JP2004323362A - Composition comprising extract from plant of genus bidens - Google Patents
Composition comprising extract from plant of genus bidens Download PDFInfo
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- JP2004323362A JP2004323362A JP2003115703A JP2003115703A JP2004323362A JP 2004323362 A JP2004323362 A JP 2004323362A JP 2003115703 A JP2003115703 A JP 2003115703A JP 2003115703 A JP2003115703 A JP 2003115703A JP 2004323362 A JP2004323362 A JP 2004323362A
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Abstract
Description
【０００１】
【発明の属する技術分野】
本発明はセンダングサ属植物、特にビデンス・ピローサ抽出物を含む組成物及び抽出物の製造方法に関する。
【０００２】
【従来の技術】
ビデンス・ピローサは昔から身近にあるハーブであり、民間薬草として干した地上部分を煎じ、解熱・解毒・消炎・鎮痛・止瀉・利尿薬として肝炎・腎炎・盲腸炎・糖尿病・膀胱炎・尿道炎・リウマチ性関節炎・気管支炎・腫れ物・胃腸病・下痢・消化不良などに内用し、外用では咽喉の腫れや痛み、打撲傷などに用いられてきた。特に、盲腸炎には癒着のある重症のものでも、煎じ汁を飲むか、新芽をそのまま食べ、或いは６０ｇほどを搗いて汁を飲む（蜜とか食塩を少々加えてもいい）と炎症も痛みも収まるとされている。また、腫れた咽喉の痛みには飲むと同時にうがい薬として使用することもよいとされている。また、多少の殺菌静菌作用もあるため打撲などの外傷や、腫れた所を洗ったり、局所に塗ったりなど外用薬としても使われてきた。
従来、ビデンス・ピローサの使用方法としては、内服用または外用として水で抽出したもの（煎じられたもの）が用いられてきた。例えば、生の植物１０〜１５ｇを水４００ｍＬで煎じて半量まで煮詰めて飲むなどの方法がある。なお、ビデンス・ピローサは台湾では家庭用民間薬として、日本におけるドクダミやゲンノショウコなどのように野生のものを摘んで使用する人も多い。また中医（漢方医）の使う薬屋では調剤用咸豊草抽出物粉末が入手できる。花は黄色もあるが薬用には白色が良いとされている。
ビデンス・ピローサの成分研究の目的では、水抽出またはメタノールやエタノール等の溶媒による抽出が行われている（例えば非特許文献１参照）が、ビデンス・ピローサに他の添加物を添加して抽出を行った例は知られていない。
【０００３】
【非特許文献１】
Ｐａｕｌ Ｖ． Ｔａｎ ｅｔ ａｌ．， Ｊｏｕｒｎａｌ ｏｆ Ｅｔｈｎｏｐｈａｒｍａｃｏｌｏｇｙ ７３， ４１５ （２０００）
【０００４】
【発明が解決しようとする課題】
本発明は、センダングサ属植物の有用性、有効性を更に高めるセンダングサ属植物の抽出物を製造する方法及び前記抽出物を含む組成物を提供することを目的とする。
【０００５】
【課題を解決するための手段】
本発明者はセンダングサ属植物につき種々検討を重ねた結果、ショウガまたはウコン等のショウガ科植物を予めセンダングサ属植物に混合して抽出した抽出物を含む組成物が、センダングサ属植物単独で抽出したものよりも高血糖、皮膚炎、花粉症等の治療及び予防において、また美肌剤や日焼け止め等の化粧品として優れた効果を示すことを発見した。また、センダングサ属植物とショウガ科植物を混合したものを抽出した抽出物の方がそれぞれ単独で抽出したものを混合するよりも優れた効果、すなわち相乗効果を示すことを見出した。
すなわち本発明は、センダングサ属植物にショウガ科植物を混合して抽出溶媒にて抽出することにより製造されたセンダングサ属植物抽出物を含む組成物を提供するものである。
本発明はまた、センダングサ属植物にショウガ科植物を混合して抽出溶媒により抽出することを特徴とするセンダングサ属植物抽出物の製造方法を提供するものである。
【０００６】
【発明の実施の形態】
本発明に使用されるセンダングサ属植物は、特開２００１−１７８３９１号公報および特開２００１−２３３４２７号公報に記載されるように、学名ではビデンス（Ｂｉｄｅｎｓ）属と言われる一群の植物である。種類も多岐に亘り互いに交配するので変種も多く、植物学上も混乱が見られ、学名、和名、漢名、の対応も交錯していて同定することは極めて困難であるが、本発明で用いられるセンダングサ属植物は以下に掲げるものを包含する。
【０００７】
Ｂｉｄｅｎｓ ｐｉｌｏｓａ Ｌ．（コセンダングサ、コシロノセンダングサ、咸豊草）
Ｂｉｄｅｎｓ ｐｉｌｏｓａ Ｌ ．ｖａｒ． ｍｉｎｏｒ （Ｂｌｕｍｅ）Ｓｈｅｒｆｆ （シロバナセンダングサ、シロノセンダングサ、コシロノセンダングサ、コセンダングサ、咸豊草）
Ｂｉｄｅｎｓ ｐｉｌｏｓａ Ｌ． ｖａｒ． ｂｉｓｅｔｏｓａ Ｏｈｔａｎｉ ｅｔ Ｓ．Ｓｕｚｕｋｉ（アワユキセンダングサ）
Ｂｉｄｅｎｓ ｐｉｌｏｓａ Ｌ． ｆ． ｄｅｃｕｍｂｅｎｓ Ｓｃｈｅｒｆｆ （ハイアワユキセンダングサ）
Ｂｉｄｅｎｓ ｐｉｌｏｓａ Ｌ． ｖａｒ． ｒａｄｉａｔａ Ｓｃｈｅｒｆｆ （タチアワユキセンダングサ、ハイアワユキセンダングサを含むこともある）
Ｂｉｄｅｎｓ ｐｉｌｏｓａ Ｌ． ｖａｒ． ｒａｄｉａｔａ Ｓｃｈｕｌｔｚ Ｂｉｐｏｎｔｉｎｕｓ （シロノセンダングサ、オオバナノセンダングサ）
Ｂｉｄｅｎｓ ｂｉｔｅｒｎａｔａ Ｌｏｕｒ． Ｍｅｒｒｉｌｌ ｅｔ Ｓｈｅｒｆｆ（センダングサ）
Ｂｉｄｅｎｓ ｂｉｐｉｎｎａｔａ Ｌ．（コバノセンダングサ、センダングサ）
Ｂｉｄｅｎｓ ｃｅｒｎｕａ Ｌ．（ヤナギタウコギ）
Ｂｉｄｅｎｓ ｆｒｏｎｄｏｓａ Ｌ．（アメリカセンダングサ、セイタカタウコギ）
Ｂｉｄｅｎｓ ｐａｒｖｉｆｌｏｒａ Ｗｉｌｌｄ （ホソバノセンダングサ）
Ｂｉｄｅｎｓ ｒａｄｉａｔａ Ｔｈｕｉｌｌ． ｖａｒ． ｐｉｎｎａｔｉｆｉｄａ （Ｔｕｒｃｚ．）Ｋｉｔａｍｕｒａ（エゾノタウコギ）
Ｂｉｄｅｎｓ ｔｒｉｐａｒｔｉｔａ Ｌ．（タウコギ）
【０００８】
上記センダングサ属植物の中で、特にビデンス・ピローサ（Ｂｉｄｅｎｓ ｐｉｌｏｓａ）類が好ましい。
上記センダングサ属植物は通常、生の植物を天日乾燥または熱風（例えば７０〜８０℃）乾燥したもの、又は蒸気で、例えば１時間〜１時間半程度蒸した後、乾燥したものを使用してもよい。
上記センダングサ属植物の使用部位は、根、地上部（茎、葉、花等）又は全草何れの部位を用いてもよい。特に、葉及び茎の部分を使用することが効力の点において好ましい。
【０００９】
ショウガ科植物としては、各種のショウガ、ウコン等が挙げられる。食用、薬用等に用いられるものでもよい。ショウガ科植物は、乾燥または粉砕された市販品を用いてもよく、または栽培した植物を乾燥して粉砕、若しくは蒸煮した後乾燥して粉砕したものを用いてもよい。ショウガ科植物の使用部位は少なくとも根を含む部分である。根部分のみを用いてもよく、または根と茎、葉等その他の部分を用いてもよい。本発明の効果の上で特に好ましいのは根の部分である。
【００１０】
本発明の抽出物製造方法において、上述したセンダングサ属植物、ショウガ科植物の他にさらに風味改善等の目的で他の植物を加えてもよい。他の植物としてはセンダングサ属植物、ショウガ科植物からの成分の抽出を阻害しないもの、また本発明の抽出物の効果を阻害しないものであればよい。具体的には、大麦等が挙げられる。大麦は特に風味の改善の目的で用いることができ、その場合には焙煎した大麦を用いることがさらに好ましい。大麦等を添加する場合には、ショウガ科植物に続いてセンダングサ属植物に添加し、その後抽出を行ってもよい。また、別途水等で抽出したものを本発明の方法により製造されたセンダングサ属植物抽出物に添加してもよい。
センダングサ属植物及びショウガ科植物以外の植物を添加する場合の混合比率は、センダングサ属植物乾燥重量に対して、乾燥重量基準で１０〜４００質量％である。
【００１１】
また、本発明の抽出物製造方法においてさらに酸化防止の目的で他の成分または植物を加えてもよい。他の成分または植物として具体的にはアセロラ抽出物またはアセロラが挙げられる。このような成分または植物を添加する場合には、ショウガ科植物に続いてセンダングサ属植物に添加してもよく、また、抽出後に添加してもよい。このような成分は、センダングサ属植物乾燥重量に対して、乾燥重量基準で１〜５０質量％添加してもよい。
【００１２】
本発明の方法は、センダングサ属植物にショウガ科植物を混合して抽出溶媒により抽出することを特徴とする。
本発明の方法の一つの実施態様を示すと、上述したようにセンダングサ属植物を乾燥したもの又は蒸気で蒸した後乾燥したものに対して、乾燥または蒸気で蒸した後乾燥し、粉砕したショウガ科植物を添加し、常温又は加温下に、溶媒を添加して抽出する。抽出方法としては例えば、浸漬して静置、またはソックスレー抽出器等の抽出器具を用いて抽出物を得ることもできる。
【００１３】
センダングサ属植物に対するショウガ科植物の混合比率は、センダングサ属植物の乾燥全重量に対してショウガ科植物が乾燥重量基準で０．０１〜２０質量％であることが好ましく、より好ましくは０．１〜５質量％である。ショウガ科植物の量が多くなりすぎるとショウガ科植物により皮膚に過剰な刺激を与えたり、ショウガ科植物の独特の風味が強くなりすぎるからであり、また少なすぎると本発明の効果が十分に得られないからである。
【００１４】
抽出に使用される溶媒の例としては、水、メタノール、エタノール、プロパノール、ブタノール、エチレングリコール、プロパンジオール、ブタンジオール、グリセリン等のアルコール類、並びにこれらの含水物、アセトン、エチルメチルケトン、クロロホルム、塩化メチレン及び酢酸エチル、並びにそれらの含水物を用いてもよい。また、上記溶媒を二種以上含む混合物であってもよい。溶媒の添加量は、例えば用いる植物の合計乾燥重量１ｋｇに対して１Ｌ〜１００Ｌ程度使用することができる。
抽出時の温度は、通常、室温〜沸点程度で行うことができる。また、抽出時間は、温度や溶媒にもよるが、室温〜沸点程度で抽出を行う場合には、１〜３００時間程度の範囲にわたって行うことができる。
【００１５】
抽出液は必要により溶媒を留去濃縮して濃縮物または固形物（乾燥物）としてもよい。濃縮物として使用する場合、濃度を調整した後そのまま用いてもよい。また、抽出物は、脱色、不用物除去のため活性炭処理、ＨＰ２０等の樹脂処理、低温放置、瀘過等の処理を施してから用いてもよい。さらに当該抽出物を適当な分離手段、例えばゲル瀘過法やシリカゲルカラムクロマト法、又は逆相若しくは順相の高速液体クロマト法により活性の高い画分を分画して用いることもできる。
【００１６】
抽出物はそのまま、あるいは濃縮物または固形物（例えば粉末）として、内服または外用として用いてもよい。また、抽出物、抽出物の濃縮物または固形物は、水又は水−低級アルコール等の溶媒に溶解してもよい。また使用目的に応じて他の成分を混合して使用してもよい。
【００１７】
本発明の組成物としては、高血糖治療剤または高血糖予防剤、皮膚炎治療剤または皮膚炎予防剤、花粉症治療剤または花粉症予防剤、化粧品等が挙げられる。
本発明のセンダングサ属植物抽出物を含む組成物は、高血糖改善作用を示し、高血糖治療剤または高血糖予防剤として用いることができる。また、本発明のセンダングサ属植物抽出物を含む組成物は高血糖改善作用を示す機能性食品としても有用である。高血糖治療剤または高血糖予防剤として用いる場合には、組成物の全重量に対して抽出物の乾燥重量換算で通常０．０１〜２０質量％程度の量が配合される。
【００１８】
本発明のセンダングサ属植物抽出物を含む組成物は、皮膚炎の改善作用を示し、皮膚炎治療剤または皮膚炎予防剤として用いることができる。用いることができる皮膚炎としては、アレルギー性皮膚炎、アトピー性皮膚炎、湿疹、接触皮膚炎等が挙げられる。また、本発明のセンダングサ属植物抽出物を含む組成物は皮膚炎改善作用を示す機能性食品としても有用である。皮膚炎治療剤または皮膚炎予防剤として用いる場合には、組成物の全重量に対して抽出物の乾燥重量換算で通常０．０１〜２０質量％程度の量が配合される。
【００１９】
本発明のセンダングサ属植物抽出物を含む組成物は、花粉症の改善作用を示し、花粉症治療剤または花粉症予防剤として用いることができる。用いることができる花粉症としては、杉花粉症、檜花粉症、カモガヤ花粉症等が挙げられる。また、本発明のセンダングサ属植物抽出物を含む組成物は皮膚炎改善作用を示す機能性食品としても有用である。皮膚炎治療剤または皮膚炎予防剤として用いる場合には、組成物の全重量に対して抽出物の乾燥重量換算で通常０．０１〜２０質量％程度の量が配合される。
【００２０】
本発明のセンダングサ属植物抽出物を含む組成物は、化粧品としても用いることができる。すなわち、内服若しくは外用により皮膚の保湿作用に効果があり、美肌剤等として使用することができる。また、日焼け止め作用も顕著であり、日焼け止めクリーム等の化粧品としても使用することができる。また、本発明のセンダングサ属植物抽出物を含む組成物は美肌作用を示す機能性食品としても有用である。化粧品が、美肌剤、または日焼け止め剤である場合、化粧品中に化粧品の全重量に対して抽出物の乾燥重量換算で通常は１０質量％程度以下、好ましくは１〜５質量％の量が配合される。化粧品として用いる場合には、化粧水、乳液、クリーム、パック等いずれの形態で用いても構わない。
【００２１】
以下試験例および実施例により本発明を具体的に説明するが、本発明はこれらに限定されるものではない。
【実施例】
（実施例１）内服による高血糖改善作用
ビデンス・ピローサ（タチアワユキセンダングサ）を流水で洗浄して異物を取り除いた後、数センチ程度に裁断した。裁断物を底が網状の円筒形容器に入れ、１時間〜１時間半程度蒸気で蒸煮した。その後ニーダーで圧潰し、７０〜８０℃程度の熱風で乾燥した（参照、特開平２００１−１７８３９０号公報）。
得られた乾燥物を、Ａ）ビデンス・ピローサ単独、Ｂ）ビデンス・ピローサとショウガとの混合物、およびＣ）ビデンス・ピローサとウコンとの混合物とし、さらにそれぞれを焙煎した大麦で倍散して、各３ｇをティーバッグに仕立てた。このティーバッグ１個に約１．２ Ｌの水を加えて５分間煮沸抽出し、その全量を１日で飲んでもらい、これを３カ月間毎日行った。調製したティーバッグの種類と内容は表１の通りである。
【００２２】
【表１】ティーバッグの組成（質量％）
【００２３】
【表２】高血糖者の空腹時血糖値（ｍｇ／ｄＬ）の変化
【００２４】
上記表からわかるようにビデンス・ピローサ単独（Ａ）でも若干の血糖降下作用が認められるが、ショウガとの混合物の抽出物（例えばＣ、Ｄ、Ｅ）では個人差はあるもののいずれも血糖降下作用がより顕著に認められた。またウコンとの混合物の抽出物（例えば、Ｇ、Ｈ）についても血糖降下作用が認められた。
またビデンス・ピローサ単独では、独特の野草味、野草臭があって甚だ飲みにくいものであるが、これに少量のショウガ或いはウコンを配合するとこの臭味は著明に改善され格段に飲みやすいものになった。また、本実験で血糖降下作用の他の評価要因として、香味に関して言えば好みに個人差はあるもののショウガまたはウコンの配合量が１〜５％、特に１〜３％が飲みやすく、７％では些か飲みにくいことが分かった。
【００２５】
（実施例２）内服による小児皮膚炎の改善作用
次の４例は、医師の診察を受けアトピー性皮膚炎と診断されたが、医師からの処方による薬剤を使用せず、ビデンス・ピローサ抽出物を使用した例である。最初１カ月は実施例例１の組成Ａ（ビデンス・ピローサ単独）を飲ませたところ、改善され０る傾向が見られたものの一進一退であった。２カ月目から組成Ｃ（ビデンス・ピローサ＋ショウガ）またはＧ（ビデンス・ピローサ＋ウコン）に変更したところ、下記に示すようにいずれも著名な改善が見られた。この間に排便の回数も増え便の状態も改善された。
【００２６】
内服による小児皮膚炎の改善（組成ＣまたはＧに変更後）
Ｋ子（３カ月女） 組成Ｃ使用
発症部位：顔・耳・頸の周囲、四肢の付根や関節部。
投与法：入浴前後毎回５０〜６０ｍＬ、授乳前毎回３０〜４０ｍＬ。
１日量：約１０回、約４００ｍＬ。
経過：１カ月で顔・耳、２カ月で頸の症状消退、３〜４カ月でほぼ完治
【００２７】
Ｎ子（４カ月女） 組成Ｇ使用
発症部位：耳・頸・頬・腕の関節部。
投与法：就眠前・授乳前毎回３０〜６０ｍＬ。
１日量：約１０回、約５００ｍＬ。
経過：約２週間でまず頬が、約５週間で他の部位の症状もほとんど消失した。
【００２８】
Ｓ子（１歳半女） 組成Ｃ使用
発症部位：手・腕・顔・ふくらはぎ。
投与法：随時。
１日量：１０回以上、１〜１．２ Ｌ。
経過：約１．５カ月で顔の発疹が、他は１〜２カ月遅れ消失、その後半年再発なし。
【００２９】
Ｔ夫（２歳半男） 組成Ｃ使用
発症部位：耳・頸・腕・膝の裏。
投与法：随時。
１日量：１０回以上、１．５ 〜１．８Ｌ。
経過：約２カ月で発疹も痒みも次第に消退し３カ月で完治、その後半年再発なし。
【００３０】
結果を総合するとショウガやウコンを混合していない組成Ａを使用した期間は一進一退であったが、本発明の組成ＣまたはＧに変更してからは症状が顕著に改善された。
【００３１】
（実施例３）内服によるアトピー性皮膚炎の改善
４２歳女性、２０歳の時から顔面〜頸・胸にかけても重症のアトピー性皮膚炎が出て長期間ステロイド剤を使用していた。平成１４年１１月からステロイド剤を止めて、試験例１の組成Ａ（ビデンス・ピローサ単独）を１日２〜２．５Ｌ飲んだところ１カ月頃から少し改善が見られ始めたが４カ月に入った頃にやや逆戻りしたので、本発明の組成Ｂ（ビデンス・ピローサ＋ショウガ）に変更したところ、通算約７カ月で皮膚の色素斑もほとんど消え、一見して分からないくらいに改善が認められた。
【００３２】
（実施例４）内服による主婦湿疹の改善
４８歳女性、約２年前に洗剤で手が荒れた時から、四肢の主として外側に広範囲に、皮膚の隆起と硬化を伴い色素斑を残す皮膚炎を発症、病院でステロイド外用剤の投与を受け却って増悪した。平成１３年２月からステロイド剤を止めて試験例１の組成Ａ（ビデンス・ピローサ単独）のティーバッグを１日約３Ｌ（２包分）を飲んだところ、約２カ月で炎症はやや治まる傾向が見えたが別の箇所に新たな発症が見られたので、本発明の組成Ｄ（ビデンス・ピローサ＋ショウガ）に変更したところ次第に改善されて色素斑や皮膚の隆起も退縮し、通算約５カ月でほぼ全快に近い状態となった。
【００３３】
（実施例５）内服による夏季潰瘍の改善
皮膚の毛細血管の血行が障害されて膝から下の足、特に関節付近の皮膚に疼痛を伴う潰瘍を生じ、春から夏になると再発しやすい夏季潰瘍と呼ばれる特に女性に多く報告されている難病がある。そのような患者７人に実施例１のＣ（ビデンス・ピローサ＋ショウガ）を試みに飲んでもらった（早い人は平成１２年５月下旬から、遅い人は７月下旬から、１日１包、１〜１．２Ｌ）ところ、その夏は７人全員に潰瘍の再発が見られなかった。この時点で再発率ゼロ、そして飲まなかった９人中５人、関連病院の１３例中９人が再発した。そこで翌１３年３月から本発明以前の単純な組成のＡ（ビデンス・ピローサ単独）に変更したところ３人に潰瘍が再発し、内１人は軽症であったが２人はかなり重症で、再び本発明の組成Ｃに戻して軽症の１人は軽快したが重症の２人は改善せず、翌１４年６月になって漸く潰瘍の新生は見られなくなった。症例を逐次追加し１４年の夏は最初の７人を含めて１５人中１３人が再発しなかった。組成Ａを使用した時期の潰瘍の数、大きさ、程度などを含めた観察結果から組成Ｃは明らかに組成Ａよりも有効性が優れていた。
【００３４】
（実施例６）ビデンス・ピローサ抽出条件の違いによる抗炎症作用比較試験
実施例１〜５において、内服剤としてビデンス・ピローサ単味よりも少量のショウガやウコンを配合して煎じたものの方がより有効であることが見出されたが、この理由として、ａ）ショウガやウコンの作用が、ビデンス・ピローサの作用に単に付加されたか、ｂ）ショウガやウコンの添加によってビデンス・ピローサの作用が相乗的に増強されたことが考えられる。一方、実施例１に示したような血糖降下作用はショウガやウコンには報告されておらず、ａ）の解釈は成立しにくい。これを確認すため抗炎症作用を代表として選び、ヒトでのパッチテストを行った。
【００３５】
［抽出条件］
ビデンス・ピローサ水：実施例１と同じ材料１００ｇに精製水２Ｌを加え、９０〜１００℃で５時間抽出した後、濾紙を用いて濾過し、瀘液を濃縮して抽出物全量を１００ｍＬとした。
ショウガ水：乾燥ショウガ粉末１００ｇをビデンス・ピローサと同様に処理して抽出物全量を１００ｍＬとした。
ウコン水：乾燥ウコン粉末も同様に処理した。
混合抽出物１：ビデンス・ピローサ９４ｇ＋ショウガ６ｇを同様に処理した。
混合抽出物２：ビデンス・ピローサ９４ｇ＋ウコン６ｇを同様に処理した。
【００３６】
［パッチテスト試料］
試料１：ビデンス・ピローサ水単独
試料２：ビデンス・ピローサ水９４部＋ショウガ水６部の混液
試料３：ビデンス・ピローサ水９４部＋ウコン水６部の混液
試料４：混合抽出物１
試料５：混合抽出物２
【００３７】
［消炎効果の比較試験］
１）接触皮膚炎治療効果
起炎剤（ラウリル硫酸ナトリウムの１０％水溶液）を上腕内側に４時間クローズドパッチし、９０分後、試料（０．５ｍＬ）を１６時間クローズドパッチして１２０時間まで観察した結果は表３に示すとおりである。
【００３８】
【表３】消炎効果の比較試験
【００３９】
２）接触皮膚炎予防効果
起炎剤を試料に混合して上腕内側に５時間クローズドパッチし、７２時間まで観察した。その結果は表４に示すとおりである。
【００４０】
【表４】炎症抑制効果の比較試験
【００４１】
（実施例７）美肌用スキンクリームの効果
実施例６で調製した試料を用いて表５に示す組成のスキンクリームを調製し、その効果及び使用感について、下記の方法に従い評価を行った。
［製造方法］
表５のＡグループ成分を加温溶解し８０℃に保った油相に、Ｂグループ成分を同様に処理した水相を加えて乳化し、冷却して製品（スキンクリーム１〜３）とした。
［評価方法］
上述したように製造した製品を、製品１ｇ／日の使用量で、各クリームについて２０名づつの被験者の顔と手に塗布して、１ヶ月間使用することにより評価した。
【００４２】
【表５】美肌用スキンクリームの比較評価
＊２：スキンクリーム２（本発明）
＊３：スキンクリーム３（本発明）
【００４４】
表５のとおり、対照である従来法のビデンス・ピローサ水を混合したスキンクリーム１に比べて本発明の混合抽出物１及び２を添加したスキンクリーム２及び３の方が顕著な美肌効果及び使用感の改善が認められた。またカブレを訴えた者は全くいなかった。
【００４５】
（実施例８）乳液によるアトピー性皮膚炎の軽減
実施例１と同じビデンス・ピローサ９４ｇにショウガ６ｇを混合し、エタノール２Ｌを加え、常温で７日間抽出した後、瀘液を濃縮して抽出物全量を１００ｍＬとして、混合抽出物３を製造した。また別にエタノールの代りに１，３−ブチレングリコールを用いて、混合抽出物４を製造した。対照としてビデンス・ピローサ１００ｇをエタノールまたは１，３−ブチレングリコールで同様に処理したもの（ビデンス・ピローサ＋エタノール、及びビデンス・ピローサ＋１，３−ブチレングリコール）を用意した。
［乳液の製造方法］
表６のＡグループ成分を加温溶解し８０℃に保った油相に、Ｂグループ成分を同様に処理した水相を加えて乳化し、冷却して製品（乳液１〜４）とした。
【００４６】
［評価方法］
これらの抽出物を配合した表６に示す組成の乳液を用い、アトピー性皮膚炎患者（全１０名）で評価を行った。アトピー性皮膚炎が発症した部位に、０．５ｇ／ｃｍ２程度の使用量で、１ヶ月間使用した。その効果及び使用感につき下記の方法に従い評価を行った。
【表６】乳液によるアトピー性皮膚炎の改善
表６の結果に見られるように、エタノールで抽出した場合も１，３−ブチレングリコールで抽出した場合も本発明の実施例（混合抽出物３及び４）の方が比較（ビデンス・ピローサ＋エタノール、ビデンス・ピローサ＋１，３−ブチレングリコール）に比べて、顕著な消炎効果と、カユミ止め効果の改善が認められた。
【００４９】
（実施例９）日焼け止めクリームの効果の改善
［日焼け止めクリームの製造方法］
表７の成分Ａグループを加温溶解し８０℃に保った油相に、成分Ｂグループを同様に処理した水相を加えて乳化し、冷却して製品（日焼け止めクリーム１〜４）とした。なお、表７における混合抽出物３、混合抽出物４、ビデンス・ピローサ＋エタノール、及びビデンス・ピローサ＋１，３−ブチレングリコールは、実施例８において調整したものと同じである。
［評価方法］
得られて製品を人の背部に塗布しその上に紫外線ＵＶＢを照射し、日本化粧品工業会の測定法に準じ日焼け防止効果ＳＰＦを算出して評価を行った。
【００５０】
【表７】日焼け止めクリームの効果の改善
＊２：日焼け止めクリーム２（本発明）
＊３：日焼け止めクリーム３（比較例）
＊３：日焼け止めクリーム４（本発明）
【００５１】
表７の結果に見られるように、エタノールで抽出した場合も１，３−ブチレングリコールで抽出した場合も本発明の実施例（混合抽出物３及び４）の方が比較（ビデンス・ピローサ＋エタノール、ビデンス・ピローサ＋１，３−ブチレングリコール）に比べて、顕著な日焼け防止効果の改善が見られた。
【００５２】
（実施例１０）
タチアワユキセンダングサの地上部を刈り取り洗浄後、適当な長さに刻んで約１時間蒸煮し、８０℃の熱風を送って通風乾燥した。その１０ｋｇに、乾燥したショウガ３ｋｇを添加して、水／メタノール／１，３−ブチレングリコール（１／１／２、ｖ／ｖ）混液２００Ｌを加えて室温で１週間放置して抽出し、濾過を行い、濾紙上の不溶物を十分に絞って濾液を得た。得られた濾液に乳糖０．５ｋｇを加えて噴霧乾燥し、２．９ｋｇの抽出物粉末を得た。
この抽出物粉末に賦形剤としてアビセル（結晶セルロース）０．３ｋｇと滑沢剤として蔗糖脂肪酸エステル０．１ｋｇを加え、打錠して２５０ｍｇの錠子とした。
【００５３】
杉花粉症に対する効果
くしゃみ、鼻水、目のかゆみ等の症状を有する杉花粉症患者５人を被験者とした。５人とも、医師の指導のもと、抗アレルギー剤、マスクの着用等の措置を講じていたが依然として症状が緩和されなかった。この５人の被験者に、医者の薬を止めて本発明の錠子を空腹時に３錠ずつ１日３回飲んでもらったところ、いずれも以下に示すように１０日以内にはすっきりと症状がなくなった。その後服用を継続している限り症状が出なかったが、服用を二三日忘れると発症した。
【００５４】
１）Ｙ．Ｓ．男性４７歳（会社管理職）５〜７日でほとんど症状がなくなり、その後再発症しなかった。
２）Ｓ．Ｔ．男性３９歳（会社営業職）約１週間で症状が非常に軽くなった。
３）Ｋ．Ｉ．男性４４歳（地方公務員）４〜５日でほとんど症状がなくなり、その後再発症しなかった。
４）Ｍ．Ｉ．男性７５歳（会社役員）数日後からほとんど花粉症の症状が無くなった。
５）Ｒ．Ｉ．女性３９歳（会社事務職）１０日ほど経ってから極めて症状が軽くなった。
【発明の効果】
以上の通り、本発明の方法により得られた抽出物を含む組成物は、高血糖、皮膚炎、花粉症等の治療及び予防剤として、また美肌剤や日焼け止め等の化粧品として優れた効果を示し、さらにビデンス・ピローサ単独の抽出物より明らかに優れた効果を示す。[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to a composition comprising an extract of the genus Sendangsa, in particular, an extract of Bidens pirosa, and to a method for producing the extract.
[0002]
[Prior art]
Bidens Pirosa is a herb that has been around for a long time, and is used as a folk herb for decoction of dried ground parts, and as antipyretic, detoxifying, antiphlogistic, analgesic, antidiarrheal, diuretic, hepatitis, nephritis, cecumitis, diabetes, cystitis, urethritis.・ It has been used for rheumatoid arthritis, bronchitis, swelling, gastrointestinal diseases, diarrhea, indigestion, etc., and has been used externally for swelling and pain in the throat and bruises. In particular, for severe cecal inflammation, if you drink decoction, eat fresh shoots, or grind about 60 g and drink the juice (you can add a little nectar or salt), inflammation and pain will stop even in severe cases with adhesions It has been. It is also suggested that swollen sore throat can be used as a mouthwash at the same time as drinking it. In addition, it has some bactericidal and bacteriostatic properties, and has been used as an external medicine, such as bruising and swelling, washing or applying topically.
Conventionally, as a method of using Bidence pilosa, a substance extracted with water (decoction) for internal use or external use has been used. For example, there is a method in which 10 to 15 g of a raw plant is decocted with 400 mL of water, boiled down to half the amount, and then drunk. In addition, many people use Bidens Pirosa as a household folk medicine in Taiwan by picking wild ones such as Dokudami and Gennoshoko in Japan. At the pharmacies used by Chinese medicine (Chinese medicine), Xianfeng soybean extract powder can be obtained. Some flowers are yellow, but white is said to be good for medicinal purposes.
For the purpose of researching the components of Bidens pirosa, water extraction or extraction with a solvent such as methanol or ethanol is performed (for example, see Non-Patent Document 1). However, extraction is performed by adding other additives to Bidens pirosa. No examples are known.
[0003]
[Non-patent document 1]
Paul V. Tan et al. , Journal of Ethnopharmacology 73, 415 (2000).
[0004]
[Problems to be solved by the invention]
It is an object of the present invention to provide a method for producing an extract of a plant of the genus Sendanga, which further enhances the usefulness and effectiveness of the plant of the genus Sendangsa, and a composition containing the extract.
[0005]
[Means for Solving the Problems]
The present inventor has conducted various studies on the plant of the genus Sendangsa, and as a result, a composition containing an extract obtained by previously mixing and extracting a ginger plant such as ginger or turmeric with the plant of the genus Sendanga, the composition extracted with the plant of the genus Sendanga alone It has been found to be more effective in the treatment and prevention of hyperglycemia, dermatitis, hay fever and the like, and also as cosmetics such as skin beautifiers and sunscreens. In addition, it has been found that an extract obtained by extracting a mixture of a plant belonging to the genus Sendangsa and a ginger plant has a superior effect, that is, a synergistic effect, compared with a mixture obtained by extracting a mixture of the plants alone.
That is, the present invention provides a composition containing a Sendangusa plant extract produced by mixing a Zingiberaceae plant with a Sendangusa plant and extracting the mixture with an extraction solvent.
The present invention also provides a method for producing an extract of a genus Sendangsa, comprising mixing a plant of the genus Genus with a plant of the genus Genus and extracting with an extraction solvent.
[0006]
BEST MODE FOR CARRYING OUT THE INVENTION
As described in JP-A-2001-178391 and JP-A-2001-233427, the plants of the genus Sendangsa used in the present invention are a group of plants referred to in the scientific name as the genus Bidens. Although the variety crosses a wide variety with each other, there are many varieties, and confusion is seen in botany, and the correspondence of scientific names, Japanese names, Chinese names is also mixed, and it is extremely difficult to identify, but in the present invention, The Sendangusa plants used include the following.
[0007]
Bidens pilosa L. (Coleoptera, Rhododendron L., Xianfeng)
Bidens pilosa L. var. minor (Blume) Sheff (Shirobana Sengusa, Shirono Sengusa, Koshirono Sengusa, Sengokusa, Hampungsa)
Bidens pilosa L. var. bisetosa Ohtani et S .; Suzuki
Bidens pilosa L. f. decumbens Schaffff
Bidens pilosa L. var. radiata Scherff (may also include Tachiawayukisendansa and Hiawayukisendangusa)
Bidens pilosa L. var. radiata Schultz Bicontinus
Bidens bitternata Lour. Merrill et Sherff
Bidens bipinnata L. (Kobano Sendangsa, Sendangsa)
Bidens cernua L. et al. (Salix)
Bidens frontosa L.A. (American Sendangusa, Seita Katawagi)
Bidens parviflora Willd
Bidens radiata Twill. var. pinnatifida (Turcz.) Kitamura (Ezonotaucogi)
Bidens tripartita L. (Taukogi)
[0008]
Among the above-mentioned plants of the genus Sendangsa, Bidens pilosa are particularly preferred.
The above-mentioned plants of the genus Sendanga are usually obtained by drying a raw plant by sun drying or hot air (eg, 70 to 80 ° C.), or by steaming with steam, for example, for about 1 hour to 1.5 hours, and then drying. Is also good.
The site of use of the above-mentioned plants of the genus Sendangsa may be any of the root, the above-ground part (stem, leaf, flower, etc.) or the whole plant. In particular, the use of leaf and stem portions is preferred in terms of efficacy.
[0009]
Examples of the ginger plant include various ginger and turmeric. It may be used for food, medicine, and the like. As the ginger plant, a dried or pulverized commercial product may be used, or a cultivated plant may be dried and pulverized, or steamed and then dried and pulverized. The site where the ginger plant is used is a portion including at least the root. Only the root part may be used, or other parts such as root and stem, leaf, etc. may be used. Particularly preferred in view of the effect of the present invention is a root portion.
[0010]
In the method for producing an extract of the present invention, other plants may be added for the purpose of improving flavor and the like in addition to the above-mentioned plants of the genus Sendangsa and the plants of the family Zingiberaceae. Other plants may be those that do not inhibit the extraction of components from the plants of the genus Sendangsa and the plants of the family Zingiberaceae, and those that do not inhibit the effect of the extract of the present invention. Specific examples include barley. Barley can be used particularly for the purpose of improving flavor, and in that case, roasted barley is more preferably used. When barley or the like is added, it may be added to a plant belonging to the genus Sendanga following the ginger plant, and then extraction may be performed. Alternatively, the extract extracted with water or the like may be added to the extract of the genus Sendanga produced by the method of the present invention.
The mixing ratio when a plant other than the plant of the genus Sendangsa and the plant of the family Zingiberaceae is added is 10 to 400% by mass based on the dry weight of the plant of the genus Sendangsa based on the dry weight.
[0011]
In the method for producing an extract of the present invention, other components or plants may be further added for the purpose of preventing oxidation. Specific examples of other components or plants include acerola extract or acerola. When such a component or a plant is added, it may be added to a plant of the genus Sengusa following the ginger plant, or may be added after extraction. Such a component may be added in an amount of 1 to 50% by mass based on the dry weight of the plant of the genus Sendangsa.
[0012]
The method of the present invention is characterized in that a ginger plant is mixed with a plant of the genus Sendangsa and extracted with an extraction solvent.
According to one embodiment of the method of the present invention, dried or steamed steamed and dried and pulverized ginger of the genus Sendangsa is dried or steamed and dried as described above. A family plant is added, and a solvent is added at room temperature or under heating to perform extraction. As an extraction method, for example, the extract can be obtained by immersing the sample and leaving it still, or using an extraction device such as a Soxhlet extractor.
[0013]
The mixing ratio of the Zingiberaceae plant to the Sendangusa genus plant is preferably 0.01 to 20% by mass, more preferably 0.1 to 20% by mass, based on the dry weight of the Zingiberaceae plant based on the total dry weight of the Sendangusa genus plant. 5% by mass. If the amount of the ginger plant is too large, the skin is excessively stimulated by the ginger plant, or the unique flavor of the ginger plant becomes too strong.If the amount is too small, the effect of the present invention is sufficiently obtained. It is not possible.
[0014]
Examples of solvents used for extraction include water, methanol, ethanol, propanol, butanol, ethylene glycol, propanediol, butanediol, alcohols such as glycerin, and hydrates thereof, acetone, ethyl methyl ketone, chloroform, Methylene chloride and ethyl acetate, and their hydrates may be used. Further, a mixture containing two or more of the above solvents may be used. The amount of the solvent to be added can be, for example, about 1 L to 100 L based on 1 kg of the total dry weight of the plant to be used.
The temperature at the time of extraction can be usually from room temperature to about the boiling point. The extraction time depends on the temperature and the solvent, but when the extraction is performed at room temperature to about the boiling point, the extraction can be performed over a range of about 1 to 300 hours.
[0015]
If necessary, the extract may be concentrated by distilling off the solvent to obtain a concentrate or a solid (dry matter). When used as a concentrate, it may be used as it is after adjusting the concentration. The extract may be used after being subjected to a treatment such as an activated carbon treatment, a resin treatment such as HP20, a standing at a low temperature, and a filtration in order to remove color and remove unnecessary substances. Further, the extract can be used by fractionating a highly active fraction by a suitable separation means, for example, gel filtration, silica gel column chromatography, or reversed-phase or normal-phase high-performance liquid chromatography.
[0016]
The extract may be used as such, or as a concentrate or solid (eg, a powder), for internal use or for external use. Further, the extract, the concentrate of the extract, or the solid substance may be dissolved in water or a solvent such as water-lower alcohol. Further, other components may be mixed and used according to the purpose of use.
[0017]
Examples of the composition of the present invention include an agent for treating or preventing hyperglycemia, an agent for treating dermatitis or an agent for preventing dermatitis, an agent for treating or preventing hay fever, and cosmetics.
The composition containing the extract of the genus Sendangsa of the present invention exhibits a hyperglycemic ameliorating effect and can be used as a therapeutic agent for hyperglycemia or a preventive agent for hyperglycemia. In addition, the composition containing the extract of the genus Sendangusa of the present invention is also useful as a functional food exhibiting a hyperglycemic ameliorating effect. When used as a therapeutic agent for hyperglycemia or a prophylactic agent for hyperglycemia, an amount of usually about 0.01 to 20% by mass in terms of dry weight of the extract is blended with respect to the total weight of the composition.
[0018]
The composition containing the extract of the genus Sendangsa of the present invention exhibits an effect of improving dermatitis and can be used as a therapeutic agent for dermatitis or a preventive agent for dermatitis. Dermatitis that can be used includes allergic dermatitis, atopic dermatitis, eczema, contact dermatitis and the like. Further, the composition containing the extract of the genus Sendangsa of the present invention is also useful as a functional food having a dermatitis ameliorating effect. When used as a therapeutic agent for dermatitis or a preventive agent for dermatitis, the amount is usually about 0.01 to 20% by mass in terms of the dry weight of the extract based on the total weight of the composition.
[0019]
The composition containing the extract of the genus Sendangusa of the present invention exhibits an effect of improving hay fever, and can be used as a therapeutic agent for hay fever or a prophylactic agent for hay fever. Examples of hay fever that can be used include cedar hay fever, cypress hay fever, and duck hay fever. Further, the composition containing the extract of the genus Sendangsa of the present invention is also useful as a functional food having a dermatitis ameliorating effect. When used as a therapeutic agent for dermatitis or a preventive agent for dermatitis, the amount is usually about 0.01 to 20% by mass in terms of the dry weight of the extract based on the total weight of the composition.
[0020]
The composition containing the extract of the genus Sendanga of the present invention can also be used as cosmetics. In other words, when taken internally or externally, it has an effect on the moisturizing effect of the skin, and can be used as a beautifying agent or the like. In addition, it has a remarkable sunscreen effect and can be used as cosmetics such as sunscreen creams. In addition, the composition containing the extract of the genus Sendangsa of the present invention is also useful as a functional food exhibiting a beautiful skin effect. When the cosmetic is a skin beautifying agent or a sunscreen, the amount of the extract is usually about 10% by mass or less, preferably 1 to 5% by mass in terms of the dry weight of the extract, based on the total weight of the cosmetic. Is done. When used as cosmetics, it may be used in any form such as lotion, milky lotion, cream, pack and the like.
[0021]
Hereinafter, the present invention will be described specifically with reference to Test Examples and Examples, but the present invention is not limited thereto.
【Example】
(Example 1) Hyperglycemia improving effect by oral administration
Bidens Pirosa was washed with running water to remove foreign substances, and then cut into several centimeters. The cut material was placed in a cylindrical container having a net-like bottom, and steamed for about one hour to one and a half hours with steam. Thereafter, it was crushed with a kneader and dried with hot air of about 70 to 80 ° C. (see JP-A-2001-178390).
The resulting dried product is made into A) Bidens-Pirosa alone, B) A mixture of Bidens-Pirosa and ginger, and C) A mixture of Bidens-Pirosa and turmeric, each of which is triturated with roasted barley. And 3 g each were made into a tea bag. Approximately 1.2 L of water was added to one of the tea bags, and the mixture was boiled and extracted for 5 minutes, and the whole amount was drunk in one day, and this was performed every day for three months. Table 1 shows the types and contents of the prepared tea bags.
[0022]
[Table 1] Composition of tea bags (% by mass)
[0023]
Table 2 Changes in fasting blood glucose (mg / dL) of hyperglycemic subjects
[0024]
As can be seen from the above table, a slight hypoglycemic effect was observed even with Bidens-Pirosa alone (A), but an extract of a mixture with ginger (for example, C, D, E) showed a hypoglycemic effect although there were individual differences. Was more remarkably recognized. Extracts of a mixture with turmeric (eg, G, H) also exhibited a hypoglycemic effect.
In addition, Bidence Pirosa alone has a unique wild grass taste and wild grass smell, which is extremely difficult to drink. However, when a small amount of ginger or turmeric is added, this smell is markedly improved and it becomes much easier to drink. became. As another evaluation factor of the hypoglycemic effect in this experiment, although there is an individual difference in taste when it comes to flavor, 1 to 5%, especially 1 to 3%, of ginger or turmeric is easy to drink. It turned out to be a little difficult to drink.
[0025]
(Example 2) Improvement of childhood dermatitis by oral administration
The following four cases were diagnosed with atopic dermatitis upon consultation with a physician, but did not use a drug prescribed by the physician and used an extract of Bidens pirosa. In the first month, when the composition A of Example 1 (Bidens-Pirosa alone) was given, it was improved, but the tendency toward zero was observed. When the composition was changed from the second month to the composition C (Bidens-Pirosa + Ginger) or G (Bidence-Pirosa + Turmeric), remarkable improvements were observed as shown below. During this time, the number of bowel movements increased and the condition of the bowel movements improved.
[0026]
Improvement of pediatric dermatitis by oral administration (after changing to composition C or G)
K child (3 months old woman) Composition C used
Onset: Around the face, ears and neck, bases and joints of limbs.
Administration method: 50-60 mL each time before and after bathing, 30-40 mL each time before lactation.
Daily dose: about 10 times, about 400 mL.
Progress: face / ear in 1 month, neck disappearance in 2 months, almost complete recovery in 3-4 months
[0027]
N child (four months old) Composition G used
Onset: Ear, neck, cheek, arm joints.
Administration method: 30-60 mL before sleep and before lactation.
Daily dose: about 10 times, about 500 mL.
Progress: The cheeks first disappeared in about 2 weeks, and the symptoms in other parts almost disappeared in about 5 weeks.
[0028]
S child (1 and a half years old) Composition C used
Onset: Hand, arm, face, calf.
Administration method: any time.
Daily dose: 10 times or more, 1 to 1.2 L.
Course: Facial rash disappeared in about 1.5 months, others disappeared 1-2 months later, no recurrence in the latter half year.
[0029]
T husband (2 years and a half male) Composition C used
Onset: Ear, neck, arm, back of knee.
Administration method: any time.
Daily dose: 10 or more, 1.5 to 1.8 L.
Progress: Rash and itch gradually disappeared in about 2 months, and healed completely in 3 months, with no recurrence in the latter half of the year.
[0030]
When the results were combined, the period using composition A containing no ginger or turmeric ebb and flow, but the symptoms were remarkably improved after changing to composition C or G of the present invention.
[0031]
(Example 3) Improvement of atopic dermatitis by oral administration
A 42-year-old woman had severe atopic dermatitis in her face, neck and chest from the age of 20 and had been using steroids for a long time. He stopped using steroids in November 2002 and drank 2 to 2.5 L of composition A of Test Example 1 (Bidence Pirosa alone) a day. When it entered, it reverted a little, so when it was changed to the composition B of the present invention (Bidence Pirosa + Ginger), pigmentation spots on the skin almost disappeared in about 7 months in total, and improvement was recognized at first glance Was.
[0032]
(Example 4) Improvement of housewife eczema by oral administration
A 48-year-old woman develops dermatitis that leaves pigment spots with bulging and hardening of the skin, mainly on the outside of her limbs, since her hands were roughened with detergent about 2 years ago. It got worse. When the steroid was stopped in February 2001 and the tea bag of Composition A (Bidence Pirosa alone) of Test Example 1 was drunk about 3 L / day (2 servings), the inflammation tended to subside in about 2 months. Was observed, but a new onset was observed in another place. Therefore, when the composition D of the present invention was changed to Bidence-Pirosa + Ginger, the composition gradually improved, and pigment spots and skin bulges also regressed. In almost a month, the situation was almost full.
[0033]
(Example 5) Improvement of summer ulcer by oral administration
An intractable disease that is often reported especially in women, called summer ulcers, which cause painful ulcers in the skin below the knees, especially the skin near the joints due to impaired blood circulation of the skin's capillaries, and which tend to recur from spring to summer There is. Seven such patients tried to drink C (vidence pilosa + ginger) in Example 1 (early one from late May 2000, late one from late July 1 capsule daily) However, in the summer, no recurrence of the ulcer was observed in all seven patients. At this point, there was no recurrence rate, and 5 out of 9 people who did not drink and 9 out of 13 cases at the relevant hospital relapsed. Therefore, in March 2013, when the composition was changed to A (Bidence Pirosa alone) having a simple composition before the present invention, ulcers recurred in three of them, one of which was mild but two were quite severe, By returning to the composition C of the present invention again, one mild case was relieved, but two severe cases did not improve, and as of June 2014, ulcer formation did not finally appear. In the summer of 2014, 13 out of 15 cases, including the first 7, did not recur. From the observation results including the number, size, degree, etc. of the ulcers at the time when the composition A was used, the composition C was clearly more effective than the composition A.
[0034]
(Example 6) Comparative test of anti-inflammatory effect by difference of extraction conditions of Bidens-Pirosa
In Examples 1 to 5, it was found that a mixture of a small amount of ginger and turmeric as an oral preparation and decoctioned with a smaller amount of ginger or turmeric was more effective, because, a) ginger It is conceivable that the action of turmeric or turmeric was simply added to the action of Bidens-Pyrosa, or b) the action of Bidens-Pirosa was synergistically enhanced by the addition of ginger or turmeric. On the other hand, the hypoglycemic effect as shown in Example 1 has not been reported for ginger or turmeric, and the interpretation of a) is difficult to be established. To confirm this, the anti-inflammatory effect was selected as a representative and a patch test was performed on humans.
[0035]
[Extraction condition]
Bidens-Pirosa water: 2 L of purified water was added to 100 g of the same material as in Example 1, extracted at 90 to 100 ° C. for 5 hours, filtered using filter paper, and the filtrate was concentrated to a total extract volume of 100 mL. .
Ginger water: 100 g of dried ginger powder was treated in the same manner as in Bidens-Pirosa to make the total amount of extract 100 mL.
Turmeric water: Dried turmeric powder was treated similarly.
Mixed extract 1: 94 g of Bidens-Pirosa + 6 g of ginger were treated similarly.
Mixed extract 2: 94 g of Bidens-Pirosa + 6 g of turmeric were treated similarly.
[0036]
[Patch test sample]
Sample 1: Bidence Pirosa water alone
Sample 2: A mixture of 94 parts of Bidence-Pirosa water and 6 parts of ginger water
Sample 3: A mixture of 94 parts of Bidence-Pirosa water and 6 parts of turmeric water
Sample 4: Mixed extract 1
Sample 5: Mixed extract 2
[0037]
[Comparative test of anti-inflammatory effect]
1) Contact dermatitis treatment effect
The inflammatory agent (10% aqueous solution of sodium lauryl sulfate) was closed-patched on the inner side of the upper arm for 4 hours, and after 90 minutes, the sample (0.5 mL) was closed-patched for 16 hours and observed up to 120 hours. It is as follows.
[0038]
[Table 3] Comparative test of anti-inflammatory effect
[0039]
2) Contact dermatitis prevention effect
The proinflammatory agent was mixed with the sample, and the patch was closed on the inner side of the upper arm for 5 hours and observed for up to 72 hours. The results are as shown in Table 4.
[0040]
[Table 4] Comparative test of inflammation suppressing effect
[0041]
(Example 7) Effect of skin cream for beautiful skin
Using the samples prepared in Example 6, skin creams having the compositions shown in Table 5 were prepared, and their effects and feeling of use were evaluated according to the following methods.
[Production method]
The aqueous phase treated in the same manner as the B group component was added to the oil phase kept at 80 ° C. by heating and dissolving the A group component in Table 5, emulsified, and cooled to obtain products (skin creams 1 to 3).
[Evaluation method]
The product prepared as described above was evaluated by applying it to the face and hands of 20 subjects for each cream at a usage of 1 g / day of the product and using it for one month.
[0042]
[Table 5] Comparative evaluation of skin cream for beautiful skin
* 2 : Skin cream 2 (the present invention)
* 3 : Skin cream 3 (the present invention)
[0044]
As shown in Table 5, skin creams 2 and 3 to which the mixed extracts 1 and 2 of the present invention are added are more remarkably beautiful skin effects and uses than skin creams 1 to which the conventional method, Bidens-Pirosa water, is mixed. Improvement in feeling was observed. No one had sued for rash.
[0045]
(Example 8) Reduction of atopic dermatitis by emulsion
6 g of ginger was mixed with 94 g of Bidens-Pilosa, the same as in Example 1, 2 L of ethanol was added, and the mixture was extracted at room temperature for 7 days. Separately, mixed extract 4 was produced using 1,3-butylene glycol instead of ethanol. As a control, 100 g of Bidens-Pyrosa was similarly treated with ethanol or 1,3-butylene glycol (Bidence-Pyrosa + ethanol, and Bidence-Pyrosa + 1,3-butylene glycol).
[Emulsion manufacturing method]
The aqueous phase treated in the same manner as the B group component was added to the oil phase kept at 80 ° C. by heating and dissolving the A group component in Table 6, emulsified, and cooled to obtain products (emulsions 1 to 4).
[0046]
[Evaluation method]
Emulsions containing these extracts and having the composition shown in Table 6 were evaluated in atopic dermatitis patients (10 patients in total). 0.5g / cm at the site where atopic dermatitis has developed 2 It was used for about one month at the amount used. The effects and feeling of use were evaluated according to the following methods.
[Table 6] Improvement of atopic dermatitis by emulsion
As can be seen from the results in Table 6, the examples (mixed extracts 3 and 4) of the present invention were more comparable when extracted with ethanol and with 1,3-butylene glycol (Bidence Pyrosa + ethanol). , Bidens Pyrosa + 1,3-butylene glycol), a remarkable anti-inflammatory effect and an improvement in Kayumi-stopping effect were observed.
[0049]
(Example 9) Improvement of the effect of sunscreen cream
[Production method of sunscreen cream]
Ingredient A of Table 7 was heated and dissolved, and an aqueous phase treated in the same manner as Component B was added to the oil phase kept at 80 ° C. to emulsify and cool to obtain products (sun creams 1-4). . Note that, in Table 7, the mixed extract 3, the mixed extract 4, Bidens-Pyrosa + ethanol, and Bidens-Pyrosa + 1,3-butylene glycol are the same as those prepared in Example 8.
[Evaluation method]
The obtained product was applied to the back of a person and irradiated with UVB, and the sun protection effect SPF was calculated and evaluated according to the measurement method of the Japan Cosmetic Industry Association.
[0050]
[Table 7] Improvement of sunscreen cream effect
* 2 : Sunscreen cream 2 (the present invention)
* 3 : Sunscreen cream 3 (comparative example)
* 3 : Sunscreen cream 4 (the present invention)
[0051]
As can be seen from the results in Table 7, the examples (mixed extracts 3 and 4) of the present invention were compared (Bidence Pyrosa + ethanol) both when extracted with ethanol and when extracted with 1,3-butylene glycol. , Bidens Pyrosa + 1,3-butylene glycol), a remarkable improvement in the effect of preventing sunburn was observed.
[0052]
(Example 10)
After cutting and washing the above-ground part of the Tachiwa snow clover, it was chopped to an appropriate length, steamed for about 1 hour, and sent with hot air at 80 ° C. and dried by ventilation. To 10 kg thereof, 3 kg of dried ginger was added, and 200 L of a mixed solution of water / methanol / 1,3-butylene glycol (1/1/2, v / v) was added thereto. Was performed, and the insoluble matter on the filter paper was sufficiently squeezed to obtain a filtrate. 0.5 kg of lactose was added to the obtained filtrate and spray-dried to obtain 2.9 kg of extract powder.
0.3 kg of Avicel (crystalline cellulose) as an excipient and 0.1 kg of sucrose fatty acid ester as a lubricant were added to the extract powder, and the mixture was tableted to give tablets of 250 mg.
[0053]
Effects on cedar pollinosis
Five cedar pollinosis patients having symptoms such as sneezing, runny nose, and itchy eyes were used as subjects. All five had taken measures such as wearing antiallergic agents and masks under the guidance of a doctor, but their symptoms still did not ease. When the five subjects stopped the medicine of the doctor and took the tablets of the present invention three times a day three times a day on an empty stomach, the symptoms were clear within 10 days as shown below. lost. After that, she had no symptoms as long as she continued to take the drug, but she developed the disease if she missed taking it for a few days.
[0054]
1) Y. S. A man was 47 years old (company manager) and had almost no symptoms in 5 to 7 days, and did not recur.
2) S.I. T. Symptoms became very light in about a week, a man 39 years old (company sales clerk).
3) K. I. A male 44 years old (a local government employee) had almost no symptoms in 4 to 5 days, and did not recur thereafter.
4) M.P. I. The symptoms of hay fever almost disappeared a few days after a 75-year-old man (company officer).
5) R.I. I. A woman 39 years old (company clerk) 10 days later, her symptoms have become extremely light.
【The invention's effect】
As described above, the composition containing the extract obtained by the method of the present invention has excellent effects as a therapeutic and preventive agent for hyperglycemia, dermatitis, hay fever and the like, and as a cosmetic agent such as a skin beautifying agent and sunscreen. In addition, it shows a clearly superior effect than the extract of Bidens pirosa alone.
Claims (19)
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| JP2006306769A (en) * | 2005-04-27 | 2006-11-09 | Toyo Hakko:Kk | Apoptosis inducing agent, food and drink and medicine containing the same, and method for producing the apoptosis inducing agent |
| JP2007197380A (en) * | 2006-01-27 | 2007-08-09 | Musashino Meneki Kenkyusho:Kk | Anti-histaminic agent comprising enzyme-treated product of plant belonging to genus bidens |
| WO2007113698A3 (en) * | 2006-03-03 | 2008-01-03 | Csir | Preventative treatment and remission of allergic diseases |
| WO2008018138A1 (en) | 2006-08-10 | 2008-02-14 | Wood One Co., Ltd. | Hypoglycemic composition containing component originating in the bark of tree belonging to the genus acacia |
| US8124138B2 (en) | 2006-08-10 | 2012-02-28 | Mimozax Co., Ltd. | Composition for prevention and/or treatment of pruritus containing acacia bark derivative |
| US8124137B2 (en) | 2006-08-10 | 2012-02-28 | Mimozax Co., Ltd. | Composition for prevention and/or treatment of tumors containing acacia bark derivative |
| KR101181797B1 (en) * | 2009-12-09 | 2012-09-17 | 노용수 | Method for Preparing Fermented Liquor of Epicarp of Ginkgo Biloba |
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| WO2015167545A1 (en) * | 2014-04-30 | 2015-11-05 | Kimberly-Clark Worldwide, Inc. | Use of bidens extract to reduce signs of skin aging |
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| CN109984963A (en) * | 2019-05-13 | 2019-07-09 | 烟台新时代健康产业日化有限公司 | A kind of preparation method of the anti-oxidant skin care item of pollen pini-turmeric |
| US10548835B2 (en) | 2014-04-30 | 2020-02-04 | Kimberly-Clark Worldwide, Inc. | Methods of reducing the signs of skin aging |
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| JP2006306769A (en) * | 2005-04-27 | 2006-11-09 | Toyo Hakko:Kk | Apoptosis inducing agent, food and drink and medicine containing the same, and method for producing the apoptosis inducing agent |
| JP2007197380A (en) * | 2006-01-27 | 2007-08-09 | Musashino Meneki Kenkyusho:Kk | Anti-histaminic agent comprising enzyme-treated product of plant belonging to genus bidens |
| US8158165B2 (en) | 2006-03-03 | 2012-04-17 | Csir | Preventative treatment and remission of allergic diseases |
| WO2007113698A3 (en) * | 2006-03-03 | 2008-01-03 | Csir | Preventative treatment and remission of allergic diseases |
| US9132159B2 (en) | 2006-08-10 | 2015-09-15 | Mimozax Co., Ltd. | Composition for prevention and/or treatment of tumors containing acacia derivative |
| US8124137B2 (en) | 2006-08-10 | 2012-02-28 | Mimozax Co., Ltd. | Composition for prevention and/or treatment of tumors containing acacia bark derivative |
| US8128969B2 (en) | 2006-08-10 | 2012-03-06 | Mimozax Co., Ltd. | Hypoglycemic composition containing acacia bark derivative |
| US8124138B2 (en) | 2006-08-10 | 2012-02-28 | Mimozax Co., Ltd. | Composition for prevention and/or treatment of pruritus containing acacia bark derivative |
| US8673287B2 (en) | 2006-08-10 | 2014-03-18 | Mimozax Co., Ltd. | Anti-obesity composition containing acacia bark derivative |
| WO2008018138A1 (en) | 2006-08-10 | 2008-02-14 | Wood One Co., Ltd. | Hypoglycemic composition containing component originating in the bark of tree belonging to the genus acacia |
| KR101181797B1 (en) * | 2009-12-09 | 2012-09-17 | 노용수 | Method for Preparing Fermented Liquor of Epicarp of Ginkgo Biloba |
| GB2541319A (en) * | 2014-04-30 | 2017-02-15 | Kimberley-Clark Worldwide Inc | Use of bidens extract to reduce signs of skin aging |
| WO2015167552A1 (en) * | 2014-04-30 | 2015-11-05 | Kimberly-Clark Worldwide, Inc. | Topical compositions including a bidens extract for reducing signs of skin aging |
| WO2015167545A1 (en) * | 2014-04-30 | 2015-11-05 | Kimberly-Clark Worldwide, Inc. | Use of bidens extract to reduce signs of skin aging |
| GB2541319B (en) * | 2014-04-30 | 2019-04-24 | Kimberly Clark Co | The non-therapeutic use of a combination of a bidens extract and an undaria extract |
| US10548835B2 (en) | 2014-04-30 | 2020-02-04 | Kimberly-Clark Worldwide, Inc. | Methods of reducing the signs of skin aging |
| US10646430B2 (en) | 2014-04-30 | 2020-05-12 | Kimberly-Clark Worldwide, Inc. | Topical compositions for stimulating adipogenesis and lipogenesis to reduce the signs of skin aging |
| GB2541317B (en) * | 2014-04-30 | 2020-06-03 | Kimberly Clark Co | Methods of reducing signs of skin aging |
| US11154491B2 (en) | 2014-04-30 | 2021-10-26 | Kimberly-Clark Worldwide, Inc. | Use of Undaria extract to reduce signs of skin aging |
| US11260020B2 (en) | 2014-04-30 | 2022-03-01 | Kimberly-Clark Worldwide, Inc. | Topical compositions and methods for reducing oxidative stress |
| CN109984963A (en) * | 2019-05-13 | 2019-07-09 | 烟台新时代健康产业日化有限公司 | A kind of preparation method of the anti-oxidant skin care item of pollen pini-turmeric |
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