KR20190068363A - Cosmetic composition for skin Humectant comprising Opuntia Ficus-Indica fruit extract and mixed compound of panthenol, hyaluronic acid and betaine - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin moisturization containing Opuntia ficus-indica fruit extracts and a composite compound of panthenol, hyaluronic acid and betaine as active ingredients. The composition comprises Opuntia ficus-indica fruit extracts, panthenol, hyaluronic acids and betaine by a specific weight ratio, and may greatly increase a moisturizing change rate of skin compared when only single ingredient is used. In addition, the composition has no cytotoxicity and skin side effect, thereby being safely used for a long time especially, as a cosmetic composition.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for skin moisturizing, comprising a compound of pectin, hyaluronic acid and betaine as an active ingredient, and a cosmetic composition for skin moisturizing,

The present invention relates to a cosmetic composition for skin moisturizing, and more particularly to a cosmetic composition for skin moisturizing, which comprises a combination of a fruit extract of Opuntia ficus-Indica with panthenol, hyaluronic acid and betaine, To provide a sufficient amount of moisture to the skin, and to a cosmetic composition for skin moisturization which is safe when applied to a human body.

The structure of the skin is divided into three layers of epidermis, dermis and subcutaneous fat tissue in order from the outside, and functions to protect the human body from chemical and physical stimulation by the external environment, and is the largest among all the organs in the body It is an institution. When viewed from the side two times heavier than the weight reaches the brain 3kg, expanding the skin, wide and occupies an area of approximately 18m ^2. One square inch (6.5 square centimeters) of skin has 65 hair follicles, 100 oil spots, 650 sweat glands, 1,500 different types of neurons, and numerous nerves.

In particular, skin carries about 65 ~ 70% of the human body's moisture, that is, various physiologically active substances necessary for the human body, and helps the skin to keep its soft and moist state. It plays a role. The skin regulates body temperature by preventing the evaporation of water, not only prevents harmful bacteria from entering the body, but also kills invading bacteria.

The epidermis of the skin is divided into the stratum corneum, the granular layer, the superficial layer, and the basal layer from the outside. Cells of the stratum corneum act like bricks, and the intercellular lipids between the keratinocytes act like a mortar, (J. Invest. Dermatol. 80 (Suppl.), 44-49, 1983). The stratum corneum of the epidermis contains about 10 to 20% of water and exists in the outermost part of the human body, thereby inhibiting moisture evaporation to the outside of the body, while blocking excessive material penetration from the outside. The stratum corneum is a non-nucleated cell, flat grayish white pieces arranged in a lamellar structure (brick structure), intercellular lipids, natural moisturizing factor (NMF) Hold it so that it does not fall. In other words, it can be considered that the stratum corneum that fills the water is wrapped with a natural protective film that water does not pass through. The cells that constitute the stratum corneum have a high concentration of natural moisturizing factor (NMF), which is a water-soluble component, to help maintain proper moisture, and to provide skin flexibility. For example, In addition, it effectively combines with moisture to inhibit the drying of water from the skin (J. Invest. Dermatol. 54, 24-31, 1970).

However, there are various causes such as an artificial temperature control of the cooling / heating according to the change of the environment or the life pattern, frequent cleansing according to make-up habit, skin stress due to various stress and environmental pollution, The moisture of the stratum corneum is decreased, the skin becomes dry, the surface becomes rough, and the skin appears to be dull and dull. Therefore, the need for a skin moisturizer is increasing.

Conventionally, a humectant which absorbs water as a moisturizing agent or an occlusive moisturizer which prevents moisture evaporation is used, and as a result, a method of increasing water retention in the stratum corneum It was widely used. Most of the hugh martens are polyols such as glycerin, propylene glycol, 1,3-butylene glycol, and polyethylene glycol. However, they have a disadvantage in that they are hardened or sticky due to inherent viscosity and physical properties of the polyol when applied to the skin. As a closed humectant, essential fatty acids and lipid complexes and lipid components such as ceramides have been used (J. Invest. Dermatol. (5), 731-740, 1994), but disturbing the stability of the emulsified interfacial membrane structure in the emulsified formulations But it has drawbacks and is unsuitable for producing transparent gel-like products. In addition, the formulations containing the lamellar liquid crystals, which have been undergoing much research until recently, are known to have excellent moisture retention due to their similar merits to the keratinous structures of the skin, but they can be manufactured only in a specific composition of the raw materials and thus have problems in universal use.

Accordingly, the present inventors have completed the present invention by developing a composition that is harmless to the human body, has no skin irritation, and has excellent skin moisturizing effect that can give sufficient moisture to the skin.

One technical problem to be solved in the present invention is to provide a skin moisturizing composition comprising an extract of fruit of Opuntia ficus-Indica and a complex compound of panthenol, hyaluronic acid and betaine as an active ingredient And to provide a cosmetic composition for skin.

In one embodiment, the present invention relates to a skin moisturizing composition characterized by containing an extract of fruit of Opuntia ficus-Indica and a complex compound of panthenol, hyaluronic acid and betaine phosphorus as an active ingredient The present invention provides a cosmetic composition.

In the present invention, the above-mentioned " Opuntia Ficus-Indica " is one of the cacti of Opuntia , and is a native plant native to Jeju and South Seas of Korea. It is called " Opuntia ficus-Indica " It is widely known. The fruit of sordid cactus is red and has an acidic taste. It is rich in nutrients such as calcium, magnesium, sodium, iron and minerals and anti-aging flavonoids. Vitamin C content is 5 times that of aloe And phenolic compounds with antioxidant and anti-cancer effects. The content of dietary fiber is 30%, which is much higher than that of ordinary fruits and fresh vegetables. The content of magnesium is high, while that of sodium, potassium and phosphorus is comparable to those of other fruits. Especially, free amino acid content is high It is known that the content of serine, γ-aminobutyric acid, glutamine, proline, arginine and histidine is higher than other fruits. have.

In the present invention, the "Panthenol" is a provitamin of pantothenic acid (vitamin B5). That is, it is a substance that changes into vitamin B5 in the body. When panthenol enters the skin cells, it is commonly called vitamin B5 because it changes to pantothenic acid (vitamin B5).

In the present invention, the term "hyaluronic acid" refers to hyaluronic acid, hyaluronan, or hyaluronate, and is a type of polysaccharide called glycosaminoglycan. It is a substance attached to collagen and elastin that forms cartilage and promotes the supply of joint capsule fluid (lubricating oil) which softens joints. It is mainly found in the dermis of the skin and forms an important part of the skin tissue.

In the present invention, the above-mentioned "betaine" is an amino acid derived from sugar beet. It serves as a protective film for protecting the skin tissue by preventing penetration of harmful external substances such as ultraviolet rays, infrared rays, do.

In the present invention, "sausage cactus fruit extract" refers to an extract extracted from fruit of sausage cactus.

In addition, the above-mentioned "sausage cactus fruit extract" refers to any extract, fraction, purified product, diluted solution, concentrate, or dried product obtained from each step of extracting, fractionating, or refining All mixed forms.

The above-mentioned "sausage cactus fruit extract" can be obtained by using an extraction method and an extraction solvent known in the art. The extraction solvent may be selected from the group consisting of water, methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide, dimethylsulfoxide (DMSO) , 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof. The extract can be extracted at room temperature or warmed under the condition that the active ingredient of the sake extract of cactus fruit is not destroyed or minimized. Depending on the extraction solvent to be extracted, the degree of extraction and the degree of loss of the active ingredient may differ. Therefore, an appropriate extraction solvent should be selected and used. The extraction method is not particularly limited, and for example, cold extraction, ultrasonic extraction, reflux cooling extraction and the like can be used.

In addition, the above-mentioned "boiled cactus fruit extract" can be obtained by a conventional purification process in addition to the extraction method using the above-mentioned extraction solvent. For example, various purification processes have been carried out, including ultrafiltration with a constant molecular weight cut-off value, freezing filtration, centrifugation, various chromatography (size, charge, hydrophobicity or affinity chromatographic separation) But the present invention is not limited thereto.

In one specific embodiment of the present invention, the above-mentioned "sausage cactus fruit extract" can be obtained by shredding boiled cactus fruit at 20 to 35 DEG C, pulverizing it and immersing it in ethanol to ultrasonically extract it.

In the present invention, the "complex compound" is prepared by mixing panthenol, hyaluronic acid and betaine in an appropriate ratio. Preferably, panthenol, hyaluronic acid and betaine are mixed in a weight ratio of 0.4 to 0.6: 0.9 to 1.1: 1.4 to 1.6. More preferably, panthenol, hyaluronic acid and betaine are mixed at a weight ratio of 0.45 to 0.55: 0.95 to 1.05: 1.45 to 1.65, most preferably panthenol, hyaluronic acid and betaine are mixed at a weight ratio of 0.5: 1.0: 1.52 do. When the above ratio is exceeded, the skin moisturizing cosmetic composition according to the present invention does not exhibit remarkable skin moisturizing effect as compared with the above-mentioned single component.

In the present invention, the "cosmetic composition for skin moisturizing" is prepared by mixing a fruit extract of Opuntia ficus-Indica and the complex compound in an appropriate ratio. Preferably, the compound of the present invention is mixed at a weight ratio of 1.4 to 1.6: 2.7 to 3.3, more preferably the compound of the present invention is mixed at a weight ratio of 1.45 to 1.55: 2.8 to 3.2, Are mixed at a weight ratio of 1.5: 2.9 to 3.1. When the above ratio is exceeded, the skin moisturizing cosmetic composition according to the present invention does not exhibit remarkable skin moisturizing effect as compared with the above-mentioned single component.

Hereinafter, the cosmetic composition for skin moisturizing according to the present invention will be referred to as MultiEx SuperMoist.

In the present invention, the term "skin moisturizing" is intended to prevent the moisture of the skin from evaporating out of the body and prevent excessive penetration of foreign substances, thereby maintaining the moisture of the skin horny layer and preventing the skin from becoming dry or surface roughened, And to keep the skin soft and moist.

The cosmetic composition for moisturizing the skin of the present invention contains the active ingredient in an amount of 0.0001 to 20% by weight, preferably 0.001 to 15% by weight, more preferably 0.1 to 10% by weight, based on the total weight of the cosmetic composition do. When the amount is less than 0.0001 wt%, the skin moisturizing effect is weak. When the amount is less than 20 wt%, the increase in the effect of the skin is insignificant due to an insufficient increase in the content.

The cosmetic composition for moisturizing the skin may further contain ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients. For example, conventional auxiliaries and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and antioxidants.

The cosmetic composition for moisturizing the skin may be prepared in any formulations conventionally produced in the art. For example, be formulated as a solution, emulsion, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation or spray have. More particularly, the present invention relates to a cosmetic composition for oral use, which comprises nutritional cream, convergent lotion, supple lotion, pack, lotion, essence, nutritional gel, foundation, lipstick, makeup base, essence, cleansing foam, cleansing water, cleansing cream, spray, , Rinses, treatments, massage creams or other cleansers, and the like. However, the present invention is not limited thereto.

When the formulation of the cosmetic composition for skin moisturizing is a paste, cream or gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc or zinc oxide Etc. may be used.

When the formulation of the skin moisturizing cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, Propellants such as hydrocarbons, propane / butane or dimethyl ether.

When the formulation of the skin moisturizing cosmetic composition is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component. For example, fatty acid esters of water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used have.

When the formulation of the skin moisturizing cosmetic composition is in the form of a suspension, the carrier component may be a suspending agent such as water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition for skin moisturizing is an interface-active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyltaurate, Fatty acid amide ether sulfate, alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

The cosmetic composition for skin moisturizing of the present invention exhibits excellent skin moisturizing effect without cytotoxicity.

As described above, the cosmetic composition for skin moisturizing comprising the extract of Opuntia ficus-Indica according to the present invention and a complex compound of panthenol, hyaluronic acid and betaine as an active ingredient, The moisturizing amount of the skin can be remarkably increased as compared with the case where only the single component is used. In addition, there is no cytotoxicity and skin side effects, so that it can be safely used even when used for a long period of toxicity, and can be safely used particularly as a cosmetic composition and a pharmaceutical composition.

FIG. 1 is a graph showing changes in the amount of moisturizing change of composite compounds prepared by varying the composition ratio of panthenol, hyaluronic acid, and betaine.
FIG. 2 is a graph showing changes in moisturizing amount of cosmetic compositions for skin moisturization, which were prepared by varying composition ratios of selenium extract, panthenol, hyaluronic acid and betaine.
FIG. 3 is a graph showing the effect of improving the water loss (TEWL) of the external preparation for skin prepared by varying the concentration of the cosmetic composition for skin moisturizing according to the present invention.
FIG. 4 is a graph showing cytotoxicity according to the concentration (ppm) of the cosmetic composition for skin moisturizing according to the present invention.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Example 1: Selection of natural products having moisturizing effect

Based on the results of previous studies, 20 kinds of extracts were selected and the moisturizing effect was compared and verified.

1-1. Manufacture of natural extract

The plant parts shown in the following Table 1 were collected and cleaned thoroughly to remove impurities and impurities, shaken at 20 to 35 ° C, and pulverized to a particle size of 1 mm or less. Then, 100 g of each of the pulverized powders was immersed in 10 L of 70% ethanol and sonicated for 24 hours. Then, the obtained extract was filtered through a filter paper (Advantes, No. 2) and concentrated under reduced pressure to prepare respective extracts.

1-2. Moisturizing effect black

Moisture level of plant extracts was measured using MPA9 (corneometer, CK electronic, Germany) for the measurement of transepidermal water loss (TEWL) of each plant extract extracted in Example 1-1. The plant extracts were applied to a patch (adhesive plaster, Ripafet Pharma Co., Ltd., Japan) and applied to the abdominal upper part of the experimenter and waited for 30 minutes. Then, after the patch was removed, the degree of moisturization at each application site was measured at regular time intervals, and the amount of moisturizing change was calculated using the following equation (1). All measurements were carried out in a constant temperature and humidity chamber maintained at 22 ° C and 40% relative humidity, without air movement and no direct sunlight. The results are shown in Table 1 below.

Test substance TEWL (g / cm ³⁾ Control group 16.8 Chrysanthemum extract 15.5 Wild yam root extract 14.9 Licorice Root Extract 16.1 Aloe vera leaf extract 13.2 Ginkgo leaf extract 14.8 Chestnut pine bark extract 14.2 Mulberry bark extract 15.3 Magnolia bark extract 14.8 Magnolia flower extract 15.3 Chrysanthemum flower extract 15.1 Camphor tree branch / leaf extract 13.1 Sausage cactus extract 8.2 Strawberry outpost extract 13.2 Basil seed extract 12.9 Chestnut tree branch / leaf extract 14.2 Iris root extract 14.7 Fowl worm / leaf extract 14.5 Peppermint leaf extract 15.3 oatmeal 15 Eucalyptus leaf extract 15

As a result of the test, 20 kinds of plant extracts were selected as the natural extract of the present invention.

Test Example 1: Measurement of moisturizing effect of panthenol

The moisturizing effect of panthenol was measured in the same manner as in Example 1-2. Patches not treated with panthenol were used as controls. After removing the patch, the degree of moisturization at each application site was measured after a certain time. The results are shown in Table 2 below.

Test substance TEWL (g / cm ³⁾ Control group 17 One% 15.1 2% 14.3 3% 12.8

Test Example 2: Measurement of moisturizing effect of hyaluronic acid

The moisturizing effect of hyaluronic acid was measured in the same manner as in Example 1-2. As a control group, patches not treated with hyaluronic acid were used. After removing the patch, the degree of moisturization at each application site was measured after a certain time. The results are shown in Table 3 below.

Test substance TEWL (g / cm ³⁾ Control group 16.3 0.01% 14.8 0.05% 13.7 0.1% 12.1

Test Example 3: Measurement of moisturizing effect of betaine

The moisturizing effect of betaine was measured in the same manner as in Example 1-2. As a control group, patches not treated with betaine were used. After removing the patch, the degree of moisturization at each application site was measured after a certain time. The results are shown in Table 4 below.

Test substance TEWL (g / cm ³⁾ Control group 17.2 One% 15.1 2% 13.9 3% 11.5

Example 2: Preparation of composite compound according to the present invention

2-1. Composite compound manufacturing

Panthenol, hyaluronic acid and betaine, which are the moisturizing components identified in Test Examples 1 to 3, were mixed at various ratios to prepare a composite compound. The composition ratio of the composite compound was prepared in ten composition ratios as shown in Table 5 below.

Complex compound number Panthenol Hyaluronic acid Betaine One 0.5 0.5 0.5 2 0.5 One 0.5 3 One 0.5 One 4 One One 0.5 5 1.5 0.5 0.5 6 1.5 One 0.5 7 0.5 1.5 0.5 8 0.5 One 1.5 9 One 1.5 One 10 One 0.5 1.5

2-2. Measurement of moisturizing effect of compound

The moisturizing effect of each compound compound prepared in Example 2-1 was measured in the same manner as in Example 1-2. Each compound was prescribed 0.1%, and the control group was a patch not treated with complex compound. After removing the patch, the degree of moisturization at each application site was measured at regular time intervals. The results are shown in Fig. 1 and Table 6 below.

Test substance TEWL (g / cm ³⁾ Control group 17.3 1 (0.1%) 13.9 2 (0.1%) 14.5 3 (0.1%) 12.2 4 (0.1%) 13.5 5 (0.1%) 13.4 6 (0.1%) 14.3 7 (0.1%) 13.8 8 (0.1%) 10.3 9 (0.1%) 12.9 10 (0.1%) 15.8

As a result of the experiment, when the patch containing the composite compound prepared according to the composition ratio was applied, the patch containing the composite compound (8) prepared by mixing the panthenol, hyaluronic acid and betaine in a weight ratio of 0.5: 1: 1.5 When applied, TEWL value was 10.3, the lowest change. Next, when a patch containing the complex compound (3) prepared by mixing panthenol, hyaluronic acid and betaine in a weight ratio of 1: 0.5: 1 was applied, the water change amount had a TEWL value of 12.2. When the patch containing the composite compound (9) prepared by mixing panthenol, hyaluronic acid and betaine in a weight ratio of 1: 1.5: 1 was applied, the water change amount was found to be 12.9. As a result of the experiment of the present Example 2-2, it was found that the complex compound according to the present invention shows remarkable skin moisturizing effect even in a much smaller amount than when panthenol, hyaluronic acid or betaine alone was used.

Example 3: Establishment of composition ratio of cosmetic composition for skin moisturizing according to the present invention

3-1. Manufacture of cosmetic composition for skin moisturizing

A cosmetic composition for skin moisturizing was prepared by using a complex compound obtained by mixing the extract of bovine cactus fruit, panthenol, hyaluronic acid and betaine prepared in Example 1 at the optimum ratio as obtained in Example 5 as an active ingredient. The composition according to Example 3 was prepared in four composition ratios as shown in Table 7 below.

Composition number Strawberry Cactus Fruit Extract Panthenol Hyaluronic acid Betaine #One 0.5 0.5 One 1.5 #2 One 0.5 One 1.5 # 3 1.5 0.5 One 1.5 #4 2 0.5 One 1.5

3-2. Moisturizing effect measurement of cosmetic composition for skin moisturizing

The moisturizing effect of each composition prepared according to Example 3-1 was measured in the same manner as in Example 1-2. Each composition was formulated in 0.1% form and a patch not treated with the composition was used as a control. After removing the patch, the degree of moisturization at each application site was measured at regular time intervals. The results are shown in FIG. 2 and Table 8.

Test substance TEWL (g / cm ³⁾ Control group 16.2 # 1 (0.1%) 14.9 # 2 (0.1%) 10.5 # 3 (0.1%) 7.3 # 4 (0.1%) 9.2

As a result of the experiment, it was found that when the patch containing the compound (# 3) prepared by mixing the bovine cactus fruit extract, panthenol, hyaluronic acid and betaine in a weight ratio of 1.5: 0.5: 1: The lowest change. When the patch containing the complex compound (# 4), which was prepared by mixing the extracts of pectin, panthenol, hyaluronic acid and betaine in a weight ratio of 2: 0.5: 1: 1.5 was applied, the water change amount had a TEWL value of 9.2 Respectively. The amount of water change was 10.5 when a patch containing a compound (# 2), which was prepared by mixing southenberry cactus fruit extract, panthenol, hyaluronic acid and betaine in a weight ratio of 1: 0.5: 1: 1.5, was applied.

3-3. Measurement of moisturizing effect according to concentration of cosmetic composition for skin moisturizing

The moisturizing effect according to the concentration of the composite compound (# 3) having the best moisturizing effect according to Example 3-2 was measured in the same manner as in Example 1-2. The concentration of the composition containing the complex compound (# 3) was 0.01%, 0.05%, and 0.1%, and as a control, a patch not treated with the composition was used. After removing the patch, the degree of moisturization at each application site was measured after a certain time. The results are shown in Fig.

As shown in FIG. 3, it was confirmed that the moisturizing effect of the cosmetic composition for skin moisturizing according to the present invention increases as the concentration increases.

3-4. conclusion

In general, when a composition containing an active ingredient of a complex compound prepared by mixing pectinol, hyaluronic acid, and betaine with an extract of fruit of a sainfoin of the United States of America is treated, high efficacy is exhibited compared with the case where only one kind of one component of the above components is treated And that the composition exhibiting an ideal synergistic effect in the moisturizing effect was mixed at a composition ratio of 1.5: 0.5: 1: 1.5.

Test Example 4: Cytotoxicity test of compound

The most effective cosmetic composition for skin moisturizing (MultiEx SuperMoist) revealed in Example 3 was examined for stability to human body. Specifically, cytotoxicity test (MTT) was performed using HaCaT cells, which are human keratinocyte cell lines. HaCaT cells were treated with the composition of Example 3 (MultiEx SuperMoist) at concentrations of 0, 10, 25, 50, 100, 250, 500, 1,000 and 2,000 占 퐂 / ml, (cytotoxic effect) was measured by MTT (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide) analysis method. The results are shown in Table 9 and Fig.

Cosmetic composition for skin moisturizing
Concentration (/ / ml) 0 5 10 25 50 100 250 500 1000 2000 Cell survival rate 100 101.2 100.8 99.7 98.4 99.5 100.2 100.3 98.6 98.2

As a result, it was confirmed that the cosmetic composition for skin moisturizing according to the present invention (MultiEx SuperMoist) does not show cytotoxicity at a concentration of 2000 μg / ml or less.

Example 4: Preparation of external preparation for skin including MultiEx SuperMoist for skin moisturizing composition

In order to confirm that the cosmetic composition for skin moisturizing according to the present invention (MultiEx SuperMoist) is safe for human skin, a skin external preparation containing a cosmetic composition for skin moisturizing (MultiEx SuperMoist) was prepared by the ingredients and contents shown in Table 10 below, A stability test was conducted. First, purified water, glycerin, and butylene glycol were mixed and dissolved at 70 캜 (water part), and the other components except for the above three components and trimethanolamine were dissolved at 70 캜 (oil part). The oil part was added to a water part and stirred with a homomixer (Tokushu Kika, Japan) for primary emulsification, and trimethanolamine was finally added thereto. Next, the bubbles generated in the mixed solution were removed, and the mixture was cooled to room temperature to prepare an external preparation for skin.

ingredient content (%) Control group Test group 1 Test group 2 Purified water 72 72 72 glycerin 8.0 8.0 8.0 Butylene glycol 4.0 4.0 4.0 Cosmetic composition for skin moisturizing
(MultiEx SuperMoist) 0 0.1 1.0 Caprylic Capri Triglyceride 8.0 8.0 8.0 Squalane 5.0 5.0 5.0 Cetearyl glucide 1.5 1.5 1.5 Sorbitan stearate 0.4 0.4 0.4 Cetearyl alcohol 1.0 1.0 1.0 Trimethanolamine 0.1 0.1 0.1 Gross weight 100 100 100

Test Example 5: Test for confirming safety of human skin for cosmetic composition for skin moisturizing according to the present invention

In the cytotoxicity test of Test Example 4, the cosmetic composition for skin moisturizing (MultiEx SuperMoist) according to the present invention proved to be free from cytotoxicity, so that a skin safety test was conducted to confirm whether it was safe for human skin. As a test method, a normal cumulative skin irritation test was performed.

The skin external preparation prepared by the method of Example 4 was applied to 30 healthy adults for 9 times and 24 hours cumulative pelletization on the upper forearm every other day, and skin cosmetic composition (MultiEx SuperMoist) We measured whether or not to give. As a control, a skin external preparation not containing a cosmetic composition for skin moisturizing (MultiEx SuperMoist) was used as a squalane base.

A pin chamber (Finn chamber, Epitest Ltd, Finland) was used as a method of applying the coating, and 15 μl of each of the external preparations was dropped into the chamber. The degree of the skin reaction on each occasion was scored using the following Equation 1, and the results are shown in Table 11 below.

At this time, a score of ± is given as 1, a score of 2 is given as +, and a score of 4 is given as ++, and a safe composition is judged when the average degree of reaction is less than 3.

Test substance Number of subjects who responded (persons) Average
Reactivity 1 week 2 weeks 3 weeks Primary Secondary Third Primary Secondary Third Primary Secondary Third ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ Control group - / - / - - / - / - - / - / - - / - / - - / - / - - / - / - - / - / - - / - / - - / - / - 0.00 Test group 1 - / - / - - / - / - - / - / - - / - / - - / - / - - / - / - - / - / - - / - / - - / - / - 0.00 Test group 2 - / - / - - / - / - - / - / - - / - / - - / - / - - / - / - - / - / - - / - / - - / - / - 0.00 Subject 30 30 30 30 30 30 30 30 30

As a result of the experiment, there was no reaction in 0, 0, 0 persons in the control group, the test group 1 and the test group 2, respectively. As a result of calculation according to the above-mentioned formula, the average degree of reactivity of the control group and the test groups 1 and 2 was 0.00 or less, all of which was 3 or less. Thus, the skin cosmetic composition (MultiEx SuperMoist) according to the present invention was judged as a safe composition.

Formulation Example 1: Formulation of cosmetic preparation

1-1. Production of flexible lotion

As shown in Table 12 below, a cosmetic composition for skin moisturizing (Flexible lotion containing MultiEx SuperMoist as an active ingredient was prepared by a conventional method.

ingredient Content (% by weight) Cosmetic composition for skin moisturizing (MultiEx SuperMoist) 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 ethanol 10.0 Triethanolamine 0.1 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

1-2. Manufacture of nutrition lotion

As shown in the following Table 13, a nutritional lotion containing the cosmetic composition for skin moisturizing (MultiEx SuperMoist) as an active ingredient was prepared according to a conventional method.

ingredient Content (% by weight) Cosmetic composition for skin moisturizing (MultiEx SuperMoist) 0.1 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 5.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

1-3. Manufacture of nutrition cream

A nutritional cream containing the cosmetic composition for skin moisturizing (MultiEx SuperMoist) as an active ingredient was prepared according to a conventional method as shown in Table 14 below.

ingredient Content (% by weight) Cosmetic composition for skin moisturizing (MultiEx SuperMoist) 0.1 Wax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

1-4. Manufacture of massage cream

As shown in Table 15 below, a massage cream containing the cosmetic composition for skin moisturizing (MultiEx SuperMoist) as an active ingredient was prepared by a conventional method.

ingredient Content (% by weight) Cosmetic composition for skin moisturizing (MultiEx SuperMoist) 0.1 Wax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

1-5. Manufacture of packs

A pack containing the cosmetic composition for skin moisturizing (MultiEx SuperMoist) as an active ingredient was prepared according to a conventional method as shown in Table 16 below.

ingredient Content (% by weight) Cosmetic composition for skin moisturizing (MultiEx SuperMoist) 0.1 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 Allantoin 0.1 ethanol 5.0 Nonyl phenyl ether 0.3 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

Claims (5)

Opuntia Ficus-Indica fruit extract; And a complex compound of panthenol, hyaluronic acid and betaine as an active ingredient. The method according to claim 1,
Wherein the complex compound comprises panthenol, hyaluronic acid and betaine in a weight ratio of 0.4 to 0.6: 0.9 to 1.1: 1.4 to 1.6. The method according to claim 1,
Wherein the composition comprises a bacteriostatic extract and a complex compound in a weight ratio of 1.4 to 1.6: 2.7 to 3.3. The method according to claim 1,
Wherein the effective ingredient is 0.0001 to 20% by weight based on the total weight of the composition. The method according to claim 1,
The composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Wherein the composition is a formulation.

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