comprising as an active ingredient an extract that exhibits oxidation and fibroblast activation. The agent according to claim 1, wherein the rice leaf is a rice leaf that has been subjected to enzyme deactivation treatment before collection and withdrawal color. The agent according to claim 2, wherein the enzyme deactivation treatment is performed by steaming heat treatment in which rice is brought into contact with steam. The agent according to any one of claims 1 to 3, wherein rice leaves before heading are used as rice leaves. A cosmetic comprising the antioxidant and the fibroblast activator according to any one of claims 1 to 4 .

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