KR102104850B1 - Skin-lightening Composition Using an Extract of Polygonum amphibium - Google Patents

Abstract

The present invention discloses a composition for skin whitening using water extract having a melanin synthesis inhibitory ability.

Description

Skin-lightening composition using an extract of polygonum amphibium

The present invention relates to a composition for skin whitening using an extract of Polygonum amphibium .

As the standard of living in modern society improves, interest in beauty as well as health is gradually increasing. Among them, it is actively developing products for improving pigmentation-type skin diseases such as melasma and freckle caused by skin damage and aging caused by ultraviolet rays and air pollution, and improving overall skin tone. Melanin, which is present in the base layer of the epidermis, is a major pigment that determines skin color. It prevents damage by ultraviolet rays and removes free radicals to protect the skin. However, overexpression of melanin is greatly affected not only in skin health such as skin darkening, pigmentation, blemishes, freckles, skin cancer, etc. (Pigment Cell Res 10, 127-138. 1997; Vet Dermatol. 2003 Apr; 14 (2) : 57-65.).

It is known that melanin is synthesized from melanocytes, which are color cells present in the base layer in the skin (epidermis), and is metastasized to peripheral keratinocytes, and is known to exhibit human skin color. When the skin receives sunlight, melanin is produced by chemical reactions in melanocytes, pigment-forming cells. The main role of melanin is to protect the inside of the skin by removing free radicals and free radicals generated by the skin (Brain Research Bulletin 10 (6): 847-851, 1983; Mutation) Research 571 (1-2): 121-32, 2005; Nature. 22; 445 (7130): 843-50. 2007).

The starting material for melanin is tyrosine, a type of amino acid normally present in the human body. Tyrosine is oxidized in the melanocytes by an enzyme called tyrosinase to turn into dopa (dihydroxy phenylalanine, DOPA), and dopa further oxidizes to produce dopaquinone (DOPA-Quinone). And when dopaquinone encounters cysteine, cysteinyldopa is produced, resulting in a reddish-brown pheomelanin. The produced melanin is then carried on a melanosome and delivered to keratinocytes (Pigment Cell Res. 12 (1): 4-12.1999 .; Cell Mol Biol (Noisy-le-grand). 45 (7): 981 -90.1999; An Bras Dermatol. 88 (1): 76-83. 2013).

The basic mechanisms of drugs formulated in whitening cosmetics for the purpose of preventing or alleviating pigmentation are inhibiting tyrosinase action, inhibiting tyrosinase production, inhibiting melanin production mediators, inhibiting melanin reduction and strong oxidation, promoting melanin excretion, And sun protection. Previous studies on whitening have often screened the degree of tyrosinase activity, which plays a major role in the process of melanin synthesis, to develop whitening materials, but recently, at various stages such as melanin production, migration, and scale. By controlling various mechanisms, there are increasing cases in which various mechanisms are studied to develop whitening materials with better efficacy (Lee et al., J Soc Cosmet Scientists Korea 37, 275-822. 2011). Antioxidants such as vitamin C, glutathione, and ascorbic acid phosphate ester magnesium are known as whitening agents that reduce the produced melanin (J Cosmet Sci. 65 (6): 365-75. 2014; Int J Cosmet Sci. 37 (6): 567-73. 2015; Yakugaku Zasshi. 128 (8): 1203-7.2008; Int J Cosmet Sci.27 (3): 147-53. 2005; Acta Derm Venereol.63 (3): 209 -14.1983; Skin Pharmacol Appl Skin Physiol. 13 (3-4): 143-9.2000; J Cosmet Laser Ther.15 (2): 107-13. 2013.).

Since various kinds of related factors are involved in the biosynthesis of melanin, various experimental methods are applied in addition to the simple melanin synthesis inhibition experiment. Recently, research using zebrafish has been actively conducted. Since zebrafish can obtain more than 100 eggs at a time, it is possible to secure a large amount of samples, and it is possible to immediately observe whether pigments are generated through transparent skin (Grunwald and Eisen, Nat Rev) Genet, 3: 717-724, 2002).

As interest in whitening has increased, whitening cosmetics with more excellent effects have been actively developed in addition to the existing well-known substances, but synthetic whitening materials have problems such as decomposition, coloring, odor, stability and safety during use. . Therefore, studies on natural whitening agents that do not affect cells and reduce melanin synthesis are being actively conducted.

An object of the present invention is to provide a composition for skin whitening using water extract.

Other or specific objects of the present invention will be presented below.

The present invention was completed by confirming that the extract is water-extracted and has a melanin synthesis inhibitory ability in mouse-derived melanoma cell line B16F10 and also has melanin biosynthesis inhibitory activity in zebrafish, as shown in the following examples and experimental examples.

Considering the above, the composition for skin whitening of the present invention is characterized by including water extract as an active ingredient.

In the present specification, the term "water quenched extract" refers to water quenched outpost, leaves, stems, ground, roots, roots, underground parts or mixtures thereof, water, and lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.), Methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N, N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or their Extracts obtained by leaching using mixed solvents, extracts obtained using supercritical extraction solvents such as carbon dioxide and pentane, or fractions obtained by fractionating the extracts. The extraction method considers the polarity, extraction degree, and storage degree of the active substance. Thus, any method such as cold immersion, reflux, warming, ultrasonic radiation, and supercritical extraction can be applied. In the case of fractionated extracts, the fractions obtained by suspending the extracts in a specific solvent and mixing and policing with a solvent of different polarity, adsorb the crude extract on a column filled with silica gel, etc., and then use a hydrophobic solvent, a hydrophilic solvent or a mixed solvent thereof. It means that it contains the fraction obtained as a mobile phase. In addition, the meaning of the extract includes a concentrated liquid extract or a solid extract in which the extraction solvent is removed by a method such as freeze drying, vacuum drying, hot air drying, spray drying, and the like. Preferably, it means an extract obtained by using water, ethanol or a mixed solvent thereof as an extraction solvent, in particular an extract obtained by using ethanol as an extraction solvent. More preferably, the ethanol extract of ethanol acetate-soluble extract, more preferably, after ethanol extract is suspended in water, the extract according to each fraction obtained by sequentially fractionation using normal hexane, dichloromethane, ethyl acetate and normal butanol, In particular, it means a fraction of the ethyl acetate layer. Here, "sequential fractionation" means that the fraction of the water layer is continuously used after fractionation and fractionated with a solvent in the order listed above.

In addition, in the present specification, "active ingredient" refers to a component that can exhibit the desired activity alone or itself can exhibit activity with an inactive carrier.

The composition of the present invention may contain the active ingredient in any amount (effective amount) as long as it can exhibit skin whitening activity, etc. according to the use, formulation, blending purpose, etc., the typical effective amount is based on the total weight of the composition It will be determined within the range of 0.001% to 15% by weight. Here, the "effective amount" refers to the intended medical and dermatological effect, such as skin whitening effect, when the composition of the present invention is administered to a mammal, preferably a person, to which a target is applied, by a medical expert, a skin expert, etc. It refers to the amount of active ingredients contained in the composition of the present invention. Such an effective amount can be determined empirically within the ordinary skill in the art.

The composition for skin whitening of the present invention, in addition to the active ingredient, may further include any compound or natural extract known in the art as having safety and verified safety in the art for enhancing and adding skin-related activity. You can.

Specifically, examples of skin whitening ingredients include arbutin, niacinamide, ascorbyl glucoside, alpha-bisabolol, oil soluble licorice (Glycyrrhiza) extract, and retinol, retinyl palmimi as skin wrinkle improvement ingredients. Tate, adenosine, polyethoxylated amide, and the like, and examples of UV protection components include drometrizole, drometrizoletrisiloxane, digalloyl trioleate, dimethycodiethyl benzalmalonate, and diethylhexylbutami And complexes such as dotriazone and pine bark extract, phosphatidylserine, fingerroot extract powder, and complex extracts such as red ginseng and cornus officinalis. In addition, as a moisturizing ingredient, AP collagen enzyme degrading peptide, Collactive collagen peptide, N-acetylglucosamine, konjac potato extract, dandelion, etc., complex extracts, rice bran extract, corn germ extract, low molecular collagen peptide, ground extract powder, phosphatidylserine, hyaluronic acid, etc. Can be mentioned. For other skin whitening ingredients, wrinkle improvement ingredients, UV protection ingredients, and skin moisturizing ingredients, functional cosmetics exhibitions in each country (in Korea, it is the "standard and test method for functional cosmetics" announced by the Ministry of Food and Drug Safety), health functional foods exhibition in each country (in Korea The Ministry of Food and Drug Safety may refer to "Health functional food standards and standards." These ingredients may be included in the composition for skin whitening of the present invention together with one or more of its active ingredients.

In the composition for skin whitening of the present invention, the active ingredient may be included in any amount (effective amount) according to use, formulation, blending purpose, etc., as long as it can exhibit skin whitening activity, and the typical effective amount is based on the total weight of the composition It will be determined within the range of 0.001% by weight to 20.0% by weight. Here, "effective amount" refers to the amount of active ingredients that can induce skin whitening effects. Such an effective amount can be determined empirically within the ordinary skill in the art.

In the specific aspect, the composition for skin whitening of the present invention can be identified as a cosmetic composition.

Even if the composition of the present invention is identified as a cosmetic composition, the cosmetic composition may be classified into any product in accordance with its use, and may be a functional cosmetic, non-functional general cosmetic, or the like specifically having a purpose such as skin whitening. . As for the product form, any product form can be taken, specifically, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, waxes It can be formulated as a foundation, spray, or the like. In a specific product form, it may be flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder formulation.

The cosmetic composition of the present invention may include, in addition to its active ingredients, ingredients commonly used in cosmetic compositions, such as stabilizers, solubilizers, surfactants, vitamins, pigments and conventional auxiliary agents such as colorants and carriers.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. may be used as a carrier component. You can.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be used as a carrier component, and in the case of a spray, additionally chlorofluorohydrocarbon, propane / Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, and the like can be used.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene sorbitol esters, polyoxyethylene sorbitan esters, and microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide as a carrier component Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention can be prepared according to the manufacturing method of a cosmetic composition that is conventionally performed in the art, except that it contains an active ingredient that exhibits skin whitening activity.

In the specific aspect, the composition of the present invention can be grasped as a food composition. When the composition of the present invention is identified as a food composition, its use may be understood as inhibiting skin hypersensitivity or inhibiting hypersensitivity reactions.

The food composition of the present invention can be produced in any form, such as tea, juice, carbonated beverages, beverages such as ionic beverages, processed oils such as milk, yogurt, gums, rice cakes, Chinese medicines, breads, cookies, noodles, etc. Food, tablets, capsules, pills, granules, liquids, powders, flakes, pastes, syrups, gels, jelly, bars, etc. can be prepared as health functional food formulations.

In addition, the food composition of the present invention can be classified into any product as long as it complies with the enforcement regulations at the time of manufacturing and distribution in the legal and functional classification. For example, it is a health functional food pursuant to the Korean Act on Health Functional Food, or a confectionary, soybean, tea, or beverage according to each food type in the Food Fair of the Korean Food Sanitation Act (「Food Standards and Standards」) , Special purpose foods, and the like.

Food composition of the present invention may include a food additive in addition to the active ingredient. Food additives can be generally understood as substances added to foods, mixed or infiltrated in the manufacture, processing, or preservation of foods, and their safety must be ensured because they are consumed daily and for a long time with foods. Food additives in accordance with national laws governing the manufacture and distribution of food ("Food Sanitation Act" in Korea) are limited in terms of ingredients or functions in terms of safety and food additives. In the Korean Food Additives Fair (the Ministry of Food and Drug Safety's 「Food Additive Standards and Standards」), food additives are divided into chemical synthetic products, natural additives, and mixed preparations in terms of ingredients. It is divided into agents, preservatives, emulsifiers, acidulants, and thickeners.

Sweeteners are used to impart a moderate sweetness to food, and both natural and synthetic can be used in the composition of the present invention. Preferably, a natural sweetener is used, and examples of the natural sweetener include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose, and maltose.

Flavoring agents can be used to enhance the taste or aroma, and both natural and synthetic can be used. Preferably, it is the case of using a natural thing. In addition to flavor, when using natural ones, the purpose of enhancing nutrition can also be combined. As a natural flavoring agent, it may be obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, etc., or may be obtained from green tea leaves, perilla, large leaves, cinnamon, chrysanthemum leaves, jasmine, and the like. In addition, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, and ginkgo can be used. Natural flavoring agents may be liquid concentrates or solid extracts. In some cases, synthetic flavoring agents may be used, and synthetic flavoring agents may include esters, alcohols, aldehydes, terpenes, and the like.

As a preservative, sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid) and the like can be used, and as an emulsifier, acacia gum, carboxymethylcellulose, xanthan gum, Pectin and the like, and as the acidulant, arithmetic, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid and the like can be used. The acidulant may be added so that the food composition has an appropriate acidity for the purpose of suppressing the growth of microorganisms in addition to the purpose of enhancing taste.

As a thickener, a suspending agent, sedimentation agent, gel forming agent, swelling agent, etc. may be used.

The food composition of the present invention may include bioactive substances or minerals known in the art for the purpose of supplementing and reinforcing functional and nutritional properties in addition to the food additives described above, and guaranteed stability as food additives.

Examples of such bioactive substances include catechins contained in green tea, vitamins such as vitamin B1, vitamin C, vitamin E, and vitamin B12, tocopherol, dibenzoyl thiamine, and the like, and calcium preparations such as calcium citrate and magnesium stearate as minerals Magnesium preparations such as iron, iron preparations such as iron citrate, chromium chloride, potassium iodide, selenium, germanium, vanadium, zinc, and the like.

The food composition of the present invention may include the food additives as described above in an appropriate amount to achieve the purpose of addition according to the product type.

With regard to other food additives that may be included in the food composition of the present invention, it is possible to refer to national foods or food additives.

The composition for skin whitening of the present invention can be identified as a pharmaceutical composition in a specific embodiment.

When the composition for skin whitening of the present invention is identified as a pharmaceutical composition, the target disease may be understood as skin hyperpigmentation, and specific examples of such hyperpigmentation are freckles, senile plaques, liver spots, spots, brown or black spots, and sun pigment Wounds or dermatitis, including hyperpigmentation after use of drugs such as half, blue dermatosis, calcium antagonists, hyperpigmentation sequelae according to tissue sclerotherapy, gestational brown spots, black dermatosis, scratches and burns in women taking oral contraceptives After inflammation caused by hyperpigmentation, phototoxic reactions or other similar small fixed pigmented lesions, but may be included, but is not limited thereto.

The pharmaceutical composition of the present invention may be prepared in an oral dosage form or a parenteral dosage form according to the route of administration by a conventional method known in the art, including a pharmaceutically acceptable carrier in addition to the active ingredient. Here, the route of administration may be any suitable route including a local route, an oral route, an intravenous route, an intramuscular route, and direct absorption through mucosal tissue, and two or more routes may be used in combination. An example of a combination of two or more routes is when two or more formulations of the drug are combined according to the route of administration, for example, when one drug is administered by intravenous route and the second drug is administered by topical route.

Pharmaceutically acceptable carriers are well known in the art depending on the route of administration or formulation, and specifically refer to the pharmacopeia of each country, including "Korea Pharmacopoeia".

When the pharmaceutical composition of the present invention is prepared in an oral dosage form, powders, granules, tablets, pills, dragees, capsules, liquids, gels, syrups, suspensions, wafers according to methods known in the art with suitable carriers And the like. Examples of suitable carriers at this time include sugars such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol, xylitol, starches such as corn starch, potato starch, wheat starch, cellulose, methylcellulose, ethylcellulose, sodium carboxymethylcellulose, Celluloses such as hydroxypropyl methylcellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol, vegetable oil, ethanol, green Serol, etc. are mentioned. In the case of formulation, if necessary, suitable binders, lubricants, disintegrants, colorants, diluents, etc. may be included. Suitable binders include starch, magnesium aluminum silicate, starch ferist, gelatin, methylcellulose, sodium carboxymethylcellulose, polyvinylpyrrolidone, glucose, corn sweetener, sodium alginate, polyethylene glycol, wax, etc., and lubricants include oleic acid Sodium, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, silica, talcum, stearic acid, magnesium salts and calcium salts thereof, polydetylene glycol, and the like, and starch and methyl cellulose as disintegrants , Agar, bentonite, xanthan gum, starch, alginic acid or its sodium salt. Moreover, lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, glycine etc. are mentioned as a diluent.

When the pharmaceutical composition of the present invention is prepared in a parenteral dosage form, it may be formulated in the form of injections, transdermal administrations, nasal inhalants and suppositories according to methods known in the art with suitable carriers. When formulated as an injectable agent, an aqueous isotonic solution or suspension may be used as a suitable carrier. Specifically, an isotonic solution such as PBS (phosphate buffered saline) containing triethanol amine, sterile water for injection, or 5% dextrose may be used. . When formulated as a transdermal dosage form, it can be formulated in the form of ointments, creams, lotions, gels, external solutions, pasta, linen agents, aerosols, and the like. For nasal inhalers, dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide, etc. can be formulated in the form of an aerosol spray using a suitable propellant. witepsol), tween 61, polyethylene glycols, cacao butter, laurin, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearate, sorbitan fatty acid esters, and the like.

Regarding the specific formulation of the pharmaceutical composition, it is known in the art, and for example, see Remington's Pharmaceutical Sciences (19th ed., 1995) and the like. The above documents are regarded as part of this specification.

Preferred dosages of the pharmaceutical compositions of the present invention range from 0.001 mg / kg to 10 g / kg per day, preferably 0.001 mg / kg to 1 g per day, depending on the patient's condition, weight, sex, age, patient severity, and route of administration. / kg range. Administration can be made once a day or divided into several times. Such dosages should not be construed as limiting the scope of the invention in any aspect.

As described above, according to the present invention, it is possible to provide a composition for skin whitening using water extract.

The composition for skin whitening of the present invention can be commercialized as food, cosmetics, and drugs.

1 is a schematic diagram showing the manufacturing process of the water extract and its fraction.
2 to 4 is a result showing the melanin synthesis inhibitory ability in the mouse-derived melanoma cell line B16F10 of water extract and its fraction.
5 is a result showing the cytotoxicity in the mouse-derived melanoma cell line B16F10 of the ethyl acetate fraction.
6 is a result showing the ability to inhibit melanin synthesis in zebrafish of ethyl acetate fraction.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Water extract of  Produce

20% by weight of 100% ethanol (HPLC grade) was added to the water filtration (outpost) powder, leached for 24 hours, filtered with Whatman paper filter paper No.2, and the filtrate was concentrated under reduced pressure. Water extract was prepared. Then, the ethanol extract was suspended in water, and the same amount of normal hexane was added thereto to fractionate. Then, the same amount of chloroform, the same amount of ethyl acetate, and the same amount of normal butanol were added to the distilled water to sequentially fractionate to obtain each fraction. The schematic diagram of the process of obtaining the extract and each fraction is shown in FIG. 1 together with the yield.

< Experimental example > Whitening activity experiment

< Experimental example  1> Evaluation of melanin synthesis inhibitory ability

Using the melanoma cell line B16F10 derived from C57BL / 6 mice, the melanin synthesis inhibitory ability of the extract was changed.

For cultivation of B16F10 cells, a cell line was cultured at 37 ° C and 5% CO ₂ using DMEM medium containing 10% fetal bovine serum, streptomycin-penicillin (100 mg / mL) and 100 nM α-melanocyte stimulating hormone. Used. As a specific experimental method to see the melanin biosynthesis inhibitory activity, 1 × 10 ⁵ B16F10 cells were dispensed into each well of a 24-well plate and cultured overnight to be used in the experiment. Each sample was cultured for 3 days by treating the medium for each concentration. Then, washed with phosphate-buffered saline and treated with 200 μl of 1N NaOH to dissolve each well. The lysed cells were transferred to a 96 well plate, the optical density was measured at 405 nm using a microplate reader, and the melanin production inhibition rate was calculated as a percentage compared to the negative control group, which was not treated with the sample, and the results are shown in FIGS. 2 to 4.

2 and 3 show the melanin biosynthesis inhibitory activity when the ethanol extract and its fractions were treated at a concentration of 50 μg / ml, and the melanin biosynthesis inhibitory activity by treatment concentration of the ethyl acetate fraction having the highest activity in FIG. 4 was shown. Shown. The ethyl acetate fraction showed melanin biosynthesis inhibitory activity in a concentration-dependent manner and 32.6% inhibitory activity at the highest treatment concentration of 50 μg / ml. Arbutin was used as a positive control.

5 shows the cytotoxicity results by MTT assay, and did not show any particular cytotoxicity even at the highest treatment concentration of 50 μg / ml.

< Experimental example  2> Evaluation of melanin synthesis inhibitory activity in zebrafish

Inhibition of the synthesis of melanin by zebrafish was performed using the ethyl acetate fraction derived from water mud, which showed the highest whitening activity. The mature zebrafish male and female were placed in an egg collection tank the day before the eggs were collected, and the eggs were collected 1-2 hours after the Gwangju period the next day. The collected eggs were placed in a zebrafish embryo medium and generated for 24 hours, and then each sample was treated by concentration. When the sample was processed, the permeability of the sample was not known because the degree of permeation of the sample was not known, and after the sample was processed, the eggs that showed toxicity of the sample after 24 hours were removed, and the remaining eggs were removed. The second sample was processed after complete removal. After 48 hours of sample treatment, the degree of pigment generation in the sample treatment group compared to the control group was observed with a stereomicroscope.

As a result, the pigmentation of the yolk portion of zebrafish treated with 50 µg / ml of the ethyl acetate fraction derived from water quenched as compared to the control (Control) without sample treatment as shown in FIG. It was confirmed that the synthesis of melanin was inhibited.

Claims (6)

A food composition for skin whitening comprising water, ethanol, or a mixed solvent extract of outpost as an active ingredient.
According to claim 1,
Food composition for skin whitening, characterized in that the water-extracted outpost extract is an ethanol extract.
According to claim 1,
The water-exchanged outpost extract is a skin characterized by being a fraction of the ethyl acetate layer obtained by suspending the ethanol extract of water-exchanged outpost in water and then sequentially fractionating using normal hexane, dichloromethane, ethyl acetate and normal butanol. Food composition for whitening.
A cosmetic composition for skin whitening comprising water, ethanol, or a mixed solvent extract of outpost as an active ingredient.
According to claim 4,
The cosmetic composition for skin whitening, characterized in that the water-extracted outpost extract is an ethanol extract.
A pharmaceutical composition for the treatment or prevention of hyperpigmentation of the skin comprising water, ethanol or a mixed solvent extract of the outpost as an active ingredient.

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