JP6510744B2 - Cosmetics - Google Patents

Description

  The present invention is a cosmetic which is obtained from an apricot (Prunus armeniaca Linne var anus Maximowicz) or a phonon (Prunus armeniaca Linne) belonging to the genus Rosaceae, has excellent biosafety and is excellent in skin care and whitening effects. The present invention relates to a compounding component and a cosmetic obtained by compounding such a component.

  Skin aging is triggered by internal factors such as inactivation of cell proliferation and differentiation with age, reduction in hormone secretion, and reduction in the amount of extracellular matrix components, as well as sunlight (ultraviolet light) and various chemical substances. Damage to cells and tissues, or external factors such as inflammation. Aging of the skin is manifested as wrinkles, formation of tarmi, reduction of elasticity of the skin, stains, buckwheat, liver spots and the like.

  In order to prevent the aging of the skin and to keep the skin in a healthy and youthful state, conventionally, use of various active ingredients has been proposed, and a cosmetic containing such active ingredients (skin care ingredients, whitening ingredients, etc.) It is marketed. For example, antioxidants such as vitamin C, vitamin E, superoxide dismutase (Superoxide dismutase (hereinafter abbreviated as SOD), catalase); anti-inflammatory agents such as glycyrrhizinic acid; various ultraviolet light absorbers; α-hydroxycarboxylic acid, placenta extract Cell activating components such as γ-amino-β-hydroxybutyric acid; Extracellular matrix components such as collagen, elastin, hyaluronic acid; Humectants such as urea. In addition, arbutin, kojic acid and the like are known as substances that suppress the occurrence of pigmentation such as skin stains, buckwheat and melasma, and are widely used as active ingredients of skin lightening agents.

  As described above, conventionally, a skin-refining agent and a skin-whitening agent have been proposed based on various mechanisms of skin aging, but the safety for the skin, etc. and the effectiveness when actually applied to the skin There is a problem in terms of perspective.

Furthermore, in recent years, cosmetic ingredients that are effective for preventing and improving damage to the skin due to various external environmental factors (ultraviolet rays, chemical substances, house dust, pollen) have been sought, but The ingredients that are satisfactory are not yet known.

  In view of the problems of the prior art, the present inventors have been able to protect skin from various external environmental factors with a beautifying component and a whitening component derived from natural products from the viewpoint of skin safety. I did my best research to find As a result, the extract of the anise (Prunus armeniaca Linne var anus Maximowicz) or Prunus armeniaca Linne of the Rosa family Sakura genus has anti-inflammatory action and primary skin irritation suppressing action, as well as antioxidant action, and their synergy By the action, it is possible to provide a cosmetic having excellent skin-care and whitening effects by blending the extract and having a skin-damage preventing and ameliorating effect caused by external environmental factors. Found out.

  Conventionally, head cosmetics (patent document 1) which blended the apricot extract of Rosaceae Sakura genus, a protease inhibitor (patent document 2), a bath agent (patent document 3), an antiallergic agent (patent document 4) Proposed.

Unexamined-Japanese-Patent No. 03-188013 gazette Japanese Patent Application Laid-Open No. 10-338642 Japanese Patent Application Laid-Open No. 2000-186031 JP-A-2001-064192

  However, conventionally, the extract of apricot has anti-inflammatory action, primary skin irritation suppressing action, and antioxidant action, and by their synergetic action, it is possible to have an excellent skin-care effect and whitening effect by blending the extract. It has not been known that it has and the effect of preventing and ameliorating skin damage caused by external environmental factors.

The present invention is a cosmetic comprising an extract of an anise (Prunus armeniaca Linne var anus Maximowicz) or an extract of a horse mackerel (Prunus armeniaca Linne) as an active ingredient.
In the present invention, it is preferable to use apricot fruit.
In the present specification, the term "cosmetic" is used in a broad sense including so-called cosmetics as well as quasi drugs.

The present invention is a cosmetic comprising, as an active ingredient, an extract of an apricot (Prunus armeniaca Linne var anus Maximowicz) or a phophorus (Prunus armeniaca Linne), which is a plant of the Rosaceae cherry genus, as an active ingredient Has anti-inflammatory action (degranulation suppression) and primary skin irritation suppressing action, and antioxidant action, and their synergetic action, anti-aging effects such as wrinkles and skin on skin, improving effects on skin firmness and luster It is possible to provide a cosmetic which has an effect of preventing and improving pigmentation such as stains, buckwheat and liver spots, and further preventing and ameliorating skin damage caused by external environmental factors.

FIG. 1 is a figure which shows the degranulation inhibitory effect of the apricot extract concerning this invention. FIG. 2 is a view showing the DPPH radical scavenging effect of the apricot extract according to the present invention. FIG. 3 shows the SOD-like activity effect of apricot extract according to the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail.
The extract material used in the present invention is an apricot (Prunus armeniaca Linne var anus Maximowicz) or a phophorus (Prunus armeniaca Linne) belonging to the genus Rosa, which belongs to any cultivar (variety or subspecies or hybrid species). Also good. It may also be plum (Prunus salicina) or plum (Prunus mume), which is a relative plant of apricot.

  The material used in the present invention may be apricot, apricot, plum or plum whole grass, fruit, peel, leaf, flower part, stem, seed, root, etc., but whole grass or fruit or flower The use of is preferred. The collection time and size of the site of use of apricot, hon apricot, plum or plum are not particularly limited, and any size and collection time may be used.

  In preparation of the extract, first, the use site (eg whole grass, flower parts and / or fruits) of apricot, hon apricot, plum or plum is washed in advance if necessary to remove foreign matter, and then it is as it is or dried The above is optionally chopped or crushed or pasted and brought into contact with an extraction solvent to carry out extraction. Extraction can be performed by bringing the material into contact with an extraction solvent according to a conventional method such as immersion, but it is also possible to use supercritical extraction or steam distillation.

  Examples of extraction solvents include water; lower alcohols such as methanol, ethanol and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol and glycerin; ethyl acetate, butyl acetate, methyl propionate and the like Esters; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene and chloroform, etc., which may be used alone or in combination of two or more Used.

  Among these extraction solvents, water is also used in the present invention in view of the anti-inflammatory action, primary skin irritation suppressing action and antioxidant action of the extract obtained, and further, that it can be widely applied to cosmetics. Hydrophilic solvents such as lower alcohols or polyhydric alcohols are preferred. Preferred examples of using this hydrophilic solvent include, for example, water, lower alcohols (especially ethanol), single use of polyhydric alcohol (especially 1,3-butylene glycol), or water and lower alcohols The use of a mixed solvent with (especially ethanol) or a mixed solvent of water and a polyhydric alcohol (especially 1,3-butylene glycol, glycerin) may, for example, be mentioned, among which water alone or with water and 1,3 Particular preference is given to mixed solvents of -butylene glycol.

  The mixing ratio in the case of using a mixed solvent is, for example, a mixed solvent of water and 1,3-butylene glycol, a mixed solvent of water and ethanol in a volume ratio (same below) of 1: 1 to 20: 1. If it exists, the range of 1: 1 to 25: 1, and if it is a mixed solvent of water and glycerin, is preferably in the range of 1: 1 to 20: 1.

  Also, the weight ratio of the apricot material to the extraction solvent is preferably in the range of 1:50 to 50: 1, and more preferably in the range of 1: 1 to 1:35.

  The pH of the extract is not particularly limited in preparation of the extract, but in general, the range of 3 to 9 is preferable. In this sense, if necessary, an alkaline regulator such as sodium hydroxide, sodium carbonate, potassium hydroxide or the like, or an acidic regulator such as citric acid, hydrochloric acid, phosphoric acid, sulfuric acid or the like is added to the extraction solvent, It may be adjusted to have a pH of

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used, but, for example, when water or 1,3-butylene glycol or a mixed solution of water and 1,3-butylene glycol is used as the solvent The extraction temperature is preferably in the range of 0 ° C. to 90 ° C., more preferably in the range of 0 ° C. to 80 ° C., and the extraction time is preferably 1 to 168 hours (1 hour to 1 week). And more preferably in the range of 1 to 120 hours (1 hour to 5 days).

  The apricot may be subjected to a hydrolysis treatment, if necessary, prior to or in parallel with the extraction treatment of the present invention. As a result, the storage stability and the like of the apricot, hon-anzu, plum or plum extract may be improved, and the extract as a cosmetic composition may be used more effectively.

  When the extract is subjected to an enzymatic hydrolysis treatment, the enzyme may, for example, be a proteolytic enzyme such as actinase, papain or pepsin, a amylolytic enzyme such as glucoamylase, α-amylase or β-amylase, cellulase, hemicellulase or pectinase Although one or two or more selected from an enzyme group of any of fibrinolytic enzymes and lipolytic enzymes such as lipase may be used, one or more selected from each of these enzyme groups It is more preferable to use in combination the enzymes of

  The amount of the enzyme added is, for example, in the range of 0.01 to 10% by weight in total based on the solid content of apricot, hon apricot, plum or plum whole grass, or flower parts and / or fruits. Is more preferably in the range of 0.1 to 2.0% by weight.

  The extract prepared as described above generally has a pH adjusted to 4 to 8 and may be used as it is as a cosmetic compounding agent, or used as a desired concentration by vacuum concentration etc. You may. In addition, the extract may be dried by a conventional method such as spray drying.

  In addition, the extract prepared as described above may be stored under refrigeration for a certain period of time in order to enhance storage stability and the like, and then the supernatant may be used as a cosmetic ingredient.

  Examples of cosmetics (including quasi-drugs) containing extracts of apricot, apricot, plum or plum according to the present invention include, for example, emulsions, creams, lotions, essences, basic cosmetics such as packs, lipsticks, foundations, liquid foundations And make-up pressed powder, make-up cosmetic such as blusher and white powder, face wash, body shampoo, shampoo for hair, detergent for cleaning such as soap, hair-growth agent, and further bath agent etc. It is not limited to

  In the cosmetic composition of the present invention, the compounding amount of apricot, apricot, plum or plum extract as solid content of the extract is generally 0.002 to 1.0% by weight, preferably 0. In the range of 02 to 0.2% by weight, in the case of makeup cosmetics, generally in the range of 0.002 to 1.0% by weight, preferably 0.02 to 0.2% by weight, and in the case of cosmetic for cleaning Is generally in the range of 0.002 to 10.0% by weight, preferably 0.02 to 7.0% by weight.

  In the cosmetic composition of the present invention, in addition to the extract of essential ingredients apricot, apricot, plum or plum, components usually used for cosmetics, such as oily ingredients, surfactants (synthetic, natural products), moisturizing Agents, thickeners, emulsifiers or emulsifying aids, preservatives / bactericidal agents, powder components, ultraviolet light absorbers, antioxidants, dyes, perfumes, other physiologically active components, etc. can be appropriately blended. . In addition, as long as the effectiveness and features of the extract of apricot extract of the present invention are not impaired, it may be mixed with other physiologically active ingredients in cosmetics without any problem.

Here, as an oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadow foam oil, shea butter, tea tree oil, avocado oil, Fats and oils derived from plants such as macadamia nut oil and plant derived squalane; fats and oils derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax and lanolin; liquid paraffin, vaseline, paraffin wax, squalane etc Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, setanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, I Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

As the surfactant, for example, polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, poly Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkyl benzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Aniline such as polyoxyethylene alkyl ether phosphate, α-sulfonated fatty acid alkyl ester salt, polyoxyethylene alkyl phenyl ether phosphate Surfactants; quaternary ammonium salts, primary to tertiary fatty amine salts, trialkyl benzyl ammonium salts, alkyl pyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethyl imidazolinium salts, N , N-dialkylmorpholinium salts, cationic surfactants such as polyethylene polyamine fatty acid amide salts; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaines, N, N, N-trialkyl-N- Amphoteric surfactants such as alkylene ammonio carboxy betaine, N-acylamidopropyl-N ', N'-dimethyl-N'-beta-hydroxypropyl ammonio sulfobetaine, etc. can be used.

As the emulsifier or emulsification aid, stevia derivatives such as enzyme-treated stevia, lecithin and its derivatives, lactic acid bacteria fermented rice, lactic acid bacteria fermentation germinated rice, lactic acid bacteria fermented cereals (wheat, beans, miscellaneous grains, etc.) can be blended.

Humectants include, for example, glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate and the like, and further saccharides such as trehalose, mucopolysaccharides (for example, hyaluronic acid) Acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc., elastin and its derivatives, collagen and its derivatives, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white) Extracts, various amino acids and their derivatives are included.

As thickeners, for example, ingredients derived from brown algae such as alginic acid, agar, carrageenan, fucoidan, green algae or red algae; silane root (white) extracts; polysaccharides such as pectin, locust bean gum, aloe polysaccharides, xanthan gum, Gums such as tragacanth gum and guar gum; cellulose derivatives such as carboxymethyl cellulose and hydroxyethyl cellulose; synthetic polymers such as polyvinyl alcohol, polyvinyl pyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives; Glutamic acid and its derivative etc. are mentioned.

Examples of antiseptic and bactericidal agents include urea; parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate and butyl parahydroxybenzoate; phenoxyethanol, dichlorophen, hexachlorophene, chlorhexidine hydrochloride, benzal chloride These include, for example, ruconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazo dianil urea), 1,2-pentanediol, various essential oils, bark dry residue and the like.

As a powder component, for example, sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, magnesium oxide, zirconium oxide, barium sulfate, anhydrous silicic acid, mica, nylon powder, polyethylene powder, silk powder, cellulose There are powder of grain-based powder, powder of cereals (rice, wheat, corn, millet etc.), and powder of beans (soybean, red beans etc).

UV absorbers include, for example, ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (eg, vitamin E nicotinate, vitamin E linoleate, etc.), murasoxib extract, peony extract, silane root (white ) There is an extract etc.

As a skin lightening agent, t-cycloamino acid derivative, kojic acid and its derivative, ascorbic acid and its derivative, hydroquinone or its derivative, ellagic acid and its derivative, nicotinic acid and its derivative, resorcinol derivative, tranexamic acid and its derivative, 4 -Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), 4-HPB (rhodenool, 4- (4-hydroxyphenyl) -4-butanol)), hydroxybenzoic acid And their derivatives, vitamin E and its derivatives, α-hydroxy acids, AMP (adenosine monophosphate, adenosine monophosphate), and these may be blended alone or in combination.

  Examples of kojic acid derivatives include kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid esters such as kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. As an ascorbic acid derivative, for example, sodium L-ascorbic acid-2-phosphate, magnesium L-ascorbic acid-2-phosphate, sodium L-ascorbic acid-2-sulfate, L-ascorbic acid-2 Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, ascorbic acid sugar derivatives such as L-ascorbic acid-5-glucoside, 6-position acylated compounds of these ascorbic acid sugar derivatives (acyl group is Hexanoyl group, o L-ascorbic acid tetra fatty acid esters such as tanoyl group, decanoyl group, etc., L-ascorbic acid tetraisopalmitate ester, L-ascorbic acid tetralaurate ester, 3-O-ethyl ascorbic acid, L-ascorbic acid 2-phosphate 6-O-palmitate sodium, glyceryl ascorbic acid or its acylated derivative, ascorbic acid glucerin derivative such as bis glyceryl ascorbic acid, and as a hydroquinone derivative, arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like, and tranexamic acid derivatives such as tranexamic acid esters (eg, tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranematic acid derivative) And amido acid cetyl esters or salts thereof), amides of tranexamic acid (eg, tranexamic acid methyl amide), etc., and resorcinol derivatives such as 4-n-butyl resorcinol, 4-isoamyl resorcinol, etc. -As a dihydroxybenzoic acid derivative, for example, 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid and the like, and as a nicotinic acid derivative, for example, nicotinic acid amide Examples of the α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid and α-hydroxyoctanoic acid.

As a physiologically active ingredient, as a whitening component, for example, a placenta extract, a soak extract, a yukinoshi extract, a shiso extract, a rice bran extract or a hydrolyzate thereof, a white apricot extract or a hydrolyzate thereof, a white eggplant Fermented product, Peony extract or its hydrolyzate, Lactic acid bacteria fermented rice, Musa curet extract, Lotus seed extract or its hydrolyzate, Lotus seed fermented product, Ginseng extract, castor bean hydrolyzate, castor bean seed fermented product, royal jelly Fermented material, fermented sake, fermented product of Pandanus amaryllifolius (Pandanus amaryllifolius Roxb.), Extract of Arcangelicia flava (Arcangelicia flava Merrilli), extract of chamomile etc. are raised, coral grass extract as an anti-aging component , Extract of rice leaf or its hydrolysate, eggplant (water eggplant, long eggplant, Kamo eggplant, rice eggplant etc.) extract or its hydrolysis Dissolution, extract of seaweeds such as katammen-kirin, extract of marine flowering plants such as eelgrass, fermented soy milk, damask rose extract, jellyfish water, rice extract or its hydrolysate, rice fermented extract, germinated rice Extract or its hydrolyzate, germinated rice ferment, black bean extract or its hydrolyzate, linoleic acid and its derivative or processed product (eg liposomal linoleic acid etc.), animal or fish-derived collagen and its derivative, elastin And its derivatives, glycyrrhizinic acid and its derivatives (such as dipotassium salts), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract , Carrot extract, aloe extract, mitsuishi comb extract, lice extract, ana Reed extract, Juazeiro (Zizyphus joazeiro) have extracts.

  Next, the present invention will be described more specifically by Production Examples, Formulation Examples and Test Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight and all% mean weight%.

Production Example 1 Preparation of Extract Solution of Hong Apricot Fruit The peels and seeds were removed from the fruit of the apricot sage Genus Hong apricot and made into a paste with a grinder. After adding 210 g of 1, 3- butylene glycol to 90 g of this fruit paste, it extracted at 4 degreeC. To this, 410 g of purified water was added, followed by filtration to obtain 587 g of a brown clear honander fruit extract solution (solid content concentration 1.68%).

Production Example 2 Preparation of Extract Solution of Apricot Fruit Seeds were removed from the apricot fruit of the genus Rosaceae, and made into a paste with a grinder. After adding 210 g of 1, 3- butylene glycol to 90 g of this fruit paste, it extracted at 4 degreeC. To this, 410 g of purified water was added, followed by filtration to obtain 580 g of a brown clear apricot fruit extract solution (solid content concentration: 1.61%).

Production Example 3 Preparation of apricot flower extract solution The apricot flower of the genus Rosaceae was dried, and 22.5 g of water was added to 1.5 g of the dried flower, and extracted at 80 ° C. This was filtered to obtain 16 g of a brown clear apricot flower extract solution (solid content concentration 2.46%).

Production Example 4 Preparation of the extract solution of the flower of the apricot flower: After drying the flower of the flower of the genus Rosa family cherry, add 15 g of water to 1 g of the dried flower, immerse at 4 ° C., add 15 g of 1,3-butylene glycol Extracted at 4 ° C. The mixture was filtered to obtain 26 g of a brown clear apricot fruit extract solution (solid content concentration: 1.05%).

Production Example 5 Preparation of extract solution of whole grass of hon anz Dry whole grass of von apricot sorghum von apricot genus, add 150 g of water to 10 g of this whole grass dried material, immerse at 4 ° C., and add 1,3-butylene glycol to this And added at 150C and extracted at 4 ° C. This was filtered to obtain 146 g of a brown clear apricot whole grass extract solution (solid content concentration 1.78%).

Prescription example 1. Lotion [A ingredient] part olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butyl paraben 0.1
[B component]
Extract solution of Production Example 1 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide Appropriate amount Purified water Total amount 100 parts [C component]
After heating the components A and B respectively to 80 ° C. or higher, the B component was added to the component A, and the mixture was stirred and homogenized for 2 minutes with HISCOTRON (5000 rpm). After this was cooled to 50 ° C., the C component was added and stirred and mixed, and further cooled to 30 ° C. or less to obtain a lotion.

Prescription example 2. A lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the extract solution of Production Example 2 was used in place of the extract solution of Production Example 1 contained in the component B of the lotion formulation example 1. .

Prescription example 3. A lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the extract solution of Production Example 3 was used in place of the extract solution of Production Example 1 contained in the component B of the lotion formulation example 1. .

Prescription example 4. A lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the extract solution of Production Example 4 was used instead of the extract solution of Production Example 1 contained in the component B of the lotion formulation example 1. .

Prescription example 5. Emulsion [Component A] Part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy Lecithin 1.5
[B component]
Extract solution of Preparation Example 1 3.0
L-Ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
An amount of 100 parts of purified water
[C component]
Appropriate amount of flavor
The above components A and B were each heated to 80 ° C. or higher, and then stirred and mixed. After this was cooled to 50 ° C., component C was added, and the mixture was further stirred and mixed to obtain an emulsion.

Prescription example 6. Emulsion An emulsion was prepared in the same manner as in Preparation Example 5 except that 2.0 parts of tranexamic acid was used instead of 2.0 parts of L-ascorbic acid 2-glucoside and 0.5 parts of potassium hydroxide in Component B of Formulation Example 5. I got

Prescription example 7. Emulsion The emulsion was prepared in the same manner as in Preparation Example 5 except that 3.0 parts of Arbutin was used instead of 2.0 parts of L-ascorbic acid 2-glucoside and 0.5 parts of potassium hydroxide in Component B of Formulation Example 5. Obtained.

Prescription example 8. Emulsion In the same manner as in Formulation Example 5 except that 5.0 parts of Soakuhakuhi extract is used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the B component of Formulation example 5. An emulsion was obtained.

Prescription example 9. Emulsion [Component A] Part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy Lecithin 1.5
[B component]
Extract solution of Production Example 1 5.0
L-Ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Arbutin 3.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
An amount of 100 parts of purified water

Prescription example 10. Essence [component] part ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methyl paraben 0.1
Hyaluronic acid 0.1
Extract solution of Production Example 5 5.0
Citric acid 0.3
Sodium citrate 0.6
Hyaluronic acid was dissolved in purified water so that the total amount of purified water became 100 parts, and then the remaining raw materials were sequentially added and stirred to dissolve to obtain a clear essence.

Example 11. Liquid foundation [A ingredient] part stearic acid 2.4
Propylene glycol monostearate 2.0
Setostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propyl paraben 0.05
[Component B] Part Extract solution of Production Example 1 5.0
Carboxymethylcellulose sodium 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methyl paraben 0.1
Amount that the total amount of purified water is 100 parts [C component]
Titanium oxide 8.0
Talc 4.0
Color Pigment A proper amount of each of the components A and B was mixed and stirred. The mixture was reheated, the above-mentioned component C was added, the mixture was poured into a mold, and the mixture was stirred until room temperature to obtain a liquid foundation.

Prescription example 12. Body Shampoo [Component A] Part N-Lauroylmethylalanine sodium 25.0
Coconut oil fatty acid potassium liquid (40%) 26.0
Coconut oil fatty acid diethanolamide 3.0
Methyl paraben 0.1
[B component]
Extract solution of Production Example 2 5.0
1,3-butylene glycol 2.0
The total amount of purified water is 100 parts. A component and B component are heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued and cooled to room temperature to obtain a body shampoo. The

Example 13. Hair shampoo [A ingredient] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryl dimethylaminoacetic acid betaine 10.0
Coconut oil fatty acid diethanolamide 4.0
Methyl paraben 0.1
[B ingredient] part citric acid 0.1
Extract solution of Production Example 1 5.0
1,3-butylene glycol 2.0
Purified water The amount of component A and B is 100% by weight, respectively. The components A and B are heated to 80 ° C and uniformly dissolved. Then component B is added to component A, stirring is continued, and the mixture is cooled to room temperature to obtain a hair shampoo. The

Example 14. Hair rinse [component A] part polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyl dimethyl ammonium chloride 1.5
Stearyl trimethyl ammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Settanol 3.2
Stearyl alcohol 1.0
Methyl paraben 0.1
[Component B] Part Extract solution of Production Example 1 5.0
1,3-butylene glycol 5.0
Purified water The amount of component A and B is 100% by weight and heated to 80 ° C and dissolved uniformly. Then component B is added to component A, stirring is continued, and the solution is cooled to room temperature to obtain hair rinse. The

Test Example 1 Degranulation Inhibitory Effect Test The anti-inflammatory effect of the extract of apricot (honansu) according to the present invention was evaluated by a cell degranulation inhibitory test. Here, when skin cells are affected by external factors (ultraviolet rays and chemical substances), an antigen is generated, and this antigen causes degranulation of anti-basal spheres and mast cells (histamine, serotonin and eosinophil migration factor etc. Are known to be involved in Since this degranulation is also known to be involved in the development of skin inflammation and allergy, in the present invention, the anti-inflammatory effect was evaluated by an anti-basal degranulation inhibition test.

<Experimental method>
Rat basophil leukemia cells (RBL-2H3) were suspended in Eagle's minimal essential medium containing 10% NCS, seeded at 1 × 10 ^{5 in} 96-well plates and cultured at 37 ° C. for 24 hours. Confluent cells were released with releasing buffer [117 mM NaCl, 5.4 mM KCl, 2.0 mM CaCl, 0.8 mM MgSO4, 5.6 mM
D-glucose, 25 mM HEPES (2- [4- (2-hydroxyethyl) -1-piperazinyl] ethanesulfonic acid), 1 mg / mL
After washing with 200 μL / well of BSA / pH 7.7], a sample solution was prepared by adding the extract solution of Preparation Example 1 to the releasing buffer. This sample solution is added to each well, and 100 μL of 200 μg / mL compound 48/80 (compound 48/80) / releasing buffer is further added to induce degranulation, and incubated at 37 ° C. for 1 hour did. Here, as a sample solution, the thing prepared so that the final concentration (concentration as a solution) of the extract solution of manufacture example 1 to the whole quantity might be 2.0% was used. Further, for comparison, two test sections to which a releasing buffer solution containing a 30% aqueous solution of 1,3-butylene glycol is added instead of the extract solution of Production Example 1 are provided, and one test section (blank) 100 μL each of the releasing buffer solution alone and the other test group (control) were added with 100 μL each of the compound 48/80 / releasing buffer solution for degranulation as described above, and incubated at 37 ° C. for 1 hour. In addition, a green tea extract known as having a degranulation inhibitory effect (an extract prepared to a final concentration of 5% as a solution) was used as a positive control. After induction of degranulation, 50 μL of cell supernatant was aliquoted into another 96-well microplate in order to measure the enzyme activity of β-hexosaminidase released extracellularly. The measurement of β-hexosaminidase activity was performed as follows. That is, 50 mM of each cell supernatant collected on another plate was treated with 5 mM p-nitrophenyl-2-acetamide-2-deoxy-β-glucopyranoside (p-Nitrophenyl-2-acetamide-2-deoxy-β-D-glucocopyranoside) as a substrate. 50 μl) was added and reacted for 30 minutes in a 37 ° C. CO ₂ incubator. The reaction is then stopped by adding 100 μL of 0.2 M glycine buffer (pH 10.7), and the absorbance at 415 nm is measured with an absorbance plate reader (BIO RAD, Model 680) to determine β-hexosaminidase activity. Index of

The results of Test Example 1 are shown in FIG. As shown in FIG. 1, it was revealed that the extract solution according to Production Example 1 of the present invention significantly suppresses the degranulation of anti-basic spheres. In addition, the positive control, green tea extract also showed the same effect, confirming that this test system was performed normally.

Test Example 2 DPPH radical scavenging test <experimental method>
In 20 parts of ethanol was dissolved 2.4 parts of DPPH, to which 20 parts of purified water was added to prepare a DPPH solution. To 24 parts of this DPPH solution, 19.2 parts of an 18 v / v% ethanol solution and 4.8 parts of a 2 M acetic acid-sodium acetate buffer solution (pH 5.5) were added to prepare a DPPH added solution. Also, in order to subtract the influence of the color of the extract itself on the test, a solution obtained by mixing an 18 v / v% ethanol solution and a 2 M acetic acid-sodium acetate buffer solution using 50 v / v% ethanol solution instead of DPPH solution It was a control solution. Next, the extract solution of Production Example 1 was diluted with purified water to prepare a sample solution. Here, as the sample solution, two kinds of concentrations prepared so that the final concentration (concentration as a solution) of the extract solution of Production Example 1 with respect to the total amount is 2.0% and 5.0%, respectively. It was used. The sample solution and DPPH addition solution or control solution are mixed at a ratio of 1: 3 and allowed to stand at room temperature for 10 minutes, and then each test solution is mixed with DPPH addition solution and the absorbance at 550 nm; The difference between the absorbance at 550 nm and the control solution was measured to confirm the residual amount of DPPH radical. At the same time, the same operation as above is performed using a 30% aqueous solution of 1,3-butylene glycol instead of the extract solution of Production Example 1 as a control, and each sample for the DPPH radical residual ratio (100) obtained here The relative value of the DPPH radical retention rate at the time of addition was determined. Further, in order to confirm whether the test system is functioning properly, the same test was conducted also in the case where water-soluble vitamin E (final concentration 25 μM) was added as a positive control instead of the sample solution.

The results of Test Example 2 are shown in FIG. As shown in FIG. 2, it was revealed that the extract solution of Production Example 1 of the present invention exhibited a remarkably superior DPPH radical scavenging effect depending on the concentration. In addition, since the water-soluble vitamin E, which is a positive control, also showed the DPPH radical scavenging effect, it was also confirmed that this test system was performed normally.

Test Example 3 SOD like activity test <experimental method>
50 μL of 0.2 M Tris-HCl buffer solution, 20 μL of 1 mM ethylenediaminetetraacetic acid (EDTA) solution, 10 μL of 0.75 mM nitro blue tetrazolium chloride (NBT) solution, 20 μL of 1 mM xanthine solution, 50 μL of 0.06 U / mL xanthine oxidase solution A sample solution was prepared by mixing 50 μL of the extract solution of Example 1. Here, as a sample solution, the final concentration (concentration as a solution) of the extract solution of Production Example 1 was adjusted to 0.5%, 1.0% and 2.0% with respect to the total amount thereof. Three different concentrations were used. The sample solution was incubated at 37 ° C. for 3 minutes to generate superoxide. Then, the amount of formazan formed by reduction of NBT by superoxide was measured for absorbance at 560 nm. In addition, as a control, the same operation is performed on a solution prepared using a 30% aqueous solution of 1,3-butylene glycol instead of the extract solution of Production Example 1, and the value obtained here is 100, each sample The relative value of the amount of formazan at the time of addition was determined.

The results of Test Example 3 are shown in FIG. As shown in FIG. 3, it was revealed that the extract solution of Production Example 1 of the present invention exhibited SOD-like activity, ie, an active oxygen scavenging action, which was remarkably excellent in a concentration dependent manner.

Test Example 4 Recovery test for rough skin (1)
<Test method> For each of five test subjects (men and women aged 20 to 60), a test area of 1 cm square (test area) was provided on the inner side of each forearm, washed and allowed to rest in a constant temperature and humidity room for 15 minutes. As the initial value, the stratum corneum water content of the test part is measured by the stratum corneum water content measuring device (SKICON-200, manufactured by IBS Co., Ltd.), and the erythema amount is erythema mass measuring device (Mexameter Mexameter (registered trademark) MX18, Courage + Khazaka company) Manufactured by After measurement, 5 w / w% sodium dodecyl sulfate (SDS) aqueous solution was used for occlusion patch for 1 hour in the test part. After completion of the occlusion patch, the test area was washed with water, and after resting for 15 minutes in a room with constant temperature and humidity, the stratum corneum water content and the amount of erythema of the test area immediately after the patch were measured by the above-mentioned measuring device. Then, the lotion (invention sample) of the composition shown in Table 1 which blended the apricot (honanzu) extract of this invention twice a day is applied, and it rests for 15 minutes in a constant temperature and humidity room on the fourth day Then, the stratum corneum water content and the amount of erythema were measured again by the above-mentioned measuring apparatus, and the average value of five subjects was calculated. Moreover, it replaced with the extract solution of manufacture example 1 as control, and performed the same operation as the above using purified water. Furthermore, a control section without SDS treatment is provided, and in this control section, the stratum corneum water content and the amount of erythema are also measured in parallel, and the relative value when the value of the control section is 100, The stratum corneum water content and the amount of erythema were calculated. The stratum corneum water content was calculated as the stratum corneum water content recovery rate according to the following equation 1.
Formula 1: stratum corneum water content recovery rate (%) = (W _4- W ₁ / W _0- W ₁ ) × 100
(W ₀ : initial value of stratum corneum water content, W ₁ : stratum corneum water content immediately after patch, W ₄ : stratum corneum water content after 4 days)

The composition of the sample of the present invention used in Test Example 4 is as shown in Table 1 below.
[Table 1]

The results of Test Example 4 are shown in Table 2.
[Table 2]

The numerical values shown in Table 2 are average values of five subjects. As shown in Table 2, it became clear that the apricot extract of Production Example 1 according to the present invention significantly improves and improves the stratum corneum water content of the skin which was reduced by the influence of SDS. Furthermore, it also became clear that the erythema produced under the influence of SDS was significantly improved. With regard to erythema, it was also confirmed by visual observation that the redness of the skin was significantly recovered.

Test Example 5 Skin primary irritation suppression test (2)
<Test method>
A test section is provided on the inner upper arm of each of five subjects (men and women aged 20 to 60), and after washing, the amount of erythema in the test section of each test subject is used as an initial value erythema quantity measuring device (Mexameter Mexameter (registered trademark) It measured by MX18 and Courage + Khazaka make). Thereafter, an aqueous solution containing Lalores 5 (10% Polyoxyethylene 5 lauryl ether) known as a surfactant and the extract solution of Preparation Example 1 (final concentration 2% as a solution) in the test part was closed patched for 1 hour. After completion of the occlusion patch, the test area is washed with water, and after 3 hours, the amount of erythema of the test area of each subject is measured by the above-mentioned measuring device, and the value obtained by subtracting the value immediately after treatment from the initial value is 5 persons as the primary stimulation suppression amount. The average value of the subjects of was calculated. Moreover, it replaced with the extract solution of manufacture example 1 as control, and performed the same operation as the above using purified water.

The results of Test Example 5 are shown in Table 3.
[Table 3]

The numerical values shown in Table 3 are the average values of five subjects. As shown in Table 3, in the control, the erythema was produced in the test part of the subject under the influence of laureth 5, and the sample of the present invention in which the apricot (hon apricot) extract of Production Example 1 according to the present invention was blended It has been revealed that the effect of preventing and improving erythema is remarkable. In addition, it was also confirmed by visual observation that the redness of the test portion to which the sample of the present invention was applied was significantly suppressed as compared with the test portion to which the control was applied.

As described above, since the extract according to the present invention has remarkably excellent anti-inflammatory and anti-oxidative effects, it has only the effect of preventing and improving skin aging (such as wrinkles and sagging), as well as improving the firmness and gloss. It also has the effect of protecting the skin from oxidative damage and inflammatory damage caused by external factors (ultraviolet rays and chemicals). Therefore, the apricot (honansu) extract according to the present invention is extremely useful as a blending component of anti-aging cosmetics and cosmetics for preventing and improving ultraviolet ray damage. Furthermore, it is also extremely useful as a compounding ingredient for whitening cosmetics that prevents and ameliorates stains, buckwheat, liver spots and the like based on anti-inflammatory and anti-oxidative effects.

  In addition, the extract of apricot (honanzu) according to the present invention has an effect of suppressing primary skin irritation, and further has an effect of preventing or ameliorating inflammation caused by primary skin stimulation, so external factors (ultraviolet rays and It is also extremely useful as a cosmetic ingredient to protect the skin from substances) and irritation substances contained in cosmetics.

Claims (1)

A cosmetic comprising, as an active ingredient, a solvent extract of a mixed solvent of water and a polyhydric alcohol of the fruit of an apricot (Prunus armeniaca Linne var anus Maximowicz) or a hon'anzu (Prunus armeniaca Linne) of the Rosaceae cherry genus (except seeds).