JP2001097844A - Anti-aging agent and cosmetic containing the same and used for preventing aging - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an anti-aging agent which can eliminate hydrogen peroxide giving a large effect to the aging of skin, can prevent the aging of the skin, and can suitably be used for basic cosmetics, makeup cosmetics, hair cosmetics or bathing agents. SOLUTION: This anti-aging agent contains the essence of Thymus serphyllum. The essence of Thymus serphyllum is anti-aging agent having an excellent hydrogen peroxide-eliminating activity. The anti-aging cosmetic containing the same has excellent improving and preventing effects on the skin aging related to the hydrogen peroxide.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an anti-aging agent and an anti-aging cosmetic containing the same, and more particularly to an anti-aging agent which eliminates hydrogen peroxide which greatly affects skin aging to prevent skin aging. It relates to a possible anti-aging agent. The anti-aging agent of the present invention can be suitably used for basic cosmetics, makeup cosmetics, hair cosmetics, bath additives, and the like.

[0002]

2. Description of the Related Art Changes associated with aging of the skin, ie, wrinkles, dullness, disappearance of texture,
It is known that ultraviolet rays greatly contribute to the decrease in elasticity and the like. Catalase present in the skin is easily inactivated when the skin is irradiated with ultraviolet rays, so it is not difficult to guess that hydrogen peroxide accumulates in the skin during ultraviolet irradiation. It has also been reported that the catalase activity in the skin decreases with aging, and it is considered that skin aging and hydrogen peroxide are closely related. Hydrogen peroxide is not only a long-lived reactive oxygen species, but also a precursor of hydroxyl radical, which is said to be the most harmful of radical species, and also acts as a signal for melanin production and apoptosis. Have been. On the other hand, it has been confirmed that a large amount of hydrogen peroxide is generated from leukocytes migrating into the skin during inflammation, and is considered to be one cause of tissue destruction during inflammation. Accumulation of hydrogen peroxide, which is harmful to the skin during ultraviolet irradiation or with aging, is considered to be one of the major factors of skin aging, and eliminating hydrogen peroxide prevents skin aging. Is considered important. However, most of the conventional anti-aging agents focus on superoxide, hydroxyl radicals, or antioxidants, and there are few that focus on elimination of hydrogen peroxide. Therefore, in order to develop a more effective anti-aging agent, we have developed an anti-aging agent having a scavenging activity for hydrogen peroxide. Therefore, an object of the present invention is to provide a highly safe anti-aging agent that is excellent in preventing and improving aging of the skin, and
It is an object of the present invention to provide an anti-aging cosmetic composition for preventing and improving skin aging containing the same.

[0003]

Means for Solving the Problems In order to solve the above problems, the present inventors have intensively searched for a substance having an anti-aging effect based on hydrogen peroxide scavenging activity. The present inventors have found hydrogen oxide erasing activity and completed the present invention.

That is, the present invention relates to an anti-aging agent containing an extract of Paramecium officinalis and an anti-aging cosmetic containing the anti-aging agent.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail. First, the anti-aging agent of the present invention will be described.

(1) Anti-aging agent of the present invention The anti-aging agent of the present invention comprises an extract of Paramecium officinalis.

[0007] Aspergillus niger has an activity to scavenge hydrogen peroxide and contains a component for preventing skin aging, and the anti-aging agent of the present invention only needs to contain such a component. Therefore, the anti-aging agent of the present invention may be obtained by cutting, crushing, pulverizing, or the like of Ibuki millefon per se, but preferably contains an extract obtained by extracting the above component with a solvent as an active ingredient. . The above-mentioned components can be obtained without any limitation as long as they are a part or all of the plant of Ibuki-mushroom, and examples thereof include roots, stems, leaves, flowers, and seeds. These may be used alone, or two or more parts may be used. Examples of the solvent used for extraction include water, alcohols such as methanol and ethanol, 1,3-butanediol, propylene glycol and glycerin, esters such as ethyl acetate and methyl formate, nitriles such as acetonitrile, diethyl ether and the like. Examples thereof include ethers such as tetrahydrofuran, halogenated hydrocarbons such as chloroform and methylene chloride, and ketones such as acetone and methyl ethyl ketone. One or more of these may be used alone or as a mixture. Most preferred of these are alcohols. The extraction may be performed, for example, by adding a solvent in an amount of 2 to 10 times the amount of the dried plant material or its crushed product, and immersing it for several days at room temperature or for several hours at a temperature near the boiling point. Thereafter, unnecessary substances may be removed by filtration or the like, followed by concentration under reduced pressure. Further, this may be purified by column chromatography using a column filled with silica gel, ODS, ion exchange resin and the like.

(2) Anti-aging cosmetic containing the anti-aging agent of the present invention The anti-aging cosmetic of the present invention contains the above-mentioned anti-aging agent.

The cosmetics to which the present invention is applied have no particular limitation on the dosage form and application. For example, the dosage form may be any of a solution, an emulsion, a cream, an aqueous gel, and the like. In addition to the base material, there may be mentioned finishing materials such as foundations and control colors. These cosmetics can be manufactured by the same method as ordinary cosmetics except that the above-mentioned anti-aging agent is blended.

The compounding agent of the above-mentioned anti-aging agent is not particularly limited, but it is desirable that the compounding agent is added to cosmetics in the range of 0.005 to 1% by weight. The cosmetic of the present invention includes various components generally used in cosmetics, that is, an aqueous component, an oily component, a powder component, a surfactant, a humectant, a thickener, a coloring agent, a fragrance, an antioxidant, and a pH adjuster. , A chelating agent, a preservative, or an ultraviolet protective agent, an anti-inflammatory agent, a whitening agent, and the like.

[0011]

Embodiments of the present invention will be described below. The percentages used below are by weight unless otherwise specified.

<Example 1> 5 l of a 50% ethanol aqueous solution was added to 3 kg of the upper part of the dough of Ibuki gypsophila, and the mixture was refluxed under heating for 2 hours, filtered to remove unnecessary substances, and concentrated under reduced pressure to obtain 28 g of anti-aging agent 1 of the present invention. .

Example 2 28 g of anti-aging agent 1 was added to 1 l of water
The column was charged with DIAION HP-20, washed by flowing 2 l of water, and eluted by flowing 5 l of a 50% aqueous ethanol solution. This was concentrated to obtain 18 g of anti-aging agent 2.

Example 3 3 kg of dried whole plant of Ibuki gypsophila was cut into small pieces, and 20 l of 50% ethanol was added thereto.
The mixture was refluxed for a period of time, filtered, and concentrated under reduced pressure.
Was obtained in an amount of 72 g.

<Example 4> Measurement of anti-aging effect (measurement of hydrogen peroxide scavenging activity)
Vitro) A conventional rhodamine fluorescence method is used. Non-fluorescent rhodamine is oxidized by peroxidase in the presence of hydrogen peroxide to emit fluorescence. Since the fluorescence intensity is proportional to the amount of hydrogen peroxide, the hydrogen peroxide scavenging activity was measured using this principle.

Hydrogen peroxide solution adjusted to 2 μM 500 μM
1 in a light-shielding bottle, 500 μl of an anti-aging agent solution containing 1 μg of the anti-aging agent of the present invention, 6.4 units of horseradish peroxidase (SIGMA P8000)
1000 μl of DHR123 (manufactured by Wako Pure Chemical Industries, Ltd.) adjusted to / 1000 μl and 9 μM was added, and the mixture was incubated at 37 ° C. in a dark place for 30 minutes, and then the excitation wavelength 488 nm and the fluorescence wavelength 530 nm were measured. All were dissolved in 0.1 M phosphate buffer (pH 7.4). As a standard, a test group to which only 500 μl of a buffer solution (pH 7.4) containing no anti-aging agent was added was prepared. As a control, gallic acid or fresh lavender leaves were treated in the same manner as anti-aging agent 1 μg / 5.
A test plot to which 00 μl was added was prepared.

The results are shown in Table 1. The hydrogen peroxide erasing activity was determined as 100% when all the hydrogen peroxide was erased. From these results, it can be seen that the anti-aging agent of the present invention, the extract of Paramecium officinalis, has excellent hydrogen peroxide scavenging activity.

[0018]

[Table 1]

Example 5 Five groups of hairless mice were used to examine the effects of ultraviolet radiation on aging in a photoaging model. That is, a hairless mouse is treated by applying 0.1 ml of a 50% aqueous ethanol solution containing 0.1% of extract powder of anti-aging agent 2, and then applying ultraviolet rays (BLB lamp; Toshiba Corporation, 1/3 times the amount of MED) Manufactured).
This operation was performed 8 times at a rate of 5 times / week for 8 weeks, and the state of elasticity of the skin was compared with the average level of the control group not subjected to ultraviolet irradiation and sample administration.
+: Elasticity, ±: slightly reduced elasticity,-: markedly reduced elasticity, and ++: similar to dullness of skin color
Almost no dullness, +: dullness is suppressed, ±:
The evaluation was based on the criteria that slight dullness was suppressed,-: dullness was not suppressed. As a comparative control, a group to which a 50% aqueous ethanol solution containing 0.1% of powder obtained by treating gallic acid or lavender fresh leaves in the same manner as the anti-aging agent 2 was provided. Table 2 shows the results. From this, it can be seen that the extract of Paramecium officinalis has an effect of preventing aging caused by light in a photoaging model.

[Table 2]

<Example 6> Five groups of hairless mice were used to examine the effect of ultraviolet radiation on the aging inhibition in a photoaging model. That is, 1/3 times the amount of ultraviolet light (BLB
After irradiation with a lamp (manufactured by Toshiba Corporation), 0.1 ml of a 50% aqueous ethanol solution containing 0.1% of an extract powder of anti-aging agent 2 was applied and post-treated. This work 5 times / 1
Performed at a weekly rate for 8 weeks, and the state of skin elasticity was compared with the average level of the control group not subjected to ultraviolet irradiation and sample administration, ++: very elastic, +: elastic,
±: Slight loss of elasticity,-: Standard for severe loss of elasticity. Also, the dullness of skin color is similarly ++: Almost no dullness, +: Dullness is suppressed, ±: Slight dullness is suppressed Done,-: Evaluation was made based on the criteria that dullness was not suppressed. As a comparative control, a group to which a 50% aqueous ethanol solution containing 0.1% of powder obtained by treating gallic acid or lavender fresh leaves in the same manner as the anti-aging agent 2 was provided. Table 3 shows the results. From this, it can be seen that the extract of Aspergillus niger according to the present invention suppresses aging even after administration after ultraviolet irradiation. From this, it can be seen that the effect of Example 5 is not an effect due to the ultraviolet absorbing effect or the like, but is a substantial anti-aging effect.

[Table 3]

Example 7 A lotion was prepared according to the following formulation. That is, the ingredients were stirred and solubilized at room temperature to obtain a lotion. This lotion is used twice a day for one month in the morning and evening by using a group of three panelists suffering from dullness, often exposed to ultraviolet rays during work outside in the summer, to evaluate the effect of preventing and improving the dullness. I was asked to. Evaluation criteria are
Rating 2: marked prevention / improvement, rating 1: obvious prevention / improvement, rating 0.5: slight prevention / improvement, rating 0: no improvement. As a control, the extract powder of the anti-aging agent 2 was replaced with water, and for comparison, the extract powder of the anti-aging agent 2 was replaced with a lavender fresh leaf extract powder extracted in the same manner as the anti-aging agent 2. Was used. Table 4 shows the results. From this, it was confirmed that the lotion containing the anti-aging agent of the present invention is effective in preventing and improving aging such as dullness.

[0022]

[Table 4]

Example 8 A cream was prepared according to the following recipe. That is, (a), (b) and (c) were each heated and dissolved at 80 ° C., (b) was gradually added to (a), and (c) was further added and emulsified. The homogenized particles were homogenized by a homomixer and cooled to obtain a cream.

A) Squalane 10 parts by weight Cetanol 3 parts by weight Sorbitan sesquistearate 2 parts by weight Polyoxyethylene (20) behenyl ether 2 parts by weight Vitamin A acid 1 part by weight b) 1,3-butanediol 5 parts by weight Extract powder 2 0.1 part by weight Carboxyvinyl polymer 0.3 part by weight Water 40 parts by weight c) Water 36.4 parts by weight Potassium hydroxide 0.2 parts by weight

[0025]

The anti-aging agent of the present invention has an excellent effect of eliminating hydrogen peroxide, and is effective in preventing and improving wrinkles and the like caused by accumulation of hydrogen peroxide, preventing aging of the skin, It can maintain youthful skin condition.

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Noriaki Oka 1234 Aino, Fukuroi-shi, Shizuoka Pref. EE12 4C088 AB38 AC02 BA09 BA10 CA03 MA17 MA28 MA63 NA14 ZA89 ZC52

Claims (3)

[Claims] 1. Thymus scutellaria (Thymus s)
erphyllum). 2. The anti-aging agent according to claim 1, wherein the essence is a solvent extract. 3. An anti-aging cosmetic comprising the anti-aging agent according to claim 1.