JP4028837B2 - Cosmetics - Google Patents

Description

  The present invention relates to cosmetics that exhibit excellent whitening and skin-beautifying action on skin and are useful as basic cosmetics, makeup cosmetics, bath preparations and the like.

  Various whitening agents have been proposed and formulated for the purpose of preventing or improving pigmentation caused by sunburn or aging, especially spots and freckles, and maintaining the skin in a youthful and healthy state. Cosmetics that have been put on the market. However, with these conventional whitening agents, it is difficult to sufficiently satisfy both of the whitening / skin-beautifying effect and skin safety, and there is a need for new cosmetics containing whitening agents with improved such points. .

  In light of the problems of the prior art, the present inventors have conducted intensive research to find a new whitening active ingredient derived from a natural product from the viewpoint of skin safety. It has been found that it has a remarkable tyrosinase activity inhibitory action, and that this makes it possible to provide a cosmetic with excellent whitening / skin-beautifying effect and skin safety, thus completing the present invention.

  Physiological activity of the plant belonging to the genus Ceraminaceae includes, for example, the roots of Codonopsis pilosula (herbal medicine “participant”), symptom improvement effect such as gastrointestinal weakness and loss of appetite, hair growth effect or sebaceous gland function activation effect, etc. However, it has been revealed for the first time after the present invention that there is no known knowledge that the extract of the plant of the genus Turrundin has a remarkable tyrosinase activity-inhibiting action.

JP-A-2-48515 JP-A-61-200922 Chuyaku Dictionary, Volume 3, page 1909 (Shōgakukan, 1985)

That is, the present invention is a skin cosmetic (except for those applied to the head), characterized in that it contains an extract of Codonopsis pilosula of a plant belonging to the genus Zucarinaceae .
In the present specification, the term cosmetic is used in a broad sense including so-called cosmetics and quasi drugs.

The cosmetic composition of the present invention, which is composed of an extract of the genus Chrysanthaceae genus genus carrot , has a remarkable tyrosinase activity inhibitory action exhibited by the extract, which is contained as an active ingredient, and is prominent in skin pigmentation such as spots and buckwheat. In addition, since the extract is derived from a natural product, there is little irritation to the skin and the safety is excellent.

Hereinafter, the present invention will be described in detail.
The key Kyo family codonopsis of plants, such as shade Roh Codonopsis (Codonopsis pilosula), diisobutyrate (Codonopsis laroceolata), Baasobu (Codonopsis ussuriensis), Tsubu kava carrot (Codonopsis lanceolata) but the like, in the present invention , among them, in view of tyrosinase activity inhibiting effect of the extract obtained, more shade Roh Codonopsis it is used in terms of easiness of availability of raw materials.

When preparing the extract of key kyou family codonopsis plants it is not particularly limited to the extraction site of the plant, such as seeds, leaves, stems, roots, but may be any appropriate portion, such as whole plant, among others The use of roots is most preferred, and particularly good results are obtained when using roots of lacquer carrot.

  The extract is prepared by washing the water-carrying plant in advance with water if necessary to remove foreign substances, and then leaving it as it is or drying, and then chopping or crushing as necessary, soaking, countercurrent extraction It can be performed by contacting with an extraction solvent according to a conventional method such as a method.

  Here, the extraction solvent is water; lower alcohols such as methanol, ethanol and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol and glycerine; ethyl acetate, butyl acetate and methyl propionate. Esters such as; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene and chloroform, etc., which may be used alone or in combination of two or more Used.

  Among these extraction solvents, water, lower alcohols or polyhydric acids are used in the present invention from the viewpoint of the tyrosinase activity-suppressing action of the extract obtained and from the viewpoint that it can be widely applied to cosmetics. Hydrophilic solvents such as alcohols are preferably used. Preferred examples of using this hydrophilic solvent include, for example, water or lower alcohols (especially ethanol) used alone, or a mixed solvent of water and lower alcohols (especially ethanol) or water and polyhydric alcohols (especially Use of a mixed solvent with 1,3-butylene glycol or propylene glycol) is exemplified, and water alone is most preferable.

  In the case of using a mixed solvent, the mixing ratio of each solvent is, for example, 1: 1 to 10 in terms of weight ratio as long as it is a mixed solvent of water and ethanol, water and 1,3-butylene glycol, or water and propylene glycol. : It is preferable to set it as the range of about 1.

  In preparing the extract, there is no particular limitation on the pH of the extract, but generally it is preferably in the range of 4-8. In this sense, if necessary, the extraction solvent may be sodium hydroxide, sodium carbonate, water. You may mix | blend alkaline adjusters, such as potassium oxide, acidic adjusters, such as a citric acid, hydrochloric acid, phosphoric acid, and a sulfuric acid, and you may adjust so that it may become desired pH.

  Extraction conditions such as extraction temperature, extraction time, and bath ratio vary depending on the type and pH of the solvent used, or the extraction site and shredding degree of the plant, but for example, in the case of the immersion method using water as the extraction solvent The extraction temperature is generally in the range of 1 to 80 ° C., preferably 2 to 40 ° C., and the extraction time is 12 hours to 10 days in the case of cold extraction at 4 ° C., particularly 24 hours to 7 days. In some cases, it ranges from 6 hours to 7 days, in particular from 24 hours to 4 days, in the case of high temperature extraction at 80 ° C., from 30 minutes to 2 hours, in particular from 1 hour to 6 hours. In the case of the dipping method, the bath ratio is a weight ratio, and the solvent is generally 5 to 50 times, preferably 10 to 30 times the amount of the plant body.

  The extract solution thus obtained is generally adjusted to a pH of 4 to 8, and may be used as it is as a cosmetic compounding agent, or may be used at an appropriate concentration by vacuum concentration or the like if necessary. . Further, depending on the case, it can be pulverized according to a conventional method such as spray drying or freeze drying.

  As described later in Test Examples 1 to 5, the extract of the genus Oleaceae genus plant of the present invention prepared as described above has a remarkable tyrosinase activity direct inhibitory action, intracellular tyrosinase activity inhibitory action, It has an inhibitory effect on melanin production, is less irritating to the skin, has excellent biological safety, prevents the occurrence of spots and freckles, or improves their symptoms to maintain the skin in a youthful and healthy state. It is extremely useful for blending into cosmetics for the purpose. Therefore, according to the present invention, there is provided a whitening and skin-beautifying cosmetic containing an extract of the plant belonging to the genus Ceraminaceae.

  Examples of cosmetics containing the extract of the genus Cultivation plant of the present invention include basic cosmetics such as milky lotion, cream, lotion, essence, pack, lipstick, foundation, liquid foundation, makeup press powder, cheek red, white powder Makeup cosmetics such as facial cleansers, body shampoos, soaps and other cleaning cosmetics, and bath agents, but of course are not limited thereto.

  In the cosmetics of the present invention, the amount of the plant of the genus Ceraminaceae is generally 0.002 to 1.0% by weight, preferably 0.02 in the case of basic cosmetics, as the solid content of the extract. In the case of makeup cosmetics, generally in the range of 0.002 to 1.0% by weight, preferably in the range of 0.02 to 0.2% by weight. In general, it is in the range of 0.002 to 10.0% by weight, preferably 0.02 to 7.0% by weight.

  The cosmetics of the present invention include components that are usually used in cosmetics, such as oily components, surfactants (synthetic systems, natural products), moisturizers, sensitizers, in addition to the essential components of the plant belonging to the genus Ceraminaceae. A sticking agent, an antiseptic / bactericidal agent, a powder component, an ultraviolet absorber, an antioxidant, a pigment, a fragrance and the like can be appropriately blended as necessary. In addition, other physiologically active ingredients may be added to the cosmetic composition as long as the effectiveness and features of the asteraceae genus plant extract of the present invention are not impaired.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.
In addition, as emulsifiers or emulsifiers, stevia derivatives such as enzyme-treated stevia, lecithin and its derivatives, lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, legumes, millet, etc.), juteiro (Rhamnaceae zizyphus joazeiro) An extract or the like can also be blended.

  Examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, and sugars such as trehalose, lactic acid bacteria fermented rice, and mucopolysaccharides. (For example, hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, beech extract Products, seafood-derived collagen and derivatives thereof, various amino acids and derivatives thereof.

  Examples of thickeners include, for example, brown algae such as alginic acid, agar, carrageenan, fucoidan, green algae or red algae-derived components, extract of sand cucumber, pectin, locust bean gum, polysaccharides such as aloe polysaccharide, xanthan gum, tragacanth gum, guar gum, etc. Synthetic polymers such as gums, cellulose derivatives such as carboxymethylcellulose, hydroxyethylcellulose, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives, polyglutamic acid and its derivatives, etc. Is mentioned.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzal chloride Luconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), 1,2-pentanediol, various essential oils, bark dry matter, and the like.

  Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powders, powders of cereals (rice, wheat, corn, millet, etc.), powders of beans (soybeans, red beans, etc.).

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives, beech extract, rice extract and the like.

  Examples of physiologically active ingredients include whitening ingredients such as t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone derivatives, ellagic acid and derivatives thereof, resorcinol derivatives, sucha akuhi extract, yukinoshita extract, rice bran Extract, rice bran extract hydrolyzate, lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented cereals (barley, beans, millet), white coconut hydrolyzed extract, murasakixikibu extract, Pandanus amaririfolios (Pandanus amaryllifolius Roxb.) extract, Arcangelicia flava Merrilli extract, chamomile extract (trade name: chamomile ET), seaweed extract such as kombu, seaweed extract such as sea bream, linoleic acid and Derivatives or processed products thereof (eg liposomal linoleic acid), 2,5-dihydro Xylbenzoic acid derivatives and the like, and as skin aging preventing / beautifying components, animal or fish-derived collagen and derivatives thereof, elastin and derivatives thereof, nicotinic acid and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salt, etc.), t -Cycloamino acid derivatives, vitamin A and derivatives thereof, vitamin E and derivatives thereof, allantoin, α-hydroxy acids, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, pearl extract, chamomile extract, Herbal extract such as carrot extract, aloe extract, rice extract hydrolyzate, rice bran extract hydrolyzate, rice fermentation extract, beetroot extract, anaaaosa extract, seaweed extract such as eelgrass, sohakuhi extract, juazeiro ( Rhamnaceae zizyphus joazeiro ) There are extracts.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-O-α) -D-glucopyranosyl-L-ascorbine Acid) ascorbic acid sugar derivatives, acylated 6-positions of these ascorbic acid sugar derivatives (acyl groups are hexanoyl, octanoyl, decanoyl, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetra L-ascorbic acid tetrafatty acid esters such as lauric acid ester, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium and the like are hydroquinone derivatives such as arbutin (hydroquinone). -Β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like, and as resorcinol derivatives, for example, 4-n-butylresorcinol, 4-isoamylresorcinol and the like are 2,5-dihydroxybenzoic acid. Derivatives and For example, 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, etc., and nicotinic acid derivatives include, for example, nicotinic acid amide, nicotinic acid benzyl, etc. However, examples of the vitamin E derivative include vitamin E nicotinate and vitamin E linoleate, and examples of the α-hydroxy acid include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

  Next, the present invention will be described more specifically with reference to production examples, examples (formulation examples), and test examples, but the present invention is not limited thereto. In the following, all parts are by weight, and all% are by weight.

Production Example 1 Preparation of a plant extract solution (1)
1000 g of purified water was added to 100 g of dried shredded roots of Codonopsis pilosula and extracted at 4 ° C. for 12 hours. The obtained extract was filtered to obtain 875 g of a pale yellow transparent extract solution (solid content concentration: 2.46%).

Production Example 2 Preparation of extract of genus plant of the genus Cultivation (2)
1000 g of purified water was added to 100 g of dried chopped carrot root and extracted at 80 ° C. for 1 hour. The obtained extract was filtered to obtain 925 g of a pale yellow transparent extract solution (solid content concentration: 2.60%).

Production Example 3 Preparation of the extract of the plant of the genus Cultivation (3)
1000 g of a 30% 1,3-butylene glycol aqueous solution was added to 100 g of dried shredded whole plant of lacquer carrot and extracted at 80 ° C. for 1 hour. The obtained extract was filtered to obtain 880 g of a yellowish brown transparent extract solution (solid content concentration: 2.08%).

Production Example 4 Preparation of the plant extract solution (4)
1000 g of a 50% ethanol aqueous solution was added to 100 g of dried chopped roots of sagenut carrot (Salicornea herbacea) and extracted at 4 ° C. for 12 hours. The obtained extract was filtered to obtain 918 g of a pale yellow transparent extract solution (solid content concentration: 1.92%).

Production Example 5 Preparation of the plant extract solution
100 ml of the extract solution obtained in the same manner as in Production Example 1 was concentrated to 10 ml and then freeze-dried to obtain 2.4 g of extract powder.

Reference production example . Preparation of the plant extract solution
In Production Example 1, a yellow transparent extract solution (solid content concentration: 2.79%) was obtained in the same manner as in Production Example 1 except that diisobute (Condonopsis laroceolata) roots were used instead of the root of carrot root carrot. 890 g was obtained.

Example 1. Cream [Component A] Liquid paraffin 5.0
Hexalan (Note 1) 4.0
Paraffin 5.0
Glyceryl monostearate 2.0
Polyoxyethylene (20) sorbitan monostearate 6.0
Butylparaben 0.1
(Note 1) Technoble Co., Ltd. glyceryl trioctanoate
[B component]
Extract solution of Production Example 1 5.0
Glycerin 5.0
Carboxymethyl monostearate 0.1
Moiston C (Note 2) 1.0
Amount of purified water totaling 100 parts (Note 2) NMF component manufactured by Technoble Co., Ltd.
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After this was cooled to 50 ° C., component C was added and further stirred and mixed to obtain a cream.

Example 2 Cream A cream was obtained in the same manner as in Example 1 except that the extract solution of Production Example 2 was used instead of the extract solution of Production Example 1 in the component B of Example 1.

Example 3 Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Extract solution of Production Example 1 5.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 4 Lotion [component] part Extract solution of Production Example 3 5.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total
The above ingredients were mixed to obtain a lotion.

Example 5 FIG. Lotion [component A] part olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[B component]
Extract solution of Production Example 4 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total
[C component]
Perfume
After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes.
After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Example 6 Emulsion An emulsion was obtained in the same manner as in Example 3 except that the extract solution of Production Example 2 was used instead of the extract solution of Production Example 1 in the component B of Example 3.

Example 7 Emulsion An emulsion was obtained in the same manner as in Example 3 except that the extract solution of Production Example 3 was used instead of the extract solution of Production Example 1 in the component B of Example 3.

Example 8 FIG. (Reference Example) Emulsion An emulsion was obtained in the same manner as in Example 3 except that the extract solution of Reference Production Example was used instead of the extract solution of Production Example 1 in the component B of Example 3.

Example 9 Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monosteare 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Extract solution of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Production Example 10 Emulsion Other than using 2.0 parts of L-ascorbic acid-2-phosphate magnesium in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Example 9 Obtained an emulsion in the same manner as in Example 9.

Example 11 Emulsion In addition to using 2.0 parts of L-ascorbic acid-2-phosphate and 2.0 parts of sodium L-ascorbic acid-2-phosphate in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Example 9 Obtained an emulsion in the same manner as in Example 9.

Example 12 Emulsion In the component B of Example 9, the emulsion was prepared in the same manner as in Example 9, except that 2.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. Obtained.

Example 13 Latex In the component B of Example 9, instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide, a rice bran extract hydrolyzate (trade name “Grace Snow” manufactured by Technoble Co., Ltd.) An emulsion was obtained in the same manner as in Example 9, except that 5.0 parts of * Snow * HP ", solid concentration 3.5%) were used.

Example 14 Emulsion In the component B of Example 9, in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide, white coconut extract (trade name “Sinablanca-WH” manufactured by Technoble Co., Ltd.) A solid emulsion was obtained in the same manner as in Example 9, except that 5.0 parts of a solid content concentration of 1.0% was used.

Example 15. Emulsion Example 9 except for using 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the component B of Example 9 except that 1.0 part of γ-amino-β-hydroxybutyric acid is used. In the same manner as in No. 9, an emulsion was obtained.

Example 16 Pressed powder [A component] Part Bengala 0.5
Yellow iron oxide 1.5
Black iron oxide 0.1
Titanium oxide 10.0
Nylon powder 4.0
Amount that makes 100 parts of sericite Mica 23.0
Talc 25.0
Extract powder of Production Example 5 0.1
[B component]
Squalane 1.0
Methylpolysiloxane 4.0
Propylparaben 0.1
Dehydroacetic acid 0.1
Liquid paraffin 2.0
Perfume
The above A component and B component were mixed and agitated, mixed, then passed through a 200 mesh Tyler mesh sieve, and the resulting mixed powder was cast into a mold to obtain a pressed powder.

Example 17. Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B component]
Extract solution of Production Example 2 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [C component]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Example 18 Cream foundation [component A] part Stearic acid 5.0
Cetanol 2.0
Glyceryl monostearate 3.0
Liquid paraffin 5.0
Squalane 3.0
Isopropyl myristate 8.0
Polyoxyethylene (20) glyceryl monostearate 2.0
Propylparaben 0.1
[B component]
Extract solution of Production Example 3 5.0
Sorbitol 3.0
1,3-butylene glycol 5.0
Triethanolamine 1.5
Methylparaben 0.1
Purified water Amount of 100 parts [component C]
Titanium oxide 8.0
Talc 2.0
Kaolin 5.0
Bentonite 1.0
Coloring pigment appropriate amount [component D]
Fragrance 0.3
Component C was mixed and pulverized with a pulverizer. The component B was mixed, and the pulverized component C was added thereto and uniformly dispersed in a colloid mill. The components A and B and C dispersed uniformly were each heated to 80 ° C., and then the components A were added to the components B and C while stirring, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, D component was added and stirred and mixed, and also it cooled to 30 degrees C or less, stirring, and obtained the cream foundation.

Example 19. Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B component]
Extract solution of Production Example 3 5.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Example 20. Soap [component A] part hardened castor oil 26.0
Coconut oil 10.0
Olive oil 4.0
[B component]
Sodium hydroxide 6.0
Sugar 10.0
Glycerin 5.0
Extract powder of Production Example 5 0.1
Purified water Amount of 100 parts [component C]
Ethanol 20.0
Perfume Appropriate A component and B component were each heated to 80 ° C. and dissolved uniformly, and then B component was added to A component to saponify. This was cooled to 50 ° C. with stirring, and component C was added. This was poured into a mold and cooled, and then dried at room temperature for several days. A sufficiently dried product was taken out from the mold to obtain soap.

Comparative Example Cream A cream was prepared in the same manner as in Example 1 except that 2.0 parts of arbutin was used in place of 5.0 parts of the plant extract of the genus Genus of Production Example 1.

Control Example Cream A cream was prepared in the same manner as in Example 1 except that purified water was used in place of the plant extract of the genus Genus in Production Example 1.

Test Example 1 Direct inhibitory effect on tyrosinase activity Using tyrosinase derived from mushrooms, the direct inhibitory effect on the tyrosinase activity of the plant of the genus Turnin was examined.
[Test method]
1.0 mL of the extract solution of Production Example 2 was mixed with 0.9 mL of McIlvain buffer, 1.0 mL of 0.03% L-tyrosine solution, and mushroom-derived tyrosinase solution, reacted at 37 ° C. for 30 minutes, and absorbance at a wavelength of 475 nm. Was measured.
For comparison, the same test was carried out when 1 mM arbutin solution was added instead of the extract solution of Production Example 2 (positive control) and when no sample was added (blank).

[Test results]
The results are shown in Table 1.

From the results of Table 1, it can be seen that the extract of the genus plant of the present invention has a remarkable tyrosinase activity direct inhibitory action.
Arbutin used as a positive control also significantly inhibited tyrosinase activity, confirming that the test system was normal.

Test Example 2 Inhibition of intracellular tyrosinase activity [Test method]
Cultured B16 mouse melanoma cells were seeded at 8 × 10 ³ cells / well in a 96-well microplate, and were subjected to 1 under conditions of 37 ° C. and 5% CO 2 in Eagle's minimum essential medium (MEM) containing 10% calf serum (FBS). After pre-culture for a day, the extract solution of Production Example 1 was replaced with a solution diluted to a concentration of 2.5 or 5.0% (as a solution) with Eagle MEM containing 10% FBS. Cultured for days.
Next, the culture solution is removed, and 0.3 mg / mL MTT solution is added, or a surfactant (Triton X-100) and 5 mM L-dopa solution are added and reacted at 37 ° C., and then microplate Using a reader (Model 450, manufactured by Bio-Rad), the MTT value was measured at 570-630 nm, and the dopa value was measured at a wavelength of 490 nm.
For comparison, the same test was performed when 3 mM arbutin was added instead of the extract solution of Production Example 1 and when no sample was added (blank).

[result]
The results obtained in the above test are shown in Table 2.

From the results in Table 2, it can be seen that the extract of the genus plant of the present invention significantly suppresses the intracellular tyrosinase activity without being accompanied by a decrease in the cell activity.
Arbutin used as a positive control also significantly inhibited tyrosinase activity, confirming that the test system was normal.

Test Example 3 Intracellular melanin synthesis inhibition test [Test method]
Cultured B16 melanoma cells were seeded at 5.0 × 10 ⁵ in a flask, pre-cultured in 10% FBS-containing Eagle MEM at 37 ° C. under 5% CO ₂ , and then 10% FBS-containing Eagle MEM. The extract solution of Production Example 2 was replaced with a solution diluted to a concentration of 5.0% (as a solution) and cultured under the same conditions for 3 days.
Next, the culture solution was removed and the cells were collected, and then the cell contents were extracted by adding 0.1N NaOH-containing 10% DMSO solution. For this extract, the amount of melanin was measured at a wavelength of 475 nm using a spectrophotometer (U-2000, manufactured by Hitachi, Ltd.), and the amount of protein was measured using a protein assay kit (manufactured by Bio-Rad).
From the results obtained here, the amount of melanin per amount of protein was calculated, and the relative value of the amount of melanin when the sample was added when the amount of melanin in the control was 100 was expressed as the melanin synthesis rate.
For comparison, the same test was carried out when a 3 mM arbutin solution was added instead of the extract solution of Production Example 2 and when no sample was added (blank).

[result]
The results obtained in the above test are shown in Table 3.

From the results in Table 3, it can be seen that the extract of the genus plant of the present invention significantly suppresses intracellular melanin synthesis.
Note that arbutin used as a positive control also significantly inhibited melanin synthesis, confirming that the test system was normal.

Test Example 4 Skin irritation
[sample]
(1) The extract solution obtained in Production Example 1 is kneaded with JP-hydrophilic petrolatum to a concentration of 5% (sample of the present invention)
(2) JP Hydrophilic Petrolatum (control)

[Test method]
5 adult males aged 20 to 50 years old as test subjects, the inner side of each upper arm was wiped with ethanol to remove sebum, and 0.2 g of each sample was applied to the aluminum plate of the fin chamber. Was affixed. After 24 hours, the fin chamber was removed, and the degree of skin irritation was determined by the method and criteria described below.
[Judgment]
After 1 hour, 24 hours and 48 hours after removing the patch, the state of erythema and edema at the applied site was visually determined based on the following “skin irritation criteria by dreze method”, and the average value of 5 subjects was calculated. Asked.
(Erythema)
Score skin condition 0: No erythema 1: Extremely mild erythema 2: Clear erythema 3: Moderate to strong erythema 4: Light crust formation in deep crimson erythema (edema)
Score skin condition 0: No edema 1: Extremely mild edema 2: Clear edema (distinguishable from surroundings)
3: Moderate edema (swelling of 1 mm or more)
4: Strong edema (further spread around)

[result]
The results are shown in Table 4.

  As is apparent from the results in Table 4, the extract of the plant of the genus Cultivation has almost no irritation to the skin and is extremely excellent in biological safety.

Test Example 5 Monitor Test For each cream of Example 1, Comparative Example and Control Example, the inhibitory effect on skin pigmentation and skin irritation were examined by a monitor test.
[Test method]
Twenty randomly selected women aged 18 to 50 years were used as subjects, and each cream was applied to the left upper arm twice a day (morning and evening) for 1 month. The erythema of the skin was visually observed and evaluated based on the following evaluation criteria.

[Evaluation criteria]
(Pigmentation)
A: No more B: Clearly less C: Some less D: Little change E: More (red spot)
A: No difference from the control example B: Little difference from the control example C: Some erythema is noticeable compared to the control example D: Equivalent erythema is noticeable compared to the control example E: Erythema is clearly seen compared to the control example stand out

[result]
The results are shown in Table 5.

  As shown in Table 5, the cream containing the extract of the genus plant of the present invention has an excellent anti-pigmentation effect, and has little irritation to the skin and high safety.

Claims (3)

Skin cosmetics (excluding those applied to the head) characterized by blending an extract of Codonopsis pilosula , a plant belonging to the genus Ceraminaceae . The skin cosmetic according to claim 1, wherein the root of carrots carrot is used. The skin cosmetic according to claim 1 or 2 , which is used for whitening.