KR101671269B1 - Cosmetic composition with the extract of Tillandsia usneoides for skin moisturizing, keratinocyte differentiation enhancement and skin barrier function recovery - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an extract of Tailor chinensis, and the present invention relates to a cosmetic composition containing the extract of Tailor chinensis extract, which promotes the differentiation of dermal keratinocytes in the extract of Tailor chinensis, restores skin barrier function, It can be effectively used as a cosmetic for moisturizing skin, promoting differentiation of dermal keratinocyte, and restoring skin barrier function.

Description

Technical Field [0001] The present invention relates to a cosmetic composition for skin moisturizing, skin keratinocyte differentiation promotion, skin barrier function recovery, and cosmetic composition containing barley tilandia chrysanthemum extract and keratinocyte differentiation enhancement and skin barrier function recovery,

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition for skin moisturizing, skin keratinocyte differentiation promotion, skin barrier function recovery containing a hair tilylandia extract.

The skin is largely divided into three layers: epidermis, dermis, and subcutaneous tissue in order from the outside. It protects the internal organs of the body from changes in temperature and humidity, ultraviolet rays, and other physical and chemical external stimuli. Among them, the epidermis plays an important role in preventing water evaporation inside the human body.

In healthy human keratinocytes, a high concentration of Natural Moisturing Factor (NMF) is present to help maintain moisture in the skin. For example, substances such as amino acids are water-soluble and thus effectively combine with moisture to dry the skin (J. Invest. Dermatol. 54, 24-31, 1970).

On the other hand, industrialization due to the development of science has caused a drastic change in the global environment, which has had a serious impact on human health. As a result, various chronic and intractable diseases are rapidly increasing, and the incidence of environmental diseases such as atopic dermatitis, allergic rhinitis and asthma is increasing worldwide.

According to data released by the National Health Insurance Corporation's Health Insurance Research Institute during 2002-2007, the number of patients suffering from environmental diseases such as allergic rhinitis, atopic dermatitis and asthma was estimated to reach 552 million The number of health-related diseases increased from 29.3% in 2007 to 7.14 million in 2007 (3.25 million men and 3.79 million women), which is a threat to national health and health insurance finances, In fact.

In order to prevent the aforementioned environmental diseases, skin moisturization is indispensable. In the past, humectants having moisture-absorbing properties and Occlusive Moisturizers preventing evaporation of water have been used, and moisture content in the stratum corneum Has been performed. Examples of humectant include glycerin, propylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, sodium 2-pyrrolidone-5-carboxylate and the like. There is a disadvantage that it feels bad or exhausted. (5), 731-740, 1994), there is a difficulty in maintaining the stability of the emulsified formulations, and there is a problem in that a transparent gel phase There is a disadvantage that it is not suitable for manufacturing a product.

However, recently it has been argued that the differentiation of the stratum corneum is important in preventing the drying of skin moisture. It has been found that the differentiation process developed from the keratinocytes of the basal layer to the keratinocytes of the outermost keratinocyte can function as a barrier to skin moisture retention. In other words, during the process of keratinization, the cells produce natural moisturizing factors and intercellular lipids, and as a result, the stratum corneum becomes rigid and supple, allowing it to function as a barrier. Physiological tendency of the skin to become dry as the age of the human is increased can be interpreted as a longer time for the removal of the stratum corneum, a lowering of the lipid synthesis ability of the epidermal cells, and a decrease in the amount of the moisturizing factor and lipid in the stratum corneum . Therefore, it is reasonable to propose a new approach to promote the differentiation of keratinocytes to induce strengthening of skin barrier, thereby enhancing skin moisture retaining function and protection from external environment.

On the other hand, Tillandsia usneoides is a parasitic plant of the pineapple tree, and there are about 500 species of Tilandia spp. The plants are silver-gray and often grow together like a whisker. It is also called a public plant (air plant) because it does not need soil for its survival, it stays in the same place as trees and survives in the air. Like the normal plants, roots do not play a big role, they are used only for hanging. The hair like hair is scaly and absorbs water vapor and organic matter by using fine fleece. Unlike ordinary plants, it does not emit carbon dioxide during the day, and it has excellent air purifying function that produces oxygen even at night, and it is in the spotlight as a pet plant.

Beetle tilandiaea extract contains a large amount of sugar components, amino acids, minerals, vitamins, and betaine. However, at present, only a study on the role of purifying air as a public plant has been made, and research as a cosmetic composition has been limited.

Korean Patent Laid-Open Publication No. 10-2008-0088735 (published on Oct. 10, 2008) describes a cosmetic composition excellent in moisturizing effect by containing a moisturizing complex composed of "yogurt powder; fruit extract or cereal extract; have.

The present invention provides a composition capable of providing an excellent moisturizing effect to the skin and improving the function of the skin barrier.

The present invention provides, as a first aspect, a cosmetic composition for moisturizing skin characterized by containing anhydrous tilland extract as an active ingredient.

The present invention also provides a cosmetic composition for accelerating the differentiation of keratinocyte cells, which comprises, as a second aspect, an extract of Hydrangea chrysanthemum as an active ingredient.

The present invention also provides a cosmetic composition for restoring skin barrier function, which comprises, as a third aspect, an extract of Hydrangea tigris extract as an active ingredient.

Meanwhile, in any one of the first, second, and third aspects of the present invention, the extract of Tyrandella chinensis is obtained by extracting a supercritical solvent with Tyrrhansia; Extracts obtained by adding ultrasonic waves to Tailelandia; Extracts obtained by adding ultrahigh pressure to beard tillandiae; And extracts obtained by extracting an extracting solvent selected from the group consisting of water, anhydrous or hydrated lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform, butyl acetate and 1,3-butylene glycol on a tiller tiller; Or the like.

The cosmetic composition according to any one of the first, second and third aspects of the present invention may be an aqueous solution, suspension, emulsion, paste, gel, cream, lotion, powder, A soap, a surfactant-containing cleansing, an oil, a powder foundation, an emulsion foundation, a wax foundation, and a spray.

Hereinafter, the contents of the present invention will be described in more detail.

In the present invention, it is intended to provide an effective ingredient of a cosmetic composition after confirming its usefulness for skin moisturizing, promoting differentiation of dermal keratinocyte, and restoring skin barrier function of Tailor larvae extract. The hydrolyzate extract of the present invention can be obtained in the form of an extract from barley tillandiae using a conventional solvent according to a conventional method known in the art, that is, under the conditions of ordinary temperature and pressure.

The hydrolyzate extract of the present invention includes, for example, purified water, methanol, ethanol, glycerin, ethyl acetate, butyleneglycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butyleneglycol and hexane , Preferably ethanol, 70% ethanol, or water. The extract may be extracted using one or more extraction solvents selected from ethanol, 70% ethanol or water. However, it is apparent to those skilled in the art that the present invention can be used to obtain an extract having substantially the same effect by using not only the aforementioned extraction solvent but also other extraction solvents.

Meanwhile, the bait tilelandiae extract of the present invention can be extracted by supercritical extraction using supercritical extraction solvent. Supercritical fluid extraction refers to supercritical fluid extraction. The supercritical fluid is extracted from liquid or gas when the gas reaches the critical point at high temperature and high pressure. (J. Chromatogr. A. 1998; 479: 200-205) because it has chemically similar polarity to a non-polar solvent. Since the supercritical fluid solvent can be easily removed from the product, it is possible to prevent environmental pollution, which is a problem in extraction of organic solvent, and it is also an economical and eco-friendly extraction method because its price is low. In addition, since the supercritical extraction method uses carbon dioxide which is harmless to the human body instead of a solvent, it is non-toxic to the skin and has a merit that the freshness of natural plants and herbal medicines can be delivered as they are processed at a low temperature. Finally, the extraction process can be simplified as much as possible and biochemically stable.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract an active ingredient by using, for example, a mixed fluid in which a cosolvent is further mixed with supercritical carbon dioxide or carbon dioxide. These co-solvents may be used alone or in admixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether. Most of the extracted samples contain carbon dioxide. Since the carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the cosolvent can be removed by a reduced pressure evaporator.

On the other hand, the present invention may be produced by using an ultrasonic extraction method for applying ultrasound or an ultrahigh pressure extraction method for applying ultrahigh pressure. Since the detailed processes of the ultrasonic extraction method and the ultra-high pressure extraction method are already well known, detailed description thereof will be omitted.

In addition, the hydrolyzate extract of the present invention may be a liquid obtained by cold-pressing or heating the hydrolyzate extract at room temperature, followed by filtration, or may be obtained by concentration under reduced pressure or lyophilization.

On the other hand, the present invention also relates to the extract of Tailor chrysanthemum extract of the present invention as well as the extract obtained by the ordinary purification process after the extraction. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, The active fraction is also included in the extract of the present invention.

In the present invention, the separation or filtration of the tiller extract of Haematilium can be carried out by using a conventional filtration apparatus such as a Wattmann filter paper, a filtration apparatus with 100 to 400 mesh or the like, a vacuum filtration apparatus, Stirring and rotation by gravity) can be used.

On the other hand, the hairy tilyland extract of the present invention may be contained in an amount of 0.001 to 100.0% by weight, preferably 0.1 to 60.0% by weight, and most preferably 0.1 to 30.0% by weight based on the whole cosmetic composition .

According to the present invention, the whitish tillyland extract of the present invention is excellent in skin erythogenic cell differentiation promoting effect, skin barrier function recovery effect, and moisturizing effect. The cosmetic composition of the present invention contains, as an active ingredient, And include conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavoring agents, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation, and spray, but the present invention is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, Can be used.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane, such as butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component. Examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycols, 1,3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycols or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

When the formulation of the present invention is a soap, a surfactant-containing cleansing agent or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off or removed or rinsed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing foam, Cream, cleansing lotion, cleansing water or cleansing gel.

On the other hand, the cosmetic composition of the present invention may be used alone or in combination, or may be used by being coated with a cosmetic composition other than the present invention. In addition, the cosmetic composition of the present invention can be used according to a conventional method of use, and the number of times of use may be varied depending on the skin condition or taste of the user.

Through the present invention, it has been confirmed that the extract of Tailor ryegrass extract promotes differentiation of dermal keratinocytes, restores skin barrier function, and relieves skin pulling and roughness. Therefore, the hairy tilyland extract of the present invention can be useful for skin moisturizing, skin keratinocyte differentiation promotion, skin barrier function recovery cosmetic.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

[ Manufacturing example  One: Beard Tilandia Supercritical  Preparation of extract]

After shredding, 50.0 g of shredder was placed in the extraction vessel and the flow rate of CO ₂ : co-solvent was 47.5 g / mL: 2.5 g / min, and the total flow rate (total flow rate was flowed at a rate of 50 g / min. Ethyl acetate was used as a co-solvent. Extraction temperature was 60 ℃, extraction time was 60 min and pressure was 300 bar.

[ Manufacturing example  2: Beard Tilandia  Preparation of ultra high pressure extract]

The outpasted beard tilandia was shredded, and after crushing, 50.0 g was heated to 50 ° C under a pressure of 1000 MPa using an ultra-high pressure processor, treated for 24 hours, and then cooled to room temperature. Ethanol was added thereto to reflux the extracted ultrafiltration tillandiae to 70% (v / v) ethanol aqueous solution, refluxed three times for 5 hours, cooled, and filtered through a filter of whatman # 10. The filtrate thus obtained was transferred to a concentration tank using a 0.25 μm filter and concentrated under reduced pressure or lyophilized at about 60 ° C.

[ Manufacturing example  3: Beard Tilandia  Preparation of reflux cooling extract]

After shredding, 50.0 g of shrimp was extracted with 5 times of 70% (v / v) aqueous ethanol solution for 5 hours, refluxed for 3 hours, filtered through Whatman # 2 filter paper . The filtered extract was concentrated under reduced pressure and freeze-dried at 60 ° C or lower.

[ Manufacturing example  4: Beard Tilandia  Preparation of Ultrasonic Extract]

The outpasted beard tilandia was shredded, and 50 g of the shredder was extracted with an ultrasonic wave of 25 KHz at 30 ° C for about 2 hours using a supernatural ultrasonic instrument to obtain an ultrasonic extract. Ultrasonic extracts were filtered through whatman # 10 filter paper. The filtrate thus obtained was transferred to a concentration tank using a 0.25 μm filter and concentrated under reduced pressure or lyophilized at about 60 ° C.

[ Manufacturing example  5: Beard Tilandia  Preparation of extract]

The outpasted beard tilandia was shredded and 50 g after shredding was extracted in a 10-fold volume of a 40% (v / v) 1,3-butyleneglycolic aqueous solution at room temperature for 3 days. Extracted extract was filtered with a filter of whatman # 10. The filtrate thus obtained was filtered with a 0.25 μm filter.

[ Manufacturing example  6 to 10: Beard Tilandia  Preparation of extract]

Except that the extraction solvent shown in the following Table 1 was used instead of 70% (v / v) ethanol, and the extracts of tillandiae extracts of Preparation Examples 6 to 12 were obtained in the same manner as in Preparation Example 3, ≪ / RTI >

extract Extraction solvent Dry weight of final extract
(Unit: g) Production Example 6 n-propanol 0.6 Production Example 7 Isopropanol 0.7 Production Example 8 2-butanol 0.2 Production Example 9 Acetone 0.3 Production Example 10 chloroform 0.1 Production Example 11 ethyl alcohol 0.3 Production Example 12 Butyl acetate 0.2

[ Experimental Example  1: promoting effect of keratinocyte differentiation]

Human keratinocytes were cultured in a culture flask, grown to about 80%, and then treated with the extracts of Tyrandella soja bean (Preparation Examples 1 to 5) at concentrations of 10, 50, 100 and 200 ppm After 4 days of treatment, the protein was dissolved by mixing a urea, a denaturant such as sodium dodecyl sulfate (SDS), and a reducing agent such as? -Mercaptoethanol. After that, the remaining stratum corneum was measured for absorbance at 310 nm to analyze the peptide concentration, and the amount of distilled water as a negative control (0%) was compared with that of each sample. At this time, 10 nM of TGF-β was treated as a positive control.

Concentration (ppm) \ promoting keratinocyte differentiation (%) Production Example 1 Production Example 2 Production Example 3 Production Example 4 Production Example 5 10 39.93 30.05 28.12 28.71 29.12 50 63.77 56.52 49.23 52.89 53.97 100 81.21 76.03 65.33 71.69 72.27 200 153.86 144.29 115.7 134.34 135.98 Negative control (distilled water) 0 0 0 0 0 Positive control (TGF-β) 10 nM 145.25 145.25 145.25 145.25 145.25

As can be seen from the results of Table 1, it was confirmed that the hydrolyzate extract of Shiitake mushroom used in the present invention is excellent in the promotion of keratinocyte differentiation, and it was confirmed that the effect of Preparation Example 1 is the most excellent.

[ Prescription example  1: softening longevity]

As an example of the cosmetic material, a softening longevity containing the tilia tilia extract of Preparation Example 1 was prescribed, and the composition was as shown in Table 3 below.

ingredient Content (unit:% by weight) Beard tillandiae extract 10.0 glycerin 5.0 1,3-Butylene glycol 3.0 PIZZY 1500 1.0 Allantoin 0.1 DL-Panthenol 0.3 Lee D. TEE-2NA 0.02 Benzophenone-9 0.04 Sodium hyaruronate 5.0 ethanol 10.0 Octidodeces-16 0.2 Polysorbate 20 0.2 Preservative, fragrance, pigment a very small amount Distilled water Balance Sum 100

[ Prescription example  2: convergent lotion]

As an example of the cosmetic material, a convergent lotion containing the tiller extract of barley tilland of Preparation Example 1 was used, and the prescription examples were as shown in Table 4 below.

ingredient Content (unit:% by weight) Beard tillandiae extract 30.0 glycerin 2.0 1,3-Butylene glycol 2.0 Allantoin 0.2 DL-Panthenol 0.2 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaruronate 3.0 ethanol 15.0 Polysorbate 20 0.3 Location of Hazel extract 2.0 Citric acid a very small amount Preservative, fragrance, pigment a very small amount Distilled water Balance Sum 100

[ Prescription example  3: Nourishing lotion]

As an example of a cosmetic preparation, a nutritional lotion containing Preparation Example 1 of Tailrillia sieboldii was prescribed, and the prescription examples were as shown in Table 5 below.

ingredient Content (unit:% by weight) Beard tillandiae extract 50.0 Glyceryl stearate SE 1.5 Stearyl alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoin 2.0 Dimethicone 0.8 Tocopheryl acetate 0.5 Carboxyvinyl polymer 0.12 glycerin 5.0 1,3-Butylene glycol 3.0 Sodium hyaruronate 5.0 Triethanolamine 0.12 Preservative, fragrance, pigment a very small amount Distilled water Balance Sum 100

[ Prescription example  4: nutrition cream]

As one example of cosmetics, nutritional creams containing preparations of barbitum tilandii of Production Examples 1 to 5 were prescribed (Examples 1 to 5), and the prescription examples were as shown in Table 6 below.

Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Production Example 1 5.0 - - - - - - Production Example 2 - 5.0 - - - - - Production Example 3 - - 5.0 - - - - Production Example 4 - - - 5.0 - - - Production Example 5 - - - - 5.0 - - 1,3-butylene glycol - - - - - - 5.0 Pro-type glycerin monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hardened vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaruronate 4.0 Preservative, fragrance, pigment a very small amount Distilled water Balance Sum 100 100 100 100 100 100 100

[ Prescription example  5: Massage  cream]

As an example of a cosmetic preparation, a massage cream containing Preparation Example 1 of tiland extract was prepared. The prescription examples were as shown in Table 7 below.

ingredient Content (unit:% by weight) Beard tillandiae extract 45.0 Pro-type glycerin monostearate 1.5 Stearyl alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl isoserate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanolamine 0.7 Preservative, fragrance, pigment a very small amount Distilled water Balance Sum 100

[ Prescription example  6: Essence]

As an example of the cosmetic, an essence containing the tilia tilia extract of Preparation Example 1 was prescribed, and the prescription examples were as shown in Table 8 below.

ingredient Content (unit:% by weight) Beard tillandiae extract 70.0 glycerin 10.0 Betaine 5.0 PIZZY 1500 2.0 Allantoin 0.1 DL-Panthenol 0.3 Lee D. T.-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl cellulose 0.1 Sodium hyaruronate 8.0 Carboxyvinyl polymer 0.2 Triethanolamine 0.18 Octyldodecanol 0.3 Octyldodec-16 0.4 ethanol 6.0 Preservative, fragrance, pigment a very small amount Distilled water Balance Sum 100

[ Prescription example  7: Pack]

As an example of the cosmetic, a pack containing the tilia tilia extract of Preparation Example 1 was prescribed, and the prescription examples were as shown in Table 9 below.

ingredient Content (unit:% by weight) Beard tillandiae extract 10.0 Polyvinyl alcohol 15.0 Cellulose sword 0.15 glycerin 3.0 PIZZY 1500 2.0 Cyclostristin 0.15 DL - Panthenol 0.4 Allantoin 0.1 Monoammonium glycyrrhizin acid 0.3 Nicotinamide 0.5 ethanol 6.0 FYGEN 40 Hardened castor oil 0.3 Fragrance, coloring, preservative a very small amount Distilled water Balance Sum 100

[ Experimental Example  2: Skin barrier function recovery effect]

In order to examine the skin barrier function recovery effect of the cosmetic composition containing the extract of Beard tillandiae of the present invention, the following experiment was conducted.

Experiments were carried out by using a taping stripping method to damage the skin barrier and measuring the skin water loss (TEWL, transdermal water loss) by evaporator EP1 from servomed (Sweden) to 4.0 g / M 2 / h, the nutritional creams of Examples 1 to 5 and Comparative Examples 1 and 2 containing the inventive hair tilyland extract of the present invention prepared in Prescription Example 4 were applied to an area of 5 cm 2 , The amount of skin moisture loss was measured after 1 hour, 2 hours, 4 hours, and 8 hours, and the degree of decrease was evaluated to evaluate the degree of recovery of the barrier function. At this time, a known lipid mixture (ceramide: 2: 1: 1 mixture of cholesterol: fatty acid) was used as a positive control. The results are shown in the following Table 10, with reference to the time-dependent changes of the skin moisture loss in the initial damaged barrier state as a reference (100%).

sample 0 hours 1 hours 2 hours 4 hours 8 hours Example 1 85 60 31 20 13 Example 2 85 68 36 26 21 Example 3 85 73 47 40 35 Example 4 85 70 39 29 24 Example 5 85 69 41 30 22 Comparative Example 1 85 75 57 55 48 Comparative Example 2 85 68 60 50 46 Control group 85 75 60 58 47

As shown in the results of Table 10, Examples 1 to 5, which contained the extract of Salmonella tilandii, showed excellent skin barrier function recovery as compared with the control and Comparative Examples 1 and 2. As a result, It was confirmed that the skin moisture loss of Example 1 prepared by extraction was the least.

[ Experimental Example  3: Skin Moisture power  Improvement effect]

In order to examine the skin moisturizing effect of the cosmetic composition containing the extract of the present invention, the following experiment was carried out. Twenty-five to twenty-four to twenty patients without skin disease were divided into two groups each consisting of 20 rats per group. The rats were divided into two groups according to Examples 1 to 5 and Comparative Example 1 2 nutritional creams were applied to the face and forehead twice a day for one month.

The skin conductivity was measured in advance using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application and the values were measured at 1 week, 2 weeks, The skin conductivity increase rate (%) was measured and evaluated. The results are shown in Table 11 below.

sample After 1 week after 2 weeks After 4 weeks Example 1 58 67 78 Example 2 57 59 63 Example 3 47 51 54 Example 4 54 57 61 Implementation 5 48 53 59 Comparative Example 1 32 36 42 Comparative Example 2 44 49 51

As can be seen from the results of Table 11, when comparing Examples 1 to 5, which are cosmetic compositions containing the extract of Tailrillia sieboldii of the present invention, Example 1> Example 2> Example 4> Example 3> Example 5 , Respectively. Particularly, in the case of Example 1, the rate of skin conductivity increase was much higher than those of Examples 2 to 5 and Comparative Examples 1 and 2.

In general, the skin conductivity is proportional to the skin moisture content. From the above results, it can be confirmed that the cosmetic composition containing the extract of Salmonella typhimurium extract according to the present invention maintains a high moisture content of the skin as compared with the cosmetic compositions not containing it.

Claims (5)

delete Comprising the step of preparing a hydrolyzed cyan supercritical extract,
Characterized in that the hydrolyzate is extracted under the conditions of an extraction temperature of 60 DEG C and an extraction pressure of 300 bar by using a mixed fluid obtained by mixing carbon monoxide and ethyl acetate in a hydroxymethylated tylanshia extract. A method for producing a cosmetic composition for promoting differentiation and restoring skin barrier function.
delete delete 3. The method of claim 2,
The formulation of the cosmetic composition may be in the form of an aqueous solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Wherein the composition is prepared in a single formulation. The method for promoting the differentiation of dermal keratinocyte and recovering skin barrier function.