JP2007277121A - Keratinocyte contractile agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a keratinocyte contraction-accelerating agent excellent in safety. <P>SOLUTION: The keratinocyte contractile agent contains Phellodendron amurense Bark or an extract of the same as an active ingredient. The keratinocyte contractile agent can make the pores inconspicuous or improve the pores, since the agent strongly contract epidermis keratinocyte by acting on epidermic cells of the skin. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a keratinocyte contracting agent that acts on epidermal cells of the skin and contracts epidermal keratinocytes.

Many women take various skin care daily to make the pores less noticeable or improve to always look younger or healthier. As such a component, since the skin condition is likely to vary depending on individual differences and physical condition of the day, a highly safe component is desired for skin care.
As a component of a skin care agent for making pores less noticeable or improving, it is known to use a component having an action of contracting epidermal keratinocytes constituting the skin (Patent Document 1). Further, as a method for making pores inconspicuous, it is known to combine a keratinocyte contracting agent and a dermal tightening agent for the purpose of synergistically contracting the skin epidermis and dermis from both sides (Patent Document 2). . However, it was not known at all that yellowfin plant extracts cause keratinocyte contraction.
JP 2002-187817 A JP 2005-298403 A

  An object of the present invention is to provide a keratinocyte contraction agent having excellent safety.

  As a result of further searching for plant components that contract keratinocytes, the present inventors have found that an extract of the citrus family yellowfin has an excellent keratinocyte contracting action.

  That is, this invention provides the keratinocyte contraction agent which uses yellowfin or its extract as an active ingredient.

  The keratinocyte contracting agent of the present invention acts on the epidermal cells of the skin and strongly contracts the epidermal keratinocytes, so that the conspicuous pores can be made inconspicuous or improved.

  The yellowfin used in the present invention is Ephellodendron amurense Bark.

  The yellowfin in the present invention can be used in the above-ground parts such as leaves, stems, buds, flowers, woody parts, bark parts (bark), and underground parts such as roots and tubers, seeds, fruits, resins, etc. It is preferable to use a part (bark). Moreover, you may use the buckwheat currently distribute | circulated in the market.

  The yellowfin in the present invention can be used as a plant as it is or by squeezing it, a dried product obtained by drying the plant itself, or a pulverized product thereof, or an extract thereof. Is preferable, and an extract from a dried product or a pulverized product thereof is more preferable. Moreover, you may use a commercially available agaric extract for these extracts.

As an extract, various solvent extracts obtained by a known extraction method such as extracting yellowfin at room temperature or under heating or using an extraction device such as a Soxhlet extractor, diluted solutions thereof, and concentrated solutions thereof A liquid, its dry powder, or paste is mentioned.
Known extraction methods include, for example, immersion, decoction, leaching, reflux extraction, supercritical extraction, ultrasonic extraction, and microwave extraction.

  As the extraction solvent used for obtaining the extract, either a polar solvent or a nonpolar solvent can be used. For example, water; alcohols such as methanol, ethanol, propanol and butanol; polyhydric alcohols such as propylene glycol and butylene glycol; ketones such as acetone and methyl ethyl ketone; esters such as methyl acetate and ethyl acetate; tetrahydrofuran and diethyl ether Linear and cyclic ethers such as polyethylene; polyethers such as polyethylene glycol; halogenated hydrocarbons such as dichloromethane, chloroform and carbon tetrachloride; hydrocarbons such as hexane, cyclohexane and petroleum ether; aroma such as benzene and toluene Group hydrocarbons; pyridines and the like, and these can be used alone or as a mixture. Among these, it is preferable to use a polar solvent, and it is particularly preferable to use a water-alcohol solvent (40% to 100% (v / v)), and more preferably 80 to 100% (v / v). Here, the alcohol type refers to the above alcohols and / or polyhydric alcohols, among which alcohols are more preferable, methanol, ethanol, propanol and the like are more preferable, and ethanol is particularly preferable.

  Extraction from the plant material of the present invention can be performed, for example, as follows. That is, using 1 to 30 parts by mass of a solvent with respect to 1 part by mass of a plant, 0.5 to 30 days at 4 to 100 ° C, preferably 10 to 70 ° C, and 0.5 to 30 hours. It may be extracted for 1 day, especially 1 to 15 days.

The yellow extract is preferably used after removing inactive impurities by a known technique such as liquid-liquid distribution, solid-liquid distribution, filtration membrane, activated carbon, absorption resin, ion exchange resin and the like. As the solvent used at this time, those exemplified as the extraction solvent can be used. Moreover, you may use these, after giving processes, such as a deodorizing and a decoloring, by a well-known method as needed.
The yellow extract of the present invention may be a specific extract fraction obtained by combining the above solvent extractions. Specifically, for example, a hydrophobic fraction obtained by further extracting a water-ethanol mixed extract of yellowfin using a hydrophobic solvent such as hexane, diethyl ether, benzene and the like.

  As shown in Examples below, yellowfin extract acts on epidermal cells and contracts keratinocytes. Since the pores contract by contracting the epidermal keratinocytes, yellowfin or its extract can be used as cosmetics, quasi-drugs, pharmaceuticals, etc. to contract the pores and make the pores less noticeable or improved It can be used as a keratinocyte shrinking agent. In addition to the normal skin surface, the preparation may be used after depilation and further after removal of the stratum corneum.

The keratinocyte shrinking agent of the present invention is used in various forms such as a medicinal skin external preparation such as an ointment and a cosmetic skin external preparation, specifically, emulsified cosmetics, creams, emulsions, lotions, gels and the like. preferable. Such a preparation can be obtained by mixing and dispersing directly or together with a pharmaceutically acceptable carrier and then processing into a desired form by a general production method. In this case, in addition to the yellowfin extract of the present invention, oily bases such as vegetable oils and animal oils commonly used in such forms, analgesic anti-inflammatory agents, analgesics, bactericidal disinfectants, astringents, emollients, hormone agents , Vitamins, humectants, ultraviolet absorbers, alcohols, chelating agents, pH adjusters, preservatives, thickeners, pigments, fragrances, and the like can be appropriately blended within a range that does not interfere with the effects of the present invention.
Moreover, the said formulation of this invention is not limited to cosmetics, A pharmaceutical, a quasi-drug, pharmaceutical cosmetics, etc. are included.

  The blending amount of yellowfin or its extract of the above-mentioned preparation of the present invention is usually 0.0001 to 20% by mass, particularly preferably 0.001 to 5% by mass of the total composition as a dry product.

Hereinafter, the present invention will be described in more detail with reference to examples.
Production Example 1 Production of yellowfin extract 10 L of 95% (v / v) ethanol aqueous solution was added to 1.0 kg of yellowfin bark, extracted for 7 days at room temperature, and filtered to obtain an extract (evaporation residue: 1. 2 (w / v) This was concentrated under reduced pressure and further dried under reduced pressure to obtain a yellow extract.

Production Example 2 Production of hydrophobic fraction of yellowfin extract 10 kg of 95% (v / v) aqueous ethanol solution was added to 1.0 kg of yellowfin bark, extracted for 7 days at room temperature, and filtered to obtain an extract. The extract was concentrated under reduced pressure, further extracted with 0.6 L of hexane, and filtered to obtain an extract. This was concentrated under reduced pressure and further dried under reduced pressure to obtain a yellowfin extract hydrophobic fraction (evaporation residue: 1.0 (w / v)).

Example 1: Keratinocyte gel contracting action
Type I collagen (Cell Matrix Type-IA: Nitta Gelatin), MCDB153 medium (SIGMA: 5-fold concentration), 20 mM HEPES (DOJINDO) and purified water were mixed well with ice cooling, and then added to a 24-well plate (Falcon). 500 μL of each well was injected and heated to 37 ° C. in an incubator for gelation. Keratinocytes (HEKn: Cascade Biologics) are seeded at 2 × 10 ⁴ cells / cm ² at 1 × L using the keratinocyte culture medium (Epi Life: Kurabo) on the obtained collagen gel, cultured for 24 hours, and then scrapered. The collagen gel was peeled from the culture dish. Immediately thereafter, yellowfin extract and yellowfin hydrophobic fraction were added at final concentrations (solids concentration) of 0.001% and 0.01%. One hour after the addition, the state of contraction was photographed using a Minolta α707-si camera and a 50 macro lens. After photo development, the shrink ring was copied onto OHP sheet paper, and the area inside the shrink ring was determined using image analysis software Image-Pro PLUS (Media Cybanetics).
Control (only gel peeling) The shrinkage rate (%) (gel area ratio) when the area of the gel was taken as 100% was determined. The results are shown in the table.

  From this result, it was found that the yellowfin extract has an excellent keratinocyte contractile activity and a higher keratinocyte contractile activity in the hydrophobic fraction.

Claims (1)

  A keratinocyte contractor containing yellowfin or its extract as an active ingredient.