KR102656826B1 - Cosmetic Composition For Moisturizing Skin Comprising Mixed Extracts of Opuntia Tuna Fruit, Thuja Occidentalis Leaf and Lilium Candidum Bulb - Google Patents

Abstract

The present invention relates to a cosmetic composition for moisturizing skin containing a mixed extract of Opuntia Tuna Fruit, Thuja Occidentalis Leaf, and Lilium Candidum Bulb as active ingredients. In detail, a cosmetic composition for skin moisturizing with excellent skin moisturizing effect and skin barrier improvement effect is provided.

Description

Cosmetic composition for moisturizing skin containing mixed extracts of prickly pear fruit, arborvitae leaves, and Lilium candidum stems {Cosmetic Composition For Moisturizing Skin Comprising Mixed Extracts of Opuntia Tuna Fruit, Thuja Occidentalis Leaf and Lilium Candidum Bulb}

The present invention relates to a cosmetic composition containing prickly pear fruit, Western arborvitae leaf, and Lilium candidum scale stem extract as active ingredients, and more specifically, to a cosmetic composition for skin moisturizing.

The goal of cosmetics is to maintain beautiful, healthy skin and delay skin aging that occurs naturally as we age. In general, aging is caused by oxidative damage to biological materials caused by free radicals. In other words, highly reactive free radicals such as active oxygen such as hydrogen peroxide, hydroxy radical, and superoxide radical destroy the cell membrane by peroxidizing the lipids that make up the cell membrane, oxidize proteins, and attack hexane, causing oxidative damage and damaging biological substances. destroys and eventually causes the cell to die. The most noticeable skin characteristics due to aging are wrinkles, loss of elasticity, and dullness of the skin.

In addition, in addition to wrinkles and loss of elasticity, after the age of 40, the complexion of the face is often dull and dark. Especially in the case of young women, experiencing childbirth causes blood clots and blood circulation problems, which reduces metabolic ability and causes the skin to easily become rough and dull. When metabolic ability decreases and the skin becomes rough, the moisture content also easily decreases. Generally, the moisture content of the skin is about 70% in the dermis layer, but decreases toward the epidermis layer to about 10 to 30% in the keratinocyte layer. It is known that in the keratinocyte layer, a substance with a hydrophilic water retention ability called Natural Moisturizing Factor (NMF) exists and plays an important role in moisturizing the skin. When the moisture content of the normal keratinocyte layer is maintained at about 10 to 30%, the skin is smooth and soft and functions to protect the body normally.

Therefore, maintaining the moisture content of the skin's keratinocyte layer appropriately is very important in preventing skin aging, but other natural moisturizing factors such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate, and urea are electrolytic. There is a problem in that this strength impairs the emulsion stability of cosmetics. Amino acids, collagen, and elastin are also said to have moisturizing abilities, but their moisturizing abilities are limited. The ability to retain moisture in the stratum corneum can be controlled by natural moisturizing factors (NMF), which are composed of sebum components, amino acids, lactic acid, urea, and inorganic salts, and are safe and usable as natural moisturizing factors in external skin products. And the development of substances with high moisturizing effect is one of the important research tasks in cosmetics.

In addition, substances known to have antioxidant activity include ascorbic acid, dl-α-tocopherol, beta-carotene, and dibutylhydroxytoluene (BHT). Since these substances are used as synthetic products, they can cause skin safety problems when used for a long time or in large quantities, so there is an urgent need for the development of new antioxidant cosmetics that can be applied to the skin more safely and usefully.

Therefore, research on skin aging and moisturizing improvement is expected to continue, and many cosmetics using natural products are being developed to solve these skin problems and reduce skin irritation caused by various chemicals. Not only do natural ingredients have fewer side effects on the skin, but their development value as cosmetic raw materials is increasing as consumers' response to cosmetics using natural ingredients has recently increased.

To this end, research is currently being actively conducted to search the skin physiological activity of various natural plant ingredients to develop substances with excellent safety and skin beauty effects.

Korean Patent No. 10-1814498 discloses a cosmetic composition containing a mixed extract of eucalyptus leaves and red clover leaves, which has skin protection effects such as improving skin moisturization and relieving skin irritation.

Accordingly, the present inventors studied the applicability of various hitherto unknown natural ingredients to cosmetics, selected prickly pear fruit, Western arborvitae leaves, and Lilium Madonna scale stem, prepared extracts from them, and measured the moisturizing effect. As a result, it was discovered that the efficacy was so excellent that it could be expected to be effective as a cosmetic.

The purpose of the present invention is to provide a skin-protecting cosmetic composition that exhibits a moisturizing effect by containing the prickly pear fruit, Western arborvitae leaf, and Lilium candidum scale stem extract as a mixed extract.

The above-described problem is achieved by a cosmetic composition for moisturizing skin containing mixed extracts of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem as active ingredients.

Preferably, the cosmetic composition has an effect of improving skin barrier function.

Also preferably, the mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem may be contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.

Also preferably, the mixed extract of the prickly pear fruit, western arborvitae leaves, and Lilium candidum scale stem is mixed with (a) purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, Solvent extraction using one or more solvents selected from the group consisting of ethyl ether, butylene glycol, hexane, and mixtures thereof, (b) supercritical extraction, and (c) ultrasonic extraction. It is characterized by

Also preferably, the mixed extract of prickly pear fruit, western arborvitae leaves and Lilium candidum scale stem is contained in a mixed weight ratio of 1 to 3:1 to 3:1 to 3, and is a cosmetic composition for moisturizing the skin. It is characterized by

The cosmetic composition containing the mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem according to the present invention has excellent skin moisturizing effect and skin barrier function recovery effect.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and correspond to the meaning as generally understood by a person skilled in the art in the relevant field of the present invention. In addition, preferred methods and samples are described in this specification, but similar or equivalent methods are also included in the scope of the present invention.

The term "about" refers to a quantity, level, value, number, frequency, percent, dimension, size, amount, weight or length of 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, means a quantity, level, value, number, frequency, percentage, dimension, size, volume, weight or length that varies by 4, 3, 2 or 1%.

Throughout this specification, unless the context otherwise requires, the words "comprise" and "comprising" include a given step or component, or group of steps or components, but any other step or component, or It should be understood that this implies that no step or group of components is excluded.

The present invention relates to a cosmetic composition for moisturizing skin containing a mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem.

Prickly Pear Fruit (Opuntia Tuna Fruit), which is used as an active ingredient in the cosmetic composition of the present invention, refers to an extract from the fruit of Prickly Pear (Optunia tuna). Prickly pear is a cactus with a flat, thorny stem that belongs to the cactus family and genus Cactus. This English name specifically refers to the edible fruit of Opuntia ficus-india, also called Indian fig, which is an important food source for people living in tropical and subtropical regions. Large yellow flowers with a diameter of 7.5 to 10 cm bloom, followed by white, yellow, or reddish-purple fruits. In temperate regions, it is widely planted to obtain fruit or to be used as grain for feed, and oil can be obtained from the hard seeds.

Thuja Occidentalis Leaf is an evergreen coniferous tree of the gymnosperm coniferous family Cupressaceae and is native to North America. It reaches a height of 20 m and a diameter of 60 cm and is fragrant. The wood is of excellent quality and is used as building material, furniture material, and civil engineering material, and the leaves are used to collect spices or for medicinal purposes. It is propagated by seeds and cuttings, and many varieties have been developed in its country of origin. It is mainly planted for ornamental purposes, but when young, it is more resistant to cold than arborvitae. The bark is light yellowish brown, peels off thinly here and there, and the branches spread horizontally. The leaves are egg-shaped and suddenly pointed. The leaves on the stems are 3 mm long and have pre-spotted leaves, while the leaves on the branches do not have pre-spotted leaves. The surface is light green and the back is yellowish green. In the present invention, leaf extract of Western arborvitae can be used.

Lilium Candidum Bulb refers to the scaly stem of the Madonna lily. The genus name Lilium is a combination of the Latin words 'li' (white) and 'lium' (flower), and was named after the pure white Madona Lily known in Europe. It is a bulbous plant that grows up to 2m in height. The bulb is round with a peduncle. The peduncle is white or light yellow and has a diameter of about 10 cm. Ancient people cooked and ate bulbs, and bulb dishes are still eaten in many countries. Mucus components are used in cosmetics. Used as an ointment for burns, rashes, and acne. The oil-cured petals treat eczema, and the flowers are used in commercial perfumery.

Additionally, a mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stems can be prepared according to conventional methods known in the art. For example, extraction is performed using (a) solvent extraction, (b) supercritical extraction using reduced pressure and high temperature using carbon dioxide, or (c) ultrasonic extraction.

In the present invention, the mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem is extracted with various extraction solvents, such as water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (e.g., methanol, ethanol, Propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, nucleic acids, and mixtures thereof. It can be obtained using a solvent, preferably using ethanol, 70% (v/v) ethanol or water, and most preferably using 70% (v/v) ethanol.

Meanwhile, the mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem of the present invention shows that an extract showing substantially the same effect can be obtained using not only the above-described extraction solvent but also other extraction solvents. This is self-evident to those skilled in the art.

In addition, the mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem of the present invention includes not only extracts using the above-described extraction solvent, but also extracts that have undergone conventional purification and fermentation processes. For example, decompression with carbon dioxide, extraction by supercritical extraction at high temperature, extraction by ultrasonic extraction, separation using an ultrafiltration membrane with a certain molecular weight cut-off value, and various chromatography methods (size, charge, hydrophobicity or affinity). The extract of the present invention also includes active fractions obtained through various additional purification and extraction methods, such as separation by (manufactured for separation according to gender) or extracts from fermentation products in natural state or using various microorganisms.

In addition, the present invention provides a cosmetic composition, wherein the extract is a liquid obtained by cold immersion, heat filtration at room temperature, and further obtained by concentrating the solvent under reduced pressure or freeze-drying.

The extraction method using the supercritical extraction method using reduced pressure and high temperature using carbon dioxide refers to supercritical fluid extraction. In general, supercritical fluid is a liquid and It has the properties of a gas and has a polarity similar to that of a chemically non-polar solvent. Due to these characteristics, supercritical fluid is used for the extraction of fat-soluble substances (J. Chromatogr. A. 1998;479:200-205).

Carbon dioxide becomes a supercritical fluid with both liquid and gas properties as the pressure and temperature reach a critical point through the operation of a supercritical fluid device, and as a result, the solubility of fat-soluble solutes increases. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the fat-soluble substances contained in the sample are extracted by the supercritical carbon dioxide.

After extracting fat-soluble substances, if supercritical carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction container, components that could not be extracted with pure supercritical carbon dioxide alone can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract active ingredients by using supercritical carbon dioxide or a mixed fluid in which carbon dioxide is additionally mixed with a cosolvent.

This co-solvent may be one or a mixture of two or more selected from the group consisting of chloroform, olive oil, ethanol, methanol, water, ethyl acetate, nucleic acid, and diethyl ether. Preferably, one type or a mixture of two or more types selected from the group consisting of olive oil, ethanol, methanol, and water can be used.

Most of the extracted samples contain carbon dioxide, and since carbon dioxide volatilizes into the air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the co-solvent can be removed with a reduced pressure evaporator.

In addition, the ultrasonic extraction method is an extraction method that uses energy generated by ultrasonic vibration. Ultrasonic waves can destroy the insoluble solvent contained in the sample in the aqueous solvent, and the reactants located around due to the high local temperature generated at this time. By increasing the kinetic energy of the particles, sufficient energy required for the reaction is obtained, and the impact effect of ultrasonic energy induces high pressure, increasing the mixing effect of the substances contained in the sample and the solvent, thereby increasing extraction efficiency.

The extraction solvent that can be used in the ultrasonic extraction method can be one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, nucleic acid, and diethyl ether. The extracted sample can be obtained by vacuum filtration, recovering the filtrate, removing it with a vacuum evaporator, and freeze-drying it through a conventional extract preparation method.

According to the present invention, the mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem is contained in an amount of 0.001 to 30.0% by weight, preferably, 0.01 to 20% by weight, based on the total weight of the cosmetic composition. , more preferably 0.1 to 10% by weight. If the content of the extract is less than 0.001% by weight, no skin moisturizing effect is observed, and if it exceeds 30.0% by weight, the increase in skin moisturizing effect is minimal due to an increase in content, and there are problems with the safety and stability of the formulation and economical issues. It's not even an enemy.

The mixed extract of prickly pear fruit, western arborvitae leaves, and lily madonna scale stems according to the present invention is a mixture of prickly pear fruit, western arborvitae leaves, and lily madonna scale stems in a ratio of 1 to 3:1 to 3:1 to 3. It can be included in a weight ratio, preferably in a mixed weight ratio of 1:1:1.

In order to use the mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium madonna scale stem prepared by a conventional method in cosmetic formulations, it can be purified to remove unnecessary ingredients and then included in a cosmetic composition showing efficacy. there is. The purification process can be any conventional purification process, and liquid-liquid extraction using solubility differences, precipitation and filtering, and chromatography using polarity and molecular weight, etc. can be used. Also, for example, mixed extracts of prickly pear fruit, western arborvitae leaves, and Lilium candidum scale stems are dissolved in ethanol aqueous solution (50% (v/v), 70% (v/v) solution, etc.) or other aqueous solutions. , HP-20, sephadex columns can be used to separate water-soluble substances and oil-soluble substances using differences in polarity and molecular weight, and then concentrate the oil-soluble substances by TLC and freeze-dry them to prepare a concentrate.

The ingredients included in the cosmetic composition of the present invention are active ingredients and may include ingredients commonly used in cosmetic compositions in addition to the above-mentioned active ingredients, such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. Includes adjuvants and carriers.

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing products. , oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray, etc., but is not limited thereto. More specifically, it can be manufactured in the form of softening lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystals. Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.

In the case of a cleansing formulation containing a surfactant, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid. Amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester can be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it can be applied to the skin and then wiped off, removed, or washed with water. As a specific example, the soap is liquid soap, powdered soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is cleansing cream. , cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

Hereinafter, the present invention will be described in more detail through manufacturing examples. These manufacturing examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these manufacturing examples according to the gist of the present invention. .

Preparation Example 1 : Mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium Madonna scale stem

After mixing 60 g each of finely chopped and shade-dried prickly pear (fruit), western arborvitae (leaf), and lily madonna (scale stem), the mixture was refluxed and extracted three times for 5 hours each with a 70% (v/v) ethanol aqueous solution. After cold maceration, it was filtered through Whatman #4 filter paper. Salts were removed from the filtered extract using an ultrafiltration membrane, and then concentrated under reduced pressure and freeze-dried at 50°C or lower to obtain 6.7g. Next, the combined extract powder of dried prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem was dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 2 : Critical fluid mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium Madonna scale stem

After mixing 60g each of finely chopped and shade-dried prickly pear (fruit), western arborvitae (leaf), and lily madonna (scale stem), the mixture was subjected to conventional supercritical extraction (approximately 300 atmospheres (atm) at a temperature of approximately 60°C). Extracted using olive oil as a co-solvent under supercritical pressure), salts were removed using an ultrafiltration membrane, concentrated under reduced pressure, and freeze-dried to obtain 31.3 g of dried prickly pear fruit and western arborvitae leaves. and the sum extract powder of Lilium Madonna scale stem was obtained. This was dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 3 : Preparation of ultrasonic mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium Madonna scale stem

After mixing 60g each of finely chopped and shade-dried prickly pear (fruit), western arborvitae (leaf), and lily madonna (scale stem), the mixture was subjected to ultrasonic waves (using a Supernonic ultrasonic device) at approximately 30°C at an intensity of 25 KHz. It was extracted using (extraction for 2 hours), salts were removed using an ultrafiltration membrane, and then 7.1 g was obtained through reduced pressure concentration and freeze-drying. A mixed extract powder of dried prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem was used by dissolving it in 50% 1,3-butylene glycol.

Preparation Example 4: Mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium Madonna scale stem

Mix 90g, 45g, and 45g of finely chopped and shade-dried prickly pear (fruit), western arborvitae (leaf), and lily madonna (scale stem), respectively, and then mix them with 70% (v/v) ethanol aqueous solution for 5 hours each. After reflux extraction and cold maceration, it was filtered through Whatman #4 filter paper. Salts were removed from the filtered extract using an ultrafiltration membrane, and then concentrated under reduced pressure and freeze-dried below 50°C to obtain 6.7g. Next, the mixed extract powder of dried prickly pear (fruit), Western arborvitae (leaf), and Lilium candidum (scale stem) was used by dissolving it in 50% 1,3-butylene glycol.

Preparation Example 5 : Mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium Madonna scale stem

Mix 45g, 90g, and 45g of finely chopped and shade-dried prickly pear (fruit), western arborvitae (leaf), and lily madonna (scale stem), respectively, and then mix them with 70% (v/v) ethanol aqueous solution for 5 hours each. After reflux extraction and cold maceration, it was filtered through Whatman #4 filter paper. Salts were removed from the filtered extract using an ultrafiltration membrane, and then concentrated under reduced pressure and freeze-dried below 50°C to obtain 6.9g. Next, the mixed extract powder of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem was dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 6 : Mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium Madonna scale stem

Mix 45g, 45g, and 90g of finely chopped and shade-dried prickly pear (fruit), western arborvitae (leaf), and lily madonna (scale stem), respectively, and then mix them with 70% (v/v) ethanol aqueous solution for 5 hours each. After reflux extraction and cold maceration, it was filtered through Whatman #4 filter paper. Salts were removed from the filtered extract using an ultrafiltration membrane, and then concentrated under reduced pressure and freeze-dried below 50°C to obtain 6.6g. Next, the mixed extract powder of dried prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem was dissolved in 50% 1,3-butylene glycol and used.

Formulation example : Preparation of a cosmetic composition containing mixed extracts of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem.

Cosmetics (Formulation Examples 1 to 6) containing mixed extracts of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem of Preparation Examples 1 to 6, respectively, were prepared according to the composition in Table 1 below. In addition, for comparison of efficacy, a cosmetic composition (Comparative Formulation Example 1) containing glycerin and 1,3-butylene glycol as a moisturizing agent instead of a mixed extract of prickly pear fruit, western arborvitae leaves, and Lilium candidum scale stem, extract A cosmetic composition (Comparative Formulation Example 2) containing no moisturizer was prepared as shown in Table 1 below.

ingredient
(Unit: weight%) Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation example 4 Formulation example 5 Formulation example 6 Comparative formulation example 1 Comparative formulation example 2 Manufacturing Example 1 5.0 - - - - - - - Production example 2 - 5.0 - - - - - - Production example 3 - - 5.0 - - - - - Production example 4 - - - 5.0 - - - - Production example 5 - - - - 5.0 - - - Production example 6 - - - - - 5.0 - - glycerin - - - - - - 5.0 - 1.3Butylene glycol - - - - - - 5.0 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 microcrystalline 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 squalane 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 liquid paraffin 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Trioctanoin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 polysorbate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Sorbitan Stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl Acetate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Fragrance, preservative Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Experimental Example 1 : Measurement of skin electrical conductivity

The clinical moisturizing effect and durability of the cosmetic compositions of Formulation Examples 1 to 6 were measured as follows. Through a survey, 220 healthy adult men and women who felt that their skin was dry were randomly formed into groups of 20 people, and each group received Formulation Examples 1 to 6 and Comparative Formulation Examples 1 and 2 twice a day for 4 weeks. One side was applied to both sides of the face. The moisturizing effect was evaluated for two weeks from the start of the actual use test, and the improvement effect after the end was evaluated by quantifying the increase rate (%) of skin electrical conductivity according to the amount of epidermal moisture using Corneometer CM 820 (Corage + Khazaka, Germany). The experimental results are shown in Table 2 below.

division Before use After 2 weeks of use After 4 weeks of use Formulation Example 1 24.76 46.61 60.56 Formulation Example 2 24.65 42.98 45.75 Formulation Example 3 23.22 23.26 21.30 Formulation Example 4 25.28 38.79 52.22 Formulation Example 5 24.90 32.54 43.45 Formulation Example 6 23.55 26.26 25.16 Comparative formulation example 1 23.26 23.17 24.16 Comparative formulation example 2 25.12 26.85 27.88

As shown in Table 2, compared to the cosmetics containing chemical moisturizers (Comparative Formulation Examples 1 and 2) as a comparative formulation, the mixed extract of prickly pear fruit, Western cypress leaf, and Lilium candidum scale stem of the present invention It was confirmed that the moisturizing effect of the containing cosmetics (Formulation Examples 1 to 6) was superior. In addition, when comparing Formulation Example 1 and Formulation Examples 4 to 6, it was confirmed that since Formulation Example 1 had excellent efficacy, it was most preferable to mix each component at a mixing ratio of 1:1:1.

Experimental Example 2 : Skin barrier improvement effect

The study was conducted on 25 healthy women indoors at 25°C, relative humidity of 45%, and no air flow. Skin barrier damage was induced using a 1% aqueous solution of sodium lauryl sulfate (SLS), and the cosmetic compositions of Formulation Examples 1, 2, and 3 were applied to the upper arm and then treated with TEWAMETER TM210 (C+K eletronic). GmhH. Germany) was used to measure transepidermal water loss. In other words, the skin barrier recovery rate (%) was confirmed by measuring the change in TEWL (Transepidermal Water Loss) value over time. The experimental results are shown in Table 3 below.

division Skin barrier recovery rate (%) 2D 4D 6D 8D Formulation Example 1 62.78 81.98 92.22 98.45 Formulation Example 2 61.16 67.96 89.38 92.55 Formulation Example 3 58.82 67.45 83.73 85.06 Formulation Example 4 60.23 65.22 69.88 77.56 Formulation Example 5 57.52 58.88 71.23 76.14 Formulation Example 6 69.43 69.10 69.22 72.98 Comparative formulation example 1 30.97 41.23 45.64 40.78 Comparative formulation example 2 41.55 50.62 62.74 61.74

As shown in Table 3, Formulation Examples 1 to 6 containing mixed extracts of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stems do not contain prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stems. It can be seen that the skin barrier recovery rate was improved compared to Comparative Formulation Examples 1 and 2. In addition, when comparing Formulation Example 1 and Formulation Examples 4 to 6, it was confirmed that Formulation Example 1 had excellent efficacy and that mixing at a mixing ratio of 1:1:1 was most preferable.

Experimental Example 3 : Safety test

A human skin patch test was conducted to determine whether the cosmetic compositions of Formulation Examples 1 to 6 irritated the skin. The test was conducted on 20 women in their 20s and 30s who had never shown hypersensitivity to skin irritation in the past and did not currently have any symptoms of skin disease or skin allergy. The mixed extract of prickly pear fruit, cypress leaves, and lily madonna stems was used. Comparative formulation examples 1 and 2 that did not contain were used as control examples.

First, the test area was wiped with 70% ethanol and dried. The prepared test substance was dropped into a Finn chamber (100 The patch was applied for 24 hours, and the presence or absence of erythema and edema in the test area was observed using a magnifying glass (8MC-150, DAZOR) 1 hour and 24 hours after the patch was removed.

Skin reaction was determined according to the International Contact Dermatitis Research Group (ICDRG) regulations (Table 4), and the average skin reactivity was calculated using Equation 1 below and the results are shown in Table 5.

sign score Evaluation standard - 0 No response ± 0.5 faint or mild erythema + One Erythema, edema, and papules have clear boundaries but are mild. ++ 2 Marked erythema, papules, and vesicles +++ 3 Severe erythema, blisters, and crust formation

division 24 hours 48 hours Average skin response
(n=20) ± + ++ ± + ++ Formulation Example 1 One - - - - - 0.11 Formulation Example 2 - - - - - - 0.00 Formulation Example 3 One - - - - - 0.14 Formulation Example 4 One - - One - - 0.00 Formulation Example 5 2 - - - - - 0.21 Formulation Example 6 2 - - - - - 0.12 Comparative formulation example 1 2 - - - - - 0.28 Comparative formulation example 2 One - - One - - 0.29

As shown in Table 5, none of the cosmetic compositions of Formulation Examples 1 to 6 cause skin irritation, so the safety of the cosmetic formulation containing mixed extracts of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem is above. It was found that there was no such thing.

As the specific parts of the present invention have been described in detail above, it is clear to those skilled in the art that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. Accordingly, the practical scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

A skin moisturizing cosmetic composition containing mixed extracts of Opuntia Tuna Fruit, Thuja Occidentalis Leaf, and Lilium Candidum Bulb as active ingredients. The cosmetic composition for moisturizing skin according to claim 1, wherein the cosmetic composition has an effect of improving skin barrier function. The cosmetic composition for skin moisturizing according to claim 1, wherein the mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. The method of claim 1, wherein the mixed extract of prickly pear fruit, Western arborvitae leaves, and Lilium candidum scale stem is (a) water, anhydrous or hydrous lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, A solvent extraction method using an extraction solvent selected from the group consisting of acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, nucleic acid, and mixtures thereof, (b) supercritical extraction, and (c) ultrasonic extraction. A cosmetic composition for moisturizing skin, extracted by a method selected from . The cosmetic composition for skin moisturizing according to claim 1, wherein the prickly pear fruit, Western arborvitae leaves, and Lilium Madonna scale stem are contained in a mixed weight ratio of 1 to 3:1 to 3:1 to 3.