JP2001114634A - Catalase protecting agent and antiager cosmetic including the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a catalase protecting agent capable of inhibiting aging of skin due to erasing hydrogen peroxide imparting a large effect on aging of skin. SOLUTION: The extract of one or more plants such as Asteraceae, Moraceae, ucurbitaceae, Labiatae, Gnamineae, Rosaceae, Rutaceae, Caprifoliaceae, Equisetaceae, Typhaceae, Boraginaceae, Saururaceae and Umbelliferae is a catalase protecting agent excellent in erasing activity to hydrogen peroxide. The antiager cosmetic containing the extract is excellent in prevention/improvement of skin aging concerning with hydrogen peroxide.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a catalase protective agent and an anti-aging composition containing the same, and more particularly, to a method for eliminating hydrogen peroxide which greatly affects skin aging to prevent skin aging. The present invention relates to a catalase protecting agent that can be used and a composition containing the same, such as a cosmetic for preventing aging.

[0002]

2. Description of the Related Art Catalase is an enzyme that decomposes into water and oxygen, and has an action of decomposing hydrogen peroxide caused by aging by hydroxy radicals and the action of SOD. It is estimated that it plays a major role in preventing aging. However, catalase is easily deactivated by irradiation with light such as ultraviolet rays, and therefore, in the case of so-called photoaging involving light, there are many cases where its function is inhibited,
For this reason, light often promotes aging. Furthermore, under light irradiation, there is an environment in which production of hydrogen peroxide is promoted due to inflammation and the like, and such inactivation of catalase has been a serious problem in considering skin aging.

[0003] In addition, hydrogen peroxide is not only a long-lived active oxygen species itself, but also a precursor of a hydroxyl radical, which is said to be the most reactive and harmful of radical species. Hydroxy radicals are known to denature, decompose and polymerize collagen, elastin, hyaluronic acid, etc., which are dermal matrix components.Hydroxy radicals accumulate in the skin with long-lived hydrogen peroxide. It is conceivable that they attack skin constituents via the skin and promote skin aging.
In addition, it has been confirmed that hydrogen peroxide not only seriously damages dermal fibroblasts that produce skin constituents, but also has a strong effect of decomposing DNA. In addition, late Maillard reaction products (AGEs), which are known to be deposited on the dermal matrix component of the wrinkle-forming portion,
According to in vitro experiments, it is also reported that hydrogen peroxide promotes its production. In this sense, the role of catalase in preventing aging is not small.

[0004] The accumulation of hydrogen peroxide harmful to the skin upon irradiation with ultraviolet light or with aging is considered to be one of the major factors of skin photoaging, such as one cause of wrinkles. Therefore, eliminating hydrogen peroxide is considered to be very important in preventing skin aging. However, most of the conventional anti-aging agents focused on lipid peroxidation suppression, superoxide, hydroxyl radical or antioxidant, and very few focused on elimination of hydrogen peroxide. In other words, although the importance of anti-aging and the importance of catalase are recognized, it can be said that there has been no idea connecting them.

On the other hand, in the field of cosmetics and the like, aging that causes wrinkles and sedimentation is an important problem, and prevention of this has been a major problem for a long time. This was also true for functional foods such as health foods.

[0006]

Therefore, in order to develop a more effective anti-aging agent, we have developed an anti-aging agent which protects catalase which scavenges hydrogen peroxide. Accordingly, an object of the present invention is to provide a catalase protective agent which is excellent in preventing and improving the aging of the skin and which is highly safe, and which contains an anti-aging cosmetic for preventing and improving the aging of the skin, An object of the present invention is to provide a composition for preventing aging such as food for preventing aging.

[0007]

Means for Solving the Problems The present inventors have made intensive research efforts to solve the above-mentioned problems, and as a result, in order to prevent light-related aging, it is necessary to prevent catalase inactivation by irradiation with light. Is important. Based on this knowledge,
As a result of eagerly searching for a substance having an anti-aging effect that protects catalase that eliminates hydrogen peroxide from light irradiation, Asteraceae,
Mulberry, Cucurbitaceae, Labiatae, Gramineae, Rosaceae, Rutaceae,
Plants of the family Honeysuckle, Rhododendronaceae, Grassicaceae, Echinaceae, Aceraceae or Apiaceae, more specifically Anthemis
Genus ia, Morus, Luffa, Lavandu
genus la, Artemisia, Helianthus
Genus, Panicum genus, Isodon genus, Prunus
Genus, Saponaria, Achillea, Ci
genus trus, genus Lonicera, Equisetum
Genus, Perilla, Typha, Houttuy
genus Mia, Thymus, Stevia, Lith
The present invention has been completed by finding that the essence of a plant belonging to the genus osperum or Panax, particularly preferably an extract obtained using a specific solvent or a solvent-removed product thereof has a strong catalase protecting action.

That is, the present invention relates to a plant of the family Asteraceae, Mulaceae, Cucurbitaceae, Labiatae, Gramineae, Rosaceae, Citrus family, Honeysuckle family, Rhododendronaceae, Gamaceae family, Purple family, Dokudami family or Apiaceae family, More preferably, genus Anthemisia,
Genus Morus, genus Luffa, genus Lavandula,
Artemisia, Helianthus, Pa
genus nicum, Isodon, Prunus, Sa
genus ponaria, Achillea, Citrus
Genus, Lonicera, Equisetum, Pe
genus rilla, genus Typha, Houttuymia
Genus, Thymus, Stevia, Lithosp
the essence of plants of the genus erum, Panax, preferably these plants as water, alcohols, polyols, esters, nitriles, ethers, ketones,
One or two selected from halogenated hydrocarbons
A catalase protective agent containing an extract obtained by extraction with at least one kind of solvent and / or a solvent-removed product thereof as an active ingredient, and a composition such as an antiaging cosmetic or food containing the same. It is.

[0009]

DESCRIPTION OF THE PREFERRED EMBODIMENTS (1) Catalase Protecting Agent of the Present Invention The catalase protecting agent of the present invention is an essence of the above-mentioned plant. Here, the essence is a part or all of the plant itself, a processed product obtained by drying, crushing, shredding, etc.,
A solvent is added to a plant or a processed product, and the extract or the solvent-extracted product of the extract is further collectively referred to as a purified product obtained by fractionating and purifying the same, and is preferable as the catalase protecting agent of the present invention. Can be extracted from the plant using one or more solvents selected from water, alcohols, polyols, esters, nitriles, ethers, ketones, and halogenated hydrocarbons. And, if necessary, removing the solvent.

The extract has a function of protecting the activity of catalase and contains a component for preventing aging of the skin, and the catalase protecting agent of the present invention only needs to contain such a component. Therefore, the catalase protective agent of the present invention may be one obtained by cutting, crushing, or pulverizing a plant, and preferably contains an extract obtained by extracting the above component with a solvent as an active ingredient. The component can be obtained without any limitation if it is part or all of a specific plant, and examples thereof include roots, stems, leaves, flowers, fruits, and seeds. These may be used alone, or two or more parts may be used. The most preferred of these is the leaf. Examples of the solvent used for extraction include water, alcohols such as methanol and ethanol, 1,3-butanediol, propylene glycol and glycerin, esters such as ethyl acetate and methyl formate, nitriles such as acetonitrile, diethyl ether and the like. Examples thereof include ethers such as tetrahydrofuran, halogenated hydrocarbons such as chloroform and methylene chloride, and ketones such as acetone and methyl ethyl ketone. One or more of these may be used alone or as a mixture. Most preferred of these are alcohols. The extraction may be carried out, for example, by adding a solvent in an amount of 2 to 10 times the amount of the dried plant material or its pulverized material, and immersing it for several days at room temperature or for several hours at a temperature near the boiling point. Then remove unnecessary matter by filtration, etc.
It may be concentrated under reduced pressure. In addition, this is silica gel, OD
It may be purified by column chromatography using a column filled with S, an ion exchange resin or the like.

(2) Anti-aging composition containing the catalase protecting agent of the present invention The anti-aging composition of the present invention is characterized by containing the catalase protecting agent described above. As the composition of the present invention, as long as the catalase of the present invention can deliver the protective agent into a living body, it can be applied without any particular limitation, and as a preferred form, it is particularly preferably applied to food or cosmetics. Can be

The cosmetics to which the present invention is applied have no particular limitation on the dosage form and application. For example, the dosage form may be any of a solution, an emulsion, a cream, an aqueous gel, and the like. In addition to the base material, there may be mentioned finishing materials such as foundations and control colors. These cosmetics can be manufactured by the same method as ordinary cosmetics except that the above-mentioned catalase protective agent is blended.

The content of the catalase protecting agent is not particularly limited, but is preferably 0.01 to 10% by weight, more preferably from the viewpoint of lowering the transdermal absorption efficiency at an effective concentration and a high concentration. More preferably, it is contained in cosmetics in the range of 0.05 to 5% by weight. In addition, the cosmetics of the present invention include various components generally used in cosmetics,
That is, hydrocarbons such as petrolatum and microcrystalline wax, esters such as jojoba oil and gay wax,
Triglycerides such as beef tallow and olive oil, higher alcohols such as cetanol and oleyl alcohol, fatty acids such as stearic acid and oleic acid, glycerin and 1,3-
Polyhydric alcohols such as butanediol, nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, thickeners such as ethanol and carbopol, preservatives, ultraviolet absorbers, antioxidants , A pigment, a powder, a fragrance, an antioxidant, a pH adjuster, a chelating agent, a preservative, or an ultraviolet ray protective agent, an anti-inflammatory agent, a whitening agent and the like can be blended as optional components.

The food is also different depending on the type of essence, but preferably 0.01 to 10% by weight, more preferably 0.05 to 5% by weight from the viewpoint of lowering the absorption efficiency at an effective concentration and a high concentration. % Is preferable. The food can be applied to ordinary foods, but is preferably applied to health foods and the like.
Such foods can also contain any components, such as excipients, binders, coatings, lubricants, sugar coatings, disintegrants, bulking agents, and flavoring. Agent,
Preferred examples include emulsifying / solubilizing / dispersing agents, stabilizers, pH adjusters, and isotonic agents.

The composition of the present invention has a preventive effect on aging,
In particular, it is excellent in preventing aging caused by light. It is suitable to be used for such a purpose.

[0016]

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited to these Examples. The percentages used below are by weight unless otherwise specified.

Example 1 Chamomile (Anthemis)
To 3 kg of fresh leaves of the genus ia) was added 5 l of an 80% aqueous ethanol solution, and the mixture was heated under reflux for 2 hours, and unnecessary substances were removed by filtration, followed by concentration under reduced pressure to obtain 49 g of the catalase protective agent 1 of the present invention. In a similar manner, catalase protectant 2 from mulberry (genus Morus), catalase protectant 3 from loofah (genus Luffa), lavender (genus Lavandula)
Catalase protective agent 4, Artemisia (Artemi)
catalase protectant 5 from the genus sia, sunflower (He
lianthus), catalase protectant 7 from dog millet (Panicum),
Catalase protectant 8 from Hioki sorghum (Genus Isodon), catalase protectant 9 from peach (Genus Prunus), Catalase protectant 10 from Saponaria (Genus Saponaria), Yarrow (Genus Achillea)
Catalase Protectant 11, Orange (Citrus)
Genus), catalase protective agent 12, honeysuckle (Lon
catalase protectant 13 from genus Icera, catalase protectant 1 from horsetail (Equisetum)
4. Catalase protectant 15 from Achillea millefolium (Achillea), Daidai (Citrus)
Catalase protecting agent 16, perilla (Perilla)
Genus), lemon (Citru)
s genus), catalase protecting agent 18, Kogama (Typh
a), catalase protective agent 19,
a catalase protectant 20 from the genus tuymia, a catalase protectant 21 from the genus Thymus, a catalase protectant 22 from the stevia genus, Lithospermum
Genus), catalase protecting agent 23, carrot (Pana)
x) was obtained.

Example 2 49 g of catalase protective agent 1
Is dispersed and dissolved in 1 l of water, charged into a column filled with Diaion HP-20, washed by flowing 2 l of water, and washed with 5 l of water.
Elution was carried out by flowing 5 l of a 0% aqueous ethanol solution. This was concentrated to obtain 22 g of catalase protective agent 25.

Example 3 3 kg of dried whole carrot (genus Panax) was cut into small pieces, added with 10 l of 50% ethanol, refluxed for 2 hours, filtered and concentrated under reduced pressure to obtain 76 g of the catalase protecting agent of the present invention. 26 was obtained.

Example 4 Measurement of Anti-Aging Activity (Measurement of Catalase Activity In Vitro) Hydrogen peroxide is disproportionated by catalase to generate water and oxygen. Since the amount of generated oxygen is proportional to the amount of catalase, the catalase protection effect was measured using this principle. That is, a hydrogen peroxide solution adjusted to 0.02M.
2 ml was taken in the cell of the oxygen electrode meter, and when the temperature of the solution in the cell heated by a water bath reached 30 ° C., 50 μl of a solution containing 10 units of catalase derived from bovine liver and containing 50 μg of the catalase protective agent of the present invention was placed in the cell. Inject,
The amount of oxygen generated per minute was measured. As a standard, use only catalase solution containing no catalase protective agent.
A test plot injected with 0 μl was prepared. For both the catalase protective agent test section and the standard test section, the same operation was performed for a solution irradiated with a long-wavelength ultraviolet ray (BLB lamp; manufactured by Toshiba Corporation) for 1 hour.

The results are shown in Table 1. The amount of oxygen generated in the non-irradiated catalase protective agent test group assuming the amount of oxygen generated in the non-irradiated standard test group to be 100% was determined as the residual ratio of the added activity. The oxygen generation amount in the test plot was determined as the irradiation activity residual rate. Next, the product of the residual ratio of the added activity and the residual ratio of the irradiation activity was determined as the total residual ratio, and the catalase protective action was evaluated. In the control example where only catalase was irradiated with light,
Catalase was reduced to 22.5%.

[0022]

[Table 1]

From these results, it can be seen that the extract of the specific plant, which is the catalase protecting agent of the present invention, has an excellent catalase protecting effect from light. This indicates that the catalase protecting agent of the present invention can prevent catalase from being degraded by light and preventing prevention of aging.

Example 5 Using a group of five hairless mice, the effect of inhibiting photoaging in a photoaging model using ultraviolet light was examined. That is, the hairless mouse was treated by applying 0.1 ml of a 50% aqueous ethanol solution containing 0.1% of the extract powder of the catalase protective agent 1,5,7,8 of Example 1, and then treating it with 1 / MED. Irradiation with 3 times the amount of ultraviolet light (BLB lamp; manufactured by Toshiba Corporation) was performed. This work 5 times / 1
Performed at a weekly rate for 8 weeks, and compared the skin elasticity state with the average level of the control group without UV irradiation and sample administration, ++: very elastic, +: elastic,
±: Slight loss of elasticity,-: Standard of severe loss of elasticity, and also the dullness of skin color. ++: Almost no dullness, +: Dullness is suppressed, ±: Slight dullness Suppressed,-: Evaluated based on the criteria that dullness was not suppressed. In addition, a group to which a 50% aqueous ethanol solution containing 0.1% of powder obtained by treating ascorbic acid or tea leaves in the same manner as the catalase protective agent 1 was provided as a comparative control. Table 2 shows the results. This indicates that the extract of the specific plant, which is a catalase protective agent, has an effect of preventing aging caused by light in a photoaging model.

[0025]

[Table 2]

Example 6 A lotion was prepared according to the following formulation. That is, the ingredients were stirred and solubilized at room temperature to obtain a lotion. Using this lotion twice daily in the morning and evening for three months, using a group of three panelists suffering from dullness, often exposed to ultraviolet rays during work outside, to prevent and improve the effect of dullness I was evaluated. The evaluation criteria are: rating 2: marked prevention / improvement, rating 1: obvious prevention / improvement, rating 0.5: slight prevention / improvement, rating 0: no improvement. As a control, the extract powder of catalase protective agent 8 was replaced with water, and as a comparison,
The tea extract was replaced with a tea extract powder extracted in the same manner as the catalase protective agent 8. Table 3 shows the results. From this, the lotion containing the catalase protective agent of the present invention,
It was found to be effective in preventing and improving aging such as dullness. Extract powder of catalase protective agent 8 0.1 part by weight 1,3-butanediol 5 parts by weight Glycerin 3 parts by weight Sodium citrate 0.1 part by weight Methylparaben 0.2 parts by weight Ethanol 8 parts by weight Water 83.7 parts by weight

[0027]

[Table 3]

Example 7 A cream was prepared according to the following recipe. That is, a, b, and c were each heated and dissolved at 80 ° C., and b was gradually added to b, and after further adding c to emulsify, the homogenized particles were homogenized by a homomixer and cooled to obtain a cream. .

A) Squalane 10 parts by weight Cetanol 3 parts by weight Sorbitan sesquistearate 2 parts by weight Polyoxyethylene (20) behenyl ether 2 parts by weight Vitamin A acid 1 part by weight b) 1,3-butanediol 5 parts by weight Extract powder 2 0.1 part by weight Carboxyvinyl polymer 0.3 part by weight Water 40 parts by weight c) Water 36.4 parts by weight Potassium hydroxide 0.2 parts by weight

Example 8 A tablet (health food) was prepared according to the following formulation. That is, a fluidized-bed granulation was performed while spraying 10 parts by weight of a 50% aqueous ethanol solution to the prescription component, followed by air-drying at 40 ° C., followed by tableting to obtain a tablet. Hydroxypropyl cellulose 8 parts by weight Catalase protective agent 26 10 parts by weight Crystalline cellulose 40 parts by weight Lactose 30 parts by weight Starch 12 parts by weight

Example 9 A tablet (health food) was prepared according to the following formulation. That is, a fluidized-bed granulation was performed while spraying 10 parts by weight of a 50% aqueous ethanol solution to the prescription component, followed by air-drying at 40 ° C., followed by tableting to obtain a tablet. Hydroxypropyl cellulose 8 parts by weight Catalase protective agent 20 10 parts by weight Crystalline cellulose 40 parts by weight Lactose 30 parts by weight Starch 12 parts by weight

Example 10 According to the following formulation,
Tablets (health foods) were prepared. That is, 50%
The fluidized-bed granulation was performed while spraying 10 parts by weight of an aqueous ethanol solution, followed by air-drying at 40 ° C., followed by tableting to obtain tablets. Hydroxypropyl cellulose 8 parts by weight Catalase protective agent 12 10 parts by weight Microcrystalline cellulose 40 parts by weight Lactose 30 parts by weight Starch 12 parts by weight

[0033]

Industrial Applicability The catalase protective agent of the present invention is excellent in the protective effect of catalase for eliminating hydrogen peroxide, and is effective in preventing and improving wrinkles and the like caused by accumulation of hydrogen peroxide.
It can prevent skin aging and maintain a youthful skin condition.

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification code FI Theme coat ゛ (Reference) C12N 9/08 C12N 9/08 (72) Inventor Noriaki Oka 1234 Aino, Fukuroi-shi, Shizuoka Pref. F-term in company development laboratory (reference) 4B018 LE01 MD61 MD66 ME10 MF01 4B050 CC10 KK20 LL02 4C083 AA111 AA112 AB032 AC022 AC072 AC102 AC122 AC182 AC302 AC442 AC482 AD092 AD202 AD242 AD262 AD282 AD622 BB51 CC01 CC04 CC05 CC19 DD12 DD23310101 FF88 AA18 AB12 AB19 AB26 AB34 AB38 AB40 AB47 AB48 AB51 AB62 AB71 AB73 AC03 AC04 AC05 AC11 BA08 CA03 MA63 NA13 ZA89

Claims (7)

[Claims] Claims: 1. An essence of a plant of the family Asteraceae, Mulaceae, Cucurbitaceae, Labiatae, Poaceae, Rosaceae, Citrusaceae, Honeysuckle, Euphorbiaceae, Gamaceae, Echinaceae, Aceraceae or Apiaceae, Catalase protectant. 2. The plant of the family Asteraceae is Anthemisia.
Genus, Artemisia genus, Saponaria genus, H
genus lianthus, genus Achillea, Stev
ia genus, Mulaceae plant is Morus genus, Cucurbitaceae plant is Luffa genus, Lamiaceae plant is Lavandula genus,
Genus Isodon, genus Thymus, genus Perilla,
Gramineae plants of the genus Panicum, Prunus, C
Itrus and honeysuckle plants are Lonicera
A genus and a plant of the genus Euphorbiaceae are Equisetum genus, a plant of the cattail family is a genus of Typha, and a plant of the family Asteraceae is Houttu.
Ymia spp., Lithosperm plant
The catalase protecting agent according to claim 1, wherein the plant belonging to the genus um or the carrot belongs to the genus Panax. 3. The source plant of the essence is chamomile, mulberry, loofah, lavender, artemisia, sunflower, barnyard grass, chickpea, peach savonsou, yarrow, orange, honeysuckle, horsetail, yarrow,
The catalase protective agent according to claim 1, wherein the catalase protective agent is selected from the group consisting of daidai, perilla, lemon, kogama, dokudami, gypsophila, stevia, murasaki and carrot. 4. A composition for preventing aging, comprising one or two or more selected from the catalase protective agents according to claim 1. 5. The composition according to claim 4, which is a food or a cosmetic. 6. The composition according to claim 4, which is a cosmetic. 7. The composition according to claim 4, wherein the aging involves light irradiation.