JPH09291023A - Preparation for external use for skin - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a preparation for external use for skin, capable of effectively controlling UV-A hinderance and having suppression of pigmentation and/or aging preventing effect and useful as milky lotion, cream, etc. SOLUTION: This preparation for external use for skin contains a substance exhibiting catalase action and a UV-A absorber (e.g. p-methoxycinnamic acid 2-ethylester) and/or a UV-A scattering agent (e.g. zinc oxide). A catalase derived from an animal (e.g. cow), a plant (e.g. spinach) or a microorganism (e.g. a bacterium of the genus Micrococcus) is preferably used as the substance exhibiting catalase action. The content of the substance exhibiting catalase action in the preparation for external use for skin is preferably 10<4> to 10<6> unit/100g product, expressed in terms of catalase.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention is an external preparation for skin which contains a substance exhibiting a catalase action and a UV-A absorber and / or a UV-A scatterer and is effective for protection against UV-A injury. Furthermore, the present invention relates to a skin external preparation which is excellent in pigmentation suppression effect and / or aging prevention effect and effective for UV-A injury protection.

[0002]

2. Description of the Related Art Among the ultraviolet rays, long wavelength ultraviolet rays called UV-A (320 to 400 nm) are shorter than UV-B (280 to 320 nm) which is a shorter wavelength ultraviolet ray.
Since the acute reaction such as inflammation does not occur and the action is mild, the necessity of the protection has not been discussed so far.

A known action of UV-A on the skin has been called immediate blackening, which is a phenomenon of skin blackening that disappears within a few hours immediately after irradiation, or a delayed blackening that lasts for several days. The pigmentation phenomenon is known. Since UV-A reaches the dermis of the skin, it is also reported that it is involved in skin aging. Pigmentation due to such delayed blackening of UV-A and aging at the dermis level have been hitherto dealt with protection using a UV-A absorber and / or a UV-A scatterer. However, external application of a UV-A absorber and / or a UV-A scatterer is not sufficient to prevent the inactivation of catalase, and no other effective protection method has been considered.

On the other hand, catalase is an enzyme that eliminates hydrogen peroxide, exists in every cell in the skin (epidermal cells, fibroblasts, melanocytes, etc.) and protects against active oxygen damage caused by ultraviolet rays. Are known.

Recently, the inactivation of this catalase by UV-A has been reported by an experiment using skin fibroblasts (J. Invest. Dermatol., V.
ol. 100, No. 5P692 to 698 (199
3)). However, the simple topical application of catalase on the skin was not sufficient to protect against UV-A damage.

[0006]

SUMMARY OF THE INVENTION In view of the above situation, the present invention aims to develop a skin external preparation having a pigmentation-suppressing and anti-aging effect by effectively protecting against UV-A injury. It was made.

[0007]

The present inventors have combined a substance having a catalase action with a UV-A absorber and / or a UV-A scatterer to supplement the catalase activity on the skin and cause UV- By blocking the access of A to the skin, U having a pigmentation suppressing and / or antiaging effect
It was found that a skin external preparation effective for VA injury protection can be obtained.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION It is known that a great number of substances exhibit catalase action exist. The origin of the substance showing a catalase action which is an essential component in the present invention is not particularly limited, and may be animal-derived, plant-derived, microbial-derived, or synthetic, for example, the liver of horses, cattle, dogs, mice, etc. , Red blood cells, or animal-derived extracts such as chicken caps, spinach, horseradish,
Extracts and / or squeezed products derived from plants such as soybeans, figs, peanuts, chloroplasts, red wrinkles, yashajitsu, yam sprout, cauliflower, tomato, melon, apple, pear, and banana, Micrococcus lysodeikticu
s), Aspergillus niger
s niger) and the like derived from microorganisms. In the case of natural origin, the extraction method, purification treatment method, etc. may be appropriately selected without any particular limitation.

The content of the substance showing a catalase action in the external preparation for skin of the present invention may be 10 ¹ to 10 ⁷ Units ^per 100 g of the product as a catalase conversion amount, and more preferably 10 ⁴ to 10 ⁶ Units. is there.

Regarding the UV-A absorber and / or the UV-A scattering agent, which is another essential component in the present invention,
There is no particular limitation as long as it is one commonly used in cosmetics and pharmaceuticals, but in the case of a UV-A absorber, for example, para-methoxycinnamic acid 2-ethyl ester, 2-hydroxy-4.
-Methoxybenzophenone, 4-t-butyl-4'-methoxydibenzoylmethane, sodium 2-hydroxy-4-methoxybenzophenone-5-sulfonate, dimethoxybenzylidene dioxoimidazolidine propionate 2-ethylhexyl and the like can be mentioned. Of these, especially 4-t-butyl-4′-methoxydibenzoylmethane,
Sodium 2-hydroxy-4-methoxybenzophenone-5-sulfonate and 2-ethylhexyl dimethoxybenzylidenedioxoimidazolidinepropionate are preferred. Examples of the UV-A scattering agent include zinc oxide, titanium oxide, fine zinc white, fine particle titanium oxide, and the like, and fine zinc white and fine particle titanium oxide are particularly preferable.

The content of the UV-A absorber and / or the UV-A scattering agent in the external preparation for skin of the present invention is 0.01-10.
% (Hereinafter, simply indicated as "%"), and more preferably 0.05 to 5%.

The external preparation for skin of the present invention is produced by combining a substance exhibiting a catalase action and a UV-A absorber and / or a UV-A scattering agent with a known base for external preparation for skin according to a conventional method. It

The form of the external preparation for skin is not particularly limited, and may be cosmetics such as milky lotion, cream, foundation, lotion, body lotion, lipstick, and pharmaceuticals such as ointment, external preparation, cream and the like. As a base for external preparations, a base depending on the form of these external preparations,
For example, purified water, lower alcohols, polyhydric alcohols, oils and fats, surfactants, powders, cosmetic ingredients, UV-B absorbers, U
VB scattering agents, thickeners, pigments, preservatives, perfumes and the like can be used.

[0014]

EXAMPLES The present invention will be described in more detail with reference to Production Examples, Test Examples and Examples, but the present invention is not limited thereto.

Production Example 1 Production of Melon Extract To 50 g of melon pulp, 150 ml of purified water was added, homogenized and filtered to obtain a melon extract.

Production Example 2 Production of Chicken Cob Extract 50 g of chicken cob was washed with water and then chopped, and purified water containing 0.01% proteolytic enzyme (Actinase E, 1 × 10 ⁶ PU / g, manufactured by Kaken Pharmaceutical Co., Ltd.) (150 ml) was added, and the mixture was heated at 60 ° C. for 8 hours and then filtered to obtain a chicken cob extract.

Production Example 3 Production of Yashajitsu Extract 10 g of yashajitsu fruit was finely chopped, 100 ml of 50% ethyl alcohol was added, and the mixture was extracted at room temperature for 3 days with occasional stirring and then filtered to obtain a yashajitsu extract.

Test Example Catalase activity measurement The catalase activity of each extract produced in Production Examples 1, 2 and 3 was measured by the following method. That is, 0.05
Production examples 1 and 2 using M phosphate buffer (pH 7.0)
And n (2 × 10 ⁴ to 2 ×
It was diluted 10 ⁷ ) times to obtain a test solution. Furthermore, 0.1 ml of 30% hydrogen peroxide solution is added to 0.05M phosphate buffer (pH
7.0) A solution added to 50 ml was used as a substrate, and 0.1 ml of a test solution was added to 2.9 ml of this substrate solution. Then
At 20 ° C., the change in absorbance at 240 nm is measured and the absorbance drops from 0.450 to 0.400, ie
The time (T minute) at which hydrogen peroxide decomposed by 3.45 μmol was measured, and the catalase activity was calculated according to the following formula (1). The measurement results are shown in Table 1. The catalase activity indicates the number of μmol of hydrogen peroxide decomposed in 1 minute.

[0019]

[Equation 1]

[0020]

[Table 1]

As is clear from Table 1, the melon extract,
It was shown that the chicken cob extract and the yashajitsu extract had high catalase-like activity.

Examples 1 to 3 and Comparative Examples 1 to 4 Foundation A foundation was prepared by the composition shown in Table 2 and the following production method, and its pigmentation inhibiting effect was examined. Table 3 shows the results.

[0023]

[Table 2]

(Production Method) A. Mix components (1)-(7). B. The components (8) to (11) are mixed by heating and kept at 75 ° C. C. Add B and components (12) and (13) to A, mix and grind to homogenize. D. Press C to obtain foundation.

(Test method) 28 males aged 25 to 45 years were used as a panel, and the pigmentation suppressing effect was measured for 7 foundation products of Examples 1 to 3 and Comparative Examples 1 to 4.
That is, after applying 2 mg / cm ² of each sample on the back (3 items per person, 12 items per item), UV-A was applied to 4 parts.
The degree of blackening one week after irradiation with 0 J / cm ² was evaluated according to the following criteria. The results are shown in Table 3.

(Evaluation Criteria) Evaluation state Effective blackening is not recognized Slightly effective Blackening is recognized Ineffective Strong blackening is observed

[0027]

[Table 3]

As shown in the results of Table 3, the product of the present invention is UV
It became clear that pigmentation by -A irradiation was effectively suppressed.

Examples 4 to 6 and Comparative Examples 5 to 8 Emulsion An emulsion was prepared with the composition shown in Table 4 and the following production method, and its photoaging resistance effect was examined. The results are shown in Table 5.

[0030]

[Table 4]

(Production method) A. Ingredient (6) is dispersed in a portion of ingredient (14). B. The remaining amounts of the components (7) to (10), (13) and (14) are mixed by heating and kept at 70 ° C. C. The components (1) to (5) and the component (11) are mixed by heating and kept at 70 ° C. D. After adding B to C and mixing uniformly, the components (12) and A are added to uniformly emulsify, and the mixture is cooled to 30 ° C. to obtain an emulsion.

(Test method) U was applied to 6-week-old hairless mice.
The effect on the photo-aging model mouse produced by irradiating VA was measured. That is, 42 J / cm ² of UV-A was irradiated 5 times a week for 20 consecutive weeks, and 0.1 ml / mouse was applied to the back of the mouse as a sample. Six animals were used in each group, and scored for elastin deposition, which is a characteristic phenomenon of wrinkle formation and photoaging, according to the following criteria. Wrinkle formation was observed by observing the appearance of the skin, and elastin deposition was evaluated by observing the tissue sections of the dorsal skin and then staining them with Fontana Masson by light microscopy. 1 for each score
The scores of 6 animals in the group were averaged to obtain the result. Table 5 shows the results.

(Evaluation Criteria) <Wrinkle formation score> Score State 0 No wrinkles 1 Fine wrinkles 2 Coarse wrinkles <Elastin deposition score> Score state 0 No elastin deposition 1 Slight elastin deposition 2 Elastin deposition

[0034]

[Table 5]

As shown in the results of Table 5, the product of the present invention suppresses wrinkle formation and elastin deposition, and thus UV-
It was revealed that the photoaging due to A was effectively suppressed.

Creams of Examples 7 to 9 and Comparative Examples 9 to 12 Creams having the compositions shown in Table 6 and the following production method were prepared, and the skin beautifying effect was examined. Table 7 shows the results.

[0037]

[Table 6]

(Production Method) A. The component (8) is dispersed in a part of the component (13). B. The components (1) to (7) and the components (10) to (12) are homogenized by heating and mixed, and kept at 70 ° C. C. The remaining amounts of the component (9) and the component (13) are heated and mixed,
Keep at 70 ° C. D. After adding B to C and emulsifying, add A and mix,
Cool to ℃ to obtain a cream.

(Test method) 27 per cream of test
~ 15 women aged 54 years old will be on the panel, and twice a day every morning 12
The test cream was applied in an appropriate amount for a week, and the effect of improving the skin tone of the present invention was evaluated by evaluating the effect of improving dullness and texture.

(Evaluation Criteria) <Dullness evaluation> Evaluation state Effective Dullness is less noticeable Effective Dullness is less noticeable Invalid Before use and no change <Texture evaluation> Evaluation state Effective Good texture and slightly effective Texture is slight Aligned to invalid before use and unchanged

[0041]

[Table 7]

As shown in the results in Table 7, the product of the present invention has the effect of making dullness inconspicuous and adjusting the texture by continuous use.

Example 10 Ointment: (Component) (%) (1) Stearic acid 18.0 (2) Cetanol 4.0 (3) Triethanolamine 2.0 (4) Glycerin 5.0 (5) Melon extraction Substance ^{* 1} 1.0 (6) Dimethoxybenzylidene dioxoimidazolidine 2-ethylhexyl propionate ^{* 2} 0.1 (7) Purified water Remaining amount

* 1 Manufactured in Manufacturing Example 1 * 2 Manufactured by Ajinomoto Co., Inc.

(Production Method) A. A part of the components (3), (4), (6) and (7) is mixed by heating and kept at 75 ° C. B. Heat mix components (1) and (2) and maintain at 75 ° C. C. Add A slowly to B. D. While cooling C, (5) dissolved in the rest of (7) was added to obtain an ointment.

The ointment of Example 10 suppressed the formation of wrinkles and the deposition of elastin, and effectively suppressed the photoaging due to UV-A.

Example 11 Pack: (Component) (%) (1) Polyvinyl alcohol 20.0 (2) Ethyl alcohol 20.0 (3) Glycerin 5.0 (4) Kaolin 6.0 (5) Chicken comb extract ^{* 1} 1.0 (6) Fine zinc white ^{* 2} 3.0 (7) Preservative proper amount (8) Perfume proper amount (9) Purified water remaining amount

* 1 manufactured in Production Example 2 * 2 manufactured by Sakai Chemical Co., Ltd.

(Production method) Mixing components (1), (3), (4) and (9),
Heat to 70 ° C. and stir. B. Mix components (2), (7) and (8). C. The above B was added to the above A, mixed, cooled, and (5) and (6) were uniformly dispersed to obtain a pack.

The pack of Example 11 effectively suppressed pigmentation due to UV-A irradiation and gave a transparent skin.

Example 12 Lotion: (Component) (%) (1) Glycerin 2.0 (2) 1,3-butylene glycol 2.5 (3) Polyoxyethylene (20EO) sorbitan monolaurate 1.2 (4) Ethyl alcohol 8.0 (5) Yashajitsu extract ^{* 1} 1.0 (6) 2-Hydroxy-4-methoxybenzophenone-5-sulphonate sodium ^{* 2} 0.5 (7) Preservative Suitable amount (8) Perfume proper amount (9) Purified water remaining amount

* 1 Manufactured in Manufacturing Example 3 * 2 Manufactured by Shonan Chemical Industry Co., Ltd.

(Production Method) A. Components (3), (4), (7) and (8) are mixed and dissolved. B. Components (1), (2), (5), (6) and (9) are mixed and dissolved. C. A and B were mixed and made uniform to obtain a lotion.

The lotion of Example 13 effectively suppressed the formation of wrinkles due to UV-A irradiation, and made the skin smooth and beautiful.

[0055]

INDUSTRIAL APPLICABILITY The external preparation for skin of the present invention contains a substance showing a catalase action and a UV-A absorber and / or a UV-A scatterer to effectively prevent damage by UV-A. It is excellent in pigmentation suppressing effect and / or aging preventing effect.

Continuation of front page (51) Int.Cl. ⁶ Identification number Office reference number FI Technical display location A61K 7/02 A61K 7/02 Z 7/42 7/42 35/12 AED 35/12 AED 35/18 35 / 18 35/407 ADA 35/407 ADA 35/70 35/70 35/74 35/74 D 35/78 35/78 C J 38/44 ADS C09K 3/00 104 C09K 3/00 104 A61K 37/50 ADS

Claims (9)

[Claims] 1. An external preparation for skin comprising a substance exhibiting a catalase action and a UV-A absorber and / or a UV-A scatterer. 2. The external preparation for skin according to claim 1, wherein the substance exhibiting a catalase action is catalase. 3. The external preparation for skin according to claim 1, wherein the substance having a catalase action is an animal-derived substance. 4. The animal-derived substance is one or more selected from bovine, equine, canine, and murine liver extracts, bovine, equine, canine, and murine red blood cell extracts, and chicken cob extracts. The external preparation for skin according to claim 3. 5. The external preparation for skin according to claim 1, wherein the substance exhibiting a catalase action is a plant-derived substance. 6. The plant-derived substance is selected from spinach, horseradish, soybean, fig, peanut, chloroplast, red sprout, yashajitsu, yakkouto, kaidouhi, tomato, melon, apple, pear, banana extract and / or pressed product. The external preparation for skin according to claim 5, wherein the external preparation is one kind or two or more kinds. 7. The external preparation for skin according to claim 1, wherein the substance exhibiting a catalase action is a substance derived from a microorganism. 8. The microorganism-derived substance is Micrococcus lysod.
eikticus), Aspergillus niger (Asp
The external preparation for skin according to claim 7, which is one kind or two or more kinds selected from the extract of Ergillus niger). 9. A UV-A injury defense having a pigmentation-inhibiting effect and / or an aging-preventing effect, which comprises a substance exhibiting a catalase action and a UV-A absorber and / or a UV-A scattering agent. External preparation for skin.