KR102268130B1 - Cosmetic Composition comprising Polygonum vellardii All Extract - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising an extract of Polygonum bellardii Alloni, and more specifically, to a cosmetic composition for whitening comprising the extract of Polygonum bellardii Alloni.

Description

Cosmetic composition comprising Polygonum vellardii All Extract

The present invention relates to a cosmetic composition comprising an extract of oleracea.

Several factors are involved in determining a person's skin color and the generation of blemishes and blemishes. Several factors are complexly involved, such as the activity of melanocytes that make melanin, the distribution of blood vessels, the thickness of the skin, carotenoids, and bilirubin. has been Among them, the most important factor is melanin pigment produced by the action of various enzymes in melanocytes in the human body. It is known that genetic factors, hormone secretion, stress, physiological factors such as ultraviolet rays, and environmental factors influence the formation of melanin pigment.

Melanin, which determines a person's skin color, is produced in melanocytes, which are melanocytes. In melanocytes, enzymes such as tyrosinase exist, and they work together to create tyrosine, which is always present in the living body. ) amino acids are polymerized and oxidized to form melanin, a black-brown pigment. The melanin thus formed moves through the dendrites of the melanocytes to the epidermal cells called keratinocytes. Here, melanin forms a hat-like structure around the nucleus to protect genes from UV rays and plays an important role in protecting intracellular proteins by removing free radicals. There is no enzyme that decomposes melanin in the living body, but when keratinocytes age and function and fall off from the epidermis, they are removed from the skin together with keratinocytes. However, when melanin is produced in excess, it causes hyperpigmentation such as spots, freckles, and spots.

Accordingly, studies have been made on whitening agents for inhibiting pigmentation in the skin by preventing excessive production of melanin, inhibitors that inhibit tyrosinase activity such as kojic acid, arbutin, or hydroquinone , vitamin A, vitamin C, a representative antioxidant, and derivatives thereof have been reported.

However, existing whitening substances have weak efficacy or are often accompanied by cytotoxicity, and efforts are being made to discover natural products that are safe and have high whitening efficacy.

In order to solve the above problems, one example of the present invention is to provide a cosmetic composition for whitening comprising a natural extract of Polygonum vellardii All.

Another example of the present invention is to provide a pharmaceutical composition for the prevention or treatment of hyperpigmentation disease, comprising a natural extract of Polygonum vellardii All.

Another example of the present invention is to provide a quasi-drug composition for skin whitening comprising a natural extract of Polygonum vellardii All.

Another example of the present invention is to provide a method for preparing a cosmetic composition for whitening comprising a polygonum vellardii All extract, comprising extracting the polygonum vellardii All with an extraction solvent.

The present invention has completed the present invention by discovering an extract having an effect on skin whitening while searching for substances effective for skin improvement among halophytes in Korea.

An example of the present invention relates to a cosmetic composition for whitening comprising an extract of Polygonum vellardii All.

The large jade knotweed extract may be a solvent extract extracted with one or more selected from the group consisting of water, a linear or branched alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof.

The extract may be a solvent extract extracted with one or more selected from the group consisting of water, methanol, ethanol, propanol, butanol, chloromethane, dichloromethane, diethyl ether, hexane, and ethyl acetate.

The large jade knotweed extract may be a fractional extract obtained by fractionation with a fractionation solvent.

The extract is a fractionated extract obtained by fractionation with a fractionation solvent, and the fractionation solvent is water, a linear or branched alcohol having 1 to 4 carbon atoms, chloromethane, dichloromethane, diethyl ether, hexane, and ethyl acetate. It may be at least one selected from the group.

The large jade knotweed extract may be an ethyl acetate fractional extract obtained by extracting the large jade knotweed with an extraction solvent, and fractionated with ethyl acetate.

The locust japonica extract may be a chloroform fractional extract obtained by sequentially fractionating the locust japonica extract with an extraction solvent, followed by sequential fractionation with ethyl acetate and chloroform.

The extract may be a water residue obtained by sequentially fractionating a solvent extract obtained by extracting the oleander with an extraction solvent with ethyl acetate and chloroform.

The large jade knotweed extract may be extracted by adding an extraction solvent of 1 to 100 times the weight of the large jade knotweed.

The large jade knotweed extract may be extracted with an extraction solvent at a concentration of 50 to 100% (v/v).

The large jade knotweed extract may be included in an amount of 0.0001 to 20% by weight based on 100% by weight of the total composition.

The composition may have a melanin synthesis inhibitory effect.

The composition is more than 33 (%) to 100 (%) or less, 46 (%) to 100 (%) or less, or 54 (%) to more than 100 ( %) or less:

[Equation 1]

Melanogenesis inhibition (%) = [1 - (ODS-ODc)/(ODm-ODc)] × 100

(ODS: Absorbance of K. japonica extract and alpha-MSH-treated sample,

ODm: absorbance of alpha-MSH treated sample,

ODc: absorbance of untreated negative control)

The composition may have a melanin production inhibitory effect of more than 1 to 3 times compared to arbutin.

The composition includes skin, lotion, solution, external ointment, cream, foam, nourishing lotion, flexible lotion, pack, soft water, emulsion, makeup base, essence, soap, detergent, liquid detergent, bath agent, sunscreen cream, sun oil, Suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, foundation, powder foundation, emulsion foundation, wax foundation, patch, and one or more formulations selected from the group consisting of sprays can

Another embodiment of the present invention relates to a pharmaceutical composition for the prevention or treatment of hyperpigmented diseases, comprising an extract of Polygonum vellardii All.

The hyperpigmentation diseases include pigmentation, inflammatory pigmentation, senile pigmentation, gestational pigmentation, melasma, freckles, age spots, blemishes, melanoma, dark circles, spots, melanocytic nevus, nevus ota, nevus blue, and solar nevus. It may be at least one selected from the group consisting of.

The composition may be one or more formulations selected from the group consisting of ointments, creams, gels, lotions, sprays, patches, and sprays.

Another example of the present invention relates to a quasi-drug composition for whitening comprising an extract of Polygonum vellardii All.

Another example of the present invention relates to a method for preparing a cosmetic composition for whitening comprising a polygonum vellardii All extract, comprising extracting the polygonum vellardii All as an extraction solvent.

The step of extracting the large jade knot grass with an extraction solvent may be fractional extraction of the large jade knot grass with a fractionation solvent.

Hereinafter, the present invention will be described in more detail.

An example of the present invention relates to a cosmetic composition comprising an extract of Polygonum vellardii All. The cosmetic composition may be used for whitening.

Polygonum vellardii All is an annual grass distributed in Korea and Japan. It is similar to that of K. velllardii, but it has petioles and has more leaves. Big jade knotweed grows in dry areas of reclaimed land or at the seashore, and is 40-50cm high, with straight stems, thick branches, and long internodes. The leaves are alternate phyllotaxis, the lower part is 3~5cm, the width is 5~7mm, has petiole, and both ends are narrow and long oval or lanceolate, but the upper part is 1cm long and 2mm wide, and the one at the tip is smaller. The leaf sheath is short and has few veins. Flowers bloom in August, hang on the upper leaf axil, peduncles are shorter than flowers, inflorescences are green without opening, and the edges of the upper part are reddish and about 2.5mm long. The fruit is triangular and the upper part protrudes slightly.

The large jade knot grass (Polygonum vellardii All) extract, regardless of the extraction method, the extraction solvent, the extracted component, or the form of the extract, includes all the substances obtained by extracting the components of the large jade knot grass (Polygonum vellardii All), Or, it is a broad concept that includes all that can be obtained by processing or processing the material obtained by extracting the components of the large jade knotweed by other methods after extraction. For example, the extract is an extract obtained by extracting the rhododendron, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a prepared or purified product of the extract, or a mixture thereof, etc., It includes extracts of all formulations that can be formed using the extract itself and the extract. The extract may be an extract obtained by extracting leaves, stems, roots, flowers, and/or outposts, etc.

The method of extracting the large jade knot grass is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a solvent extraction method, hot water extraction method, ultrasonic extraction method, filtration method, reflux extraction method, etc., which may be performed alone or in combination of two or more methods, but is limited thereto no.

The composition according to an exemplary embodiment of the present invention may include an appropriate amount of oleander extract according to the purpose, for example, 0.0001 to 20% by weight, 0.001 to 0.001 to 0.0001 to 20% by weight of the oleander extract based on 100% by weight of the total composition. 20% by weight, 0.002 to 20% by weight, 0.0025 to 20% by weight, 0.0001 to 10% by weight, 0.001 to 10% by weight, 0.002 to 10% by weight, 0.0025 to 10% by weight, 0.0001 to 5% by weight, 0.001 to 5% by weight %, 0.002 to 5% by weight, 0.0025 to 5% by weight, 0.0001 to 1% by weight, 0.001 to 1% by weight, 0.002 to 1% by weight, or 0.0025 to 1% by weight, but is not limited thereto. For example, the composition according to an exemplary embodiment of the present invention may include, but is not limited to, the extract of the oleander extract at a concentration of 20 to 30 ppm.

The kind of extraction solvent used for extracting the large jade knot grass is not particularly limited, and any solvent for extracting the active ingredient from the large jade knot grass may be used. Non-limiting examples of the extraction solvent include water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, and these may be used alone or in combination of one or more. For example, the extraction solvent may be at least one selected from the group consisting of water, a linear or branched alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof, for example, water, methanol, ethanol, propanol, butanol, chloro It may be at least one selected from the group consisting of methane, dichloromethane, diethyl ether, hexane, and ethyl acetate, but is not limited thereto. As an example, the oleander extract according to an embodiment of the present invention may be an ethanol extract of the oleander or an ethanol aqueous solution extract of the oleander.

According to an example of the present invention, the extract of the locust japonica may be a fractional extract obtained by fractionation with a fractionation solvent. The fractional extract refers to a result obtained by performing fractionation in order to separate a specific component or a specific component group from a mixture containing various components.

The fractionation method for obtaining the fraction is not particularly limited, and non-limiting examples of the fractionation method include a solvent fractionation method performed by treating various solvents, ultrafiltration performed by passing through an ultrafiltration membrane having a constant molecular weight cut-off value fractionation methods, chromatographic fractionation methods in which various chromatographies (those designed for separation according to size, charge, hydrophobicity, or affinity) are performed, and combinations thereof, and the like. Specifically, there may be mentioned a method of obtaining a fraction from the extract by treating the extract obtained by extracting the large jade knotweed of the present invention with a predetermined solvent.

The type of the fractionation solvent used to obtain the fraction is not particularly limited, and any solvent for separating and extracting the desired active ingredient from the oleander extract may be used. Non-limiting examples of the fractionation solvent include water, a polar solvent such as an alcohol having 1 to 4 carbon atoms; non-polar solvents such as hexane and ethyl acetate; or a mixed solvent thereof. These may be used alone or in combination of one or more, but is not limited thereto. For example, the fractionation solvent is water, a linear or branched alcohol having 1 to 4 carbon atoms, chloromethane, dichloromethane, diethyl ether, hexane, and ethyl acetate. It may be one or more selected from the group consisting of, but is not limited thereto.

According to an example of the present invention, the oleander extract is an ethyl acetate fractional extract obtained by fractionally extracting a solvent extract obtained by extracting an oleander with an extraction solvent (eg, ethanol or aqueous ethanol solution) with ethyl acetate. can For example, the oleander extract may be an ethyl acetate fractional extract obtained from an ethyl acetate layer by adding ethyl acetate and water to an ethanol extract obtained by adding ethanol or an aqueous ethanol solution to the oleander extract as an extraction solvent. have.

According to another embodiment of the present invention, the locust japonica extract is a chloroform fractionation extract obtained by extracting the locust oleracea with an extraction solvent (eg, ethanol or aqueous ethanol solution), sequentially fractionated with ethyl acetate and chloroform. For example, it may be a fractional extract obtained by fractionating the solvent extract with ethyl acetate and fractionating the residue with chloroform. For example, the oleander extract is after adding ethyl acetate and water to the ethanol extract obtained by adding ethanol as an extraction solvent to the oleander, fractionating into an ethyl acetate layer and a water layer, and the water layer It may be a chloroform fractional extract obtained from the chloroform layer by fractionation after adding chloroform.

According to another example of the present invention, the extract of the oleander may be a water residue obtained by sequentially fractionating a solvent extract obtained by extracting the oleander with an extraction solvent with ethyl acetate and chloroform. For example, the oleander extract is after adding ethyl acetate and water to the ethanol extract obtained by adding ethanol as an extraction solvent to the oleander, fractionating into an ethyl acetate layer and a water layer, and the water layer It may be a water fraction extract obtained from the water layer by fractionation after adding chloroform.

The extract or fraction may be prepared and used in the form of a dry powder after extraction, but is not limited thereto.

The amount of the extraction solvent or the fractionation solvent used may be appropriately selected to extract the active ingredient, for example, 1 to 100 times, 1 to 50 times, 1 to 30 times the weight of the large jade knotweed to be extracted. 1x to 20x, 1x to 15x, 1x to 10x, 1x to 5x, 1x to 3x, 2x to 100x, 2x to 50x, 2x to 30x, 2 times to 20 times, 2 times to 15 times, 2 times to 10 times, 2 times to 5 times, or 2 times to 3 times by weight, 1 of the weight of the extract to be extracted Times to 1000 times, 10 times to 1000 times, 100 times to 1000 times, 1 times to 500 times, 10 times to 500 times, or may be used in a weight of 100 times to 500 times, but is not limited thereto.

The concentration of the extraction solvent or the fractionation solvent may be appropriately selected to extract the active ingredient, for example, 50 to 100% (v/v), 50 to 99.99% (v/v), 50 to 99.9% (v/v), 50 to 99.5% (v/v), 50 to 99% (v/v), 60 to 100% (v/v), 60 to 99.99% (v/v), 60 to 99.9% (v/v), 60 to 99.5% (v/v), 60 to 99% (v/v), 70 to 100% (v/v), 70 to 99.99% (v/v), 70 to 99.9% (v/v), 70 to 99.5% (v/v), 70 to 99% (v/v), 80 to 100% (v/v), 80 to 99.99% (v/v), 80 to 99.9% (v/v), 80 to 99.5% (v/v), 80 to 99% (v/v), 90 to 100% (v/v), 90 to 99.99% (v/v), 90 to 99.9% (v/v), 90 to 99.5% (v/v), 90 to 99% (v/v), 95 to 100% (v/v), 95 to 99.99% (v/v), 95 to 99.9% (v/v), 95 to 99.5% (v/v), or 95 to 99% (v/v), but is not limited thereto.

The cosmetic composition according to an embodiment of the present invention may have a melanin synthesis inhibitory effect. For example, the cosmetic composition comprising the extract of oleracea according to an embodiment of the present invention has a melanin production inhibition degree calculated by the following [Equation 1] is greater than 33 (%) to 100 (%) or less, 34 (%) ) to 100 (%), 35 (%) to 100 (%), 40 (%) to 100 (%), 45 (%) to 100 (%), 46 (%) to 100 (%), 46 (%) ) more than 100 (%) or less, 47 (%) to 100 (%), 50 (%) to 100 (%), more than 54 (%) to 100 (%) or less, 55 (%) to 100 (%) , 56 (%) to 100 (%), 57 (%) to 100 (%), 58 (%) to 100 (%), 59 (%) to 100 (%), 60 (%) to 100 (%) , 60 (%) to 100 (%) or less, 61 (%) to 100 (%), 61 (%) to 100 (%) or less, 62 (%) to 100 (%), 65 (%) to 100 (%), 69 (%) to 100 (%), more than 69 (%) to 100 (%) or less, 70 (%) to 100 (%), 71 (%) to 100 (%), 33 (%) ) more than 100 (%), more than 34 (%) to less than 100 (%), more than 35 (%) to less than 100 (%), more than 40 (%) to less than 100 (%), more than 45 (%) to less than 100 (%), more than 46 (%) to less than 100 (%), more than 46 (%) to less than 100 (%), more than 47 (%) to less than 100 (%), more than 50 (%) to less than 100 (%) Less than (%), more than 54 (%) to less than 100 (%), more than 55 (%) to less than 100 (%), more than 56 (%) to less than 100 (%), more than 57 (%) to less than 100 (%) ) less than, 58 (%) or more to less than 100 (%), 59 (%) or more to less than 100 (%), 60 (%) or more to less than 100 (%), more than 60 (%) to less than 100 (%) , more than 61 (%) to less than 100 (%), more than 61 (%) to less than 100 (%), more than 62 (%) to less than 100 (%), more than 65 (%) to less than 100 (%), 69 (%) or more to less than 100 (%), more than 69 (%) to 10 Less than 0 (%), more than 70 (%) to less than 100 (%), more than 71 (%) to less than 100 (%), more than 33 (%) to less than 99 (%), 34 (%) to less than 99 (%) ), 35 (%) to 99 (%), 40 (%) to 99 (%), 45 (%) to 99 (%), 46 (%) to 99 (%), greater than 46 (%) to 99 ( %) or less, 47 (%) to 99 (%), 50 (%) to 99 (%), more than 54 (%) to 99 (%) or less, 55 (%) to 99 (%), 56 (%) to 99 (%), 57 (%) to 99 (%), 58 (%) to 99 (%), 59 (%) to 99 (%), 60 (%) to 99 (%), 60 (%) More than 99 (%), 61 (%) to 99 (%), more than 61 (%) to 99 (%), 62 (%) to 99 (%), 65 (%) to 99 (%), 69 (%) to 99 (%), more than 69 (%) to 99 (%) or less, 70 (%) to 99 (%), 71 (%) to 99 (%), more than 33 (%) to 95 ( %) or less, 34 (%) to 95 (%), 35 (%) to 95 (%), 40 (%) to 95 (%), 45 (%) to 95 (%), 46 (%) to 95 (%), more than 46 (%) to 95 (%) or less, 47 (%) to 95 (%), 50 (%) to 95 (%), more than 54 (%) to 95 (%) or less, 55 ( %) to 95 (%), 56 (%) to 95 (%), 57 (%) to 95 (%), 58 (%) to 95 (%), 59 (%) to 95 (%), 60 ( %) to 95 (%), more than 60 (%) to 95 (%) or less, 61 (%) to 95 (%), more than 61 (%) to 95 (%) or less, 62 (%) to 95 (%) ), 65 (%) to 95 (%), 69 (%) to 95 (%), more than 69 (%) to 95 (%) or less, 70 (%) to 95 (%), 71 (%) to 95 (%), more than 33 (%) to 90 (%) or less, 34 (%) to 90 (%), 35 (%) to 90 (%), 40 (%) to 90 (%), 45 (%) to 90 (%), 46 (%) to 90 (%), 4 More than 6 (%) to 90 (%) or less, 47 (%) to 90 (%), 50 (%) to 90 (%), more than 54 (%) to 90 (%) or less, 55 (%) to 90 (%) (%), 56 (%) to 90 (%), 57 (%) to 90 (%), 58 (%) to 90 (%), 59 (%) to 90 (%), 60 (%) to 90 (%), more than 60 (%) to 90 (%) or less, 61 (%) to 90 (%), more than 61 (%) to 90 (%) or less, 62 (%) to 90 (%), 65 ( %) to 90 (%), 69 (%) to 90 (%), more than 69 (%) to 90 (%) or less, 70 (%) to 90 (%), 71 (%) to 90 (%), More than 33 (%) to 85 (%) or less, 34 (%) to 85 (%), 35 (%) to 85 (%), 40 (%) to 85 (%), 45 (%) to 85 (%) ), 46 (%) to 85 (%), more than 46 (%) to 85 (%) or less, 47 (%) to 85 (%), 50 (%) to 85 (%), more than 54 (%) to 85 (%) or less, 55 (%) to 85 (%), 56 (%) to 85 (%), 57 (%) to 85 (%), 58 (%) to 85 (%), 59 (%) to 85 (%), 60 (%) to 85 (%), more than 60 (%) to 85 (%) or less, 61 (%) to 85 (%), more than 61 (%) to 85 (%) or less, 62 (%) to 85 (%), 65 (%) to 85 (%), 69 (%) to 85 (%), more than 69 (%) to 85 (%) or less, 70 (%) to 85 (%) ), 71 (%) to 85 (%), more than 33 (%) to 80 (%) or less, 34 (%) to 80 (%), 35 (%) to 80 (%), 40 (%) to 80 (%) (%), 45 (%) to 80 (%), 46 (%) to 80 (%), more than 46 (%) to 80 (%) or less, 47 (%) to 80 (%), 50 (%) to 80 (%), 54 (%) to more than 80 (%), 55 (%) to 80 (%), 56 (%) to 80 (%), 57 (%) to 80 (%), 58 ( %) to 80 (%), 59 (%) to 80 (%), 60 (%) to 80 (%), more than 60 (%) to 80 (%) or less, 61 (%) to 80 (%), more than 61 (%) to 80 (%) or less, 62 (%) to 80 (%) or less (%), 65 (%) to 80 (%), 69 (%) to 80 (%), more than 69 (%) to 80 (%) or less, 70 (%) to 80 (%), 71 (%) to 80 (%), more than 33 (%) to 75 (%) or less, 34 (%) to 75 (%), 35 (%) to 75 (%), 40 (%) to 75 (%), 45 ( %) to 75 (%), 46 (%) to 75 (%), greater than 46 (%) to 75 (%) or less, 47 (%) to 75 (%), 50 (%) to 75 (%), More than 54 (%) to 75 (%) or less, 55 (%) to 75 (%), 56 (%) to 75 (%), 57 (%) to 75 (%), 58 (%) to 75 (%) ), 59 (%) to 75 (%), 60 (%) to 75 (%), more than 60 (%) to 75 (%) or less, 61 (%) to 75 (%), more than 61 (%) to 75 (%) or less, 62 (%) to 75 (%), 65 (%) to 75 (%), 69 (%) to 75 (%), more than 69 (%) to 75 (%) or less, 70 ( %) to 75 (%), 71 (%) to 75 (%), more than 33 (%) to 70 (%) or less, 34 (%) to 70 (%), 35 (%) to 70 (%), 40 (%) to 70 (%), 45 (%) to 70 (%), 46 (%) to 70 (%), more than 46 (%) to 70 (%) or less, 47 (%) to 70 (%) ), 50 (%) to 70 (%), more than 54 (%) to 70 (%) or less, 55 (%) to 70 (%), 56 (%) to 70 (%), 57 (%) to 70 (%), 58 (%) to 70 (%), 59 (%) to 70 (%), 60 (%) to 70 (%), more than 60 (%) to 70 (%) or less, 61 (%) to 70 (%), more than 61 (%) to 70 (%) or less, 62 (%) to 70 (%), 65 (%) to 70 (%), 69 (%) to 70 (%), 69 ( %) more than 70 (%) or less, more than 33 (%) to 65 (%) or less, 34 (%) to 65 (%), 35 (%) to 65 (%), 40 (%) to 65 (%), 45 (%) to 65 (%), 46 (%) ) to 65 (%), more than 46 (%) to 65 (%) or less, 47 (%) to 65 (%), 50 (%) to 65 (%), more than 54 (%) to 65 (%) or less , 55 (%) to 65 (%), 56 (%) to 65 (%), 57 (%) to 65 (%), 58 (%) to 65 (%), 59 (%) to 65 (%) , 60 (%) to 65 (%), more than 60 (%) to 65 (%) or less, 61 (%) to 65 (%), more than 61 (%) to 65 (%) or less, 62 (%) to 65 (%), greater than 33 (%) to less than 61 (%), 34 (%) to less than 61 (%), 35 (%) to less than 61 (%), 40 (%) to less than 61 (%), 45 (%) to less than 61 (%), 46 (%) to less than 61 (%), more than 46 (%) to less than 61 (%), 47 (%) to less than 61 (%), 50 (%) to Less than 61 (%), greater than 54 (%) to less than 61 (%), 55 (%) to less than 61 (%), 56 (%) to less than 61 (%), 57 (%) to less than 61 (%) , 58 (%) to less than 61 (%), 59 (%) to less than 61 (%), or 60 (%) to less than 61 (%), for example, more than 33 (%) to 100 (%) ) or less, or more than 46 (%) to 100 (%) or less.

[Equation 1]

Melanogenesis inhibition (%) = [1 - (ODS-ODc)/(ODm-ODc)] × 100

(ODS: Absorbance of K. japonica extract and alpha-MSH-treated sample,

ODm: absorbance of alpha-MSH treated sample,

ODc: absorbance of untreated negative control)

The cosmetic composition according to an embodiment of the present invention may have a higher melanin production inhibitory effect than arbutin, and thus may have an excellent whitening effect compared to arbutin. For example, the cosmetic composition according to an embodiment of the present invention is more than 1 time to 3 times, 1.01 times to 3 times, 1.05 times to 3 times compared to arbutin, when the active ingredient, oleander extract, is added at a concentration of half that of arbutin. times, 1.1 times to 3 times, 1.15 times to 3 times, 1.2 times to 3 times, 1.25 times to 3 times, 1.3 times to 3 times, 1.35 times to 3 times, 1.4 times to 3 times, 1.45 times to 3 times, 1.5 times to 3 times, more than 1.5 times to 3 times, 1.51 times to 3 times, 1.54 times to 3 times, more than 1 times to 2.5 times, 1.01 times to 2.5 times, 1.05 times to 2.5 times, 1.1 times to 2.5 times, 1.15 to 2.5 times, 1.2 to 2.5 times, 1.25 to 2.5 times, 1.3 to 2.5 times, 1.35 to 2.5 times, 1.4 to 2.5 times, 1.45 to 2.5 times, 1.5 to 2.5 times, 1.5 times. greater than 2.5 times, 1.51 times to 2.5 times, 1.54 times to 2.5 times, more than 1 times to 2 times, 1.01 times to 2 times, 1.05 times to 2 times, 1.1 times to 2 times, 1.15 times to 2 times, 1.2 times to 2 times, 1.25 times to 2 times, 1.3 times to 2 times, 1.35 times to 2 times, 1.4 times to 2 times, 1.45 times to 2 times, 1.5 times to 2 times, more than 1.5 times to 2 times, 1.51 times to It may be one having a melanin production inhibitory effect of 2 times, or 1.54 times to 2 times. For example, the cosmetic composition according to an embodiment of the present invention may have a melanin production inhibitory effect of more than 1 to 3 times compared to arbutin.

Therefore, the composition according to an embodiment of the present invention has a melanogenesis inhibitory effect, and in particular, has a significantly higher melanogenesis inhibitory effect compared to arbutin, so the cosmetic composition according to an embodiment of the present invention may be for whitening.

The whitening means whitening the skin, and inhibits the synthesis of melanin to inhibit or prevent the deposition of melanin on the skin.

The melanin is a pigment of blackish-brown grains, and is made by organelles called melanosomes in melanocytes. Melanin exists in the skin, hair, and eyes, and the skin color is determined by the amount of melanin. The greater the amount of melanin, the darker the skin color.

In an embodiment of the present invention, as a result of culturing by adding the oleander extract to the melanoma cells, melanin production was inhibited as the kombucus oleifera extract was added, thereby confirming the whitening activity of the oleander extract (Figs. 2 and Figs. 3).

Accordingly, the cosmetic composition according to an embodiment of the present invention may include a functional cosmetic having a whitening effect, and the functional cosmetic is a cosmetic that emphasizes the characteristic efficacy and effect as defined in the Cosmetics Act, such as spots, freckles, age spots and blemishes. It may have a preventive or ameliorating effect, but is not limited now, and may include any activity for preventing, improving, or treating pigmentation in the skin.

The prevention refers to any action of suppressing or delaying symptoms by external application or administration of a composition comprising the oleander extract or a fraction thereof according to an embodiment of the present invention.

The improvement refers to any action that reduces a parameter related to a condition to be treated by topical application or administration of the composition, for example, the severity of symptoms.

The cosmetic composition according to an embodiment of the present invention includes skin, lotion, solution, external ointment, cream, foam, nourishing lotion, flexible lotion, pack, soft water, emulsion, makeup base, essence, soap, detergent, liquid detergent, bath agent, from the group consisting of sunscreen creams, sun oils, suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, foundations, powder foundations, emulsion foundations, wax foundations, patches, and sprays. It may be prepared in a selected formulation, but is not limited thereto.

The cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers to be formulated in general skin cosmetics, and common ingredients include, for example, oil, water, surfactant, humectant, lower alcohol, A thickener, a chelating agent, a colorant, a preservative, a fragrance, etc. may be appropriately mixed, but the present invention is not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream or gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro propellants such as, but not limited to, carbon, propane/butane or dimethyl ether. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer, or emulsifier may be used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, and the like can be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, fatty acid ester of polyethylene glycol or sorbitan may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, as a carrier component a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters; Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester may be used, but is not limited thereto. These may be used alone or in combination of two or more.

In the cosmetic composition of the present invention, in addition to the above essential ingredients, other ingredients usually blended in cosmetics may be blended as needed. Specifically, blending ingredients that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, disinfectants, antioxidants, plant extracts, pH adjusters, alcohols, pigments, perfumes, blood circulation promoters, cooling agents, limiting agents, purified water, and the like, but are not limited thereto.

Another embodiment of the present invention relates to a composition for preventing or treating hyperpigmentation disease, comprising an extract of Polygonum vellardii All. The "Keunok knotweed extract" is the same as described above.

The hyperpigmentation disease refers to a disease having symptoms of excessive pigmentation on the skin, and specifically, the hyperpigmentation disease is pigmentation, inflammatory pigmentation, senile pigmentation, gestational pigmentation, melasma, freckles, age spots, It may be one or more selected from the group consisting of blemishes, melanoma, dark circles, spots, melanocyte nevus, nevus ota, blue nevus, and solar nevus, but is not limited thereto.

As used herein, the term “prevention” refers to any action in which the composition of the present invention inhibits or delays the occurrence of hyperpigmented disease.

In the present invention, the term “treatment” refers to any action that allows the pharmaceutical composition of the present invention to improve or benefit the symptoms of hyperpigmentation disease.

The pharmaceutical composition may be one or more formulations selected from the group consisting of ointments, creams, gels, lotions, sprays, patches, and sprays, but is not limited thereto.

The pharmaceutical composition may further include a pharmaceutically acceptable carrier.

The “pharmaceutically acceptable carrier” refers to a carrier or diluent that does not stimulate the organism and does not inhibit the activity and properties of the composition for preventing or treating hyperpigmentation disease symptoms of the present invention. Examples of acceptable pharmaceutical carriers for compositions formulated as liquid solutions are sterile and biocompatible, and include saline, sterile water, Ringer's solution, buffered saline, albumin injection, dextrose solution, maltodextrin solution, glycerol, ethanol. And one or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostats may be added as necessary.

In addition, the pharmaceutically acceptable carrier of the present invention may include a non-natural carrier.

The pharmaceutical composition of the present invention may be administered singly or multiple times in a pharmaceutically effective amount.

In the present invention, the term “pharmaceutically effective amount” means an amount sufficient to prevent or treat a disease at a reasonable benefit/risk ratio applicable to medical prevention or treatment, and the effective dose level depends on the severity of the disease, the activity of the drug. , patient's weight, health, sex, patient's sensitivity to drug, administration time of the composition of the present invention used, administration route and excretion rate, treatment period, factors including the drug used in combination with or concurrently with the composition of the present invention and other factors well known in the medical field.

Another example of the present invention relates to a quasi-drug composition for whitening comprising an extract of Polygonum vellardii All. The "Keunok knotweed extract" is the same as described above.

In the present invention, the term "quasi-drug" refers to articles with a milder action than pharmaceuticals among articles used for the purpose of diagnosing, treating, improving, alleviating, treating or preventing diseases of humans or animals, for example, according to the Pharmaceutical Affairs Act of the Republic of Korea. Quasi-drugs exclude products used for pharmaceutical purposes, and include products used for the treatment or prevention of diseases in humans and animals, and products with minor or no direct action on the human body.

The quasi-drug composition of the present invention may be prepared in a form selected from the group consisting of body cleanser, foam, soap, mask, ointment, cream, lotion, essence and spray, but is not limited thereto.

When the oleander extract or a fraction thereof of the present invention is used as a quasi-drug additive, the quasi-drug extract or a fraction thereof of the present invention can be added as it is or used with other quasi-drugs or quasi-drug ingredients, and can be used appropriately in a conventional method can

In addition, the quasi-drug composition is not particularly limited thereto, and specifically, it may be prepared and used in the form of an ointment, a lotion, a spray, a patch, a cream, a powder, a suspension, a gel, or a gel.

Another example of the present invention relates to a method for preparing a cosmetic composition for whitening comprising a polygonum vellardii All extract, comprising extracting the polygonum vellardii All as an extraction solvent. The step of extracting the large jade knot grass with an extraction solvent may be fractional extraction of the large jade knot grass with a fractionation solvent. The manufacturing method of the cosmetic composition for whitening comprising the extract of the large jade knotweed includes the steps of removing the extraction solvent; and drying may be further included.

For example, the method for producing a cosmetic composition for whitening comprising the extract of oleander may include the step of extracting the oleander with ethanol or an aqueous ethanol solution to obtain an ethanol extract.

For example, the method for producing a cosmetic composition for whitening comprising the extract of the oleander includes the steps of extracting the oleander with an extraction solvent (eg, ethanol or aqueous ethanol solution) to obtain a solvent extract; and fractionation after adding ethyl acetate and water to the solvent extract to obtain an ethyl acetate fractional extract from the ethyl acetate layer.

For example, the method for producing a cosmetic composition for whitening comprising the extract of the oleander includes the steps of extracting the oleander with an extraction solvent (eg, ethanol or aqueous ethanol solution) to obtain a solvent extract; and fractionating after adding ethyl acetate and water to the solvent extract, followed by fractionation into an ethyl acetate layer and a water layer; and fractionation after adding chloroform to the water layer to obtain a chloroform fraction extract and a water fraction extract.

As another aspect for achieving the object of the present application, the present application provides a method for whitening the skin, comprising administering to an individual in need thereof, an extract, or a fraction thereof.

In the method for whitening the skin of the present application, the extract or fractions thereof and whitening are as described above.

The extract or fractions thereof of the present application may be administered to an individual in need thereof in an effective amount.

The level of the effective dose can be determined by a person skilled in the art based on common sense to the extent that the desired skin whitening effect appears.

As another aspect for achieving the object of the present invention, it provides a method for preventing or treating hyperpigmented disease, comprising administering an extract or a fraction thereof to an individual in need thereof.

In the method of whitening the skin of the present application, the extract or its fractions and administration are as described above.

The extract or fractions thereof of the present application may be administered to an individual in need thereof in an effective amount. The level of the effective dose can be determined by those skilled in the art based on common common sense to the extent that the effect of preventing or treating hyperpigmentation is shown.

The composition comprising the oleander extract according to the present invention can effectively inhibit melanin synthesis and thus help skin whitening, thereby preventing and improving skin color change due to physiological and environmental factors. The composition comprising the extract according to the present invention can be safely used from toxicity or side effects by using a natural salt produced in Korea.

1 is a view showing the MTT assay results of the extract according to an example of the present invention.
Figure 2 is a view showing the degree of inhibition (%) of melanin production of the large jade knotweed solvent extract according to an example of the present invention.
Figure 3 is a view showing the degree of inhibition (%) of melanin production of the extract of the locust japonica fraction according to an example of the present invention.

Hereinafter, to help the understanding of the present invention, examples will be described in detail. However, the following examples are merely illustrative of the contents of the present invention, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

Example 1. Preparation of extracts from keunok knotweed

(1) Solvent extraction of locust knotweed

Polygonum vellardii All, which grows wild in Korea, was collected in September 2018 from Gyeonggi-do, Ganghwa-do, and Donggum-do and dried outposts. Then, 1.6817 kg of outposts were used in 99.9% (v/v) ethyl alcohol 4 It was put into L and extracted for 3 days at room temperature. Thereafter, the extract was filtered using a non-fluorescent cotton filter, and concentrated with a rotary evaporator (N-1000SWD; EYELA) at 40° C., and 24 hours using a module spin 40 (Biotron co.). During drying, an ethanol extract (Example 1-1) was obtained. After the drying process, the extract was stored in a cold room at a temperature of -4 ℃ after removing the solvent in the form of 20 mg/tube.

(2) Extraction of the fraction of the locust weed

The ethanol extract prepared in (1) of Example 1 was dissolved in 50 ml of ethyl alcohol, and the salt of the extract was removed using a vacuum extraction filter and a flask. After dissolving 3.31 g of the salt-removed extract in 1.5 L of a mixed solvent of 1:1 ratio of ethyl acetate and water, it was placed in a separatory funnel and fractionated into an ethyl acetate layer and an aqueous layer. Chloroform was added to the water layer again in a volume ratio of 1:1, and then put into a separatory funnel to fractionate the chloroform layer and the water layer.

The ethyl acetate fraction obtained above, the chloroform fraction, and the water fraction, respectively, of the oleander were concentrated using a rotary evaporator; spin 40; Biotron co.) and dried for 24 hours to extract (Example 1-2), chloroform fraction extract (Example 1-3) and water fraction extract (Examples 1-4) were obtained, respectively.

After the drying process, after removing the solvent in the form of 20 mg/tube, each of the extracts of K. japonica was stored in a low temperature room at a temperature of -4°C below zero.

Example 2: Measurement of the cytotoxicity of the extract

(1) Solvent extract of locust japonica

Cytotoxicity was measured with respect to the solvent extract of K. japonica by using the MTT assay.

Specifically, after dispensing 600 μL of B16F10 melanoma cells (ATCC, USA) ^{in a 48-well plate at 2 x 10 4} cells/well, incubated overnight in an incubator, DMEM (Dulbecco's Modified Eagle's Medium) high glucose Medium (ATCC, USA) was used. The solvent extract prepared in (1) of Example 1 was dissolved in Alpha-MSH (0.4 uM, Sigma-Aldrich, USA), which is a stimulating factor for promoting melanin production, and DMSO, with a concentration of 25 ppm A medium containing Example 1-1) was prepared. The medium in the dispensed melanoma cell wells was removed, and 600 μL of the prepared medium containing 25 ppm of the ethanol extract of oleracea was added per well. As a positive control, 50 ppm of arbutin (Comparative Example 1) was used.

After culturing melanoma cells in a 37° C. CO ₂ incubator for 24 hours, the dispensed well medium and samples were removed, treated with 250 μL of 0.5 mg/ml MTT, and incubated for 2 hours in an incubator while blocking light. After that, the medium was removed and incubated on a shaker for 30 minutes in a state treated with 300 μL of DMSO. Then, 200 μL of the supernatant was transferred to 96 wells and measured by ELISA at a wavelength of 540 nm. The results of cytotoxicity measured by ELSIA are shown in FIG. 1 .

As shown in FIG. 1 , it was confirmed that the K. japonica extract had no cytotoxicity. In particular, as a result of MTT assay, the absorbance value of the negative control group was 80 or higher based on 100, confirming that it was applicable to various diseases and conditions.

Example 3. Confirmation of whitening activity of Keunok knotweed extract

(1) Solvent extract of locust japonica

A sample of 20 mg/tube of the oleander extract prepared in (1) of Example 1 was dissolved with dimethyl sulfoxide (DMSO) to a concentration of 10 mg/ml for use in cell experiments, and this was It was diluted with a culture medium and used for the experiment.

B16F10 melanoma cells (ATCC, USA) ^{were aliquoted at a concentration of 2x10 4} cells/well in a 48-well plate by 600 μL and cultured overnight in an incubator. As the medium, DMEM (Dulbecco's Modified Eagle's Medium) high glucose medium (ATCC, USA) was used.

A 25ppm medium was prepared by dissolving the Melanin production stimulating agent Alpha-MSH (0.4 uM, Sigma-Aldrich, USA) and DMSO prepared in Example 1 (1). As a positive control, arbutin 50ppm (Comparative Example 1) was used.

After removing the medium in the wells in which the melanoma cells were seeded, 600 μL of the prepared medium containing the oleander extract was added per well. After 72 hours, the medium was completely removed, 200 μL of 1N NaOH was added per well, and then incubated at 60° C. for 1 hour to dissolve melanin from the cells. 100 μL of the dissolved melanin sample was transferred to a 96-well plate and absorbance was measured at 405 nm in a microplate reader (Molecular Devices, Sunnyvale, CA, USA). From the difference in absorbance, the degree of inhibition of melanin production was calculated as in Equation 1 below. The measurement result of melanin production inhibition is shown in FIG. 2 .

[Equation 1]

Melanogenesis inhibition (%) = [1 - (ODS-ODc)/(ODm-ODc)] × 100

(ODS: Absorbance of the sample treated with the oleander extract and alpha-MSH,

ODm: absorbance of alpha-MSH treated sample,

ODc: Absorbance of K. japonica extract and alpha-MSH untreated negative control (1N NaOH 100μL))

As shown in FIG. 2 , the extract prepared in (1) of Example 1 had an inhibitory effect on melanin production, and in particular, even at a concentration twice lower than that of arbutin (50 ppm), which is a positive control, higher melanin production than arbutin. It showed inhibitory activity. Specifically, when considering the amount of medium added, the oleander extract had a melanin production inhibitory activity of more than 1 to 2 times, even though it was added at a concentration of 0.5 times than that of the positive control arbutin, for example, Although the ethanol extract was added at half the concentration of the positive control arbutin, it showed about 1.12 times more melanin production inhibitory activity.

According to the above results, the melanin production inhibitory activity of the oleander extract is very excellent, and the oleander extract according to an embodiment of the present invention improves various diseases and conditions related to skin pigmentation through inhibition of melanin production and It was confirmed that it can be applied to treatment.

(2) Fractionated extract of K. japonicum grass

It was carried out in substantially the same manner as in Example 3 (1), but using the large jade knotweed fraction extract (Examples 1-2 to 1-4) prepared in (2) of Example 1 as an extract It was confirmed that the whitening activity of the extract of the K. japonica fraction extract. The degree of inhibition of melanin production was calculated according to Equation 1 and is shown in FIG. 3 .

As shown in FIG. 3 , the extract prepared in (2) of Example 1 had an inhibitory effect on melanin production, and in particular, even at a concentration twice lower than that of arbutin (50 ppm), which is a positive control, higher melanin than arbutin. It showed production inhibitory activity. Specifically, when considering the amount of the medium added, the oleander extract had a melanin production inhibitory activity of more than 1 to 2 times, even though it was added at a concentration of 0.5 times that of the positive control arbutin, for example, Although the ethyl acetate fractional extract (Example 1-2) was added at half the concentration of arbutin in the positive control group, it was about 1.5 times or more, and the chloroform fraction extract (Example 1-3) was half the concentration of arbutin as a positive control. In spite of the addition of about 1.3 times or more, the water fraction extract (Example 1-4) of K. japonica showed melanin production inhibitory activity of about 1.54 times or more, even though it was added at half the concentration of arbutin, a positive control.

According to the above results, the melanin production inhibitory activity of the oleander extract is very excellent, and the oleander extract according to an embodiment of the present invention improves various diseases and conditions related to skin pigmentation through inhibition of melanin production and It was confirmed that it can be applied to treatment.

So far, specific embodiments of the present invention have been described. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in modified forms without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated by the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (25)

A cosmetic composition for whitening comprising an extract of Polygonum vellardii All. The cosmetic composition according to claim 1, wherein the extract is a solvent extract extracted with one or more selected from the group consisting of water, a linear or branched alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof. The solvent extract according to claim 1, wherein the extract of K. japonica is selected from the group consisting of water, methanol, ethanol, propanol, butanol, chloromethane, dichloromethane, diethyl ether, hexane, and ethyl acetate. Phosphorus, a cosmetic composition. The cosmetic composition according to claim 1, wherein the extract of the oleander is a fractionated extract obtained by fractionation with a fractionation solvent. The method according to claim 1, wherein the extract is a fractionated extract obtained by fractionation with a fractionation solvent, and the fractionation solvent is water, a linear or branched alcohol having 1 to 4 carbon atoms, chloromethane, dichloromethane, diethyl ether, hexane. , And at least one selected from the group consisting of ethyl acetate, a cosmetic composition. The cosmetic composition according to claim 1, wherein the extract of oleracea is an ethyl acetate fractional extract obtained by extracting the oleander with an extraction solvent, and fractionation with ethyl acetate. The cosmetic composition according to claim 1, wherein the extract is a chloroform fraction obtained by sequentially fractionating a solvent extract obtained by extracting a locust oleracea with an extraction solvent with ethyl acetate and chloroform. The cosmetic composition according to claim 1, wherein the extract of K. japonica is a water residue obtained by sequentially fractionating the solvent extract obtained by extracting the K. japonica with an extraction solvent with ethyl acetate and chloroform. The cosmetic composition according to claim 1, wherein the extract is extracted by adding an extraction solvent in an amount of 1 to 100 times the weight of the large jade knotweed. The cosmetic composition according to claim 1, wherein the extract of the oleander extract is extracted with an extraction solvent at a concentration of 50 to 100% (v/v). The cosmetic composition according to claim 1, wherein the extract of the large jade knotweed is included in an amount of 0.0001 to 20% by weight based on 100% by weight of the total composition. The cosmetic composition according to claim 1, wherein the composition has an inhibitory effect on melanin synthesis. The cosmetic composition according to claim 1, wherein the composition has a degree of inhibition of melanogenesis calculated by the following [Equation 1] of 33 (%) to 100 (%) or less:
[Equation 1]
Melanogenesis inhibition (%) = [1 - (ODS-ODc)/(ODm-ODc)] × 100
(ODS: Absorbance of K. japonica extract and alpha-MSH-treated sample,
ODm: absorbance of alpha-MSH treated sample,
ODc: absorbance of untreated negative control) The cosmetic composition according to claim 1, wherein the composition has a degree of inhibition of melanogenesis calculated by the following [Equation 1] of 46 (%) to 100 (%) or less:
[Equation 1]
Melanogenesis inhibition (%) = [1 - (ODS-ODc)/(ODm-ODc)] × 100
(ODS: Absorbance of K. japonica extract and alpha-MSH-treated sample,
ODm: absorbance of alpha-MSH treated sample,
ODc: absorbance of untreated negative control) The cosmetic composition according to claim 1, wherein the composition has a melanogenesis inhibitory effect of more than 1 to 3 times compared to arbutin. According to claim 1, wherein the composition is skin, lotion, solution, external ointment, cream, foam, nourishing lotion, softening lotion, pack, soft water, emulsion, makeup base, essence, soap, detergent, liquid detergent, bath agent, sun selected from the group consisting of screen creams, sun oils, suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, foundations, powder foundations, emulsion foundations, wax foundations, patches, and sprays. One or more formulations, a cosmetic composition. A pharmaceutical composition for the treatment of hyperpigmented diseases comprising an extract of Polygonum vellardii All. 18. The method of claim 17, wherein the hyperpigmented disease is pigmentation, inflammatory pigmentation, senile pigmentation, gestational pigmentation, melasma, freckles, age spots, blemishes, melanoma, dark circles, spots, melanocyte nevus, nevus ota, blue color At least one selected from the group consisting of nevus, and solar surplus, a pharmaceutical composition. The pharmaceutical composition of claim 17, wherein the composition is one or more formulations selected from the group consisting of ointments, creams, gels, lotions, sprays, patches, and sprays. A quasi-drug composition for whitening comprising an extract of Polygonum vellardii All. A method of manufacturing a cosmetic composition for whitening comprising a polygonum vellardii All extract, comprising the step of extracting the polygonum vellardii All with an extraction solvent. The method according to claim 21, wherein the step of extracting the large jade knot grass with an extraction solvent is to fractionally extract the large jade knot grass with a fractionation solvent. A pharmaceutical composition for preventing hyperpigmentation comprising an extract of Polygonum vellardii All. The method of claim 23, wherein the hyperpigmented disease is pigmentation, inflammatory pigmentation, senile pigmentation, gestational pigmentation, melasma, freckles, age spots, blemishes, melanoma, dark circles, spots, melanocyte nevus, nevus ota, blue At least one selected from the group consisting of nevus, and solar surplus, a pharmaceutical composition. The pharmaceutical composition of claim 23, wherein the composition is one or more formulations selected from the group consisting of ointments, creams, gels, lotions, sprays, patches, and sprays.

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