JPH05124930A - Cosmetic - Google Patents

Abstract

PURPOSE:To provide a cosmetic containing the extract of the root, subterranean stem or leaf of bamboo, having excellent moisture-retention and cell-activating effect, exhibiting extremely excellent effect on the prevention and amelioration of dry skin and chapped skin and having hair-growth promoting effect as a hair-tonic. CONSTITUTION:The objective cosmetic contains the extract of root, subterranean stem or leaf of bamboo in an amount of preferably 0.1-5% in terms of dried solid component based on the total cosmetic. The extract can be produced preferably by immersing the root, subterranean stem or leaf of Phyllostachys bambusoides in a hydrophilic organic solvent such as methanol and subjecting the extract to filtration and vacuum drying. The base for the cosmetic is e.g. purified water or alcohol.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic for skin or hair which is excellent in moisturizing effect and cell activating effect.

[0002]

2. Description of the Related Art Conventionally, it has been known to add various moisturizers and oils to cosmetics in order to maintain the moisturizing function of the skin and improve dry and aged skin. Conventional moisturizers such as glycerin and propylene glycol are formulated with the expectation of an effect of indirectly improving rough skin by preventing moisture evaporation on the skin surface, but the effect is temporary. However, it was not intended to improve the rough skin and to make the physiological function of the skin healthy.

[0003]

DISCLOSURE OF INVENTION Problems to be Solved by the Invention As a result of intensive studies conducted by the present inventors in view of the fact that the effects of these conventional moisturizing cosmetics are not always sufficient, as a result, bamboo which has never been neglected as a cosmetic composition has been obtained. It was found that the root extract, rhizome or leaf extract (hereinafter sometimes simply referred to as a bamboo extract) has an extremely effective cell growth promoting action in addition to an excellent moisturizing effect.

The present invention has been made on the basis of the newly discovered facts, and it is an object of the present invention to provide a cosmetic composition exhibiting a rough skin improving effect which is far superior to conventional moisturizing cosmetic compositions.

[0005]

The object of the present invention is as follows.
This can be achieved by incorporating a bamboo extract into a cosmetic and allowing it to be contained. That is, the present invention is a bamboo root,
A cosmetic comprising an extract of rhizome or leaf.

[0006] In order to obtain a bamboo extract to be incorporated in the cosmetic of the present invention, as a raw material bamboo, Phellistosty
s bambusoides Sieb et Zucc.), Hachiku (Phyllostachy)
s ni-gra MUNRO var.henonis STAPF), Moso bamboo
(Phyllostachys pubescens) is used. Then, after extracting the roots, rhizomes or leaves of these bamboos with one or a mixture of two or more hydrophilic organic solvents, or with a water-containing organic solvent containing 30% by volume or more of these by cold or hot digestion, The bamboo extract is obtained by filtration and drying under reduced pressure.

As the hydrophilic organic solvent used for such extraction, those selected from lower alcohols such as methanol, ethanol, n-propanol and i-propanol, and lower ketones such as acetone and methyl ethyl ketone are preferably used. it can.

The content of the bamboo extract in the cosmetic of the present invention is 0.001 to the total amount of the cosmetic as a dry solid.
It is 10%, preferably 0.1-5%. If the blending amount is less than 0.001%, a sufficient effect cannot be obtained, and 1
Even if it exceeds 0%, the effect cannot be expected to increase.

The cosmetic of the present invention can be in the form of, for example, lotion, emulsion, cream, pack, shampoo, rinse, hair tonic and the like. In the cosmetic of the present invention, for example, purified water, alcohols and the like can be used as a base material, but other components such as oils and fats and surface active agents may be added as necessary within a range that does not impair the effects of the present invention. Agents, polyhydric alcohols, acidic mucopolysaccharides, collagen, amino acids, herbal medicine extracts and the like can be appropriately added.

[Reference Example 1] 5 kg of rhizomes and roots of Pleurotus cornucopiae were chopped, 40 L of 90% ethanol was added thereto, gently heated for extraction, and then filtered. The residue was repeatedly extracted with 40 L of 90% ethanol in the same manner, the extracts were combined 5 times, the solvent was distilled off under reduced pressure, and the residue was evaporated to dryness to obtain bamboo extract A.

The cell growth promoting action of this bamboo extract A was measured by the following method. Cryopreserved juvenile hamster kidney cells (clone 21) were thawed and centrifuged to
ME containing 0% bovine serum and 2 mM L-glutamic acid
The dispersion in M medium was placed in a culture flask so that the number of cells was 2 × 10 ⁵ cells per 25 ml, and the cells were cultured at 37 ° C. in an air atmosphere containing 5% carbon dioxide.

After culturing for 3 days in this way, it was treated with a saline solution containing 0.25% trypsin, centrifuged and dispersed in a MEM medium containing 10% bovine serum. After counting the number of dispersed cells in the medium, the cell dispersion medium was added to the wells of the microplate so that the number of cells was about 1 × 10 ^4, and the cells were placed in an air atmosphere containing 5% carbon dioxide at 37 ° C. Two
Cultured for 4 hours.

After such pre-culture, the culture solution was removed, 10% bovine serum-containing MEM medium in which bamboo extract A was dissolved at different concentrations was added to each well, and the same procedure as above was performed. The cells were cultured under the conditions for 72 hours.

After completion of the test culture, the culture solution was removed, and each well had a pH 7.2 phosphate buffered saline solution (hereinafter referred to as PBS).
1 ml) and then treated with 0.2 ml of a saline solution containing 0.25% trypsin, followed by 10%
0.2 ml of PBS containing each bovine serum was added. Immediately thereafter, 0.4 ml of 0.5% trypan blue-containing saline was added to each well, the number of unstained live cells was counted on a hemocytometer, and the number of cells per well was determined.

The relationship between the concentration of bamboo extract A in 10% bovine serum-containing MEM medium and the number of cells was determined by such a method, and the cell increase / decrease rate was calculated. The results are shown in Table 1.
The concentration of bamboo extract A was expressed in mg / ml.

[0016]

[Table 1] Table 1 ─────────────────────────────── Concentration 0 6.25 12.5 25 50 100 (mg / ml) ─ ────────────────────────────── Average number of cells 2.6 3.2 3.6 3.6 3.4 3.4 (× 10 ⁴ ) Change rate 100 123 138 138 131 131 (%) Change 0 23 38 38 31 31 (%) ─────────────────────────────── These results It can be seen from the results that Bamboo extract A has a cell growth promoting action.

Reference Example 2 Root mushrooms, rhizomes and leaves 5 of Japanese bamboo shoots
The kg was cut into small pieces, and 40 L of 90% acetone was repeatedly extracted in the same manner as in Reference Example 1 to combine the extracts 5 times, the solvent was distilled off under reduced pressure, and the mixture was evaporated to dryness to obtain a bamboo extract. B
Got

Example 1 The skin milk of the present invention was produced according to the following formulation. Bamboo extract A 0.5 g squalane 10.0 g behenyl alcohol 1.5 g polyoxyethylene sorbitan monostearate 2.0 g sorbitan monostearate 1.0 g paraben 0.3 g Purified water is added to bring the total amount to 100 g.

Example 2 A skin cream of the present invention was produced according to the following formulation. Bamboo extract B 0.1 g Soybean phospholipid 2.0 g Squalane 4.0 g Behenyl alcohol 4.0 g Cholesteryl hydroxystearate 1.0 g Neopentyl glycol dioctanoate 5.0 g Isopropyl palmitate 5.0 g Paraben 0.3 g Glycerin 5.0 g Purified water added to bring the total amount to 100 g To do.

Example 3 A hair nourishing agent of the present invention was produced according to the following formulation. Bamboo extract B 1.0 g Cepharanthin 0.002 g Dicaprylic acid pyridoxine 0.1 g Acetate dl-α-tocopherol 0.02 g Cemblyx extract 2.0 g Carrot extract 1.0 g Ginger deer tincture 1.0 g Polyoxyethylene hydrogenated castor oil 0.8 g Ethanol 45.0 g Purified water Is added to bring the total amount to 100 g.

Example 4 A shampoo of the present invention was produced according to the following formulation. Bamboo extract A 0.5 g N-lauroyl-N-methyl-β-alanilate 10.5 g 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium 3.0 g Diethanolamine laurate 5.0 g Propylene glycol 3.0 g Paraben 0.3 g Perfume Add 0.2 g purified water to make 100 ml.

Comparative Example 1 A comparative skin milk was produced according to the following formulation. Glycerin 5.0 g Squalane 10.0 g Behenyl alcohol 1.5 g Polyoxyethylene sorbitan monostearate 2.0 g Sorbitan monostearate 1.0 g Paraben 0.3 g Purified water is added to bring the total amount to 100 g.

Comparative Example 2 A comparative hair nourishing agent was produced according to the following formulation. Cepharanthin 0.002 g Pyridoxine dicaprylate 0.4 g Acetate dl-α-tocopherol 0.2 g Senburi extract 2.0 g Carrot extract 1.0 g Ginger deer 1.0 g l-menthol 0.1 g Polyoxyethylene hydrogenated castor oil 0.8 g Ethanol 60.0 g Purified water In addition, the total amount is 100 ml.

[Test Example 1] A comparative test of the moisturizing effect was conducted using the skin milks of Example 1 and Comparative Example 1 by the following method.

Biological stratum corneum water load test After resting in a room at a temperature of 20 ° C. and a humidity of 40% for 10 minutes, a sample of 1.25 μL / cm ² was placed on the test site of 2 × 2 cm set on the flexion side of the forearm. Apply evenly with the coating density of. After left for 2 hours, the skin conductance meter (IB
The conductance value is measured by using SKICON-200 manufactured by S company. Next, place distilled water on the test site for 10
After a few seconds, the water droplets are completely wiped off with dry gauze, and the conductance value is measured immediately after and after 30, 60, 90 and 120 seconds. As a control, perform the same operation on the test site without sample application. In this test, the larger the conductance value, the better the moisturizing effect.

The results of the measurement are shown in FIG. 1. The results show that the bamboo extract contained 10% of glycerin, which is a typical moisturizer.
It can be seen that a moisturizing effect superior to that of glycerin is exhibited even with a compounding amount of one-half.

[Test Example 2] The skin cream of Example 2 was used to test the effect of improving rough skin by the following method.

Test for Improvement Effect on Rough Skin Five people, male and female, aged 20 to 50, who suffer from rough skin and dry skin were subjected to skin cream application for 2 weeks, and then the effect of improving rough skin was visually observed. The results are shown in Table 2.

[0029]

[Table 2] Table 2 ─────────────────────────── Subject A B C D E ──────────── ──────────────── Evaluation 〇 〇 △ △ 〇 ─────────────────────────── ◯: Improves △: Improves a little ×: Does not change

From these results, it can be seen that the cosmetic of the present invention of Example 2 has an excellent effect of improving rough skin.

[Test Example 3] A comparative test of the hair nourishing effect of the hair nourishing agent of Example 3 and the hair nourishing agent of Comparative Example 2 was conducted by the following method. The results of the test are shown in Table 3.

Hair- growth effect test : The dorsal part of 10 7-week-old male mice (C57BL / 6CrSIC) was shaved, applied with a creamy hair remover (Shiseido Co., Ltd.), left for 15 minutes, and then wiped off thoroughly with gauze. Wash with warm water. After the lapse of 24 hours, the hair loss site was divided into an upper part and a lower part, and for 5 animals, the present invention product was placed on the upper part, and on the lower part, the comparative product was placed on the upper part and on the lower part, the present invention product was placed on the lower part. Each of these was applied twice a day, and the condition of growing hair was observed every day. Then, after 12 days from the test start date, about 20 hairs are extracted from each individual test section, and 10 hairs are selected from the remaining long hairs except for extremely long hairs. The hair length (mm) was measured with a stereo microscope equipped with a meter, and the average value was calculated for each.

[0033]

[Table 3] Table 3 ────────────────────── Specimen Invention product Comparative product ───────────────── ────── Hair length 12th day 0.00 0.00 15th day 0.00 0.00 18th day 1.17 0.82 21st day 2.69 1.61 ────── ────────────────

From these results, it can be seen that the hair nourishing agent of the present invention of Example 3 exhibits a superior hair nourishing effect to the hair nourishing agent of Comparative Example 2.

[0035]

EFFECT OF THE INVENTION The cosmetic composition of the present invention exhibits an extremely excellent effect in preventing and improving dry skin and rough skin. Further, when used as a hair nourishing agent, it moisturizes the scalp by an excellent moisturizing effect and cell growth promoting effect and exerts a hair growth promoting effect.

[Brief description of drawings]

FIG. 1 is a graph showing the results of a moisturizing effect test conducted on skin milk.

Claims (5)

[Claims] 1. A cosmetic comprising an extract of bamboo root, rhizome or leaf. 2. The cosmetic according to claim 1, which is for moisturizing skin or promoting hair growth. 3. The cosmetic material according to claim 1, wherein the bamboo is edema, bees, or moss. 4. The cosmetic according to claim 1, wherein the extract is obtained by extracting the roots, rhizomes or leaves of bamboo with a hydrophilic organic solvent. 5. The cosmetic according to claim 4, wherein the hydrophilic organic solvent is selected from lower alcohols and lower ketones.