JP6689024B2 - External skin preparation - Google Patents

Description

  The present invention relates to a cosmetic compounding ingredient having extracts of a plurality of plants as active ingredients and having excellent skin physiological activity and biosafety, and a skin external preparation containing such ingredients.

  Skin aging is an internal factor such as inactivation of cell proliferation and differentiation with aging, cell atrophy, decreased hormone secretion, and decreased amount of lipid components between stratum corneum, as well as sunlight (ultraviolet rays) and exhaust gas. It is a phenomenon caused by intricate entanglement with external factors such as cell / tissue damage due to active oxygen induced by chemical substances (nitrogen compounds, sulfur compounds, etc.) contained in etc., or inflammation. Due to the above-mentioned internal factors, for example, a decrease in the activity or proliferation of epidermal corneal cells, a decrease in components responsible for the barrier function of the stratum corneum such as ceramide and a natural moisturizing factor (NMF), and epidermal basal cell Inactivation and excessive sebum secretion occur. Further, due to the above-mentioned external factors, for example, the skin is oxidatively damaged, the biological components are deteriorated, and an antigen is generated in the skin, and the antigen causes inflammation of the skin and development of allergy. Then, as a morphological and physiological change of the skin due to these internal factors and external factors, symptoms related to skin aging such as rough skin, formation of acne, dry skin, wrinkles, and sagging appear.

  From the above, there has been a demand for an external skin ingredient having an effect of preventing and improving skin inflammation and acne, and an effect of improving the barrier function of the skin. Conventionally, various antioxidants (vitamin C, vitamin E) have been demanded. , Superoxide dismutase, etc.), anti-inflammatory agents (glycyrrhizic acid, etc.), and moisturizers (urea, etc.) have been proposed. However, these active ingredients have problems in terms of skin safety and effectiveness.

  In view of such problems of the prior art, the inventors of the present invention have conducted diligent research to find out new active ingredients derived from natural products from the viewpoint of skin safety. As a result, extracts of plants belonging to the genus Button, genus Rosaceae, and mixtures of extracts of plants belonging to the genus Lamiaceae, an excellent antioxidant action, anti-inflammatory action, It has a barrier function improving action, a skin turnover normalizing action, and a sebum secretion inhibiting action. Due to these actions, by blending the mixture, excellent skin physiological activity effects are exhibited, and skin safety is also excellent. It has been found that it is possible to provide a skin external preparation.

Conventionally, each of the plant extract belonging to the genus Button, the plant belonging to the genus Prunus and the plant belonging to the genus Lamiaceae has the skin physiological activity, for example, Patent Document 1 However, it has not been known that combining extracts of these plants creates a physiological physiological activity that could not be achieved by each extract.
JP-A-63-060935 Japanese Patent Laid-Open No. 01-090131 JP 06-279256 JP-A-58-172321 Japanese Patent Laid-Open No. 11-106336 JP 64-003126

  The present invention is an external preparation for skin containing an extract of a plant belonging to the genus Button of the family Botanaceae, an extract of a plant belonging to the genus Rosaceae and a plant belonging to the genus Lamiaceae.

  The present invention is an external preparation for skin containing an extract of a plant belonging to the genus Button of the family Botanaceae, an extract of a plant belonging to the genus Rosaceae, and an extract of a plant belonging to the Lamiaceae perilla, wherein the plant extract Antioxidant action, anti-inflammatory action, skin turnover normalizing action, barrier function improving action and sebaceous secretion inhibitory action, which are caused by the mixture of substances, and rough skin (inflammation, dry skin, adpy dermatitis, etc.), Prevents and improves pimples, skin wrinkles, and tarmi, improves skin firmness and luster, and further improves spots, freckles, and damage to the skin (oxidative damage and inflammatory damage) due to external factors such as ultraviolet rays. It is possible to provide a skin external preparation that exhibits preventive and improving effects. Moreover, since the mixture of the plant extract according to the present invention also has an antiandrogen action, it is possible to provide a skin external preparation for preventing hair growth or hair loss.

  Hereinafter, preferred embodiments of the present invention will be described in detail. In the present specification, the term "cosmetics" is used in a broad sense to include not only so-called cosmetics but also quasi drugs.

  The present invention is an external preparation for skin containing an extract of a plant belonging to the genus Button in the family Botanaceae, an extract of a plant belonging to the genus Rosaceae, and an extract of a plant belonging to the Labiatae family Lamiaceae. The extraction material to be used may be any species as long as it is a plant belonging to the genus Button, genus Prunus, and a plant belonging to the Lamiaceae genus.

  The species of the plant of the genus Paeoniacea (Paeoniaceae) used in the present invention is not particularly limited, and examples thereof include peony (Paeonia lactiflora), peony pierced pea (Paeonia japonica), and peony peony (Paeonia). , Buttons (Paeonia suffruticosa) and the like. Further, any plant such as whole plants, leaves, flower parts, stems, seeds, fruits and roots may be used, but the use of whole plants or roots is preferred.

  The species of the plant of the genus Rosaceae (Prunus) used in the present invention is not particularly limited, and examples thereof include peach (Prunus persica), apricot (Prunus vulgaris), plum (Prunus mume), and plum ( Prunus salicina) and the like. Further, any of whole plants, leaves, flower parts, stems, seeds, fruits, roots and the like of the plant may be used, but the use of whole plants or seeds is preferable.

  The plant species of the Lamiaceae genus (Perilla) used in the present invention is not particularly limited, and examples thereof include perilla and perilla. In addition, the perilla generally includes, as a scientific name, those represented by Perilla frutescens var. Crispa or Perilla frutescens var. Britton. For example, perilla frutescens var. Variants or subspecies, or hybrids may be mentioned, but the present invention is not limited thereto. Further, any of whole plants, leaves, flower parts, stems, seeds, fruits, roots and the like of the plant may be used, but the use of whole plants or leaves is preferable.

  To prepare the extract, first, the use site of each plant is washed with water beforehand to remove foreign matter, if necessary, and then dried as it is or after it is finely chopped or crushed, and contacted with an extraction solvent. Extract. The extraction can be carried out by bringing it into contact with an extraction solvent according to a conventional method such as an immersion method, but it is also possible to use a supercritical extraction method other than the immersion method.

  As the extraction solvent, water; lower alcohols such as methanol, ethanol, propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin; ethyl acetate, butyl acetate, methyl propionate, etc. Esters; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene, chloroform and the like, which may be used alone or in admixture of two or more. Used.

  Among these extraction solvents, the mixture of the obtained extracts has an antioxidative action, an antiinflammatory action, a sebum secretion inhibiting action, and a barrier function improving action, and further has a wide range of application to cosmetics from the viewpoint of skin irritation. From the viewpoint that it is also possible, hydrophilic solvents such as water, lower alcohols or polyhydric alcohols are preferable in the present invention. Preferable examples of using this hydrophilic solvent include, for example, water, lower alcohols (particularly ethanol), or polyhydric alcohols (particularly 1,3-butylene glycol) used alone, or water and lower alcohols. The use of a mixed solvent with (particularly ethanol) or a mixed solvent with water and a polyhydric alcohol (particularly 1,3-butylene glycol, glycerin) and the like can be mentioned. Among them, water alone or water and 1,3 -A mixed solvent of butylene glycol is particularly preferred.

  The mixing ratio in the case of using the mixed solvent is, for example, in the case of a mixed solvent of water and 1,3-butylene glycol, a volume ratio (the same applies hereinafter) of 1: 1 to 20: 1 and a mixed solvent of water and ethanol. If so, it is preferably in the range of 1: 1 to 25: 1, and in the case of a mixed solvent of water and glycerin, the range is 1: 1 to 20: 1.

  Moreover, the weight ratio of the use part of each plant and the extraction solvent is preferably 1: 1 to 1:50, and more preferably 1: 2 to 1:30.

  When preparing the extract, the pH is not particularly limited, but it is generally preferably in the range of 3-9. In this sense, if necessary, the extraction solvent may be mixed with an alkaline modifier such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic modifier such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid, You may adjust so that it may become pH.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used, but for example, when water or 1,3-butylene glycol or a mixed liquid of water and 1,3-butylene glycol is used as the solvent. If so, the extraction temperature is preferably in the range of 0 ° C to 80 ° C, more preferably in the range of 0 ° C to 20 ° C, and the extraction time is preferably 1 to 168 hours (1 hour to 1 week). , And more preferably from 1 to 120 hours (1 hour to 5 days).

  In addition, prior to the extraction treatment of the present invention or in parallel with the extraction treatment, the extract may be subjected to a hydrolysis treatment, if necessary. This may improve the storage stability of the extract and the like, so that the extract as a cosmetic compounding agent may be used more effectively.

  When the extract is subjected to enzymatic hydrolysis treatment, as the enzyme, actinase, papain, proteolytic enzymes such as pepsin, starch degrading enzymes such as glucoamylase, α-amylase, β-amylase, cellulase, hemicellulase, pectinase and the like. Although one or more kinds selected from any enzyme group such as fibrinolytic enzyme may be used, one kind or two or more kinds of enzymes respectively selected from those enzyme groups should be used in combination. Is more preferable.

  The amount of the enzyme added is, for example, preferably in the range of 0.01 to 10% by weight, and more preferably in the range of 0.1 to 2.0% by weight, based on the solid content of the use part of the plant. Is.

  The extract prepared as described above may be mixed and generally adjusted to pH 3 to 8 and then used as it is as a compounding agent for an external preparation for the skin. It may be used as the concentration of. Further, the extract may be a dried product by a conventional method such as a spray drying method.

  Examples of the external skin preparation (cosmetic, quasi drug, external drug) containing the mixture of the plant extract of the present invention include emulsion, cream, lotion, essence, pack, lipstick, foundation, liquid foundation, makeup press powder. Examples include, but are not limited to, blusher, white powder, face wash, body shampoo, hair shampoo, cleaning cosmetics such as soap, hair restorer, and bath agent.

  The amount of each plant extract of the present invention, when incorporated into a skin care external preparation for skin care, is generally 0.00001 to 5.0% by weight, preferably 0.0001 to 5.0% by weight as the solid content of each extract. It is in the range of 1.0% by weight. When blended in a skin external preparation for hair, the solid content of each extract is generally 0.00001 to 5.0% by weight (solid content% by weight, the same applies hereinafter), and preferably , 0.0001 to 3.0% by weight.

  In the external preparation for skin of the present invention, in addition to a mixture of plant extracts as essential components, as long as it is a skin external preparation for skin care, for example, an oily component, a surfactant (synthetic system, natural product system), a moisturizer, A thickener, an emulsifier or an emulsification aid, an antiseptic / bactericidal agent, a powder component, an ultraviolet absorber, an antioxidant, a dye, a fragrance, and other physiologically active components can be appropriately blended as necessary. In addition, if it is a skin external preparation for hair care, other active ingredients (hair matrix activator, anti-androgen, blood circulation promoter, sebum secretion inhibitor, anti-inflammatory agent, hair protectant, hair cycle growth maintenance Agents and the like) may be combined and blended, and by this, a synergistic hair growth effect and hair loss prevention effect can be expected. Further, as long as the effectiveness and characteristics of the mixture of the plant extract of the present invention are not impaired, it may be combined with other physiologically active ingredients in cosmetics.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadow foam oil, shea butter, tea tree oil, avocado oil, Plant-derived oils and fats such as macadamia nut oil and plant-derived squalane; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, butyl oleate 2-ethylhexyl glycerides, synthetic esters and synthetic triglycerides such as higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the like.

  As the surfactant, for example, polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, poly Nonionic surfactants such as oxyethylene sorbitol fatty acid ester; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, Anions such as polyoxyethylene alkyl ether phosphate, α-sulfonated fatty acid alkyl ester salt, and polyoxyethylene alkylphenyl ether phosphate Surfactants; quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzyl ammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N, Cationic surfactants such as N-dialkylmorpholinium salt and polyethylene polyamine fatty acid amide salt; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene Amphoteric surfactants such as ammoniocarboxybetaine and N-acylamidopropyl-N ', N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine can be used.

  As the emulsifier or emulsification aid, stevia derivatives such as enzyme-treated stevia, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacterium fermented rice, lactic acid bacterium fermented germinated rice, lactic acid bacterium fermented cereals (wheat, beans, millet, etc.), etc. It can also be blended.

  Moisturizers include, for example, glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, and sugars such as trehalose and mucopolysaccharides (for example, hyaluronic acid). Acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (Hakuo) Examples include extracts, various amino acids and their derivatives.

  Examples of the thickener include brown algae such as alginic acid, agar, carrageenan, and fucoidan, components derived from green algae or red algae; silane root (white and white) extract; polysaccharides such as pectin, locust bean gum, aloe polysaccharide; xanthan gum, Gums such as tragacanth gum and guar gum; cellulose derivatives such as carboxymethyl cellulose and hydroxyethyl cellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymers; hyaluronic acid and its derivatives; poly Examples thereof include glutamic acid and its derivatives.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate and butyl paraoxybenzoate; phenoxyethanol, dichlorophen, hexachlorophene, chlorhexidine hydrochloride, benza chloride. There are ruconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodinylurea), propanediol, 1,2-pentanediol, various essential oils, bark dry distillation products and the like.

  As the powder component, for example, sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic acid anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose. System powder, grains (rice, wheat, corn, millet, etc.) powder, beans (soybean, adzuki beans, etc.) powder, etc.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, and 2-hydroxy-4. -Methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (eg, vitamin E nicotinate, vitamin E linoleate, etc.), murasakikib extract, peony extract, silane root (white and white). ) There are extracts, etc.

  Whitening agents include t-cycloamino acid derivatives, kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4 -Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), 4- (4-hydroxyphenyl) -4-butanol)), hydroxybenzoic acid and its derivatives, vitamin E And derivatives thereof, α-hydroxy acid, and AMP (adenosine monophosphate, adenosine monophosphate), and these may be blended alone or in combination.

  Examples of the kojic acid derivatives include kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid esters such as kojic acid dibutyrate, kojic acid ethers, and kojic acid saccharides such as kojic acid glucoside. However, examples of the ascorbic acid derivative include L-ascorbic acid-2-phosphate sodium salt, L-ascorbic acid-2-phosphate magnesium salt, L-ascorbic acid-2-sulfate sodium salt, and L-ascorbic acid-2. -Ascorbic acid ester salts such as magnesium sulfate and the like, ascorbic acid sugar derivatives such as L-ascorbic acid-2-glucoside and L-ascorbic acid-5-glucoside, and the 6-position acylated products of these ascorbic acid sugar derivatives (acyl group: Hexanoyl group, octanoy Group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitic acid ester, L-ascorbic acid tetralauric acid ester and other L-ascorbic acid tetrafatty acid esters, 3-O-ethylascorbic acid, L-ascorbic acid-2 -Sodium phosphate-6-O-palmitate, glyceryl ascorbic acid or an acylated derivative thereof, glyceryl ascorbic acid and other ascorbic acid glycerin derivatives, and hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside), α -Arbutin (hydroquinone-α-D-glucopyranoside) and the like are, as tranexamic acid derivatives, tranexamic acid esters (for example, tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetixate). Ester or its salt), an amide of tranexamic acid (for example, tranexamic acid methylamide), and the like, and examples of the resorcinol derivative include 4-n-butylresorcinol, 4-isoamylresorcinol, and 2,5-dihydroxybenzoic acid. Examples of the acid derivative include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid and 2-hydroxy-5-propionyloxybenzoic acid, and examples of the nicotinic acid derivative include nicotinic acid amide and nicotinic acid. Examples of α-hydroxy acid such as benzyl include lactic acid, malic acid, succinic acid, citric acid and α-hydroxyoctanoic acid.

  Minoxidil, cyproterone acetate, pentadecanoic acid glyceride, 6-benzylaminopurine (cytopurine), adenosine, trans-3,4'-dimethyl-3-hydroxyflavanone are components that can be expected to have a synergistic effect of hair growth effect and hair loss prevention effect. (T-flavanone), assembly extract, hinokitiol, photosensitizer, pantothenic acid and its derivatives, Maimai flower extract, gentian extract, chamomile extract, vitamin E and its derivatives, nicotinic acid derivatives (nicotinic acid amide, etc.), carpronium chloride, female Hormones (ethinyl estradiol, estrone, etc.), ginkgo extract, clove extract, eelgrass extract, black soybean extract, salicylic acid, potassium glycyrrhizinate (liquorice extract), hinokitiol, benzalkonium chloride, iso Propylmethylphenol, 1-menthol, pyridoxine hydrochloride (vitamin E6), thioxolone, Dutch mustard extract, camphor, salicylic acid, resorcin, bis-type alkaloids obtained from Tamasakitsuduraji, Mitsuishikonbu, erucic acid (cis-13-docosenoic acid), Examples include higher monoenoic acids such as gondoinic acid (cis-11-eicosenoic acid), amino acids, vitamins, and fucoidan.

  As the physiologically active ingredient, as a whitening ingredient, for example, placenta extract, sow acupuncture extract, Yukinoshita extract, rice bran extract or its hydrolyzate, white mustard extract or its hydrolyzate, white mustard ferment, lactic acid bacteria Fermented rice, Murasakikib extract, lotus seed extract or its hydrolyzate, lotus seed fermented product, ginseng extract, coix seed hydrolyzate, coix seed fermented product, royal jelly fermented product, fermented sake lees, Pandanus amarilifolius ( Pandanus amaryllifolius Roxb.) Extract, Arcangelicia flava Merrilli extract, chamomile extract, etc. are listed, and as an anti-aging component, coral grass extract, rice leaf extract or its hydrolyzate , Eggplant (water eggplant, long eggplant, Kamo eggplant, rice eggplant etc.) extract or its hydrolyzate, apricot fruit extract, Damask rose extract, Extracts of seaweeds such as caterpillar Ginseng, extracts of marine flowering plants such as eelgrass, fermented soymilk, jellyfish water, rice extract or its hydrolyzate, rice fermentation extract, germinated rice extract or its hydrolyzate, Fermented sprouted rice, black soybean extract or its hydrolyzate, linoleic acid and its derivatives or processed products (for example, liposomal linoleic acid, etc.), animal or fish-derived collagen and its derivatives, elastin and its derivatives, glycyrrhizic acid and its Derivatives (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, aloe extract , Mitsuishikonbu extract, loofah extract, Anemarrhena marina extract, Juazeiro (Ziz yphus joazeiro) extract etc.

  Next, the present invention will be described in more detail with reference to production examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and% means% by weight.

Production Example 1. Mixture solution of plant extract: Peony roots of the genus Botanaceae are chopped and dried, and 50 g of the dried product is immersed in 250 g of purified water, and then 250 g of 1,3-butylene glycol is added. After addition, extraction was performed at 4 ° C. Then, the extract was filtered to obtain 442 g of a brown transparent peony extract solution (solid content concentration 3.57%). Also, peach seeds (tonin) of the genus Prunus rosaceae were dried and pulverized, 50 g of the pulverized product was immersed in 250 g of purified water, and 250 g of 1,3-butylene glycol was added. After addition, extraction was performed at 4 ° C. Then, the extract was filtered to obtain 493 g of a light yellow transparent tonin extract solution (solid content concentration: 1.74%). Further, perilla leaves of the Labiatae family Lamiaceae were dried, 25 g of the dried product was immersed in 250 g of purified water, and 250 g of 1,3-butylene glycol was added. After addition, extraction was performed at 4 ° C. Then, the extract was filtered to obtain 448 g of a brown transparent perilla extract solution (solid content concentration 1.02%). After mixing the above-mentioned three kinds of plant extracts, the insoluble matter was filtered to obtain 1230 g of a brown transparent extract mixed solution (solid content concentration 1.54%).

Prescription example 1. Lotion [A component] part Olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[Component B] Part Mixed solution of Production Example 1 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide Suitable amount Purified water Total amount of 100 parts [C component]
Fragrance: Appropriate amount Each of the components A and B was heated to 80 ° C. or higher, then the component B was added to the component A, the mixture was stirred, and further homogenized with a hyscotron (5000 rpm) for 2 minutes. After cooling this to 50 ° C., component C was added and mixed with stirring, and further cooled to 30 ° C. or lower to obtain a lotion.

Prescription example 2. Emulsion [A component] part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[Component B] Part Mixed solution of Production Example 1 3.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water 100 parts [C component]
Fragrances Appropriate amount The above components A and B were each heated to 80 ° C. or higher, and then mixed with stirring. After cooling this to 50 ° C., the component C was added and further stirred and mixed to obtain an emulsion.

Prescription example 3. Emulsion Emulsion in the same manner as in Prescription Example 2 except that 2.0 parts of L-ascorbic acid-2-glucoside and 2.0 parts of potassium hydroxide were used in the B component of Prescription Example 2 instead of 2.0 parts of tranexamic acid. Got

Prescription example 4. Emulsion An emulsion was prepared in the same manner as in Formulation Example 2 except that 2.0 parts of L-ascorbic acid-2-glucoside and 3.0 parts of arbutin were used instead of 2.0 parts of L-ascorbic acid-2-glucoside in the B component of Formulation Example 2. Obtained.

Prescription example 5. Emulsion [A component] part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[Component B] Part Mixed solution of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Arbutin 3.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water 100 parts

Prescription example 6. Essence [ingredient] part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Mixture solution of Production Example 1 5.0
Citric acid 0.3
Sodium citrate 0.6
Purified water Total amount is 100 parts After dissolving hyaluronic acid in purified water, the remaining raw materials were sequentially added and dissolved with stirring to obtain a transparent essence.

Example 7. Liquid foundation [A component] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[Component B] Part Mixed solution of Production Example 1 5.0
Carboxymethyl cellulose sodium 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Total amount is 100 parts [C component] part Titanium oxide 8.0
Talc 4.0
Coloring Pigments Appropriate amounts The above-mentioned components A and B were each heated and then mixed and stirred. This was reheated, the above-mentioned C component was added, and the mixture was poured into a mold and stirred until it reached room temperature to obtain a liquid foundation.

Prescription example 8. Hair tonic for hair growth [ingredient] part l-menthol 0.8
Preparation Example 1 mixture solution 2.0
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Ethanol 20.0
Amount of purified water to be 100 parts The above ingredients were thoroughly mixed with stirring to obtain a hair restorer.

  The skin physiological activity of the plant extract of the present invention was evaluated by the following test methods. In addition, in this test example, evaluation was performed using a sample solution prepared so as to contain an equal amount of the extract of each plant, but the present invention is not limited to this.

Test Example 1. DPPH radical scavenging test 2.4 parts of DPPH was dissolved in 20 parts of ethanol, and 20 parts of purified water was added thereto to prepare a DPPH solution. To 24 parts of this DPPH solution, 19.2 parts of 18 v / v% ethanol solution and 4.8 parts of 2M acetic acid-sodium acetate buffer (pH 5.5) were added to prepare a DPPH addition solution. In addition, in order to subtract the influence of the color tone of the extract itself on the test, a 50 v / v% ethanol solution was used instead of the DPPH solution, and a solution obtained by mixing an 18 v / v% ethanol solution and a 2M acetic acid-sodium acetate buffer solution was used. It was used as a control solution. Next, the mixture solution of Production Example 1 was diluted with purified water to prepare a sample solution. Here, three kinds of sample solutions were used so that the final concentration of the mixture solution (concentration as a solution) was 0.1%, 0.2%, and 0.6%, respectively. . The sample solution and the DPPH-added solution or the control solution were mixed at a ratio of 1: 3, and after standing at room temperature for 10 minutes, the absorbance at 550 nm when each test solution was mixed with the DPPH-added solution, and each test solution The difference with the absorbance at 550 nm in the case of mixing with the control solution was measured to confirm the residual amount of DPPH radicals. At the same time, as a control (Control), a 50% 1,3-butylene glycol aqueous solution was used in place of the mixture solution of Production Example 1, and the same operation was performed as described above to add each sample to the DPPH radical residual rate obtained here. The relative value of the DPPH radical residual rate at that time was obtained. In addition, in order to confirm whether the test system is functioning normally, the same test was carried out also in the case where Trolox (final concentration 5 μM) which is water-soluble vitamin E was added as a positive control instead of the sample solution. .

The results of Test Example 1 are shown in Table 1.
[Table 1]

  As shown in Table 1, the mixture solution according to the present invention was shown to have a markedly superior DPPH radical scavenging action in a concentration-dependent manner. Similarly, the positive control water-soluble vitamin E (Trolox) also showed a DPPH radical scavenging action, and it was also confirmed that this test system was performed normally.

Test example 2. SOD (superoxide dismutase) -like activity effect test 0.2 M Tris-HCl buffer 50 μL, 1 mM ethylenediaminetetraacetic acid (EDTA) disodium solution 20 μL, 0.75 mM nitroblue tetrazolium chloride (NBT) solution 10 μL, 1 mM xanthine solution 20 μL, 0 A sample solution was prepared by mixing 50 μL of the 0.06 U / mL xanthine oxidase solution and 50 μL of the extract solution of Production Example 1. Here, the sample solution used was prepared such that the final concentration (concentration as a solution) of the mixture solution was 0.1%, 0.2%, and 0.6%. This sample solution was incubated at 37 ° C. for 3 minutes to generate superoxide. Then, the amount of formazan produced by reducing NBT with superoxide was measured by measuring the absorbance at 570 nm. Further, as a control (Control), the same operation was performed on a solution prepared by using a 30% 1,3-butylene glycol aqueous solution instead of the mixture solution of Production Example 1, and the value obtained here was set to 100, The relative value of the amount of formazan at the time of adding each sample was determined. As a positive control, the same operation was performed for SOD (10 unit).

The results of Test Example 2 are shown in Table 2.
[Table 2]

  As shown in Table 2, the mixture solution according to the present invention was shown to have a markedly superior SOD-like activity in a concentration-dependent manner. Similarly, the positive control also showed SOD-like activity, confirming that the test system was normally performed.

Test example 3. Prostaglandin E ₂ (PGE ₂ ) production inhibitory effect test The anti-inflammatory effect of the mixture solution according to the present invention was evaluated by a PEG ₂ production inhibitory test. Here, PGE2 is an inflammatory chemical mediator that causes skin inflammation, and is a substance secreted in skin cells damaged by external factors such as ultraviolet rays and chemical substances. Therefore, in the present invention, the anti-inflammatory effect was evaluated by this PGE ₂ production inhibitory effect test.

<Experimental method>
Usagi cornea-derived cells (SIRC) were suspended in 10% FBS-containing minimal essential medium of Eagle, seeded in 96-well plates at 5.0 × 10 ³ each, and cultured at 37 ° C. for 4 days, then from the bottom of the incubator. The mixture was irradiated with an ultraviolet B wave of 100 mJ / cm ² , and the mixture solution (sample solution) of Production Example 1 was added to the culture solution. Here, the sample solution was added so that the final concentration of the solution was 0.1% with respect to the total amount of the culture solution. After the addition, the cells were cultured for 2 days, and the amount of PGE ₂ secreted in the culture supernatant was measured using a PGE ₂ measurement kit (manufactured by Cayman Chemical). Further, the amount of PEG ₂ was measured by the same operation as above, in which a 50% 1,3-butylene glycol aqueous solution was added instead of the sample solution as a control. Further, the amount of PGE ₂ when 10 μM of indomethacin was used as a positive control instead of the sample solution was also measured in the same manner. Further, a 50% 1,3-butylene glycol aqueous solution was added instead of the sample solution, and after culturing for 24 hours, the amount of PGE ₂ was measured also in a control group not irradiated with ultraviolet rays.

The results of Test Example 3 are shown in Table 3.
[Table 3]

As shown in Table 3, the mixture solution according to the present invention was shown to have a markedly excellent PGE ₂ inhibitory effect. Further, it was shown that indomethacin, which is a positive control, also had the same effect, and it was also confirmed that this test system was normally performed.

Test example 4. Ceramide synthase (β-glucocerebrosidase) activity enhancement effect test Human epidermal cells PHK16-0b were placed in a 96-well microplate containing MCDB153 medium (manufactured by SIGMA) containing HKGS (manufactured by Kurabo Industries) and 1 × 10 ⁴ cells / After seeding in a hole and precultured at 37 ° C. under 5.0% CO ₂ for 3 days, the mixture solution (sample solution) of Production Example 1 was concentrated to 0.1% and 0.5% (solution to medium). Final concentration) was added to the medium, and the cells were further cultured for 4 days under the same conditions. Next, the medium was removed, and 20 μL of 1 mM Phenylmethylsulfonylfluoride (PMSF), 1% Triton-X-containing PBS (−) solution was added, and the mixture was left standing at room temperature for 5 minutes to crush the cells to obtain a crude enzyme solution. . 20 μL of 1 mM 4-methylbelliferyl-β-Glucopyranoside, 10 mM sodium taurocholate, 0.1% Triton-Xin 0.1M citrate phosphate buffer (pH 5.6) was added and reacted at 37 ° C. for 1 hour. After completion of the reaction, the reaction was stopped by adding 200 μL of 0.2 M carbonic acid bicarbonic acid buffer (pH 10.5). Then, the fluorescence intensity at Ex355 / Em460 of the reaction solution was measured and taken as the β-glucocerebrosidase activity value. Further, the same operation as above was carried out for the case where the sample without addition of PBS (-) in place of the sample solution was added (control), and each sample was compared with the β-glucocerebrosidase activity value (100) obtained here. The relative value of β-glucocerebrosidase activity in the addition group was determined, and this value was defined as the β-glucocerebrosidase activity rate (%). Moreover, in order to confirm whether the test system is functioning normally, the same test was performed in the case where 0.005% galactocerebroside (GalCer) was added as a positive control instead of the sample solution.

The results of Test Example 4 are shown in Table 1.
[Table 4]

  As shown in Table 4, the mixture solution according to the present invention was shown to have a markedly excellent ceramide synthase (β-glucocerebrosidase) activity-enhancing action in a concentration-dependent manner. It was also shown that CalCer, which is a positive control, also has a β-glucocerebrosidase activity-enhancing activity, and it was also confirmed that this test system was performed normally.

Test example 5. Lipase activity inhibitory effect test The following measurements were performed using Lipase Kit S (DS Pharma Biomedical). Two glass test tubes were prepared for each sample, one for the sample (A) and the other for the blind test (B). In both A and B test tubes, 40 μL of a sample solution containing 1 mL of a color developing solution (containing 5,5′-dithiobis (2-nitrobenzoic acid)) and the mixture solution of Production Example 1 (the final concentration of the mixture solution as a solution is 1.0%) and 40 μL of lipase (manufactured by Sigma) and mixed, and then 100 μL of substrate solution (containing dimercaprol tributyrate (BALB) and sodium dodecyl sulfate (SDS)) in test tube A only. Immediately after mixing, the mixture was incubated at 30 ± 1 ° C. for exactly 30 minutes. Immediately after completion of the incubation, 2 mL of the reaction stop solution was added to the test tube A. Then, 2 mL of the reaction stop solution was added to the test tube B, 100 μL of the substrate solution was added only to the test tube B, and the mixture was mixed again. The absorbance of the sample (A) and the blind test (B) were measured at a wavelength of 415 nm. The value obtained by subtracting the absorbance at the wavelength of 415 nm of the blind test (B) from the absorbance at the wavelength of 415 nm of the sample (A) was defined as the lipase activity. Further, the same test was performed using purified water instead of each sample solution, and the relative value of the lipase activity of each sample solution was calculated when the lipase activity obtained here was 100.

The results of Test Example 5 are shown in Table 5.
[Table 5]

  As shown in Table 5, the mixture solution according to the present invention showed a remarkably excellent inhibitory effect on lipase activity.

Test example 6. Androgen binding inhibitory effect test Mouse-derived breast cancer cell SC-3 showing androgen-dependent proliferation was seeded at 5 × 10 ³ cells / hole on a 96-well microplate containing 2% FBS (charcoal dextrin-treated) Eagle's minimum essential medium. After pre-culturing at 37 ° C. under 5.0% CO ₂ for 1 day, the mixture solution (sample solution) of Production Example 1 was adjusted to 0.6% concentration (final concentration as a solution) in HMB medium. (HAM medium: Eagle medium = 1: 1) was used to replace the medium of the cell culture system. Further, in combination with the sample, a group to which 10 nM DHT (dihydrotestosterone [highly active androgen]) was added and a group not to be added were set. The cells were cultured under the same conditions for 3 days. Then, the medium was removed, 0.03% MTT was added, and the mixture was kept at 37 ° C. for 1 hour, and then the formed formazan was extracted with isopropanol, and a microplate reader (Model 680, manufactured by Bio-Rad) was used. The MTT value was measured at a wavelength of 570-630 nm. The same operation as above is performed in the case of no sample added (Control) in which PBS (-) is added instead of the sample solution, and the MTT value at the time of each sample addition to the MTT value obtained in the Control of the DHT addition group, And the relative value of the MTT value obtained in the control of the DHT-free group was determined and defined as the proliferation rate (%) of SC-3 cells.

The results of Test Example 6 are shown in Table 6.
[Table 6]

  As shown in Table 6, in the control, the growth of SC-3 cells was promoted by DHT, whereas the mixture solution of the present invention markedly suppressed the growth of SC-3 cells, that is, significantly. It has been shown to have excellent anti-androgenic activity.

Test Example 7. Sebum secretion inhibitory effect test A healthy subject was washed with a commercially available face cleanser, and both cheeks were wiped with 75% ethanol, and then the room temperature was kept at 20 ° C and humidity was 50 to 60%. received. After resting for 30 minutes, the amount of sebum on both cheeks was measured using Sebumeter (registered trademark) SM815 (manufactured by Courage + Khazaka (Germany)), and this was used as the initial value of each subject. A lotion (A) prepared by adding the mixture solution (sample solution) of Production Example 1 to a final concentration of 2% as a solution and a lotion (B) "Control" containing purified water instead of the sample were prepared. Was applied to the left cheek and B to the right cheek twice a day in the morning and night (at the time of application, the subject was applied in a so-called blind state so that the subject could not know which one was the sample lotion). After 2 weeks and 4 weeks, the amount of sebum in each test part (both cheeks) was measured by the same method as the measurement of the initial value.

The results of Test Example 7 are shown in Table 7.
[Table 7]

  As shown in Table 7, the mixture solution according to the present invention was shown to have a significantly superior sebum secretion inhibitory action.

Test Example 8. Evaluation Test of Kallikrein-7 Activity Enhancement Effect In this Test Example 8, the activity of a chymotrypsin serine protease (kallikrein-7) that promotes exfoliation of the stratum corneum in normal skin and affects the normalization of turnover of the stratum corneum. The enhancement was evaluated.
[Test method]
Normal human epidermal keratinocytes were prepared at 2 × 10 ⁵ cells / mL with HuMedia-KB2 [registered trademark] (produced by Kurabo Co., Ltd.) containing a growth additive, 100 μL was seeded on a 96-well microplate, and 5% carbonic acid was added. Incubation was carried out at 37 ° C under gas and saturated steam. After 24 hours, a culture solution containing the mixture solution (sample solution) according to Production Example 1 of the present invention was additionally added and cultured. Here, the sample solution was prepared so that the final concentration was 0.5% and 1.0% as a solution with respect to the total amount of the culture solution. Further, a test section to which a culture solution containing 50% 1,3-butylene glycol was additionally added so that the final concentration was 1.0% was set as a control. After 72 hours, the cell culture supernatant of each test section was removed, and the cells were disrupted using 20 μL of Tris-hydrochloric acid buffer solution (pH 8.0) containing 0.14 M sodium chloride and 0.1% Tween 20 to give a crude sample. The enzyme solution was used. To the crude enzyme solution, 30 μL of 100 mM Tris-HCl buffer (pH 7.8) containing 0.1% Tween 20 as a buffer and 50 μL of the above buffer containing 100 mM Suc-Leu-Leu-Val-Tyr-MCA as a substrate were added. The mixture was reacted at 37 ° C. for 1 hour while stirring. After completion of the reaction, the fluorescence intensity (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems), and this was designated as kallikrein-7 activity. Further, another plate on which the same culturing operation was performed was prepared, and after removing the culture supernatant, 100 μL of Hoechst 33342 solution was added and reacted at 37 ° C. for 1 hour. After the reaction was completed, the fluorescence intensity (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) was measured using a fluorescence plate reader, and the amount of DNA was determined. The obtained value was used to calculate the kallikrein-7 activity per DNA.

The results of Test Example 8 are shown in Table 8.
[Table 8]

  As shown in Table 8, the extract according to Production Example 1 of the present invention was shown to enhance the significantly excellent activity of kallikrein-7 in a concentration-dependent manner.

As described above, the mixture of the plant extract according to the present invention has antioxidative action (DPPH radical scavenging action, SOD-like action action), anti-inflammatory action (PGE ₂ inhibitory action), ceramide synthase (β-glucocerebrosidase). ) It has an activity enhancing action, a skin turnover normalizing action, a lipase activity inhibiting action, an antiandrogen action, and a sebum secretion inhibiting action. Thus, according to the present invention, due to their synergistic effect, rough skin and atopic dermatitis, pimples, and wrinkles on the skin, improve the tarmi, improve the firmness and gloss of the skin, and further, stains and freckles. It is possible to provide an external preparation for skin that exhibits improvement and prevention and improvement effects on skin damage (oxidative damage and inflammatory damage) due to external factors such as ultraviolet rays. Furthermore, it is also possible to improve the blockage of pores and the like caused by excessive sebum secretion, and the mixture of the plant extract according to the present invention has an antiandrogen action and a sebaceous secretion inhibitory action. According to this, an external preparation for skin for preventing hair growth or hair loss can also be provided.

Claims (1)

Peony (Paeoniaceae) Peony root extract belonging to Paeonia, Paeonia root extract, Rosaceae (Prunus) peach seed extract, and Lamiaceae (Perilla) belonging to Lamiaceae (Perilla) A skin external preparation containing an extract of a perilla leaf to which it belongs.