KR102334348B1 - Cosmetic Composition For Moisturizing Skin Comprising Mixed Extract of Petroselinum Crispum, Houttuynia Cordata, Mentha Piperita Leaf and Morinda Citrifolia as Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed extract of parsley, yam wheat, peppermint leaf and noni as an active ingredient, and more particularly, to a cosmetic composition for moisturizing the skin excellent in skin moisturizing effect and skin barrier improvement effect.

Description

Cosmetic Composition For Moisturizing Skin Comprising Mixed Extract of Petroselinum Crispum, Houttuynia Cordata, Mentha Piperita Leaf and Morinda Citrifolia as Active Ingredient }

The present invention relates to a cosmetic composition containing a mixed extract of parsley, yak buckwheat, peppermint leaf and noni as an active ingredient, and more particularly, to a cosmetic composition for moisturizing.

The goal of cosmetics is to maintain beautiful and healthy skin, and to delay the aging of the skin that occurs naturally with aging. In general, aging is caused by oxidative damage to biological materials by free radicals. That is, highly reactive free radicals such as active oxygen such as hydrogen peroxide, hydroxy radicals, and superoxide radicals peroxidize lipids constituting cell membranes to destroy cell membranes, oxidize proteins, and attack hexane to cause oxidative damage, biomaterials destroys the cells and eventually causes the cells to die. The most significant skin characteristics due to aging include wrinkles, loss of elasticity, and dullness of the skin.

In addition, in addition to wrinkles and decreased elasticity, after the age of 40, the color of the face is not clear and often dull and dark. In particular, in the case of young women, eohyeol occurs during childbirth and blood circulation disorders occur, which leads to a decrease in metabolic ability, and causes the skin to become rough and dull. When the metabolic ability decreases and the skin becomes rough, the moisture content is also easily reduced. In general, the moisture content of the skin is about 70% in the dermis layer, but decreases toward the epidermis layer and becomes about 10 to 30% in the keratinocyte layer. It is known that a substance with hydrophilic water retention ability called Natural Moisturizing Factor (NMF) exists in the keratinocyte layer and plays an important role in moisturizing the skin. When the normal keratinocyte layer has about 10 to 30% moisture, the skin is smooth and soft, and the body's protective function is normally exhibited.

Therefore, proper maintenance of the moisture content of the keratinocyte layer is very important to prevent skin aging, but other natural moisturizing factors such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate (Sodium lactate), urea, etc. There is a problem of impairing the emulsion stability of this strong cosmetic. Although amino acids, collagen, and elastin have moisturizing properties, there is a limit to their moisturizing ability. The ability to retain moisture in the stratum corneum can be controlled by the natural moisturizing factor (NMF), which consists of sebum components, amino acids, lactic acid, urea, and inorganic salts. The development of substances with high moisturizing effect is one of the important research tasks in cosmetics. In addition, substances known to have an antioxidant action include ascorbic acid, dl-alpha-tocopherol, beta-carotene (β-carotene), dibutylhydroxyltoluene (BHT), and the like. , Since these substances are used as synthetic products, they may cause skin safety problems when used for a long period of time or in large amounts, so the development of new antioxidant cosmetics that can be safely and effectively applied to the skin is urgently required.

Therefore, research on skin aging and moisture improvement is expected to continue, and many cosmetics using natural products are being developed to solve these skin problems and reduce skin irritation caused by various chemicals. Natural ingredients have fewer side effects on the skin, and as consumers' response to cosmetics using natural ingredients increases, the value of development as a cosmetic raw material is increasing.

For this purpose, research is being actively conducted to search for the physiological activity of various natural plant ingredients and to develop substances with excellent safety and excellent skin-beautifying effects.

Korean Patent No. 10-1926783 discloses a cosmetic composition for moisturizing the skin containing a mixture of a konjac root extract, a golden berry fruit extract, and a walhamian flower extract.

In addition, Korea Patent No. 10-1940056 discloses a moisturizing cosmetic composition containing Myongwolcho extract and Boamera extract.

Accordingly, the present inventors studied the application possibilities for cosmetics in various natural materials that are not known so far, and as a result, parsley, yam wheat, peppermint leaf and noni were selected, and extracts and mixed extracts thereof were prepared and the moisturizing effect was measured. , found that the efficacy of the mixed extract was very good.

It is an object of the present invention to provide a cosmetic composition for moisturizing the skin that contains a mixed extract of parsley, yammilk, peppermint leaf, and noni, and exhibits a moisturizing effect.

In addition, it is an object of the present invention to provide a cosmetic composition exhibiting excellent moisturizing effect by preparing a supercritical and ultrasonic extract and refining it in order to maximize the efficacy of the parsley, yam wheat, peppermint leaf and noni mixed extract.

The present invention relates to a cosmetic composition for moisturizing the skin containing a mixed extract of parsley, yak buckwheat, peppermint leaf and noni.

Preferably, the cosmetic composition has an effect of improving the skin barrier function.

Also preferably, the mixed extract of parsley, yakmomil, peppermint leaf and noni may be contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.

In addition, preferably, the parsley, yakmomil, peppermint leaf and noni mixed extract (a) purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, It is characterized in that the extraction is performed by an extraction method selected from the group consisting of a solvent extraction method for extraction using one or more solvents selected from the group consisting of hexane and a mixture thereof, (b) supercritical extraction method, and (c) ultrasonic extraction method.

Also preferably, among the mixed extracts, parsley, millet, peppermint leaf, and noni are contained in a mixed weight ratio of 1 to 3:1 to 3:1 to 3:1 to 3, characterized in that it is a cosmetic composition for moisturizing the skin. do.

Also preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing agent, oil, powder foundation, emulsion foundation, wax foundation and spray. It may have any one formulation selected from

The mixed extract of parsley, buckwheat, peppermint leaf and noni according to the present invention is excellent in moisturizing effect and skin barrier function recovery effect.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and have the meanings as commonly understood by one of ordinary skill in the art of the present invention. In addition, although preferred methods and samples are described herein, similar or equivalent ones are also included in the scope of the present invention.

The term "about" means 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprises" and "comprising" include, but are not limited to, a given step or element, or group of steps or elements, but any other step or element, or It is to be understood that a step or group of elements is not excluded.

The present invention relates to a cosmetic composition for moisturizing the skin containing a mixed extract of parsley, yak buckwheat, peppermint leaf and noni.

Parsley ( Petroselinum crispum ) used as an active ingredient in the cosmetic composition of the present invention is a dicotyledonous biennial herb of the Umbilicalaceae family. Parsley is known for various effects such as diuretic action, liver function strengthening action, appetite enhancing effect, and blood vessel strengthening effect. The parsley of the present invention uses an outpost among various organs or parts of parsley (eg, leaves, flowers, roots, stems, etc.).

Houttuynia cordata is a perennial plant of the family Trifoliumaceae, and it is the only species constituting the monotype genus Yamba. It is also called eoseongcho because the leaves and stems smell like fishy meat. It has several names, such as Chinese herbal medicine, juice, and ten medicine. Yakmomil is known to be effective in antibacterial, skin health, skin diseases such as acne and eczema, and to help hair health. Yakwoomil of the present invention uses outposts among various organs or parts (eg, leaves, flowers, roots, stems, etc.) of Yakwoomil.

Peppermint ( Mentha) piperita ) is a hybrid of watermint and spearmint belonging to the genus Mint. It is native to Europe, but today it is cultivated all over the world. Peppermint is used today as a variety of additives such as tea, ice cream, sweets, gum, and toothpaste, and is known to have various effects on allergy relief, oral health, and gastrointestinal health. In the present invention, peppermint leaves are used.

Noni ( Morinda citrifolia ) is an evergreen shrub belonging to the genus Noniaceae. Depending on the region, it is also called Indian mulberry, bajitian, painkiller tree, cheese fruit, and pageukcheon. The fruit of noni is widely used for food and medicinal purposes. In addition, leaves, stems, flowers, and seeds are also used medicinally. . Noni's proxeronin component is good for regenerating skin damaged by UV rays. It is known to be good for gout by lowering uric acid levels. It is said to remove inflammation from the blood and is good for the joints. The noni of the present invention uses an outpost among various organs or parts of noni (eg, leaves, flowers, roots, stems, seeds, etc.).

In addition, a mixed extract of parsley, yak buckwheat, peppermint leaf and noni may be prepared according to a conventional method known in the art. For example, extraction is performed using (a) solvent extraction, (b) supercritical extraction using reduced pressure and high temperature with carbon dioxide, or (c) ultrasonic extraction.

In the present invention, the mixed extract of parsley, yam wheat, peppermint leaf and noni is prepared using various extraction solvents, for example, water, anhydrous or hydrated lower alcohols having 1-4 carbon atoms (eg, methanol, ethanol, propanol and butanol), propylene It can be obtained using one or more extraction solvents selected from the group consisting of glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, nucleic acids, and mixtures thereof. and preferably obtained using ethanol, 70% (v/v) ethanol or water, and most preferably obtained using 70% (v/v) ethanol.

On the other hand, it is apparent to those skilled in the art that, for the mixed extract of parsley, yam wheat, peppermint leaf, and noni of the present invention, an extract exhibiting substantially the same effect can be obtained using other extraction solvents as well as the above-described extraction solvent.

In addition, the mixed extract of parsley, yam wheat, peppermint leaf and noni of the present invention includes not only the extract using the above-described extraction solvent, but also an extract that has undergone a conventional purification and fermentation process. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by extraction using ultrasound, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (size, charge, hydrophobicity or affinity The active fraction obtained through various purification and extraction methods additionally performed, such as separation by a method prepared for separation according to sex) or an extract using a fermentation product using a natural state or various microorganisms, is also included in the extract of the present invention.

In addition, the present invention provides a cosmetic composition, characterized in that the extract is obtained by cooling the extract at room temperature, heating a liquid obtained by filtration, and further concentrating a solvent under reduced pressure or freeze-drying.

The extraction method by the supercritical extraction method by the reduced pressure by carbon dioxide and the high temperature refers to the supercritical fluid extraction method, and in general, the supercritical fluid is a liquid having a gas when it reaches a critical point under high temperature and high pressure conditions, and It has gaseous properties and chemically has a polarity similar to that of a non-polar solvent. Due to these properties, supercritical fluids are used for the extraction of fat-soluble substances (J. Chromatogr. A. 1998;479:200-205).

Carbon dioxide becomes a supercritical fluid with both liquid and gas properties as the pressure and temperature reach the critical point through the operation of the supercritical fluid device, and as a result, the solubility in fat-soluble solutes increases. When the supercritical carbon dioxide passes through an extraction vessel containing a certain amount of the sample, the fat-soluble substance contained in the sample is extracted into the supercritical carbon dioxide.

After extracting the fat-soluble substance, if supercritical carbon dioxide containing a small amount of co-solvent is again flowed through the sample remaining in the extraction container, components that were not extracted with pure supercritical carbon dioxide alone can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract the active ingredient by using supercritical carbon dioxide or a mixed fluid in which a cosolvent is additionally mixed with carbon dioxide.

As the co-solvent, one or a mixture of two or more selected from the group consisting of chloroform, olive oil, ethanol, methanol, water, ethyl acetate, nucleic acid and diethyl ether may be used. Preferably, one or a mixture of two or more selected from the group consisting of olive oil, ethanol, methanol, and water may be used.

Most of the extracted samples contain carbon dioxide, and since carbon dioxide is volatilized into the air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the cosolvent can be removed by a vacuum evaporator.

In addition, the ultrasonic extraction method is an extraction method using energy generated by ultrasonic vibration, and ultrasonic waves can destroy an insoluble solvent contained in a sample in an aqueous solvent. Since the kinetic energy of the reactant particles is increased, sufficient energy required for the reaction is obtained, and the high pressure is induced by the impact effect of ultrasonic energy to increase the mixing effect of the material and the solvent contained in the sample, thereby increasing the extraction efficiency.

The extraction solvent that can be used for the ultrasonic extraction method may be one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, nucleic acid, and diethyl ether. The extracted sample is vacuum filtered to recover the filtrate, then removed by a vacuum evaporator, and an extract can be obtained through a conventional extract preparation method of freeze-drying.

According to the present invention, the mixed extract of parsley, millet, peppermint leaf and noni is contained in an amount of 0.001 to 30.0% by weight, preferably 0.01 to 20% by weight, more preferably 0.1 to the total weight of the cosmetic composition. It is characterized in that it contains ~ 10% by weight.

When the content of the mixed extract is less than 0.001% by weight, the skin moisturizing effect does not appear, and when it exceeds 30.0% by weight, the degree of increase in the skin moisturizing effect for the content increase is insignificant, and there is a problem with the safety and stability of the formulation. It's not economical either.

The mixed extract of parsley, buckwheat millet, peppermint leaf and noni according to the present invention may include parsley, buckwheat millet, peppermint leaf and noni extract in a mixed weight ratio of 1 to 3:1 to 3:1 to 3:1 to 3, Preferably, it may be included in a mixing weight ratio of 1:1:1:1.

In order to use the mixed extract of parsley, yammilk, peppermint leaf, and noni prepared in a conventional manner in a cosmetic formulation, after removing unnecessary components through a purification process, it may include a cosmetic composition showing efficacy. The purification process can be any conventional purification process, and liquid-liquid extraction using a difference in solubility, precipitation and a filter, and chromatography using the size of polarity and molecular weight, etc. can be used. Also, for example, after dissolving parsley, millet, peppermint leaf, and noni mixed extract in an aqueous ethanol solution (50%, 70% solution, etc.) or other aqueous solution, use a column filled with HP-20 and sephadex columns to determine polarity and molecular weight After separating the water-soluble material and the oil-soluble material using tea, the oil-soluble material is concentrated by TLC and freeze-dried to prepare a concentrate.

The ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the active ingredient as an active ingredient, for example, conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. adjuvants, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

In the case of cleansing in which the formulation of the present invention contains a surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid as carrier components Amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters can be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , cleansing lotion, cleansing water, and cleansing gel, but not limited thereto.

Hereinafter, the present invention will be described in more detail through a preparation example. These preparation examples are only for illustrating the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these preparation examples according to the gist of the present invention. .

Preparation Example 1 : Mixed extract of parsley, buckwheat, peppermint leaf and noni

After mixing 60 g each of chopped and dried parsley (outpost), buckwheat wheat (outpost), peppermint leaf and noni (outpost), it is extracted with 70% (v/v) ethanol solution at reflux 3 times for 5 hours each, and then cooled. , filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the dried parsley, yam wheat, peppermint leaf and noni mixed extract powder was dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 2 : Supercritical fluid mixed extract of parsley, yak wheat, peppermint leaf and noni

After mixing 60 g each of chopped parsley (outpost), buckwheat wheat (outpost), peppermint leaves and noni (outpost), it is mixed with a conventional supercritical extraction method (at a temperature of about 60 ° C and under a pressure of about 300 atmospheres (atm)) Extracted using olive oil as a co-solvent in a supercritical state), and after removing salts using an ultrafiltration membrane, concentrated under reduced pressure and freeze-dried to obtain 31.3 g of dried parsley, millet, peppermint leaf and noni mixed extract powder. got it This was used by dissolving it in 50% 1,3-butylene glycol.

Preparation Example 3 : Preparation of ultrasonically mixed extracts of parsley, buckwheat wheat, peppermint leaves and noni

After mixing 60 g each of chopped and dried parsley (outpost), buckwheat wheat (outpost), peppermint leaf (outpost) and noni (outpost), it is ultrasonicated (using a supernonic ultrasonic device at 25 KHz intensity at 30°C) extraction for 2 hours), and after removing salts using an ultrafiltration membrane, 7.1 g was obtained through concentration under reduced pressure and freeze-drying. Dried parsley, yak buckwheat, peppermint leaf and noni mixed extract powder was dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 4 : Parsley, yak buckwheat, peppermint leaf and noni mixed extract

After chopping and drying parsley (outpost), buckwheat wheat (outpost), peppermint leaf and noni (outpost), 96g, 48g, 48g, and 48g, respectively, were mixed, and then mixed with 70% (v/v) aqueous ethanol solution 3 times for 5 hours each. After extraction under reflux and cooling, it was filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the dried parsley, yam wheat, peppermint leaf and noni mixed extract powder was dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 5 : Parsley, yak buckwheat, peppermint leaf and noni mixed extract

Chopped and shaded parsley (outpost), buckwheat wheat (outpost), peppermint leaf and noni (outpost) were mixed with 48g, 96g, 48g, and 48g, respectively, and then mixed with 70% (v/v) ethanol solution 3 times for 5 hours each. After extraction under reflux and cooling, it was filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, dried parsley, yam wheat, peppermint leaf and noni mixed extract powder was dissolved in 50% (v/v) 1,3-butylene glycol and used.

Preparation Example 6 : Parsley, yak buckwheat, peppermint leaf and noni mixed extract

Chopped and shaded parsley (outpost), buckwheat wheat (outpost), peppermint leaf, and noni (outpost) were mixed with 48g, 48g, 96g, and 48g, respectively, and then mixed with 70% (v/v) aqueous ethanol solution 3 times for 5 hours each. After extraction under reflux and cooling, it was filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, dried parsley, yam wheat, peppermint leaf and noni mixed extract powder was dissolved in 50% (v/v) 1,3-butylene glycol and used.

Preparation Example 7 : Parsley, yak buckwheat, peppermint leaf and noni mixed extract

Chopped and dried parsley (outpost), buckwheat wheat (outpost), peppermint leaf, and noni (outpost) were mixed with 48g, 48g, 48g, 96g, respectively, and then mixed with 70% (v/v) aqueous ethanol solution 3 times for 5 hours each. After extraction under reflux and cooling, it was filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the dried parsley, yam wheat, peppermint leaf and noni mixed extract powder was dissolved in 50% (v/v) 1,3-butylene glycol and used.

Comparative Preparation Examples 1 to 4 : Preparation of parsley extract, buckwheat extract, peppermint leaf extract and noni extract

After preparing 240 g each of parsley (outpost), minced buckwheat (outpost), peppermint leaf and noni (outpost), chopped and dried in the shade, each was extracted under reflux 3 times for 5 hours with 70% (v/v) aqueous ethanol solution and cooled. Then, it was filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain each extract. Then, the individual mixed extract powder of the dried parsley extract (Comparative Preparation Example 1), yak buckwheat extract (Comparative Preparation Example 2), peppermint leaf extract (Comparative Example 3) and noni extract (Comparative Preparation Example 4) was added to 50% (v /v) was used by dissolving in 1,3-butylene glycol.

Formulation Example 1 : Preparation of a cosmetic composition containing parsley, yak buckwheat, peppermint leaf and noni mixed extract

Cosmetics (Formulation Examples 1 to 7) each comprising a mixed extract of parsley, yak buckwheat, peppermint leaf and noni of Preparation Examples 1 to 7 were prepared as shown in the composition of Table 1 below. In addition, for comparison of efficacy, a cosmetic composition containing glycerin and 1,3-butylene glycol as a moisturizer instead of parsley, yam wheat, peppermint leaf, and noni mixed extract (Comparative Formulation Example 1), which does not contain any extract or moisturizer A cosmetic composition (Comparative Formulation Example 2), a cosmetic composition (Comparative Formulation Examples 3 to 6) comprising a single extract of parsley, yammilk, peppermint leaf, and noni was prepared as shown in Table 1 below.

ingredient
(weight%) Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 Formulation Example 7 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Comparative Formulation Example 4 Comparative Formulation Example 5 Comparative Formulation Example 6 Preparation Example 1 5.0 - - - - - - - - - - - - Preparation 2 - 5.0 - - - - - - - - - - - Preparation 3 - - 5.0 - - - - - - - - - - Preparation 4 - - - 5.0 - - - - - - - - - Production Example 5 - - - - 5.0 - - - - - - - - Preparation 6 - - - - - 5.0 - - - - - - - Preparation 7 - - - - - - 5.0 - - - - - - Comparative Preparation Example 1 - - - - - - - - - 5.0 - - - Comparative Preparation Example 2 - - - - - - - - - - 5.0 - - Comparative Preparation Example 3 - - - - - - - - - - - 5.0 - Comparative Preparation Example 4 - - - - - - - - - - - - 5.0 glycerin - - - - - - - 5.0 - - - - - 1.3 Butylene Glycol - - - - - - - 5.0 - - - - - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 microcrystallin 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 squalane 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 liquid paraffin 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 trioctanoin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 polysorbate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Sorbitan Stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl Acetate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 fragrance, preservative appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Experimental Example 1 : Skin electrical conductivity test

The clinical moisturizing effect and lasting power of the cosmetic compositions of Formulation Examples 1 to 7 were measured as follows. Through the survey, 100 healthy adult males and females who feel dry skin were randomly formed into groups of 25, and each of Formulation Examples 1 to 7 and Comparative Formulation Examples 1 to 6 suitable for each group were administered twice a day, twice a day for 4 weeks. was applied to both faces one by one. The moisturizing effect was evaluated by quantifying the increase rate (%) of the skin electrical conductivity according to the amount of epidermal moisture using Corneometer CM 820 (Corage + Khazaka, Germany) for 2 weeks from the start of the actual use test, and the improvement effect after the end. The experimental results are shown in Table 2 below.

division before use 2 weeks after use 4 weeks after use Formulation Example 1 25.55 40.62 50.12 Formulation Example 2 23.92 39.97 47.25 Formulation Example 3 24.81 41.23 49.82 Formulation Example 4 24.32 36.79 45.38 Formulation Example 5 21.81 37.52 43.25 Formulation Example 6 22.45 34.26 42.93 Formulation Example 7 23.02 25.62 39.90 Comparative Formulation Example 1 25.25 36.38 42.13 Comparative Formulation Example 2 23.13 24.80 26.98 Comparative Formulation Example 3 23.12 28.52 30.02 Comparative Formulation Example 4 23.87 26.30 28.64 Comparative Formulation Example 5 22.84 27.20 29.81 Comparative Formulation Example 6 24.02 28.96 32.43

As shown in Table 2 above, as comparative formulations, cosmetics containing a chemical moisturizer (Comparative Formulation Examples 1 and 2), parsley extract (Comparative Preparation Example 1), Yakmomil extract (Comparative Preparation Example 2), peppermint leaf extract (comparative Preparation Example 3) and a cosmetic containing a mixed extract of parsley, buckwheat, peppermint leaf, and noni of the present invention (comparative formulation examples 3 to 6), respectively, as compared to a cosmetic (comparative formulation example 3 to 6) containing a noni extract (comparative preparation example 4) It was confirmed that the moisturizing effect of Examples 1 to 7) was more excellent. In addition, when comparing Formulation Example 1 and Formulation Examples 4 to 7, it was confirmed that mixing at a mixing ratio of 1:1:1:1 was most preferable since the efficacy of Formulation Example 1 was excellent.

Experimental Example 2 : Skin barrier improvement effect

This study was conducted on 25 healthy women indoors at 25℃, 45% relative humidity, and no air flow. Skin barrier damage was induced using a 1% aqueous solution of sodium lauryl sulfate (SLS, Sodium Lauryl Sulfate), and after applying the cosmetic compositions of Formulation Examples 1, 2, and 3 to the upper arm of the arm, TEWAMETER TM210 (C + K eletronic GmhH. Germany) was used to measure the amount of transdermal water loss. That is, the skin barrier recovery rate (%) was confirmed by measuring the TEWL (Transepidermal Water Loss) value according to the change in TEWL value over time. The experimental results are shown in Table 3 below.

division 2D 4D 6D 8D Formulation Example 1 51.68 64.81 82.12 95.35 Formulation Example 2 51.06 65.89 87.34 98.05 Formulation Example 3 50.72 65.32 81.63 97.12 Formulation Example 4 44.12 53.31 67.80 77.35 Formulation Example 5 45.41 56.68 69.31 79.17 Formulation Example 6 43.32 56.15 67.32 69.80 Formulation Example 7 37.50 50.34 55.93 67.35 Comparative Formulation Example 1 38.81 49.29 54.55 63.69 Comparative Formulation Example 2 39.62 58.63 62.61 71.66 Comparative Formulation Example 3 38.24 51.68 60.57 70.34 Comparative Formulation Example 4 40.02 49.98 58.65 69.75 Comparative Formulation Example 5 39.84 50.12 61.73 71.82 Comparative Formulation Example 6 42.14 53.02 64.08 78.54

As shown in Table 3, Comparative Formulation Examples 1 and 2 or Parsley in Formulation Examples 1 to 7 containing parsley, yak wheat, peppermint leaf and noni mixed extract did not contain young parsley, yak buckwheat, peppermint leaf and noni mixed extract Cosmetics (Comparative Preparation Examples 3 to 6) each comprising an extract (Comparative Preparation Example 1), Yamba wheat extract (Comparative Preparation Example 2), peppermint leaf extract (Comparative Preparation Example 3) and a noni extract (Comparative Preparation Example 4) compared to It can be seen that the amount of transdermal water loss was improved. In addition, when comparing Formulation Example 1 and Formulation Examples 4 to 7, it was confirmed that mixing at a mixing ratio of 1:1:1:1 was most preferable since the efficacy of Formulation Example 1 was excellent.

Experimental Example 3 : Safety Test

A human skin patch test was performed to determine whether the cosmetic compositions of Formulation Examples 1 to 7 were irritating to the skin. Comparative Formulation Example 1 which did not show hypersensitivity to skin irritation in the past history, and did not contain parsley, buckwheat, peppermint leaf, and noni mixed extract and 2 were used as controls.

First, the test site was wiped with 70% ethanol and dried. The prepared test material was dropped into the Finn chamber (100X10, EPITEST) and then sealed and applied to the back of the test subject. The patch was applied for 24 hours, and 1 hour and 24 hours after the patch was removed, the presence or absence of erythema and edema at the test site was observed using a magnifying glass (8MC-150, DAZOR).

The skin reaction was determined according to the regulations (Table 4) of the International Contact Dermatitis Research Group (ICDRG), and the average skin reactivity was calculated according to Equation 1 below, and the results are shown in Table 5.

sign score Evaluation standard - 0 no response ± 0.5 faint or mild erythema + One Demarcated but mild erythema, edema and papules ++ 2 Distinct erythema, papules and vesicles +++ 3 Severe erythema and blistering, crusting

division 24 hours 48 hours Average skin reaction
(n=20) ± + ++ ± + ++ Formulation Example 1 One - - - - - 0.14 Formulation Example 2 - - - - - - 0.00 Formulation Example 3 One - - - - - 0.14 Formulation Example 4 One - - One - - 0.14 Formulation Example 5 One - - - - - 0.14 Formulation Example 6 2 - - - - - 0.28 Formulation Example 7 2 - - One - - 0.28 Comparative Formulation Example 1 One - - - - - 0.14 Comparative Formulation Example 2 2 - - - - - 0.28

As shown in Table 5, the cosmetic compositions of Formulation Examples 1 to 7 did not cause skin irritation, so it was found that there was no abnormality in the safety of the cosmetic formulations containing parsley, yammilk, peppermint leaf and noni extract.

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

A cosmetic for moisturizing the skin, containing as an active ingredient a mixed extract obtained by mixing parsley whole plant, millet, peppermint leaf and noni in a mixing weight ratio of 1:1:1:1, and containing 0.001 to 30.0% by weight based on the total weight of the cosmetic composition composition. The cosmetic composition for skin moisturizing according to claim 1, wherein the cosmetic composition has an effect of improving skin barrier function. The method according to claim 1, wherein the mixed extract of parsley whole plant, yam wheat, peppermint leaf and noni is (a) water, anhydrous or hydrated lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, One selected from the group consisting of a solvent extraction method using an extraction solvent selected from the group consisting of chloroform, butyl acetate, diethyl ether, dichloromethane, nucleic acids, and mixtures thereof, (b) supercritical extraction, and (c) ultrasonic extraction A cosmetic composition for skin moisturizing, which is extracted by the method. delete delete