JP3513872B2 - External preparation for skin - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for skin, and more specifically, by blending an acerola extract and an active oxygen scavenger, the production of lipid peroxide caused by active oxygen production in skin and The present invention relates to a skin external preparation such as a cosmetic or an external medicine having an excellent skin aging prevention effect capable of preventing skin inflammation, blackening, aging, etc., and a skin roughness improving effect.

[0002]

2. Description of the Related Art Conventionally, skin external preparations such as emulsions, creams, lotions, packs, detergents, foundations, ointments, and dispersions prevent the production of lipid peroxide and skin inflammation, blackening, and aging. For these purposes, various medicinal components have been added to these. In recent years, as one of the causes of skin aging, the adverse effect of active oxygen on the living body has been cited. Active oxides include superoxide, singlet oxygen,
Hydroxyl radicals, hydrogen peroxide, etc. are known to cause lipid peroxide formation and damage to nuclei, proteins and enzymes, and if a large amount of these is generated, it may cause illness or death. There is. Quantitatively, at least, the biological function is deteriorated due to the accumulation of damage caused by active oxygen, which is a cause of so-called aging phenomenon. Therefore, various active oxygen scavengers have been conventionally developed.

[0003]

Therefore, there is a demand for the development of a highly effective active oxygen scavenger for the purpose of biological defense and prevention of aging. Not only the effect but also the safety must be satisfied. It is an object of the present invention to provide a skin external preparation which is capable of sufficiently exerting the medicinal effect of an active oxygen removing agent, has an excellent effect of removing active oxygen, and is safe and suitable even when applied to a biological system.

[0004]

[Means for Solving the Problems] The present inventors improve the effect of a medicinal component incorporated into an external preparation for the skin for the purpose of preventing the production of lipid peroxide, skin inflammation, blackening and aging. As a result of intensive studies, it was found that by combining the acerola extract and the active oxygen scavenger, the active oxygen scavenging action originally possessed by the active oxygen scavenger is sufficiently exhibited, and the present invention was completed. That is, the present invention is
(A) Acerola extract, and (B) superoxide
Dismutase, Scutellaria extract, Melissa extract and
One or two selected from dl-α-tocopherol acetate
A skin external preparation containing one or more active oxygen scavengers.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION The acerola extract, which is the component (A) of the external preparation for skin of the present invention, is a Malpighia genus.
The fruit tree of Acerola (scientific name Malpighia emar
ginata DC; The scientific name of acerola is differently written in papers and the like depending on the period, region, and researcher. Traditionally Malpig
hia punicifolia L. and Malpighia glabra L. are confused and used as the scientific name of acerola, but nowadays
Edited by Steven Nagy and others in 1980, "TROPI
Malpi described in "CAL AND SUBTROPICAL FRUITS"
ghia emarginata DC is the most suitable. Extraction is carried out from the fruit of 1) using an extraction solvent. The extraction treatment is not particularly limited, but extraction is performed at a low temperature or room temperature or under heating with a suitable solvent.

Examples of the above-mentioned extraction solvent include water; lower monohydric alcohols such as methyl alcohol and ethyl alcohol; liquid polyhydric alcohols such as glycerin, propylene glycol, and 1,3-butylene glycol, and one or more species thereof. Can be used. As an example of a preferred extraction method, purified water is used for extraction at a low temperature, followed by filtration, and 1,3-butylene glycol is further mixed to a concentration of 20 to 70% (W / W). , And a method of performing filtration again.

The compounding amount (content) of the acerola extract in the external preparation for skin of the present invention is preferably 0.00005 to 5% by weight as dry solid content (hereinafter, "% by weight" is simply represented by "%"). And more preferably 0.0005-
1%. If the compounding amount (content) of this acerola extract is less than 0.00005%, a sufficient effect may not be obtained, and even if it exceeds 5%, the effect is further increased. Absent.

Further, the component (B) of the skin external preparation of the present invention
Is superoxide dismutase (SOD),
Ugon extract, Melissa extract and acetic acid dl-α-toco
It is a ferrol . These active oxygen scavengers may be used alone or in combination of two or more.

The compounding amount (content) of the active oxygen removing agent in the external preparation for skin of the present invention is preferably 0.00001.
-10%, more preferably 0.0001-5%.
When the plant extract or the like is used as it is, it is converted to dry solids. And if the active oxygen scavenger is within this range, when combined with the acerola extract, it does not affect the acerola extract in the external preparation for skin, has good stability over time, and has excellent skin aging. A preventive effect and a rough skin improving effect can be obtained. That is, active oxygen removing agent is affected by the acerola extract, generation of lipid peroxides due to active oxygen generated in the skin, skin inflammation, blackening, it increases the effect of preventing aging and the like.

The skin external preparation of the present invention is prepared by blending the essential components (A) and (B) with various forms of bases known as ordinary skin external preparations according to a conventional method. be able to. The form of the external preparation for skin is not particularly limited, and examples thereof include emulsion, cream, lotion, pack,
It can be used as skin care cosmetics such as cleaning agents, makeup cosmetics such as lipsticks and foundations, cosmetics for scalp, external medicines such as ointments and dispersions, etc. , Paste, mousse,
It may be in the form of gel, powder, solution system, solubilization system, emulsion system, powder dispersion system, or multi-layer system.

In addition to the above-mentioned components (A) and (B), the external preparation for skin of the present invention generally contains cosmetics, quasi-drugs, external medicines, etc. within a range not impairing the effects of the present invention. Ingredients used in the formulation, namely water, oils, surfactants, metal soaps, gelling agents, powders, alcohols, water-soluble polymers, film-forming agents, resins, UV protection agents, inclusion compounds, antibacterial agents Agents, fragrances, deodorants, salts, pH adjusters, cooling agents, animal / microorganism derived extracts, plant extracts, blood circulation promoters, astringents, antiseborrheic agents, whitening agents, anti-inflammatory agents, moisturizers, An anti-inflammatory agent, a keratolytic agent, an enzyme, hormones, vitamins and the like can be appropriately added in one kind or two or more kinds.

[0014]

The present invention will be described in more detail with reference to Reference Examples, Test Examples and Examples, but the present invention is not limited thereto.

Reference Example 1. Production of acerola extract Acerola (scientific name: Malpighia emarginata DC)
Purified water was added to the fruit of the above, extraction was performed at low temperature for 1 day, and then filtration was performed to obtain an acerola extract (dry solid content 1.7).
%).

Reference example 2. Manufacture of Scutellaria extract 10 g of Scutellaria baicalensis (root of Scutellaria baicalensis) is shredded, 100 ml of absolute ethanol is added, and 5 times at room temperature with occasional stirring.
It was extracted for a day and filtered to obtain an Agonaceae extract (dry solid content 1.0%).

Test Example 1. Superoxide removing activity measurement test The acerola extract and the active oxygen removing agent shown in Table 1 were used alone or as a mixture to prepare a sample, which was diluted with water and then the superoxide removing activity was measured by the following measuring method.

(Measurement method) 0.05M sodium carbonate buffer (pH 10.2)
The substrate solution [3.0 mM xanthine (0.
Dissolved in 05M sodium carbonate buffer)] 0.1 ml,
3.0 mM EDTA 0.1 ml, 0.15% (w /
0.1 ml of bovine serum albumin of v), 0.1 ml of 0.75 mM nitroblue tetrazolium and 0.1 ml of each test sample were mixed and left at 25 ° C. for 10 minutes. Then, 0.1 ml of an enzyme solution [xanthine oxidase solution (diluted to about 0.04 units / ml with purified water)] was added to start the reaction, and after incubation at 25 ° C. for 20 minutes, 0.1 ml of 6 mM CaCl 2 was added. Stop the reaction. Then, the absorbance (A) at 560 nm is measured. As a control, the absorbance (B) of the sample to which purified water was added instead of the test sample, and 6 mM for the blank of each sample
After the reaction was stopped by adding 0.1 ml of CaCl2, the absorbance (C) of the sample to which 0.1 ml of xanthine oxidase was added was measured, and the superoxide removal rate was calculated from the following formula. The results are shown in Table 1.

[0020]

[Equation 1]

[0021]

[Table 1]

As is clear from the results in Table 1, the acerola extract alone had superoxide removing activity, but when used in combination with the SOD and Scutellariae extract, the acerola extract exhibited a synergistic action and activity. It was shown to be extremely effective in removing oxygen.

Example 1. Cream Cream having the composition shown in Table 2 and the following production method (the present invention product 1,
2 and comparative products 1 to 4) were prepared, and their skin beautifying effect and skin aging preventing effect were examined. The results are also shown in Table 2.

[0024]

[Table 2]

(Production Method) A. Ingredients (1)-(7), (11) and (12) are mixed and heated to 70 ° C. B. Ingredients (8) and (13) are mixed and heated to 70 ° C. C. After adding B to A, mixing, and cooling (9) and (1
0) was added and mixed uniformly to obtain a cream.

(Test Method) For each test cream product, 15 women aged 25 to 55 were used as a panel, and an appropriate amount of the test cream was applied to the face after washing the face twice daily in the morning and at night for 12 weeks. The following criteria evaluated the beautiful skin and the skin aging prevention effect by application.

(Evaluation criteria) Beauty effect: <Evaluation> <Contents> Effective The dullness of the skin is no longer noticeable. Somewhat effective The dullness of the skin became less noticeable. Ineffective No change from before use.

Skin aging prevention effect: <Evaluation> <Contents> Effective The skin's elasticity and gloss were improved. Slightly effective Skin tone and gloss were slightly improved. Ineffective No change from before use.

As shown in the results of Table 2, the product 1 of the present invention (containing the acerola extract and the active oxygen scavenger Melissa extract) and the product 2 of the present invention (the acerola extract and the active oxygen scavenger Melissa extract and acetic acid) A cream of dl-α-tocopherol) is applied to the skin to prevent the occurrence of "dullness" of the skin, to make the skin beautiful, and to improve the suppleness and gloss of the skin and prevent the aging of the skin. It became clear to do.

Example 2 Preparation Example of Cleaning Agent (Prescription) (%) (1) Stearic Acid 10.0 (2) Palmitic Acid 8.0 (3) Myristic Acid 12.0 (4) Lauric Acid 4.0 ( 5) oleyl alcohol 1.5 (6) purified lanolin 1.0 (7) perfume proper amount (8) preservative 0.1 (9) potassium hydroxide 6.0 (10) acerola extract * 1 0.5 (11) ) Scutellaria extract * 2 0.5 (12) Purified water Remaining amount * 1 Manufactured in Reference Example 1 * 2 Manufactured in Reference Example 2

(Production Method) A. Ingredients (9) and (12) are heat mixed and maintained at 70 ° C. B. The components (1) to (6) and (8) are mixed by heating and kept at 70 ° C. C. After adding A to B and keeping at 70 ° C for a while, cooling to 50 ° C after completion of the saponification reaction, components (7), (10) and
(11) was added and cooled to obtain a cleaning agent. The detergent of Example 2 was excellent in stability over time, and when applied to the skin, it was moisturized and excellent in moisturizing property, and the texture of the skin was adjusted to give a firmness and gloss.

[0045]

As described above, by combining the acerola extract and the active oxygen scavenger, the inherent effect of the active oxygen scavenger can be sufficiently exerted.
Therefore, the external preparation for skin of the present invention has extremely high improving and preventing effects on the production of lipid peroxide, inflammation, blackening, aging, etc. due to active oxygen production in the skin. INDUSTRIAL APPLICABILITY The external preparation for skin of the present invention is capable of sufficiently exhibiting the inherent performance of an active oxygen scavenger, and thus is extremely useful in beauty and medicine.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI A61K 35/78 A61K 35/78 N A61P 17/00 A61P 17/00 39/06 39/06 (72) Inventor Shizuka Uehara Tokyo 48-18 Sakaemachi, Kita-ku, Kose Research Headquarters (72) Inventor Ichiro Sasaki Tokyo, Kita-ku 48-18 Sakaemachi, Kose Research Headquarters (72) Inventor Kenichi Nagamine Misumi Higashimurayama, Tokyo Machi 2-5-1-203 (72) Inventor Takashi Kido 1-21-25-103 Namiki, Kawaguchi City, Saitama Prefecture (56) Reference JP-A-2-200610 (JP, A) JP-A-7-277939 ( JP, A) JP 10-114670 (JP, A) JP 9-291011 (JP, A) JP 10-182405 (JP, A) JP 9-183718 (JP, A) (58) ) Fields surveyed (Int.Cl. ⁷ , DB name) A61K ^7/ 00-7/50

Claims (2)

(57) [Claims] 1. (A) Acerola extract, and (B) super.
-Oxide dismutase, Scutellaria extract, Melit
Selected from salmon extract and dl-α-tocopherol acetate
An external preparation for skin comprising one or more active oxygen scavengers. 2. The external skin application according to claim 1, wherein the blended amount of the acerola extract is 0.00005 to 5% by weight as a dry solid content, and the blended amount of the active oxygen scavenger is 0.00001 to 10% by weight. Agent.