JP2014221754A - Cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a natural product-derived cosmetic which is excellent in biological safety, has a skin-beautifying effect and a skin-whitening effect of maintaining the skin of a user in a young and healthy state or of capable of improving the skin, and contains an active ingredient having an effect of preventing and improving damage to the skin by external environmental factors including ultraviolet rays and chemical substances.SOLUTION: A cosmetic contains an extract which has an anti-inflammatory effect, an effect of inhibiting primary irritation of the skin of a user, and an antioxidant effect as an active ingredient, and which is obtained from an extract of Prunus armeniaca Linne var. ansu Maximowicz or Prunus armeniaca Linne, or its relative Prunus salicina or Prunus mume. The cosmetic has effects: an effect of preventing and improving skin aging (wrinkles, sags, etc.); an effect of enhancing the tension and gloss of the skin; an effect of preventing and improving oxidation damage to the skin, inflammation of the skin, and skin irritation by external environmental factors (ultraviolet rays and chemical substances); and an effect of preventing and improving spots, freckles, chloasma, etc.

Description

  The present invention is a cosmetic that is obtained from apricot (Prunus armeniaca Linne var anus Maximowicz) or honan (Prunus armeniaca Linne), has excellent biosafety, and has an excellent skin and whitening effect. The present invention relates to a blended component and a cosmetic comprising such a component.

  Skin aging is induced by internal factors such as inactivation of cell growth and differentiation, hormonal secretion, and quantitative reduction of extracellular matrix components, and sunlight (ultraviolet rays) and various chemical substances with aging. This is a phenomenon caused by complicated intertwining with external factors such as cell / tissue damage or inflammation. Skin aging manifests itself as wrinkles, tarmi formation, reduced skin elasticity, spots, freckles, and melasma.

  In order to prevent this skin aging and keep the skin healthy and youthful, the use of various active ingredients has been proposed in the past, and cosmetics containing these active ingredients (beautifying skin ingredients, whitening ingredients, etc.) have been proposed. It is on the market. For example, vitamin C, vitamin E, superoxide dismutase (hereinafter abbreviated as SOD), an antioxidant such as catalase; an anti-inflammatory agent such as glycyrrhizic acid; various ultraviolet absorbers; α-hydroxycarboxylic acid, placenta extract Cell activation components such as γ-amino-β-hydroxybutyric acid; extracellular matrix components such as collagen, elastin and hyaluronic acid; and humectants such as urea. Further, arbutin, kojic acid, and the like are known as substances that suppress the occurrence of pigmentation such as skin spots, buckwheat, and melasma, and are widely used as active ingredients for whitening agents.

  As described above, skin beautifying agents and whitening agents have been proposed based on various skin aging phenomenon mechanisms. However, the safety of skin and the like, and the effectiveness when actually applied to the skin, have been proposed. There is a problem in terms of perspective.

Furthermore, in recent years, there has been a demand for cosmetic ingredients that demonstrate the effectiveness of preventing and improving skin damage caused by various external environmental factors (ultraviolet rays, chemical substances, house dust, pollen). The ingredients that are satisfactory are not yet known.

  In view of the problems of the prior art, the present inventors are natural skin-derived and whitening ingredients derived from natural products from the viewpoint of skin safety, and ingredients that can protect the skin from various external environmental factors. We conducted earnest research to find out. As a result, the extract of Prunus armeniaca Linne var anus Maximowicz or Honzu (Prunus armeniaca Linne) has an anti-inflammatory action, a primary skin irritation inhibitory action, and an antioxidant action, and their synergy. It is possible to provide cosmetics that have an excellent skin beautifying effect and whitening effect by blending the extract, and prevent and improve skin damage caused by external environmental factors. I found.

  Conventionally, head cosmetics (patent document 1), protease inhibitors (patent document 2), bath preparations (patent document 3), and antiallergic agents (patent document 4) blended with an apricot extract of the genus Rosaceae are used. Proposed.

Japanese Patent Laid-Open No. 03-188013 JP 10-338642 A JP 2000-186031 A Japanese Patent Laid-Open No. 2001-064192

  However, conventionally, an apricot extract has an anti-inflammatory action, an inhibitory action on primary skin irritation, and an anti-oxidant action. It has not been known that it has an effect of preventing and improving skin damage caused by external environmental factors.

The present invention is a cosmetic composition containing an extract of Prunus armeniaca Linne var anus Maximowicz or Phonus armeniaca Linne as an active ingredient.
In the present invention, it is preferable to use apricot fruit.
In the present specification, the term cosmetic is used in a broad sense including so-called cosmetics and quasi-drugs.

The present invention is a cosmetic comprising, as an active ingredient, an extract of Rosaceae (Prunus armeniaca Linne var anus Maximowicz) or Honzu (Prunus armeniaca Linne), which is a plant of the Rosaceae family, Has anti-inflammatory action (suppression of degranulation), primary skin irritation prevention action, and anti-oxidation action, and synergistic action to prevent aging of skin wrinkles, tarmi, etc., improvement of skin firmness and gloss Further, it is possible to provide a cosmetic having an effect of preventing and improving pigmentation such as spots, freckles, and melasma, and an effect of preventing and improving skin damage caused by external environmental factors.

FIG. 1 is a diagram showing the degranulation inhibitory effect of an apricot extract according to the present invention. FIG. 2 is a diagram showing the DPPH radical scavenging effect of the apricot extract according to the present invention. FIG. 3 is a diagram showing the SOD-like activity effect of the apricot extract according to the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail.
The extraction material used in the present invention is an apricot (Prunus armeniaca Linne var anus Maximowicz) or apricot (Prunus armeniaca Linne) of any variety (variant or subspecies, or hybrid). Also good. Moreover, the plum (Prunus salicina) or the ume (Prunus mume) which are related plants of apricot may be sufficient.

  The material used in the present invention may be any of apricot, hon apricot, plum or ume whole plant, fruit, pericarp, leaf, flower part, stem, seed, root, etc. Is preferred. The time and size of use of apricot, hon-anzu, plum or plum are not particularly limited, and any size and time of collection may be used.

  For the preparation of the extract, first, if necessary, the apricot, hon-anzu, plum or ume use site (for example, whole grass, flower part and / or fruit) is washed with water in advance to remove foreign substances and then left as it is or dried. In addition, if necessary, it is shredded or pulverized or made into a paste and extracted by contacting with an extraction solvent. The extraction can be performed by bringing the material into contact with an extraction solvent according to a conventional method such as an immersion method, but a supercritical extraction method or a steam distillation method can also be used.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol, propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin; ethyl acetate, butyl acetate, methyl propionate, etc. Esters; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether and isopropyl ether; Hydrocarbon solvents such as n-hexane, toluene and chloroform, and the like. These may be used alone or in combination of two or more. Used.

  Among these extraction solvents, the anti-inflammatory action, the primary skin irritation suppressing action and the antioxidant action of the obtained extract, and also the wide application to cosmetics is possible in the present invention. Hydrophilic solvents such as lower alcohols or polyhydric alcohols are preferred. Preferable examples of using this hydrophilic solvent include, for example, water, lower alcohols (especially ethanol), or polyhydric alcohols (especially 1,3-butylene glycol) alone, or water and lower alcohols. Examples include the use of a mixed solvent with (especially ethanol) or a mixed solvent of water and a polyhydric alcohol (especially 1,3-butylene glycol, glycerin). Among them, water alone or water with 1,3 -A mixed solvent of butylene glycol is particularly preferred.

  When the mixed solvent is used, for example, if the mixed solvent is water and 1,3-butylene glycol, the volume ratio (hereinafter the same) is 1: 1 to 20: 1, and the mixed solvent is water and ethanol. If it exists, it is preferable to set it as the range of 1: 1 to 20: 1 if it is 1: 1 to 25: 1 and the mixed solvent of water and glycerol.

  The weight ratio of the apricot material to the extraction solvent is preferably in the range of 1:50 to 50: 1, more preferably in the range of 1: 1 to 1:35.

  In preparing the extract, the pH is not particularly limited, but it is generally preferably in the range of 3-9. In this sense, if necessary, the extraction solvent is blended with an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid. You may adjust so that it may become pH of.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, water or 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent. The extraction temperature is preferably in the range of 0 ° C. to 90 ° C., more preferably in the range of 0 ° C. to 80 ° C., and the extraction time is preferably 1 to 168 hours (1 hour to 1 week). More preferably, it is in the range of 1 to 120 hours (1 hour to 5 days).

  In addition, you may perform a hydrolysis process to apricot as needed prior to the extraction process of this invention, or in parallel with an extraction process. As a result, the storage stability of the apricot, hon-anzu, plum or ume extract may be improved, and the extract as a cosmetic preparation may be used more effectively.

  In the case of subjecting the extract to an enzymatic hydrolysis treatment, examples of the enzyme include proteolytic enzymes such as actinase, papain and pepsin, starch degrading enzymes such as glucoamylase, α-amylase and β-amylase, cellulase, hemicellulase and pectinase. One or two or more selected from the group of enzymes of lipolytic enzymes such as fibrinolytic enzymes and lipases may be used, but one or more selected from these enzyme groups, respectively. It is more preferable to use a combination of these enzymes.

  The amount of the enzyme added is, for example, in the range of 0.01 to 10% by weight with respect to the solid content of whole apricot, honzu, plum or plum, or flower and / or fruit. More preferably, it is in the range of 0.1 to 2.0% by weight.

  In general, the extract prepared as described above may be adjusted to a pH of 4 to 8 and used as it is as a cosmetic compounding agent as it is or as a desired concentration by vacuum concentration or the like. You may do it. In addition, the extract may be dried by a conventional method such as spray drying.

  In addition, the extract prepared as described above may be refrigerated for a certain period of time in order to enhance storage stability and the like, and the supernatant may be used as a cosmetic compounding agent.

  Examples of cosmetics (including quasi-drugs) containing the apricot, hon-anzu, plum or ume extract of the present invention include basic cosmetics such as emulsions, creams, lotions, essences and packs, lipsticks, foundations and liquid foundations. Makeup cosmetics such as makeup press powder, blusher and white powder, facial cleansers, body shampoos, hair shampoos, soaps and other cleansing cosmetics, hair restorers, and bath preparations. It is not limited to.

  The amount of the extract of apricot, hon apricot, plum or ume in the cosmetic of the present invention is generally 0.002 to 1.0% by weight, preferably 0.00% in the case of a basic cosmetic, as the solid content of the extract. In the range of 02 to 0.2% by weight, in the case of makeup cosmetics, generally in the range of 0.002 to 1.0% by weight, preferably in the range of 0.02 to 0.2% by weight, and in the case of cleaning cosmetics Is generally in the range of 0.002 to 10.0 wt%, preferably 0.02 to 7.0 wt%.

  In addition to essential apricot, honzu, plum and ume extracts, the cosmetics of the present invention include components usually used in cosmetics, such as oily ingredients, surfactants (synthetic and natural products), moisturizing agents. Agents, thickeners, emulsifiers or emulsifying aids, antiseptic / bactericidal agents, powder components, ultraviolet absorbers, antioxidants, pigments, fragrances, other physiologically active components, and the like can be appropriately blended as necessary. . Further, as long as the effectiveness and features of the apricot extract of the present invention are not impaired, it may be combined with other physiologically active ingredients in cosmetics.

Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

As emulsifiers or emulsifiers, stevia derivatives such as enzyme-treated stevia, lecithin and derivatives thereof, lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, beans, millet, etc.) and the like can also be blended.

Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) Examples include extracts, various amino acids, and derivatives thereof.

Examples of the thickener include components derived from brown algae, green algae or red algae such as alginic acid, agar, carrageenan and fucoidan; silane root (white) extract; polysaccharides such as pectin, locust bean gum and aloe polysaccharide; xanthan gum, Gums such as tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives; Examples include glutamic acid and its derivatives.

Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Luconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), 1,2-pentanediol, various essential oils, bark dry matter, and the like.

Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powders, powders of cereals (rice, wheat, corn, millet, etc.), powders of beans (soybeans, red beans, etc.).

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (eg, vitamin E nicotinate, vitamin E linoleate, etc.), murasakixikib extract, peony extract, silane root (white and white) ) There are extracts, etc.

Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof, 4 -Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), 4-HPB (rhodenol, 4- (4-hydroxyphenyl) -4-butanol)), hydroxybenzoic acid And derivatives thereof, vitamin E and derivatives thereof, α-hydroxy acid, and AMP (adenosine monophosphate, adenosine monophosphate). These may be used alone or in combination.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, ascorbic acid sugar derivatives such as L-ascorbic acid-5-glucoside, 6-position acylated products of these ascorbic acid sugar derivatives (acyl group is Hexanoyl group, o L-ascorbic acid tetrafatty acid esters such as L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid- 2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or acylated derivatives thereof, ascorbyl glycerol derivatives such as bisglyceryl ascorbic acid, as hydroquinone derivatives, arbutin (hydroquinone-β-D-glucopyranoside), α-Arbutin (hydroquinone-α-D-glucopyranoside) and the like as tranexamic acid derivatives include tranexamic acid esters (for example, tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranet And amides of tranexamic acid (for example, tranexamic acid methylamide). Resorcinol derivatives include, for example, 4-n-butylresorcinol, 4-isoamylresorcinol, and the like. Examples of the -dihydroxybenzoic acid derivative include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid. Examples of the nicotinic acid derivative include nicotinic acid amide. Examples of the α-hydroxy acid such as benzyl nicotinate include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

As a physiologically active ingredient, as a whitening ingredient, for example, placenta extract, Sakuhakuhi extract, yukinoshita extract, perilla extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, white coconut Fermented product, peony extract or hydrolyzate thereof, lactic acid bacteria fermented rice, murasakixikib extract, lotus seed extract or hydrolyzate thereof, lotus seed fermented product, party extract, pearl hydrolyzed product, pearl seed fermented product, royal jelly Fermented product, fermented sake lees, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract, etc. Coral grass extract as an anti-aging component , Rice leaf extract or hydrolyzate thereof, eggplant (water eggplant, long eggplant, Kamo eggplant, rice eggplant etc.) extract or water Extract, seaweed extract such as catamen giraffe, extract of marine flowering plant such as sea bream, fermented soymilk, damask rose extract, jellyfish water, rice extract or hydrolyzate thereof, rice fermentation extract, germinated rice Extract or hydrolyzate thereof, germinated rice fermented product, black bean extract or hydrolyzate thereof, linoleic acid and derivatives or processed products thereof (for example, liposomal linoleic acid), animal or fish-derived collagen and derivatives thereof, elastin And derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and derivatives thereof, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract Carrot extract, aloe extract, beetroot extract, loofah extract, ana Reed extract, Juazeiro (Zizyphus joazeiro) have extracts.

  Next, the present invention will be described more specifically with reference to production examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of Hon Anzu Fruit Extract Solution The pericarp and seeds were removed from Hon Anzu fruit of the Rosaceae family, and made into a paste using a grinder. To 90 g of this fruit paste, 210 g of 1,3-butylene glycol was added and extracted at 4 ° C. To this was added 410 g of purified water, followed by filtration to obtain 587 g of a brown transparent honan fruit extract solution (solid content concentration: 1.68%).

Production Example 2 Preparation of apricot fruit extract solution Seeds were removed from apricot fruit of the Rosaceae genus, and made into a paste using a grinder. To 90 g of this fruit paste, 210 g of 1,3-butylene glycol was added and extracted at 4 ° C. 410 g of purified water was added thereto, followed by filtration to obtain 580 g of a brown apricot fruit extract solution (solid content concentration 1.61%).

Production Example 3 Preparation of apricot blossom extract solution Apricot blossoms of Rosaceae were dried, and 22.5 g of water was added to 1.5 g of the dried flower and extracted at 80 ° C. This was filtered to obtain 16 g of a brown apricot flower extract solution (solid content concentration 2.46%).

Production Example 4 Preparation of Hon Anzu Flower Extract Solution After drying Honza flowers of the family Rosaceae, 15 g of water is added to 1 g of the dried flower and immersed at 4 ° C., and then 15 g of 1,3-butylene glycol is added. Extracted at 4 ° C. This was filtered to obtain 26 g of brown apricot fruit extract solution (solid content concentration 1.05%).

Production Example 5 Preparation of whole apricot extract of horn apricot The whole honey apricot of the family Rosaceae is genus, and 150 g of water is added to 10 g of this whole herb dried product and immersed at 4 ° C., and 1,3-butylene glycol is added thereto. After adding 150 g, extraction was performed at 4 ° C. This was filtered to obtain 146 g of a brown apricot whole plant extract solution (solid content concentration: 1.78%).

Formulation Example 1 Lotion [A component] part olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[B component]
Extract solution of Production Example 1 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide appropriate amount Purified water Amount that makes the total amount 100 parts [component C]
Fragrance Appropriate amounts of component A and component B were each heated to 80 ° C. or higher, component B was added to component A and stirred, and then homogenized for 2 minutes with Hiscotron (5000 rpm). After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Formulation Example 2 A lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the extract solution in Production Example 2 was used instead of the extract solution in Production Example 1 contained in the B component of Formulation 1. .

Formulation Example 3 A lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the extract solution in Production Example 3 was used instead of the extract solution in Production Example 1 contained in Component B of Skin Lotion Formulation Example 1. .

Formulation Example 4 A lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract solution in Production Example 4 was used instead of the extract solution in Production Example 1 contained in the B component of the lotion formulation Example 1. .

Formulation Example 5 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[B component]
Extract solution of Production Example 1 3.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Formulation Example 6 Emulsion Emulsion in the same manner as in Formulation Example 5 except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. Got.

Formulation Example 7 Emulsion In the component B of Formulation Example 5, the emulsion was prepared in the same manner as Formulation Example 5 except that 3.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. Obtained.

Formulation Example 8 Emulsion In the same manner as in Formulation Example 5, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. An emulsion was obtained.

Formulation Example 9 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[B component]
Extract solution of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Arbutin 3.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts

Formulation Example 10 Essence [ingredient] part ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Extract solution of Production Example 5 5.0
Citric acid 0.3
Sodium citrate 0.6
Purified water After hyaluronic acid was dissolved in purified water in an amount of 100 parts, the remaining raw materials were sequentially added and dissolved by stirring to obtain a transparent essence.

Example 11 Liquid foundation [component A] part stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[Component B] Extract solution of Part Production Example 1 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount of 100 parts [component C]
Titanium oxide 8.0
Talc 4.0
Coloring pigment Appropriate amounts The above-mentioned components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Formulation Example 12. Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B component]
Extract solution of Production Example 2 5.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Example 13 Hair shampoo [component A] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[Component B] part citric acid 0.1
Extract solution of Production Example 1 5.0
1,3-butylene glycol 2.0
Purified water Amounts of 100 parts in total A and B components were each heated to 80 ° C and dissolved uniformly, then B component was added to A component and stirring continued to cool to room temperature to obtain a hair shampoo It was.

Example 14 Hair rinse [component A] part polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[Component B] Extract solution of Part Production Example 1 5.0
1,3-butylene glycol 5.0
Purified water Amounts of 100 parts in total A and B components were each heated to 80 ° C and dissolved uniformly, then B component was added to A component and stirring continued to cool to room temperature to obtain hair rinse It was.

Test Example 1 Anti-degranulation inhibitory test The anti-inflammatory effect of the apricot extract according to the present invention was evaluated by a cell anti-degranulation inhibitor test. Here, when skin cells are affected by external factors (ultraviolet rays or chemical substances), antigens are generated, and these antigens are degranulated (such as histamine, serotonin and eosinophil migration factor) of anti-basophils and mast cells. It is known to be involved in (release). Since this degranulation is also known to be involved in the development of skin inflammation and allergies, the anti-inflammatory effect was evaluated in the present invention by an anti-basophil degranulation inhibition test.

<Experiment method>
Rat basophil leukemia cells (RBL-2H3) were suspended in Eagle's minimum essential medium containing 10% NCS, seeded at 1 × 10 ^{5 in} 96-well plates, and cultured at 37 ° C. for 24 hours. Release confluent cells (117 mM NaCl, 5.4 mM KCl, 2.0 mM CaCl, 0.8 mM MgSO, 5.6 mM)
D-glucose, 25 mM HEPES (2- [4- (2-hydroxyethyl) -1-piperazinyl] ethanesulfonic acid), 1 mg / mL
After washing with 200 μL / well of BSA / pH 7.7], a sample solution was prepared by adding the extract solution of Production Example 1 to the releasing buffer. This sample solution is added to each well and further 100 μL of 200 μg / mL compound 48/80 (compound 48/80) / releasing buffer is added to induce degranulation and incubated at 37 ° C. for 1 hour. did. Here, a sample solution having a concentration prepared so that the final concentration (concentration as a solution) of the extract solution of Production Example 1 with respect to the total amount thereof was 2.0% was used. For comparison, two test sections to which a release buffer containing 30% 1,3-butylene glycol aqueous solution was added instead of the extract solution of Production Example 1 were provided, and one test section (blank) was provided. Only the releasing buffer was added to the other test group (control), and 100 μL of the same compound 48/80 / releasing buffer solution for degranulation as above was added and incubated at 37 ° C. for 1 hour. Furthermore, a strawberry tea extract (extract prepared as a solution with a final concentration of 5%) known to have a degranulation inhibitory effect was used as a positive control. After inducing degranulation, 50 μL of cell supernatant was dispensed into another 96-well microplate in order to measure the enzyme activity of β-hexosaminidase released to the outside of the cell. Measurement of β-hexosaminidase activity was performed as follows. That is, 5 μm p-nitrophenyl-2-acetamide-2-deoxy-β-glucopyranoside (p-Nitrophenyl-2-acetamide-2-deoxy-β-D-glucopyranoside) was used as a substrate in 50 μL of each cell supernatant taken on a separate plate. ) Was added and allowed to react for 30 minutes in a 37 ° C. CO ₂ incubator. Thereafter, 100 μL of 0.2 M glycine buffer (pH 10.7) was added to stop the reaction, the absorbance at 415 nm was measured with an absorbance plate reader (BIO RAD, Model 680), and β-hexosaminidase activity was measured. It was used as an index.

The results of Test Example 1 are shown in FIG. As shown in FIG. 1, it was revealed that the extract solution according to Production Example 1 of the present invention markedly suppresses degranulation of antibasophils. In addition, the strawberry tea extract, which is a positive control, showed the same effect, confirming that this test system was performed normally.

Test Example 2 DPPH radical scavenging test <Experimental method>
DPPH solution was prepared by dissolving 2.4 parts of DPPH in 20 parts of ethanol and adding 20 parts of purified water thereto. To 24 parts of this DPPH solution, 19.2 parts of 18 v / v% ethanol solution and 4.8 parts of 2M acetic acid-sodium acetate buffer (pH 5.5) were added to prepare a DPPH-added solution. In addition, in order to subtract the effect of the color of the extract itself on the test, a 50 v / v% ethanol solution was used instead of the DPPH solution, and a solution obtained by mixing an 18 v / v% ethanol solution and a 2M acetic acid-sodium acetate buffer solution was used. A control solution was used. Next, the extract solution of Production Example 1 was diluted with purified water to prepare a sample solution. Here, the sample solution has two concentrations prepared so that the final concentration (concentration as a solution) of the extract solution of Production Example 1 is 2.0% and 5.0% with respect to the total amount, respectively. It was used. The sample solution and DPPH added solution or control solution were mixed at a ratio of 1: 3, allowed to stand at room temperature for 10 minutes, and the absorbance at 550 nm when each test solution was mixed with DPPH added solution. The difference in absorbance at 550 nm when this was mixed with the control solution was measured to confirm the residual amount of DPPH radicals. At the same time, as a control, instead of the extract solution of Production Example 1, a 30% 1,3-butylene glycol aqueous solution was used to perform the same operation as above, and each sample for the DPPH radical residual rate (100) obtained here The relative value of DPPH radical residual rate at the time of addition was determined. Moreover, in order to confirm whether the test system is functioning normally, the same test was also performed when water-soluble vitamin E (final concentration 25 μM) was added as a positive control instead of the sample solution.

The results of Test Example 2 are shown in FIG. As shown in FIG. 2, it was revealed that the extract solution of Production Example 1 of the present invention has a significantly superior DPPH radical scavenging effect depending on the concentration. Moreover, since water-soluble vitamin E which is a positive control also showed a DPPH radical scavenging effect, it was confirmed that this test system was performed normally.

Test Example 3 SOD-like activity effect test <Experimental method>
0.2 M Tris-HCl buffer solution 50 μL, 1 mM ethylenediaminetetraacetic acid (EDTA) disodium solution 20 μL, 0.75 mM nitroblue tetrazolium chloride (NBT) solution 10 μL, 1 mM xanthine solution 20 μL, 0.06 U / mL xanthine oxidase solution 50 μL, production A sample solution was prepared by mixing 50 μL of the extract solution of Example 1. Here, the sample solution was prepared such that the final concentration (concentration as a solution) of the extract solution of Production Example 1 was 0.5%, 1.0%, and 2.0% with respect to the total amount thereof. Three concentrations were used. This sample solution was incubated at 37 ° C. for 3 minutes to generate superoxide. Then, the absorbance at 560 nm was measured for the amount of formazan produced by reducing NBT with superoxide. In addition, as a control, the same operation was performed on a solution prepared using a 30% 1,3-butylene glycol aqueous solution instead of the extract solution of Production Example 1, and the value obtained here was set to 100. The relative value of the amount of formazan at the time of addition was determined.

The results of Test Example 3 are shown in FIG. As shown in FIG. 3, the extract solution of Production Example 1 of the present invention was found to exhibit a significantly superior SOD-like activity, that is, an active oxygen scavenging action in a concentration-dependent manner.

Test Example 4 Recovery test for rough skin (1)
<Test Method> A test part (test area) of 1 cm square was provided on the inner side of each forearm for 5 subjects (men and women aged 20 to 60 years), washed, and allowed to rest for 15 minutes in a constant temperature and humidity room. As an initial value, the stratum corneum water content of the test part is measured by a stratum corneum water content measuring device (SKICON-200, manufactured by IBS), and the erythema amount is measured by an erythema amount measuring device (Megazameter Mexameter (registered trademark) MX18, Courage + Khazaka). Manufactured). After the measurement, a 5 w / w% sodium dodecyl sulfate (SDS) aqueous solution was subjected to an occlusion patch on the test site for 1 hour. After completion of the occlusion patch, the test part was washed with water and allowed to rest for 15 minutes in a constant temperature and humidity room, and then the stratum corneum water content and erythema amount of the test part immediately after the patch were measured with the above-described measuring apparatus. Then, twice a day, a lotion having the composition shown in Table 1 blended with the apricot extract of the present invention (sample of the present invention) was applied, and rested in a constant temperature and humidity room for 15 minutes on the fourth day of application. After that, the stratum corneum water content and the erythema amount were measured again with the measuring device, and the average value of five subjects was calculated. As a control, the same operation as described above was performed using purified water instead of the extract solution of Production Example 1. In addition, a control group not subjected to SDS treatment was provided, and also in this control group, the stratum corneum water content and erythema amount were measured in parallel. The stratum corneum moisture content and erythema amount were calculated. The stratum corneum moisture content was calculated as the stratum corneum moisture recovery rate according to the following formula 1.
Formula 1: stratum corneum moisture recovery rate (%) = (W ₄ −W ₁ / W ₀ −W ₁ ) × 100
(W ₀ : initial value of stratum corneum water content, W ₁ : stratum corneum water content immediately after patch, W ₄ : stratum corneum water content after 4 days)

The composition of the sample of the present invention used in Test Example 4 is as shown in Table 1 below.
[Table 1]

The results of Test Example 4 are shown in Table 2.
[Table 2]

The numerical values shown in Table 2 are average values of five subjects. As shown in Table 2, it became clear that the apricot extract of Production Example 1 according to the present invention significantly improved and improved the stratum corneum moisture content which was reduced by the influence of SDS. Furthermore, it has been clarified that erythema produced under the influence of SDS is remarkably improved. As for erythema, it was confirmed by visual observation that the redness of the skin was significantly recovered.

Test Example 5 Primary skin irritation suppression test (2)
<Test method>
For 5 subjects (men and women aged 20-60 years), a test part is provided on the inner side of the upper arm, and after washing, the amount of erythema in the test part of each subject is measured as an initial value (Meggazameter Mexameter (registered trademark)) MX18, manufactured by Courage + Khazaka). Thereafter, an occlusion patch of an aqueous solution containing laureth 5 (10% Polyoxyethylene 5 lauryl ether) known as a surfactant and the extract solution of Production Example 1 (final concentration of 2% as a solution) was applied to the test part for 1 hour. After completion of the occlusion patch, the test part was washed with water, and after 3 hours, the amount of erythema in the test part of each subject was measured with the above measuring device, and the value obtained by subtracting the value immediately after the treatment from the initial value was 5 as the primary stimulus suppression amount. The average value of subjects was calculated. As a control, the same operation as described above was performed using purified water instead of the extract solution of Production Example 1.

The results of Test Example 5 are shown in Table 3.
[Table 3]

The numerical values shown in Table 3 are average values of five subjects. As shown in Table 3, the control sample produced erythema in the test area of the subject under the influence of Laureth 5, whereas the sample of the present invention formulated with the apricot extract of Production Example 1 according to the present invention It was clearly shown that erythema was remarkably prevented and improved. It was also confirmed by visual observation that the redness of the test part to which the sample of the present invention was applied was remarkably suppressed as compared with the test part to which the control was applied.

As described above, since the extract according to the present invention has remarkably superior anti-inflammatory and antioxidant effects, it only prevents and improves skin aging (wrinkles, tarmi, etc.), as well as improves firmness and gloss. In addition, it also has an effect of protecting the skin from oxidative damage and inflammation damage due to external factors (ultraviolet rays and chemical substances). For this reason, the apricot extract according to the present invention is extremely useful as a component for anti-aging cosmetics and cosmetics for preventing and improving UV damage. Furthermore, it is extremely useful as a compounding ingredient for whitening cosmetics that prevents and improves spots, buckwheat, liver spots and the like based on anti-inflammatory and antioxidant effects.

  In addition, the extract of apricot (Hong apricot) according to the present invention has an effect of suppressing primary skin irritation, and also has an effect of preventing and improving inflammation caused by primary skin irritation. Substance) and a cosmetic compounding ingredient that protects the skin from irritating substances contained in the cosmetic.

Claims (1)

Cosmetics containing an extract of Prunus armeniaca Linne var anus Maximowicz or Prunus armeniaca Linne as an active ingredient.