JPH1072336A - Collagen gel contraction accelerator - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a collagen gel contraction accelerator having excellent improving effect on loosening of skin and tightening effect on skin. SOLUTION: Each of this collagen gel contraction accelerator, a composition for improving loosening of skin and a composition for tightening of skin, contains one or more than two kinds of plants or their extracts selected from Ginkgo biloba, Rhodophyceae, Fucales fucus, Matricaria chamomilla, Perilla frutescens var. acuta, Persica semen, Panax ginseng, Foeniculum vulgare, Morus bombycis, Gentiana lutea, Arctium lappa, Cortinellus shiitake, Allium sativum, Humulus lupulus, Paeonia suffruticosa, Saxifrage stolonifera, Lactuca sativa, Poria hoelen, Rosmarinus officinalis, Astragalus sinicus, Actinidia chinensis, Salvia officinalis and Filipendula multijuga as active components.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a collagen gel shrinkage promoter, a composition for improving skin elasticity, skin elasticity, skin elasticity and skin firmness, and a composition for tightening skin.

[0002]

2. Description of the Related Art The skin is mainly divided into three layers, epidermis, dermis, and subcutaneous tissue. Of these, the dermis is extremely important for maintaining the structure of the skin, and is made of fibers such as collagen and elastin to be firm and flexible. , Forming the dermis connective tissue. Proteins such as collagen and elastin which constitute fibers of connective tissue are synthesized / degraded by fibroblasts. Fibroblasts control the state of connective tissue by interacting with these fibers such as collagen.

When fibroblasts are embedded and cultured in a collagen gel under normal fibroblast culture conditions, the collagen gel contracts (Review: E. Bell et al. J. Invest. Dermat).
ol., 81, 2s (1983)). The contraction of the collagen gel differs depending on the number of cells and the amount of serum in the medium, and becomes more remarkable as the number of fibroblasts in the gel increases and the amount of serum in the medium increases. However, such contraction can be caused by collagen-only gels without fibroblasts,
This does not occur at all in gels containing floating cells such as lymphocytes.

With respect to skin aging, it is known that gel contraction of fibroblasts derived from the aged is reduced as compared with cells derived from the young. (M. Yamato, et a
l. Mech. Aging Dev. 67, 149 (1993)). The reduced contractility of the collagen gel indicates that it is difficult to contract the dermis connective tissue as before, and maintain it in a tight state. In fact,
For example, it is a well-known fact that as the skin ages, tarmi, which is rarely observed when youth, is formed on the skin of the cheeks, neck, arms, and other parts.

[0005] From the above, it is considered that the dermis connective tissue of the skin loses the contracting force, and further loses the strength and elasticity, resulting in tarmi. Therefore, if the collagen gel with fibroblasts embedded therein can be enhanced in contractility, and if the formation strength of the gel can be increased, the dermis connective tissue can be further contracted, tightened, and increased in strength, thereby increasing the strength of It is thought that such a state can be maintained. It is also thought that aging improves skin bulging, reduced skin elasticity and firmness, can tighten skin, and can also promote healing of wounds caused by destruction of dermal connective tissue. . Under such circumstances, development of a collagen gel shrinkage promoter has been desired.

[0006] Conventionally, substances that promote contraction of collagen gel embedded in fibroblasts include serum and endothelin (C. Guidry et al. J. Cell Biol., 115, 873).
(1991)), transforming growth factor β (R. Montesano et al. Pro. Natl, Acad. Sci. US
A, 85, 4894 (1988), EM Grant et al.
J. Cell Sci., 102, 315 (1992)), growth promoting factors such as platelet growth factor, and compounds such as retinoic acid are known, but they have problems in transdermal absorption, stability, safety, and price. And the effect was not enough.

[0007]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a collagen gel shrinkage-promoting agent excellent in transdermal absorbability, stability, safety and price, a composition for improving skin elasticity, skininess and firmness. And a skin tightening composition.

[0008]

In view of such circumstances, the present inventors have conducted intensive studies on a substance having a collagen gel contraction promoting effect using a human skin fibroblast collagen-embedded culture system. The present inventors have found that extracts and the like have a collagen gel shrinkage promoting effect, and further found that they are useful as a composition for improving skin elasticity, tallness, firmness, and a composition for tightening skin, thereby completing the present invention. Reached.

That is, the present invention relates to ginkgo, ginseng, hibamata, chamomile, perilla, tonin, carrot,
One or more plants or extracts thereof selected from fennel, mulberry, gentian, burdock, shiitake mushroom, garlic, hop, buttonpi, yukinoshita, lettuce, boukuro, rosemary, vetch, kiwi, salvia and sycamore It is intended to provide a collagen gel shrinkage promoter. Further, the present invention provides a composition for improving skin ulcer and a composition for tightening skin, which contain a collagen gel contraction promoter.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION Various plants or extracts thereof used in the present invention are those already known as general skin external preparations, cosmetics, raw materials for pharmaceuticals, base materials and additives. It is known that a certain plant or its extract has effects such as a moisturizing effect, an anti-inflammatory effect, a blood circulation promoting effect, a hair nourishing effect, and a whitening effect. However, its collagen gel shrinkage promoting effect, furthermore, skin elasticity, tallness, firmness improving effect, and skin tightening effect were not known at all. In addition, among the plants used in the present invention, gypsophila and hibamata are one kind of seaweed.

The plant used in the present invention refers to one or more of the whole plant (or whole algae) or their leaves, petiole, stem, root, and seed (hereinafter, referred to as "prototype"). Or, it is obtained by drying and pulverizing, and the plant extract is a substance that is dried or pulverized without drying, and then extracted with a solvent at room temperature or under heating, or extraction with a Soxhlet extractor or the like. It means various solvent extracts obtained by extraction using an instrument, their diluents, their concentrated solutions, or their dried powders.

The solvent used for the extraction includes water, an organic solvent and a mixture thereof, and an organic solvent or a mixture of water and an organic solvent is particularly preferable. Preferred specific examples of the organic solvent include hydrocarbons such as petroleum ether, cyclohexane, toluene and benzene; halogenated hydrocarbons such as dichloromethane, chloroform and carbon tetrachloride; esters such as ether and ethyl acetate; ketones such as acetone. Species: methanol, butanol, propanol,
Alcohols such as ethanol, polyethylene glycol, propylene glycol and butylene glycol; and pyridine.

When, for example, aqueous alcohol is used as the organic solvent, a plant extract can be obtained by subjecting the above plant to an extraction treatment at 3 to 70 ° C. As a specific example of a preferable extraction method from the drug substance, 5000 g of 70% v / v ethanol is added to 1000 g of the dry and pulverized product, and the mixture is extracted for 7 days with occasional stirring at room temperature. The obtained extract is filtered, After allowing the filtrate to stand at 5 ° C. for 3 days, it is filtered again to obtain a plant extract as a supernatant. The obtained plant extract can be used as it is as an active ingredient of the drug of the present invention. After diluting, concentrating, or freeze-drying the extract, it is prepared in powder or paste form, and is appropriately formulated and used. Can also. Further, if necessary, it may be used after subjecting it to a purification treatment such as deodorization and decolorization by a known method. Plant extracts
A product extracted in this manner may be used, or a commercially available product may be used.

The above-mentioned plant or its extract can be used as it is as a collagen gel contraction promoter, but it can also be appropriately formulated and used. The collagen gel contraction promoter can be administered by any method of external use and internal use, but external administration is preferred, and a composition for improving skin ulcer or a skin tightening composition containing the same is used as a skin external preparation. Preferably, it is in the form.

The amount of the plant or the extract thereof in the composition of the present invention is preferably 0.00001 to 10% by weight, usually 0.000001 to 10% by weight, as a dry solid content of the active ingredient, from the viewpoint of effect, mixability and feeling of use. -3% by weight is particularly preferred.

The composition containing the collagen gel shrinkage-promoting agent of the present invention may contain, in addition to the plant or its extract, a commonly used external base material and other medicinal ingredients. The external base material used herein may be any of those based on an oily base, those based on an oil / water or water / oil type emulsified base, and those based on water. .
The oily base is not particularly limited, and includes, for example, vegetable oil, animal oil, synthetic oil, silicone oil, fatty acid, natural or synthetic glyceride, and the like. In addition, humectants, ultraviolet absorbers, alcohols, chelates, pH adjusters, preservatives,
Thickeners, pigments, fragrances and the like can be arbitrarily combined and compounded. The medicinal component is not particularly limited, and, for example, an analgesic / anti-inflammatory agent, a disinfectant / antiseptic agent, vitamins, a skin emollient, and the like can be used as needed. Examples of the form of the skin external preparation composition include ointments, creams, emulsions, lotions, gels, packs, cataplasms, foundations and the like.

[0017]

Next, the present invention will be described in detail with reference to examples, but the present invention is not limited to these examples.

Production Example 1 1 kg of the pulverized material at the site shown in the following table of each plant was immersed in 5 liters of an extraction solvent at room temperature for 1 week to extract a solvent-soluble component.
The same operation is repeated for the residue obtained by separating the extract,
A total of 10 liters of extract was obtained. The solvent of this extract was distilled off and dried under reduced pressure to obtain an extract. In the following, BG indicates 1,3-butylene glycol, ET indicates ethanol, and MT indicates methanol.

[0019]

[Table 1]

Test Example 1 Measurement of Collagen Gel Shrinkage Accelerating Ability Collagen gel is described in J. Cell Science, 102 , 31
5 (1992) "or" J. Invest. Dermatol, 93 ,
792 (1989) ". That is, a collagen gel solution (manufactured by Nitta Gelatin Co.,
HEPES, (250m) to peI-A (3.0mg / ml, pH = 3)
M) in 0.05N sodium hydroxide solution, DMEM (GIBCO
DMEM, low glucose) 5 times concentrated solution, FCS (5%, Fatal
Calf Serum) and purified water were added, and the mixture was thoroughly stirred and neutralized. After that, the final concentration of the test substance was 0.01 to 0.001% by weight (dry solid weight%) (the plant extract obtained in Production Example 1 and the control). Ethanol or 1,3-butylene glycol), and finally, a suspension of human dermal fibroblasts (derived from human foreskin) was added, and the mixture was sufficiently stirred to remove air bubbles. Injected and immediately gelled at 37 ° C. At this time, the collagen concentration was adjusted to 1.5 mg / ml. Twenty-four hours later, the periphery of the gel was peeled off, a medium was added, and the gel was cultured.

Gel diameter measurement is described in the literature (Arch. Dermatol.
Res, 280, 114 (1988)). That is, the diameter was measured from three directions on graph paper, and the measured values were averaged. Gel volume measurement is described in the literature (J. Cell Scie
nce, 102, 315 (1992)). That is, 10% formalin fixation (4 ° C, 2
After 4 hours), the surface tension of water was reduced by adding Triton X100 (manufactured by Wako Pure Chemical Industries, Ltd.) (1%), and the weight was measured.

The results are shown in Table 2. As is apparent from Table 2, the action of various plant extracts reduces the diameter and volume of the collagen gel, and promotes the contraction of the collagen gel.

[0023]

[Table 2]

Test Example 2 Evaluation of Talmi Improvement The evaluation of the Talmi improvement agent was performed using an experimental system using a hairless mouse (HR mouse) as a collagen gel contraction promoter. Approximately 10 months old H which has caused considerable skin thickening
Each plant extract solution (ethanol or water / ethanol solution) and ethanol as a control were continuously applied to the back of the R mouse (HR / ICR) once a day for 8 weeks. The evaluation of the tarmi condition was performed by a visual score of the tarmi and a method of measuring the time until the back skin was picked up with a finger, released, and returned (pinch test). The index of the Talmi visual score was set as follows. Table 3 shows the results.

Score 1: The skin is not loosened Score 2: The skin is slightly loosened Score 3: The skin is slightly loosened Score 4: The skin is loosened

As is evident from Table 3, the collagen gel contraction promoter selected from the present evaluation system improves the skin on the back skin of HR mice.

[0027]

[Table 3]

The skin external preparation compositions of Examples 1 to 25 were prepared. In the following, dry solids among plant extracts are
The dry solid obtained in Production Example 1 and shown together with the solvent are those that were blended with the solvent without distilling off the extraction solvent (the numerical values are% by weight in terms of dry solid).

Example 1 Cream

[0030]

(Ingredients) (% by weight) Stearic acid 2.00 Squalane 2.00 Cholesterol 3.00 Olive oil 1.00 Cetanol 7.00 Jojoba oil 2.00 2-Hexadecylphosphate arginic acid 2.00 Polyoxy Ethylene (40EO) hydrogenated castor oil 0.50 Glycerin 10.00 1,3-butylene glycol 5.00 Hibamata extract (BG) 10.00 Carrot extract (50% water / ET) 2.00 Tonin extract (dry solid) 0 .05 Gentian extract (dry solid) 0.05 Succinic acid 1.00 Purified water Balance meter 100.00

Example 2 Gel

[0032]

(Components) (% by weight) Polyacrylic acid (Carbopol, manufactured by Goodrich Co.) 0.50 Potassium hydroxide 0.15 Glucam 10.00 86% Glycerin 10.00 Glycinebetaine 3.00 Succinic acid 1 .50 Hibamata extract (BG) 5.00 Carrot extract (50% water / ET) 5.00 Purified water Balance meter 100.00

Example 3 Gel

[0034]

(Components) (% by weight) Polyoxyethylene isocetyl ether (20EO) 1.00 Polyoxyethylene sodium trialkylphosphate (10EO) 1.00 Sorbitol 0.50 1,3-propanediol 0.50 Xanthan gum 0.50 Tuberose polysaccharide 3.00 Carboxyvinyl polymer (Carbopol 940, manufactured by Goodrich) 0.20 Potassium hydroxide 0.90 Allantoin 0.10 Zinc sulfocarbonate 0.20 Tonin extract (50% water / ET) 1.00 Gentiana extract (50% water / ET) 3.00 Ginkgo extract (BG) 3.00 Ethanol 20.00 Preservatives Appropriate perfume Appropriate amount Purified water Balance meter 100.00

Example 4 Gel

[0036]

(Components) (% by weight) Silicone composition (XSG-16, manufactured by Shin-Etsu Chemical Co., Ltd.) 5.00 Methyl polysiloxane (KF96A-6cs, manufactured by Shin-Etsu Chemical Co., Ltd.) 15.00 Methyl polysiloxane (SH244, Toray) -Dow Corning Co.) 5.00 Methyl polysiloxane-methyl (polyoxyethylene) siloxane copolymer (SH3771C, Dow Corning Toray) 2.00 Methyl polysiloxane-methyl (polyoxyethylene) siloxane copolymer (SH3775C, Dow Corning Toray Co., Ltd.) 1.00 Methylcellulose 0.20 Hydroxyethylcellulose hydroxypropyltrimethyl ammonium chloride ether (Caticello H-60, manufactured by Kao Corporation) 0.02 dl-α-tocopherol acetate 0.05 Glycyrrhetinic acid iso Stearyl 0.10 Isopropyl methylphenol 0.10 Sub Lead flower 1.50 Carrot extract (50% water / ET) 1.00 Fennel extract (50% water / ET) 1.00 Hibamata extract (BG) 5.00 Rosemary extract (dry solid) 0.05 Ethanol 5 .00 Preservatives Appropriate amount Fragrance Appropriate amount Purified water Balance meter 100.00

Example 5 Gel

[0038]

Table 8 (Components) (% by weight) Polyoxyethylene isocetyl ether (20EO) 1.00 Polyoxyethylene sodium trialkylphosphate (10EO) 1.00 Sorbitol 0.50 1,3-propanediol 0.50 Xanthan gum 0.50 Tuberose polysaccharide 3.00 Carboxyvinyl polymer (Carbopol 940, Goodrich) 0.20 Dipotassium glycyrrhizinate 0.10 Potassium hydroxide 0.90 Allantoin 0.10 Tannic acid 0.20 Buttonpig Extract (50% water / ET) 10.00 Fennel extract (50% water / ET) 5.00 Garlic extract (ET) 0.10 Burdock extract (water) 0.50 Ethanol 20.00 Preservatives Appropriate perfume Appropriate amount of purified water Balance meter 100.00

Example 6 Gel

[0040]

Table 9 (Components) (% by weight) Silicone composition (XSG-17, manufactured by Shin-Etsu Chemical Co., Ltd.) 5.00 Methyl polysiloxane (KF96A-6cs, manufactured by Shin-Etsu Chemical Co., Ltd.) 15.00 Methyl polysiloxane (SH244, Toray) -Dow Corning Co.) 5.00 Methyl polysiloxane-methyl (polyoxyethylene) siloxane copolymer (SH3771C, manufactured by Dow Corning Toray) 2.00 Methyl polysiloxane-methyl (polyoxyretylene) siloxane copolymer Coalescing (SH3775C, manufactured by Dow Corning Toray) 1.00 Methylcellulose 0.20 Hydroxyethylcellulose hydroxypropyltrimethyl ammonium chloride ether (Caticello H-60, manufactured by Kao Corporation) 0.02 dl-α-tocopherol acetate 0.05 Glycyrrhetinic acid Isostearyl 0.10 Isopropylmethylphenol 0.10 E TA 0.10 Hibamata extract (70% water / ET) 10.00 Carrot extract (water) 3.00 Tonin extract (50% water / ET) 2.00 Ginkgo extract (BG) 2.00 Ethanol 5.00 Preservative Perfume Appropriate amount of purified water Balance meter 100.00

Example 7 Lotion

[0042]

(Component) (% by weight) Arginine 0.40 Lysine 0.40 Polyoxyethylene hydrogenated castor oil (40EO) 1.50 Methylpolysiloxane / methyl (polyoxyethylene) siloxane copolymer (SH3775C, Toray Co., Ltd.) 0.60 glycerin 5.00 glycine betaine 0.20 trisodium citrate 0.90 citric acid 0.40 urea 0.50 ε-aminocaproic acid 0.10 chamomile extract (dry solid) 0.05 Red bean extract (dry solid) 0.10 Shiitake extract (water) 2.00 Lettuce extract (BG) 2.00 Ethanol 5.00 Preservatives Appropriate amount Perfume Appropriate amount Purified water Balance meter 100.00

Example 8 Lotion

[0044]

Table 11 (Components) (% by weight) Triethanolamine 0.40 Potassium hydroxide 0.20 Polyoxyethylene isocetyl ether (20EO) 0.30 Polyoxyethylene oleyl ether sodium phosphate (8EO) 0.30 Poly Sodium oxyethylene dialkyl phosphate (10EO) 0.10 Polyethylene glycol 1500 2.00 Polyoxyethylene methyl glucoside (10EO) 1.50 Dipropylene glycol 0.50 Disodium hydrogen phosphate 0.50 Succinic acid 0.30 Hiba Mata extract (Dry solid matter) 0.10 Carrot extract (50% water / ET) 2.00 Red carrot extract (50% water / ET) 2.00 Ginkgo extract (50% water / ET) 2.00 Ethanol 10.00 Preservative Perfume Appropriate amount of purified water Balance meter 100.00

Example 9 Packing agent

[0046]

(Ingredient) (% by weight) Polyvinyl alcohol (Gohsenol EG-30, manufactured by Nippon Synthetic Chemical Industry Co., Ltd.) 11.70 Glycerin 1.00 Titanium oxide 1.50 Hibamata extract (water) 15.00 Carrot extract (BG ) 5.00 Tonin extract (70% water / ET) 5.00 Ginkgo extract (dry solid) 0.05 Tartaric acid 7.00 Purified water Balance meter 100.00

Example 10 Packing agent

[0048]

[Table 13] (Components) (% by weight) Polyvinyl alcohol (Gohsenol EG-30, manufactured by Nippon Gohsei) 15.00 Carboxymethylchitin aqueous solution (chitin liquid HV-10, manufactured by Ichimaru Falcos) 5.00 Triglucopolysaccharide ( 0.50 xanthan gum 0.50 sodium carboxymethylcellulose 0.50 titanium oxide 15.00 aluminum magnesium silicate 1.00 1-isostearoyl-3-myristoyl-glycerol 1.00 diglycerin 1. 50 Polyoxyethylene isocetyl ether (20EO) 1.00 Gentian extract (50% water / ET) 10.00 Hibamata extract (BG) 3.00 Chamomile extract (50% water / ET) 3.00 Rosemary extract (50 % Water / ET) 1.00 ethanol 5.0 Preservative qs Perfume qs Purified water Balance Total 100.00

Example 11 Packing agent

[0050]

(Components) (% by weight) Polyvinyl alcohol (Gohsenol EG-30, manufactured by Nippon Gohsei) 15.00 Carboxymethyl chitin aqueous solution (chitin liquid HV-10, manufactured by Ichimaru Falcos) 5.00 Triglucopolysaccharide ( 0.50 xanthan gum 0.50 sodium carboxymethylcellulose 0.50 titanium oxide 15.00 aluminum magnesium silicate 1.00 1-isostearoyl-3-myristoyl-glycerol 1.00 diglycerin 1. 50 Polyoxyethylene isocetyl ether (20EO) 1.00 Buttonpiex (50% water / ET) 10.00 Mulberry extract (dry solid) 0.10 Hop extract (dry solid) 0.10 Ethanol 5.00 Preservative Qs perfume qs purified water balance meter 100.00

Example 12 Emulsion

[0052]

(Components) (% by weight) Palmitic acid 0.50 Olive oil 2.00 Cetanol 1.00 Jojoba oil 5.00 Monohexadecyl phosphate sodium salt 2.00 Sorbitan monostearate 0.50 Glycerin 15.00 Ethanol 5.00 Hibamata extract (30% water / ET) 5.00 Carrot extract (50% water / ET) 5.00 Chamomile extract (50% water / ET) 1.00 Lactic acid 2.00 Purified water Balance meter 100. 00

Example 13 Emulsion

[0054]

[Table 16] (Components) (% by weight) Arginine 0.40 Lysine 0.40 Polyoxyethylene hydrogenated castor oil (40EO) 1.50 Methylpolysiloxane / methyl (polyoxyethylene) siloxane copolymer (SH3775C, Toray Co., Ltd.) 0.60 maltitol 5.00 ethylene glycol monoethyl ether 0.20 trisodium citrate 0.90 succinic acid 0.40 urea 0.50 ε-aminocaproic acid 0.10 hibamata extract (50% water / ET) 5.00 Ginkgo extract (50% water / ET) 5.00 Buttonpipe extract (BG) 1.00 Ethanol 2.00 Preservatives Appropriate amount Perfume Appropriate amount Purified water Balance meter 100.00

Example 14 Emulsion

[0056]

Table 17 (Components) (% by weight) N- (3-hexadecyloxy-2-hydroxypropyl) -N-2-hydroxyethyldecanamide 0.50 N- (3-hexadecyloxy-2-hydroxy) Propyl) -N-2-hydroxyethyl hexadecanamide 1.00 N- (3-tetradecyloxy-2-hydroxypropyl) -N-2-hydroxyethyldecanamide 0.50 polyoxyethylene hydrogenated castor oil ( 10EO) 1.00 Methyl polysiloxane / methyl (polyoxyethylene) siloxane copolymer (SH3775C, manufactured by Dow Corning Toray) 1.00 Sorbitan monostearate 0.20 Sodium stearoyl methyl taurine 0.50 Cholesterol 0.80 Cholesterol isostearate 0.20 Monocholesteryl alkenyl succinate .80 Stearic acid 0.20 Palmitic acid 0.30 Myristic acid 0.10 Pentyl glycol dicaprate 4.00 Methyl polysiloxane (KF96A-500cs, Shin-Etsu Chemical Co., Ltd.) 2.00 Isostearyl alcohol 1.20 Cetyl alcohol 1. 00 Glycerin 3.50 Lactic acid 0.20 Sodium lactate 0.30 Bompi extract (dry solid) 0.05 Rosemary extract (50% water / ET) 2.00 Tonin extract (100% ET) 1.00 Preservatives Appropriate perfume Appropriate amount of purified water Balance meter 100.00

Example 15 Emulsion

[0058]

Table 18 (Components) (% by weight) N- (3-hexadecyloxy-2-hydroxypropyl) -N-2-hydroxyethyldecanamide 0.50 N- (3-hexadecyloxy-2-hydroxy) Propyl) -N-2-hydroxyethyl hexadecanamide 1.00 N- (3-tetradecyloxy-2-hydroxypropyl) -N-2-hydroxyethyldecanamide 0.50 polyoxyethylene hydrogenated castor oil ( 10EO) 1.00 Methyl polysiloxane / methyl (polyoxyethylene) siloxane copolymer (SH3773C, manufactured by Dow Corning Toray) 1.00 Sorbitan monostearate 0.20 Cholesterol 0.80 Cholesterol isostearate 0.20 Monocholesteryl alkenyl succinate 0.80 Stearic acid 0.50 Squalane 4.0 0 Methyl polysiloxane (XF96A-500cs, manufactured by Shin-Etsu Chemical Co., Ltd.) 2.00 2-Ethylhexyl paramethoxycinnamate (Persol MCX-SA, manufactured by Divaudan) 2.00 Carrot extract (50% water / ET) 5.00 Gentian extract (dry solids) 0.05 Bokuryo extract (70% water / ET) 2.00 Saxifrage extract (dry solids) 0.05 Cetyl alcohol 1.00 Glycerin 3.50 Lactic acid 0.20 Sodium lactate 0.30 Preservatives qs perfume qs purified water balance meter 100.00

Example 16 Patch

[0060]

Table 19 (Components) (% by weight) α-monoisostearyl glycer ether 1.00 pentyl glycol methyl polysiloxane neocaprate 4.00 glycerin 35.00 purified water 15.80 sodium polyacrylate 5.50 potassium aluminum sulfate 1.00 Aqueous solution of polyacrylic acid (10%) 15.00 Light anhydrous silicic acid 2.00 Hibamata extract (50% water / ET) 5.00 Carrot extract (50% water / ET) 2.00 Tonin extract (50% water) / ET) 1.00 Ginkgo extract (dry solid) 0.01 Bulk extract (water) 0.50 Purified water Balance meter 100.00

Example 17 Cream

[0062]

(Ingredients) (% by weight) Stearic acid 2.00 Squalane 2.00 Cholesterol 3.00 Olive oil 1.00 Cetanol 7.00 Jojoba oil 2.00 2-Hexadecylphosphate arginic acid 2.00 Polyoxy Ethylene (40EO) hydrogenated castor oil 0.50 Glycerin 10.00 1,3-butylene glycol 5.00 Hibamata extract (water) 5.00 Salvia extract (50% water / ET) 2.00 Tonin extract (dry solid) 0 .05 Gentian extract (dry solid) 0.05 Succinic acid 1.00 Purified water Balance meter 100.00

Example 18 Gel

[0064]

(Components) (% by weight) Polyacrylic acid (Carbopol, manufactured by Goodrich Co.) 0.50 Potassium hydroxide 0.15 Glucam 10.00 86% Glycerin 10.00 Glycinebetaine 3.00 Succinic acid 1 .50 Hibamata extract (water) 2.00 Carrot extract (50% water / ET) 5.00 Purified water Balance meter 100.00

Example 19 Gel

[0066]

(Component) (% by weight) Polyoxyethylene isocetyl ether (20EO) 1.00 Polyoxyethylene sodium trialkyl phosphate (10EO) 1.00 Sorbitol 0.50 1,3-propanediol 0.50 Xanthan gum 0.50 Tuberose polysaccharide 3.00 Carboxyvinyl polymer (Carbopol 940, manufactured by Goodrich Co.) 0.20 Potassium hydroxide 0.90 Allantoin 0.10 Zinc sulfocarbonate 0.20 Tonin extract (50% water / ET) 1.00 Spiraea extract (50% water / ET) 3.00 Ginkgo extract (BG) 3.00 Ethanol 20.00 Preservatives Appropriate amount Perfume Appropriate amount Purified water Balance meter 100.00

Example 20 Gel

[0068]

(Ingredient) (% by weight) Silicone composition (XSG-16, manufactured by Shin-Etsu Chemical Co., Ltd.) 5.00 Methyl polysiloxane (KF96A-6cs, manufactured by Shin-Etsu Chemical Co., Ltd.) 15.00 Methyl polysiloxane (SH244, Toray) -Dow Corning Co.) 5.00 Methyl polysiloxane-methyl (polyoxyethylene) siloxane copolymer (SH3771C, Dow Corning Toray) 2.00 Methyl polysiloxane-methyl (polyoxyethylene) siloxane copolymer (SH3775C, manufactured by Dow Corning Toray Co., Ltd.) 1.00 Methylcellulose 0.20 Hydroxyethylcellulose hydroxypropyltrimethyl ammonium chloride ether (Caticello H-60, manufactured by Kao Corporation) 0.02 dl-α-tocopherol acetate 0.05 Glycyrrhetinic acid iso Stearyl 0.10 Isopropyl methylphenol 0.10 Sub Lead flower 1.50 Carrot extract (50% water / ET) 1.00 Fennel extract (50% water / ET) 1.00 Hibamata extract (water) 2.00 Rosemary extract (dry solid) 0.05 Ethanol 5 5.00 tartaric acid 7.00 purified water balance meter 100.00

Example 21 Packing agent

[0070]

(Table 24) (Components) (% by weight) Polyvinyl alcohol (Gohsenol EG-30, manufactured by Nippon Gohsei) 15.00 Carboxymethyl chitin aqueous solution (chitin liquid HV-10, manufactured by Ichimaru Falcos) 5.00 Triglucopolysaccharide ( 0.50 xanthan gum 0.50 sodium carboxymethylcellulose 0.50 titanium oxide 15.00 aluminum magnesium silicate 1.00 1-isostearoyl-3-myristoyl-glycerol 1.00 diglycerin 1. 50 polyoxyethylene isocetyl ether (20EO) 1.00 gentian extract (50% water / ET) 10.00 hibamata extract (water) 3.00 chamomile extract (50% water / ET) 3.00 rosemary extract (50 % Water / ET) 1.00 ethanol 5.00 Preservative appropriate amount fragrance appropriate amount Purified water Balance Total 100.00

Example 22 Packing agent

[0072]

(Table 25) (Components) (% by weight) Polyvinyl alcohol (Gohsenol EG-30, manufactured by Nippon Gohsei) 15.00 Carboxymethyl chitin aqueous solution (chitin liquid HV-10, manufactured by Ichimaru Falcos) 5.00 Triglucopolysaccharide ( 0.50 xanthan gum 0.50 sodium carboxymethylcellulose 0.50 titanium oxide 15.00 aluminum magnesium silicate 1.00 1-isostearoyl-3-myristoyl-glycerol 1.00 diglycerin 1. 50 Polyoxyethylene isocetyl ether (20EO) 1.00 Buttonpiex (50% water / ET) 10.00 Mulberry extract (dry solid) 0.10 Hop extract (dry solid) 0.10 Ethanol 5.00 Preservative Qs perfume qs purified water balance meter 100.00

Example 23 Emulsion

[0074]

(Ingredients) (% by weight) Palmitic acid 0.50 Olive oil 2.00 Cetanol 1.00 Jojoba oil 5.00 Monohexadecyl phosphate sodium salt 2.00 Sorbitan monostearate 0.50 Glycerin 15.00 Ethanol 5.00 Hibamata extract (30% water / ET) 5.00 Carrot extract (50% water / ET) 5.00 Vetch extract (50% water / ET) 1.00 Lactic acid 2.00 Purified water Balance meter 100. 00

Example 24 Emulsion

[0076]

(Ingredient) (% by weight) Arginine 0.40 Lysine 0.40 Cetyl alcohol 1.00 Glycerin 3.50 Lactic acid 0.20 Sodium lactate 0.30 Preservatives Appropriate amount Fragrance Appropriate amount Purified water Balance meter 100.00

Example 25 Poultice

[0078]

(Component) (% by weight) α-monoisostearyl glycer ether 1.00 pentyl glycol methyl polysiloxane neocaprate 4.00 glycerin 35.00 purified water 15.80 sodium polyacrylate 5.50 potassium aluminum sulfate 1.00 Polyacrylic acid aqueous solution (10%) 15.00 Light anhydrous silicic acid 2.00 Hibamata extract (water) 2.00 Carrot extract (50% water / ET) 2.00 Kiwi extract (50% water / ET) 1.00 Ginkgo extract (dry solid) 0.01 Bulberry extract (water) 0.50 Purified water Balance meter 100.00

[0079]

The collagen gel contraction promoter of the present invention is
It has an excellent skin thinning effect and skin tightening effect.

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁶ Identification code Agency reference number FI Technical display location A61K 35/78 A61K 35/78 QH NDC V ADA ADAB 35/80 35/80 Z 35 / 84 35/84 A (72) Inventor Yoshinori Takema 2606 Akabane, Kaigamachi, Haga-gun, Tochigi Pref.

Claims (3)

[Claims] 1. Ginkgo, Ginseng, Hibamata, chamomile, perilla, perilla, carrot, fennel, mulberry, gentian, burdock, shiitake mushroom, garlic, hop, buttonpi, yukinoshita, lettuce, bukuro, rosemary, milk vetch, kiwi, salvia and A collagen gel shrinkage promoter comprising one or more plants selected from Spiraea or an extract thereof as an active ingredient. 2. A composition for improving skin thickening, comprising the collagen gel contraction promoter according to claim 1. 3. A skin tightening composition comprising the collagen gel contraction promoter according to claim 1.