KR101879644B1 - Cosmetic composition for skin moisturizing containing furcellaria lumbricalis extract and perna canaliculus extract - Google Patents

Abstract

The present invention was blue Celia extract (Furcellaria as lumbricalis) and Abstract of the lipped mussel extract (Perna Canaliculus) a cosmetic composition containing, as an active ingredient, and more particularly, the skin moisturizing effect and skin barrier strengthening effect related to superior skin protection cosmetic composition.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a cosmetic composition for moisturizing cosmetics containing a green tea extract, a green tea extract, a green tea extract, a green tea extract, a green tea extract, a green tea extract,

The present invention relates to furcellaria The present invention relates to a cosmetic composition containing an extract of Lumbricalis and a green lipped mussel extract ( Perna Canaliculus ) as an active ingredient. More particularly, the present invention relates to a cosmetic composition containing an extract of Purcellia extract and green lipped mussel extract as an active ingredient, The present invention relates to a cosmetic composition for skin protection having excellent barrier strengthening effect.

The skin protects the body from various external stimuli and has a barrier function to prevent the inner moisture. In the epidermis of the skin, it develops as a thin stratum corneum containing water through differentiation process. Structurally, the cells in the stratum corneum act like bricks, and the intercellular lipids of the keratinocytes act as mortar to form the skin barrier.

However, the human body tends to increase in dry skin due to aging, and the need for a skin moisturizing agent is increasing due to air pollution and indoor heating. For this purpose, saccharides and polyhydric alcohols are used as a moisturizing agent as a general moisturizing agent in cosmetics However, these moisturizers have a disadvantage in that the skin becomes sticky, and there is a problem that the moisturizing effect and the continuous moisturizing effect are not given to the skin. In addition, in the summer when the humidity is very high, moisture is drawn from the outside and the skin is sticky. In winter, the humidity of the outside air is low, and rather, the moisture is drawn from the skin and the skin is dried.

Water acts as a carrier of the physiologically active substance required for the human body and allows the skin to maintain a smooth and moist condition. However, its functions can easily be lost due to environmental factors such as excessive washing, bathing, environmental factors such as dry atmosphere, pollutants, etc., and in recent years, due to the increase of various harmful factors, The number of people suffering from various disabilities is increasing.

Various cosmetic compositions have been tried to improve the dryness of the skin by using various moisturizing agents such as sugar, polyol and the like. For example, natural moisturizing factors in the stratum corneum, which suppress moisture evaporating from the skin or easily combine with water molecules on the skin surface to maintain moisture in the stratum corneum, by strengthening the skin barrier function using ceramide, cholesterol, (Hyaluronic Acid), sugars and polyols that have strong ability to bind to natural moisturizing factors (NMF), sodium pyrrolidonecarboxylate, lactate and water, .

Furthermore, in recent years, many cosmetics using natural products have been developed to reduce skin irritation caused by external chemicals and the like. Natural products are not only less harmful to the skin, but they are also increasingly being developed as cosmetics raw materials as consumers' demand for cosmetics using natural ingredients increases.

Accordingly, the present inventors have studied the applicability of various natural materials not yet known to cosmetics, and as a result, found that Furcellaria lumbricalis ) and green lipped mussel ( Perna Canaliculus ) was selected and the mixed extracts were prepared. The results showed that the moisturizing effect and the skin barrier strengthening effect were measured. As a result, it was found that the efficacy of the cosmetic product can be expected .

It is an object of the present invention to provide a furcellaria lumbricalis ) and green lipped mussel ( Perna Canaliculus ) mixed extract to exhibit a moisturizing effect and a skin barrier strengthening effect.

It is also an object of the present invention to provide Furcellaria lumbricalis , and Perna Canaliculus extract, and to provide a cosmetic composition exhibiting an efficacy effect by preparing a supercritical, ultrasonic, and fermented extract and purifying the extract.

The present invention relates to a moisturizing cosmetic composition containing a mixed extract of Furcellia extract and Green Lipped Mussel extract.

Preferably, the cosmetic composition has a skin barrier function recovery effect.

Preferably, the mixed extract of Furchelia extract and Green Lipped Mussel Extract is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the combined extracts of the Furchelia extract and the Green Lipped Mussel Extract are (a) water, an anhydrous or a lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, (B) a supercritical extraction method, (c) an ultrasonic extraction method, and (d) a fermentation extraction method using a microorganism. The method according to claim 1, wherein the solvent extraction is performed using an extraction solvent selected from the group consisting of acetone, methyl ethyl ketone, butyl acetate, diethyl ether, dichloromethane, And extracted by the extraction method selected from the group consisting of

Preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray ≪ / RTI >

In accordance with the present invention, Furcellaria lumbricalis extract and green lipped mussel ( Perna Canaliculus ) extract have excellent moisturizing effect (Experimental Example 1).

Also, Furcellaria lumbricalis extract and green lipped mussel ( Perna Canaliculus ) extract have excellent skin barrier strengthening effect (Experimental Example 2).

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In addition, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The term "about" is used herein to refer to a reference quantity, a level, a value, a number, a frequency, a percent, a dimension, a size, a quantity, a weight, or a length of 30, 25, 20, 25, 10, 9, 8, 7, Level, value, number, frequency, percent, dimension, size, quantity, weight or length of a variable, such as 4, 3, 2 or 1%.

Throughout this specification, the words "comprises" and "comprising ", unless the context requires otherwise, include the steps or components, or groups of steps or elements, Steps, or groups of elements are not excluded.

Greener Celia (Furcellaria lumbricalis) used as an active ingredient of the cosmetic composition of the present invention is a seaweed belonging to the plant classification red algae greener selriahgwa (Furcellariaceae), and distributed in the Scandinavian coast and all over the world, the size is 20 ~ 30 cm, The width is 2 to 3 mm. It is deep red, very soft and mucilaginous. It has a lot of side branches facing each other on both sides.

Purcellia belongs to the seaweed, and seaweed is a term referring to all of the algae living in the sea. It is classified into green algae, brown algae and red algae according to the depth and color of the sea which is generally grown. Seaweeds are rich in alginic acid, fucoidan, laminin, fucosterol, taurine, mannitol, calcium, iodine, and vitamins that have excellent physiological activity. Therefore, they can be used for various diseases such as hypertension, arteriosclerosis, diabetes, obesity, malignant tumors, , And are known to have antiviral efficacy.

In the present invention, the Furchelia extract means obtained by extracting from various organs or parts of Furchelia, preferably an extract obtained from the outposts or the sites of the central branch and lateral bodies, and most preferably, Means the extract obtained from the site of.

Also, the green lipped mussel ( Perna Canaliculus ) used as an active ingredient in the cosmetic composition of the present invention is a kind of mussel that lives on the coast of New Zealand and is also called green-lipped mussel.

The green lipped mussel contains a large amount of various minerals besides omega 3 which is an unsaturated fatty acid, and is known to be effective for anti-inflammation, arthritis, rheumatism and spondylitis.

In the present invention, the green lipped mussel extract is obtained by extracting from various organs or parts of green lipped mussel.

In addition, the Purselia extract and the green lipped mussel extract of the present invention can be produced according to a conventional method known in the art. For example, extraction is performed using (a) a solvent extraction method, (b) a supercritical extraction method using reduced pressure by carbon dioxide, or (c) an ultrasonic extraction method.

In the present invention, the Furcellia extract and the green lipped mussel extract may be dissolved in various extraction solvents, for example, water, anhydrous or lower alcohols having 1-4 carbon atoms (for example, methanol, ethanol, propanol and butanol) , Butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, nucleic acids, and mixtures thereof, preferably using an extraction solvent Is obtained by using ethanol, 70% (v / v) ethanol or water, and most preferably by using 70% (v / v) ethanol.

It will be apparent to those skilled in the art that the extracts of Purselia and Green Lipped Mussel of the present invention can be obtained not only by the above-mentioned extraction solvents but also by other extraction solvents.

In addition, the Furchelia extract and the green-lipped mussel extract of the present invention include not only the extract obtained by the above-mentioned extraction solvent, but also the ordinary purified and fermented extract. For example, decompression by carbon dioxide, extraction by supercritical extraction with high temperature, extraction by extraction using ultrasound, separation by ultrafiltration membrane with constant molecular weight cutoff value, various chromatography (size, charge, hydrophobic or affinity And the active fraction obtained through various purification and extraction methods such as an extract made by a fermentation product using natural or various microorganisms are also included in the extract of the present invention.

Further, the present invention provides a cosmetic composition characterized in that the above extract is obtained by liquid-freezing obtained by cold-pressing and heating filtration at room temperature, and further by concentrating or lyophilizing the solvent under reduced pressure.

The supercritical fluid extraction refers to supercritical fluid extraction. Generally, the supercritical fluid is extracted from the liquid and the liquid when the gas reaches the critical point at high temperature and high pressure. (J. Chromatogr. A. 1998; 479: 200-205). In addition, it has been reported that the supercritical fluid has a polarity similar to that of a nonpolar solvent.

Carbon dioxide is a supercritical fluid with both liquid and gaseous nature, with the operation of supercritical fluid equipment reaching its critical pressure and temperature, resulting in increased solubility in fat-soluble solutes. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the lipophilic substance contained in the sample is extracted into supercritical carbon dioxide.

When the supernatant carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction vessel after extracting the lipid-soluble substance, components that were not extracted by pure supercritical carbon dioxide can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract an active ingredient by using a mixed fluid in which a cosolvent is further mixed with supercritical carbon dioxide or carbon dioxide.

These co-solvents may be used alone or in admixture of two or more selected from the group consisting of chloroform, olive oil, ethanol, methanol, water, ethyl acetate, nucleic acid and diethyl ether. Preferably, one or a mixture of two or more selected from the group consisting of olive oil, ethanol, methanol, and water can be used.

Most of the extracted samples contain carbon dioxide. Since the carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the cosolvent can be removed by a reduced pressure evaporator.

In addition, the ultrasonic extraction method is an extraction method using energy generated by ultrasonic vibration. Ultrasonic waves can destroy an insoluble solvent contained in a sample in a water-soluble solvent. Due to the high local temperature generated at this time, Since the kinetic energy of the particles is increased, sufficient energy required for the reaction is obtained. By inducing the high pressure by the shock effect of the ultrasonic energy, the mixing efficiency of the substance contained in the sample and the solvent is increased, thereby increasing the extraction efficiency.

As the extraction solvent usable in the ultrasonic extraction method, one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, nucleic acid and diethyl ether can be used. The extracted sample is recovered by vacuum filtration, the filtrate is recovered, and the extract is removed by a vacuum evaporator and lyophilized to obtain an extract.

The Furchelia extract and the Green Lipped Mussel extract according to the present invention also include fermented extracts, which can be prepared as follows. Furcellia and green lipped mussel are finely crushed to about 100 to 500 mesh and then 1 to 50 g / L of a conventional microorganism culture solution is added and microorganisms such as yeast strains or lactic acid bacteria are added in an amount of 10,000 to 100,000 cfu / L. The culture temperature is 30 to 37 ° C. The pH is 5 to 7 and aerobic or anaerobic conditions are maintained for about 5 to 10 days. Which can be obtained through aging and filtration.

According to the present invention, the optimum mixing ratio of the Furcellia extract and the greenish-lipped mussel extract is 1: 10 to 1: 10, more preferably 1: 1, . Meanwhile, the mixed extract of the present invention may be extracted after mixing the green and the green lipped mussels, or may be mixed after obtaining the respective extracts.

The mixed extract of Furchelia extract and Green Lipped Mussel Extract is contained in an amount of 0.001 to 30.0% by weight, preferably 0.01 to 20% by weight, more preferably 0.1 to 10% by weight based on the total weight of the cosmetic composition %. When the content of the extract is less than 0.001% by weight, the skin moisturizing effect is not exhibited. When the content is more than 30.0% by weight, the skin moisturizing effect is insignificant to the increase of the content, It is not even.

In order to use the Furchelia extract and the Green Lipped Mussel extract prepared by a conventional method in cosmetic formulations, it is possible to include a cosmetic composition that shows efficacy after removing unnecessary components through purification. The purification can be carried out in any conventional purification process, including liquid-liquid extraction using solubility differences, precipitation and filtration, chromatography using polarity and molecular weight, and the like. For example, the extracts of Purcellia and green mussel extracts were dissolved in aqueous ethanol solution (50%, 70% solution, etc.) or other aqueous solution, and then the column filled with HP-20 and sephadex columns was used to determine the polarity and molecular weight difference , The water-soluble substance and the oil-soluble substance can be separated, and the oil-soluble substance can be concentrated and freeze-dried by TLC to prepare a concentrate.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, and may contain conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, Supplements, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powdered foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the cleansing containing the surfactant in the formulations of the present invention, it is preferable to use, as the carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, washed or rinsed with water. The surfactant-containing cleansing formulation may be a cleansing foam, a cleansing water, a cleansing towel and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Manufacturing example  One: Purcellia  Solvent extract

300 g of the shrubs and shrubs of the shrubs of the purple celia were refluxed three times for 5 hours with 70% (v / v) aqueous ethanol solution, cooled with ice, and filtered through Whatman # 4 filter paper. The filtered extract was washed with an ultrafiltration membrane and concentrated under reduced pressure and freeze-dried at 50 ° C or less to obtain a Purselia extract powder. Then, the dried Purselia extract powder was dissolved in 50% 1,3-butylene glycol and used.

Manufacturing example  2: Purcellia Supercritical  Fluid extract

The center and branches of the purplish purple celia and 300 g of the lateral upper body were extracted with olive oil as a co-solvent under supercritical extraction under a pressure of about 300 atmospheres (atm) at a temperature of about 60 캜 After removing the salt by using an ultrafiltration membrane, it was concentrated under reduced pressure and lyophilized to obtain a powder of Purselia extract. This was dissolved in 50% 1,3-butylene glycol and used.

Manufacturing example  3: Purcellia  Ultrasonic Extract Manufacturing

Extracted 300g of the central branch of the shrub purple celia and the site of the upper body were extracted using ultrasound (Extracted at 30 ℃ for about 2 hours at 25 KHz intensity using SuperNonic Ultrasonic apparatus) And then concentrated under reduced pressure and lyophilized to obtain a powder of Furcellia extract. The dried Purselia extract powder was dissolved in 50% 1,3-butylene glycol and used.

Manufacturing example  4: Green mouth  Mussel solvent extract

300 g of the various organs of shredded green lipped mussel were refluxed for 3 hours with 70% (v / v) ethanol aqueous solution for 3 hours, cooled, and filtered through Whatman # 4 filter paper. After removing the salt by using an ultrafiltration membrane, the filtrate was concentrated under reduced pressure at 50 ° C or less and lyophilized to obtain green lipped mussel extract powder. Then, the dried green lipped mussel extract powder was dissolved in 50% 1,3-butylene glycol and used.

Manufacturing example  5: Green mouth  mussel Supercritical  Fluid extract

300 g of the various organs of the shredded green lipped mussel are extracted using conventional supercritical extraction (using olive oil as co-solvent in a supercritical state at a pressure of about 300 atmospheres (atm) at a temperature of about 60 DEG C) And extracted with ultrafiltration membrane. The extract was concentrated under reduced pressure and lyophilized to obtain green lipped mussel extract powder. This was dissolved in 50% 1,3-butylene glycol and used.

Manufacturing example  6: Green mouth  Manufacture of mussel ultrasound extract

Extracted 300 g of various organs or parts of shaded green lipped mussel were extracted using ultrasonic (SuperNonic Ultrasonic Device at 25 KHz intensity at 30 ° C for about 2 hours), and the salts were removed using an ultrafiltration membrane After extraction, the extract was lyophilized and lyophilized to obtain green lipped mussel extract powder. Dried green lipped mussel extract powder was dissolved in 50% 1,3-butylene glycol and used.

Formulation Example  Preparation of 1 to 9: Purcellia  The extract and Green mouth  Mussel Cosmetics  Composition

(1) to (3) and the green-lipped mussel extracts of Production Examples 4 to 6 were mixed at a weight ratio of 1: 1, respectively, and the cosmetic compositions (Formulation Examples 1 to 3) containing a mixed extract of Furchelia extract and Green- 9) were prepared and are shown in Table 1 below.

ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 Formulation Example 7 Formulation Example 8 Formulation Example 9 Production Example 1 2.5 2.5 2.5 - - - - - - Production Example 2 - - - 2.5 2.5 2.5 - - - Production Example 3 - - - - - - 2.5 2.5 2.5 Production Example 4 2.5 - - 2.5 - - 2.5 - - Production Example 5 - 2.5 - - 2.5 - - 2.5 - Production Example 6 - - 2.5 - - 2.5 - - 2.5 glycerin - - - - - - - - - 1,3-butylene glycol - - - - - - - - - Sorbitol - - - - - - - - - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Cetostearyl alcohol 2 2 2 2 2 2 2 2 2 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystalline 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Squalane 5 5 5 5 5 5 5 5 5 Liquid paraffin 2 2 2 2 2 2 2 2 2 Trioctanoin 3 3 3 3 3 3 3 3 3 Polysorbate One One One One One One One One One Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl acetate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Cyclomethicone 4 4 4 4 4 4 4 4 4 BHT 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Comparative Formulation Example  Preparation of 1 to 9: Purcellia  The extract and Green mouth  Mussel extract Cosmetics  Compositions and compositions containing them Cosmetics  Composition

Cosmetic compositions (Comparative Formulation Examples 1 to 3) containing the Furchelia extracts of Production Examples 1 to 3, Cosmetic preparations (Comparative Formulation Examples 4 to 6) containing the green-lipped mussel extract of Production Examples 4 to 6, Furchelia extract or Green Cosmetic compositions (Comparative Formulation Examples 7 to 8) containing glycerin and 1,3-butylene glycol as moisturizers (Comparative Formulation Examples 7 to 8) and cosmetic compositions (Comparative Formulation Example 9) containing no extracts or moisturizing agents were prepared as shown in Table 2 below .

ingredient Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Comparative Formulation Example 4 Comparative Formulation Example 5 Comparative Formulation Example 6 Comparative Formulation Example 7 Comparative Formulation Example 8 Comparative Formulation Example 9 Production Example 1 5 - - - - - - - - Production Example 2 - 5 - - - - - - - Production Example 3 - - 5 - - - - - - Production Example 4 - - - 5 - - - - - Production Example 5 - - - - 5 - - - - Production Example 6 - - - - - 5 - - - glycerin - - - - - - 5 5 - 1,3-butylene glycol - - - - - - 5 - - Sorbitol - - - - - - - 5 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Cetostearyl alcohol 2 2 2 2 2 2 2 2 2 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystalline 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Squalane 5 5 5 5 5 5 5 5 5 Liquid paraffin 2 2 2 2 2 2 2 2 2 Trioctanoin 3 3 3 3 3 3 3 3 3 Polysorbate One One One One One One One One One Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl acetate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Cyclomethicone 4 4 4 4 4 4 4 4 4 BHT 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Experimental Example  One: Purcellia  The extract and Green mouth  Moisturizing effect of mussel extract

The clinical moisturizing effect and the sustaining power for Formulation Examples 1 to 9 and Comparative Formulation Examples 1 to 9 were measured as follows. A total of 180 healthy male and female adults who felt skin dryness were randomly divided into groups of 10, and each of the formulations and the comparative formulations for each group were administered twice daily for four weeks, one for each side, Respectively. Moisturizing effect was evaluated by quantifying the increase rate (%) of skin conductivity according to the skin moisture content using the Corneometer CM 820 (Corage + Khazaka, Germany) for 2 weeks from the start of the test and after the completion of the test. The experimental results are shown in Table 3 below.

Increase rate of skin electrical conductivity (%) division Before use After 2 weeks of use After 4 weeks of use Formulation Example 1 25.23 44.22 52.54 Formulation Example 2 24.48 43.57 52.8 Formulation Example 3 23.63 44.88 52.88 Formulation Example 4 25.49 44.64 52.72 Formulation Example 5 24.72 43.99 51.73 Formulation Example 6 23.87 45.3 53.39 Formulation Example 7 25.02 43.93 52.96 Formulation Example 8 25.87 44.36 53.64 Formulation Example 9 23.85 43.25 51.74 Comparative Formulation Example 1 25.36 38.38 42.78 Comparative Formulation Example 2 24.61 36.8 41.72 Comparative Formulation Example 3 23.75 37 41.66 Comparative Formulation Example 4 25.62 38.78 42.77 Comparative Formulation Example 5 24.85 37.2 41.89 Comparative Formulation Example 6 23.99 37.41 42.15 Comparative Formulation Example 7 25.15 34.28 38.99 Comparative Formulation Example 8 26 35.64 37.74 Comparative Formulation Example 9 23.97 23.25 24.86

As shown in Table 3 above, Comparative Formulations 1 to 3 containing the Furchelia extract, Comparative Formulations 4 to 6 containing the green lipped mussel extract, and Comparative Formulations 7 to 9 containing no extract, It can be seen that Formulation Examples 1 to 9 containing a mixed extract of Furcellia extract and Green Lipped Mussel extract have excellent moisturizing effect.

Experimental Example  2: Purcellia  The extract and Green mouth  Mussel Skin barrier  Improvement effect

25 ℃, relative humidity of 45%, 160 healthy women in the room without air flow. Skin barrier damage was induced using a 1% aqueous solution of sodium lauryl sulfate (SLS) and the cosmetic compositions of Formulations 1 to 9 and Comparative Formulations 1 to 6 and 9 were applied to the upper arm of the arm The transdermal water loss was measured using a TEWAMETER TM210 (C + K eletronic GmHH, Germany). That is, TEWL (Transepidermal Water Loss) value was measured by changing the TEWL value with time, and skin barrier recovery rate (%) was confirmed. The experimental results are shown in Table 4 below.

Skin barrier improvement effect of green tea extract and green lipped mussel extract division Skin barrier recovery rate (%) 2D 4D 6D 8D Formulation Example 1 52.61 67.28 86.61 98.77 Formulation Example 2 53.14 67.96 87.48 99.77 Formulation Example 3 52.09 65.28 84.14 96.56 Formulation Example 4 52.62 65.94 85.22 97.54 Formulation Example 5 52.19 65.87 83.89 98.07 Formulation Example 6 52.3 66.9 86.1 98.2 Formulation Example 7 51.78 66.23 85.24 97.22 Formulation Example 8 52.72 66.54 84.74 99.06 Formulation Example 9 51.9 65.5 83.4 97.5 Comparative Formulation Example 1 42.28 55.25 72.68 85.04 Comparative Formulation Example 2 42.35 55.84 73.56 86.52 Comparative Formulation Example 3 41.86 55.2 72.72 85.55 Comparative Formulation Example 4 42.8 55.92 73.5 86 Comparative Formulation Example 5 42.57 55.23 71.06 85.59 Comparative Formulation Example 6 42.25 56.65 75.38 86.24 Comparative Formulation Example 9 38 51.7 61.7 69.6

As can be seen from Table 4, Formulations 1 to 9 containing the mixed extract of Furchelia extract and Green Lipped Mussel extract of the present invention have improved transdermal water loss as compared to the other comparative formulations.

Experimental Example 3: Safety test of the extract of Furchelia and green-lipped mussel

In order to confirm skin irritation on the cosmetic compositions of Formulation Examples 1 to 9 and Comparative Formulation Examples 1 to 6 and 9, a human skin patch test was conducted. Twenty women in their 20s and 30s who have never been hypersensitive to skin irritation due to a history of skin irritation or skin allergy have been tested.

First, the test area was wiped with 70% ethanol and then dried. The prepared test material was dripped into a Finn chamber (100X10, EPITEST), and the test piece was hermetically sealed on the back of the test subject. After 24 hours of exposure, the patches were removed, and 1 hour and 24 hours after the removal of the patch, erythema and edema were observed using a magnifying glass (8MC-150, DAZOR).

The skin reaction was judged according to the rules of the International Contact Dermatitis Research Group (ICDRG) (Table 5). The average skin reactivity was calculated by the following formula (1) and the results are shown in Table 6.

sign score Evaluation standard - 0 No reaction ± 0.5 Faint or mild erythema + One The border is clear but weak erythema, edema and palsy ++ 2 Clear erythema, papules and small follicles +++ 3 Severe erythema and alveoli, scab formation

division 24 hours 48 hours Average skin response ± + ++ ± + ++ (n = 20) Formulation Example 1 One - - - - - 0.18 Formulation Example 2 One - - - - - 0.18 Formulation Example 3 One - - - - - 0.18 Formulation Example 4 - - - - - - 0 Formulation Example 5 One - - - - - 0.18 Formulation Example 6 One - - - - - 0.18 Formulation Example 7 - - - - - - 0 Formulation Example 8 One - - - - - 0.18 Formulation Example 9 - - - - - - 0 Comparative Formulation Example 1 One - - - - - 0.18 Comparative Formulation Example 2 - - - - - - 0 Comparative Formulation Example 3 One - - - - - 0.18 Comparative Formulation Example 4 One - - - - - 0.18 Comparative Formulation Example 5 - - - - - - 0 Comparative Formulation Example 6 One - - - - - 0.18 Comparative Formulation Example 9 2 - - - - - 0.28

As shown in Table 6, all of the cosmetic compositions tested did not induce skin irritation. Accordingly, it can be seen that the safety of the cosmetic formulation containing the Purselia extract and the green lipped mussel extract is not impaired.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (5)

A cosmetic composition for moisturizing, comprising a mixture of extracts of Purselia extract and green lipped mussel extract,
The Furchelia extract and the Green Lipped Mussel extract are mixed at a weight ratio of 1 to 10: 1 to 10,
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray ≪ / RTI > The cosmetic composition according to claim 1, wherein the cosmetic composition has a skin barrier function recovery effect. [Claim 3] The cosmetic composition according to claim 1, wherein the mixed extract of Furchelia extract and Green Lipped Mussel extract is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. [Claim 2] The method according to claim 1, wherein the mixed extract of the Furchelia extract and the green lipped mussel extract comprises (a) water, an anhydrous or a lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, (B) a supercritical extraction method, (c) an ultrasonic extraction method, and (d) a fermentation using a microorganism. The method according to claim 1, wherein the solvent is selected from the group consisting of chloroform, butyl acetate, diethyl ether, dichloromethane, Wherein the extract is extracted by an extraction method selected from the group consisting of extraction methods. delete