JPH0820544A - Reactive oxygen eliminator and food products and cosmetics formulated therewith - Google Patents

Abstract

PURPOSE:To obtain an active oxygen eliminator which comprises an extract from HANTAIKAI (dried mature seeds of a plant: Sterculia scaphigera, called 'ANNANNSHI', too), thus effectively eliminates active oxygen species (hydroxy radical, superoxide anion, hydrogen peroxide or the like) safely with reduced, side-effect and can be formulated into food products and cosmetics. CONSTITUTION:HANTAIKAI is extracted with water or an aqueous organic solvent (ethanol) and the extract is used as an active ingredient. It has very high action to eliminate active oxygen species and to suppress the lipid peroxidation and is useful in amelioration and prevention for a variety of disorders and troubles in beauty caused by increase in active oxygen species and peroxidized lipids in vivo. The suitable dose is 500-1,000mg/day/adult in the case of oral administration and 100-500mg/day/adult in the parenteral case. It can be formulated in seasonings, food products such as a seasoned food product, sweets, cosmetics such as solutions, creams, finish cosmetics, hair cosmetics or bathing agents.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an active oxygen scavenger containing an extract obtained by extracting Hantaikai with water or a water-containing organic solvent, and foods and cosmetics containing the active oxygen scavenger.

[0002]

The Background of the active oxygen, oxygen in the energy state rich in reactivity, specifically, OH · (hydroxyl radical), O ₂ ^- (superoxide anions), H ₂
It refers to O ₂ (hydrogen peroxide) and the like, which are reported to be involved in various reactions in the living body and affect diseases and aging.

For example, when a bacterium or virus invades the living body, neutrophils, monocytes or macrophages, which are phagocytes, migrate to the local area, initiate phagocytosis against the invading bacterium or virus, and continue to O ₂ ^- produces, the O ₂ ^- is sterilization,
It acts as a virus-killing and infected cell and acts as a self-defense of the living body. However, the excessive presence of this O ₂ ⁻ causes damage to normal cells and tissues.

Since the skin is the outermost layer of the body,
It is easily affected by active oxygen due to external factors such as ultraviolet rays and radiation.If this active oxygen is excessively present in the skin, radical peroxide produces lipid peroxide, which may cause abnormal formation of pigments on the skin such as spots and freckles. To be enhanced. O ₂ ⁻ generated by various causes in vivo is converted into H ₂ O ₂ by superoxide dismutase (hereinafter abbreviated as “SOD”).
Is converted to further H ₂ O ₂ is decomposed into water and oxygen by the action of the enzyme catalase and glutathione peroxidase. In addition to these enzymes, in vivo, vitamin C
There are antioxidants derived from foods such as vitamin E and vitamin E, which form a network that suppresses radical reactions and oxidation reactions of the living body, and protects against damage caused by active oxygen.

However, if the network is out of balance due to changes in the environment, such as infection with bacteria or viruses, changes in food intake or nutritional status, excessive irradiation with ultraviolet rays, and aging, the activity in the living body is lost. The amount of oxygen increases and, in addition, the amount of lipid peroxide increases. This allows
It is known that diseases such as rheumatoid arthritis, arteriosclerosis, diabetes, hepatitis, nephritis and the like; and dermatitis, spots, wrinkles, eczema, freckles, and other cosmetic disorders are caused.

[0006] In recent years, in order to improve and prevent the above-mentioned various diseases, a substance having an active oxygen scavenging effect and a lipid peroxide production suppressing effect is treated as a natural product from the viewpoint of pharmaceutical, food science or cosmetics science. The screening work from fermentation products or chemical products has been actively carried out, and as a result, a considerable number of active oxygen scavengers and lipid peroxide production inhibitors have become known.

For example, Japanese Patent Publication No. 4-34969 discloses an active oxygen scavenging and removing agent containing baicalein, which is a flavonoid-based component contained in Aogon.
In Japanese Patent No. 27938, an active oxygen scavenger composed of clove oil or dehydrodioigenol is disclosed in JP-A-5-43.
No. 463 discloses 1,3-dimethyluric acid or 1,3.
Hydrogen peroxide and a superoxide (superoxide) scavenger containing 7-trimethyluric acid as an active ingredient, and an active oxygen scavenger / scavenger containing an Aspalathus linealis extract as an active ingredient in JP-A-5-271063,
Japanese Patent Application Laid-Open No. 6-40909 discloses SOD containing 4 ', 7,8-trihydroxyisoflavone as an active ingredient.
As such an activator, JP-A-4-327534 proposes a prophylactic and therapeutic agent for disorders caused by active oxygen containing iron chlorophyllin as an active ingredient.

[0008] As the lipid peroxide production inhibitor, those containing a reaction product of a lactic acid bacterium fermentation product as an active ingredient in JP-A-5-9126 are effective as sesamin and / or episesamin in JP-A-5-51388. The ingredients have been proposed. Furthermore, foods and cosmetics containing a component having an active oxygen scavenging effect have also been proposed, and in JP-A-5-32556, a skin external preparation obtained by further adding SOD to a Ginkgo biloba extract having an active oxygen scavenging effect,
Japanese Unexamined Patent Publication (Kokai) No. 5-316963 proposes a superoxide scavenger containing, as an active ingredient, an extract of V. oak and / or white oak, and foods and cosmetics containing the same.

[0009]

As described above, various compositions having active oxygen scavenging ability have been proposed. Among them, there are problems such as side effects such as sensitization. Some of them have problems, and few are in practical use. Accordingly, the present invention provides an active oxygen scavenger that effectively eliminates active oxygen and has high safety with few side effects on the human body, and foods and cosmetics containing the active oxygen scavenger. And

[0010]

Means for Solving the Problems As a result of intensive studies to solve the above problems, the present inventors have extracted Hantaykai, which is conventionally known as a crude drug or food and has a high safety to human body, with water or a water-containing organic solvent. The present inventors have completed the present invention by finding that the extract thus obtained has a strong effect of eliminating active oxygen.

That is, the present invention provides an active oxygen scavenger containing an extract obtained by extracting Hantaikai with water or a water-containing organic solvent as an active ingredient, and foods and cosmetics containing the active oxygen scavenger. It is a thing.
Hereinafter, the present invention will be described in detail.

[0012] Hantaykai is a plant of the family Aequiliidae Sterculia sca, which is distributed from southern China to Southeast Asia.
Dried mature seeds of phigera, also known as "Annanko"
It has also been used as a crude drug or food that is expected to have a cathartic or antihypertensive effect in China. In Japan, it is also called "Bakudai", and the sponge-like flesh that is rehydrated in water is used as sashimi for edible purposes.

The active oxygen scavenger of the present invention contains, as an active ingredient, an extract obtained by extracting the above Hantaikai with water or a water-containing organic solvent. The extraction method is not particularly limited and may be performed according to a conventional method. Hereinafter, a preferable extraction method will be described. As the Hantaykai to be used, a commercially available product can be used as it is, but in order to perform extraction efficiently, it is preferable to cut or grind the Hantaykai before use.

Further, as the extraction solvent, only water can be used, but in this case, a water-containing organic solvent is preferably used because the Hantaikai swells like a sponge and becomes slightly difficult to handle. Examples of the organic solvent preferably used include hydrophilic organic solvents such as methyl alcohol, ethyl alcohol and acetone. The mixing ratio of water and the organic solvent is not particularly limited, but preferably,
A water content of about 30 to 50% by weight relative to the total solvent is used.

The extract obtained by extracting only with an organic solvent such as ethyl alcohol or acetone was a green oily substance and had no active oxygen scavenging effect. The amount of the extraction solvent to be used is not particularly limited and may be about 2 to 100 times the weight of the Hantaikai to be used, but when only water is used, the spongy Hantaikai is removed from the solvent. For complete separation, a slightly higher amount is used, preferably about 200 times the weight of Hantaykai.

The extraction temperature and extraction time are not particularly limited, but extraction is preferably performed at room temperature, particularly preferably at a temperature of about 20 to 30 ° C. for about 24 to 72 hours. When only water is used as the extraction solvent, the extraction solvent may be heated to shorten the extraction time,
If it is heated to, the extraction time can be about 5 minutes.

Particularly preferably, as described above, extraction with an organic solvent such as ethyl alcohol is carried out before extraction with water or a water-containing organic solvent to obtain a green oily substance (inactive portion) having no active oxygen scavenging effect. except for. In this case, an appropriate amount of an organic solvent such as ethyl alcohol is added to the ground product of Hantaykai, preferably at room temperature, particularly preferably about 20 to 30 ° C.
Extraction is carried out at a temperature of about 12 to 60 hours, the soluble part is removed by means such as filter paper, natural filtration with absorbent cotton, vacuum filtration and the like, and then water or a water-containing organic solvent is added to the obtained insoluble part, Extract.

After extraction, a water-containing organic solvent-soluble portion is obtained by means such as natural filtration using filter paper or absorbent cotton, vacuum filtration or the like, and then the solvent is distilled off from the water-containing organic solvent-soluble portion by means of vacuum concentration or the like. Further, if necessary, freeze-drying or the like is carried out to obtain the active ingredient of the present invention. The active ingredient of the present invention thus obtained has an extremely high active oxygen scavenging effect and lipid peroxidation inhibitory effect, and is extremely safe, and even if a processing means such as heating is added, the effect is lost. Since it is stable without being exposed, it is possible to obtain a very useful active oxygen scavenger by incorporating this active ingredient.

The active oxygen scavenger of the present invention is orally administered by tablets, capsules, powders, granules, liquids, etc .; subcutaneous, intramuscular or intravenous injections; mixed infusion administration; rectal administration by suppositories, ointments, lotions. It can be administered by any method such as topical application with a drug or the like. It is possible to administer the active ingredient as an active oxygen scavenger as it is, but by a known method, it is mixed with an appropriate base,
It is also possible to formulate.

For example, in the case of tablets, capsules, powders or granules for oral use, the above-mentioned active ingredient is an excipient such as starch, lactose or mannitol; a binder such as carboxymethylcellulose or hydroxypropylcellulose; crystalline cellulose, It can be produced by appropriately combining and precipitating a disintegrating agent such as carboxymethyl cellulose calcium; a lubricant such as talc and magnesium stearate; and a wetting agent, a coloring agent, a perfume and the like, if necessary.

In the case of oral liquid preparations, an aqueous or oily emulsion or syrup may be used, and a suspending agent such as simple syrup, sorbitol, methylcellulose, sodium carboxymethylcellulose; egg yolk lecithin, sorbitan monofatty acid ester, Emulsifiers such as lauromacrogol and castor oil; other preservatives, preservatives, stabilizers and the like can be appropriately added as required.

In the case of an injection, it may be in the form of an oil solution, an emulsion or an aqueous solution, and these solvents may be emulsifiers commonly used such as polyoxyethylene hydrogenated castor oil and methyl paraoxybenzoate. Preservatives and the like can be added as appropriate. In the case of suppositories, hydrophobic bases such as cacao butter and lanolin fat; hydrophilic bases such as macrogol and glycerin; emulsifiers such as lecithin can be appropriately mixed.

In the case of an ointment, a hydrophobic base such as petrolatum, paraffin, silicone, plastibase, vegetable oil and waxes; a hydrophilic base such as purified lanolin, carboxyvinyl polymer and propylene glycol; an emulsifier such as triethanolamine. Can be appropriately mixed. Although the dose of the active oxygen scavenger of the present invention varies depending on the degree of disease, it is usually about 500 to 500 by oral administration in adults.
1000 mg, about 100-500 mg for parenteral administration
Is preferably administered once to several times a day.

The active oxygen scavenger of the present invention as described above is
It has an extremely high active oxygen scavenging effect and lipid peroxidation inhibitory effect, and is extremely safe, and causes various diseases and cosmetic disorders caused by an increase in the amount of active oxygen and lipid peroxide in the living body. It is useful for improving and preventing. The active enzyme scavenger of the present invention can be composed of an active ingredient itself or a formulated one, and can be added to ordinary foods or cosmetics to impart an active oxygen scavenging effect and enhance its commercial value.

Various forms of the food of the present invention can be mentioned, for example, miso, soy sauce, noodle soup,
In addition to being mixed with a raw material for seasonings such as mayonnaise, it can be added to various cooked foods; desserts, frozen desserts, confectioneries such as candy and chewing gum, and beverages. Any ingredients depending on the purpose of use can be used in these foods. For example, in the case of frozen desserts and beverages, fruit juice, sweeteners, acidulants, colorants, flavors and the like can be appropriately added, and in the case of candy, in addition to a base such as sucrose, starch syrup, and water, sourness A coloring agent, a coloring agent, a fragrance and the like can be appropriately mixed according to a conventional method. Other products can also be appropriately blended with components according to their types.

The amount of the active ingredient of the present invention added to food may be appropriately changed depending on its form. For example, in the case of seasonings such as miso, mentsuyu and mayonnaise, the total amount is about 0.3 to.
It is preferably 3% by weight, and in the case of other foods, it is preferably about 0.1 to 1% by weight. In order to ingest the food of the present invention and to expect its effect, the amount of the active ingredient in an adult is usually about 500 to 1000 m per day.
It is preferable to be g, usually 6 per day
About 00 mg is sufficient.

Further, as the form of the cosmetic of the present invention, various lotions such as astringent lotion; various lotions such as after-shave lotion; milky lotion; various creams such as massage cream and nutrition cream; eye packs, Various packs such as paste wash-off packs; Finishing cosmetics such as white powder, foundation, lip balm, lipsticks; Hair cosmetics such as hair tonics, pomades, shampoos, rinses, treatments, hair creams; Facial cleansers Other cleaning agents; bath agents;
Examples include antiperspirants and deodorants.

[0028] These cosmetics may contain any ingredients depending on the purpose of use. For example, in the case of cream, hydrophobic bases such as petrolatum, paraffin, liquid paraffin, squalane; lanolin and propylene glycol. Hydrophilic bases such as: emulsifiers such as fatty acid monoglycerides and sorbitan fatty acid esters; preservatives such as methyl paraoxybenzoate; pigments; fragrances; and other nutrients, moisturizers, UV inhibitors, etc. It can be appropriately mixed according to the method. Similarly, for other products, components depending on the type can be appropriately blended.

The amount of the active ingredient of the present invention added to cosmetics is
It may be appropriately changed depending on its form (for example, a large amount is added in the case of a bath agent), but it is preferably about 0.1 to 3% by weight of the whole cosmetic. As described above, the food and cosmetics of the present invention containing an active oxygen scavenger have various illnesses and various cosmetic disorders that are caused by an increase in the amount of active oxygen and lipid peroxide in the living body. , It is useful to prevent from day to day.

Next, production examples of the active ingredient of the present invention, effect test examples of the active ingredient, and safety test examples of the active ingredient will be described.

[Production Example] Hantaykai crushed product 790 g
Ethyl alcohol (2 liters) was added to the mixture, and the mixture was extracted at room temperature for 48 hours and filtered with a filter paper to remove the ethyl alcohol-soluble portion (green oily substance, weight after concentration and drying, 33 g). 2 parts of 50 wt% hydrous ethyl alcohol was added to the mixture and the mixture was extracted at room temperature for 48 hours. After filtering the hydrous ethyl alcohol soluble part with filter paper, 40 ℃
The solution was concentrated under reduced pressure to remove water and ethyl alcohol, and then freeze-dried to obtain the desired active ingredient (brown powder, yield 44 g, yield 5.6%).

No active oxygen scavenging effect was observed in the ethyl alcohol-soluble portion removed in the production process of this production example. In addition, an active oxygen scavenging effect was observed for the component obtained by adding an appropriate amount of water to the obtained active ingredient, performing heat treatment at 120 ° C. for 15 minutes, and then dehydrating. That is, it was confirmed that the active ingredient of the present invention is stable without losing the active oxygen scavenging effect even when heated.

The infrared spectrum of the obtained active ingredient was as follows. The measuring device is made by JASCO Corporation I
R-810 type, the sample preparation method was performed by the KBr method. 3400-3300 (broad), 2925, 1615, 1525, 1400, 1050 cm
^-1

[0034] [Effect Test Example 1 O ₂ ^- O ₂ active ingredients erase test the present invention ^- the erasing effect, C.Beauchamp et al. Method (Analytical Biochemistry, vol.44, pp.276-287
(1971)) was examined by a partially modified nitroblue tetrazolium (NBT) reduction method. This method utilizes that O ₂ ⁻ generated when xanthine oxidase (XOD) is allowed to act on hypoxanthine (HPX) reduces NBT to produce black blue insoluble formazan granules. The system in O ₂ ^- When a substance having an erase effect (sample = active ingredient of the present invention) is present, since the amount of formazan generated by the reduced relative to formazan product amount when the active ingredient without additives, formazan when the active ingredient added from the amount to obtain the formazan product inhibition rate, O ₂ of the active ingredient of the present invention ^- was erasure rate.

Further, in this method, if it has the effect of analyte to inhibit the enzymatic action of XOD is, O ₂ ^- even if no erasing effect, O ₂ ^- so becomes high on the erasure rate apparently, active ingredient of the present invention, rather than XOD inhibitory effect, O ₂ ^- to confirm that it has an erase effect, X
The OD inhibition rate was also examined.

[0036] <O ₂ ^- method of measuring the erasing ratio> 50 mM (millimolar, hereinafter the same) sodium carbonate buffer (pH 1
0.2) 2.4 ml, 3mM HPX aqueous solution 0.1m
1, 0.75 mM NBT 0.1 ml, 3 mM EDTA
0.1 ml of an aqueous solution of (ethylenediaminetetraacetic acid) and 0.1 ml of 0.15% bovine serum albumin contained 0.3% by weight of a sample solution (the active ingredient of the present invention obtained in Production Example 5% by weight).
A 0 wt% water-containing ethyl alcohol solution) was added so that the final concentration of the active ingredient in the reaction liquid would be the concentration shown in Table 1 below, and the mixture was placed in a cuvette (absorption cell) and purified with purified water (0.05 U). 0.1 ml of diluted XOD was added to the enzyme unit, and the absorbance (A) at 560 nm after 5 minutes from the reaction start was measured by a spectrophotometer (UVDEC manufactured by JASCO Corporation).
430B) to determine the amount of formazan produced. As a control, the absorbance (B) was similarly measured using a mixed solution containing the same amount of purified water instead of the sample solution. further,
As a blank of the sample mixture, the absorbance (C) was similarly measured for the reaction mixture obtained by adding the heat-inactivated XOD to the sample mixture, and the following formula 1:

[0037]

[Equation 1] More of the active ingredient of the present invention O ₂ ^- was calculated erasure rate.

[0038] <XOD inhibitory rate measurement method> The O ₂ ^- purified water instead of 0.75 mM NBT in the erasing ratio measurement method
Using a mixed solution containing 0.1 ml, X
The absorbance (X) at 290 nm after 5 minutes from the addition of OD was measured to determine the amount of uric acid produced due to the oxidation of HPX by XOD. As a control, the absorbance (Y) of a mixed solution in which the same amount of purified water was added instead of the sample solution was measured. Further, as a blank of the sample mixture, the absorbance (Z) was similarly measured for the reaction mixture obtained by adding the heat-inactivated XOD to the sample mixture, and the following formula 2:

[0039]

[Equation 2] The inhibition rate of XOD of the active ingredient of the present invention was calculated from the above.

<Results> The above results are shown in Table 1.

[0041]

[Table 1] As can be seen from Table 1, the active ingredient of the present invention is 100 p
without substantially inhibiting the enzymatic action of XOD in the following concentrations pm, very high O ₂ ^- it is clear that represents erasing effect.

[Effect test example 2] OH / elimination test The OH / elimination effect of the active ingredient of the present invention was evaluated by the deoxyribose method (Analytical Biochemistry, vo) of B. Halliwell et al.
l.165, pp.215-219 (1987)) was partially modified. This method uses OH generated from Fenton's reagent (oxidative coloring reagent that is a mixture of hydrogen peroxide and iron (II) salt).
· Malondialdehyde (MDA) produced by the reaction of thiobarbituric acid (TBA) with deoxyribose
A red reaction product is produced by reacting with, and the production amount is determined by measuring the absorbance (TBA value) at 532 nm. The presence of a substance having an OH / elimination effect in this system (specimen = active ingredient of the present invention) reduces the amount of MDA produced and the TBA value, so that the TBA value when the active ingredient of the present invention is not added is reduced. The MDA production inhibition rate was determined from the TBA value when the active ingredient of the present invention was added, and was defined as the OH / elimination rate of the active ingredient of the present invention.

<Method of measuring OH / erase ratio> 200 mM
0.2 ml of KH ₂ PO ₄ -KOH buffer solution (pH 7.4),
28 mM deoxyribose aqueous solution 0.2 ml, 10 mM
H ₂ O ₂ 0.2 ml, 1 mM FeCl ₃ aqueous solution 0.2 m
1, 1 mM ascorbic acid aqueous solution 0.2 ml, 1.04
mM EDTA aqueous solution 0.2 ml, purified water 0.6 ml and sample solution (a solution obtained by diluting the active ingredient of the present invention obtained in Production Example with water so that the final concentration in the reaction solution is the concentration shown in Table 2 below). Mix the 0.2 ml mixture at 37 ° C for 1
After reacting for 20 hours, 20% trichloroacetic acid aqueous solution 2 m
1 and 1 ml of a 0.67% TBA aqueous solution were added, and the mixture was heated in a boiling water bath for 10 minutes. After cooling, the absorbance (E) at 532 nm was measured by a spectrophotometer (UV manufactured by JASCO Corporation, UV).
It measured by DEC430B) and calculated | required the TBA value. As a blank of the sample mixture, the absorbance (F) was similarly measured using a mixture containing purified water instead of 10 mM H ₂ O ₂ . Further, as a control, the absorbance (G) when purified water was added instead of the sample solution, and as a control blank, purified water was added instead of the sample solution, and 10
The absorbance (H) when purified water was added instead of mM H ₂ O ₂ was measured, and the following formula 3:

[0044]

(Equation 3) The OH / elimination rate of the active ingredient of the present invention was calculated from the above.

<Results> The above results are shown in Table 2.

[0046]

[Table 2] As can be seen from Table 2, it is clear that the active ingredient of the present invention exhibits a very high OH / erasing effect.

[Effect Test Example 3] Lipid Peroxidation Inhibition Test The lipid peroxidation inhibitory effect of the active ingredient of the present invention was measured by A. Jitoe.
Et al. (Journal of Agricultural and Food Chemistr
y, vol.40, pp.1337-1340 (1992)), and linoleic acid was used as the lipid, and the thiocyanate method was used for the investigation. This method utilizes the fact that ferrous ion is oxidized to ferric ion by the peroxide of linoleic acid, and the produced ferric ion is compared as a thiocyan complex salt.

<Measurement Method of Lipid Peroxidation Inhibition Rate> 99.5
Wt% ethyl alcohol 4 ml, 2.53 wt% linoleic acid solution dissolved in 99.5 wt% ethyl alcohol
4.104 ml, 50 mM phosphate buffer (pH 7.0)
The reaction mixture obtained by adding 20 mg of the active ingredient of the present invention obtained in Preparation Example to 8 ml and distilled water (3.896 ml) was placed in a 50 ml capacity vial with a screw cap, and left in a thermostat at 40 ° C in the dark. did. A positive control was prepared by adding dibutylhydroxytoluene or α-tocopherol instead of the active ingredient of the present invention.

After 8 days, to 0.1 ml of each reaction solution, 9.7 ml of 75 wt% hydrous ethyl alcohol and 0.1 ml of 30 wt% ammonium thiocyanate aqueous solution were added. To this, 0.1 ml of a 0.02 M ferrous chloride solution dissolved in 3.5% hydrochloric acid water was added, and the absorbance (M) at 500 nm was measured exactly 3 minutes later. Further, as a control, the absorbance (N) in the case where the active ingredient of the present invention or the ingredient of the positive control was not added was measured, and the following formula 4:

[0050]

[Equation 4] The lipid peroxidation inhibition rate of each component was calculated from the above.

<Results> The above results are shown in Table 3.

[0052]

[Table 3] As can be seen from Table 3, it is clear that the active ingredient of the present invention exhibits a lipid peroxidation inhibitory effect.

[Safety Test Example 1] Acute Toxicity Test by Single Oral Administration 5 male Wistar rats having a body weight of 180 g were treated with 2 g / kg (body weight) of the active ingredient of the present invention obtained in the Production Example. When it was orally administered and then fed normally for 2 weeks, the mortality rate was 0%. Therefore, the half-lethal dose (LD ₅₀ ) of the active ingredient of the present invention was 2 g / kg or more, and the toxicity was determined to be extremely low.

[Safety Test Example 2] Mutagenicity Test Salmonella typhimurium TA 98, TA
100, TA 1535, TA 1537 and E. coli (Escherichia c
oli) WPuvrA was used to perform the Ames test. The active ingredient of the present invention obtained in Production Example was added to each petri dish so as to give a concentration of 200 μg to 5000 μg, and the test was conducted. As a result, all of the test bacteria grew regardless of the presence or absence of the metabolic activation system. Regarding the number of colonies, the number of revertant colonies of the negative control (without addition of the active ingredient of the present invention) did not exceed 2-fold, so that the mutagenicity of the active ingredient of the present invention was determined to be negative.

[Safety Test Example 3] Sensitization Test: Patch Test for Guinea Pigs Hartley female guinea pig 10 having a body weight of 330 to 400 g
When 20 μl of an acetone solution containing 40% by weight of the active ingredient of the present invention obtained in Production Example was applied to a rat and an Adjuvant patch test was performed, the positive rate was 0%, indicating that the present invention is effective. It was judged that the sensitizing property of the component was extremely low.

[Safety Test Example 4] Sensitization test: patch test for humans For 40 healthy men and women aged 20 to 50, the lanolin solution containing 5% by weight of the active ingredient of the present invention obtained in Production Example was used. When a patch test was carried out according to a conventional method, no irritation was observed at the application site in any of the 40 people, and the irritation to human skin was determined to be negative.

As a result of the above test, the active ingredient of the present invention was
It is clear that it has extremely high active oxygen scavenging effect and lipid peroxidation suppressing effect, and is extremely safe.

[0058]

[Examples] Examples of active oxygen scavengers containing the active ingredient of the present invention and foods and cosmetics containing the active ingredient of the present invention will be shown below, but the present invention is not limited to these examples. Not something.

Example 1 Tablet ────────────────────────────── Component Weight ────────── ────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────── Magnesium 2 mg ────────────────────────────── The above ingredients were mixed by a conventional method and the particle size was adjusted through a 60 mesh wire mesh. Then, one tablet was produced using a tableting machine.

Example 2 Capsule ────────────────────────────── Component Weight ───────── ───────────────────────────────────────────────────────────────────────────────────── ────────────────────── The above ingredients are thoroughly mixed by a conventional method, the particle size is adjusted through a 60-mesh wire mesh, and then gelatin capsules are filled. One agent was manufactured.

[Example 3] Powder ────────────────────────────── Component weight ────────── ──────────────────────────────────────────────────────────────── ───────────────── The above ingredients were mixed uniformly by a conventional method to produce a powder.

Example 4 Injection ────────────────────────────── Component Weight ───────── ──────────────────────────────────────────────────────────────────────────────────────────────────── ───────────────────────── The above ingredients are mixed according to the usual method, filtered, filled in a container and sealed, and then sterilized by high pressure steam. Then, 1 ampoule of the injection was prepared.

Example 5 Suppository ────────────────────────────── Component Weight ───────── ───────────────────────────────────────────────────────────────────────────── ──────────────── The above ingredients were well stirred at 50 ° C., homogenized, poured into a container, and cooled and solidified to produce one suppository.

Example 6 Gel Ointment ────────────────────────────── Component Blending Ratio (wt%) ── ──────────────────────────── Active ingredient of the present invention obtained in Production Example 0.05 Propylene glycol 10.0 Carboxyvinyl polymer 1 0.0 Triethanolamine 1.0 Perfume 0.05 Purified water 87.9 ─────────────────────────────── Gel ointment 1 that is uniformly mixed and dissolved by a conventional method
0 g was produced.

[Example 7] Miso ────────────────────────────── Ingredients Mixing ratio (% by weight) ──── ─────────────────────────────────────────────────────────────────────────────────────────────────── ──────────────────────── The above ingredients were mixed to produce 100 g of miso.

[Example 8] Mentsuyu ────────────────────────────── Ingredients Mixing ratio (% by weight) ──── ──────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────── Liquid 1.5 ──────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────.

Example 9 Mayonnaise ────────────────────────────── Ingredients Mixing Ratio (% by Weight) ──── ──────────────────────────────────────────────────────────────────── ─────────────────────── The above ingredients were mixed to produce 100 g of mayonnaise.

Example 10 Frozen dessert ────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────―― ────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────── ────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────── Was manufactured.

[Example 11] candy ────────────────────────────── Ingredients ratio (wt%) ──── ───────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────── 1.0 Perfume 0.2 ────────────────────────────── Add an appropriate amount of water to the above ingredients sucrose and starch syrup According to a conventional method, the candy was heated to 150 ° C. at a normal pressure, and the remaining components were added before cooling and solidification to produce one candy.

[Example 12] Chewing gum ────────────────────────────── Ingredient ratio (% by weight) ──── ────────────────────────── Active ingredient of the present invention obtained in Production Example 0.2 Gum base 35.0 Powdered sugar 42.0 Glucose 20 .0 Calcium carbonate 2.0 Fragrance 0.8 ────────────────────────────── The above ingredients are kneaded in a kneader by a conventional method. , One chewing gum was produced.

Example 13 Beverage ────────────────────────────── Ingredient Mixing Ratio (% by Weight) ──── ───────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────. .0 ascorbic acid 0.1 fragrance 0.1 mineral water 69.7 ─────────────────────────────── After stirring and mixing uniformly by the method, the filtered filtrate was bottled and sterilized to produce one bottle of beverage.

[Example 14] Lotion ────────────────────────────── Component blending ratio (% by weight) ─── ─────────────────────────── Active ingredient of the present invention obtained in Production Example 0.2 Ethyl alcohol 10.0 Propylene glycol 6.0 Glycerin 5.0 Polyoxyethylene sorbitan monolaurate (20 EO) 1.0 Methyl paraoxybenzoate 0.2 Perfume 0.2 Purified water 77.4 ──────────────── ─────────────── 100 g of lotion was prepared by uniformly mixing the above components by a conventional method.

Example 15 Emulsion ────────────────────────────── Ingredients Mixing ratio (% by weight) ──── ─────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────── Salix beeswax 0.5 Sorbitan sesquioleate 0.8 Polyoxyethylene oleyl ether (20 EO) 1.2 b Component: 2% xanthan gum aqueous solution 20.0 Propylene glycol 5.0 Ethyl alcohol 4.0 Methyl paraoxybenzoate 0.2 Purified water 60.9 Fragrance 0.2 ───────────────────────────────────────── , Component b is heated and dissolved at 73 ° C., and component a is added to component b with stirring to emulsify, The active ingredient of the present invention and the fragrance were added and mixed uniformly to prepare 100 g of an emulsion.

Example 16 Cream ────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────── ──────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────── to─ to to have been selected, and can be used for the following reasons. 0 Paraffin 7.0 Lanolin 4.0 Sorbitan sesquioleate 4.0 Component b: Propylene glycol 2.5 Magnesium sulfate 0.2 Methyl paraoxybenzoate 0.2 Purified water 31.7 Perfume 0.2 ───── ───────────────────────── The above components a and b are heated and melted at 73 ℃ and 75 ℃, respectively. The ingredients are gradually added with stirring to emulsify, and after cooling to 55 ° C, the active ingredient of the present invention and the fragrance are added. Then, 100 g of cream was prepared by uniformly mixing with a homogenizer.

[Example 17] Pack ────────────────────────────── Component blending ratio (% by weight) ──── ───────────────────────────────────────────────────────────────────────────────── ───────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────── 0 Propylene glycol 4.0 Purified water 66.4 b component: Ethyl alcohol 8.0 Polyoxyethylene oleyl ether (15 EO) 1.0 Methyl paraoxybenzoate 0.2 Perfume 0.2 ─────── ──────────────────────── After uniformly dissolving a component of the above components at room temperature, add polyvinyl alcohol gradually to disperse the mixture, It was heated to and dissolved. This was cooled, and the active ingredient of the present invention was added to the component b in advance to form a uniform phase, which was then mixed to prepare 100 g of a pack.

[Example 18] Cleaning agent ───────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────── ──────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────── Acid 10.0 Palmitic acid 8.0 Lauric acid 6.0 Oleyl alcohol 1.5 Purified lanolin 1.0 Potassium hydroxide 5.0 Methyl paraoxybenzoate 0.2 Perfume 0.2 Purified water 38.9 ──── ─────────────────────────────────────────────────────── ───────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────

[Example 19] Bath agent ────────────────────────────── Component blending ratio (% by weight) ─── ───────────────────────────────────────────────────────────────────────────────────────── ──────────────────────────── In Japanese) 0 Sodium chloride 14.0 Dye 1.0 Fragrance 1.0 ─────────────────────────────── 100 g of a bath agent was prepared by mixing.

[0078]

According to the present invention, extremely high active oxygen scavenging effect and lipid peroxidation suppressing effect are obtained, and
The safety is extremely high, and it is possible to improve and prevent various diseases caused by an increase in the amount of active oxygen and lipid peroxide in the living body and various symptoms which are cosmetic disorders.

Claims (3)

[Claims] 1. An active oxygen scavenger containing an extract obtained by extracting Hantaikai with water or a water-containing organic solvent as an active ingredient. 2. A food containing the active oxygen scavenger according to claim 1. 3. A cosmetic comprising the active oxygen scavenger according to claim 1.