KR102048973B1 - Skin-lightening Composition Using an Ginsenoside compound K and an Extract of Rice Bran - Google Patents

Abstract

The present invention discloses a composition for skin whitening using rice bran extract and ginsenoside compound K having tyrosinase inhibitory activity and melanin production inhibitory activity.

Description

Skin-lightening Composition Using an Ginsenoside compound K and an Extract of Rice Bran}

The present invention relates to a composition for skin whitening using ginsenoside compound K and rice bran extract.

With the development of medical technology and the improvement of living standard, people's desire for beauty is getting stronger and the appearance itself is becoming a social competitiveness, so the preference for skin whitening is increasing and the cosmetics market is expanding (J. Korean Soc. Food Sci.Nutr., 42 (3): 355 (2013)).

The skin is the largest organ that protects the interior of our body from a variety of external environments and is the outermost organ of our body. These skins contain keratinocytes, which inhibit the invasion of foreign substances, Langerhans cells and macrophages, which are responsible for skin immunity, fibroblasts, which maintain skin tissue, and pigment production. There are melanocytes (melanocytes) and the like.

Melanin is a phenolic polymer natural pigment widely present in living organisms. In the human body, melanin is synthesized in melanosomes in melanocytes in the basal epidermal layer and transferred to surrounding keratinocytes to represent human skin color. Abnormally low melanin causes skin lesions, such as vitiligo, and, conversely, if overproduced, acquired hyperpigmentation such as melanoma, postinflammatory melanoderma, and solar lentigo. It can cause a variety of skin diseases, including.

The synthesis of melanin is made by making melanocytes from melanocytes (melanocytes) in the base layer of the skin and collectively referred to as melanogenesis. The production of melanin is based on tyrosine, one of the amino acids, and DOPA (3,4-) by tyrosinase, tyrosinase related protein-1 (TRP-1) and tyrosinase related protein-2 (TRP-2). After conversion to dopa quinone via dihydroxyphenylalanine, melanin is synthesized by non-enzymatic reaction, spontaneous oxidation and polymerization with amino acid or protein (Korean J. FOOD SCI. TECHNOL., 2000. 32 (3): 736; Journal of Life Science, 2013. 23 (12): 1445; Pigment Cell Res. 1999. 12 (1): 4-12.). Recently, studies on inhibition of melanin synthesis have been made using proteins such as microphtalmia associated transcription factor (MITF) as well as tyrosinase, TRP-1, and TRP-2. The major intracellular signaling pathway is the cAMP / PKA (cyclic monophosphate / protein kinase A) pathway. CAMP promotes the expression of MITF via PKA and CREB1 (cAMP responsive element binding protein 1), and MITF is important for melanin synthesis. Promote transcription of tyrosinase, TRP-1, TRP-2 as a transcriptional regulator (Pharmazie. 2015 Oct; 70 (10): 646-9 .; Int J Mol Sci. 2015 Oct 13; 16 (10): 24219- 42; Curr Pharm Biotechnol. 2015; 16 (12): 1111-9.).

Because tyrosinase plays the most important role as the first step in producing melanin, the study of whitening agents is mainly used to inhibit tyrosinase activity or antagonists of tyrosinase substrates in order to regulate tyrosinase activity. Research has been underway towards the development of (Kor. J. Pharmacogn., 44 (3): 220, 2013).

Arbutin, the most commonly used whitening agent, is an antagonist of tyrosine, and kojic acid inhibits the process of tyrosine to dopa and dopaquinone by chelating tyrosinase active sites (Korean J. Plant Res., 26 (5): 526, 2013; J. Korean Soc.Food Sci.Nutr., 41 (2): 156, 2012). Arbutin and kojic acid are known to have a strong whitening effect, but due to some side effects such as skin irritation, research has been actively conducted to find a material having a whitening activity from a relatively low side effects of natural substances.

The present invention discloses tyrosinase inhibitory activity and melanogenesis inhibitory activity of ginsenoside compound K and rice bran extract.

An object of the present invention to provide a composition for skin whitening using ginsenoside compound K and rice bran extract.

Other and specific objects of the present invention will be presented below.

The present invention is completed by confirming that ginsenoside compound K, rice bran extract and mixtures thereof all have tyrosinase inhibitory activity and melanin production inhibitory activity, as confirmed in the following Examples and Experimental Examples.

Considering the above, the skin whitening composition of the present invention is characterized in that it comprises ginsenoside compound K, rice bran extract and mixtures thereof as an active ingredient.

In the present invention, the ginsenoside compound K (20-O-β-D-glucopyranosyl-20 (S) -protopanaxadiol) is a saponin produced by decomposition of Rb1, Rb2, and Rc, PPD-based saponins, by human intestinal bacteria Say In general, many glycoside compounds present in nature tend to increase physiological activity when sugars are broken down into non-saccharides rather than by themselves (Med. Pharm. Soc. 1992, 9: 1-13). In the case of ginseng saponins, ginsenosides Rg3, Rh1, Rh2, F2, compound Y, compound K, etc., formed by hydrolysis of some of the sugars than ginsenosides Rb1, Rb2, Rd, and Re, in which three or more sugars are bonded. It is known to have a much superior effect in terms of absorption into the living body and physiological activity (Ginseng Res. 2003, 27: 129-134).

In addition, in the present invention, the rice bran extract is water to be extracted rice bran, lower alcohol (methanol, ethanol, butanol, etc.) of 1 to 4 carbon atoms, methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N Supercritical extraction solvents such as carbon dioxide, pentane, extracts obtained by leaching with N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or a mixed solvent thereof Means an extract obtained by fractionating the extract or the extract thereof, and the extraction method may be applied to any method such as cooling, refluxing, heating, ultrasonic radiation, and supercritical extraction in consideration of the polarity, the degree of extraction, and the degree of preservation of the active substance. Can be. In the case of the fractionated extract, the fraction obtained by suspending the extract in a specific solvent and mixing and standing with a solvent having a different polarity, the crude extract is adsorbed on a column filled with silica gel and the like, and then a hydrophobic solvent, a hydrophilic solvent, or a mixed solvent thereof is added. It is meant to include fractions obtained by mobile phase. In addition, the meaning of the extract includes a concentrated liquid extract or solid extract in which the extraction solvent is removed in a manner such as freeze drying, vacuum drying, hot air drying, spray drying, and the like. Preferably it refers to an extract obtained by using water, ethanol or a mixed solvent thereof as the extraction solvent, more preferably an extract obtained by using a mixed solvent of water and ethanol as the extraction solvent.

In addition, the active ingredient in the present invention means a component that can exhibit the desired activity alone or in combination with a carrier having no activity.

In the composition for skin whitening of the present invention, the active ingredient may be included in any amount (effective amount) depending on the dosage form, etc., as long as it can exhibit skin whitening activity, and a typical effective amount is 0.001 weight based on the total weight of the composition. It will be determined in the range of% to 20.0 weight%. The term "effective amount" herein refers to the intended functional and pharmacological effects of skin whitening effect, such as when the composition of the present invention is administered or used to a mammal, preferably a human, for the administration or use of a suggestion by a medical expert, a skin expert, or the like. It refers to the amount of the active ingredient included in the composition of the present invention, which can exhibit the effect. Such effective amounts can be determined experimentally within the range of ordinary skill in the art.

The skin whitening composition of the present invention, in addition to the active ingredient, enhances or enhances the skin whitening activity or enhances the convenience of taking or using the skin by adding skin-related activities such as an anti-wrinkle improvement component, a UV protection component, and a skin moisturizing component. To this end, it may further comprise any compound or natural extract already known in the art that has been tested for safety and has the corresponding activity. These compounds or extracts include pharmacopoeia of each country ("Korea Pharmacopoeia" in Korea), health functional food code of Korea (in Korea, "health and functional food standards and standards" noticed by Korea Food and Drug Administration), and functional cosmetics public notice (KFDA notice in Korea " Compounds or extracts contained in the process documents, such as functional cosmetic standards and test methods "), compounds or extracts which have been approved under the laws of each country (" Pharmaceutical Affairs Law "in Korea) that govern the manufacture and sale of medicines, health functions Includes compounds or extracts whose functionality has been recognized in accordance with national legislation governing the manufacture and sale of foods (in Korea, the Act on Health Functional Foods).

Specifically, skin whitening ingredients include arbutin, niacinamide, ascorbyl glucoside, alpha-bisabolol, oil soluble licorice (Glycyrrhiza) extract, and the like as retinol and retinyl palmi. Tate, adenosine, a polyethoxylated amide, etc., As a ultraviolet protection component, dromethazole, a dromethazole trisiloxane, the digaloyl tri oleate, the dimethco diethyl benzal malonate, the diehexyl buty Butami Complexes such as dotriazone, pine bark extract, phosphatidylserine, finger root extract powder, complex extracts such as red ginseng and cornus milk. The moisturizing component includes AP collagen degrading peptide, Collactive collagen peptide, N-acetylglucosamine, konjac potato extract, dandelion and other complex extracts, rice bran extract, corn germ extract, low molecular collagen peptide, herbal extract powder, phosphatidylserine, hyaluronic acid, etc. Can be mentioned. Such ingredients may be included in the composition for skin whitening of the present invention together with one or more of its active ingredients.

The skin whitening composition of this invention can be grasped | ascertained as a cosmetic composition in a specific aspect.

Even when the composition of the present invention is identified as a cosmetic composition, the cosmetic composition can be classified into any product according to its purpose and law, and specifically, a functional cosmetic having a use such as skin trouble improvement, atopic dermatitis improvement, and non-functionality. General cosmetics, and the like. The product form can also be in any product form, specifically solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, waxes You can find product types such as foundation and spray. In a specific product form, it may be a softening lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder formulation.

The cosmetic composition of the present invention may include, in addition to the active ingredient, components conventionally used in the cosmetic composition, for example, conventional adjuvants such as stabilizers, solubilizers, surfactants, vitamins, pigments and pharmaceuticals, and carriers.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as the carrier component, specifically water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan and the like can be used.

When the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, and microcrystals are used as carrier components. Castle cellulose, aluminum metahydroxy, bentonite, agar and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention can be prepared according to the manufacturing method of the cosmetic composition usually carried out in the art, except for including the active ingredient exhibiting skin whitening activity.

The skin whitening composition of this invention can be grasped | ascertained as a food composition in another specific aspect.

The food composition of the present invention may be prepared in any form, for example, beverages such as tea, juice, carbonated beverages, ionic beverages, processed oils such as milk, yogurt, gums, rice cakes, sweets, breads, Foodstuffs such as confectionery, cotton, tablets, capsules, pills, granules, liquids, powders, flakes, pastes, syrups, gels, jelly, bars, and health functional food preparations such as bars.

In addition, the food composition of the present invention can distinguish any product as long as it conforms to the enforcement regulations at the time of manufacture and distribution in the legal and functional divisions. For example, confectionery, soybeans, teas, beverages according to each food type, or health functional foods in accordance with the Act on Health Functional Foods, or in the Food Code of the Korean Food Sanitation Act , Special purpose foods, and the like.

The food composition of the present invention may include food additives in addition to the active ingredient. Food additives can generally be understood as substances which are added to the food, mixed or infiltrated in the manufacture, processing or preservation of the food, which must be ensured because of its daily and long-term intake with the food. The Food Additives Code of Korea ("Food Sanitation Act" in Korea) governs the manufacture and distribution of foods, and food additives with guaranteed safety are limited in terms of ingredients or functions. In the Korean Food Additives Code (KFDA Notification and Standards of Food Additives), food additives are classified into chemical synthetics, natural additives, and mixed preparations in terms of ingredients. These food additives are sweeteners, flavors in terms of function. Agent, preservative, emulsifier, acidulant, thickener and the like.

Sweeteners are used to impart a proper sweetness to foods, and may be natural or synthesized. Preferably, a natural sweetener is used. Examples of the natural sweetener include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavoring agents can be used to enhance the taste or aroma, both natural and synthetic. It is the case of using a natural thing preferably. In addition to flavors, the use of natural ones can be combined with nutritional purposes. The natural flavor may be obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or may be obtained from green tea leaves, round leaves, jujube leaves, cinnamon, chrysanthemum leaves, jasmine and the like. In addition, ginseng (red ginseng), bamboo shoots, aloe vera, ginkgo and the like can be used. Natural flavors can be liquid concentrates or solid extracts. In some cases, a synthetic flavor may be used, and as the synthetic flavor, esters, alcohols, aldehydes, terpenes, and the like may be used.

As the preservative, calcium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid) and the like can be used, and as an emulsifier, acacia gum, carboxymethylcellulose, xanthan gum, pectin Etc. may be used, and acidic acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, and the like may be used. The acidulant may be added so that the food composition is at an appropriate acidity for the purpose of inhibiting the growth of microorganisms in addition to the purpose of enhancing the taste.

As the thickener, suspending implementers, sedimenting agents, gel formers, swelling agents and the like can be used.

In addition to the food additives described above, the food composition of the present invention may include a bioactive substance or minerals known in the art for the purpose of supplementing and reinforcing the functionality and nutritional properties and ensuring the stability as a food additive.

Examples of such physiologically active substances include catechins, vitamin B1, vitamin C, vitamin E, vitamin B12, tocopherol, dibenzoyl thiamine, and the like contained in green tea. Examples of the minerals include calcium preparations such as calcium citrate and magnesium stearate. Magnesium preparations, iron preparations such as iron citrate, chromium chloride, potassium iodine, selenium, germanium, vanadium, zinc and the like.

In the food composition of the present invention, the food additive as described above may be included in an amount that can achieve the purpose of addition according to the product type.

Regarding other food additives that may be included in the food composition of the present invention, reference may be made to each country's food or food additives.

The composition for skin whitening of the present invention can be regarded as a pharmaceutical composition in specific embodiments.

When the composition for skin whitening of the present invention is identified as a pharmaceutical composition, the target disease may be understood as skin hyperpigmentation, and specific examples of such hyperpigmentation include freckles, senile spots, liver spots, blemishes, brown or sunspots, and sun pigments. Half, cyanic melasma, hyperpigmentation after use of drugs such as calcium antagonists, sequelae following sclerotherapy, gravidic chloasma, melanoma in women taking oral contraceptives, Post-inflammatory hyperpigmentation, phototoxic reactions or other similar small fixed pigmented lesions, including, but not limited to, wounds or dermatitis, including scratches and burns.

The pharmaceutical compositions of the present invention may be prepared in oral or parenteral formulations according to the route of administration by conventional methods known in the art, including pharmaceutically acceptable carriers in addition to the active ingredient. The route of administration here can be any suitable route, including the topical route, the oral route, the intravenous route, the intramuscular route, and direct absorption through mucosal tissue, and may be used in combination of two or more routes. An example of a combination of two or more routes is where a drug of two or more formulations according to the route of administration is combined, for example when one drug is administered first by the intravenous route and the other by the local route.

Pharmaceutically acceptable carriers are well known in the art depending on the route of administration or formulation, and specific reference may be made to the pharmacopoeia of each country, including "Korea Pharmacopoeia."

When the pharmaceutical composition of the present invention is prepared in an oral dosage form, powders, granules, tablets, pills, dragees, capsules, solutions, gels, syrups, suspensions, wafers according to methods known in the art with suitable carriers It may be prepared in a formulation such as. Examples of suitable carriers include lactose, glucose, sucrose, dextrose, sorbitol, sugars such as mannitol, xylitol, starch such as corn starch, potato starch, wheat starch, cellulose, methyl cellulose, ethyl cellulose, sodium carboxymethyl cellulose, Celluloses such as hydroxypropylmethylcellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol, vegetable oil, ethanol, and Cerrol etc. are mentioned. If formulated, appropriate binders, lubricants, disintegrants, colorants, diluents and the like may be included as needed. Suitable binders include starch, magnesium aluminum silicate, starch ferrite, gelatin, methylcellulose, sodium carboxymethylcellulose, polyvinylpyrrolidone, glucose, corn sweeteners, sodium alginate, polyethylene glycol, waxes, and the like. Sodium, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, silica, talcum, stearic acid, magnesium salts thereof, calcium salts, polydetylene glycol and the like, and the disintegrating agents include starch, methyl cellulose And agar, bentonite, xanthan gum, starch, alginic acid or its sodium salt and the like. Moreover, lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, glycine etc. are mentioned as a diluent.

When the pharmaceutical compositions of the present invention are prepared in parenteral formulations, they may be formulated in the form of injections, transdermal administrations, nasal inhalants and suppositories with suitable carriers according to methods known in the art. When formulated as an injection, a suitable carrier may be an aqueous isotonic solution or suspension. Specifically, an isotonic solution such as phosphate buffered saline (PBS) containing triethanol amine, sterile water for injection, or 5% dextrose may be used. . When formulated as a transdermal administration, it may be formulated in the form of an ointment, cream, lotion, gel, external solution, pasta, linen, aerosol, and the like. Nasal inhalants can be formulated in the form of aerosol sprays using suitable propellants, such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide, etc. witepsol), tween 61, polyethylene glycols, cacao butter, laurin paper, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearate, sorbitan fatty acid esters and the like.

Specific formulations of pharmaceutical compositions are known in the art and can be found, for example, in Remington's Pharmaceutical Sciences (19th ed., 1995). The document is considered part of this specification.

Preferred dosages of the pharmaceutical compositions of the present invention range from 0.001 mg / kg to 10 g / kg per day, preferably 0.001 mg / kg to 1 g, depending on the condition, body weight, sex, age, severity of the patient and route of administration. It can range from / kg. Administration can be done once a day or divided into several times. Such dosage should not be construed as limiting the scope of the invention in any aspect.

As described above, according to the present invention can provide a composition for skin whitening using ginsenoside compound K and rice bran extract.

The composition for skin whitening of the present invention may be commercialized into cosmetics, foods, drugs and the like.

1 shows the results of tyrosinase inhibitory activity of ginsenoside compound K and rice bran extract.
Figure 2 shows the results of tyrosinase inhibitory activity of ginsenoside compound K and rice bran extract mixture.
Figure 3 is a result of the melanin production inhibition experiments of ginsenoside compound K and rice bran extract and mixtures thereof.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Skin Whitening Activity of Ginsenoside Compound K and Rice Bran Extract

1. Preparation of Sample

CK used a 30% pure self-produced and purified, rice bran extract was added to 10 times the weight 80% ethanol to dry rice bran powder, extracted at room temperature for 24 hours, and then filtered to remove the extraction residue, the filtrate Concentrated under reduced pressure and lyophilized to prepare a powder to be used in the experiment.

2. Experiment Method

2-1. Tyrosinase Inhibitory Activity Experiment

Dispense 1 mL of 0.1M sodium phosphate solution (pH 6.5) into a 24-well microplate, add 0.2 mL of 100 μg / mL sample solution, and add 0.2 mL of 2,000 U / mL tyrosinase and 0.2 mL of 7.5 mM tyrosine. The reaction was carried out at 37 ° C. for 20 minutes. After the reaction, the microplate with the reaction solution was quickly transferred to ice, quenched to stop the reaction, and the absorbance was measured at 490 nm using a microplate reader. The results were calculated as a percentage of tyrosinase inhibitory activity (%) compared to the sample untreated group. Arbutin was used as a positive control.

2-2. Melanin production inhibition experiment

Mouse melanoma cell line B16F10 cells were purchased from Korea Cell Line Bank (Seoul, Korea) and cultured in DMEM medium containing 10% FBS and 1% penicillin / streptomycin at 37 ° C. and 5% CO ₂ .

B16F10 cells were aliquoted at 2.5 × 10 ⁵ cells / mL in a 24-well plate and pre-incubated for 18 hours using DMEM medium supplemented with 10% FBS. Samples prepared by nM and concentration were co-treated and incubated in a 37%, 5% CO ₂ cell incubator for 48 hours. After 48 hours, the medium of the plate was removed and washed twice with PBS. Next, the cells were washed with phosphate buffer (pH 7.4), and cell lysis buffer (100 mM sodium phosphate (pH6.8), 0.1 mM PMSF, 1% Triton X-100) was added, and the cells were lysed at -80 ° C for 30 minutes. The lysed cells were harvested, 1 N NaOH was added to completely dissolve the cells, and the absorbance was measured by ELISA at 405 nm. The results were obtained as a percentage of the treated group of α-MSH alone.

3. Experimental Results

3-1. Tyrosinase Inhibitory Activity Test Results

The tyrosinase inhibitory activity test results are shown in FIG. 1 below.

Referring to Figure 1 it can be seen that the CK and rice bran extract, the mixture tyrosinase activity inhibited, the inhibitory effect of tyrosinase activity of the CK and rice bran extract mixture is 5: 5 (CK: rice bran extract) to 2 It was especially high in the mixing ratio (weight ratio) of: 8 (CK: rice bran extract) (A: CK alone, B: CK and rice bran extract = 8: 2, C: CK and rice bran extract = 6: 4, D: CK and Rice bran extract = 5: 5, E: CK and rice bran extract = 4: 6, F: CK and rice bran extract = 2: 8, G: rice bran extract alone).

Figure 2 shows the tyrosinase inhibitory activity according to the treatment concentration of the mixture of the CK and rice bran extract of the most excellent activity, 4: 6 weight ratio. 2 shows by inhibiting tyrosinase activity in proportion to treatment concentration.

3-2. Melanin production inhibition test result

Melanin production inhibition test results are shown in FIG. 3.

Referring to Figure 3 shows that the melanin production inhibitory effect of the mixture of the mixture (E) of 4: 6 weight ratio of CK and rice bran extract than the effect of inhibiting the melanin production of CK (A) or rice bran (G), The mixture is shown to inhibit melanin production in a concentration dependent manner.

Claims (5)

Contains a mixture of ginsenoside compound K and rice bran extract as an active ingredient,
The mixture is a ginsenoside compound K and rice bran extract is a mixture of 5: 5 to 2: 8 weight ratio, the composition for skin whitening.
The method of claim 1,
The rice bran extract is water, ethanol or a mixture for skin whitening, characterized in that the mixed solvent extract.
The method according to claim 1 or 2,
The composition for skin whitening, characterized in that the cosmetic composition.
The method according to claim 1 or 2,
The composition is a composition for skin whitening, characterized in that the food composition.
The method according to claim 1 or 2,
The composition for skin whitening, characterized in that the pharmaceutical composition.

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