JP2005022993A - Agent for promoting production of collagen and elastin and ageing-resistant cosmetic containing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an agent for promoting the production of collagen and elastin as the matrix components of corium to hold or improve the skin in young and tensive states, when applied to the skin, and to provide an ageing-resistant cosmetic which contains the agent and exhibits excellent effects for preventing the ageing of the skin and improving the chapping of the skin. <P>SOLUTION: This agent for promoting the production of collagen and elastin contains the extract of a plant belonging to Hippophae in Elaeagnaceae as an active ingredient. The agent for promoting the production of collagen and elastin contains the extract of a plant belonging to Hippophae rhamnoides L. as an active ingredient. The ageing-resistant cosmetic contains the agent for promoting the production of the collagen and elastin. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

【００６１】
表１に示すように、本発明のヒッポファエ属植物の抽出物は、ＡＰＭに匹敵する高いコラーゲン産生促進作用を有する。
【００６２】
試験例２．エラスチン産生促進作用
実施例１で得られた抽出物溶液を試料として用い、本発明のヒッポファエ属植物抽出物のエラスチン産生促進能を調べた。
【００６３】
［試験方法］
正常ヒト皮膚由来線維芽細胞（ＮＢ１ＲＧＢ）を、０．５％ＮＣＳ含有イーグルＭＥＭ培地を入れた９６穴マイクロプレートに１．０×１０^４個／穴になるように播種し、３７℃で２４時間培養した後、培地に試料（実施例１の抽出物溶液）を２．５又は５．０％の濃度（溶液として）となるように添加し、さらに７日間培養した。培養上清に分泌されたエラスチン量を抗ヒトエラスチン抗体を用いたＥＬＩＳＡ法で測定した。
【００６４】
［結果］
結果を表２に示した。
【表２】

【００６５】
表２に示すように、本発明のヒッポファエ属植物抽出物は、試料無添加に比べ顕著にエラスチン産生を促進した。
【００６６】
試験例３．皮膚刺激性
［試料］
（１）実施例１で得られた抽出物溶液を日局親水ワセリンに５％の濃度となるように練合したもの（本発明試料）
（２）日局親水ワセリン（対照）
【００６７】
［試験方法］
年齢２０〜５０歳の成人男子５名を被験者とし、各々の上腕部内側をエタノールで拭って皮脂を除去し、該部位に、フィンチャンバーのアルミ板に各試料をそれぞれ０．２ｇ宛塗布したものを貼付した。２４時間後にフィンチャンバーを除去し、皮膚刺激の程度をつぎに述べる方法並びに基準により判定した。
［判定］
パッチ除去後１時間後、２４時間後及び４８時間後に、貼付部位の紅斑及び浮腫の状況を、以下の「ドレイズ法による皮膚刺激性判定基準」に基づき目視判定し、被験者５名の平均値を求めた。

【００６８】
［結果］
結果を表３に示す。
【表３】

【００６９】
表３の結果から明らかな通り、ヒッポファエ属植物の抽出物は皮膚に対する刺激が殆どなく、生体安全性に極めてすぐれている。
【００７０】
試験例４．パネルテスト
本発明のコラーゲン及びエラスチン産生促進剤を含む老化防止化粧料について、パネルテストにより皮膚に対する効果を調べた。
［試料］
（１）処方例１のクリーム （本発明例）
（２）処方例２のクリーム （本発明例）
（３）処方例１に於いて、実施例１の抽出物溶液の代わりに精製水を用いて得られたクリーム（対照）
【００７１】
［試験方法］
無作為に抽出した年齢１８〜５０歳の女性４０名を被験者として２０名ずつ２つのグループに分け、一方のグループに処方例１のクリームと対照のクリームを、又他方のグループに処方例２のクリームと対照のクリームを割り振り、それぞれ左右の頬部に、本発明例又は対照のクリームを１日２回（朝、晩）、１ヵ月間塗布してもらった後、小じわ及び肌のはり、艶に対する改善効果を、以下の評価基準に基づいて評価した。
【００７２】
［評価基準］
Ａ：明らかに改善された
Ｂ：かなり改善された
Ｃ：僅かに改善された
Ｄ：変わらない
Ｅ：かえって悪くなった
【００７３】
［結果］
結果を表４に示す。なお、表４のＡ〜Ｅの各評価欄の数字は、被験者２０名中当該評価を行った被験者の割合（％）を示す。
【００７４】
【表４】

【００７５】
表４の結果から、本発明のヒッポファエ属植物抽出物からなるコラーゲン及びエラスチン産生促進剤を配合した化粧料が、小ジワや肌のハリ、艶を顕著に改善し、皮膚の老化防止に有効であることが明らかである。
【発明の効果】
本発明のグミ科ヒッポファエ属植物の抽出物は、皮膚に適用したとき、真皮マトッリクス成分のコラーゲン及びエラスチンの双方に対して、その産生を顕著に促進する作用を有し、しかも皮膚刺激性が少ないなど生体安全性にもすぐれ、皮膚の老化防止や肌荒れの改善等を目的としたコラーゲン及びエラスチン産生促進剤として用いて有用である。
又、このグミ科ヒッポファエ属植物抽出物からなるコラーゲン及びエラスチン産生促進剤を配合した本発明の化粧料は、皮膚の老化や肌荒れを防止し、皮膚を若々しく健全でハリのある状態に保持或いは改善する効果を有する。[0001]
BACKGROUND OF THE INVENTION
TECHNICAL FIELD The present invention relates to a collagen and elastin production promoter and a skin external preparation containing the same, and more particularly, when applied to the skin, the production of collagen and elastin, which are dermal matrix components, is promoted to make the skin youthful and firm. And an anti-aging cosmetic that contains such an agent and exhibits excellent effects for preventing skin aging and improving rough skin.
[0002]
[Prior art]
Skin aging is caused by internal factors such as inactivation of cell growth / differentiation associated with aging and a decrease in hormone secretion, as well as cell / tissue damage or inflammation based on active oxygen induced by exposure to sunlight (ultraviolet rays). This is a phenomenon that occurs when external factors such as occurrence are intricately intertwined.
Among these, aging of the skin based on internal factors reduces the amount of dermal matrix components such as collagen, elastin and hyaluronic acid produced by the decrease in the proliferation ability of dermal fibroblasts, and the skin tissue becomes flexible. It is caused by loss of sexuality and elasticity, or by deactivation of epidermal basal cells, resulting in deterioration of the stratum corneum barrier function, sebum secretion function, water retention function, etc. on the skin surface. Appears as wrinkles, sagging, rough skin, and rough skin.
[0003]
In order to prevent or ameliorate this skin aging and keep the skin in a youthful and firm state, it has been conventionally promoted the production of collagen and elastin as dermal matrix components by proliferating or activating fibroblasts. A variety of products such as placenta extract, soluble eggshell membranes, α-hydroxycarboxylic acids, hormones, and various vitamins have been proposed. However, these conventional components have biological irritation such as skin irritation and sensitization. However, there is a problem such as that there is a problem in safety against the above, or the production promoting ability of the matrix component is not sufficient, and a component that is not always satisfactory has not been found. Further, none of these conventional ingredients is known to show production promoting ability for both the dermal matrix constituent proteins of collagen and elastin.
[0004]
[Problems to be solved by the invention]
In view of the problems of the prior art as described above, the present inventors have the effect of strongly promoting the production of any of the two dermal matrix constituent proteins, collagen and elastin, and are excellent in biological safety. Ingredients, as well as these ingredients, when applied to the skin, are safe with little skin irritation, etc., and can prevent or maintain skin aging and rough skin to maintain or improve the skin in a youthful and firm state As a result of intensive studies and examinations for providing anti-aging cosmetics, it was found that an extract of a plant belonging to the genus Hippophae belonging to the family Gumiaceae meets these requirements, and the present invention was completed.
[Means for Solving the Problems]
[0005]
That is, the present invention first relates to a collagen and elastin production promoter containing as an active ingredient an extract of a plant belonging to the genus Hippophae.
Secondly, the present invention relates to an anti-aging cosmetic comprising a collagen and elastin production promoter containing an extract of a plant of the genus Hippophae as an active ingredient.
The anti-aging cosmetics referred to in this specification include quasi-drugs in addition to so-called cosmetics.
[0006]
Hereinafter, the present invention will be described in detail.
Examples of the plant belonging to the genus Hippophae used in the preparation of the collagen and elastin production promoters of the present invention include, for example, Rhamnoides (Hippophae rhhamnoides L.), Salifolia (Hippophae salisifolia L.), and Tibetana (Hippopae. neurocorpa L.) and the like.
Among them, the use of rhamnoides (Hhippophae ramnoides L.) is most preferable from the viewpoint of the ability of the obtained extract to promote collagen and elastin production.
[0007]
When preparing an extract of the genus Hippophae plant, there is no particular limitation on the extraction target part of the plant, and for example, an appropriate part such as a seed, a fruit, a leaf, a bark, a trunk, a root, or an above-ground part may be used. Of these, fruits are the most preferred. The extraction method can be performed by washing the extraction target sites with water, drying, chopping or pulverizing in advance, if necessary, and then contacting with an extraction solvent according to a conventional method such as a dipping method or a countercurrent extraction method.
[0008]
As an extraction solvent, water; lower alcohols such as methanol, ethanol, and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol, and octyldodecanol; many solvents such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin Monohydric alcohols; esters such as ethyl acetate, butyl acetate, methyl propionate and glyceryl trioctanoate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; carbonization such as n-hexane, toluene and chloroform Examples thereof include hydrogen-based solvents, which are used alone or in combination of two or more.
[0009]
Among these extraction solvents, from the viewpoint of the ability of the obtained extract to promote collagen and elastin production, and because it can be widely applied to cosmetics, in the present invention, water, lower alcohols or It is preferable to use a hydrophilic solvent such as polyhydric alcohols. In the case of using a hydrophilic solvent, preferred examples include water or lower alcohols, particularly ethanol alone, or a mixed solvent of water and lower alcohols, particularly ethanol, or water and polyhydric alcohols, particularly 1, Examples thereof include use of a mixed solvent with 3-butylene glycol or propylene glycol.
[0010]
When the mixed solvent is used, for example, if the mixed solvent is water and ethanol, the weight ratio (hereinafter the same) is 1: 1 to 10: 1, and the mixed solvent is water and 1,3-butylene glycol. If it is, it is preferably in the range of 1: 1 to 10: 1, or in the range of 1: 1 to 10: 1 if it is a mixed solvent of water and propylene glycol.
[0011]
In the preparation of the extract, the pH of the extract is not particularly limited, but is generally preferably in the range of 4 to 8. In this sense, if necessary, the above extraction solvent may be sodium hydroxide, sodium carbonate, water. You may mix | blend alkaline adjusters, such as potassium oxide, acidic adjusters, such as a citric acid, hydrochloric acid, phosphoric acid, and a sulfuric acid, and you may adjust so that it may become desired pH.
[0012]
Extraction conditions such as extraction temperature and time vary depending on the type and pH of the solvent used, the extraction site of the plant and the degree of shredding, for example, in the case of the immersion method using water as the extraction solvent, the extraction temperature is In general, it is in the range of 1 to 80 ° C, preferably 2 to 40 ° C, and the extraction time is 12 hours to 10 days in the case of cold extraction at 4 ° C, particularly 24 hours to 7 days, and 6 hours in the case of room temperature extraction. It is preferably ˜7 days, particularly 24 hours to 4 days, and 30 minutes to 24 hours, particularly 1 hour to 6 hours in the case of high-temperature extraction at 80 ° C.
[0013]
The extract solution thus obtained is generally adjusted to a pH of 4 to 8, and may be used as it is as a collagen and elastin production promoter, or may be used as an appropriate concentration by vacuum concentration or the like if necessary. Also good. Further, depending on the case, it can be pulverized according to a conventional method such as spray drying or freeze drying.
[0014]
The extract of the genus Hyphaphae plant of the present invention prepared as described above has an action of remarkably promoting the production of collagen and elastin of the dermal matrix component as shown in Test Examples later. Moreover, it has excellent biological safety such as low skin irritation, and is extremely useful as a collagen and elastin production promoter for the purpose of preventing skin aging and improving rough skin.
[0015]
Accordingly, the present invention also provides an anti-aging cosmetic comprising the collagen and elastin production promoter, and having an effect of maintaining or improving the skin in a youthful, healthy and firm state when applied to the skin. It is also what you do.
[0016]
In addition, it is known that fruit juice oil obtained from the fruit of the genus Hippophae genus, for example, Hippophae rhamnoides L., contains a considerable amount of α-tocopherol. Although it is known to show a certain effectiveness in preventing skin aging based on its antioxidant action, according to the results of our research, the extract of the present invention, for example, the extraction of Examples 1 to 6 described below It has been confirmed that the product is substantially free of α-tocopherol in any case. Therefore, it is clear that the collagen and elastin production promoting action and skin aging preventing action brought about by the extract of the genus Hippophae plant of the present invention are based on a novel component different from α-tocopherol. It is.
[0017]
Anti-aging cosmetics containing the collagen and elastin production promoter of the present invention include, for example, basic cosmetics such as emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, makeup press powders, cheeks, white powders Makeup cosmetics such as facial cleansers, body shampoos, soaps and other cleaning cosmetics, and bath agents, but of course are not limited thereto.
[0018]
The blending amount of the collagen and elastin production promoter (Gumphaceae hippophae plant extract) in the anti-aging cosmetic of the present invention is generally 0.002 to 1 in the case of a basic cosmetic as a solid content of the extract. 0.0% by weight, preferably in the range of 0.02 to 0.2% by weight, in the case of makeup cosmetics, generally 0.002 to 1.0% by weight, preferably 0.02 to 0.2% by weight. In the case of a range or a cleaning cosmetic, it is generally in the range of 0.002 to 10.0% by weight, preferably 0.02 to 7.0% by weight.
[0019]
In addition to the essential components collagen and elastin production promoter (Gumphaceae hippophae plant extract), the anti-aging cosmetics of the present invention include components usually used in cosmetics such as oily components, surfactants (synthetic systems) , Natural products), moisturizers, thickeners, antiseptic / bactericides, powder components, ultraviolet absorbers, antioxidants, pigments, fragrances, physiologically active components, and the like can be appropriately blended as necessary.
[0020]
Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.
[0021]
Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.
Also, as emulsifiers or emulsifiers, stevia derivatives such as enzyme-treated stevia, lecithin and derivatives thereof, lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, beans, millet, etc.), jujuiro (Rhamnaceae zyzyhus joazeiro) An extract or the like can also be blended.
[0022]
Examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, and sugars such as trehalose, lactic acid bacteria fermented rice, and mucopolysaccharides. (For example, hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, beech extract Products, seafood-derived collagen and derivatives thereof, various amino acids and derivatives thereof.
[0023]
Examples of thickeners include, for example, brown algae such as alginic acid, agar, carrageenan, fucoidan, green algae or red algae-derived components, extract of sand cucumber, pectin, locust bean gum, polysaccharides such as aloe polysaccharide, xanthan gum, tragacanth gum, guar gum, etc. Synthetic polymers such as gums, cellulose derivatives such as carboxymethylcellulose, hydroxyethylcellulose, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives, polyglutamic acid and its derivatives, etc. Is mentioned.
[0024]
Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzal chloride Luconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), 1,2-pentanediol, various essential oils, bark dry matter, and the like.
[0025]
Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powders, powders of cereals (rice, wheat, corn, millet, etc.), powders of beans (soybeans, red beans, etc.).
[0026]
Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .
[0027]
Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives, beech extract, rice extract and the like.
[0028]
Examples of physiologically active ingredients include whitening ingredients such as t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone derivatives, ellagic acid and derivatives thereof, resorcinol derivatives, sucha akuhi extract, yukinoshita extract, rice bran Extract, Rice bran extract hydrolyzate, Lactic acid bacteria fermented rice, Lactic acid bacteria fermented germinated rice, Lactic acid bacteria fermented cereals (wheat, beans, millet), White coconut hydrolyzed extract, Murasakixikib extract, Pandanus amari riforius Amaryllifolius Roxb.) extract, Arcangelicia flava Merrilli extract, chamomile extract (trade name: Chamomile ET), seaweed extract such as kombu, seaweed extract such as amamo, linoleic acid and So Derivatives or processed products (for example, liposomal linoleic acid), 2,5-dihydroxybenzoic acid derivatives, and the like.
[0029]
Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-O-α) -D-glucopyranosyl-L-ascorbine Acid) ascorbic acid sugar derivatives, acylated 6-positions of these ascorbic acid sugar derivatives (acyl groups are hexanoyl, octanoyl, decanoyl, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetra L-ascorbic acid tetrafatty acid esters such as lauric acid ester, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium and the like are hydroquinone derivatives such as arbutin (hydroquinone). -Β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like, and as resorcinol derivatives, for example, 4-n-butylresorcinol, 4-isoamylresorcinol and the like are 2,5-dihydroxybenzoic acid. Derivatives and For example, 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, etc., and nicotinic acid derivatives include, for example, nicotinic acid amide, nicotinic acid benzyl, etc. However, examples of the vitamin E derivative include vitamin E nicotinate and vitamin E linoleate, and examples of the α-hydroxy acid include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.
[0030]
In addition, as long as the effects of the collagen and elastin production promoter of the present invention are not impaired, other skin aging prevention / roughness improving agents may be used in combination as physiologically active ingredients. Collagen and derivatives thereof, elastin and derivatives thereof, nicotinic acid and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and derivatives thereof, vitamin E and derivatives thereof, allantoin, α- Herbal extracts such as hydroxy acids, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, pearl barley extract, chamomile extract, carrot extract, aloe extract, rice extract hydrolysate, rice bran extract Hydrolyzate, Fermented extract, Mitsuishikonbu extract, Ulva pertusa extract, seaweed extract, such as eelgrass, mulberry bark extract, Juazeiro (Rhamnaceae zizyphus joazeiro) have extracts.
[0031]
Next, the present invention will be described more specifically with reference to examples, formulation examples (cosmetic examples), and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all% are% by weight.
[0032]
Example 1. Preparation of collagen and elastin production promoter (1)
To 100 g of dry pulverized fruit of Hippophae rhamnoides fruit, 1000 g of purified water was added and extracted at 4 ° C. for 12 hours. The obtained extract was filtered to obtain 800 g of a pale yellow transparent extract solution (solid content: about 1.8%).
[0033]
Example 2 Preparation of collagen and elastin production promoter (2)
1000 g of purified water was added to 100 g of dry ground pulverized product of Hippophae rhamnoides, and the mixture was extracted at 80 ° C. for 1 hour. The obtained extract was filtered to obtain 700 g of a pale yellow transparent extract solution (solid content: about 3.0%).
[0034]
Example 3 FIG. Preparation of collagen and elastin production promoter (3)
1000 g of 30% 1,3-butylene glycol aqueous solution was added to 100 g of dried pulverized fruit of Hippophae rhamnoides, followed by extraction at room temperature (25 ° C.) for 24 hours. The obtained extract was filtered to obtain 650 g of a pale yellow transparent extract solution (solid content: about 2.7%).
[0035]
Example 4 Preparation of collagen and elastin production promoter (4)
1000 g of 50% aqueous ethanol solution was added to 100 g of dried pulverized fruit of Hippophae rhamnoides, followed by extraction at room temperature (25 ° C.) for 15 hours. The obtained extract was filtered to obtain 800 g of a pale yellow transparent extract solution (solid content: about 1.2%).
[0036]
Embodiment 5 FIG. Preparation of collagen and elastin production promoter (5)
300 ml of the extract solution obtained in the same manner as in Example 1 was concentrated to 30 ml and freeze-dried to obtain 5.5 g of extract powder.
[0037]
Example 6 Preparation of collagen and elastin production promoter (6)
In Example 1, a pale yellow transparent extract solution (solid content: about 2) was used in the same manner as in Example 1 except that the fruit of Hippophae salicifolia was used instead of the fruit of Hippophae rhamnoides. 0.0%) was obtained.
[0038]
Formulation Example 1 cream
[Component A] part
Liquid paraffin 5.0
Hexalan (Note 1) 4.0
Paraffin 5.0
Glyceryl monostearate 2.0
Polyoxyethylene (20) sorbitan monostearate 6.0
Butylparaben 0.1
(Note 1) Technoble Co., Ltd. glyceryl trioctanoate
[B component]
Extract solution of Example 1 5.0
Glycerin 5.0
Carboxymethyl monostearate 0.1
Moiston C (Note 2) 1.0
Amount of purified water totaling 100 parts
(Note 2) NMF component manufactured by Technoble Co., Ltd.
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain a cream.
[0039]
Formulation Example 2 cream
A cream was obtained in the same manner as in Formulation Example 1 except that the extract solution of Example 2 was used instead of the extract solution of Example 1 in the component B of Formulation Example 1.
[0040]
Formulation Example 3 Latex
[Component A] part
Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Extract solution of Example 1 5.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.
[0041]
Formulation Example 4 lotion
[Ingredient] part
Extract solution of Example 3 5.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume
Potassium hydroxide appropriate amount
Amount of purified water totaling 100 parts
The above ingredients were mixed to obtain a lotion.
[0042]
Formulation Example 5 Lotion
[Component A] part
Olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[B component]
Extract solution of Example 4 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Potassium hydroxide appropriate amount
Amount of purified water totaling 100 parts
[C component]
Perfume
After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes.
After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.
[0043]
Formulation Example 6 Latex
An emulsion was obtained in the same manner as in Formulation Example 3, except that the extract solution of Example 2 was used instead of the extract solution of Example 1 in Component B of Formulation Example 3.
[0044]
Formulation Example 7 Latex
An emulsion was obtained in the same manner as in Formulation Example 3 except that the extract solution of Example 3 was used instead of the extract solution of Example 1 in the B component of Formulation Example 3.
[0045]
Formulation Example 8 Latex
An emulsion was obtained in the same manner as in Formulation Example 3 except that the extract solution of Example 6 was used instead of the extract solution of Example 1 in the B component of Formulation Example 3.
[0046]
Formulation Example 9 Latex
[Component A] part
Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Extract solution of Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.
[0047]
Example 10 Latex
Other than using 2.0 parts of L-ascorbic acid-2-phosphate magnesium instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the B component of Formulation Example 9. An emulsion was obtained in the same manner as in Formulation Example 9.
[0048]
Formulation Example 11 Latex
Other than using 2.0 parts of L-ascorbic acid-2-phosphate sodium in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the B component of formulation example 9. An emulsion was obtained in the same manner as in Formulation Example 9.
[0049]
Formulation Example 12. Latex
In the component B of formulation example 9, an emulsion was obtained in the same manner as formulation example 9 except that 2.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide. It was.
[0050]
Formulation Example 13 Latex
In the B component of Formulation Example 9, instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide, a rice bran extract hydrolyzate (trade name “Gracenow * manufactured by Technoble Co., Ltd.) Emulsion was obtained in the same manner as in Formulation Example 9, except that 5.0 parts of “Snow * HP”, solid concentration 3.5%) was used.
[0051]
Formulation Example 14. Latex
In the B component of Formulation Example 9, instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide, white coconut extract (trade name “Sinablanca-WH” manufactured by Technoble Co., Ltd., An emulsion was obtained in the same manner as in Formulation Example 9 except that 5.0 parts of a solid content concentration of 1.0% were used.
[0052]
Formulation Example 15. Latex
Formulation example 9 except that 1.0 part of γ-amino-β-hydroxybutyric acid is used in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the component B of formulation example 9. In the same manner, an emulsion was obtained.
[0053]
Formulation Example 16. Press powder
[Component A] part
Bengala 0.5
Yellow iron oxide 1.5
Black iron oxide 0.1
Titanium oxide 10.0
Nylon powder 4.0
Amount of sericite that makes up 100 parts
Mica 23.0
Talc 25.0
Extract powder of Example 5 0.2
[B component]
Squalane 1.0
Methylpolysiloxane 4.0
Propylparaben 0.1
Dehydroacetic acid 0.1
Liquid paraffin 2.0
Perfume
The above A component and B component were mixed and agitated, mixed, then passed through a 200 mesh Tyler mesh sieve, and the resulting mixed powder was cast into a mold to obtain a pressed powder.
[0054]
Formulation Example 17. Liquid foundation
[Component A] part
Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B component]
Extract solution of Example 2 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Amount of purified water totaling 100 parts
[C component]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount
The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.
[0055]
Formulation Example 18. Cream foundation
[Component A] part
Stearic acid 5.0
Cetanol 2.0
Glyceryl monostearate 3.0
Liquid paraffin 5.0
Squalane 3.0
Isopropyl myristate 8.0
Polyoxyethylene (20) glyceryl monostearate 2.0
Propylparaben 0.1
[B component]
Extract solution of Example 3 5.0
Sorbitol 3.0
1,3-butylene glycol 5.0
Triethanolamine 1.5
Methylparaben 0.1
Amount of purified water totaling 100 parts
[C component]
Titanium oxide 8.0
Talc 2.0
Kaolin 5.0
Bentonite 1.0
Coloring pigment appropriate amount
[D component]
Fragrance 0.3
Component C was mixed and pulverized with a pulverizer. The component B was mixed, the pulverized component C was added thereto, and the mixture was uniformly dispersed with a colloid mill. The components A and B and C that were uniformly dispersed were each heated to 80 ° C., and then the components A were added to the components B and C with stirring, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, D component was added and stirred and mixed, and also it cooled to 30 degrees C or less, stirring, and obtained the cream foundation.
[0056]
Formulation Example 19. Body shampoo
[Component A] part
N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B component]
Extract solution of Example 3 5.0
1,3-butylene glycol 2.0
Amount of purified water totaling 100 parts
After each component A and component B was heated to 80 ° C. and uniformly dissolved, component B was added to component A, and stirring was continued to cool to room temperature to obtain a body shampoo.
[0057]
Formulation Example 20. Soap
[Component A] part
Hardened castor oil 26.0
Coconut oil 10.0
Olive oil 4.0
[B component]
Sodium hydroxide 6.0
Sugar 10.0
Glycerin 5.0
Extract powder of Example 5 5.0
Amount of purified water totaling 100 parts
[C component]
Ethanol 20.0
Perfume
The components A and B were each heated to 80 ° C. and dissolved uniformly, and then the component B was added to the component A to saponify. This was cooled to 50 ° C. with stirring, and component C was added. This was poured into a mold and cooled, and then dried at room temperature for several days. A sufficiently dried product was taken out from the mold to obtain soap.
[0058]
Test Example 1 Collagen production promoting action
Using the extract solution obtained in Example 1 as a sample, the ability of the hippophae plant extract of the present invention to promote collagen production was examined.
[0059]
[Test method]
Normal human skin-derived fibroblasts (NB1RGB) were added to 1.0 × 10 6 in a 96-well microplate containing 0.5% NCS-containing Eagle MEM medium. ⁴ After seeding so that it becomes a piece / hole and culturing at 37 ° C. for 24 hours, the sample (the extract solution of Example 1) is placed in the medium so that the concentration becomes 2.5 or 5.0% (as a solution). After addition, the cells were further cultured for 5 days. Next, the culture solution was removed, 0.04% Fast Green, 0.05% Sirius red solution was added, and intracellular collagen and non-collagen were stained and washed thoroughly. Next, extraction was performed with 0.1% NaOH-containing methanol, and the amount of collagen was measured at 540 nm and the amount of non-collagen was measured at 630 nm. L-ascorbic acid-2-phosphate magnesium (APM) was used as a positive control.
[0060]
[result]
The results are shown in Table 1.
[Table 1]

[0061]
As shown in Table 1, the Hippophae plant extract of the present invention has a high collagen production promoting action comparable to APM.
[0062]
Test Example 2 Elastin production promoting effect
The extract solution obtained in Example 1 was used as a sample, and the elastin production promoting ability of the Hippophae plant extract of the present invention was examined.
[0063]
[Test method]
Normal human skin-derived fibroblasts (NB1RGB) were added to 1.0 × 10 6 in a 96-well microplate containing 0.5% NCS-containing Eagle MEM medium. ⁴ After seeding so as to form individual pieces / hole and culturing at 37 ° C. for 24 hours, the sample (the extract solution of Example 1) is adjusted to a concentration of 2.5 or 5.0% (as a solution) in the medium After addition, the cells were further cultured for 7 days. The amount of elastin secreted into the culture supernatant was measured by ELISA using an anti-human elastin antibody.
[0064]
[result]
The results are shown in Table 2.
[Table 2]

[0065]
As shown in Table 2, the hippophae plant extract of the present invention significantly promoted elastin production as compared with the case where no sample was added.
[0066]
Test Example 3 Skin irritation
[sample]
(1) The extract solution obtained in Example 1 was kneaded with JP Hydrophilic Petrolatum so as to have a concentration of 5% (sample of the present invention).
(2) JP Hydrophilic Petrolatum (control)
[0067]
[Test method]
5 adult males aged 20 to 50 years old as test subjects, the inner side of each upper arm was wiped with ethanol to remove sebum, and 0.2 g of each sample was applied to the aluminum plate of the fin chamber. Was affixed. After 24 hours, the fin chamber was removed, and the degree of skin irritation was determined by the method and criteria described below.
[Judgment]
After 1 hour, 24 hours and 48 hours after patch removal, the condition of erythema and edema at the applied site was visually determined based on the following “skin irritation criteria by the dreze method”, and the average value of 5 subjects was determined. Asked.

[0068]
[result]
The results are shown in Table 3.
[Table 3]

[0069]
As is apparent from the results in Table 3, the extract of the Hippophae plant has almost no irritation to the skin and is extremely excellent in biological safety.
[0070]
Test Example 4 Panel test
About the anti-aging cosmetic containing the collagen and the elastin production promoter of this invention, the effect with respect to skin was investigated by the panel test.
[sample]
(1) Cream of Formulation Example 1 (Invention Example)
(2) Cream of Formulation Example 2 (Example of the present invention)
(3) Cream obtained by using purified water instead of the extract solution of Example 1 in the formulation example 1 (control)
[0071]
[Test method]
Randomly extracted 40 women aged 18-50 were divided into two groups of 20 subjects, one group containing the cream of the prescription example 1 and the control cream, and the other group of the prescription example 2 After allocating the cream and the control cream and applying the example of the present invention or the control cream to the left and right cheeks twice a day (morning and evening) for 1 month, fine lines and skin spots, gloss The improvement effect on was evaluated based on the following evaluation criteria.
[0072]
[Evaluation criteria]
A: Clearly improved
B: Remarkably improved
C: Slightly improved
D: Unchanged
E: It got worse
[0073]
[result]
The results are shown in Table 4. In addition, the number of each evaluation column of AE of Table 4 shows the ratio (%) of the test subject who performed the said evaluation among 20 test subjects.
[0074]
[Table 4]

[0075]
From the results shown in Table 4, the cosmetic comprising the collagen of the Hippophae genus extract of the present invention and the elastin production promoter markedly improves fine wrinkles, skin firmness and gloss, and is effective in preventing skin aging. It is clear that there is.
【The invention's effect】
The Gumiaceae Hippophae plant extract of the present invention, when applied to the skin, has the effect of remarkably accelerating the production of both dermal matrix components collagen and elastin, and has little skin irritation. It is excellent in biological safety, and is useful as a collagen and elastin production promoter for the purpose of preventing skin aging and improving rough skin.
In addition, the cosmetic of the present invention containing the collagen and elastin production promoter composed of this gummy family Hippophae plant extract prevents skin aging and rough skin, and keeps the skin in a youthful, healthy and firm state. Or it has the effect of improving.

Claims (4)

Collagen and elastin production promoter containing an extract of a plant of the genus Hippophae as an active ingredient. The collagen and elastin production promoter of Claim 1 which uses the extract of Hippophae rhamnoides L. as an active ingredient. The collagen and elastin production promoter according to claim 1 or 2, comprising an extract of a fruit of a Hippophae plant as an active ingredient. An anti-aging cosmetic comprising the collagen and elastin production promoter according to claim 1.