JP3818998B2 - Whitening cosmetics - Google Patents

Description

  The present invention relates to a whitening cosmetic. More particularly, the present invention relates to a whitening cosmetic that has an excellent whitening effect and can be suitably used for basic cosmetics, makeup cosmetics, bath preparations, and the like.

  Conventionally, various researches have been conducted and various whitening cosmetics have been proposed as the need for cosmetics having a whitening effect increases. However, none of the conventional whitening cosmetics satisfy the whitening effect in particular in addition to the safety to the skin and the storage stability.

  Therefore, as a result of intensive research in view of the above-mentioned conventional techniques, the present inventors have reduced the tyrosinase activity of cultured pigment cells by an extract obtained by extracting cereals and residues generated during grain purification with a neutral medium. The present inventors have found that the present invention has an excellent effect of suppressing the formation of melanin, suppressing pigmentation caused by ultraviolet irradiation, and improving dullness.

  That is, the present invention relates to a whitening cosmetic comprising a decomposition product obtained by treating a cereal or an extract obtained by extracting a cereal purified residue with a neutral medium with an enzyme.

  Extracts obtained by extracting cereals and cereal refined residues with neutral media reduce the intracellular tyrosinase activity of cultured pigment cells, which is an indicator of the whitening effect, with little inhibition of cellular activity. Since it exhibits excellent effects such as suppressing the formation of melanin and suppressing pigmentation caused by UV irradiation, whitening cosmetics formulated with such an extract will show spots and freckles due to the accumulation of melanin. It has the effect of suppressing, improving the skin condition, and maintaining white and beautiful skin with improved dullness.

  Furthermore, the whitening cosmetic composition of the present invention in which a decomposition product obtained by treating the extract with an enzyme is blended exhibits a more excellent whitening effect.

  In addition, the whitening cosmetic of the present invention has excellent whitening effects as described above, and also has excellent effects such as safety on the skin and storage stability.

  As described above, the whitening cosmetic composition of the present invention is a blend of a decomposition product obtained by treating a cereal or an extract obtained by extracting a cereal refined residue with a neutral medium with an enzyme.

  Examples of grains used in the present invention include rice and wheat. Examples of cereal refining residues include rice bran, bran, corn refining residue, and soybean refining residue. Among these, the whitening effect obtained by extracting with a neutral medium described below is superior. Rice bran and bran are preferable in terms of

  Examples of the solvent used when extracting the cereal and the cereal refined residue with a neutral medium include water such as purified water; monovalent lower alcohols such as ethanol; oleyl alcohol, stearyl alcohol, octyldodecanol, and the like. Monovalent higher alcohols; polyols such as ethylene glycol, propylene glycol, glycerin and 1,3-butylene glycol; ketones such as acetone; esters such as ethyl acetate; hydrocarbon solvents such as hexane, chloroform and benzene These may be used alone or in combination of two or more. Among these, purified water or a mixed solvent of purified water and one or more of ethanol, glycerin and 1,3-butylene glycol is preferable because it can be widely applied to cosmetics. .

  In the case of using the mixed solvent, for example, in the case of a mixed solvent of purified water and ethanol, the volume ratio of both is 1: 1 to 25: 1, and the mixed solvent of purified water and glycerin is used. Unfortunately, the volume ratio of both is 1: 1 to 15: 1, and in the case of a mixed solvent of purified water and 1,3-butylene glycol, the volume ratio of both is 1: 1 to 15: 1. It is preferable.

  In the present invention, when extracting a grain or grain refined residue with a neutral medium, the pH of the extraction solution containing the grain or grain refined residue may be about 5 to 9, and the solvent is used as it is. For example, alkaline solvents such as sodium salts such as sodium hydroxide and sodium carbonate, potassium salts such as potassium hydroxide, and acid regulators such as citric acid, hydrochloric acid, phosphoric acid and sulfuric acid may be used as the solvent. It can also mix | blend and adjust so that it may become the target pH. Among these regulators, sodium hydroxide, sodium carbonate, hydrochloric acid, and phosphoric acid are preferred because they can be adjusted to a target pH at a low concentration.

  The time required for the extraction treatment varies depending on the type of solvent used, the target pH, the extraction temperature, etc., and thus cannot be determined unconditionally. For example, when the pH is 5 to 9, it is usually 6 hours at room temperature. It is preferably about 7 days, especially about 12 to 48 hours. In addition, extraction temperature becomes like this. Preferably it is about 4-40 degreeC, More preferably, it is about 10-30 degreeC.

  The extract thus obtained may be directly blended into whitening cosmetics, for example, it may be blended after concentration under reduced pressure to adjust the concentration, or powdered by, for example, freeze drying or spray drying. You may mix what you did. However, these cases are not included in the present invention.

  The blending amount of the extract differs depending on the type of the whitening cosmetic product to be used and cannot be determined unconditionally. However, if the blending amount is too small, the extract is blended. Since there is a tendency that the whitening effect is not sufficiently exhibited, the whitening cosmetic is adjusted so that it is 0.0005 parts or more in terms of solid content, especially 0.005 parts or more with respect to 100 parts (parts by weight). It is preferable that if the amount is too large, it tends to be technically difficult to stably mix the extract with a whitening cosmetic. It is preferable to adjust to 5 parts or less, especially 1 part or less.

  In the whitening cosmetic composition of the present invention, the extract is treated with an enzyme because the whitening effect such as reduction of tyrosinase activity, suppression of melanin production, and suppression of pigmentation caused by ultraviolet irradiation is greater. The resulting degradation product is blended.

  Examples of the enzyme include actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, peptidases such as glycylglycine peptidase, carboxypeptidase, and aminopeptidase. And proteolytic enzymes such as bromelain. Among these, whitening makeup in which a combination of actinase and at least one proteolytic enzyme selected from pepsin, trypsin, papain, peptidase and bromelain is combined with the resulting degradation product. The material is preferable from the viewpoint of excellent storage stability and safety, and a combination of actinase, pepsin and trypsin is particularly preferable.

  In the present invention, when two or more kinds of enzymes are used for treatment, one kind of enzyme is usually used at a time.

  The amount of enzyme used per time when the enzyme treatment is performed is about 0.0005 to 0.05 parts per 100 parts of the neutral extraction solution containing the grain or the refined residue of the grain, especially 0.001. It is about -0.005 part, and it is preferable from the point of the effect of this enzyme that it is about 0.003-0.015 part in total.

  The time required for the enzyme treatment varies depending on the type of enzyme used, the decomposition temperature, and the like, and thus cannot be determined unconditionally, but is usually about 30 minutes to 24 hours for one type of enzyme, especially about 1 to 4 hours. It is preferable. In addition, the decomposition temperature of the exemplified enzyme is about 30 to 50 ° C.

  Further, when performing the enzyme treatment, the pH of the extraction solution may be adjusted to the optimum pH of the enzyme used. To adjust the pH of the extraction solution, for example, the extraction may be performed as necessary. An acidic adjuster, an alkaline adjuster, or the like used in carrying out can be used.

  The decomposition product thus obtained may be directly blended into whitening cosmetics, for example, it may be blended after concentration under reduced pressure to adjust the concentration, or powdered by, for example, freeze drying or spray drying You may mix things.

  In addition, it is preferable that the solution containing the obtained degradation product is adjusted to pH 4-8 from the point of the safety | security to skin.

  The amount of the decomposed product varies depending on the type of the targeted whitening cosmetic, and therefore cannot be determined unconditionally. However, if the amount is too small, the decomposition product is blended. Since there is a tendency that the whitening effect is not sufficiently exhibited, it is preferable to adjust the amount to be 0.0005 part or more, especially 0.005 part or more in terms of solid content with respect to 100 parts of the whitening cosmetic composition, and too much. In many cases, there is a tendency that it is technically difficult to stably mix the decomposed product with the whitening cosmetic, so 5 parts or less in terms of solid content with respect to 100 parts of the whitening cosmetic, especially 1 part. It is preferable to adjust to be as follows.

  The degradation product and the extract used in the present invention simultaneously exhibit a decrease in tyrosinase activity of cultured pigment cells, a suppression effect of melanin production, and a suppression effect of pigmentation caused by ultraviolet irradiation. In the case of using the whitening cosmetic composition of the present invention, the expression of spots and freckles due to the accumulation of melanin is suppressed, and white and beautiful skin with improved dullness is maintained.

  The whitening cosmetic composition of the present invention, as described above, is a mixture of a decomposition product obtained by treating an extract obtained by extracting grains or a refined residue of grains with a neutral medium with an enzyme. (The above extract can be used in place of the decomposed product, but this is not included in the whitening cosmetics of the present invention). In the present invention, in addition to these, for example, generally in cosmetics. Including excipients and fragrances used, fats and oils, surfactants, moisturizers, whitening agents, pH adjusters, thickeners, preservatives, antioxidants, ultraviolet absorbers, pigments, cleaning agents, Various cosmetic ingredients such as desiccants and emulsifiers can be appropriately blended into the whitening cosmetic.

  The fats and oils are generally used in cosmetics, for example, liquid oils such as liquid paraffin, paraffin, cetanol, avocado oil, olive oil, jojoba oil, coconut oil; beef fat, pork fat, horse fat, turtle oil, Animal fats and oils such as mink oil, perserine oil, and squalane; and synthetic fats and oils such as methylpolysiloxane, behenyl alcohol, glyceryl tricaprycaprate, glyceryl trioctanoate, glycerin triisopalmitate, and silicone oil.

  Examples of the surfactant include anionic surfactants such as sodium lauryl sulfate, triethanolamine lauryl sulfate, and diethanolamide lauric acid; cationic properties such as stearyltrimethylammonium chloride, cetyltrimethylammonium chloride, and benzalkonium chloride Surfactants: Nonionic surfactants such as glyceryl monostearate, sorbitan monostearate, polyoxyethylene (20) sorbitan monostearate, polyoxyethylene hydrogenated castor oil, sucrose ester, fatty acid amide, etc. .

  Examples of the humectant include synthetic humectants such as glycerin, propylene glycol, 1,3-butylene glycol, sodium pyrrolidone carboxylate, pantothethein-S-sulfonate; hyaluronic acid, collagen, elastin, placenta extract, royal jelly, Examples include microbial fermentation liquids such as chitin, chitosan, pectin, and other natural moisturizing liquids such as extracts derived from other plants and animals.

  Examples of the whitening agent include kojic acid, ascorbic acid, arbutin, placenta extract and derivatives thereof, and other plant and animal extracts.

  Examples of the pH adjusting agent include organic acids such as citric acid and sodium citrate, and salts thereof.

  Examples of the thickener include carboxymethylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, carboxyvinyl polymer, polyvinyl alcohol, gum tragacanth, sodium alginate, carrageenan and the like.

  Examples of the preservative include paraoxybenzoic acid esters such as methyl paraben, ethyl paraben, propyl paraben, and butyl paraben, phenoxyethanol, ethanol, and dehydroacetic acid.

  Examples of the antioxidant include vitamin E, butyloxytoluene (BHT), butyloxyanizole (BHA), and the like.

  Examples of the pigment include bengara, yellow iron oxide, black iron oxide, titanium oxide, nylon powder, sericite, mica, and talc.

  Examples of the cleaning agent include sodium lauryl sulfate.

  Examples of the emulsifier include soybean lecithin oil.

  Examples of the excipient include sodium sulfate.

  The blending amount of each of these cosmetic ingredients varies depending on the intended use of the whitening cosmetic, and therefore cannot be determined unconditionally, and is preferably adjusted as appropriate according to the intended use.

  The form of the whitening cosmetic composition of the present invention is arbitrary and is not particularly limited. However, the whitening cosmetic composition of the present invention prevents the appearance of dull skin, stains and freckles, is youthful, healthy, and dull. It has excellent properties such as maintaining an improved white and beautiful skin condition, such as basic cosmetics such as creams, emulsions, lotions, essences, face wash, packs, lipsticks, foundations, liquid foundations, press powders, etc. It can be used as toiletries such as makeup cosmetics, body soap, soap.

  Furthermore, when the extract or decomposition product and these dry powders are put into hot water, skin whitening cosmetics are effective for improving the skin condition through percutaneous absorption. Can be used. Thus, when using whitening cosmetics as a bath preparation, the amount of the extract or decomposition product added to the whitening cosmetics, considering the effect of improving the skin condition produced by the extract or decomposition product, It is preferably 0.0005 to 5.0 parts, especially 0.001 to 0.1 parts in terms of solid content of the extract or decomposition product with respect to 100 parts of the whitening cosmetic. In addition, when using the said bath agent, it is preferable to adjust the usage-amount of this bath agent so that a bath agent may be about 5-50g with respect to 200 liters of normal hot water.

  Next, the whitening cosmetic composition of the present invention will be described in more detail based on examples, but the present invention is not limited to such examples.

Preparation Example 1 (Manufacture of rice neutral extract)
200 g of rice is added to 800 ml of purified water, the pH is adjusted to 6.0 to 8.0 with a 0.1N aqueous sodium hydroxide solution, and extracted by immersion for about 24 hours at room temperature. About 480 ml was obtained.

Preparation Example 2 (Production of enzymatic degradation product of rice neutral extract)
To the extract obtained in Preparation Example 1, 5 mg of actinase (optimum pH 8.0) was added and treated at 30 to 40 ° C. over 1 to 2 hours, and then 5 mg of pepsin (optimum pH 2.0). Was added and treated at 30 to 40 ° C. over 1 to 2 hours, and finally 5 mg of trypsin (optimum pH 8.0) was added and treated at 30 to 40 ° C. over 1 to 2 hours.

  In addition, when adding each enzyme, it adjusted so that an extract might become optimal pH of each enzyme.

  This was filtered to obtain about 250 ml of a pale yellow transparent enzyme degradation product solution (solid content: about 2.0% by weight).

Preparation Example 3 (Manufacture of rice bran neutral extract)
In Preparation Example 1, about 480 ml of an extract (solid content: about 1.5% by weight) was obtained in the same manner as in Preparation Example 1, except that rice bran was used instead of rice.

Preparation Example 4 (Manufacture of enzymatic degradation product of rice bran neutral extract)
In Preparation Example 2, a pale yellow transparent enzyme degradation product solution (solid content) was prepared in the same manner as in Preparation Example 2 except that the extract obtained in Preparation Example 3 was used instead of the extract obtained in Preparation Example 1. About 2.0% by weight) about 250 ml.

Preparation Example 5 (Production of enzyme-decomposed product of rice bran neutral extract)
In Preparation Example 4, a pale yellow transparent enzyme degradation product solution (solid content: about 2.0% by weight) was prepared in the same manner as Preparation Example 4 except that papain (optimum pH 7.0) was used instead of pepsin. 250 ml was obtained.

Preparation Example 6 (Production of enzymatic decomposition product of bran neutral extract)
200 g of bran was added to 800 ml of purified water, the pH was adjusted to 6.0 to 8.0 with 0.1 N hydrochloric acid, and extraction was performed by immersion for about 24 hours at room temperature to obtain about 480 ml of an extract.

  The obtained extract was treated in the same manner as in Preparation Example 4 except that 10 mg each of actinase, pepsin, and trypsin were sequentially added.

  This was filtered to obtain about 300 ml of a pale yellow transparent enzyme degradation product solution (solid content: about 2.0% by weight).

Preparation Example 7 (Production of freeze-dried processed rice bran neutral extract)
100 g of the extract obtained in Preparation Example 3 was concentrated and then freeze-dried in vacuo to obtain about 1.5 g of a dry powder of the extract.

Preparation Example 8 (Production of spray-dried product of enzyme-decomposed product of rice bran neutral extract)
The enzyme degradation product solution 250 g obtained in Preparation Example 4 was spray-dried to obtain about 5 g of a dry powder of the degradation product.

  Next, the following tests were conducted using the extract obtained in Preparation Example 3 and the enzyme degradation product solution obtained in Preparation Example 4.

Test Example 1 (Inhibition of intracellular tyrosinase activity)
Cultured B16 mouse melanoma cells were seeded at 8000 cells / well in a 96-well microplate (CORNING), and were cultured in Eagle's minimal essential medium (MEM) containing 3% by volume of calf serum at 37 ° C. and 5% CO ₂ for 24 hours. After pre-culture, the extract obtained in Preparation Example 3 or the enzyme degradation product solution obtained in Preparation Example 4 was replaced with Eagle MEM containing 3% by volume calf serum added with 5% by volume or 10% by volume. ° C., and cultured for 48 hours under 5% CO _2.

  Next, after removing the medium and washing with PBS (−), 0.1N phosphate buffer and 5 mmol L-dopa were added and incubated at 37 ° C. for 1 hour to determine the amount of dopachrome produced by a microplate reader ( BIO RAD).

  FIG. 1 (when using the extract obtained in Preparation Example 3) and FIG. 3 (as obtained in Preparation Example 4) show the relationship between the added amount of the extract or the added amount of the enzyme degradation product solution and the amount of dopachrome produced. In the case of using an enzyme degradation product solution), the graph is shown. The graphs of FIGS. 1 and 3 show the amount of dopachrome produced when no extract or enzyme degradation product solution was added at all, as 100%.

  In addition, the amount of the extract added or the amount of the enzyme degradation product solution and the amount of reduced nicotinamide adenosine dinucleotide (hereinafter referred to as NADH) in mitochondria by the MTT reduction method representing the cell activity of cultured B16 mouse melanoma cells FIG. 2 (when using the extract obtained in Preparation Example 3) and FIG. 4 (when using the enzyme degradation product solution obtained in Preparation Example 4) are shown in the graphs of FIG. The graphs of FIGS. 2 and 4 show the amount of NADH in mitochondria as 100% by the MTT reduction method when no extract or enzyme degradation product solution is added.

  As is clear from the graphs shown in FIG. 1 and FIG. 2, the extract obtained in Preparation Example 3 hardly inhibited the cell activity of cultured B16 mouse melanoma cells as the amount added increased. It turns out that the production amount of dopachrome is reduced and the intracellular tyrosinase activity is suppressed.

  Moreover, as is apparent from the graphs shown in FIGS. 3 and 4, the enzyme degradation product solution obtained in Preparation Example 4 inhibits the cell activity of cultured B16 mouse melanoma cells as the amount added increases. It can be seen that the production amount of dopachrome is remarkably reduced and the intracellular tyrosinase activity is remarkably suppressed.

Test Example 2 (melanin production inhibitory action)
Preparation Example 4 after culturing 10,000 B16 mouse melanoma cells in a petri dish (CORNING) having an inner diameter of 60 mm and pre-culturing with Eagle MEM containing 5% by volume calf serum at 37 ° C. and 5% CO ₂ for 2 days. The obtained enzyme degradation product solution was replaced with 3% by volume calf serum-containing Eagle MEM added with 5% by volume or 10% by volume, and further cultured at 37 ° C. under 5% CO ₂ for 3 days.

  Next, after removing the medium and washing with PBS (-), detaching the cells with trypsin, counting the number of cells, and simultaneously heat-treating with 1N aqueous sodium hydroxide containing 10% dimethyl sulfoxide to elute melanin. The amount of melanin produced was measured for the solution using a variable spectrophotometer (U-2000, manufactured by Hitachi, Ltd.).

FIG. 5 is a graph showing the relationship between the amount of the enzyme degradation product solution added and the amount of melanin produced per 10 ⁶ cells. In addition, the graph of FIG. 5 represents the production amount of melanin when adding no enzyme decomposition product solution as 100%.

  As is clear from the graph shown in FIG. 5, it can be seen that the enzyme degradation product solution obtained in Preparation Example 4 remarkably suppresses the production of melanin in cultured B16 mouse melanoma cells as the amount added increases. .

Test Example 3 (Skin safety)
Using the enzyme degradation product solutions obtained in Preparation Examples 2 and 4, safety against human skin was examined by performing a closed patch test.

  Twenty healthy adult males and females aged 20-70 years randomly extracted were used as subjects, and 0.2 ml of the enzyme degradation product solution obtained in Preparation Example 2 or Preparation Example 4 was applied to the upper arm skin of each subject. Then, an adhesive bandage for human patch test (manufactured by River Tape Co., Ltd.) was attached from the upper part.

  Further, as a control, the human patch test adhesive bandage was affixed so that the circular fabric portion was parallel to the site where the enzyme degradation product solution was applied.

  After 48 hours, remove the adhesive patch for each human patch test, visually observe the skin symptoms at the site where the enzyme degradation product solution was applied and the control site, and based on the evaluation criteria of the closed patch test by the Japan Patch Test Society And evaluated.

  As a result, no temporary irritation to the human skin of the enzyme degradation product solutions obtained in Preparation Examples 2 and 4 was observed, and it was found that all of these enzyme degradation product solutions were excellent in safety to the skin. .

Formulation Examples 1-12 and Comparative Formulation Examples 1-3
Formulation Example 1 (Cream)
[(A) component] (part)
Liquid paraffin 5.0
Hexalan (glyceryl trioctanoate, manufactured by Technoble Co., Ltd.) 4.0
Paraffin 5.0
Cetanol 2.0
Glyceryl monostearate 2.0
Polyoxyethylene (20) sorbitan monostearate 6.0
Butylparaben 0.1
[Component (B)]
Extract liquid obtained in Preparation Example 10.0
Glycerin 5.0
Sodium carboxymethylcellulose 0.1
Methylparaben 0.1
Moiston C (Natural Moisturizing Factor, manufactured by Technoble Co., Ltd.) 1.0
Purified water The total amount is 100.0 parts [component (C)]
Fragrance 0.3
The components (A) and (B) were each heated to 80 ° C. or higher, and then the components (A) and (B) were mixed and stirred. After cooling this to 50 degreeC, the said (C) component was added, and it further stirred and mixed and prepared the uniform cream.

Formulation Example 2 (Cream)
A cream was prepared in the same manner as in Formulation Example 1, except that the enzyme degradation product solution obtained in Preparation Example 2 was used instead of the extract obtained in Preparation Example 1.

Formulation Example 3 (Cream)
A cream was prepared in the same manner as in Formulation Example 1 except that the extract obtained in Preparation Example 3 was used instead of the extract obtained in Preparation Example 1.

Formulation Example 4 (Cream)
A cream was prepared in the same manner as in Formulation Example 1 except that the enzyme degradation product solution obtained in Preparation Example 4 was used instead of the extract obtained in Preparation Example 1.

Formulation Example 5 (Emulsion)
[(A) component] (part)
Liquid paraffin 6.00
Hexalan (glyceryl trioctanoate, manufactured by Technoble Co., Ltd.) 4.00
Jojoba oil 1.00
Polyoxyethylene (20) sorbitan monostearate 2.00
Soy lecithin oil 1.50
Methylparaben 0.15
Ethylparaben 0.03
[Component (B)]
Extract obtained in Preparation Example 1 30.00
Glycerin 3.00
1,3-butylene glycol 2.00
Sodium carboxymethylcellulose 0.30
Sodium hyaluronate 0.01
Amount of purified water totaling 100.00 parts [component (C)]
Perfume 0.05
The components (A) and (B) were each heated to 80 ° C., and then the components (A) and (B) were mixed and stirred. After cooling this to 50 degreeC, the said (C) component was added and further stirred, and the uniform emulsion was prepared.

Formulation Example 6 (Lotion)
[Components] (Part)
L-ascorbyl magnesium phosphate 2.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Water-soluble placenta extract 1.0
Extract liquid obtained in Preparation Example 10.0
Fragrance Trace amount Purified water Amount to make the total amount 100.0 parts The above ingredients were mixed to prepare a uniform lotion.

Formulation Example 7 (Essence)
[Components] (Part)
Ethanol 10.0
Glycerin 5.0
1,3-butylene glycol 1.0
Oleyl alcohol 0.1
Polyoxyethylene sorbitan monolaurate (20EO) 1.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.3
Enzymatic degradation product solution obtained in Preparation Example 5 1.0
Perfume Trace amount Water-soluble collagen 1.0
Purified water A total amount of 100.0 parts The above ingredients were mixed to prepare a uniform essence.

Formulation example 8 (pack)
[Components] (Part)
Polyvinyl alcohol 15.0
Hydroxymethylcellulose 5.0
Propylene glycol 5.0
Ethanol 10.0
Methylparaben 0.1
Enzymatic degradation product solution obtained in Preparation Example 10.0
Perfume Small amount Purified water Amount that the total amount is 100.0 parts The above ingredients were mixed to prepare a uniform pack.

Formulation Example 9 (face wash)
[Components] (Part)
Stearic acid 15.0
Lauric acid 5.0
Myristic acid 15.0
Glyceryl monostearate 4.0
Potassium hydroxide 7.0
Glycerin 8.0
Extract liquid obtained in Preparation Example 10.0
Methylparaben 0.2
Purified water A total amount of 100.0 parts The above ingredients were heated to 85 ° C. and mixed to prepare a uniform facial cleanser.

Formulation Example 10 (Bath Agent)
[Components] (Part)
Sodium sulfate 35.0
Sodium bicarbonate 52.0
Borax 2.0
Sodium carboxymethylcellulose 1.0
Red No. 201 Trace amount Fragrance amount Trace amount of freeze-dried product obtained in Preparation Example 7 Total amount of 100.0 parts The above ingredients were mixed to prepare a uniform bath preparation.

Formulation Example 11 (lipstick)
[(A) component] (part)
Castor oil 50.0
Octyldodecanol 5.0
Lanolin 5.0
Liquid lanolin 5.0
Beeslow 4.0
Ozokerite 7.0
Candelilla Row 2.0
Carnavalou 1.0
[Component (B)]
Titanium oxide 1.0
Dye (red 201 etc.) Total 4.0
The amount by which the total amount of the spray-dried product obtained in Preparation Example 8 is 100.0 parts [component (C)]
Fragrance A trace amount After the components (A) and (B) were heated, the components (A) and (B) were mixed and stirred. This was reheated, the component (C) was added, poured into a mold, and rapidly cooled to prepare a lipstick.

Formulation Example 12 (Liquid Foundation)
[(A) component] (part)
Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben appropriate amount [Component (B)]
Enzyme degradation product solution obtained in Preparation Example 4 Total amount of 100.0 parts Carboxymethylcellulose sodium 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben appropriate amount [component (C)]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components (A) and (B) were heated, and then the components (A) and (B) were mixed and stirred. This was reheated, the component (C) was added, poured into a mold, and stirred until it reached room temperature to prepare a liquid foundation.

Comparative formulation example 1 (cream)
A cream was prepared in the same manner as in Formulation Example 1 except that purified water was used in place of the extract obtained in Preparation Example 1.

Comparative formulation example 2 (bath preparation)
A bath preparation was prepared in the same manner as in Preparation Example 10 except that mannitol (D-mannitol) was used instead of the freeze-dried product obtained in Preparation Example 7.

Comparative Formulation Example 3 (Liquid Foundation)
A liquid foundation was prepared in the same manner as in Formulation Example 12 except that purified water was used in place of the enzyme degradation product solution obtained in Preparation Example 4.

Reference example 1
Using the creams obtained in Formulation Example 1 and Comparative Formulation Example 1, the inhibitory effect on pigmentation due to ultraviolet irradiation was examined.

  Ten healthy adult males aged 20-35 years randomly extracted were used as subjects, and two 1 cm × 1 cm ultraviolet irradiation portions were set on the inner side of the forearm. Using a UV-B lamp (manufactured by Toshiba Corporation, FL20-SE), an amount of ultraviolet rays corresponding to the minimum amount of erythema (MED) of each subject measured in advance was once a day (morning) for 3 days. Irradiated continuously.

  For 30 consecutive days from the start of UV irradiation, the UV irradiation period (the first 3 days) is immediately after UV irradiation and twice a day in the evening, and after the UV irradiation period has passed (after the 4th day), it is one day in the morning and evening. About 0.01 g of the cream obtained in Formulation Example 1 and the cream obtained in Comparative Formulation Example 1 were applied twice to each ultraviolet irradiation part.

The pigmentation state of the ultraviolet irradiation part of each subject was visually observed every 5 days from the ultraviolet irradiation start date, and the pigmentation inhibitory effect when the cream obtained in Prescription Example 1 was applied was based on the following evaluation criteria. And evaluated.
(Evaluation criteria)
A: In the place where the cream obtained in Formulation Example 1 was applied, almost no pigmentation was observed.
B: Pigmentation is clearly less in the area where the cream obtained in Formulation Example 1 is applied than in the area where the cream obtained in Comparative Formulation Example 1 is applied.
C: Pigmentation is less in the area where the cream obtained in Formulation Example 1 is applied than in the area where the cream obtained in Comparative Formulation Example 1 is applied.
D: Pigmentation is slightly less at the place where the cream obtained in Formulation Example 1 is applied than at the place where the cream obtained in Comparative Formulation Example 1 is applied.

  The evaluation result of the pigmentation state every 5 days from the start date of ultraviolet irradiation is expressed by the ratio of the number of people who made each evaluation A to D when 10 subjects are 100%, and the change in the occupancy rate of each evaluation is illustrated. This is shown in the graph of FIG.

Example 1
In Reference Example 1, the pigmentation state of the ultraviolet irradiation part of each subject was evaluated in the same manner as in Reference Example 1 except that the cream obtained in Formulation Example 2 was used instead of the cream obtained in Formulation Example 1.

  The evaluation results of the pigmentation state every 5 days from the ultraviolet irradiation start date are shown in the graph of FIG.

  In Reference Example 1 and Example 1, when the cream obtained in Formulation Examples 1 and 2 was applied, there was no subject who had an abnormality on the skin.

  In addition, the cream obtained in Formulation Examples 1 and 2 did not change in its state in 30 days.

  As is clear from the results shown in FIG. 6 and FIG. 7, regardless of whether the cream obtained in Formulation Example 1 or the cream obtained in Formulation Example 2 was used, from the start date of ultraviolet irradiation. As the number of days elapses, the difference in the inhibitory action on pigmentation from the case of using the cream obtained in Comparative Formulation Example 1 increases, and the cream obtained in Formulation Examples 1 and 2 has excellent pigmentation. It turns out that there exists a suppression effect.

  Furthermore, comparing the graph of FIG. 6 with the graph of FIG. 7, when using the cream obtained in Prescription Example 2, the occupancy rate of A evaluation in which almost no pigmentation is observed is even higher. It can be seen that the enzyme degradation product solution obtained in Preparation Example 2 blended in the cream of Formulation Example 2 exhibits better pigmentation-inhibiting action.

Reference example 2
In Reference Example 1, the pigmentation state of the ultraviolet irradiation part of each subject was evaluated in the same manner as in Reference Example 1 except that the cream obtained in Formulation Example 3 was used instead of the cream obtained in Formulation Example 1.

  The evaluation results of the pigmentation state every 5 days from the start date of ultraviolet irradiation are shown in the graph of FIG.

Example 2
In Reference Example 1, the pigmentation state of the ultraviolet irradiation part of each subject was evaluated in the same manner as in Reference Example 1 except that the cream obtained in Formulation Example 4 was used instead of the cream obtained in Formulation Example 1.

  The evaluation results of the pigmentation state every 5 days from the ultraviolet irradiation start date are shown in the graph of FIG.

  In Reference Example 2 and Example 2, when the cream obtained in Formulation Examples 3 and 4 was applied, there was no subject who had an abnormality on the skin.

  In addition, the creams obtained in Formulation Examples 3 and 4 did not change in the state in 30 days.

  As is clear from the results shown in FIG. 8 and FIG. 9, regardless of whether the cream obtained in Formulation Example 3 or the cream obtained in Formulation Example 4 is used, from the start date of ultraviolet irradiation. As the number of days elapses, the difference in the inhibitory effect on pigmentation from the case of using the cream obtained in Comparative Formulation Example 1 increases, and the cream obtained in Formulation Examples 3 and 4 has excellent pigmentation. It turns out that there exists a suppression effect.

  Furthermore, comparing the graph of FIG. 8 with the graph of FIG. 9, when the cream obtained in Formulation Example 4 is used, the occupancy rate of A evaluation in which pigmentation is hardly observed is extremely high. It can be seen that the enzyme degradation product solution obtained in Preparation Example 4 blended in the cream of Formulation Example 4 exhibits a very excellent pigmentation inhibitory action.

Reference example 3
Using the bath preparations obtained in Formulation Example 10 and Comparative Formulation Example 2, the inhibitory action on skin pigmentation by bathing was examined.

A group of 20 healthy adult males and females aged 30-60 years randomly selected were taken as test subjects, and 25 g of each bath preparation was dissolved in 200 liters of hot water and bathed once a day for 1 month. The pigmentation state of the lower part of the eyelid of each subject group was visually observed and evaluated based on the following evaluation criteria. The results are shown in Table 1.
(Evaluation criteria)
A: Almost no pigmentation.
B: Pigmentation was clearly reduced.
C: Pigmentation was less than before using the bath preparation.
D: There is almost no change before using the bath preparation.
E: Pigmentation was increased more than before using the bath preparation.

  As is apparent from the results shown in Table 1, when the bath preparation obtained in Formulation Example 10 is used, the pigmentation is almost eliminated to less, so that the bath use obtained in Comparative Formulation Example 2 is used. It can be seen that the agent can hardly suppress pigmentation, whereas the bath preparation obtained in Formulation Example 10 has an excellent pigmentation inhibitory effect.

  In Reference Example 3, when taking a bath using the bath preparation obtained in Prescription Example 10, there was no subject who had an abnormality in the skin.

  Further, the state of the bath preparation obtained in Formulation Example 10 did not change during one month.

Example 3
Using the liquid foundation obtained in Formulation Example 12 and Comparative Formulation Example 3, the effect of improving dullness was examined.

40 healthy adult women aged 25-35, randomly selected, were used as subjects, and the liquid foundation obtained in Prescription Example 12 for each subject's right cheek was obtained in Comparative Prescription Example 3 for the left cheek. After applying the liquid foundation once a day for 1 month at normal usage (about 0.1 g), the left and right cheeks were visually observed and compared, based on the following criteria: And evaluated.
(Evaluation criteria)
A: The right cheek was duller and less dull.
B: The dullness was clearly less on the right cheek.
C: The right cheek was slightly duller.
D: There is no difference between the left and right cheeks.
E: The left cheek was clearly duller.

  As a result, 19 people with A evaluation, 20 people with B evaluation, and 1 person with C evaluation, there was no subject who made D evaluation and E evaluation, and the liquid foundation obtained in Prescription Example 12 was used. Ai has less dullness compared to the case where the liquid foundation obtained in Comparative Formulation Example 3 is used, so that the liquid foundation obtained in Formulation Example 12 has an excellent effect of improving dullness. I understand that.

  In Example 3, when the liquid foundation obtained in Formulation Example 12 was applied, there was no subject who had an abnormality on the skin.

  In addition, the liquid foundation obtained in Formulation Example 12 did not change in its state within one month.

It is a graph which shows the relationship between the addition amount of the extract obtained in the preparation example 3, and the production amount of dopachrome. It is a graph which shows the relationship between the addition amount of the extract obtained in Preparation Example 3, and the amount of NADH in mitochondria by MTT reduction method. It is a graph which shows the relationship between the addition amount of the enzyme decomposition product solution obtained in the preparation example 4, and the production amount of dopachrome. It is a graph which shows the relationship between the addition amount of the enzyme degradation product solution obtained in the preparation example 4, and the amount of NADH in mitochondria by MTT reduction method. ⁶ is a graph showing the relationship between the amount of enzyme degradation product solution obtained in Preparation Example 4 and the amount of melanin produced per 10 ⁶ cells. It is a graph which shows a time-dependent change from the ultraviolet irradiation start date of the occupation rate of each evaluation of the pigmentation inhibitory effect when the cream obtained in the prescription example 1 is applied. It is a graph which shows a time-dependent change from the ultraviolet irradiation start date of the occupation rate of each evaluation of the pigmentation inhibitory effect when the cream obtained in the prescription example 2 was applied. It is a graph which shows a time-dependent change from the ultraviolet irradiation start date of the occupation rate of each evaluation of the pigmentation inhibitory effect when the cream obtained in the prescription example 3 was applied. It is a graph which shows a time-dependent change from the ultraviolet irradiation start date of the occupation rate of each evaluation of the pigmentation inhibitory effect when the cream obtained in the prescription example 4 was applied.

Claims (3)

A whitening cosmetic comprising a decomposition product obtained by treating an extract obtained by extracting rice or rice bran with a neutral medium with an enzyme. Extraction with a neutral medium, pH 5 to 9, the extraction temperature 4 to 40 ° C., the extraction time is at room temperature for 6 hours to 7 days extraction claim 1 Symbol placement whitening cosmetics. The enzyme is (A) actinase,
(B) The whitening cosmetic composition according to claim 1, which is a combination of at least one proteolytic enzyme selected from the group consisting of pepsin, trypsin, papain, peptidase and bromelain.

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