KR20220057832A - Cosmetic Composition For Moisturizing Skin Comprising Mixed Extracts of Cimicifuga racemosa root, Mentha piperita leaf, Scutellaria baicalensis root, Morus alba bark, Morinda citrifolia and Petroselinum sativum as Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition containing, as an active ingredient, a mixed extract of Cimicifuga racemosa root, Mentha piperita leaf, Scutellaria baicalensis root, Morus alba bark, Morinda citrifolia, and Parsley sativum, and, more specifically provides a cosmetic composition for skin moisturization, which has excellent skin moisturization effects and skin barrier improvement effects.

Description

Cosmetic Composition For Moisturizing Skin Comprising Mixed Extracts of Cimicifuga racemosa root, Mentha piperita leaf, Scutellaria baicalensis root, Morus alba bark, Morinda citrifolia and Petroselinum sativum as Active Ingredient}

The present invention relates to a cosmetic composition containing as active ingredients a mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley, and more particularly, to a cosmetic composition for moisturizing the skin.

The goal of cosmetics is to maintain beautiful and healthy skin, and to delay the aging of the skin that occurs naturally with aging. In general, aging is caused by oxidative damage to biological materials by free radicals. That is, highly reactive free radicals such as active oxygen such as hydrogen peroxide, hydroxy radicals, and superoxide radicals peroxidize lipids constituting cell membranes to destroy cell membranes, oxidize proteins, and attack hexane to cause oxidative damage, biomaterials destroys the cells and eventually causes the cells to die. The most significant skin characteristics due to aging include wrinkles, loss of elasticity, and dullness of the skin.

In addition, in addition to wrinkles and decreased elasticity, after the age of 40, the color of the face is not clear and often dull and dark. In particular, in the case of young women, eohyeol occurs during childbirth and blood circulation disorders occur, which leads to a decrease in metabolic ability, and causes the skin to become rough and dull. When the metabolic ability decreases and the skin becomes rough, the moisture content is also easily reduced. In general, the moisture content of the skin is about 70% in the dermis layer, but decreases toward the epidermis layer and becomes about 10 to 30% in the keratinocyte layer. It is known that a substance with hydrophilic water retention ability called Natural Moisturizing Factor (NMF) exists in the keratinocyte layer and plays an important role in moisturizing the skin. When the normal keratinocyte layer has about 10 to 30% moisture, the skin is smooth and soft, and the body's protective function is normally exhibited.

Therefore, proper maintenance of the moisture content of the keratinocyte layer is very important to prevent skin aging, but other natural moisturizing factors such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate (Sodium lactate), urea, etc. There is a problem of impairing the emulsion stability of this strong cosmetic. Although amino acids, collagen, and elastin have moisturizing properties, there is a limit to their moisturizing ability. The ability to retain moisture in the stratum corneum can be controlled by the natural moisturizing factor (NMF), which consists of sebum components, amino acids, lactic acid, urea, and inorganic salts. The development of substances with high moisturizing effect is one of the important research tasks in cosmetics. In addition, substances known to have antioxidant activity include ascorbic acid, dl-alpha-tocopherol, beta-carotene (β-carotene), dibutylhydroxyltoluene (BHT), and the like. Since these substances are used as synthetic products, they can cause problems in skin safety when used for a long period of time or in large amounts, so the development of a new antioxidant cosmetic that can be safely and usefully applied to the skin is urgently required.

Therefore, research on skin aging and moisturizing improvement is expected to continue, and many cosmetics using natural products are being developed to solve these skin problems and reduce skin irritation caused by various chemicals. Natural ingredients have fewer side effects on the skin, and as consumers' response to cosmetics using natural ingredients increases, the value of development as a cosmetic raw material is increasing.

For this purpose, research to develop substances with excellent safety and excellent skin-beauty effects by searching the skin physiological activity of various natural plant components is currently being actively conducted.

Korean Patent Registration No. 10-2032873 discloses a cosmetic composition comprising a mixed extract containing chrysanthemum extract, lotus flower extract, and perilla leaf extract, and having antioxidant and moisturizing functions.

Accordingly, the present inventors studied the possibility of application to cosmetics in various natural materials that were not known so far, and as a result, Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley were selected, and extracts were prepared therefrom to moisturize As a result of measuring the effect, it was found that the efficacy as a cosmetic can be expected because the efficacy is very good.

It is an object of the present invention to provide a cosmetic composition for skin protection that contains a mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley to exhibit a moisturizing effect.

In addition, it is an object of the present invention to produce a supercritical, ultrasonic and fermented extract in order to maximize the efficacy of the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley, and to show the efficacy effect through a purification process To provide a cosmetic composition.

The above object is achieved by a cosmetic composition for moisturizing the skin containing a mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley.

Preferably, the cosmetic composition has an effect of improving the skin barrier function.

Also preferably, the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley may be contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.

In addition, preferably, the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley (a) purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, By an extraction method selected from the group consisting of chloroform, ethyl ether, butylene glycol, hexane and at least one solvent selected from the group consisting of a solvent extraction method, (b) supercritical extraction method and (c) ultrasonic extraction method It is characterized in that it is extracted.

Also preferably, the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley is a mixture of 1-3:1-3:1-3:1-3:1-3:1-3 It is characterized in that it is a cosmetic composition for moisturizing the skin, which is contained in a weight ratio.

The mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley according to the present invention has excellent skin moisturizing effect and skin barrier function recovery effect.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and have the meanings as commonly understood by one of ordinary skill in the art of the present invention. In addition, although preferred methods and samples are described herein, similar or equivalent ones are also included in the scope of the present invention.

The term "about" means 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprises" and "comprising" include, but are not limited to, a given step or element, or group of steps or elements, but any other step or element, or It is to be understood that a step or group of elements is not excluded.

The present invention relates to a cosmetic composition for moisturizing the skin containing a mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley.

Rakemosa horseradish root (Cimicifuga racemosa) used as an active ingredient in the cosmetic composition of the present invention is a perennial plant belonging to the dicotyledonous fernaceae family. It grows in deep mountain forests. Rakemosa riding the root of the present invention is used.

Peppermint (Mentha piperita) is a type of herb called Western mint. Peppermint got its name because its scent resembles the pungent properties of pepper. Peppermint is mainly drunk as a tea or extracted from oil and used for massage for muscle pain, joint pain, neuralgia, etc. Peppermint is also used in oriental medicine to relieve heat and relieve stress. Peppermint of the present invention uses the leaves of peppermint.

Gold (Scutellaria baicalensis) is a perennial plant of the Lamiaceae family that grows on grass in the mountains and is about 20-60 cm high. Several stems come out and grow by giving up and branching. The leaves are opposite and pointed. Flowers bloom in July-August, with purple flowers forming raceme at the tip of the stem and at the tip of the branch, running to one side. One flower hangs on each leaf axil, 1.5~2.5cm long, and the bottom is cylindrical. It is a medicinal herb used in oriental medicine for diuresis, antipyretic, abdominal pain, hemostasis, jaundice, and cancer. The gold of the present invention uses the root of gold.

Morus alba is a deciduous broad-leaved tree or shrub. Mulberry trees are planted in many cultivars to grow silkworms, and mulberry leaves are used as food for silkworms. It is cultivated all over the country, and it is distributed throughout Wonsan in East Asia and temperate regions of the Northern Hemisphere. The mulberry tree of the present invention uses the bark of the mulberry tree.

Noni (Morinda citrifolia) is mainly found in the South Pacific region of Guam, Hawaii, Fiji, and New Zealand. However, due to its adaptability, it is also cultivated in China, Southeast Asia, Australia, and India. Depending on the region, it is called the Indian mulberry, bajitian, painkiller tree, and cheese fruit. In Donguibogam, it is called Haepa-geuk or Pa-geuk-cheon. Noni extract contains scopoletin, which suppresses inflammation and maintains healthy skin with antibacterial and antiviral effects. For the noni of the present invention, the fruit of noni is used.

Parsley (Petroselinum sativum) is a green, biennial herb and plant. It is found in many Middle Eastern, European and American cuisines. Parsley is cooked with E?V and has many uses similar to cilantro, but has a slightly weaker flavor. Parsley is rich in vitamins A, B1, B3, and C, minerals such as iron, calcium and magnesium, and protein, and is also rich in chlorophyll, which has a bactericidal action. Parsley is high in iron, which is good for blood circulation and anemia. Vitamins A and C also increase resistance to disease and prevent skin aging. Parsley of the present invention uses the leaves of parsley.

In addition, the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley can be prepared according to a conventional method known in the art. For example, (a) a solvent extraction method, (b) a supercritical extraction method using reduced pressure and high temperature with carbon dioxide, or (c) an ultrasonic extraction method can be used for extraction.

In the present invention, the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley is prepared using various extraction solvents, for example, water, anhydrous or hydrous lower alcohols having 1-4 carbon atoms (e.g., methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, nucleic acid, and mixtures thereof. It can be obtained using the above extraction solvent, preferably obtained using ethanol, 70% (v/v) ethanol or water, and most preferably obtained using 70% (v/v) ethanol.

On the other hand, the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley of the present invention can be obtained by using not only the above-described extraction solvent but also an extract exhibiting substantially the same effect even when other extraction solvents are used. It is obvious to those skilled in the art.

In addition, the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley of the present invention includes not only the extract using the above-mentioned extraction solvent, but also an extract that has undergone a conventional purification and fermentation process. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by extraction using ultrasound, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (size, charge, hydrophobicity or affinity The active fraction obtained through various purification and extraction methods additionally performed, such as separation by a method prepared for separation according to sex) or extracts from fermentation products using natural conditions or various microorganisms, are also included in the extract of the present invention.

In addition, the present invention provides a cosmetic composition, characterized in that the extract is obtained by cooling the extract at room temperature, heating a liquid obtained by filtration, and further concentrating a solvent under reduced pressure or freeze-drying.

The extraction method by the supercritical extraction method by the reduced pressure by carbon dioxide and the high temperature refers to the supercritical fluid extraction method, and in general, the supercritical fluid is a liquid having a gas when it reaches a critical point under high temperature and high pressure conditions, and It has gaseous properties and chemically has a polarity similar to that of a non-polar solvent. Due to these properties, supercritical fluids are used for the extraction of fat-soluble substances (J. Chromatogr. A. 1998;479:200-205).

Carbon dioxide becomes a supercritical fluid with both liquid and gas properties as the pressure and temperature reach the critical point through the operation of the supercritical fluid device, and as a result, the solubility in fat-soluble solutes increases. When the supercritical carbon dioxide passes through an extraction vessel containing a certain amount of the sample, the fat-soluble substance contained in the sample is extracted into the supercritical carbon dioxide.

After extracting the fat-soluble substance, if supercritical carbon dioxide containing a small amount of co-solvent is again flowed through the sample remaining in the extraction container, components that were not extracted with pure supercritical carbon dioxide alone can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract the active ingredient by using supercritical carbon dioxide or a mixed fluid in which a cosolvent is additionally mixed with carbon dioxide.

As the co-solvent, one or a mixture of two or more selected from the group consisting of chloroform, olive oil, ethanol, methanol, water, ethyl acetate, nucleic acid and diethyl ether may be used. Preferably, one or a mixture of two or more selected from the group consisting of olive oil, ethanol, methanol, and water may be used.

Most of the extracted samples contain carbon dioxide, and since carbon dioxide is volatilized into the air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the cosolvent can be removed by a vacuum evaporator.

In addition, the ultrasonic extraction method is an extraction method using energy generated by ultrasonic vibration, and ultrasonic waves can destroy an insoluble solvent contained in a sample in an aqueous solvent. Since the kinetic energy of the reactant particles is increased, sufficient energy required for the reaction is obtained, and the high pressure is induced by the impact effect of ultrasonic energy to increase the mixing effect of the material and the solvent contained in the sample, thereby increasing the extraction efficiency.

The extraction solvent that can be used for the ultrasonic extraction method may be one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, nucleic acid, and diethyl ether. The extracted sample is vacuum filtered to recover the filtrate, then removed by a vacuum evaporator, and an extract can be obtained through a conventional extract preparation method of freeze-drying.

According to the present invention, the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley is contained in an amount of 0.001 to 30.0% by weight, preferably 0.01 to 20% by weight, based on the total weight of the cosmetic composition. It is characterized in that it contains 0.1 to 10% by weight more preferably. When the content of the extract is less than 0.001% by weight, the skin moisturizing effect does not appear, and when it exceeds 30.0% by weight, the degree of increase in the skin moisturizing effect for the content increase is insignificant, there is a problem in safety and stability of the formulation, and economical not even an enemy

The mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley according to the present invention contains 1-3:1 to 3:1 Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley. It may be included in a mixed weight ratio of ~3:1 to 3:1 to 3:1 to 3, preferably in a mixed weight ratio of 1:1:1:1:1:1.

In order to use the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley prepared in a conventional way in a cosmetic formulation, it goes through a purification process to remove unnecessary ingredients, and then contains a cosmetic composition showing efficacy can do. The purification process can be any conventional purification process, and liquid-liquid extraction using a difference in solubility, precipitation and a filter, and chromatography using the size of polarity and molecular weight, etc. can be used. Also, for example, after dissolving the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley in an aqueous ethanol solution (50%, 70% solution, etc.) or other aqueous solution, HP-20, sephadex column After separating the water-soluble material and the oil-soluble material by using the difference in polarity and molecular weight using the packed column, the oil-soluble material is concentrated by TLC and freeze-dried to prepare a concentrate.

The ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the active ingredient as an active ingredient, for example, conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. adjuvants, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

In the case of cleansing in which the formulation of the present invention contains a surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid as carrier components Amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters can be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , cleansing lotion, cleansing water, and cleansing gel, but not limited thereto.

Hereinafter, the present invention will be described in more detail through a preparation example. These preparation examples are only for illustrating the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these preparation examples according to the gist of the present invention. .

Preparation Example 1 : Mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley

After mixing 60 g each of Rakemosa (root), peppermint (leaf), gold (root), mulberry (bark), noni (fruit) and parsley (leaf), chopped and dried in the shade, 70% (v/v) After extraction under reflux three times for 5 hours with an aqueous ethanol solution, cooling, and filtering with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the dried Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley mixed extract powder was used by dissolving in 50% 1,3-butylene glycol.

Preparation Example 2 : Supercritical fluid mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley

After minced and dried in the shade, 60 g each of Rakemosa horseradish (root), peppermint (leaf), gold (root), mulberry (bark), noni (fruit) and parsley (leaf) were mixed, and then this was mixed with the usual supercritical extraction method (approx. Extracted using olive oil as a cosolvent in a supercritical state under a pressure of about 300 atmospheres (atm) at a temperature of 60 ° C.) A mixed extract powder of dried Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley was obtained. This was used by dissolving it in 50% 1,3-butylene glycol.

Preparation Example 3 : Preparation of ultrasonic mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley

After mixing 60 g each of Rakemosa horseradish (root), peppermint (leaf), gold (root), mulberry (bark), noni (fruit) and parsley (leaf), chopped and dried in the shade, it is ultrasonically (supernonic ultrasonic device) was extracted using a 25 KHz intensity at 30° C. for about 2 hours), and after removing the salt using an ultrafiltration membrane, 7.1 g was obtained through concentration under reduced pressure and freeze-drying. A mixed extract powder of dried Rakemosa root, peppermint leaf, gold, mulberry bark, noni and parsley was dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 4: Mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley

Mix 96g, 48g, 48g, 48g, 48g, 48g each of Rakemosasamsa (root), peppermint (leaf), gold (root), mulberry (bark), noni (fruit) and parsley (leaves) that have been chopped and dried in the shade After reflux extraction with 70% (v/v) aqueous ethanol solution 3 times for 5 hours, cooled, and filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the dried Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley mixed extract powder was used by dissolving in 50% 1,3-butylene glycol.

Preparation Example 5 : Mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley

Minced and dried rakemosa (root), peppermint (leaf), gold (root), mulberry (bark), noni (fruit) and parsley (leaves) are mixed with 48g, 96g, 48g, 48g, 48g, 48g each. After reflux extraction with 70% (v/v) aqueous ethanol solution 3 times for 5 hours, cooled, and filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.9 g. Then, the mixed extract powder of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley was used by dissolving it in 50% 1,3-butylene glycol.

Preparation Example 6 : Mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley

Chopped and dried in the shade, mixed with 48g, 48g, 96g, 48g, 48g, 48g each of Rakemosasae (root), peppermint (leaf), gold (root), mulberry (bark), noni (fruit) and parsley (leaves) After reflux extraction with 70% (v/v) aqueous ethanol solution 3 times for 5 hours, cooled, and filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.6 g. Then, the dried Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley mixed extract powder was used by dissolving in 50% 1,3-butylene glycol.

Preparation Example 7 : Mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley

Chopped and dried in the shade, mixed with 48g, 48g, 48g, 96g, 48g, 48g each of Rakemosa Seungma (root), peppermint (leaf), gold (root), mulberry (bark), noni (fruit) and parsley (leaves) After reflux extraction with 70% (v/v) aqueous ethanol solution 3 times for 5 hours, cooled, and filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the dried Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley mixed extract powder was used by dissolving in 50% 1,3-butylene glycol.

Preparation 8 : Mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley

Chopped and dried in the shade, mixed with 48g, 48g, 48g, 48g, 96g, 48g each of Rakemosasamosa (root), peppermint (leaf), gold (root), mulberry (bark), noni (fruit) and parsley (leaf) After reflux extraction with 70% (v/v) aqueous ethanol solution 3 times for 5 hours, cooled, and filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.9 g. Then, the mixed extract powder of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley was used by dissolving it in 50% 1,3-butylene glycol.

Preparation Example 9 : Mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley

Chopped and dried in the shade, mixed with 48g, 48g, 48g, 48g, 48g, 96g each of Rakemosa Seungma (root), peppermint (leaf), gold (root), mulberry (bark), noni (fruit) and parsley (leaves) After reflux extraction with 70% (v/v) aqueous ethanol solution 3 times for 5 hours, cooled, and filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.6 g. Then, the dried Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley mixed extract powder was used by dissolving in 50% 1,3-butylene glycol.

Formulation Example : Preparation of a cosmetic composition containing a mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley

Cosmetics (Formulation Examples 1 to 9) each containing the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley of Preparation Examples 1 to 9 were prepared as shown in the composition of Table 1 below. In addition, for comparison of efficacy, a cosmetic composition containing glycerin and 1,3-butylene glycol as a moisturizer instead of a mixed extract of Rakemosa root, peppermint leaf, gold, mulberry bark, noni and parsley (Comparative Formulation Example 1) , a cosmetic composition (Comparative Formulation Example 2) that does not contain any extract or moisturizer was prepared as shown in Table 1 below.

ingredient
(Unit: wt%) Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 Formulation Example 7 Formulation Example 8 Formulation Example 9 Comparative Formulation Example 1 Comparative Formulation Example 2 Preparation Example 1 5.0 - - - - - - - - - - Preparation 2 - 5.0 - - - - - - - - - Preparation 3 - - 5.0 - - - - - - - - Preparation 4 - - - 5.0 - - - - - - - Preparation 5 - - - - 5.0 - - - - - - Preparation 6 - - - - - 5.0 - - - - - Preparation 7 - - - - - - 5.0 - - - - Preparation 8 - - - - - - - 5.0 - - - Preparation 9 - - - - - - - - 5.0 - - glycerin - - - - - - - - - 5.0 - 1.3 Butylene Glycol - - - - - - - - - 5.0 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 microcrystallin 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 squalane 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 liquid paraffin 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 trioctanoin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 polysorbate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Sorbitan Stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 tocopheryl acetate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 fragrance, preservative appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Experimental Example 1 : Measurement of skin electrical conductivity

The clinical moisturizing effect and lasting power of the cosmetic compositions of Formulation Examples 1 to 9 were measured as follows. Through the questionnaire, 220 healthy adult males and females who felt that their skin was dry were randomly formed into groups of 20, and Formulation Examples 1 to 9 and Comparative Formulation Examples 1 and 2 suitable for each group were administered twice a day for 4 weeks, respectively. was applied to both faces one by one. The moisturizing effect was evaluated by quantifying the increase rate (%) of the skin electrical conductivity according to the amount of epidermal moisture using Corneometer CM 820 (Corage + Khazaka, Germany) for 2 weeks from the start of the actual use test, and the improvement effect after the end. The experimental results are shown in Table 2 below.

division before use 2 weeks after use 4 weeks after use Formulation Example 1 24.76 45.11 61.36 Formulation Example 2 24.65 43.96 47.73 Formulation Example 3 23.22 22.25 23.60 Formulation Example 4 25.28 36.89 54.62 Formulation Example 5 24.90 33.54 45.75 Formulation Example 6 23.55 27.28 30.46 Formulation Example 7 24.22 32.97 35.52 Formulation Example 8 25.68 37.76 56.39 Formulation Example 9 24.22 31.27 38.71 Comparative Formulation Example 1 23.26 24.07 26.02 Comparative Formulation Example 2 25.12 25.95 26.36

As shown in Table 2 above, as a comparative formulation, as compared to a cosmetic containing a chemical moisturizer (Comparative Formulation Examples 1 and 2), Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley mixture of the present invention It was confirmed that the moisturizing effect of the cosmetics containing the extract (Formulation Examples 1 to 9) was more excellent. In addition, when comparing Formulation Example 1 and Formulation Examples 4 to 9, since the efficacy of Formulation Example 1 is excellent, it was confirmed that it is most preferable to mix each component at a mixing ratio of 1:1:1:1:1:1. .

Experimental Example 2 : Skin barrier improvement effect

This study was conducted on 25 healthy women indoors at 25℃, 45% relative humidity, and no air flow. Skin barrier damage was induced by using a 1% aqueous solution of sodium lauryl sulfate (SLS, Sodium Lauryl Sulfate), and after applying the cosmetic compositions of Formulation Examples 1 to 9 and Comparative Formulation Examples 1 and 2 to the upper arm of the arm, TEWAMETER TM210 (C+K eletronic GmhH. Germany) was used to measure the amount of transdermal water loss. That is, the skin barrier recovery rate (%) was confirmed by measuring the TEWL (Transepidermal Water Loss) value according to the change in TEWL value over time. The experimental results are shown in Table 3 below.

division Skin barrier recovery rate (%) 2D 4D 6D 8D Formulation Example 1 62.78 81.98 92.22 98.45 Formulation Example 2 61.16 67.96 89.38 92.55 Formulation Example 3 58.82 67.45 83.73 85.06 Formulation Example 4 60.23 65.22 69.88 77.56 Formulation Example 5 57.52 58.88 71.23 76.14 Formulation Example 6 69.43 69.10 69.22 72.98 Formulation Example 7 58.49 62.44 71.21 81.32 Formulation Example 8 49.68 54.14 67.13 79.97 Formulation Example 9 51.55 53.59 61.87 67.62 Comparative Formulation Example 1 30.97 41.23 45.64 40.78 Comparative Formulation Example 2 41.55 50.62 62.74 61.74

As shown in Table 3, Formulation Examples 1 to 9 containing Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and a mixed extract of parsley, Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni And it can be seen that the skin barrier recovery rate is improved compared to Comparative Formulation Examples 1 and 2 that do not contain the mixed extract of parsley. In addition, when comparing Formulation Example 1 and Formulation Examples 4 to 9, it was confirmed that it is most preferable to mix at a mixing ratio of 1:1:1:1:1:1, since the efficacy of Formulation Example 1 is excellent.

Experimental Example 3 : Safety Test

A human skin patch test was performed to check whether the cosmetic compositions of Formulation Examples 1 to 9 were irritating to the skin. The test was conducted on 20 women in their 20s and 30s who have never had a hypersensitivity reaction to skin irritation in the past and who do not currently have skin diseases or skin allergies. Comparative Formulation Examples 1 and 2 not containing the mixed extract were used as control examples.

First, the test site was wiped with 70% ethanol and dried. The prepared test material was dropped into the Finn chamber (100X10, EPITEST) and then sealed and applied to the back of the test subject. The patch was applied for 24 hours, and 1 hour and 24 hours after the patch was removed, the presence or absence of erythema and edema at the test site was observed using a magnifying glass (8MC-150, DAZOR).

The skin reaction was determined according to the regulations (Table 4) of the International Contact Dermatitis Research Group (ICDRG), and the average skin reactivity was calculated according to Equation 1 below, and the results are shown in Table 5.

sign score Evaluation standard - 0 no response ± 0.5 faint or mild erythema + One Demarcated but mild erythema, edema and papules ++ 2 Distinct erythema, papules and vesicles +++ 3 Severe erythema and blistering, crusting

division 24 hours 48 hours Average skin reaction
(n=20) ± + ++ ± + ++ Formulation Example 1 One - - - - - 0.00 Formulation Example 2 - - - - - - 0.00 Formulation Example 3 One - - - - - 0.12 Formulation Example 4 One - - One - - 0.00 Formulation Example 5 2 - - - - - 0.12 Formulation Example 6 2 - - - - - 0.12 Formulation Example 7 One - - One - - 0.00 Formulation Example 8 2 - - One - - 0.00 Formulation Example 9 One - - One - - 0.14 Comparative Formulation Example 1 2 - - - - - 0.28 Comparative Formulation Example 2 One - - One - - 0.29

As shown in Table 5, the cosmetic compositions of Formulation Examples 1 to 9 did not cause skin irritation, so the safety of the cosmetic formulation containing the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley was added. It was found that there was no abnormality.

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

Contains extracts of Cimicifuga racemosa root, Mentha piperita leaf, Scutellaria baicalensis root, Morus alba bark, Morinda citrifolia and Parsley (Petroselinum sativum). A cosmetic composition for moisturizing the skin. The cosmetic composition for skin moisturizing according to claim 1, wherein the cosmetic composition has an effect of improving skin barrier function. The cosmetic composition for skin moisturizing according to claim 1, wherein the mixed extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. The method of claim 1, wherein the mixed extract of Rakemosa root, peppermint leaf, gold, mulberry bark, noni and parsley is (a) water, anhydrous or hydrous lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, A solvent extraction method using an extraction solvent selected from the group consisting of glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, nucleic acids, and mixtures thereof, (b) supercritical extraction and (c) ultrasonic extraction A cosmetic composition for skin moisturizing, which is extracted by a method selected from the group consisting of. The method according to claim 1, wherein the extract of Rakemosa horseradish root, peppermint leaf, gold, mulberry bark, noni and parsley is 1-3:1-3:1-3:1-3:1-3:1-3 A cosmetic composition for moisturizing the skin, which is contained in a mixed weight ratio of