JPH09208484A - Active oxygen-eliminator and composition containing the same - Google Patents

Active oxygen-eliminator and composition containing the same

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Publication number
JPH09208484A
JPH09208484A JP3730796A JP3730796A JPH09208484A JP H09208484 A JPH09208484 A JP H09208484A JP 3730796 A JP3730796 A JP 3730796A JP 3730796 A JP3730796 A JP 3730796A JP H09208484 A JPH09208484 A JP H09208484A
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oxygen
active
plant
eliminating
agent
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JP3730796A
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Japanese (ja)
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Toshiyuki Fukuda
Hirotaka Miyazaki
博隆 宮崎
寿之 福田
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Pola Chem Ind Inc
ポーラ化成工業株式会社
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Abstract

PROBLEM TO BE SOLVED: To obtain an active oxygen-eliminator containing the essence of a plant belonging to the genus Hippophae in the family Elaeagnaceae, capable of sufficiently eliminating active oxygen generated in living bodies, and high in safety.
SOLUTION: This active oxygen-eliminating agent contains the essence of a a plant (e.g, Hippophae rhamnoides) belonging to the genus Hippophae (generally called as 'saci') in the family Elaeagnaceae. The plant essence includes a processed plant product obtained e.g. by drying, grinding or kneading the plant itself into a paste, an extraction solution obtained by extracting the plant or the processed plant with a solvent, an extract obtained by removing the solvent from the extraction solution, and its purified product. The active oxygen-eliminating agent may be prepared into compositions such as medicines, foods, beverages or cosmetics. The active oxygen- eliminating agent is suitable for treating and preventing ageing, allergies, cardiac infarction, hepatopathy, dementia, rheumatism, etc. The content of the active oxygen- eliminating agent in the cosmetic or food is preferably 0.1-1wt.%, and the active oxygen-eliminating agent as the medicine is orally administered at a daily dose of 10-100mg for an adult in one to several portions or injected at a daily dose of 5-500mg for an adult.
COPYRIGHT: (C)1997,JPO

Description

【発明の詳細な説明】 DETAILED DESCRIPTION OF THE INVENTION

【0001】 [0001]

【発明の属する技術分野】本発明は活性酸素消去剤及びそれを含有する皮膚外用剤や食品や医薬品などの組成物に関する。 The present invention relates to relates to compositions such as skin external preparations and food and medicines containing active oxygen scavenger and it.

【0002】 [0002]

【従来の技術】一般的に、活性酸素が生体に及ぼす影響としては、コラーゲン線維の架橋や、DNA螺旋の部分開裂、連鎖的ラジカルの発生による組織の損傷が挙げられ、その結果としてシワや弾力消失、脱毛といった皮膚や生体の老化、気管支喘息等のアレルギー反応の惹起とヒスタミン放出による炎症の惹起、虚血性疾患である心筋梗塞に於ける平滑筋の損傷、肝臓障害などの疾患の悪化、又、脳組織の破壊による痴呆の誘発等が引き起こされることが知られている。 2. Description of the Related Art In general, as the effect of active oxygen on biological, crosslinking or collagen fibers, part cleavage of the DNA helix, include tissue damage due to the occurrence of a chain radicals, wrinkles and elasticity as a result loss, skin aging and biological like hair loss, induction of inflammation by inducing the release of histamine allergic reactions such as bronchial asthma, damage myocardial infarction in smooth muscle ischemic diseases, aggravation of diseases such as liver damage, and it is known that induction or the like of dementia by disruption of brain tissue is caused. 更に、詳細な原因は不明であるがリューマチの発症にも活性酸素が関与していると言われている。 Furthermore, detailed reason is is unclear is said that active oxygen to the onset of rheumatism is involved.

【0003】従って、生体内に於いて活性酸素の発生を抑制することは、これらの疾患を治療或いは予防する点で非常に重要なことであり、このため、従来より生体内に発生した活性酸素を消去する作用のある物質の探索が広く行われてきていた。 [0003] Therefore, to suppress the generation of active oxygen at the in vivo is that these diseases to the treatment or in terms of prevention very important, Therefore, active oxygen generated in conventionally vivo search of a substance of action of erasing had been widely performed.

【0004】例えば、この様な作用を有する薬剤として、従来より用いられてきたものとしては、天然物由来のものでは、脂溶性のトコフェロール(ビタミンE)、 [0004] For example, as drugs having such action, those which have been conventionally used, those derived from natural products, fat-soluble tocopherol (vitamin E),
水溶性のアスコルビン酸(ビタミンC)が挙げられ、合成化合物では、BHT(ブチルヒドロキシトルエン)、 Water-soluble ascorbic acid (vitamin C) and the like, in the synthetic compound, BHT (butylhydroxytoluene),
BHA(ブチルヒドロキシアニリン)等が挙げられる。 BHA (butyl hydroxy aniline), and the like.
しかし、これらの薬剤は活性酸素消去作用が充分ではなく、合成化合物に於いては、BHTもBHAも発癌性の疑いが持たれており、何れも活性酸素消去剤としては実用的とは言い難かった。 However, these drugs are not sufficient active oxygen scavenging activity, is at the synthetic compounds, BHT also BHA has also been given suspected carcinogenic, both as the active oxygen scavenging agents say that practically hardly It was.

【0005】又、最近では、充分な薬効と安全性を求めて、生薬抽出物から活性酸素消去作用を有する物質を得ようとする試みも数多く為されており、例えば、特開昭 60−224629号、特開昭 61−24522号、 [0005] Also, recently, seeking sufficient efficacy and safety, have been made also numerous attempts to obtain a substance having an active oxygen scavenging action from herbal extracts, for example, JP 60-224629 JP, JP-A-61-24522,
特開平2−193930号、特開平2−243632 JP-A-2-193930, JP-A-2-243632
号、特開平2−264727号、特開平3−15362 JP, JP-A-2-264727, JP-A-3-15362
9号、特開平3−221587号、特開平4−6934 No. 9, JP-A-3-221587, JP-A 4-6934
3号、特開平4−202138号、特開平4−2470 No. 3, JP-A-4-202138, JP-A 4-2470
10号、等これらは何れも生薬由来の活性酸素消去作用を有する物質を利用したものである。 No. 10, etc. These are the both using a substance having active oxygen scavenging action from crude drugs. しかし、これらの生薬抽出物では、安全性に問題がないものの、活性酸素消去作用の点から言えば、未だ充分なものは得られていなかった。 However, these crude drug extracts, although there is no problem in safety, speaking in terms of the active oxygen scavenging action, have not been obtained yet sufficient ones.

【0006】更に、生体内の酵素の一つスパーオキサイドデスムターゼ(SOD)を投与することにより、生体内に発生する活性酸素を消去する試みも為されてきているが、SODはタンパク質であるため、その入手が困難であるばかりでなく、消化されてしまうが故に、経口投与は不可能であり、又、注射による投与に於いても、血中半減期が短く満足の行くものではなかった。 Furthermore, by administering one of the enzymes in the body superoxide dismutase (SOD), but have been also made an attempt to erase the active oxygen generated in the living body, since SOD is a protein not only the availability is difficult, because it would be digested, oral administration is not possible, also at the administration by injection, was not life blood half goes the short and satisfactory.

【0007】一方、グミ科ヒッポファエ属(Hippopha [0007] On the other hand, elaeagnaceae Hippofae genus (Hippopha
e)の植物の植物体に、癌を治療或いは予防する作用を有する成分が含まれていることは知られていたが、活性酸素を消去する作用を有することは全く知られていなかった。 The plant of the plant of e), it has been known that contains a component having an effect of treating or preventing cancer, was not known at all to have the effect of erasing the active oxygen. 更に、化粧料、医薬品或いは食品等に含有させて、上述した様な様々な疾患の予防や治療、老化の防止、改善に用いる試みはされていなかった。 Furthermore, the cosmetic, be contained in pharmaceutical products or foods, prevention and treatment of various diseases such as mentioned above, prevention of aging, has not been the attempt to use the improvement.

【0008】 [0008]

【発明が解決しようとする課題】本発明は、かかる状況を鑑みて為されたものであり、生体内に発生する活性酸素を充分に消去する作用を有し、且つ、安全性が高い活性酸素消去剤及びこれを含有する組成物を提供することを課題とする。 [SUMMARY OF THE INVENTION The present invention has been made in view of such circumstances, have the effect of sufficiently erased the active oxygen generated in the living body, and is highly safe active oxygen and to provide a scavenger and compositions containing the same.

【0009】 [0009]

【課題を解決するための手段】本発明者らは上記課題を解決するために、活性酸素消去作用を指標に各種植物由来の化合物を広くスクリーニングした結果、グミ科ヒッポファエ属の植物のエッセンスが優れた活性酸素消去作用を有することを見いだし、本発明を完成させるに至った。 The present inventors have SUMMARY OF THE INVENTION To solve the above problem, a result of the wide screen compounds for various plant-derived active oxygen scavenging action as an index, excellent essence of elaeagnaceae Hippofae plant of the genus It found to have active oxygen scavenging action and, thereby completing the present invention. ここで、本発明で言うエッセンスとは、植物体そのものを乾燥、粉砕、ペースト化等加工した、植物体の加工物、植物体或いは植物体の加工物を溶媒で抽出した、 Here, the essence in the present invention, drying the plants themselves, milled, and processed paste, etc., processed product of the plant bodies were extracted workpieces of a plant or plant with a solvent,
或いはそれらから溶媒を除去した抽出、抽出物をカラムクロマトグラフィー、液液抽出、限外濾過等で精製した精製物を含んだものを意味する。 Or extraction and the solvent was removed therefrom, the extract column chromatography, liquid-liquid extraction is one which contains the purified product was purified by ultrafiltration or the like.

【0010】(1)本発明の活性酸素消去剤 本発明の活性酸素消去剤はサーチと総称されるグミ科ヒッポファエ属の植物の植物体のエッセンスからなる。 [0010] (1) active oxygen scavenger of active oxygen eliminator invention of the present invention consists of essences of plants elaeagnaceae Hippofae genus of plants known collectively as the search. グミ科ヒッポファエ属の植物としては、サリシフォリア(H.salicifolia)、チベターナ(H.thibetana)、ネウロコルパ(H.neurocorpa)、ラムノイデス(H.rhamnoid The elaeagnaceae Hippofae plants of the genus, Sarishiforia (H.salicifolia), Chibetana (H.thibetana), Neurokorupa (H.neurocorpa), Ramunoidesu (H.rhamnoid
es)等が挙げられ、本発明はこれらの何れもが使用可能である。 es), and the like, the present invention any of these can be used. この中で最も一般的なものはラムノイデス(H. The most common is Ramunoidesu (H. In this
rhamnoides)である。 It is a rhamnoides). これらは単独で用いても良いし、 These may be used alone,
2種以上を組み合わせて用いても良い。 It may be used in combination of two or more. これらの植物体より、抽出物を得るには、植物体又はその加工物に1〜 From these plants, to obtain an extract, 1 in plant or processed product thereof
10倍量の溶剤を加え、室温であれば数日、沸点付近の温度であれば数時間浸漬すればよい。 10 volumes of the solvent added, a few days if room temperature, may be soaked for several hours as long as the temperature around the boiling point. 溶媒としては、極性溶媒が好ましく、メタノールやエタノール等のアルコール類、酢酸エチルや蟻酸メチル等のエステル類、アセトニトリル等のニトリル類、ジエチルエーテルやテトラヒドロフラン等のエーテル類、クロロホルムや塩化メチレン等のハロゲン化炭化水素類、アセトンやメチルエチルケトン等のケトン類等が例示でき、これらから選ばれる1種又は2種以上を用いればよい。 As the solvent, preferably a polar solvent, methanol or alcohols such as ethanol, esters such as ethyl or methyl formate acetate, nitriles such as acetonitrile, ethers such as diethyl ether or tetrahydrofuran, chloroform and halogenated such as methylene chloride hydrocarbons, can be exemplified ketones such as acetone and methyl ethyl ketone and the like, may be used one or more selected from these. 本発明の活性酸素消去剤は、植物体、植物体の加工物、植物体の抽出物、 Active oxygen eliminator of the present invention, plant, processed product of a plant, an extract of a plant,
該抽出物の精製物からなる。 Consisting of a purified product of the extract. 植物体の部位としては、特段の限定はされないが、果実が最も好ましい。 The site of the plant, but are not otherwise limited, the fruit is most preferred. これらの内最も好ましいものは果実の水抽出物である。 Most preferred of these is water extract of the fruit. 果実より作られる本発明の活性酸素消去剤は、果実が食用であることから、高い安全性が期待できる。 Active oxygen eliminator of the present invention made from the fruit, since the fruit is edible, high safety can be expected.

【0011】(2)本発明の活性酸素消去剤を含有する組成物 本発明の組成物は、上記活性酸素消去剤を常法により配合したものであり、具体的には、化粧料、医薬品、食品、飲料等が例示できる。 [0011] (2) The composition of the compositions according to the invention containing the active oxygen scavenger of the present invention is obtained by blending by a conventional method the active oxygen eliminator, specifically, cosmetics, pharmaceuticals, food, beverages and the like.

【0012】本発明の活性酸素消去剤を化粧料に剤形化する場合、化粧料剤形としては特に限定されないが、化粧水、乳液、クリーム等の基礎化粧料、ファンデーション、アンダーメークアップ、白粉等のメークアップ料、 [0012] When formulated active oxygen scavenging agent cosmetic agent of the present invention is not particularly restricted but includes cosmetic formulations, skin lotion, milky lotion, foundation cosmetics such as creams, foundations, under makeup, white powder make-up fee and the like,
ヘアトニック、ヘアリキッド、シャンプー、リンス等の頭髪用化粧料等が例示できる。 Hair tonic, hair liquid, shampoo, cosmetics and the like for hair rinse, etc. can be exemplified. これらの製剤は、本発明の活性酸素消去剤と化粧料用の任意成分を常法により製剤化する事により得られる。 These preparations are obtained by formulating in a conventional manner any component for active oxygen scavenging agents and cosmetic of the present invention. 任意成分としては、ワセリン、流動パラフィン等の炭化水素類、ホホバ油、カルナバワックス等のエステル類、オリーブ油、牛脂等のトリグリセライド類、ステアリルアルコール、ベヘニルアルコール等のアルコール類、ステアリン酸、ベヘン酸等の脂肪酸類、グリセリルモノステアレート、ポリオキシエチレンステアリン酸、ポリオキシエチレン硬化ヒマシ油、ポリオキシエチレンステアリルエーテル等のノニオン界面活性剤、石鹸、硫酸エステル等のアニオン界面活性剤、ステアリルアミン等のカチオン界面活性剤、アルキルベタイン等の両性界面活性剤、グリセリン、プロピレングリコール等の多価アルコール類、増粘剤、防腐剤、紫外線吸収剤、酸化防止剤等が挙げられる。 The optional components, petrolatum, hydrocarbons such as liquid paraffin, jojoba oil, esters such as carnauba wax, olive oil, triglycerides of beef tallow, stearyl alcohol, and behenyl alcohol, stearic acid, fatty acids such as behenic acid s, glyceryl monostearate, polyoxyethylene stearate, polyoxyethylene hydrogenated castor oil, polyoxyethylene stearyl ether nonionic surfactant such as, soaps, anionic surfactants such as sulfuric ester, cationic surfactants of stearylamine agents, amphoteric surface active agents such as alkyl betaines, glycerin, polyhydric alcohols such as propylene glycol, thickening agents, preservatives, ultraviolet absorbers, antioxidants, and the like. 更に、 In addition,
SOD等の活性酸素消去作用を有する物質を、本発明の活性酸素消去剤と共に配合しても構わない。 A substance having an active oxygen scavenging action of SOD like, may be formulated with an active oxygen eliminator of the present invention.

【0013】化粧料に於いては、本発明の活性酸素消去剤は、活性酸素を消去することによって、紫外線照射で引き起こされるコラーゲン架橋に起因するシワを防いだり、脱毛を予防したり、体臭等の好ましくない匂いの発生を防いだりする作用を有する。 [0013] In cosmetics, the active oxygen eliminator of the present invention, by erasing the active oxygen, Dari prevent wrinkles due to collagen crosslinking caused by UV irradiation, or prevent hair loss, body odor, etc. has the effect of Dari prevent the occurrence of undesirable odors. 化粧料に於ける、本発明の活性酸素消去剤の好適な含有量は、この作用が効果的に発現する、0.01〜10重量%である。 In the cosmetic, suitable content of active oxygen eliminator of the present invention, this effect is effectively expressed, from 0.01 to 10% by weight. 即ち、 In other words,
0.01%未満ではこれらの効果は発現しない場合があり、10重量%を越えて配合しても効果が頭打ちになり経済的でない場合が多い。 Is less than 0.01% may these effects do not express, often effect though the amount is more than 10 wt% is not economical become plateaued. 更に好ましい含有量は0.1 Further preferable content is 0.1
〜1重量%である。 A 1% by weight.

【0014】本発明の活性酸素消去剤を医薬品として製剤化する場合、剤形は特に限定されないが、例えば、注射剤、散剤、顆粒剤、錠剤、カプセル剤、液剤等通常用いられている各種剤形へ、賦形剤、結合剤、崩壊剤、滑沢剤、矯味矯臭剤、増量剤、被服剤等の医薬品で通常用いられる任意成分と共に、通常の方法に従って剤形化できる。 [0014] When formulated as a pharmaceutical active oxygen scavenger of the present invention, dosage is form is not particularly limited, for example, injections, powders, granules, tablets, capsules, various agents commonly used liquid or the like to form, excipients, binders, disintegrants, lubricants, flavoring agents, bulking agents, together with optional components normally used in pharmaceuticals, such as clothing agents, dosage forms in accordance with routine methods.

【0015】上記医薬品の投与量に関しては、疾患の種類、症状、患者の年令、体重等により異なるが、成人1 [0015] With respect to the dosage of the medicament, the type of disease, condition, age of the patient, varies depending body weight and the like, an adult 1
人1日あたり、本化合物の量として、10mg〜100 Person per day, as the amount of the compound, 10mg~100
0mgを1回ないしは数回に分けて経口投与するか、5 Or orally administered divided 0mg once or several times, 5
mg〜500mgを注射で投与するのが適当である。 It is appropriate to administer at the injection Mg~500mg. 注射剤の投与方法としては、静脈内投与、動脈内投与、門脈内投与、腹腔内投与、筋肉内投与、皮下投与等が例示できる。 The method of administering the injections, intravenous, intraarterial, intraportal administration, intraperitoneal administration, intramuscular administration, subcutaneous administration and the like.

【0016】本発明の活性酸素消去剤を食品に配合する場合、特に留意することはなく、種々の食品へ、食品で用いられる任意成分と共に配合できる。 [0016] The active oxygen scavenger of the present invention when formulated into food, not be particularly noted that the various foods can be formulated with any component used in the food. 例えば、キャンディー、やグミ、ゼリーと言ったお菓子類やジュースの様なドリンク類、パン等の主食が例示できる。 For example, candy, and gummy, such drinks of sweets and juice said that the jelly, the staple food, such as bread can be exemplified. 配合量は食品の種類により異なるが、食品の味を損なわず、活性酸素消去作用が期待できる0.01〜10重量%が好ましく、更に好ましくは0.1〜1重量%である。 The amount varies depending on the type of food, without impairing the taste of the food, preferably from 0.01 to 10 wt% of active oxygen scavenging action can be expected, more preferably from 0.1 to 1 wt%.

【0017】(3)作用 本発明の活性酸素消去剤及びこれを含有する組成物は、 [0017] (3) acting active oxygen eliminator and compositions containing same of the present invention,
その有効成分である本化合物及び/又はその塩の優れた活性酸素消去作用により、上述したような活性酸素が関与しているとされている、紫外線照射に誘発されるコラーゲン架橋に起因するシワの形成、体臭の発生、脱毛、 The excellent active oxygen scavenging action of the compound and / or salt thereof is an effective ingredient, the active oxygen as described above are to be involved, the wrinkles caused by collagen crosslinking induced to ultraviolet radiation formation, the generation of body odor, hair loss,
炎症、老人性痴呆、心筋梗塞等の虚血性疾患、或いはアレルギー性疾患、肝臓障害、リューマチ等様々な疾病の治療や皮膚などの生体老化の改善に対して有効に働くものである。 Inflammation, in which work effectively against senile dementia, ischemic diseases such as myocardial infarction, or allergic diseases, liver disorders, improvement of biological aging, such as therapeutic and skin of rheumatism such as various diseases.

【0018】又、本発明の活性酸素消去剤及びこれを含有する組成物は、上記疾病や生体老化の老化の予防のためにも有効に使用できる。 [0018] Further, active oxygen scavenger and compositions containing same of the present invention can be effectively used for the prevention of aging of the diseases and biological aging. これは、活性酸素消去作用を有する本化合物及び/又はその塩を、予め生体内に存在させることにより、生体内で発生した活性酸素を素早く消去し、無毒化することが出来るためである。 This is the compound having an active oxygen scavenging action and / or a salt thereof, by the presence in advance in the living body, the active oxygen generated in vivo erased quickly, because that can be detoxified.

【0019】 [0019]

【発明の実施の態様】以下に例を挙げて発明の実施の形態について詳細に説明するが、本発明がこれら例にのみ限定を受けないことは言うまでもない。 For be described in detail embodiments of the invention by way of example: [aspect of the practice of the present invention, it goes without saying that the present invention does not undergo limited thereto example.

【0020】例1:製造例 サーチ果実の果肉1Kgをフードプロセッサーで粉砕した後、フィルトプレスでエキスを絞りこれを凍結乾燥した。 [0020] Example 1: After grinding the flesh 1Kg of Production Example search fruit in a food processor, and it squeezed the extract in Filtration press and freeze-dried. これに水1lを加え、濾過した後濾液を凍結乾燥し51gの活性酸素消去剤1を褐色粉末として得た。 This water 1l was added to give the active oxygen eliminator 1 of the filtrate after filtration and freeze-dried 51g as a brown powder.

【0021】例2:製造例 サーチ果実の果肉1Kgをフードプロセッサーで粉砕した後、フィルトプレスでエキスを絞りこれを凍結乾燥した。 [0021] Example 2: After grinding the flesh 1Kg of Production Example search fruit in a food processor, and it squeezed the extract in Filtration press and freeze-dried. これに50%エタノール水溶液1lを加え、濾過した後濾液を凍結乾燥し47gの活性酸素消去剤2を褐色粉末として得た。 Thereto 50% aqueous ethanol solution 1l was added, the filtrate was filtered and lyophilized to give the active oxygen eliminator 2 of 47g as a brown powder.

【0022】例3:製造例 サーチ果実の果肉1Kgをフードプロセッサーで粉砕した後、フィルトプレスでエキスを絞りこれを48℃で真空乾燥した。 [0022] Example 3: After grinding the flesh 1Kg of Production Example search fruit in a food processor, and dried under vacuum at this 48 ℃ squeezed the extract in Filtration press. これに水1lを加え、濾過した後濾液を凍結乾燥し52gの活性酸素消去剤3を褐色粉末として得た。 This water 1l was added to give the active oxygen scavenger 3 of the filtrate after filtration and freeze-dried 52g as a brown powder.

【0023】例4(キャンディー) 表1の処方に従って、キャンディーを作成した。 [0023] In accordance with the formulation of Example 4 (candy) Table 1, it was to create a candy. 即ち、 In other words,
A成分を150℃で加熱溶解し、120℃に冷却後、B The A component was heated and dissolved at 0.99 ° C., cooled to 120 ° C., B
成分を添加、攪はん後、均一にしたものを成形後冷却しキャンディーを得た。 Added components, after 攪 solder to give the candy to cool after molding a material obtained by uniformly.

【0024】 [0024]

【表1】 [Table 1]

【0025】例5(グミ) 表2の成分を110℃で加熱溶解し、別途膨潤させたB [0025] Example 5 (gummy) Table 2 components were heated and dissolved at 110 ° C., and allowed to separately swelling B
成分を添加し、更にC成分を添加し、型に流し込み、一昼夜放置後型から外してグミを得た。 Added components, further adding component C, poured into a mold to obtain a gummy removed from overnight after standing type.

【0026】 [0026]

【表2】 [Table 2]

【0027】例6(ジュース) 表3の成分を良く攪拌可溶化し、滅菌、無菌充填、密閉してジュースを製造した。 [0027] Example 6 (juice) a component of Table 3 may stirring solubilization, sterilization, aseptic filling, to produce a juice was sealed.

【0028】 [0028]

【表3】 [Table 3]

【0029】例4(ホットケーキ) 表4の成分を良く混ぜ合わせ、油を引いたフライパンで焼き上げホットケーキを作成した。 [0029] Example 4 was mixed well the components of the (hot cake) Table 4, it was to create a hot cake baked in a frying pan minus the oil.

【0030】 [0030]

【表4】 [Table 4]

【0031】例7(顆粒剤) 表5のA成分を良く混合し、これに100mlの20% [0031] Example 7 A composition of (granules) Table 5 was mixed well, to which 20% of 100ml
エタノール水溶液に溶かしたB成分を練合させながら徐々に加え増粒した。 The B component was dissolved in aqueous ethanol was gradually added up grain while engaged kneaded. これを40℃で2昼夜送風乾燥させ、篩過、整粒し顆粒剤を得た。 This was two days air-dried at 40 ° C., to obtain sieving, the sizing and granules.

【0032】 [0032]

【表5】 [Table 5]

【0033】例8(注射剤) 表6の成分を溶解、濾過、滅菌し、アンプル中へ無菌充填し封入し、注射剤を得た。 [0033] Example 8 (Injection) dissolving the ingredients in Table 6, filtered, sterilized, aseptic filling and sealed into ampoules to obtain injections.

【0034】 [0034]

【表6】 [Table 6]

【0035】例9(化粧水) 表7の成分を室温で攪拌可溶化し、化粧水を得た。 [0035] The components of Example 9 (Lotion) Table 7 were stirred solubilization at room temperature to obtain a lotion.

【0036】 [0036]

【表7】 [Table 7]

【0037】例10(乳液) 表8のA、B、Cをそれぞれ80℃で加熱溶解し、AにBを加え、更にCを加え粗乳化し、ホモゲナイザーで均一に乳化し冷却して乳液を得た。 [0037] Example 10 (emulsion) Table 8 A, B, C were heated and dissolved at 80 ° C., respectively, the B added to A, further adding a crude emulsified C, and emulsion and homogeneously emulsified by a homogenizer cooled Obtained.

【0038】 [0038]

【表8】 [Table 8]

【0039】例11(ヘアトニック) 表9の成分を室温で攪拌可溶化し、ヘアトニックを得た。 [0039] Example 11 The components of (hair tonic) Table 9 and stirred solubilization at room temperature to obtain a hair tonic.

【0040】 [0040]

【表9】 [Table 9]

【0041】例12(シャンプー) 下記の表10に従ってシャンプーを作成した。 [0041] have created a shampoo according to Example 12 (shampoo) Table 10 below. 即ち、処方成分を秤込み加熱溶解させ、冷却してシャンプーを得た。 That is, the ingredients were weighed inclusive heated and dissolved, to obtain a shampoo and cooled.

【0042】 [0042]

【表10】 [Table 10]

【0043】例13(ジャム) 下記の処方成分を圧力鍋で30分煮た後、フードプロセッサーで均一に細切し、瓶に詰め封印し、滅菌処理してジャムを得た。 [0043] Example 13 (jam) after the ingredients below were boiled 30 minutes in a pressure cooker, uniformly minced with a food processor, and sealed bottled to obtain a jam by sterilization. リンゴ果肉 69重量部 サーチ果肉 10重量部 蔗糖 20重量部 ペクチン 1重量部 Apple pulp 69 parts by searching pulp 10 parts by weight of sucrose, 20 parts by weight of pectin and 1 part by weight

【0044】例14(果物ソース) 下記の処方成分を圧力鍋で30分煮た後、フードプロセッサーで均一に細切し、200#のストレイナーで漉して瓶に詰め封印し、滅菌処理し果物ソースを得た。 [0044] Example 14 after cooking 30 minutes (fruit source) pressure cooker the ingredients below were homogeneously minced with a food processor, 200 strained at # strainer sealed bottled, sterilized fruit to give a source. 苺果肉 50重量部 活性酸素消去剤1 10重量部 蔗糖 20重量部 水 19重量部 カルボキシメチルセルロース 1重量部 Strawberry pulp 50 parts by weight of the active oxygen eliminator 1 10 parts by weight of sucrose 20 parts by weight of water 19 parts by weight 1 part by weight of carboxymethyl cellulose

【0045】 [0045]

【実施例】以下に実施例を挙げて更に詳しく本発明について説明するが、本発明がこれら実施例に何等限定を受けないことは言うまでもない。 EXAMPLES will be described in more detail the present invention referring to Examples, but the present invention does not undergo any way limited to these examples.

【0046】実施例1 急性毒性試験(腹腔内投与) 1群5匹のICR雄性マウス(体重25〜30g)の腹腔内に、例1で得られた活性酸素消去剤1〜3を生理食塩水で溶解したものを1000mg/Kgの割合で投与した。 [0046] Example 1 Acute Toxicity Test intraperitoneally (i.p.) 1 group 5 mice ICR male mice (weighing 25-30 g), saline active oxygen eliminator 1-3 obtained in Example 1 those dissolved was administered at a rate of 1000 mg / Kg in. 投与後14日に生死を判定したが何れの群に於いても死亡例を認めなかった。 Was determined death in 14 days after administration but also in the one group of was no deaths. 従って、腹腔内投与によるLD50値は1000mg/Kgより大きいものと推定される。 Thus, LD50 values ​​by intraperitoneal administration is estimated to be greater than 1000 mg / Kg. 従って本発明の活性酸素消去剤は何れも安全性に優れていることが判る。 Thus active oxygen eliminator of the present invention is seen to have both excellent safety.

【0047】実施例2 急性毒性試験(経口投与) 1群6匹のICR雄性マウス(体重25〜30g)の経口投与で、例1で得られた活性酸素消去剤1〜3を生理食塩水で溶解したものを1000mg/Kgの割合で投与した。 [0047] In oral administration of Example 2 Acute toxicity test (oral administration) 1 group 6 mice ICR male mice (weighing 25-30 g), the active oxygen eliminator 1-3 obtained in Example 1 in physiological saline those dissolved was administered at a rate of 1000 mg / Kg. 投与後14日に生死を判定したが何れの群に於いても死亡例を認めなかった。 Was determined death in 14 days after administration but also in the one group of was no deaths. 従って、経口投与によるLD50値は1000mg/Kgより大きいものと推定される。 Thus, LD50 values ​​by oral administration is estimated to be greater than 1000 mg / Kg. 従って、本発明の活性酸素消去剤は何れも安全性に優れていることが判る。 Thus, active oxygen eliminator of the present invention is seen to have both excellent safety.

【0048】実施例3 活性酸素消去作用の測定(イン・ビトロ) 化1に示す反応式に基づき、キサンチン−キサンチンオキシダーゼ(XOD)系により活性酸素の一つであるスーパーオキシドアニオン(O 2 - )を発生させ、発生したO 2 -の生成率を亜硝酸法により測定し、この値をキサンチンオキシダーゼ阻害率値で補正して活性酸素消去作用値を求めた。 [0048] Example 3 Measurement of active oxygen scavenging action, based on the reaction formula shown in (in vitro) of 1, xanthine - superoxide anions which is one of active oxygen by xanthine oxidase (XOD) system (O 2 -) is generated, the generated O 2 - production rate of the measured by nitrous acid method to determine the active oxygen scavenging action value by correcting the value with xanthine oxidase inhibition percentage value.

【0049】 [0049]

【化1】 [Formula 1]

【0050】上記例1で得られた本発明の活性酸素消去剤1〜3を各種の濃度で含有する活性酸素消去剤水溶液0.1mlを、65mM燐酸2水素カリウム、35mM The active oxygen scavenger solution 0.1 ml, potassium 65mM phosphate 2 hydrogen active oxygen eliminator 1-3 containing at various concentrations of the present invention obtained in the above Example 1, 35 mM
ホウ酸ナトリウム、0.5mMEDTA2ナトリウム水溶液(以下、緩衝液Aと言う)0.2ml、0.5mM Sodium borate, 0.5MMEDTA2 aqueous solution (hereinafter, referred to as buffer A) 0.2 ml, 0.5 mM
キサンチン溶液0.2ml、10mMヒドロキシルアミン塩酸塩水溶液0.1ml、純水0.2mlの混合液に、加えてよく攪拌し試験液とした。 Xanthine solution 0.2 ml, 10 mM hydroxylamine hydrochloride solution 0.1 ml, in a mixture of pure water 0.2 ml, was added stirring well test solution. 同様にして、活性酸素消去剤の代わりに純水0.1mlを用いたコントロールの溶液を作成した。 Similarly, to create a solution of control using pure water 0.1ml instead of the active oxygen eliminator.

【0051】上記試験液及びコントロール溶液に、キサンチンオキシダーゼを1μl/ml濃度で含有する緩衝液Aを0.2ml加えて攪はんした後、37℃で30分インキュベーションした。 The above test solution and control solution, was 攪 solder xanthine oxidase added 0.2ml of buffer A containing at 1 [mu] l / ml concentration and incubated for 30 minutes at 37 ° C.. ブランクとして、上記と同様に調整された試験液及びコントロール溶液に、キサンチンオキシダーゼを含まない緩衝液Aを0.2ml加え、 As a blank, the test solution and control solution are adjusted in the same manner as described above, a buffer A without xanthine oxidase added 0.2 ml,
上記と同様に処理した溶液を用意した。 Was prepared solution was treated in the same manner as described above.

【0052】この様にして得られた各溶液のそれぞれに、30μMのN−1−ナフチルエチレンジアミン塩酸塩、3mMのスルファニル酸、25%氷酢酸混液2ml [0052] each of the thus-obtained solution, N-1-naphthyl ethylenediamine hydrochloride, 3 mM sulfanilic acid 30 [mu] M, 25% glacial acetic acid mixture 2ml
を加え、30分間室温で放置した後、各溶液について5 After the mixture was allowed to stand at room temperature for 30 minutes, 5 for each solution
50nmの吸光度で活性酸素の発生量を、295nmの吸光度で尿酸の発生量を測定した。 The generation quantity of active oxygen in 50nm absorbance was measured generation amount of uric acid in the absorbance of 295 nm.

【0053】得られた値を用いて、以下の式に基づき、 [0053] Using the obtained values, based on the following equation,
活性酸素消去活性値を算出した。 It was calculated active oxygen scavenging activity value. この値を非線形最小自乗法プログラムにかけ、IC50値を算出した。 Multiplying this value to a non-linear least squares method program, to calculate the IC50 value. 結果は表12に示す。 The results are shown in Table 12. これより、本発明の活性酸素消去剤が優れた活性酸素消去活性を有していることが判る。 From this, it can be seen that the active oxygen scavenging agents of the present invention has an excellent active oxygen scavenging activity.

【0054】(活性酸素消去活性を求める式) 活性酸素発生率=[(A 550-3 −A 550-4 )/(A 550-1 −A 550-2 )]*100 尿素生成率=[(A 295-3 −A 295-4 )/(A 295-1 −A 295-2 )]*100 活性酸素消去活性=100ー(活性酸素発生率/尿酸生成率)*100 但し、式中の記号は、表11に示す条件で調整された各溶液の吸光度の値とする。 [0054] (Request active oxygen scavenging activity formula) an active oxygen generation rate = [(A 550-3 -A 550-4) / (A 550-1 -A 550-2)] * 100 urea production rate = [( a 295-3 -A 295-4) / (a 295-1 -A 295-2)] * 100 active oxygen scavenging activity = 100-200 (active oxygen generation rate / uric acid production rate) * 100 where the symbols in formula is the value of the absorbance of each solution was adjusted under the conditions shown in Table 11.

【0055】 [0055]

【表11】 [Table 11]

【0056】 [0056]

【表12】 [Table 12]

【0057】 [0057]

【発明の効果】本発明の活性酸素消去剤は活性酸素消去作用に優れる上、安全性も高い。 Active oxygen eliminator of the present invention exhibits on excellent active oxygen scavenging action, also high safety. 従って、これを配合した、医薬品、食品、或いは飲料等の組成物は活性酸素が関与する疾患の予防と治療に大変有益である。 Thus was formulated the same, pharmaceutical, food, or compositions such as beverages are very useful in the prevention and treatment of diseases involving active oxygen.

フロントページの続き (51)Int.Cl. 6識別記号 庁内整理番号 FI 技術表示箇所 A61K 35/78 ACS A61K 35/78 ACS AGZ AGZ A23L 1/30 A23L 1/30 B A61K 7/00 A61K 7/00 K W 7/48 7/48 // A21D 2/36 A21D 2/36 A23G 3/00 101 A23G 3/00 101 A23L 1/06 A23L 1/06 2/38 2/38 C A61K 7/06 A61K 7/06 9/08 9/08 F Front page continued (51) Int.Cl. 6 in identification symbol Agency Docket No. FI art display portion A61K 35/78 ACS A61K 35/78 ACS AGZ AGZ A23L 1/30 A23L 1/30 B A61K 7/00 A61K 7 / 00 K W 7/48 7/48 // A21D 2/36 A21D 2/36 A23G 3/00 101 A23G 3/00 101 A23L 1/06 A23L 1/06 2/38 2/38 C A61K 7/06 A61K 7 / 06 9/08 9/08 F

Claims (7)

    【特許請求の範囲】 [The claims]
  1. 【請求項1】 グミ科ヒッポファエ属(Hippophae)の植物体のエッセンスからなる活性酸素消去剤。 1. A active oxygen eliminator consisting essence of plants elaeagnaceae Hippofae genus (Hippophae).
  2. 【請求項2】 グミ科ヒッポファエ属の植物がサーチである、請求項1記載の活性酸素消去剤。 Wherein elaeagnaceae Hippofae genus plant is searching, active oxygen scavenger of claim 1.
  3. 【請求項3】 植物体が実であることを特徴とする、請求項1又は2記載の活性酸素消去剤。 Wherein wherein the plant is a fruit, according to claim 1 or 2 active oxygen eliminator according.
  4. 【請求項4】 請求項1〜3の何れか一項に記載の活性酸素消去剤を含有する組成物。 4. A composition containing active oxygen scavenger according to any one of claims 1 to 3.
  5. 【請求項5】 用途が化粧料である、請求項4記載の組成物。 5. is a applications cosmetic composition of claim 4.
  6. 【請求項6】 用途が医薬品である、請求項4記載の組成物。 6. is a applications pharmaceutical composition of claim 4,.
  7. 【請求項7】 用途が食品である、請求項4記載の組成物。 7. is a purpose food composition of claim 4,.
JP3730796A 1996-01-31 1996-01-31 Active oxygen-eliminator and composition containing the same Pending JPH09208484A (en)

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WO2000053152A1 (en) * 1999-03-12 2000-09-14 International Cosmetics Corp. A composition including sea buckthorn oil extract and antioxidant and/or a uv filter
JP2005008539A (en) * 2003-06-17 2005-01-13 Fancl Corp Matrix metalloproteinase inhibitor
JP2005022993A (en) * 2003-06-30 2005-01-27 Kyoei Kagaku Kogyo Kk Agent for promoting production of collagen and elastin and ageing-resistant cosmetic containing the same
JP2006188488A (en) * 2004-12-06 2006-07-20 Kose Corp Anti-dermatopathy agent and skin care preparation for external use comprising the same
JP2007505042A (en) * 2003-09-08 2007-03-08 ジェニオウス バイオメド インターナショナル インコーポレイテッド The composition of botanical extracts for the treatment of cancer
US8137714B2 (en) 2008-07-25 2012-03-20 Mary Kay Inc. Compositions comprising docynia delavajy extract and/or Elaeagnus lancelotus extract
KR101237316B1 (en) * 2004-05-21 2013-02-28 타다시 고이노 Scalp and hair-care composition
JP2013522190A (en) * 2010-03-08 2013-06-13 イーエルシー マネージメント エルエルシー Compositions and methods for application to the skin
KR101276141B1 (en) * 2011-06-13 2013-06-18 동국대학교 산학협력단 Seed extract of seabuckthorn and method for producing the same

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US6187297B1 (en) * 1999-03-12 2001-02-13 Altai Cosmetics Ltd. Composition including sea buckthorn oil extract and antioxidant and / or a UV filter
US6524561B2 (en) * 1999-03-12 2003-02-25 Altai Cosmetics, Ltd. Composition including sea buckthorn oil extract and antioxidant and/or a UV filter
WO2000053152A1 (en) * 1999-03-12 2000-09-14 International Cosmetics Corp. A composition including sea buckthorn oil extract and antioxidant and/or a uv filter
JP2005008539A (en) * 2003-06-17 2005-01-13 Fancl Corp Matrix metalloproteinase inhibitor
JP2005022993A (en) * 2003-06-30 2005-01-27 Kyoei Kagaku Kogyo Kk Agent for promoting production of collagen and elastin and ageing-resistant cosmetic containing the same
JP4495925B2 (en) * 2003-06-30 2010-07-07 共栄化学工業株式会社 Collagen and elastin production-enhancing agents, as well as the anti-aging cosmetic composition containing the same
JP2007505042A (en) * 2003-09-08 2007-03-08 ジェニオウス バイオメド インターナショナル インコーポレイテッド The composition of botanical extracts for the treatment of cancer
US8173177B2 (en) 2003-09-08 2012-05-08 Genyous Biomed International Inc. Compositions of botanical extracts for cancer therapy
KR101237316B1 (en) * 2004-05-21 2013-02-28 타다시 고이노 Scalp and hair-care composition
JP2006188488A (en) * 2004-12-06 2006-07-20 Kose Corp Anti-dermatopathy agent and skin care preparation for external use comprising the same
US8137714B2 (en) 2008-07-25 2012-03-20 Mary Kay Inc. Compositions comprising docynia delavajy extract and/or Elaeagnus lancelotus extract
US9498427B2 (en) 2008-07-25 2016-11-22 Mary Kay Inc. Compositions comprising Elaeagnus lancelotus extract
US9040104B2 (en) 2008-07-25 2015-05-26 Mary Kay Inc. Compositions comprising Docynia delavajy extract and/or Elaeagnus lancelotus extract
US9808417B2 (en) 2008-07-25 2017-11-07 Mary Kay Inc. Compositions comprising Docynia delavajy extract and/or Elaeagnus lancelotus extract
JP2013522190A (en) * 2010-03-08 2013-06-13 イーエルシー マネージメント エルエルシー Compositions and methods for application to the skin
KR101276141B1 (en) * 2011-06-13 2013-06-18 동국대학교 산학협력단 Seed extract of seabuckthorn and method for producing the same

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