JPH09328410A - Cosmetic and its production - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cosmetic which is excellent in antimicrobial properties, ultraviolet absorption, prevents skin roughening and malting and keeps skin beautiful and provide its production process. SOLUTION: This cosmetic contains yucca extract and saponin, flavone and resveratrol are contained in the yucca extract. If the cosmetic is calculated as 100wt.%, the content of saponin, flavone and resveratrol is 0.001-5wt.%. The objective cosmetic is produced by chipping at least one of the roots or stems of yucca and drying, or drying and chipping them, crushing them into a powder, then soaking the powder in an ethanol-water mixed solvent thereby preparing the yucca extract. Subsequently, the yucca extract is formulated to a cosmetic. The ethanol-water mixed solvent contains 75-95wt.% of ethanol.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic and a method for producing the same, and more specifically, a cosmetic having excellent antibacterial properties and ultraviolet absorptivity, preventing rough skin and matting, and keeping beautiful skin, and a method for producing the same. Regarding

[0002]

2. Description of the Related Art Conventional cosmetics have been mixed with drugs for the purpose of various medicinal effects, but many of them have side effects even if the minimum amount of the medicinal effects is added. As a countermeasure, natural flora and fauna, especially plant extracts are blended (for example, loquat extract disclosed in Japanese Patent Publication No. 5-17206) and the like, but excellent in antibacterial properties and ultraviolet absorption properties. Has not yet been found.

[0003]

SUMMARY OF THE INVENTION The present invention solves the above-mentioned problems of the prior art, is excellent in antibacterial properties and ultraviolet absorptivity, etc., prevents rough skin and matte, and keeps beautiful skin. It is an object to provide a manufacturing method thereof.

[0004]

[Means for Solving the Problems] In order to achieve the above object, the present inventor has conducted extensive studies on the characteristics of the components of plant extracts, and as a result, the saponins contained in the Yucca extract have antibacterial properties. It has excellent antibacterial properties against yeast, and polyphenols contained in the above yucca extract, especially resveratrol (3,4 ′, 5-trihydroxystilbene), inhibit the life of the enzyme. In addition, saponin, flavone and resveratrol contained in the yucca extract are all excellent in ultraviolet absorptivity, and saponin, flavone and resveratrol are blended in cosmetics to make the skin rough and matte. The inventors have completed the present invention by discovering that a cosmetic that prevents the above-mentioned problems and keeps beautiful skin can be obtained.

According to the present invention, the present inventors have found that a cosmetic containing a yucca extract is excellent in preventing rough skin and preventing aging, and that this extract contains saponin, flavone and resveratrol. It was completed by finding out.

[0006] That is, the cosmetic of the first invention is a yucca (Y
ucca) extract. The above-mentioned "Yucca" is Yucca
schidigera), Atsuba Kimigayoran (Yuc)
ca gloriosa Linn, Yumiga recurvifolia Salis
b), Itlan (Yucca smallliana F
ernald), Senjuran (Yucca aloi)
folia Linn) or the like can be used. Also, one of these may be used, or two or more of these may be used. The "Yucca extract" may be the extract itself obtained by extracting from the raw material Yucca with a predetermined extraction solvent, or may be a concentrate obtained by concentrating this extract,
It may be a solid product obtained by drying these (powder, fruit granules, etc. may have any shape).

The yucca extract may contain saponin, flavone and resveratrol. The saponin, the flavone, or the resveratrol may be a yucca extract containing this component, or may be fractionated from the extract. Further, these may be mixed, or the inclusions of a plurality of components may be mixed.

The content of the saponin, flavone and resveratrol is 10% as shown in the third invention.
When it is 0% by weight, it can be 0.001 to 5% by weight, preferably 0.01 to 1% by weight. The content of the material is not sufficiently exhibited the effect of the substance is less than 0.001 wt%, the content is more than 5 wt%, colored or because the defects such as fine odor appears. Also,
When the above-mentioned three components of saponin, flavone and resveratrol are contained, when the amount of saponin is 10 parts by weight, flavone is 3 to 5 parts by weight and resveratrol is 0.
It is preferably 5 to 2 parts by weight.

In the method for producing a cosmetic of the present invention, at least one of the roots and stems of Yucca is made into chips and dried, or after dried and made into chips, it is pulverized to produce powder. Then, the powder is immersed in a mixed solvent of ethanol and water for extraction to obtain a yucca extract, and then the yucca extract is blended. The above "Yucca" has the same meaning as described above. And
As the yucca raw material, a root or a stem can be usually used, but both are usually used. The above ethano
The mixed solvent of water and water may have a mixing ratio of 75 to 95% by weight of ethanol as shown in the fifth invention. When the ethanol content is less than 75% by weight, the extract component contains a large amount of sugar and the ratio of the saponin component is low, and it takes too much time to extract yucca, which is an impossible rate.
This is because the content of the saponin component is reduced when the amount of syrup exceeds 95% by weight. Further, the form of the above-mentioned “Yucca extract” may be an extract, a concentrate or a solid as described above.

The form of the cosmetic of the present invention and the cosmetic produced by the production method of the present invention is not particularly limited, and examples thereof include a liquid (lotion, emulsion, etc.), cream or ointment. can do. If necessary, various additives generally used in cosmetics, for example, lower alcohols, aqueous components such as polyhydric alcohols, powder components such as titanium oxide and borax, oils, and surfactants. , Thickeners, antioxidants, fragrances, wrapping agents, drugs and the like can be added.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples. The Yucca extract was prepared, the cosmetics were prepared, and the performance thereof was evaluated by the following methods. (1) Preparation of Yucca extract (Part 1) Yucca schid
(igera) roots and stems were made into chips, which were dried in the sun and ground to obtain bulk powders. After dipping 500 g of this bulk powder in 5 times the amount of 90% ethanol and leaving it at room temperature for 24 hours,
It was filtered to obtain a 90% ethanol extract. This 90% ethanol extract was heated at atmospheric pressure to remove ethanol to obtain a concentrated solution containing 30 to 40% by weight of the active ingredient. Five times the amount of distilled water was added to these concentrated solutions and the mixture was left for 12 hours. Then, the upper layer aqueous solution and the lower layer water-insoluble matter were separated by decantation, and after the remaining amount became small, it was filtered. This aqueous solution is heated under reduced pressure to distill off the water and concentrate the water.
When the content reached about 0%, distillation was stopped, and 35 g of a brown powder (referred to as "Yucca extract 1") was obtained by vacuum, freezing or spray drying. The main components of this powder are saponin (60-70% by weight in dry powder) and sugar (30 in dry powder).
˜40% by weight). The weight ratio of saponin and sugar is about 2: 1. Then, after filtering off the water-insoluble matter,
It was dried under vacuum to obtain 15 g of a brown powder (referred to as "Yucca extract 1A"). The main components of this powder are flavone (75 to 85% by weight in dry powder) and resveratrol (15 to 25% by weight in dry powder). The weight ratio of this flavone to resveratrol is about 4: 1.

(Part 2) Active ingredient 30 obtained in Part 1
200 g of concentrated solution of -40% is developed sequentially with water, 30% ethanol and 80% ethanol using a column packed with ion exchange resin (trade name "Diaion HP20" manufactured by Mitsubishi Chemical Co., Ltd.). And fractionated. Water and 30% ethanol extract mainly contain sugar component, and 80% ethanol extract mainly contain saponin, flavone and resveratrol. This 80% ethanol extract was distilled under normal pressure in the initial stage and under reduced pressure in the latter half to distill off ethanol,
A concentrated solution containing 40 to 50% of active ingredient was obtained. Further, the concentrated liquid was vacuum dried to obtain 35 g of a brown powder (referred to as "Yucca extract 2"). The main components of this powder are saponin (87-90% by weight in dry powder), flavone (10-8% by weight in dry powder) and resveratrol (3-2% by weight in dry powder). The weight ratio of saponin, flavone and resveratrol is 10: 4: 1.

(Part 3) Active ingredient 30 obtained in Part 1
Two to three times the amount of distilled water was added to 100 g of a concentrated solution of -40% and left overnight at room temperature, and then the aqueous solution portion was separated by decantation. Using the same column as above, this aqueous solution was washed with water for the first time and then extracted with a 60% water-mixed solution of acetone for fractionation. The acetone solution was subjected to vacuum distillation, concentrated, allowed to cool, then frozen and dried to obtain 27 g of a powder (referred to as "Yucca extract 3"). The main component of this powder is saponin (90-100% by weight in dry powder).

(2) Preparation of Cosmetics The amount of cosmetics (facial lotion) (composition) in Example 1 is expressed in parts by weight (hereinafter referred to as “parts”. 1) Glycerin 1.0 parts 2) 1,3-butylene glycol 3.0 parts 3) Polyoxyethylene (15 mol addition) oleyl 0.3 parts Alcohol ether 4) Yucca extract 1; (Saponin : Sugar = 2: 1) 0.02 parts 5) Sodium citrate 0.1 parts 6) Ethanol 8.0 parts 7) Perfume 0.03 parts 8) Purified water 87.55 parts Total 100.0 parts

(Preparation) Glycerin, 1,3-butylene glycol and sodium citrate are added to purified water and dissolved by stirring. Separately, Yucca extract 1, polyoxyethylene oleyl ether and fragrance were dissolved in ethanol,
This was added to the above purified water aqueous solution, mixed uniformly, and then filtered to obtain a lotion.

Cosmetic (Toilet lotion) of Comparative Example 1 A lotion was obtained with the same composition and preparation as in Example 1 except that Yucca Extract 1 was not added.

Cosmetic (milky lotion) of Example 2 (Composition) 1) Refined jojoba oil 2.0 parts 2) Stearic acid 1.5 parts 3) Cetyl alcohol 0.3 parts 4) Polyoxyethylene (8 mol addition) ) Monooleic acid 1.0 part Ester 5) Sorbitan monostearate 6.5 parts 6) Yucca extract 2; 0.05 parts (saponin: flavone: resveratrol = 10: 4: 1) 7) Propylene Glycol 3.0 parts 8) Ethanol 3.0 parts 9) Perfume 0.01 parts 10) Purified water 82.64 parts Total 100.0 parts

(Preparation) Yucca extract 2 and fragrance are dissolved in ethanol to obtain an ethanol solution. Purified aqueous solution obtained by adding propylene glycol to purified water is 70-80.
Keep at ° C. The other components are mixed and dissolved by heating, the resulting solution is kept at 70 to 80 ° C., added to the above purified aqueous solution with stirring and emulsified, and after homogenized, further stirred with a homomixer, Add the above ethanol solution to finish. Further, this liquid was cooled to 30 ° C. by a heat exchanger (on-reader with a mixing and kneading function) to obtain an emulsion.

Cosmetics (cream) of Example 3 (Composition) 1) Refined jojoba oil 10.0 parts 2) Squalane 35.0 parts 3) Stearyl alcohol 3.0 parts 4) Polyoxyethylene (15 7. mol addition) palmitic acid 3.0 parts ester 5) sorbitan monostearate 1.0 part 6) yucca extract 1; (saponin: sugar = 2: 1) 0.1 part 7) propylene glycol 8. 0 parts 8) Fragrance 0.01 parts 9) Purified water 39.89 parts Total 100.0 parts

(Preparation) The raw materials 1) to 5) are heated to 70 to 80 ° C.
The raw material 6) which was heated to 75 ° C. and melted and heated to 75 ° C.,
Add to the aqueous solutions of 7) and 9 with stirring and pre-emulsify. Then, the raw material 8) was added and stirred with a homomixer to make the emulsified particles finer and uniform, and then rapidly cooled to 30 ° C. to obtain a cream.

Cosmetic (Ointment) of Example 4 (Composition) 1) Yucca extract 3 0.5 parts 2) Stearyl alcohol 15.0 parts 3) Beeswax 20.0 parts 4) Polyoxyethylene (5) Mol addition) sorbitan mono 0.3 part stearate 5) Polyoxyethylene (15 mol addition) monooleic acid 0.2 part ester 6) Vaseline 35.0 parts 7) Glycerin 5.0 parts 8) Purified water 24. 0 copies 100.0 copies

(Preparation) Raw materials 1) to 7) were dissolved by heating at 75 ° C. and added to purified water kept at 70 ° C., emulsified with a homomixer and then cooled to obtain an ointment.

(3) Evaluation of Performance for Improving Skin Roughness In order to clarify the performance of the above cosmetics, the following performance tests were conducted. Using the lotion obtained in Example 1 and the lotion obtained in Comparative Example 1, an effect of improving rough skin was tested on a human body panel. That is, a replica of the skin surface morphology of the face of a healthy female was taken using the replica method using myricin resin and observed under a microscope (17 times). Twenty-five people (rough skin panel) judged to be rough skin evaluations 1 and 2 according to the criteria shown in Table 1 from the condition of the skin pattern and the peeling state of the cornea were used, and the above lotions were applied to the left and right half of the face once a day. It was applied for 2 weeks. Two
After a week, the skin surface morphology of the face was observed again by the above replica method, and evaluated according to the criteria shown in Table 1. Table 2 shows the results.

[0024]

[Table 1]

[0025]

[Table 2]

The following can be seen from Table 2. That is, in the case of the face using the lotion containing Yucca extract 1 (Example 1), “0 skin groove, disappearance of cuticle, large area of horny horny layer” was 0, “skin groove, cuticle” Is unclear and the horny layer is turned up ", 0 was" Clear crevices and ridges "and 12 were" Clear creases and ridges ", while Yucca In the case of a face using a lotion containing no extract 1 (Comparative Example 1), 10 people said “disappearance of skin crevices, cuticles and large horny layer of horny layer”, “skin creases, cuticles are unclear, The number of "corneal creases" was 8 and the number of "skin and crust is clear" was 0. The number of "skin and crust was clear and regular" was 0. Therefore, the lotion containing the yucca extract 1 is more effective in preventing rough skin and matting and maintaining beautiful skin than the lotion containing no yucca extract 1.

(4) Evaluation of UV Absorption Performance The UV absorption performance of saponin, flavone and resveratrol is shown in FIG. 1 (saponin), FIG. 2 (flavone and resveratrol) and FIG. 3 (resveratrol). From these results, it can be seen that all the substances have excellent ultraviolet absorbing performance. Especially, saponin is 260-340
In terms of nm, flavone shows excellent UV absorption performance at about 260 to 320 nm and resveratrol at 280 to 340 nm. Therefore, it can be seen that if it contains saponin and / or flavone and resveratrol, it exhibits ultraviolet absorption performance in a wide range. The resveratrol used in this test was an organic synthetic product (purity 9
8% by weight) was used.

(5) Evaluation of whitening effect of resveratrol In order to see the whitening effect of resveratrol, the effect of resveratrol on B16 melanoma cells capable of producing melanin was examined. The resveratrol used in this test was an organic synthetic product (purity: 98% by weight). (Test Method) 1) Cell Culture The B16 melanoma cell solution was transferred to a culture flask 25 cm ² , 4 ml of a culture solution (DMEM containing 10% FBS) was added, and the conditions were 37 ° C., 5% -CO ₂ /95% -air. Cultured under. When the cells become semi-confluent, collect the cells by trypsinization, 5 × 10 ⁴ cells / 1
The cells were seeded on a 96-well multiplate so that the volume became 00 μl, and cultured again under the conditions of 37 ° C., 5% -CO ₂ /95% -air for 24 hours.

2) Measurement of tyrosinase biosynthesis inhibitory action After culturing in 96 well multiplate for 24 hours, the culture solution was removed, and 195 μl of a new culture solution and 5 μl of a sample solution of each concentration were added, and the mixture was incubated at 37 ° C. for 5%. -CO _2/95% -ai
It was cultured for 24 hours under the condition of r. After 24 hours, the culture solution containing the sample was removed, washed with PBS, and 1% -Triton X-
Add 50 μl of 100 / PBS solution, stir for 30 minutes,
Add 100 μl of 10 mM-DOPA / PBS solution and add 3
Incubation was carried out for 3 hours under the conditions of 7 ° C., 5% -CO ₂ /95% -air, and the absorbance was measured at a wavelength of 475 nm. Tyrosinase biosynthesis inhibition rate (%) = [(AB) / A]
× 100 A: Absorbance without sample addition B: Absorbance with sample addition

3) Measurement of cell viability After culturing in a 96-well multiplate for 24 hours, the culture solution was removed, 195 μl of a new culture solution and 5 μl of a sample solution of each concentration were added, and 37 ° C., 5% -CO ₂ / CO ₂ / 95% -ai
It was cultured for 24 hours under the condition of r. After 24 hours, the culture solution containing the sample was removed, washed with PBS, and the MTT-containing medium 10 was added.
0 μl was added and incubated for 4 hours. Four hours later,
The culture solution was removed, 200 μl of isopropyl alcohol was added, the mixture was allowed to stand for 30 minutes, and the absorbance was measured at wavelengths of 570 nm and 655 nm. Cell viability = [Y / X] × 100 X; value of absorbance without sample addition (570 nm-655 nm) Y; absorbance with sample addition (570 nm-655)
The value of the (nm) value was 50% ethanol aqueous solution.

(Test Results) Table 3 shows the test results. The following can be seen from Table 3. That is, resveratro
At a concentration of 10 ppm, a 51.4% inhibition rate of tyrosinase biosynthesis was observed, and a cell survival rate of 10%.
No cytotoxicity was observed at 0.3%. It is 5
Even in ppm, no cytotoxicity was observed, and the suppression rate was relatively excellent at 22.1%. On the other hand, when it was 50 ppm or more, it showed an excellent inhibition rate and a certain degree of cytotoxicity at a cell survival rate of 32.3%. Further, when it is 100 ppm or more, the cell viability is as low as 8.4%, and thus cytotoxicity is strongly recognized, and when it is 1 ppm or less, the inhibition rate is as low as 1.2% or less, which is excellent whitening. There was no effect. From the above, the concentration is 5 to 50 ppm
, The cytotoxicity was suppressed to a certain extent or more and the whitening effect was excellent to a certain extent or more. Of these, particularly at 5 to 10 ppm, no cytotoxicity was observed, and a certain degree of whitening effect was exhibited. Furthermore, from the above results, the concentration is 3-4.
According to 0 ppm, it is possible to obtain an excellent balance between the tyrosinase biosynthesis inhibitory effect and the cytotoxicity reducing property.

[0032]

[Table 3]

(6) Comprehensive Evaluation The effects of adding saponin, flavone and resveratrol contained in the yucca extract to cosmetics will be summarized. The cosmetic containing almost all of the above saponin has a rough skin improving effect and an ultraviolet absorbing effect. That is, saponin is effective in cosmetics. The above resveratrol alone has a remarkable whitening effect at a low concentration, but since it is very difficult to adjust this concentration, it is better to use resveratrol in combination with saponin or flavone than to use it alone. It has the effect of being used as a cosmetic. Flavone has an ultraviolet absorbing effect. Therefore, it is considered that flavone is also effective in cosmetics.

(7) Extraction conditions When the bulk powder obtained from Yucca was extracted with 80% ethanol,
Since saponin is dissolved in 80% ethanol, a highly concentrated extract of saponin can be obtained. Therefore, this is applicable when saponin is contained as the main component. From these results, similar results can be obtained with 100% ethanol. When the above-mentioned bulk powder is dipped in 90% ethanol, an extract containing three components of saponin, flavone and resveratrol can be obtained. Therefore, this can be applied when it is desired to prepare a cosmetic containing the above three components. 40% because the above 3 components are not extracted with water alone and 30% ethanol.
The above ethanol is considered necessary. Therefore, the above 3
In order to obtain an extract containing the components, 40 to 90% ethanol, preferably 50 to 90% ethanol, more preferably 70 to 90% ethanol can be applied.

The present invention is not limited to the specific examples described above, and various modifications may be made within the scope of the present invention according to the purpose and application. That is, one of a root and a stem may be used as the extraction raw material.
Further, in the above example, the crushed product obtained from the chips is used for extraction, but the extraction is not limited to this, and the chips may be used as they are, or the roots and / or stems may be directly crushed products. This pulverized powder may be used, and the size of the pulverized powder is not particularly limited. The extraction conditions, in particular, the type and extraction time of the extraction solvent, the temperature thereof, etc. can be changed variously. Further, the blending ratio of saponin, flavone and resveratrol obtained by fractionating (separating) from the yucca extract may be appropriately changed depending on the purpose. Furthermore, saponin obtained by fractionation (fractionation) from this yucca extract,
One or two of flavone and resveratrol may be blended in an appropriate amount, or depending on the extraction conditions without fractionation,
An extract (whether liquid or dry solid) contained by extracting one or two of these may be used.

[0036]

The cosmetics of the present invention and the cosmetics obtained by the method for producing the same are saponin, flavone and resveratro-
Since it contains syrup, it has excellent antibacterial properties and ultraviolet absorption properties, and has the effect of preventing rough skin and matting, and maintaining beautiful skin.

[Brief description of drawings]

FIG. 1 is a graph showing the ultraviolet absorption performance of Yucca extract 3 obtained in an example.

FIG. 2 is a graph showing the ultraviolet absorption performance of Yucca extract 1A obtained in the examples.

FIG. 3 is a graph showing the ultraviolet absorption performance of resveratrol.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI Technical display location A61K 35/78 ADA A61K 35/78 ADA ADZ ADZ AED AEDC C09K 3/00 104 C09K 3/00 104Z / / C12N 9/99 C12N 9/99

Claims (5)

[Claims] 1. A cosmetic composition comprising a Yucca extract. 2. The cosmetic according to claim 1, wherein the yucca extract contains saponin, flavone and resveratrol. 3. The cosmetic according to claim 2, wherein the content of the saponin, flavone and resveratrol is 0.001 to 5% by weight when the amount of the cosmetic is 100% by weight. 4. At least one of the roots and stems of Yucca is made into chips and dried, or after being dried and made into chips, it is pulverized to produce a powder, and then the powder is mixed with ethanol. A method for producing a cosmetic, which comprises immersing in a mixed solvent of water to extract a yucca extract, and then blending the yucca extract. 5. The mixed solvent of ethanol and water has a mixing ratio of 75 to 95% by weight of ethanol.
A method for producing the described cosmetic.