CN114344229A - Natural plant bacteriostatic no-clean disinfectant and preparation method and application thereof - Google Patents

Natural plant bacteriostatic no-clean disinfectant and preparation method and application thereof Download PDF

Info

Publication number
CN114344229A
CN114344229A CN202111561883.XA CN202111561883A CN114344229A CN 114344229 A CN114344229 A CN 114344229A CN 202111561883 A CN202111561883 A CN 202111561883A CN 114344229 A CN114344229 A CN 114344229A
Authority
CN
China
Prior art keywords
percent
extract
natural plant
disinfectant
carbomer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202111561883.XA
Other languages
Chinese (zh)
Inventor
郑丛龙
许恒毅
郭四姣
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Anson Biotechnology Co ltd
Original Assignee
Anson Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anson Biotechnology Co ltd filed Critical Anson Biotechnology Co ltd
Priority to CN202111561883.XA priority Critical patent/CN114344229A/en
Publication of CN114344229A publication Critical patent/CN114344229A/en
Pending legal-status Critical Current

Links

Images

Landscapes

  • Cosmetics (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a natural plant bacteriostatic no-clean disinfectant, a preparation method and an application thereof, wherein the natural plant bacteriostatic no-clean disinfectant comprises the following raw materials in percentage by weight: 50 to 70 percent of ethanol, 0.001 to 0.05 percent of triclosan, 0.1 to 1 percent of pomegranate peel extract, 0.1 to 3 percent of honeysuckle extract, 0.1 to 1 percent of wild chrysanthemum extract, 3 to 20 percent of aloe extract, 0.01 to 1 percent of pH regulator, 0.05 to 2 percent of carbomer, 0.5 to 10 percent of humectant and the balance of water. The washing-free disinfectant takes ethanol, dichlorophenoxy chlorophenol, pomegranate rind extract, honeysuckle extract, wild chrysanthemum extract and aloe extract as main antibacterial components, and has the advantages of good stability, broad-spectrum sterilization, long-acting bacteriostasis, difficult generation of drug resistance and the like.

Description

Natural plant bacteriostatic no-clean disinfectant and preparation method and application thereof
Technical Field
The invention relates to the technical field of sanitary cleaning products, in particular to a natural plant bacteriostatic no-clean disinfectant and a preparation method and application thereof.
Background
Microbial infection is an important cause for human diseases, and hand contact is a main path for pathogen transmission, so hand cleaning and disinfection are important. Studies have shown that washing hands with clean water alone is not sufficient to remove bacteria from the hands, whereas washing hands with a hand sanitizer or soap is effective in removing bacteria from the hands. The year 2008 is the international environmental sanitation year established by the major congress of the united nations, the initiative of the personal public and private organization for soap washing (PPPHW) to initiate the activity of washing hands on the global day is promoted, and the activity of washing hands with soap is called for 10, 15 days per year from 2008 in all countries around the world. The scientific hand washing method requires that the soap or the liquid soap is used for washing hands before and after meals, before meals, after sneezing or coughing, before eyes, mouths and noses are touched and the like.
The existing hand sanitizer with the disinfection function has the problems of single component, unsatisfactory disinfection effect, obvious pungent smell in the use process, harm to the health of a human body even caused by volatile components, and the like. Therefore, the development of safe and effective washing-free disinfectant has important significance.
Disclosure of Invention
Therefore, there is a need for a natural plant bacteriostatic no-clean disinfectant, which has the advantages of high safety, good stability, broad-spectrum sterilization, good bacteriostatic effect and the like.
The invention provides a natural plant bacteriostatic no-clean disinfectant, which comprises the following raw materials in percentage by weight:
50 to 70 percent of ethanol, 0.001 to 0.05 percent of triclosan, 0.1 to 1 percent of pomegranate peel extract, 0.1 to 3 percent of honeysuckle extract, 0.1 to 1 percent of wild chrysanthemum extract, 3 to 20 percent of aloe extract, 0.01 to 1 percent of pH regulator, 0.05 to 2 percent of carbomer, 0.5 to 10 percent of humectant and the balance of water.
In some embodiments, the natural plant bacteriostatic no-clean disinfectant comprises the following raw material components in percentage by weight: 55 to 70 percent of ethanol, 0.005 to 0.04 percent of dichlorophenoxy chlorophenol, 0.1 to 1 percent of pomegranate peel extract, 0.2 to 2 percent of honeysuckle extract, 0.1 to 1 percent of wild chrysanthemum extract, 5 to 15 percent of aloe extract, 0.01 to 1 percent of pH regulator, 0.05 to 2 percent of carbomer, 0.5 to 8 percent of humectant and the balance of water.
In some embodiments, the composition comprises the following raw material components in percentage by weight:
55 to 65 percent of ethanol, 0.005 to 0.03 percent of dichlorophenoxy chlorophenol, 0.1 to 1 percent of pomegranate peel extract, 0.2 to 1 percent of honeysuckle extract, 0.1 to 1 percent of wild chrysanthemum extract, 5 to 15 percent of aloe extract, 0.01 to 1 percent of pH regulator, 0.05 to 2 percent of carbomer, 0.5 to 5 percent of humectant and the balance of water.
In some of these embodiments, the carbomer is carbomer 2020.
In some of these embodiments, the pH adjusting agent is selected from at least one of triethanolamine, ethylenediamine, and ethanolamine.
In some of these embodiments, the pH adjusting agent is triethanolamine.
In some of these embodiments, the humectant is glycerin and/or propylene glycol.
In another aspect of the invention, a preparation method of the natural plant bacteriostatic no-clean disinfectant is provided, which comprises the following steps:
providing raw materials according to the component proportion;
and uniformly mixing the raw materials.
In some embodiments, the step of uniformly mixing the raw materials comprises:
s1, uniformly mixing the carbomer, the pH regulator and a proper amount of water, and swelling to obtain carbomer gel mother liquor;
s2, uniformly mixing the ethanol, the triclosan, the pomegranate bark extract, the honeysuckle extract, the wild chrysanthemum flower extract, the aloe extract and the humectant to obtain a premixed solution.
S3, mixing the carbomer gel mother solution with the premix, and fixing the volume to obtain the natural plant bacteriostatic no-wash disinfectant.
In another aspect of the invention, the natural plant bacteriostatic no-clean disinfectant is applied to inhibiting staphylococcus aureus, escherichia coli, pseudomonas aeruginosa and/or klebsiella pneumoniae.
The invention has the beneficial effects that:
(1) the natural plant bacteriostatic no-clean disinfectant is prepared by combining ethanol and dichlorophenoxy chlorophenol with a plurality of traditional Chinese medicinal materials such as a pomegranate rind extract, a honeysuckle extract, a wild chrysanthemum extract and an aloe extract, and the components play a synergistic complementation and promotion role, so that the bactericidal effect can be improved, the disinfection and sterilization effects of some components cannot be influenced by chemical reaction among the components, and the natural plant bacteriostatic no-clean disinfectant has the advantages of broad-spectrum disinfection, strong bactericidal capacity, long bacteriostatic time, good stability, low toxicity, safety to people and animals and the like, and is simple in preparation method and low in cost.
(2) The ethanol used in the natural plant bacteriostatic no-wash disinfectant can be used as a solvent to dissolve the effective components in the triclosan and the natural plant extract except for being a good bactericidal disinfectant, and has good dissolving effect; the carbomer is dissolved to form elastic gel with a space net structure, the dichlorophenoxy chlorophenol, the traditional Chinese medicine extract and other components can be uniformly dispersed in the carbomer, and the carbomer can continuously exert the disinfection and bacteriostasis effects after being used; meanwhile, through the matching use of various disinfection and sterilization components, the dosage of ethanol and triclosan can be reduced while the disinfection and sterilization effects are ensured, the irritation and damage to the hand skin are reduced, the skin can be protected and the skin is prevented from being dried by further adding a humectant, and the no-clean disinfectant disclosed by the invention is in a gel state, so that the hand feel is fresh and not sticky after the no-clean disinfectant is used.
(3) The natural plant bacteriostatic no-clean disinfectant has the advantages of easily available raw materials, simple and convenient preparation method and good product stability.
Drawings
FIG. 1 is a photograph showing the comparison of the bactericidal effects of the bacteriostatic no-clean disinfectant prepared according to example 1 and a pruela hand-cleaning disinfectant;
FIG. 2 is a photograph showing the comparison of the bacteriostatic effects of the bacteriostatic no-wash disinfectant prepared from natural plants in example 1 and the bacteriostatic effect of a pruela hand-cleaning disinfectant;
FIG. 3 is a photograph comparing the bactericidal effects of the bacteriostatic no-clean disinfectant prepared in example 2 with those of a medical alcohol disinfectant;
FIG. 4 is a photograph comparing the bacteriostatic effects of the natural plant bacteriostatic and wash-free disinfectant prepared in example 2 with those of a medical alcohol disinfectant.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments and comparative examples of the present invention are given in the accompanying drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
The invention provides a natural plant bacteriostatic no-clean disinfectant, which comprises the following raw materials in percentage by weight:
50 to 70 percent of ethanol, 0.001 to 0.05 percent of triclosan, 0.1 to 1 percent of pomegranate peel extract, 0.1 to 3 percent of honeysuckle extract, 0.1 to 1 percent of wild chrysanthemum extract, 3 to 20 percent of aloe extract, 0.01 to 1 percent of pH regulator, 0.05 to 2 percent of carbomer, 0.5 to 10 percent of humectant and the balance of water.
The stable no-clean disinfectant is prepared by matching and combining ethanol, triclosan and natural plant extracts (pomegranate bark extract, honeysuckle extract, wild chrysanthemum extract and aloe extract) with a sterilization effect, adding carbomer, a pH regulator, a humectant and the like, strictly controlling the dosage of each component, and has the advantages of strong sterilization, long-acting bacteriostasis, difficult generation of drug resistance and good virus killing effect due to the synergistic effect of each component.
Dichlorophenoxy chlorophenol is a broad-spectrum antibacterial agent, has broad killing and inhibiting effects on infection or pathogenic gram-positive bacteria, fungi, yeast and viruses (such as hepatitis A, hepatitis B, rabies virus and HIV), and has the characteristics of high efficiency, safety, no irritation to skin and high stability.
The tannin (such as ellagic acid) contained in pericarpium Granati extract can coagulate protoplasm of microorganism and multiple enzymes, has inhibitory effect on various bacteria and fungi, exhibits broad-spectrum antibacterial property, and has drug-resistant bacteria resisting effect.
The honeysuckle extract is extracted from natural plant honeysuckle, the main component of the honeysuckle extract is chlorogenic acid, and the honeysuckle extract has strong antibacterial power for streptococcus, staphylococcus, typhoid bacillus, dysentery bacillus, large intestine, pseudomonas aeruginosa, pneumococcus, pertussis bacillus, meningococcus and the like.
The flos Chrysanthemi Indici extract is a mixture rich in flavone extracted from capitula of Compositae flos Chrysanthemi Indici, and has broad antibacterial spectrum, and its decoction and ethanol extract have inhibitory effect on Staphylococcus aureus, Bacillus tuberculosis, diphtheria bacillus, Escherichia coli, hemolytic streptococcus, various dysentery bacillus, Pseudomonas aeruginosa, Bacillus proteus, Vibrio cholerae and Bacillus calmette-guerin in vitro test. Has obvious inhibition effect on common bacteria causing respiratory diseases and enterobacteria, and also has inhibition effect on influenza viruses.
The aloe extract contains abundant anthrone compounds, which have antibacterial, antiinflammatory, toxic materials clearing away, wound healing promoting, and moisture keeping effects.
In some embodiments, the natural plant bacteriostatic no-clean disinfectant comprises the following raw material components in percentage by weight: 55 to 70 percent of ethanol, 0.005 to 0.04 percent of dichlorophenoxy chlorophenol, 0.1 to 1 percent of pomegranate peel extract, 0.2 to 2 percent of honeysuckle extract, 0.1 to 1 percent of wild chrysanthemum extract, 5 to 15 percent of aloe extract, 0.01 to 1 percent of pH regulator, 0.05 to 2 percent of carbomer, 0.5 to 8 percent of humectant and the balance of water.
In some embodiments, the natural plant bacteriostatic no-clean disinfectant comprises the following raw material components in percentage by weight:
55 to 65 percent of ethanol, 0.005 to 0.03 percent of dichlorophenoxy chlorophenol, 0.1 to 1 percent of pomegranate peel extract, 0.2 to 1 percent of honeysuckle extract, 0.1 to 1 percent of wild chrysanthemum extract, 5 to 15 percent of aloe extract, 0.01 to 1 percent of pH regulator, 0.05 to 2 percent of carbomer, 0.5 to 5 percent of humectant and the balance of water.
In some embodiments, the aloe extract is aloe vera extract. It will be appreciated that the aloe extract may be in the form of a powder, a paste or a liquid, preferably an aloe extract in the form of a liquid.
In some embodiments, the weight ratio of carbomer to pH modifying agent is (1-2): 1.
In some embodiments, the carbomer is one or a combination of carbomer 2020, carbomer 980, carbomer 940, carbomer 941, and the like.
After the carbomer is neutralized by the pH regulator, molecular chains are dispersed and stretched due to the mutual repulsion action of negative charges, the carbomer is in a sticky gel shape, the dichlorophenoxychlorophenol, the traditional Chinese medicine extract and other components can be uniformly dispersed in the carbomer, and the carbomer can play a role in sterilization and disinfection for a long time and a long time.
Preferably, the carbomer is carbomer 2020.
Carbomer 2020 has the advantages of easy wetting, good thickening effect, easy wetting and swelling, convenient use and good compatibility with other components in the system.
In some embodiments, the pH adjusting agent is selected from at least one of triethanolamine, ethylenediamine, and ethanolamine.
Therefore, the pH value of the mixed system can be well adjusted by adding a proper amount of organic amine pH regulator.
Preferably, the pH adjusting agent is triethanolamine.
The pH value of a mixed system is adjusted by using triethanolamine with alkalescence, and the triethanolamine is neutralized with carboxyl of carbomer to form a stable high molecular structure, so that the effects of thickening and moisturizing can be achieved.
In some embodiments, the humectant is glycerin and/or propylene glycol.
In another aspect of the invention, a preparation method of a natural plant bacteriostatic no-clean disinfectant is provided, which is used for preparing the natural plant bacteriostatic no-clean disinfectant, and the method comprises the following steps:
providing raw materials according to the component proportion;
and uniformly mixing the raw materials.
In some embodiments, the step of mixing the raw materials uniformly is as follows:
s1, uniformly mixing carbomer, a pH regulator and a proper amount of water, and swelling to obtain carbomer gel mother liquor;
s2, mixing ethanol, triclosan, pericarpium Granati extract, flos Lonicerae extract, flos Chrysanthemi Indici extract, Aloe extract and humectant uniformly to obtain premixed solution.
S3, mixing the carbomer gel mother solution and the premix solution uniformly, and adding water to a constant volume to obtain the natural plant bacteriostatic no-wash disinfectant.
It is understood that the steps S1 and S2 are not strictly sequential, and may be performed simultaneously or alternatively.
Specifically, step S1 is as follows:
dissolving carbomer in water to obtain 2-5 wt% carbomer solution, adding the pH regulator according to the mass ratio of carbomer to pH regulator (1-2) to 1, uniformly mixing, wrapping a beaker with tinfoil paper, and swelling for 10-12 h at room temperature in shade to obtain carbomer gel mother solution.
Step S2 is as follows:
dissolving dichlorophenoxy chlorophenol in an appropriate amount of ethanol to obtain an dichlorophenoxy chlorophenol ethanol solution;
dissolving the pomegranate bark extract, the honeysuckle extract and the wild chrysanthemum extract in a proper amount of ethanol to obtain a first plant extract mixed solution;
uniformly mixing carbomer gel mother liquor, aloe extract and humectant, adding triclosan ethanol solution and the mixed solution of the first plant extract, adding water to a constant volume, uniformly mixing, and standing for 8-24 h to obtain the natural plant antibacterial no-wash disinfectant.
In another aspect of the invention, the natural plant bacteriostatic no-clean disinfectant is applied to inhibiting staphylococcus aureus, escherichia coli, pseudomonas aeruginosa and/or klebsiella pneumoniae. The natural plant bacteriostatic no-wash disinfectant has good killing and inhibiting effects on staphylococcus aureus, escherichia coli, pseudomonas aeruginosa and/or klebsiella pneumoniae.
The washing-free disinfectant disclosed by the invention has the beneficial effects that the sterilization and bacteriostasis effects are cooperatively exerted by the combined use of various sterilization active ingredients of ethanol, dichlorophenoxy chlorophenol and natural plant extracts (pomegranate bark extract, honeysuckle extract, wild chrysanthemum extract and aloe extract), so that the broad spectrum of sterilization is ensured, and the generation of bacterial drug resistance is effectively avoided; it can kill over 99.99% of hand bacteria in 1min, and can effectively kill antibiotic-resistant bacteria.
Meanwhile, the volatility of ethanol in the no-clean disinfectant enables hands to quickly recover dry and comfortable after cleaning, triclosan and natural plant bacteriostatic components remained on the hands can resist bacteria for a long time, and the addition of moisturizing components such as glycerin and the like ensures that the skin of the hands is moisturized for a long time, so that the no-clean disinfectant has good sterilizing, bacteriostatic and moisturizing effects.
In addition, the invention adopts ethanol, triclosan and natural plant extracts as bactericidal active ingredients, avoids the use of chemical disinfection ingredients such as benzalkonium chloride, chlorhexidine gluconate and the like, and has the characteristic of ecological environmental protection.
The following are specific examples.
The reagents used in the embodiment of the invention are all common reagents and can be purchased from conventional reagent production and sale companies. Wherein, the pomegranate bark extract contains 28 to 50 percent of ellagic acid, the honeysuckle extract contains 3 to 8 percent of chlorogenic acid, the wild chrysanthemum extract contains 3 to 8 percent of linarin, and the aloe extract contains 20 to 38 percent of aloin.
Example 1
1. The preparation process of the carbomer gel comprises the following steps:
weighing 2g of carbomer 2020, dissolving in 100mL of deionized water, adding 1.15g of triethanolamine, uniformly mixing, and naturally swelling for 12h to obtain carbomer gel mother liquor.
2. The preparation process of the natural plant extract comprises the following steps:
mixing 0.1g pericarpium Granati extract (containing 40% ellagic acid), 0.4g flos Lonicerae extract (containing 5% chlorogenic acid), and 0.1g flos Chrysanthemi Indici extract (containing 5% linarin), dissolving in 9mL anhydrous ethanol, mixing, and standing for 6 hr.
3. The preparation process of the triclosan ethanol solution comprises the following steps:
0.01g of triclosan (purity 99%) is dissolved in 4mL of absolute ethanol and mixed well.
4. The preparation process of the natural plant bacteriostatic no-clean disinfectant comprises the following steps:
weighing 10g of carbomer gel mother liquor, adding 15mL of deionized water, 10mL of aloe extract (30% barbaloin) and 0.5g of glycerol, slowly stirring, uniformly mixing, then adding 8mL of pre-prepared clear plant extract ethanol solution, 4mL of dichlorophenoxy chlorophenol ethanol solution and 53mL of absolute ethanol, slowly stirring until the system is clear, diluting the deionized water to a constant volume of 100mL, and swelling in a cool place at room temperature for 12 hours to obtain the natural plant antibacterial no-clean disinfectant.
5. The natural plant bacteriostatic no-clean disinfectant has the functions of sterilization and bacteriostasis:
(1) and (3) bacterial culture: ten clinically separated drug-resistant strains are obtained from hospitals and are respectively Escherichia coli DZ100, Escherichia coli DP59, Escherichia coli DP86, Pseudomonas aeruginosa CE2, Pseudomonas aeruginosa CZ3, Staphylococcus aureus CP121, methicillin-resistant Staphylococcus aureus 14121, methicillin-resistant Staphylococcus aureus 8212, methicillin-resistant Staphylococcus aureus 5641 and Klebsiella pneumoniae AY215, single colonies on an LB solid culture medium are respectively picked up and inoculated on an LB liquid culture medium according to the proportion of 1 percent, and the mixture is placed at 37 ℃ and cultured for 12 hours at 180rpm by a shaking table.
(2) The experiment for comparing the sterilization effects of the natural plant bacteriostatic no-wash disinfectant and the prulai hand-cleaning disinfectant comprises the following steps: diluting fresh cultured bacteria liquid to 10% with sterile peptone water6CFU/mL, respectively weighing 0.5g of natural plant bacteriostatic and wash-free disinfectant in a group a of 5mL sterile centrifuge tubes, 0.5g of Pruieley hand-cleaning disinfectant in a group b of 5mL sterile centrifuge tubes, 0.5g of sterile deionized water in a group c of 5mL sterile centrifuge tubes, respectively taking 100 mu L of ten diluted bacteria liquid in three groups of centrifuge tubes, uniformly mixing for 1min, adding 3mL of sterile deionized water in the centrifuge tubes, uniformly mixing, respectively taking 100 mu L of ab group sample, coating the 100 mu L of ab group sample on an LB solid culture medium, diluting the c group sample with peptone water for ten times, taking 100 mu L of diluent, coating the LB solid culture medium, inversely culturing for 24h at 37 ℃, observing results, and paralleling each group for three times.
(3) The antibacterial effect comparison experiment of the natural plant antibacterial no-clean disinfectant and the Puruilai hand-cleaning disinfectant comprises the following steps: diluting fresh cultured bacteria liquid to 10% with sterile peptone water3CFU/mL～104CFU/mL, respectively weighing 0.5g of natural plant antibacterial no-clean disinfectant in a d group of LB solid culture medium, 0.5g of Puruilai hand-cleaning disinfectant in an e group of LB solid culture medium, 0.5g of sterile deionized water in a f group of LB solid culture medium, uniformly coating the natural plant antibacterial no-clean disinfectant in the f group of LB solid culture medium by using a coating rod, placing the natural plant antibacterial no-clean disinfectant in a sterile super clean bench for air drying, respectively taking 100 mu L of diluted bacterial liquid to coat the bacterial liquid in the three groups of LB solid culture media, placing the bacterial liquid in the sterile super clean bench for air drying, and after inverted culture at 37 ℃ for 24 hours, observing results, wherein each group is paralleled for three times.
As a result: the test results are shown in fig. 1 and fig. 2, and the bactericidal effect and the bacteriostatic effect of the natural plant bacteriostatic no-clean disinfectant of example 1 are compared with those of a pruela hand-cleaning disinfectant. In the figure 1, three groups of a, b and c are respectively escherichia coli DZ100, escherichia coli DP59, escherichia coli DP86, pseudomonas aeruginosa CE2, pseudomonas aeruginosa CZ3, staphylococcus aureus CP121, methicillin-resistant staphylococcus aureus 14121, methicillin-resistant staphylococcus aureus 8212, methicillin-resistant staphylococcus aureus 5641 and klebsiella pneumoniae AY215 from left to right. In fig. 2, three groups d, e and f are respectively escherichia coli DZ100, escherichia coli DP59, escherichia coli DP86, pseudomonas aeruginosa CE2, pseudomonas aeruginosa CZ3, staphylococcus aureus CP121, methicillin-resistant staphylococcus aureus 14121, methicillin-resistant staphylococcus aureus 8212, methicillin-resistant staphylococcus aureus 5641 and klebsiella pneumoniae AY215 from left to right.
Experiments show that the natural plant bacteriostatic and wash-free disinfectant provided by the embodiment 1 can effectively kill escherichia coli DZ100, escherichia coli DP59, escherichia coli DP86, pseudomonas aeruginosa CE2, pseudomonas aeruginosa CZ3, staphylococcus aureus CP121, methicillin-resistant staphylococcus aureus 14121, methicillin-resistant staphylococcus aureus 8212, methicillin-resistant staphylococcus aureus 5641 and klebsiella pneumoniae AY215 within 1min, and the bactericidal effect of the natural plant bacteriostatic and wash-free disinfectant is equivalent to that of a proclaia hand-cleaning disinfectant; the natural plant bacteriostatic washing-free disinfectant in the embodiment 1 can effectively inhibit the growth of bacteria, and the bacteriostatic effect of the natural plant bacteriostatic washing-free disinfectant is superior to that of a pureland hand-cleaning disinfectant.
Example 2
1. The preparation process of the carbomer gel comprises the following steps:
weighing 2g of carbomer 2020, dissolving in 100mL of deionized water, adding 1g of triethanolamine, uniformly mixing, and naturally swelling for 12h to obtain 2% carbomer gel mother liquor.
2. The preparation process of the natural plant extract comprises the following steps:
weighing 0.4g of pericarpium Granati extract (containing 40% ellagic acid), 0.7g of flos Lonicerae extract (containing 5% chlorogenic acid), and 0.3g of flos Chrysanthemi Indici extract (containing 5% linarin), mixing, dissolving in 18ml of anhydrous ethanol, mixing, and standing for 6 hr.
3. Preparing a natural plant bacteriostatic no-clean disinfectant:
weighing 20g of 2% carbomer 2020 gel, adding 30mL of deionized water, 0.02g of triclosan (the purity is 99%), 20mL of aloe extract (30% barbaloin) and 1g of glycerin, slowly stirring, uniformly mixing, then adding 16mL of the clarified plant extract ethanol solution pre-dissolved in the step 2 and 113mL of absolute ethanol, slowly stirring until the system is clarified, diluting the deionized water to a constant volume of 200mL, and swelling for 12 hours in a cool place at room temperature to obtain the natural plant antibacterial no-clean disinfectant.
4. The natural plant bacteriostatic no-clean disinfectant has the bacteriostatic action:
(1) and (3) bacterial culture: single colonies of staphylococcus aureus CMCC 26003, escherichia coli CMCC 44102, pseudomonas aeruginosa CMCC 10104, bacillus subtilis CMCC 63501 and candida albicans CMCC 98001 on an LB solid culture medium are picked, inoculated into an LB liquid culture medium according to the proportion of 1 percent, and cultured for 12 hours in a shaking way at 37 ℃ and 180 rpm.
(2) The experiment for comparing the sterilization effects of the natural plant bacteriostatic no-wash disinfectant and the prulai hand-cleaning disinfectant comprises the following steps: diluting fresh cultured bacteria liquid to 10% with sterile peptone water6CFU/mL, respectively weighing 0.5g of natural plant bacteriostatic wash-free disinfectant in g groups of 5mL sterile centrifuge tubes, 0.5g of Prelet hand-cleaning disinfectant in h groups of 5mL sterile centrifuge tubes, 0.5g of sterile deionized water in i groups of 5mL sterile centrifuge tubes, respectively taking 100 mu L of five diluted bacterial solutions in three groups of centrifuge tubes, uniformly mixing for 1min, adding 3mL of sterile deionized water in the centrifuge tubes, uniformly mixing, respectively taking 100 mu L of g and h groups of samples, coating the g and h groups of samples on an LB solid culture medium, diluting the i groups of samples with peptone water for ten times, taking 100 mu L of the diluted solution, coating the diluted solution on the LB solid culture medium, inversely culturing for 24h at 37 ℃, observing the result, and enabling each group to be parallel for three times.
(3) The antibacterial effect comparison experiment of the natural plant antibacterial no-clean disinfectant and the Puruilai hand-cleaning disinfectant comprises the following steps: diluting fresh cultured bacteria liquid to 10% with sterile peptone water3～104CFU/mL, respectively weighing 0.5g of natural plant antibacterial no-clean disinfectant in g group LB solid culture medium, 0.5g of Puruilai hand-cleaning disinfectant in h group LB solid culture medium, 0.5g of sterile deionized water in i group LB solid culture medium, uniformly coating the natural plant antibacterial no-clean disinfectant in a coating rod, placing the group of natural plant antibacterial no-clean disinfectant in a sterile super clean bench for air drying, respectively taking 100 mu L of five diluted bacterial liquids to coat the group of natural plant antibacterial no-clean disinfectant in three groups of LB solid culture media, placing the group of natural plant antibacterial no-clean disinfectant in the sterile super clean bench for air drying, and after inverted culture at 37 ℃ for 24 hours, observing results, wherein each group is paralleled for three times.
As a result: the test results are shown in fig. 3 and 4, and the bactericidal effect and bacteriostatic effect of the natural plant bacteriostatic no-clean disinfectant of example 2 are compared with the bactericidal effect and bacteriostatic effect of the medical alcohol disinfectant. In FIG. 3, three groups g, h and i are respectively Escherichia coli DZ100, Escherichia coli DP59, Escherichia coli DP86, Pseudomonas aeruginosa CE2, Pseudomonas aeruginosa CZ3, Staphylococcus aureus CP121, methicillin-resistant Staphylococcus aureus 14121, methicillin-resistant Staphylococcus aureus 8212, methicillin-resistant Staphylococcus aureus 5641 and Klebsiella pneumoniae AY215 from left to right; in FIG. 4, three groups of j, k and m are respectively Escherichia coli DZ100, Escherichia coli DP59, Escherichia coli DP86, Pseudomonas aeruginosa CE2, Pseudomonas aeruginosa CZ3, Staphylococcus aureus CP121, methicillin-resistant Staphylococcus aureus 14121, methicillin-resistant Staphylococcus aureus 8212, methicillin-resistant Staphylococcus aureus 5641 and Klebsiella pneumoniae AY215 from left to right.
Experiments show that the natural plant bacteriostatic and wash-free disinfectant provided by the embodiment 2 can effectively kill staphylococcus aureus CMCC 26003, escherichia coli CMCC 44102, pseudomonas aeruginosa CMCC 10104, bacillus subtilis CMCC 63501 and candida albicans CMCC 98001 within 1min, and the bactericidal effect of the natural plant bacteriostatic and wash-free disinfectant is obviously superior to that of a pureland hand-cleaning disinfectant; the natural plant bacteriostatic washing-free disinfectant in the embodiment 2 can effectively inhibit the growth of bacteria, and the bacteriostatic effect of the natural plant bacteriostatic washing-free disinfectant is superior to that of a pureland hand-cleaning disinfectant.
Example 3
The preparation of example 3 was substantially the same as in example 1, except that the amount of ethanol used was different, that is, the amount of anhydrous ethanol used in step 4 was 45 mL.
Through sterilization and bacteriostasis tests, the natural plant bacteriostatic no-wash disinfectant in the embodiment 3 has the same sterilization and bacteriostasis effects as those in the embodiment 1, the sterilization effect is also equivalent to that of a pureland hand-cleaning disinfectant, and the bacteriostasis effect is superior to that of the pureland hand-cleaning disinfectant.
It is worth noting that in the experiment, when the dosage of the ethanol is lower than 50% of the total amount of the raw materials, the sterilization effect of the no-clean disinfectant is obviously reduced.
Example 4
Example 4 was prepared essentially the same as example 2, except that step 3: example 4 triclosan was used in an amount of 0.04g in step 3.
The tests show that the bactericidal and bacteriostatic effects of the example 4 are equivalent to those of the example 2.
Example 5
Example 5 was prepared substantially identically to example 1, except that the same weight of propylene glycol was used in place of the glycerin.
The experiment proves that the sterilization and bacteriostasis effects of the embodiment 5 are equivalent to those of the embodiment 1.
Comparative example 1
Comparative example 1 was prepared substantially the same as example 1, except that: comparative example 1 no triclosan was added.
Through tests, the sterilizing effect of the disinfectant of the comparative example 1 is poorer than that of the propyzamide disinfectant, is slightly better than that of medical alcohol, and the bacteriostatic effect is poorer than that of the propyzamide disinfectant.
Comparative example 2
Comparative example 2 is substantially the same as example 1 except that: comparative example 2 no pomegranate extract, honeysuckle extract, wild chrysanthemum extract and aloe extract were added.
The disinfectant of comparative example 2 has a lower bactericidal and bacteriostatic effect than that of example 1, and the bactericidal effect is also lower than that of propyzamide.
Comparative example 3
Comparative example 3 is essentially the same as example 3, except that no carbomer and pH adjusting agent are added, i.e.:
(1) weighing 0.4g of pericarpium Granati extract (containing 40% ellagic acid), 0.7g of flos Lonicerae extract (containing 5% chlorogenic acid), and 0.3g of flos Chrysanthemi Indici extract (containing 5% linarin), mixing, dissolving in 18ml of anhydrous ethanol, mixing, and standing for 6 hr.
(2) Weighing 0.02g of triclosan (with the purity of 99%), 20mL of aloe extract (with the purity of 30% barbaloin) and 1g of glycerol, slowly stirring, uniformly mixing, then adding 16mL of clear plant extract ethanol solution pre-dissolved in the step (1) and 113mL of absolute ethanol, slowly stirring until the system is clear, diluting the volume of deionized water to 200mL, and swelling in a cool place at room temperature for 12 hours to obtain the no-clean disinfectant.
The sterilization and bacteriostasis tests are carried out according to the example 2, and the sterilization and bacteriostasis effect of the no-clean disinfectant of the comparative example 3 is obviously poorer than that of the example 1 and is slightly better than that of medical alcohol.
Stability test
After the natural plant bacteriostatic no-clean disinfectant disclosed by the embodiment 1-5 is taken and stored in a sealed manner at the temperature of 30 ℃ for 270 days, the disinfectant is clear, transparent and free from turbidity, and is used for carrying out sterilization and bacteriostatic tests on escherichia coli DZ100, escherichia coli DP59, escherichia coli DP86, pseudomonas aeruginosa CE2, pseudomonas aeruginosa CZ3, staphylococcus aureus CP121, methicillin-resistant staphylococcus aureus 14121, methicillin-resistant staphylococcus aureus 8212, methicillin-resistant staphylococcus aureus 5641 and klebsiella pneumoniae AY215, and the killing effect and the bacteriostatic effect are not obviously different from those before storage.
And (4) conclusion: the washing-free disinfectant disclosed by the embodiment of the invention has good stability, and keeps good sterilization, disinfection and bacteriostasis effects within 9 months.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. The natural plant bacteriostatic no-clean disinfectant is characterized by comprising the following raw materials in percentage by weight: 50 to 70 percent of ethanol, 0.001 to 0.05 percent of triclosan, 0.1 to 1 percent of pomegranate peel extract, 0.1 to 3 percent of honeysuckle extract, 0.1 to 1 percent of wild chrysanthemum extract, 3 to 20 percent of aloe extract, 0.01 to 1 percent of pH regulator, 0.05 to 2 percent of carbomer, 0.5 to 10 percent of humectant and the balance of water.
2. The natural plant bacteriostatic no-clean disinfectant as claimed in claim 1, which comprises the following raw material components by weight percent: 55 to 70 percent of ethanol, 0.005 to 0.04 percent of dichlorophenoxy chlorophenol, 0.1 to 1 percent of pomegranate peel extract, 0.2 to 2 percent of honeysuckle extract, 0.1 to 1 percent of wild chrysanthemum extract, 5 to 15 percent of aloe extract, 0.01 to 1 percent of pH regulator, 0.05 to 2 percent of carbomer, 0.5 to 8 percent of humectant and the balance of water.
3. The natural plant bacteriostatic no-clean disinfectant as claimed in claim 2, which comprises the following raw material components in percentage by weight: comprises the following raw material components: 55 to 65 percent of ethanol, 0.005 to 0.03 percent of dichlorophenoxy chlorophenol, 0.1 to 1 percent of pomegranate peel extract, 0.2 to 1 percent of honeysuckle extract, 0.1 to 1 percent of wild chrysanthemum extract, 5 to 15 percent of aloe extract, 0.01 to 1 percent of pH regulator, 0.05 to 2 percent of carbomer, 0.5 to 5 percent of humectant and the balance of water.
4. The natural plant bacteriostatic no-wash disinfectant as claimed in any one of claims 1 to 3, wherein the carbomer is carbomer 2020.
5. The natural plant bacteriostatic no-clean disinfectant as claimed in any one of claims 1 to 3, wherein the pH regulator is at least one selected from triethanolamine, ethylenediamine and ethanolamine.
6. The natural plant bacteriostatic no-clean disinfectant as claimed in any one of claims 1 to 3, wherein the pH regulator is triethanolamine.
7. The natural plant bacteriostatic no-clean disinfectant as claimed in claims 1-3, wherein the humectant is glycerol and/or propylene glycol.
8. A preparation method of the natural plant bacteriostatic no-clean disinfectant as claimed in any one of claims 1 to 7, characterized by comprising the following steps:
providing raw materials according to the component proportion;
and uniformly mixing the raw materials.
9. The method of claim 8, wherein the step of uniformly mixing the raw materials comprises:
s1, uniformly mixing the carbomer, the pH regulator and a proper amount of water, and swelling to obtain carbomer gel mother liquor;
s2, uniformly mixing the ethanol, the triclosan, the pomegranate bark extract, the honeysuckle extract, the wild chrysanthemum flower extract, the aloe extract and the humectant to obtain a premixed solution.
S3, mixing the carbomer gel mother solution with the premix, and fixing the volume to obtain the natural plant bacteriostatic no-wash disinfectant.
10. The natural plant bacteriostatic and no-clean disinfectant liquid as claimed in any one of claims 1 to 7 or prepared by the preparation method as claimed in claim 8 or 9, and application thereof in inhibiting staphylococcus aureus, escherichia coli, pseudomonas aeruginosa and/or klebsiella pneumoniae.
CN202111561883.XA 2021-12-17 2021-12-17 Natural plant bacteriostatic no-clean disinfectant and preparation method and application thereof Pending CN114344229A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111561883.XA CN114344229A (en) 2021-12-17 2021-12-17 Natural plant bacteriostatic no-clean disinfectant and preparation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111561883.XA CN114344229A (en) 2021-12-17 2021-12-17 Natural plant bacteriostatic no-clean disinfectant and preparation method and application thereof

Publications (1)

Publication Number Publication Date
CN114344229A true CN114344229A (en) 2022-04-15

Family

ID=81100461

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111561883.XA Pending CN114344229A (en) 2021-12-17 2021-12-17 Natural plant bacteriostatic no-clean disinfectant and preparation method and application thereof

Country Status (1)

Country Link
CN (1) CN114344229A (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102755275A (en) * 2012-07-24 2012-10-31 河南华美生物工程有限公司 Dry cleaning and disinfecting gelatin prepared by using plant extracts
JP2015143205A (en) * 2013-12-27 2015-08-06 共栄化学工業株式会社 External preparation for skin
CN106728630A (en) * 2016-12-22 2017-05-31 广州睿森生物科技有限公司 A kind of anti-inflammation composition and privates care product
CN109498732A (en) * 2018-11-26 2019-03-22 郑州吉尔康消毒制品有限公司 Hand-wrist bones gel and preparation method thereof
CN111184675A (en) * 2020-02-18 2020-05-22 广东省粤企产业创新研究院 No-clean disinfection composition, preparation method thereof and no-clean disinfection gel
CN113144058A (en) * 2021-03-03 2021-07-23 惠州市至上新材料有限公司 Skin-moistening refreshing wash-free disinfection gel and preparation method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102755275A (en) * 2012-07-24 2012-10-31 河南华美生物工程有限公司 Dry cleaning and disinfecting gelatin prepared by using plant extracts
JP2015143205A (en) * 2013-12-27 2015-08-06 共栄化学工業株式会社 External preparation for skin
CN106728630A (en) * 2016-12-22 2017-05-31 广州睿森生物科技有限公司 A kind of anti-inflammation composition and privates care product
CN109498732A (en) * 2018-11-26 2019-03-22 郑州吉尔康消毒制品有限公司 Hand-wrist bones gel and preparation method thereof
CN111184675A (en) * 2020-02-18 2020-05-22 广东省粤企产业创新研究院 No-clean disinfection composition, preparation method thereof and no-clean disinfection gel
CN113144058A (en) * 2021-03-03 2021-07-23 惠州市至上新材料有限公司 Skin-moistening refreshing wash-free disinfection gel and preparation method thereof

Similar Documents

Publication Publication Date Title
US20020068101A1 (en) Antimicrobial composition formulated with essential oils
CN111449093A (en) Natural essential oil composition with antibacterial and disinfecting effects and application thereof
CN101766552A (en) Washing-free antibacterial hand sanitizer and preparation method thereof
CN1232665A (en) Antimicrobial composition
CN107812137A (en) A kind of draft skin bacteriostatic gel of propolis-containing and preparation method thereof
CN109771315A (en) A kind of disposable hand-wrist bones gel of environment-friendly type and preparation method thereof
CN103977155A (en) Cleaning-free compound hand sanitizer and preparation method thereof
TW201216859A (en) Disinfectant and antiseptic formulation having reduced iodine content
CN110478293A (en) A kind of disposable disinfection hand cleanser and preparation method thereof
CN106309174A (en) Folium isatidis hand cleanser and preparation method thereof
CN111671709A (en) Tea tree essential oil and ethanol double-disinfection and washing-free hand sanitizer and preparation method thereof
CN111184675A (en) No-clean disinfection composition, preparation method thereof and no-clean disinfection gel
CN108684743A (en) A kind of sheep hurdle air sanitizer and preparation method thereof
CN111297730A (en) Long-acting harmless, washing-free and non-flammable macromolecular ethanol disinfectant
CN107737290A (en) A kind of Chinese medicine composition, pharmaceutical preparation and its application
KR101835098B1 (en) Hand wash compositions using natural extracts
CN110251580B (en) Composite antibacterial agent and preparation method and application thereof
CN107535540A (en) A kind of antiseptic and preparation method thereof
CN107568222A (en) A kind of compound glutaraldehyde Deciquam persistent disinfectant
CN104381326A (en) Anti-mold sterilization aromatic bead
CN1046621C (en) Complex iodine liquid disinfectant
CN114344229A (en) Natural plant bacteriostatic no-clean disinfectant and preparation method and application thereof
CN111067866A (en) Wash-free ethanol hand disinfection spray
CN104207971B (en) Medical skin-disinfection and -nursing hand-cleaning liquid and preparation method thereof
CN111840188A (en) Nursing type hand-washing-free disinfection gel and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20220415