KR20180051107A - Cosmetic composition for skin moisturizing containing gynura procumbens extract and pandanus conoideus lam extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin moisturizing containing a Gynura procumbens extract and a Pandanus conoideus lam extract as active ingredients, and more specifically, to a cosmetic composition for skin moisturizing, which has excellent skin moisturizing effects and contains a mixture of a Gynura procumbens extract and a Pandanus conoideus lam extract as active ingredients.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition for moisturizing cosmetic composition,

The present invention relates to a process for the preparation of ( Pandanus conoideus Lam ) as an active ingredient. More particularly, the present invention relates to a cosmetic composition containing a procumbens extract and a Pandanus conoideus Lam as an active ingredient, To a cosmetic composition for protection.

The skin protects the body from various external stimuli and has a barrier function to prevent the inner moisture. In the epidermis of the skin, it develops as a thin stratum corneum containing water through differentiation process. Structurally, the cells in the stratum corneum act like bricks, and the intercellular lipids of the keratinocytes act as mortar to form the skin barrier.

However, the human body tends to increase in dry skin due to aging, and the need for a skin moisturizing agent is increasing due to air pollution and indoor heating. For this purpose, saccharides and polyhydric alcohols are used as a moisturizing agent as a general moisturizing agent in cosmetics However, these moisturizers have a disadvantage in that the skin becomes sticky, and there is a problem that the moisturizing effect and the continuous moisturizing effect are not given to the skin. In addition, in the summer when the humidity is very high, moisture is drawn from the outside and the skin is sticky. In winter, the humidity of the outside air is low, and rather, the moisture is drawn from the skin and the skin is dried.

Water acts as a carrier of the physiologically active substance required for the human body and allows the skin to maintain a smooth and moist condition. However, its functions can easily be lost due to environmental factors such as excessive washing, bathing, environmental factors such as dry atmosphere, pollutants, etc., and in recent years, due to the increase of various harmful factors, The number of people suffering from various disabilities is increasing.

Various cosmetic compositions have been tried to improve the dryness of the skin by using various moisturizing agents such as sugar, polyol and the like. For example, natural moisturizing factors in the stratum corneum, which suppress moisture evaporating from the skin or easily combine with water molecules on the skin surface to maintain moisture in the stratum corneum, by strengthening the skin barrier function using ceramide, cholesterol, (Hyaluronic Acid), sugars and polyols that have strong ability to bind to natural moisturizing factors (NMF), sodium pyrrolidonecarboxylate, lactate and water, .

Furthermore, in recent years, many cosmetics using natural products have been developed to reduce skin irritation caused by external chemicals and the like. Natural products are not only less harmful to the skin, but they are also increasingly being developed as cosmetics raw materials as consumers' demand for cosmetics using natural ingredients increases.

Accordingly, the present inventors have studied the applicability of various natural materials not yet known to cosmetics. As a result, procumbens and Pandanus conoideus Lam were selected and the mixed extracts were prepared and the moisturizing effect was measured. As a result, the efficacy of each extract was much better than that of using the extracts independently, .

SUMMARY OF THE INVENTION [ 0006] procumbens extract and Pandanus conoideus Lam extract to provide a cosmetic composition exhibiting a moisturizing effect.

It is also an object of the present invention to provide a cosmetic composition exhibiting an efficacy by preparing supernatant, ultrasound and fermentation extracts and purifying the extract to maximize the efficacy of Gynura procumbens and Pandanus conoideus Lam extracts have.

The present invention relates to a cosmetic composition for moisturizing, which contains a mixed extract of Namyeolcho extract and Buamera extract.

Preferably, the cosmetic composition has a skin barrier function recovery effect.

Preferably, the mixed extract of Namyeolcho extract and Buamera extract is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the combined extracts of Namyeolcho extract and Buamera extract are (a) water, an anhydrous or a lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl (B) a supercritical extraction method, (c) an ultrasonic extraction method, and (d) a fermentation extraction method using a microorganism, wherein the solvent extraction method comprises a solvent extraction method using an extraction solvent selected from the group consisting of acetone, diethyl ether, dichloromethane, Lt; / RTI >

Preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray ≪ / RTI >

The cosmetic composition containing the mixed extract of Gynura procumbens extract and Pandanus conoideus Lam extract according to the present invention is excellent in moisturizing effect (Experimental Example 1).

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The term "about" is used herein to refer to a reference quantity, a level, a value, a number, a frequency, a percent, a dimension, a size, a quantity, a weight, or a length of 30, 25, 20, 25, 10, 9, 8, 7, Level, value, number, frequency, percent, dimension, size, quantity, weight or length of a variable, such as 4, 3, 2 or 1%.

Throughout this specification, the words "comprises" and "comprising ", unless the context requires otherwise, include the steps or components, or groups of steps or elements, Steps, or groups of elements are not excluded.

Gynura procumbens , which is used as an active ingredient in the cosmetic composition of the present invention, is also called Sambung Nyawa, because it is Indonesian, Sambung is permanent, and Nyawa is life. Thus, It is a medicinal plant with the name "Life Sustainable Plant" (English name). It is used as a traditional herb in China. According to Chinese and Southeast Asian traditional medicine, it is known to be effective for cough, bronchitis, pulmonary tuberculosis, blood circulation and bruises. It is used for folk medicine and food in Southeast Asia and Indonesia. It is also known to have an effect of preventing hypertension, diabetes, cholesterol, kidney disease and improving symptoms. The term "herbal extract" of the present invention means obtained from various organs or parts (eg, leaves, roots, stems, etc.) of the beginning of menstrual period, preferably from leaves.

Pandanus conoideus Lam , which is used as an active ingredient in the cosmetic composition of the present invention, is used in a family (Pandanaceae), which is mainly distributed in the Jayawijay Mountain alpine region near the city of Indonesia and Wamena City, Buah Merah, Red Fruit, etc., and its scientific name is Pandanus conoideus Lam . Buamera contains beta-carotene, omega-3 and omega-9 as well as various minerals and is known to be effective for anti-inflammatory, arthritis, rheumatism and spondylitis. In the present invention, the bumamera extract is obtained by extracting from various organs or parts of bumamera.

In addition, the Herbal Extract and Biala extract of the present invention can be prepared according to a conventional method known in the art. For example, extraction is performed using (a) a solvent extraction method, (b) a supercritical extraction method using reduced pressure by carbon dioxide, or (c) an ultrasonic extraction method.

In the present invention, the extracts of Bombyx mori extract and Buamera extract are obtained by extracting various extracting solvents such as water, anhydrous or hydrated lower alcohol (for example, methanol, ethanol, propanol and butanol) having 1 to 4 carbon atoms, propylene glycol, Can be obtained by using at least one extraction solvent selected from the group consisting of 3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, nucleic acids and mixtures thereof, Is obtained by using ethanol, 70% (v / v) ethanol or water, and most preferably by using 70% (v / v) ethanol.

It will be apparent to those skilled in the art that the extracts of Namyeongcho and Buamera extracts of the present invention can be obtained not only by the above-mentioned extractants but also by other extractants having substantially the same effect.

In addition, the extract of Oriental herbal medicine and the extract of Buamera of the present invention include not only the extract obtained by the above-mentioned extraction solvent, but also the extract obtained through ordinary purification and fermentation process. For example, decompression by carbon dioxide, extraction by supercritical extraction at high temperature, extraction by extraction using ultrasound, separation by ultrafiltration membrane with constant molecular weight cut-off value, various chromatography (size, charge, hydrophobic or affinity And the active fraction obtained through various purification and extraction methods, such as those extracted by fermentation products using natural or various microorganisms, are also included in the extract of the present invention.

Further, the present invention provides a cosmetic composition characterized in that the above extract is obtained by liquid-freezing obtained by cold-pressing and heating filtration at room temperature, and further by concentrating or lyophilizing the solvent under reduced pressure.

The supercritical fluid extraction refers to supercritical fluid extraction. Generally, the supercritical fluid is extracted from the liquid and the liquid when the gas reaches the critical point at high temperature and high pressure. (J. Chromatogr. A. 1998; 479: 200-205). In addition, it has been reported that the supercritical fluid has a polarity similar to that of a nonpolar solvent.

Carbon dioxide is a supercritical fluid with both liquid and gaseous nature, with the operation of supercritical fluid equipment reaching its critical pressure and temperature, resulting in increased solubility in fat-soluble solutes. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the lipophilic substance contained in the sample is extracted into supercritical carbon dioxide.

When the supernatant carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction vessel after extracting the lipid-soluble substance, components that were not extracted by pure supercritical carbon dioxide can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract an active ingredient by using a mixed fluid in which a cosolvent is further mixed with supercritical carbon dioxide or carbon dioxide.

These co-solvents may be used alone or in admixture of two or more selected from the group consisting of chloroform, olive oil, ethanol, methanol, water, ethyl acetate, nucleic acid and diethyl ether. Preferably, one or a mixture of two or more selected from the group consisting of olive oil, ethanol, methanol, and water can be used.

Most of the extracted samples contain carbon dioxide. Since the carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the cosolvent can be removed by a reduced pressure evaporator.

In addition, the ultrasonic extraction method is an extraction method using energy generated by ultrasonic vibration. Ultrasonic waves can destroy an insoluble solvent contained in a sample in a water-soluble solvent. Due to the high local temperature generated at this time, Since the kinetic energy of the particles is increased, sufficient energy required for the reaction is obtained. By inducing the high pressure by the shock effect of the ultrasonic energy, the mixing efficiency of the substance contained in the sample and the solvent is increased, thereby increasing the extraction efficiency.

As the extraction solvent usable in the ultrasonic extraction method, one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, nucleic acid and diethyl ether can be used. The extracted sample is recovered by vacuum filtration, the filtrate is recovered, and the extract is removed by a vacuum evaporator and lyophilized to obtain an extract.

The herbal extract and the bumamera extract according to the present invention also include fermented extracts. The herbal extract and the bumamera extract can be prepared as follows. The microbial broth is first finely crushed to a size of about 100 to 500 mesh, then 1 to 50 g / L of a conventional microbial culture solution is added, and microorganisms such as yeast strains or lactic acid bacteria are added in an amount of 10,000 to 100,000 cfu / L. The culture temperature is 30 to 37 ° C. The pH is 5 to 7 and aerobic or anaerobic conditions are maintained for about 5 to 10 days. Which can be obtained through aging and filtration.

According to the present invention, the optimal mixing ratio of the Namhae early extract and the Buamera extract is 1: 10: 1 to 10: 1, more preferably 1: 1. On the other hand, the mixed extract of the present invention may be extracted after mixed with Namhaegi and Buamera, or may be mixed after the respective extracts are obtained.

The mixed extract of Namyeolcho extract and Buamera extract is contained in an amount of 0.001 to 30.0% by weight, preferably 0.01 to 20% by weight, more preferably 0.1 to 10% by weight, based on the total weight of the cosmetic composition. . When the content of the extract is less than 0.001% by weight, the skin moisturizing effect is not exhibited. When the content is more than 30.0% by weight, the skin moisturizing effect is insignificant to the increase of the content, It is not even.

A cosmetic composition that exhibits an efficacy after removing unnecessary components through a purification process in order to use the mixed extract of Myogusae mori extract and Buamera extract prepared by a conventional method in a cosmetic formulation can be included. The purification process can be any ordinary purification process, and a liquid-liquid extraction method using a difference in solubility, a chromatography method using a precipitation and a filter, and a polarity and a molecular size can be used. In addition, for example, after extracting Namseolcho extract and Buamera extract in ethanol aqueous solution (50%, 70% solution or the like) or other aqueous solution, HP-20 and sephadex columns were packed and the polarity and molecular weight difference , The water-soluble substance and the oil-soluble substance are separated, and the oil-soluble substance is concentrated and freeze-dried by TLC to prepare a concentrate.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, and may contain conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, Supplements, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powdered foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the cleansing containing the surfactant in the formulations of the present invention, it is preferable to use, as the carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, washed or rinsed with water. The surfactant-containing cleansing formulation may be a cleansing foam, a cleansing water, a cleansing towel and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Manufacturing example  One: The beginning of the month  Solvent extract

300 g of various organs or parts of the shrubs were collected by refluxing three times for 5 hours in a 70% (v / v) aqueous ethanol solution, cooled down, and filtered through Whatman # 4 filter paper. The filtered extracts were washed with ultrafiltration membrane and concentrated under reduced pressure at 50 ℃ or lower. Then, the dried powder extracted from the first half of May was dissolved in 50% 1,3-butylene glycol and used.

Manufacturing example  2: The beginning of the month Supercritical  Fluid extract

300 grams of various organs or parts of the shrubby shrubs early in the month are extracted using conventional supercritical extraction methods (using olive oil as co-solvent under supercritical conditions at a pressure of about 300 atm at a temperature of about 60 DEG C) After removing the salt by ultrafiltration membrane, the extract was concentrated under reduced pressure and lyophilized. This was dissolved in 50% 1,3-butylene glycol and used.

Manufacturing example  3: The beginning of the month  Ultrasonic Extract

300 g of various organs or parts of the shrubs were extracted using ultrasound (Extracted at 30 ° C for about 2 hours at 25 KHz using SuperNonic Ultrasonic apparatus), and the salts were removed using an ultrafiltration membrane The extract powder was obtained by concentration under reduced pressure and freeze drying. The dried powder extracted from the first month of May was dissolved in 50% 1,3-butylene glycol and used.

Production Example 4: Buamera solvent extract

300 g of the various organs or parts of the shaded bumamera were refluxed three times for 5 hours with 70% (v / v) ethanol aqueous solution, cooled down, and filtered through Whatman # 4 filter paper. The filtered extract was washed with ultrafiltration membrane, concentrated under reduced pressure at 50 ℃ or lower and freeze-dried to obtain bumamera extract powder. The dried bumela extract powder was then dissolved in 50% 1,3-butylene glycol and used.

Production Example 5: Buameracosus critical fluid extract

300 g of the various organs or parts of the shredded bumamera were extracted by conventional supercritical extraction (extraction with olive oil as co-solvent under supercritical conditions at a pressure of about 300 atmospheres (atm) at a temperature of about 60 DEG C) After removing the salt using ultrafiltration membrane, the mixture was concentrated under reduced pressure and lyophilized to obtain bumamera extract powder. This was dissolved in 50% 1,3-butylene glycol and used.

Manufacturing example  6: Buamera ultrasound extract

300 g of various organs or parts of shaded bumamera were extracted using ultrasound (extraction with a superannic ultrasonic device at 25 ° C for about 2 hours at 30 ° C), and the salts were removed using an ultrafiltration membrane Concentrated under reduced pressure and lyophilized to obtain bumamera extract powder. The dried bumela extract powder was dissolved in 50% 1,3-butylene glycol and used.

Formulation Example  Preparation of 1 to 9: The beginning of the month  Cosmetic composition containing an extract and a bougainum extract

Cosmetic preparations (Formulation Examples 1 to 9) comprising a mixture of an extract of Namyeokcho and an extract of Buamerra extract, which were prepared by mixing the Namyeokcho extract of Preparations 1 to 3 and the Buamerea extract of Preparations 4 to 6 at a weight ratio of 1: And the results are shown in Table 1 below.

ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 Formulation Example 7 Formulation Example 8 Formulation Example 9 Production Example 1 2.5 2.5 2.5 - - - - - - Production Example 2 - - - 2.5 2.5 2.5 - - - Production Example 3 - - - - - - 2.5 2.5 2.5 Production Example 4 2.5 - - 2.5 - - 2.5 - - Production Example 5 - 2.5 - - 2.5 - - 2.5 - Production Example 6 - - 2.5 - - 2.5 - - 2.5 glycerin - - - - - - - - - 1,3-butylene glycol - - - - - - - - - Sorbitol - - - - - - - - - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Cetostearyl alcohol 2 2 2 2 2 2 2 2 2 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystalline 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Squalane 5 5 5 5 5 5 5 5 5 Liquid paraffin 2 2 2 2 2 2 2 2 2 Trioctanoin 3 3 3 3 3 3 3 3 3 Polysorbate One One One One One One One One One Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl acetate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Cyclomethicone 4 4 4 4 4 4 4 4 4 BHT 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Comparative Formulation Example  Preparation of 1 to 9: The beginning of the month  A cosmetic composition containing an extract and a bougainum extract, respectively, and a cosmetic composition containing the same

(Comparative Formulation Examples 1 to 3), Preparation Examples 4 to 6 (Comparative Formulation Examples 4 to 6) containing the bumamera extract, Production methods of the extracts of Oriental herb medicine Cosmetic preparations (Comparative Formulation Examples 7 to 8) containing glycerin and 1,3-butylene glycol as moisturizers (Comparative Formulation Examples 7 to 8) and cosmetic compositions (Comparative Formulation Example 9) containing no extract or moisturizer were prepared as shown in Table 2 below .

ingredient Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Comparative Formulation Example 4 Comparative Formulation Example 5 Comparative Formulation Example 6 Comparative Formulation Example 7 Comparative Formulation Example 8 Comparative Formulation Example 9 Production Example 1 5 - - - - - - - - Production Example 2 - 5 - - - - - - - Production Example 3 - - 5 - - - - - - Production Example 4 - - - 5 - - - - - Production Example 5 - - - - 5 - - - - Production Example 6 - - - - - 5 - - - glycerin - - - - - - 5 5 - 1,3-butylene glycol - - - - - - 5 - - Sorbitol - - - - - - - 5 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Cetostearyl alcohol 2 2 2 2 2 2 2 2 2 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystalline 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Squalane 5 5 5 5 5 5 5 5 5 Liquid paraffin 2 2 2 2 2 2 2 2 2 Trioctanoin 3 3 3 3 3 3 3 3 3 Polysorbate One One One One One One One One One Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl acetate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Cyclomethicone 4 4 4 4 4 4 4 4 4 BHT 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Experimental Example  One: The beginning of the month  Moisturizing effect of extract and Buamera extract

The clinical moisturizing effect and the sustaining power for Formulation Examples 1 to 9 and Comparative Formulation Examples 1 to 9 were measured as follows. A total of 180 healthy male and female adults who felt skin dryness were randomly divided into groups of 10, and each of the formulations and the comparative formulations for each group were administered twice daily for four weeks, one for each side, Respectively. Moisturizing effect was evaluated by quantifying the increase rate (%) of skin conductivity according to the skin moisture content using the Corneometer CM 820 (Corage + Khazaka, Germany) for 2 weeks from the start of the test and after the completion of the test. The experimental results are shown in Table 3 below.

Increase rate of skin electrical conductivity (%) division Before use After 2 weeks of use After 4 weeks of use Formulation Example 1 25.23 43.22 51.54 Formulation Example 2 24.48 42.57 51.8 Formulation Example 3 23.63 43.88 51.88 Formulation Example 4 25.49 43.64 51.72 Formulation Example 5 24.72 42.99 50.73 Formulation Example 6 23.87 44.3 52.39 Formulation Example 7 25.02 42.93 51.96 Formulation Example 8 25.87 43.36 52.64 Formulation Example 9 23.85 42.25 50.74 Comparative Formulation Example 1 25.36 39.38 43.78 Comparative Formulation Example 2 24.61 37.8 42.72 Comparative Formulation Example 3 23.75 38 42.66 Comparative Formulation Example 4 25.62 39.78 43.77 Comparative Formulation Example 5 24.85 38.2 42.89 Comparative Formulation Example 6 23.99 38.41 43.15 Comparative Formulation Example 7 25.15 33.28 39.99 Comparative Formulation Example 8 26 34.64 38.74 Comparative Formulation Example 9 23.97 24.25 25.86

As shown in Table 3 above, Comparative Formulations 1 to 3, which contain an extract from Namhae Seaweed, Comparative Forms 4 to 6, which contains a bumela extract, and Comparative Forms 7 to 9, which contain no extract, The moisturizing effect of Formulation Examples 1 to 9 containing the mixed extract of the extract from May and the extract of Buamera was excellent.

Experimental Example  2: The beginning of the month  Moisturizing Effect of Extract and Buamera Extract 2 - Skin barrier  Improvement effect

25 ℃, relative humidity of 45%, 160 healthy women in the room without air flow. Skin barrier damage was induced using a 1% aqueous solution of sodium lauryl sulfate (SLS) and the cosmetic compositions of Formulations 1 to 9 and Comparative Formulations 1 to 6 and 9 were applied to the upper arm of the arm The transdermal water loss was measured using a TEWAMETER TM210 (C + K eletronic GmHH, Germany). That is, TEWL (Transepidermal Water Loss) value was measured by changing the TEWL value with time, and skin barrier recovery rate (%) was confirmed. The experimental results are shown in Table 4 below.

division Skin barrier recovery rate (%) 2D 4D 6D 8D Formulation Example 1 52.41 67.08 86.41 98.57 Formulation Example 2 52.94 67.76 87.28 99.57 Formulation Example 3 51.89 65.08 83.94 96.36 Formulation Example 4 52.42 65.74 85.02 97.34 Formulation Example 5 51.99 65.67 83.69 97.87 Formulation Example 6 52.1 66.7 85.9 98 Formulation Example 7 51.58 66.03 85.04 97.02 Formulation Example 8 52.52 66.34 84.54 98.86 Formulation Example 9 51.7 65.3 83.2 97.3 Comparative Formulation Example 1 42.08 55.05 72.48 84.84 Comparative Formulation Example 2 42.15 55.64 73.36 86.32 Comparative Formulation Example 3 41.66 55 72.52 85.35 Comparative Formulation Example 4 42.6 55.72 73.3 85.8 Comparative Formulation Example 5 42.37 55.03 70.86 85.39 Comparative Formulation Example 6 42.05 56.45 75.18 86.04 Comparative Formulation Example 9 37.8 51.5 61.5 69.4

As can be seen from the above Table 4, Formulations 1 to 9 containing the mixed extract of Mulberry leaf extract and Buamera extract of the present invention have improved transdermal water loss as compared with the other comparative formulation examples.

Experimental Example 3: Safety test of the extracts of Namolwol and Mulberry extract

In order to confirm skin irritation on the cosmetic compositions of Formulation Examples 1 to 9 and Comparative Formulation Examples 1 to 6 and 9, a human skin patch test was conducted. Twenty women in their 20s and 30s who have never been hypersensitive to skin irritation due to a history of skin irritation or skin allergy have been tested.

First, the test area was wiped with 70% ethanol and then dried. The prepared test material was dripped into a Finn chamber (100X10, EPITEST), and the test piece was hermetically sealed on the back of the test subject. After 24 hours of exposure, the patches were removed, and 1 hour and 24 hours after the removal of the patch, erythema and edema were observed using a magnifying glass (8MC-150, DAZOR).

The skin reaction was judged according to the rules of the International Contact Dermatitis Research Group (ICDRG) (Table 5). The average skin reactivity was calculated by the following formula (1) and the results are shown in Table 6.

sign score Evaluation standard - 0 No reaction ± 0.5 Faint or mild erythema + One The border is clear but weak erythema, edema and palsy ++ 2 Clear erythema, papules and small follicles +++ 3 Severe erythema and alveoli, scab formation

division 24 hours 48 hours Average skin response ± + ++ ± + ++ (n = 20) Formulation Example 1 One - - - - - 0.18 Formulation Example 2 One - - - - - 0.18 Formulation Example 3 One - - - - - 0.18 Formulation Example 4 - - - - - - 0 Formulation Example 5 One - - - - - 0.18 Formulation Example 6 One - - - - - 0.18 Formulation Example 7 - - - - - - 0 Formulation Example 8 One - - - - - 0.18 Formulation Example 9 - - - - - - 0 Comparative Formulation Example 1 One - - - - - 0.18 Comparative Formulation Example 2 - - - - - - 0 Comparative Formulation Example 3 One - - - - - 0.18 Comparative Formulation Example 4 One - - - - - 0.18 Comparative Formulation Example 5 - - - - - - 0 Comparative Formulation Example 6 One - - - - - 0.18 Comparative Formulation Example 9 2 - - - - - 0.28

As shown in Table 6, all of the cosmetic compositions tested did not induce skin irritation. Thus, it can be seen that there is no abnormality in the safety of cosmetic formulations to which an extract of Oriental japonica and an extract of Bomamera are added.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (5)

A cosmetic composition for moisturizing, comprising a mixture of extracts of Namhae Seed extract and Buamera extract. The cosmetic composition according to claim 1, wherein the cosmetic composition has a skin barrier function recovery effect. [Claim 2] The cosmetic composition according to claim 1, wherein the mixed extract of Namyeolcho extract and Buamera extract is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. [Claim 2] The method according to claim 1, wherein the mixed extract of Namyeolcho extract and Buamera extract comprises (a) water, an anhydrous or a lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform (B) supercritical extraction, (c) ultrasonic extraction, and (d) fermentation extraction using a microorganism. ≪ / RTI > wherein the composition is extracted by an extraction method selected from the group consisting of: The cosmetic composition according to claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray ≪ / RTI > is a formulation selected from the group consisting of: