KR20170004626A - A cosmetic composition using silkworm-derived extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition using a silkworm-derived extract. More specifically, the present invention relates to a cosmetic composition using a silkworm-derived extract, containing a composite mixture obtained by mixing the silkworm-derived extract, a bee venom extract, and a plant extract at the ratio of 1-5:1-5:1-10. According to the present invention, the cosmetic composition contains, as main ingredients, the silkworm-derived extract showing efficacy in wrinkle amelioration and skin whitening, in addition to the bee venom extract which has antioxidant ability. By further containing the plant extract as a natural extract, the cosmetic composition exhibits antioxidative and skin-whitening functions, and also improves skin immunity.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition using a silkworm-derived extract,

The present invention relates to a cosmetic composition using silkworm-derived extract.

Silk protein has been shown to be effective against weight loss and body fat (insomnia, insomnia) and insomnia, while silkworms such as caterpillar, pupa, silkworm, , Antioxidant activity, brain function activity, diabetes, blood pressure and antitumor effect. These silkworms are very sensitive to the cleanliness and pesticides at the time of cultivation, unlike other natural products, and they are already known as natural pollution-free products since they are mainly used as experimental insects for stability test. Thus, silkworms and by-products are currently used as the main ingredients of cosmetics by hydrolyzing sericin and fibroin proteins, which have effects of skin coating effect, moisturizing, antioxidant ability and skin affinity. As a related prior art, Korea Patent No. 10-0494229 (registered on May 31, 2005, name: Sericin manufacturing method and mask pack using the same) and Korea Patent No. 10-0542266 (registered on Jan. 2006 2006 March 03, name: cosmetic pack composition containing sericin protein extracted from silkworm cocoon).

On the other hand, as interest in natural antimicrobial substances has increased recently, many studies have been conducted. Bee venom, which is a pure natural substance and strong antimicrobial and antiinflammatory effect, has been used for a long time ago in diseases such as arthritis and gout due to a side effect and a risk of residual side effects. The bee venom of domestic honey bee (Apis mellifera) can be obtained by collecting only a large amount of pure bee venom without damaging the honey bee by using the bee venom collecting device using the electric impact method, and then removing the foreign matter and freeze drying the purified bee venom. These bee venom is composed of various components in a complex composition, and melittin, which is a main ingredient, plays an anti-inflammatory and antimicrobial action, strong analgesic action and immunity enhancement. Bee venom has both antibacterial activity against gram negative and positive bacteria, but has stronger effect against gram positive bacteria. Bee venom also promotes the regeneration of skin damaged by ultraviolet light.

As related prior art, Korea Patent Publication No. 2010-0118629 entitled " Cosmetic composition containing bee venom or bee venom extract "includes " cosmetic composition containing bee venom or bee venom extract having an effect of skin antioxidation, anti- ≪ / RTI > Korean Patent Laid-Open Publication No. 2014-0067333 entitled " Anti-aging cosmetic composition containing lipid-soluble fraction of bee venom and method for producing the same "discloses a liposoluble fraction of bee venom divided by a fat-soluble solvent and a water- : In a volume ratio of 0.01 to 0.5.

In addition, the skin is always in direct contact with the external environment of the body, protects the living body from various stimuli or environment, and plays an active role in generation and destruction of new cells compared to other organs. In addition, it protects the body from physical abrasion, protects the inside of the body from moisture loss, protects it from ultraviolet rays generated from the sun, and plays a very important role in controlling body temperature. Such skin causes pollution, dryness, trouble and skin immune system abnormality due to various physical factors, external environment factors, infection and the like, resulting in dermatitis, allergy and worsening. In addition, the skin often has keratin due to drying when the moisture content of the skin decreases. Also, the skin's luster disappears, fine wrinkles easily occur, and skin aging begins early. That is, in order to have improved skin, it is essential to maintain skin moisture.

Accordingly, the inventors of the present invention have focused on the above-mentioned technology, and have found that a bee venom extract having antioxidative ability as a main ingredient while containing a silkworm-derived extract as a main ingredient and a plant extract as a natural extract are further included to exhibit antioxidative and whitening functions, Thereby preparing a cosmetic composition capable of enhancing immunity, and the present invention has been completed.

Accordingly, it is a technical object of the present invention to provide a cosmetic composition using the silkworm-derived extract.

According to an aspect of the present invention,

A silkworm extract, a silkworm extract, a silkworm extract, a silkworm extract, a silkworm extract, a silkworm extract and a plant extract in a ratio of 1: 5: 1 to 5: 1 to 10.

In the present invention, the silkworm-derived extract is characterized by being an extract of at least one silkworm-derived substance selected from the larvae, pupa, silkworm and silk of silkworm.

In addition, the plant extract according to the present invention is a mixed plant extract obtained by mixing a willow extract, a whitetoptera extract, and a centipede extract in a ratio of 1: 2: 1 to 2: 1 to 2.

In the present invention, the composite is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition.

Further, in the present invention,

1 to 10% by weight of at least one herbal essential oil selected from lavender essential oil, jasmine essential oil, rosemary essential oil, chamomile essential oil, position hazel essential oil and ylang ylang essential oil; 1 to 10% by weight of at least one moisturizing oil selected from olive oil, argan oil, avocado oil, jojoba oil, camellia oil, grape seed oil and evening primrose oil; 1 to 10% by weight of at least one moisturizing agent selected from glycerin, honey and aloe vera gel; And 5 to 10% by weight of an emulsifier selected from olive oil wax and shea butter.

In the present invention, the composition may further comprise at least one additive selected from vitamin E, alginic acid, and grapefruit seed extract.

In the present invention, the cosmetic composition may be formulated into a lotion, essence, lotion, cream, pack, gel, ointment, patch, spray, mask sheet and detergent.

The cosmetic composition of the present invention contains as an essential ingredient a bee venom extract having antioxidative ability as a main ingredient and an extract from silkworm having an effect of improving wrinkles and skin whitening, and further contains a plant extract as a natural extract, It has an effect of enhancing skin immunity while exhibiting functionality.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition using a silkworm-derived extract, more specifically, a silkworm-derived extract, a bee venom extract, and a plant extract in a ratio of 1: 5: 1 to 5: 1 to 10 To a cosmetic composition using the silkworm-derived extract.

In particular, the cosmetic composition of the present invention contains fibroin, sericin protein and the like by including an extract derived from the silkworm, thereby enhancing the skin barrier and imparting moisturizing power, as well as exhibiting wrinkle improvement, whitening and antioxidative function .

Preferably, the silkworm-derived extract may be an extract of one or more silkworm-derived substances selected from silkworm larvae, pupa, silkworm and silkworm. At this time, the extraction solvent for extracting the active ingredient from the silkworm-derived material is not limited thereto, but may be selected from water, an organic solvent, or a mixture thereof. The organic solvent may be a non-polar solvent such as ether, chloroform, benzene, hexane, dichloromethane or the like or a mixture thereof for a polar solvent such as an alcohol having 1 to 5 carbon atoms, ethyl acetate or acetone. For example, the alcohol having 1 to 5 carbon atoms may be selected from methanol, ethanol, propanol, butanol, isopropanol, 1,3-butylene glycol and the like.

The extract of the silkworm-derived material includes fractions obtained by further fractionating the primary extract extracted using the extraction solvent with an extraction solvent having a different polarity. For example, it may be a fraction obtained by extracting an extract of a golden silkworm-derived substance with an alcohol having 1 to 5 carbon atoms and then fractionating again with a solvent having a different polarity such as ether, benzene or hexane. Two or more kinds of solvents may be used for the fractionation, and each solvent extract may be used sequentially or in combination according to the polarity of the solvent, but not limited thereto.

In the cosmetic composition of the present invention, the effective ingredient through hydrolysis in the silkworm-derived material may further include a hydrolyzate using acid hydrolysis and enzymatic hydrolysis. At this time, the hydrolyzate obtained through acid hydrolysis can be obtained by decomposing the silkworm-derived material using one or a mixture of two or more selected from the group consisting of hydrochloric acid, phosphoric acid and sulfuric acid.

The extract or the hydrolyzate obtained by performing the fractionation process may be filtered, concentrated or dried to remove the solvent, and may be subjected to both filtration, concentration and drying. Specifically, the filtration can be performed using a filter paper or a vacuum filter, and the concentration can be reduced by using a reduced pressure concentrator, for example, a rotary evaporator. The drying can be performed by, for example, freeze drying. The larvae, pupa, silkworm and silk obtained from the silkworm of the present invention can be produced as a raw material or in a powder state by pulverization.

In addition, the cosmetic composition of the present invention contains the bee venom extract as a main ingredient of the active ingredient, while containing the silkworm extract as a main ingredient of the effective ingredient.

At this time, the bee venom refers to a clear and transparent liquid stored in the money of the queen bee or the worker bee. The peptides contained in this beak contain about 40 components that have antibacterial and anti-inflammatory properties. Representative peptides are melittin, which accounts for more than 50% of bee venom. Melitin is a representative antibacterial substance of bee venom, which is an active protein with 26 amino acids. It has an affinity for mixing both water and oil, and acts to change the membrane permeability to other cell membranes. Melitin has anti-bacterial and anti-inflammatory action, so it is good for acne skin and it has skin regeneration effect.

Accordingly, the cosmetic composition of the present invention exhibits excellent antimicrobial and anti-inflammatory action as it contains the bee venom extract and can help skin regeneration. In addition, the inclusion of the bee venom extract improves pigmentation caused by ultraviolet light to exhibit a skin whitening effect, induces the differentiation of keratinocytes, and is an important source of natural moisturizing factors possessed by keratinocytes And promotes the expression of filaggrin, thereby showing a skin moisturizing effect.

At this time, the bee venom extract can be obtained by extracting bee venom according to a conventional method in the art. For example, bee venom extract can be obtained by extracting bee venom into water, an organic solvent or a mixed solvent of water and an organic solvent, and purifying and separating the solvent. The organic solvent is not particularly limited and may be C1-C5 lower alcohol, ether, ethyl acetate or chloroform. The C1 to C5 lower alcohol may be any one or a mixture of two or more selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol. In this case, the volume of the organic solvent in the mixed solvent of water and organic solvent may be 10 to 90% (v / v). Specifically, the bee venom extract is mixed with a mixed solvent containing 10 to 90% (v / v) ethanol, more specifically 70% (v / v) ethanol, based on the total volume of the mixed solvent May be an extracted extract.

Further, the cosmetic composition of the present invention is characterized by containing the plant extract as a main ingredient of an effective ingredient. By including plant extracts, which are natural extracts, it is possible to reduce the irritation of the skin and give more functionality according to the plants derived.

Preferably, the plant extract may be a mixed plant extract obtained by mixing Willow, Extract, and Centella asiatica at a ratio of 1: 2: 1 to 2: 1 to 2. More preferably in a ratio of 1: 1: 1.

At this time, the willow extract has antioxidant, antimicrobial action, and contains salicylic acid (BHA) ingredient to help alleviate inflammation, and willow extract is a natural extract, so it is more irritating than salicylic acid. The above-mentioned Hwasungcho extract is the powder obtained from the outpouring of the capsicum, and has antibacterial, antifungal, antiviral, antioxidant and anti-inflammatory effects and a unique flavor due to the essential oil component contained therein. In addition, the centilla extract is an effective ingredient for skin regeneration, and is a component capable of minimizing scarring by synthesizing collagen. Accordingly, the cosmetic composition of the present invention can help antioxidant, antibacterial and skin regeneration by containing the above-mentioned mixed plant extract.

These plant extracts can be obtained by physically pulverizing dried plants and then extracting them using a suitable solvent such as water, methanol, ethanol, butanol, chloroform, dichloromethane, hexane, ethyl acetate or the like, .

In addition, the cosmetic composition of the present invention can increase the effect of skin moisturizing, antioxidant and whitening effects by synergistic action by mixing the silkworm-derived extract, bee venom extract and plant extract at a specific ratio, Silkworm extract, bee venom extract, and plant extract are mixed in a ratio of 1: 5: 1 to 5: 1 to 10. When mixed at the above ratio, it is possible to maximize the effect of enhancing the skin barrier and moisturizing while exhibiting wrinkle improvement, skin whitening and antioxidative effect by the silkworm extract and bee venom extract. More preferably, the composite is prepared by mixing in a ratio of 1: 1: 3 to 6.

Also, the above-mentioned composite is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition.

In the cosmetic composition of the present invention, preferably 1 to 10% by weight of at least one herbal essential oil selected from the group consisting of lavender essential oil, jasmine essential oil, rosemary essential oil, chamomile essential oil, position hazel essential oil and ylang ylang essential oil; 1 to 10% by weight of at least one moisturizing oil selected from olive oil, argan oil, avocado oil, jojoba oil, camellia oil, grape seed oil and evening primrose oil; 1 to 10% by weight of at least one moisturizing agent selected from glycerin, honey and aloe vera gel; And 5 to 10% by weight of an emulsifier selected from olive oil wax and shea butter.

In the cosmetic composition of the present invention, the lavender essential oil of the herbal essential oil is effective for skin cleansing and wounds treatment, and jasmine essential oil is effective for all skin and is effective for calming and restoring dry and damaged skin and sensitive skin, Rosemary essential oil is known to be effective when there is skin trouble such as facial or body rash or itching. In addition, chamomile essential oil has anti-allergy, skin soothing, anti-inflammation and anti-cancer effects, and position hazel essential oil and ylang ylang essential oil provide nutrient and moisture to rough skin and dry skin, , It is good for dry skin and is known to have a soothing effect on the skin. Therefore, the cosmetic composition of the present invention contains the herbal essential oil, thereby controlling the viscosity of the composition and improving the effect of soothing, anti-inflammation, moisturizing, etc. of the essential oil of the herb, thereby improving the relieving and improving effect of the atopic symptoms. At this time, if the herbal essential oil is contained in an amount of less than 1% by weight, the effect of the essential oil of the herb may not be exhibited. If it exceeds 5% by weight, the viscosity of the herbal essential oil may be lowered. By weight based on the total weight of the composition.

In addition, in the cosmetic composition of the present invention, the moisturizing oil is a substance that imparts viscous and oily properties to the cosmetic composition. It enhances the moisturizing power of the cosmetic composition and protects the skin by forming an oil film on the skin, do. In particular, the olive oil provides excellent moisture resistance, and it contains vitamin A and vitamin E, so it has excellent antioxidative effect, so that it calms the skin and maintains a healthy and healthy skin. In addition, the argan oil is effective for preventing aging / wrinkles, atopic skin, skin elasticity, and skin inflammation. The avocado oil has an effect of skin moisturizing and skin regeneration. Jojoba oil promotes the spreading and lubrication of the skin, It absorbs quickly through the skin. It contains a complex of minerals and vitamins that softens the skin and is effective in moisturizing and cleansing. Camellia oil, grape seed oil, and evening primrose oil also protect the skin's lipid layer against atopic dermatitis patients. If the amount of the moisturizing oil is less than 1% by weight, the viscosity and oiliness are insufficient to exhibit the effect. If the amount of the moisturizing oil is more than 10% by weight, the viscosity is lowered, Preferably in the range of 1 to 10% by weight.

In the cosmetic composition of the present invention, the moisturizing agent is a substance that prevents evaporation of oil / water in the skin and improves the moisturizing power. The moisturizing agent is used in an amount of 1 to 10% by weight As shown in Fig.

Also, in the cosmetic composition of the present invention, the emulsifier is used as a thickening agent of the cosmetic composition. If the emulsifier is less than 5% by weight, the effect is insufficient and is not preferable. If it exceeds 10% by weight, There is a fear that it is lowered.

As described above, the cosmetic composition of the present invention contains herb essential oil, moisturizing oil, moisturizing agent and emulsifier while containing silkworm extract, bee venom extract and plant extract as an active ingredient, thereby maintaining skin oil / And protects the skin by forming a protective film on the skin, and has appropriate physical properties such as durability of the cosmetic composition and is harmonized with the natural extract, thereby exhibiting effects such as wrinkle improvement, skin whitening, antioxidant ability as a whole Preference, moisturizing power and the like can be improved.

The cosmetic composition of the present invention may further comprise at least one additive selected from vitamin E, alginic acid and grapefruit seed extract. Such an additive acts as a preservative of the cosmetic composition, more preferably 1 to 5% by weight based on the total weight of the cosmetic composition. When the content is less than 1% by weight, the effect is insignificant. When the content is more than 5% by weight, the preservation effect is not remarkable, which is not preferable.

In addition, the cosmetic composition according to the present invention can be formulated into lotion, essence, lotion, cream, pack, gel, ointment, patch, spray, mask sheet and detergent. At this time, it is possible to further include a cosmetically acceptable additive.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to examples, comparative examples and test examples. However, these examples, comparative examples and test examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited thereto.

<Example 1> Preparation of silkworm-derived extract from silkworm-derived material

1,3-butylene glycol as an extraction solvent was added in an amount of 10 times as much as 0.1 kg of a mixture of cocoon pulverized material, dry third-instar larvae powder and dry pupa powder mixed at a ratio of 1: 1: 1. The extraction was carried out three times with a hot water bath at 90 캜 for about 30 minutes. After filtration, the mixture was cooled at room temperature, stored at 4 ° C for 1 day, and concentrated by filtration.

Example 2: Preparation of bee venom and preparation of bee venom extract

Bee venom was obtained by electric shock method. The bee venom obtained from the electric shock method was poured with distilled water to float bees or debris in bee venom. Filtered suspended bees or debris to obtain purified beacon.

The obtained bee venom was extracted with 70% ethanol. Specifically, 70% ethanol corresponding to 3 times the volume of the bee venom obtained was added, and the mixture was extracted and degreased with stirring at room temperature five times. 70% ethanol was added to the degreased bees poison four times, and the mixture was refluxed three times and then immersed at 15 ° C for 2 days. The precipitate was filtered and centrifuged to separate the residue and filtrate, and the separated filtrate was concentrated under reduced pressure to obtain an extract. After the resulting extract was suspended in water, the pigment was removed using ether, and the aqueous layer was extracted with butanol five times. The obtained organic solvent layer was concentrated under reduced pressure to give an extract. The resulting extract was dissolved in methanol, ethyl acetate was added, and the resulting precipitate was dried to obtain a bee venom extract.

Example 3: Preparation of plant extracts

The seeds were washed thoroughly in running water and cut into small pieces. Distilled water was added to the seeds of the seeds, and the seeds were milled. The seeds were heated at 100 ° C for 10 minutes and filtered through gauze to prepare Peppermint extract.

In the same manner as in the above-mentioned method, Hwasungcho extract and Centella asiatica extract were prepared.

The mixed plant extracts were prepared by mixing the willow, extract, and centipede extracts at a ratio of 1: 1: 1.

< Manufacturing example  1 to 6> Cosmetics  Preparation of composition

The silkworm extract, bee venom extract and mixed plant extract prepared in the above Example were prepared, the temperature of the extract was adjusted to 60 ° C, the emulsifier was heated at 70 ° C to obtain a solution of emulsifier, , Herbal essential oil, moisturizing oil, and moisturizer were mixed and stirred at 70 캜. Thereafter, the mixture was gradually cooled to prepare a lotion-type cosmetic composition. Olive oil wax was used as an emulsifying agent used in the preparation. Ylang Ylang essential oil was used as a herbal essential oil, olive oil was used as a moisturizing oil, and glycerin was used as a moisturizing agent.

The composition according to each example is shown in Table 1 below.

Production Example 1 Production Example 2 Production Example 3 Production Example 4 Production Example 5 Silkworm-derived extract 10g 7.5g 7.5g - 15g Bee venom extract 10g 15g 7.5g 15g - Mixed plant extract 60g 57.5g 65g 65g 65g

<Test Example> Sensory Evaluation on Cosmetic Composition

The sensory evaluation of the cosmetic compositions prepared in Preparation Examples 1 to 5 was carried out. The cosmetic composition prepared in Preparation Examples 1 to 5 and the commercially available anti-aging lotion A (manufacturer: 00) and the whitening lotion B (manufacturer: 00) were used as Comparative Examples 1 and 2, Respectively.

At this time, the subject was evaluated by subjecting 21 adult women (three per each cosmetic composition) having no skin allergy symptoms of 20 years old or more to the skin at least twice a day for at least one week.

The scores were in the range of 1 to 10, and the best score was 10 points. The results are shown in Table 2 below. (1: bad, 5: normal, 10: very good)

Production Example 1 Production Example 2 Production Example 3 Production Example 4 Production Example 5 Comparative Example 1 Comparative Example 2 Skin moisturizing 9.4 9.2 9.2 7.9 8.2 9.2 9.0 wrinkle improvement 9.4 9.4 9.4 8.2 8.0 8.4 8.8 Skin whitening 9.6 9.4 9.4 8.0 8.0 8.2 8.4 Synthesis 9.6 9.6 9.5 8.1 8.0 8.0 8.2

As shown in Table 2 above, the sensory evaluation results showed that the cosmetic composition of the present invention exhibited more excellent effects than the case of Comparative Examples 1 and 2, in which the cosmetic composition of the present invention was marketed as a conventional whitening lotion. Particularly, in Production Example 1, there was an evaluation that the effect of remarkable whitening and wrinkle improvement was shown.

It was evaluated that the cosmetic composition of the present invention exhibits excellent effects because it has a longer maintenance period of moisturizing power than the commercial product.

In the case of Production Example 4 which did not contain the silkworm-derived extract, however, it had lower scores in overall skin moisturizing, wrinkle improvement, skin whitening and the like compared with the case of Production Examples 1 to 3, 5, the evaluation score was evaluated to be low overall as in Production Example 4. [

From the results of the above Table 2, it can be confirmed that the cosmetic composition of the present invention contains all the silkworm extract and bee venom extract and contains the mixed plant extract, thus showing excellent effects on skin moisturization, wrinkle improvement and skin whitening . This is because the inclusion of a natural product extract having excellent wrinkle and skin whitening effect as an active ingredient reduces the amount of the chemical composition to improve the moisturizing power while reducing the skin irritation force and maximizing the effect of wrinkles and whitening Effect.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention as defined by the following claims. It can be understood that it is possible.

Claims (7)

Wherein the silkworm extract, the silkworm extract, the silkworm extract, the bee venom extract and the plant extract are mixed at a ratio of 1: 5: 1 to 5: 1 to 10. The method according to claim 1,
Wherein the silkworm-derived extract is an extract of at least one silkworm-derived substance selected from the group consisting of larvae, pupa, silkworm and silk of silkworm. The method according to claim 1,
Wherein the plant extract is a mixed plant extract obtained by mixing willow, extract, and centipede extracts at a ratio of 1: 2: 1 to 2: 1: 2. The method according to claim 1,
Wherein the composite is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition. The method according to claim 1,
Based on the total weight of the composition,
1 to 10% by weight of at least one herbal essential oil selected from lavender essential oil, jasmine essential oil, rosemary essential oil, chamomile essential oil, position hazel essential oil and ylang ylang essential oil,
1 to 10% by weight of at least one moisturizing oil selected from olive oil, argan oil, avocado oil, jojoba oil, camellia oil, grapeseed oil and evening primrose oil,
1 to 10% by weight of at least one moisturizing agent selected from glycerin, honey and aloe vera gel and
Further comprising 5 to 10% by weight of an emulsifier selected from the group consisting of olive oil wax and shea butter. The method according to claim 1,
Wherein the composition further comprises at least one additive selected from vitamin E, alginic acid and grapefruit seed extract. The method according to claim 1,
Wherein the cosmetic composition is formulated with a lotion, essence, lotion, cream, pack, gel, ointment, patch, spray, mask sheet and detergent.