KR102611124B1 - Cosmetic composition for skin exfoliation and skin texture improvement containing gluconic acid - Google Patents

Abstract

It relates to a cosmetic composition for removing dead skin cells and improving skin texture. More specifically, the cosmetic composition contains gluconic acid by treating plum extract or apricot extract with enzymes, thereby providing excellent skin exfoliation effect and skin texture improvement effect. It is characterized by having a.

Description

Cosmetic composition containing gluconic acid for removing dead skin cells and improving skin texture {COSMETIC COMPOSITION FOR SKIN EXFOLIATION AND SKIN TEXTURE IMPROVEMENT CONTAINING GLUCONIC ACID}

The present invention relates to a cosmetic composition for removing dead skin cells and improving skin texture. More specifically, the cosmetic composition contains gluconic acid by treating plum extract or apricot extract with enzymes, thereby providing excellent skin exfoliation effect and skin texture. It is characterized by having a texture-improving effect.

In the skin, the stratum corneum is located in the outermost layer of the skin and plays an important role in protecting the skin from various irritants and pollutants that come in contact with the skin and preventing moisture loss. Generally, keratin naturally exfoliates after a certain period of time and a new keratin layer is formed, but if keratin exfoliation does not occur normally due to internal or external factors such as stress, hormonal abnormalities due to aging, ultraviolet rays, or temperature changes, the skin becomes dull. , Not only does the skin tone become uneven, but it can also cause various skin problems such as acne. To solve this problem, various cosmetics for exfoliation that can artificially remove the stratum corneum have been developed, and can be broadly divided into two types: chemical type and physical type.

First, chemical exfoliating cosmetics are formulations that chemically dissolve and remove dead skin cells using AHA (Alpha Hydroxy Acid), BHA (Beta-Hydroxy Acid), salicylic acid, and plant extracts containing them. Korean Patent Publication No. 10-1325092 discloses a cosmetic composition containing citron extract as an active ingredient to reduce dead skin cells and improve skin texture. These chemical exfoliating cosmetics have the advantage of not needing to be washed off, but they have the disadvantage of being relatively slow in their effectiveness and having very low acidity in most cases, which irritates the skin and causes various skin side effects.

In contrast, the physical exfoliation method involves rubbing cosmetics with various types of scrubs added into the formulation against the skin to remove dead skin cells. There are no chemical side effects on the skin compared to chemical exfoliation formulations, but the particle size is large and rough. When you use a scrub, you can feel a physical stimulation. Commonly used scrubs include synthetic raw materials such as polyethylene, natural scrubs made by grinding hard seeds such as cellulose, mannan, walnut shell, peach pit, or apricot kernel, and crystals such as salt, sugar, calcium chloride, aluminum oxide, and sodium bicarbonate. A scrub agent is being used. Cosmetics are a type of formulation characterized by physically removing dead skin cells using wax, scrub, and gommage. Korean Patent Publication No. 2010-64435 discloses a cosmetic composition for exfoliation that removes dead skin cells and waste by using sodium chloride particles and a surfactant as a scrub agent. These physical exfoliating cosmetic compositions have no chemical skin side effects, but have the disadvantage of physically damaging or irritating the skin.

Accordingly, while conducting research to solve the above problem, the present inventors confirmed that gluconic acid is produced when plum extract or apricot extract is treated with enzymes, and a cosmetic composition containing the gluconic acid was produced. The present invention was completed by discovering that it has excellent efficacy in removing dead skin cells without skin side effects and also has the effect of improving skin texture.

The present invention was made to solve the problems of the prior art described above, and its purpose is to provide a cosmetic composition for removing dead skin cells and improving skin texture.

In addition, the purpose of the present invention is to provide a method for manufacturing the cosmetic composition for removing dead skin cells and improving skin texture.

As a technical means for achieving the above-described technical problem, one aspect of the present invention is,

Provided is a cosmetic composition for exfoliating skin and improving skin texture, comprising an enzyme-treated plum extract or apricot extract, and the enzyme-treated plum extract or apricot extract containing gluconic acid.

The enzyme may include an enzyme selected from the group consisting of glucose oxidase, catalase, and combinations thereof.

The content of gluconic acid may be 5 to 15 parts by weight based on 100 parts by weight of the enzyme-treated plum extract or apricot extract.

The concentration of the total enzyme treated may be 0.01 w/w% to 5 w/w%.

In addition, another aspect of the present invention is,

Mixing dried plums or dried apricots with water and performing hot water extraction; and mixing the hot water extract with an enzyme and subjecting it to primary enzyme treatment, wherein the enzyme-treated hot water extract contains gluconic acid. Preparation of a cosmetic composition for exfoliating skin and improving skin texture. Provides a method.

The weight mixing ratio of the dried plums or dried apricots and water may be 1:1 to 10.

The hot water extraction may be performed at a temperature of 60°C to 100°C for 30 minutes to 2 hours.

The first enzyme treatment may be performed for 3 to 7 hours at a temperature of 30°C to 70°C and a stirring speed of 100 rpm to 300 rpm.

The content of enzyme mixed with 100 parts by weight of the hot water extract may be 0.1 to 2 parts by weight.

The method for producing the cosmetic composition for removing dead skin cells and improving skin texture includes the steps of performing the first enzyme treatment; Thereafter, the step of mixing the primary enzyme-treated hot water extract with an enzyme and subjecting it to secondary enzyme treatment may be further included.

The cosmetic composition for exfoliating skin and improving skin texture according to the present invention as described above may have excellent skin moisturizing, skin barrier, and antioxidant effects by containing gluconic acid.

In addition, the cosmetic composition for removing dead skin cells and improving skin texture may have excellent skin texture improvement and skin exfoliation improvement effects without skin side effects.

Figure 1 is a schematic diagram showing an enzyme reaction mechanism according to an embodiment of the present invention.
Figure 2 is a flowchart showing a method of manufacturing a cosmetic composition for removing dead skin cells and improving skin texture according to an embodiment of the present invention.
Figure 3 is a graph showing the results of HPLC analysis of the enzyme-treated product prepared according to an example of the present invention and the extract prepared according to a comparative example.
Figure 4 is a graph showing the gluconic acid content of the enzyme-treated product prepared according to an example of the present invention.
Figure 5 is a graph showing the moisturizing effect of a cosmetic composition for exfoliating skin and improving skin texture prepared according to an example of the present invention and an extract prepared according to a comparative example.
Figures 6 to 9 are graphs showing the skin barrier effect of a cosmetic composition for exfoliating skin and improving skin texture prepared according to an example of the present invention and an extract prepared according to a comparative example, respectively.
Figures 10 and 11 are graphs showing the antioxidant effect of a cosmetic composition for exfoliating skin and improving skin texture prepared according to an example of the present invention and an extract prepared according to a comparative example, respectively.
Figures 12 and 13 are graphs showing the skin texture improvement effect of a cosmetic composition for exfoliating skin and improving skin texture prepared according to an embodiment of the present invention and an extract prepared according to a comparative example, respectively.
Figures 14 and 15 are graphs showing the skin exfoliation improvement effect of a cosmetic composition for removing dead skin cells and improving skin texture prepared according to an example of the present invention and an extract prepared according to a comparative example, respectively.

Hereinafter, the present invention will be described in more detail. However, the present invention can be implemented in various different forms, and the present invention is not limited to the embodiments described herein, and the present invention is only defined by the claims to be described later.

In addition, the terms used in the present invention are only used to describe specific embodiments and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly dictates otherwise. In the entire specification of the present invention, 'including' a certain element means that other elements may be further included rather than excluding other elements, unless specifically stated to the contrary.

The first aspect of the present application is,

Provided is a cosmetic composition for exfoliating skin and improving skin texture, comprising an enzyme-treated plum extract or apricot extract, and the enzyme-treated plum extract or apricot extract containing gluconic acid.

Hereinafter, a cosmetic composition for exfoliating skin and improving skin texture according to the first aspect of the present invention will be described in detail.

In one embodiment of the present application, the cosmetic composition for exfoliating skin and improving skin texture may include enzyme-treated plum extract or apricot extract. At this time, the enzyme may include an enzyme selected from the group consisting of glucose oxidase, catalase, and combinations thereof. That is, as shown in Figure 1, the plum extract or apricot extract may contain gluconic acid because the enzyme treatment is performed using glucose oxidase, and catalase is used. Because enzyme treatment is performed, hydrogen peroxide (H ₂ O ₂ ) generated during the reaction may be removed.

In one embodiment of the present application, the cosmetic composition for exfoliating skin and improving skin texture may have excellent skin moisturizing, skin barrier and antioxidant effects because it contains gluconic acid, and may provide excellent skin without skin side effects. It may have the effect of improving texture and dead skin cells.

In one embodiment of the present application, the content of gluconic acid may be 5 parts by weight to 15 parts by weight, preferably 8 parts by weight to 12 parts by weight, based on 100 parts by weight of the enzyme-treated plum extract or apricot extract. It may be parts by weight, and according to one embodiment of the present invention, the gluconic acid content of the enzyme-treated plum extract may be about 8.39 parts by weight, and the gluconic acid content of the enzyme-treated apricot extract may be about 11.73 parts by weight. Therefore, as will be described later in the examples and experimental examples below, while ordinary plum extract or apricot extract does not contain gluconic acid, enzyme-treated plum extract or apricot extract contains high gluconic acid as described above, thereby causing skin exfoliation. It may have excellent efficacy for removal purposes.

In one embodiment of the present application, the concentration of the total treated enzyme may be 0.01 w/w% to 5 w/w%, preferably 0.1 w/w% to 1 w/w%, , more preferably, it may be 0.12 w/w% to 0.3 w/w%, and according to an embodiment of the present invention, it may be 0.3 w/w%. At this time, if the concentration of the enzyme is less than 0.01 w/w%, the concentration of the enzyme may be too low and the amount of gluconic acid produced may be reduced, and if it is more than 5 w/w%, the concentration at which gluconic acid can be produced has already been reached. Because it exceeds this, it may be uneconomical.

In one embodiment of the present application, the cosmetic composition for exfoliating skin and improving skin texture may further include a preservative and water. At this time, the content of the enzyme-treated plum extract or apricot extract may be 0.1 to 5 parts by weight based on 100 parts by weight of the cosmetic composition for exfoliating skin and improving skin texture, and according to one embodiment of the present invention, about 2 parts by weight. It may be by weight. If the content of the enzyme-treated plum extract or apricot extract is less than 0.1 part by weight, the skin exfoliation effect and skin texture improvement effect may be reduced, and if it is more than 5 parts by weight, the water content is relatively low, so the cosmetic composition As a result, problems with deterioration of spreadability may occur.

The second aspect of the present application is,

Mixing dried plums or dried apricots with water and performing hot water extraction; and mixing the hot water extract with an enzyme and subjecting it to primary enzyme treatment, wherein the enzyme-treated hot water extract contains gluconic acid. Preparation of a cosmetic composition for exfoliating skin and improving skin texture. Provides a method.

Detailed description of parts overlapping with the first aspect of the present application has been omitted, but the content described in the first aspect of the present application can be applied equally even if the description is omitted in the second aspect.

Hereinafter, the method of manufacturing the cosmetic composition for exfoliating skin and improving skin texture according to the second aspect of the present application will be described step by step in detail with reference to FIG. 2.

First, in one embodiment of the present application, the method for producing a cosmetic composition for removing dead skin cells and improving skin texture may include a step (S10) of mixing dried plums or dried apricots with water (distilled water) and performing hot water extraction. there is.

In one embodiment of the present application, the weight mixing ratio of the dried plums or dried apricots and water may be 1:1 to 10, and preferably about 1:5. On the other hand, the mixture of dried plums or dried apricots and water may be pulverized before hot water extraction, and the pulverization is not particularly limited as long as a normal pulverization method is used. However, according to one embodiment of the present invention, pulverization is performed using a blender. It may be.

In one embodiment of the present application, the hot water extraction may be performed at a temperature of 60°C to 100°C for 30 minutes to 2 hours, and is preferably performed at a temperature of 80°C to 100°C for 30 minutes to 90 minutes. It may be, and according to one embodiment of the present invention, it may be performed at a temperature of 90°C for about 1 hour.

Next, in one embodiment of the present application, the method for producing a cosmetic composition for exfoliating skin and improving skin texture may include mixing the hot water extract with an enzyme and subjecting it to primary enzyme treatment (S20). .

In one embodiment of the present application, the enzyme may include an enzyme selected from the group consisting of glucose oxidase, catalase, and combinations thereof, and by using glucose oxidase Glucose in the hot water extract may be converted to gluconic acid.

In one embodiment of the present application, the content of the enzyme mixed with 100 parts by weight of the hot water extract may be 0.1 part by weight to 2 parts by weight, preferably 0.1 part by weight to 1 part by weight, and According to an example, it may be about 0.66 parts by weight. Meanwhile, the enzyme may preferably be both glucose oxidase and catalase. In this case, the contents of each of the glucose oxidase and catalase are relative to 100 parts by weight of the hot water extract. It may be 0.1 to 0.7 parts by weight, and according to one embodiment of the present invention, the weight mixing ratio of glucose oxidase and catalase may be 3:1. At this time, when the weight mixing ratio of glucose oxidase and catalase is 3:1, the content of gluconic acid in the final produced enzyme-treated hot water extract may be the highest.

In one embodiment of the present application, the material mixed during the first enzyme treatment may further include water (distilled water) in addition to the hot water extract and enzyme, and the content of water is 100 parts by weight compared to 100 parts by weight of the hot water extract. It may be from 300 parts by weight, preferably from 200 to 250 parts by weight, and according to one embodiment of the present invention, it may be about 232 parts by weight.

In one embodiment of the present application, the primary enzyme treatment may be performed at a temperature of 30°C to 70°C and a stirring speed of 100 rpm to 300 rpm for 3 to 7 hours, preferably at 40°C to 60°C. It may be carried out for 4 to 6 hours at a temperature and a stirring speed of 100 rpm to 200 rpm, and according to one embodiment of the present invention, it may be carried out for 5 hours at a temperature of about 50 ° C. and a stirring speed of 150 rpm. You can.

Next, in one embodiment of the present application, the method for producing the cosmetic composition for exfoliating skin and improving skin texture may further include the step of inactivating the primary enzyme-treated hot water extract.

In one embodiment of the present application, the inactivation corresponds to a step of stabilizing the primary enzyme-treated hot water extract before secondary enzyme treatment, and may be performed at a temperature of about 90° C. for 10 minutes.

Next, in one embodiment of the present application, the method for producing a cosmetic composition for exfoliating skin and improving skin texture includes mixing the primary enzyme-treated hot water extract with an enzyme and subjecting it to secondary enzyme treatment (S30); It may include

In one embodiment of the present application, the enzyme may include catalase, and hydrogen peroxide (H ₂ O ₂ ) generated in the first enzyme treatment may be removed through the catalase.

In one embodiment of the present application, the weight mixing ratio of the primary enzyme-treated hot water extract and enzyme may be 100: 0.01 to 5, preferably 0.01 to 1, and according to an embodiment of the present invention, It may be about 99.9:0.1.

Next, in one embodiment of the present application, the method for producing a cosmetic composition for exfoliating skin and improving skin texture may further include the step of inactivating the secondary enzyme-treated hot water extract.

In one embodiment of the present application, the inactivation corresponds to a step of stabilizing the secondary enzyme-treated hot water extract, and may be performed at a temperature of about 90° C. for 10 minutes.

Next, in one embodiment of the present application, the method for producing a cosmetic composition for exfoliating skin and improving skin texture further includes extracting a supernatant by centrifuging the secondary enzyme-treated hot water extract. It may be.

In one embodiment of the present application, the centrifugation may be performed at a speed of 2000 rpm to 6000 rpm for 10 minutes to 30 minutes, and according to an embodiment of the present invention, it is performed at a speed of about 4000 rpm for 20 minutes. It may be possible.

In one embodiment of the present application, the extracted supernatant may be prepared as an enzyme-treated product or PHA in a later step. At this time, the enzyme-treated product may be prepared by filtering the extracted supernatant using a paper filter or a 2 μm filter and then freeze-drying it for 3 days. Additionally, the PHA may be prepared by mixing the extracted supernatant with a preservative and water and then filtering it. In this case, the content of the supernatant may be about 60 wt%.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement it. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein.

Example 1-1. Preparation of a cosmetic composition for exfoliating skin and improving skin texture containing enzyme-treated plum extract (primary enzyme-treated enzyme weight mixing ratio = 3:1)

First, 100 g of dried plums were mixed with 500 g of distilled water (DW) and ground in a blender. Afterwards, the mixture of pulverized dried plums and distilled water was subjected to hot water extraction at a temperature of 90°C for 1 hour.

Next, 30 g of the hot water extract (dried plum: 5 g / distilled water: 25 g) was mixed with 0.15 g of glucose oxidase, 0.05 g of catalase, and 69.7 g of distilled water, and incubated at a temperature of 50°C and 150 rpm. The first enzyme treatment was performed for 5 hours at a stirring speed of .

Afterwards, the primary enzyme-treated product was inactivated at a temperature of 90°C for 10 minutes, and then 0.1 g of catalase was mixed with 99.9 g of the primary enzyme-treated material, at a temperature of 50°C and a stirring speed of 150 rpm. Secondary enzyme treatment was performed for 18 hours.

Next, the secondary enzyme-treated product was inactivated at a temperature of 90°C for 10 minutes, and then centrifuged at a speed of 4,000 rpm for 20 minutes to extract the supernatant.

Thereafter, the supernatants were treated as follows to prepare a cosmetic composition for exfoliating skin and improving skin texture containing enzyme-treated plum extract.

- Plum enzyme-treated product: Obtained by filtering using a paper filter or 2 μm filter and then freeze-drying for 3 days.

- Plum PHA: Mix preservative and water with the supernatant and filter to obtain raw material (supernatant content: 60 wt%)

Example 1-2. Preparation of a cosmetic composition for exfoliating skin and improving skin texture containing enzyme-treated plum extract (primary enzyme-treated enzyme weight mixing ratio = 2:1)

In Example 1-1, a cosmetic composition was prepared using the same method, except that 0.133 g of glucose oxidase and 0.067 g of catalase were mixed during the first enzyme treatment.

Example 1-3. Preparation of a cosmetic composition for exfoliating skin and improving skin texture containing enzyme-treated plum extract (primary enzyme-treated enzyme weight mixing ratio = 1:1)

In Example 1-1, a cosmetic composition was prepared using the same method, except that 0.1 g of glucose oxidase and 0.1 g of catalase were mixed during the first enzyme treatment.

Example 1-4. Preparation of a cosmetic composition for exfoliating skin and improving skin texture containing enzyme-treated plum extract (primary enzyme-treated enzyme weight mixing ratio = 1:2)

In Example 1-1, a cosmetic composition was prepared using the same method, except that 0.067 g of glucose oxidase and 0.133 g of catalase were mixed during the first enzyme treatment.

Examples 1-5. Preparation of a cosmetic composition for exfoliating skin and improving skin texture containing enzyme-treated plum extract (primary enzyme-treated enzyme weight mixing ratio = 1:3)

In Example 1-1, a cosmetic composition was prepared using the same method, except that 0.05 g of glucose oxidase and 0.15 g of catalase were mixed during the first enzyme treatment.

Example 2-1. Preparation of a cosmetic composition for exfoliating skin and improving skin texture containing enzyme-treated apricot extract (primary enzyme-treated enzyme weight mixing ratio = 3:1)

A cosmetic composition for exfoliating skin and improving skin texture containing apricot extract was prepared using the same method as in Example 1-1, except that dried apricots were used instead of dried plums.

Example 2-2. Preparation of a cosmetic composition for exfoliating skin and improving skin texture containing enzyme-treated apricot extract (primary enzyme-treated enzyme weight mixing ratio = 2:1)

In Example 2-1, a cosmetic composition was prepared using the same method, except that 0.133 g of glucose oxidase and 0.067 g of catalase were mixed during the first enzyme treatment.

Example 2-3. Preparation of a cosmetic composition for exfoliating skin and improving skin texture containing enzyme-treated apricot extract (primary enzyme-treated enzyme weight mixing ratio = 1:1)

In Example 2-1, a cosmetic composition was prepared using the same method, except that 0.1 g of glucose oxidase and 0.1 g of catalase were mixed during the first enzyme treatment.

Example 2-4. Preparation of a cosmetic composition for exfoliating skin and improving skin texture containing enzyme-treated apricot extract (primary enzyme-treated enzyme weight mixing ratio = 1:2)

In Example 2-1, a cosmetic composition was prepared using the same method except that 0.067 g of glucose oxidase and 0.133 g of catalase were mixed during the first enzyme treatment.

Example 2-5. Preparation of a cosmetic composition for exfoliating skin and improving skin texture containing enzyme-treated apricot extract (primary enzyme-treated enzyme weight mixing ratio = 1:3)

In Example 2-1, a cosmetic composition was prepared using the same method, except that 0.05 g of glucose oxidase and 0.15 g of catalase were mixed during the first enzyme treatment.

Comparative Example 1. Preparation of common plum extract

In Example 1-1, a common plum extract was prepared by performing only hot water extraction without performing primary or secondary enzyme treatment.

Comparative Example 2. Preparation of general apricot extract

In Example 2-1, a general apricot extract was prepared by performing only hot water extraction without performing primary or secondary enzyme treatment.

Experimental Example 1. Component analysis of enzyme-treated product

HPLC analysis was performed to analyze the components of the plum enzyme-treated product prepared in Example 1-1, the apricot enzyme-treated product prepared in Example 2-1, and the extract according to Comparative Examples 1 and 2, and the results are presented below. It is shown in Table 1 and Figure 3.

[Table 1] - Analysis of gluconic acid components

As shown in Table 1 and Figure 3, in the case of the enzyme-treated product prepared by performing enzyme treatment according to Examples 1-1 and 2-1 of the present invention, the gluconic acid content was 8.39% and 11.73%, respectively, It was confirmed that there was a difference from Comparative Examples 1 and 2 in which gluconic acid was not detected.

In addition, the gluconic acid content of the enzyme-treated products prepared according to Examples 1-1 to 1-5 and 2-1 to 2-5 was measured and shown in FIG. 4.

As shown in Figure 4, it can be seen that the gluconic acid content of the enzyme-treated product was high when the weight mixing ratio of glucose oxidase and catalase was 3:1 to 1:3 during the first enzyme treatment. In particular, it was confirmed that the gluconic acid content of the enzyme-treated products according to Example 1-1 and Example 2-1, which was 3:1, was the highest.

Through this, it was confirmed that the lower the weight mixing ratio of glucose oxidase, the lower the gluconic acid content.

Meanwhile, it was confirmed that there was no significant change in the gluconic acid content when the weight mixing ratio of glucose oxidase and catalase was 3:1 and 2:1, and glucose oxidase was mixed with catalase ( It was expected that there would be no significant increase in the content of gluconic acid even if mixed at a weight exceeding 3 times that of catalase).

Experimental Example 2. Evaluation of the efficacy of PHA

1. Moisturizing efficacy evaluation

In order to evaluate the moisturizing efficacy of the plum enzyme-treated product according to Example 1-1 and the general plum extract according to Comparative Example 1, the expression effect of the moisturizing-related gene (HAS2) was tested through real-time polymerase chain reaction (qRT-PCT). Measurements were made, and the results are shown in Table 2 and Figure 5 below.

[Table 2]

As shown in Table 2 and Figure 5, in the case of the plum enzyme-treated product prepared by performing enzyme treatment according to Example 1-1 of the present invention, compared to the general plum extract according to Comparative Example 1, the moisturizing-related gene (HAS2 ) was confirmed to have an excellent moisturizing effect.

2. Assessment of barrier efficacy

To evaluate the barrier efficacy of the plum enzyme-treated product according to Example 1-1 and the common plum extract according to Comparative Example 1, expression of barrier-related genes (FLG and LOR) was performed through real-time polymerase chain reaction (qRT-PCT). The effect was measured, and the results are shown in Tables 3 and 4 and Figures 6 and 7, respectively (Table 3 and Figure 6 show the effect of FLG expression / Table 4 and Figure 7 show the effect of LOR expression).

[Table 3] - FLG expression effect

[Table 4] - LOR expression effect

As shown in Tables 3 and 4 and Figures 6 and 7, respectively, in the case of the plum enzyme-treated product prepared by performing enzyme treatment according to Example 1-1 of the present invention, compared to the general plum extract according to Comparative Example 1, It was confirmed that it had barrier efficacy due to the excellent expression effect of barrier-related genes (FLG and LOR).

In addition, to evaluate the barrier efficacy of the apricot enzyme-treated product according to Example 2-1 and the general apricot extract according to Comparative Example 2, barrier-related genes (IVL and TGM-) were analyzed through real-time polymerase chain reaction (qRT-PCT). The expression effect of 1) was measured, and the results are shown in Tables 5 and 6 and Figures 8 and 9, respectively (Table 5 and Figure 8 show IVL expression effect / Table 6 and Figure 9 show TGM-1 expression effect).

[Table 5] - IVL expression effect

[Table 6] - TGM-1 expression effect

As shown in Tables 5 and 6 and Figures 8 and 9, respectively, in the case of the apricot enzyme-treated product prepared by performing enzyme treatment according to Example 2-1 of the present invention, compared to the general apricot extract according to Comparative Example 2, It was confirmed that it had barrier efficacy due to the excellent expression effect of barrier-related genes (IVL and TGM-1).

3. Antioxidant efficacy evaluation

To evaluate the antioxidant efficacy of the apricot enzyme-treated product according to Example 2-1 and the regular apricot extract according to Comparative Example 2, DPPH assay and SOD assay tests were performed, and the results are shown in Tables 7 and 8 and Figure 10 below. 11 (Table 7 and Figure 10 show DPPH assay results / Table 8 and Figure 11 show SOD assay results).

[Table 7] - DPPH assay

[Table 8] - SOD assay

As shown in Tables 7 and 8 and Figures 10 and 11, respectively, in the case of the apricot enzyme-treated product prepared by performing enzyme treatment according to Example 2-1 of the present invention, compared to the general apricot extract according to Comparative Example 2, It was confirmed that the antioxidant effect was excellent at high concentrations.

Experimental Example 3. Evaluation of the efficacy of cosmetic compositions for removing dead skin cells and improving skin texture for improving skin texture and dead skin cells

1. Evaluation of skin texture improvement efficacy

The skin texture improvement efficacy of the Sleeping Pack Base formulation containing 2 wt% of plum PHA according to Example 1-1 and the sleeping pack base formulation not containing plum PHA (control product) was evaluated.

Specifically, four healthy adult men and women aged 20 to 60 years old were studied, and the cheek area on both sides of the face was used as the study area.

No adverse skin reactions were observed during the study period, and the results of skin texture improvement efficacy are shown in Table 9 and Figures 12 and 13, respectively.

[Table 9]

(*Improvement rate = (measured value after using the product - measured value before using the product) / measured value before using the product)

As shown in Table 9 and Figures 12 and 13, in the case of the Sleeping Pack Base formulation containing 2 wt% of plum PHA prepared by performing enzyme treatment according to Example 1-1 of the present invention, the plum according to the control product Compared to the sleeping pack base formulation that does not contain PHA, it was confirmed that the skin surface roughness was reduced, and thus the skin texture improvement effect was confirmed to be excellent.

2. Evaluation of skin keratin improvement efficacy

The skin keratin improvement efficacy of the Wash-Off Pack Base formulation containing 2 wt% of plum PHA according to Example 1-1 and the Wash-Off Pack Base formulation not containing plum PHA (control product) were evaluated.

Specifically, five healthy adult men and women aged 20 to 60 years old were studied, and both forearms were studied.

No adverse skin reactions were observed during the study period, and the results of skin keratin improvement efficacy are shown in Table 10 and Figures 14 and 15, respectively.

[Table 10]

(*Improvement rate = (measured value after using the product - measured value before using the product) / measured value before using the product)

As shown in Table 10 and Figures 14 and 15, in the case of the Wash-Off Pack Base formulation containing 2 wt% of plum PHA prepared by performing enzyme treatment according to Example 1-1 of the present invention, the control product Compared to the Wash-Off Pack Base formulation that does not contain plum PHA, it was confirmed that the amount of dead skin cells was reduced, and thus it was confirmed that the effectiveness of improving skin dead skin cells was excellent.

Above, the present invention has been described in detail along with preferred embodiments with reference to the drawings, but the scope of the technical idea of the present invention is not limited to these drawings and examples. Accordingly, various modifications or equivalent embodiments may exist within the scope of the technical idea of the present invention. Therefore, the scope of rights of the technical idea according to the present invention should be interpreted in accordance with the claims, and technical ideas within the scope equivalent or equivalent thereto should be interpreted as falling within the scope of the rights of the present invention.

Claims (10)

Contains enzyme-treated plum extract or apricot extract,
The enzyme-treated plum extract or apricot extract contains gluconic acid,
A cosmetic composition for removing dead skin cells and improving skin texture, wherein the enzyme includes an enzyme selected from the group consisting of glucose oxidase, catalase, and combinations thereof.
delete According to paragraph 1,
A cosmetic composition for exfoliating skin and improving skin texture, wherein the content of gluconic acid is 5 to 15 parts by weight based on 100 parts by weight of the enzyme-treated plum extract or apricot extract.
According to paragraph 1,
A cosmetic composition for removing dead skin cells and improving skin texture, wherein the concentration of the total processed enzymes is 0.01 w/w% to 5 w/w%.
Mixing dried plums or dried apricots with water and performing hot water extraction; and
Mixing the hot water extract with enzyme and subjecting it to primary enzyme treatment;
Including,
The enzyme-treated hot water extract contains gluconic acid,
A method of producing a cosmetic composition for removing dead skin cells and improving skin texture, wherein the enzyme includes an enzyme selected from the group consisting of glucose oxidase, catalase, and combinations thereof.
According to clause 5,
A method for producing a cosmetic composition for removing dead skin cells and improving skin texture, wherein the weight mixing ratio of the dried plums or dried apricots and water is 1:1 to 10.
According to clause 5,
A method of producing a cosmetic composition for removing dead skin cells and improving skin texture, wherein the hot water extraction is performed at a temperature of 60°C to 100°C for 30 minutes to 2 hours.
According to clause 5,
A method of producing a cosmetic composition for removing dead skin cells and improving skin texture, wherein the first enzyme treatment is performed at a temperature of 30°C to 70°C and a stirring speed of 100 rpm to 300 rpm for 3 to 7 hours.
According to clause 5,
A method for producing a cosmetic composition for removing dead skin cells and improving skin texture, wherein the content of the enzyme mixed with 100 parts by weight of the hot water extract is 0.1 to 2 parts by weight.
According to clause 5,
The method for producing the cosmetic composition for removing dead skin cells and improving skin texture is,
The first enzyme treatment step; Since the,
Mixing the first enzyme-treated hot water extract with an enzyme and subjecting it to a second enzyme treatment;
A method for producing a cosmetic composition for removing dead skin cells and improving skin texture, further comprising: