KR100803925B1 - Cosmetic Composition for Preventing Skin Aging Comprising Radix glycyrrhizae, Phellodendri Cortex, Radix Cynanchi Wilfordii and Cortex mori Extract Stabilized in Nanoliposome - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging comprising nanoliposomes stabilized with herbal extracts, and more particularly, nanoliposomes stabilized extract selected from the group consisting of licorice, cornus milk, baekshou, lettuce, and combinations thereof. It relates to a cosmetic composition for preventing skin aging comprising as an active ingredient. The cosmetic composition of the present invention prevents skin aging through the improvement of skin wrinkles and the improvement of skin elasticity and the moisturizing ability by promoting the growth of fibroblasts, antioxidant effect, promotion of collagen synthesis. Furthermore, in the cosmetic composition of the present invention, since the herbal extract is contained by the nanoliposomes, the skin penetration effect is greatly increased, and the stability of the formulation is also greatly improved.

Licorice, Cornus, Baekshao, Sangbaekpi, Cosmetics, Nanoliposomes

Description

Cosmetic Composition for Preventing Skin Aging Comprising Radix glycyrrhizae, Phellodendri Cortex, Radix Cynanchi Wilfordii and Cortex mori Extract Stabilized in Nanoliposome}

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition for preventing skin aging, in which an active ingredient of an extract selected from the group consisting of licorice, cornus, baekshou, lettuce, and combinations thereof is stabilized with nanoliposomes.

Healthy skin has active physiological activity of skin cells, and hyaluronic acid, which is essential for the synthesis and collagen of collagen, which plays an important role in enhancing skin elasticity and suppressing wrinkle formation in fibroblasts in the dermis. Synthesis of glycosaminoglycans (GAGS), a glycoprotein that includes hyaluronic acid, is active to give skin elasticity, softness, flexibility and moisture retention. In the epidermis, the proliferation of basal layer cells is active, and the production of binding proteins such as laminin, integrin, and desmosome, which enhance the binding between skin cells, is balanced. Consists of strengthening skin cell tissue to maintain healthy skin.

However, the skin of modern people is exposed to various harmful environments and stresses outside, and suffers various damages. As the skin ages, the skin's physiological activity decreases and the damaged skin's recovery rate slows down, the skin loses elasticity, dryness, wrinkle formation and Various skin aging symptoms such as dullness are encountered.

The physiological changes of the skin by aging include the thinning of the skin's constituents, the epidermis, the dermis, and the subcutaneous tissue, and the change in the lipid composition and content of the lipid barrier, which functions as a skin barrier. There may be various changes such as a sudden decrease in the moisture content of the skin, dry skin, blemishes, freckles and pigmentation. In addition, in recent years, highly reactive free radicals and free radicals generated by the increase in the amount of ultraviolet rays, air pollution, and excessive fatigue and stress of modern people, oxidize or denature biological components (proteins, nucleic acids, membrane lipids, etc.). As a major cause of skin aging, interest in free radicals has increased. Damage caused by free radicals caused by reduction of oxygen, UV irradiation, phagocytosis, etc. in the process of breathing the living body occurs widely in the human body and is recognized as an important theory during the mechanism of aging. Radicals involved in skin aging include hydroxyl radical, superoxide radical, singlet oxygen, hydrogen peroxide (H ₂ O ₂ ), and the like.

The present inventors can suppress skin aging by well eliminating organic free radicals harmful to the skin, and provide a cosmetic that can enhance the synthesis of collagen (Collagen), which plays an important role in enhancing skin elasticity and suppressing wrinkle formation. As a result of the study, it was found that the extracts selected from the group consisting of licorice, cornus, baekshou, baekbaekpi and combinations thereof were stabilized with nanoliposomes to achieve the above-mentioned objectives as cosmetics for preventing skin aging.

Recently, the use of natural ingredients are increasing in cosmetics, which are skin-friendly and environmentally friendly as natural plant ingredients, and many research institutes are actively researching them. However, most herbal ingredients are not only unstable by themselves, but also have a big problem in the stability of the cosmetic when applied to the cosmetic.

Therefore, in order to solve such a problem, not only pharmaceuticals, but also cosmetics and foods can be delivered in a stable manner without destroying the bioactive components, and the biolipoactive nanoliposomes are used that can contain both fat-soluble or water-soluble. Recently, with the development of nano technology, research and development on nano liposomes that can maximize the skin penetration effect is actively progressing.

The present inventors have diligently researched to develop a cosmetic composition for preventing skin aging, that is, skin protection using herbal medicines, and found that licorice, cornus oil, baekshou and lettuce extract have excellent fibroblast proliferation, antioxidant, and collagen synthesis effects. It was. In addition, in order to keep these active ingredients stable in the cosmetic composition, the formulation was stabilized to nanoliposomes and developed an excellent skin penetration enhancer to complete the cosmetic composition for preventing skin aging.

Accordingly, an object of the present invention to provide a cosmetic composition for preventing skin aging comprising nanoliposomes stabilized herbal extract as an active ingredient.

According to an aspect of the present invention, the present invention provides a cosmetic for preventing skin aging comprising nanoliposomes stabilized with an extract selected from the group consisting of licorice, cornus, baekshou, lettuce, and combinations thereof.

The present inventors continued to study the natural extracts to improve the problems of conventional skin anti-aging cosmetic composition exhibiting side effects on the skin. As a result, licorice, cornus, baekshou and cedar extracts promote the synthesis of collagen, a component that gives skin elasticity, promote the proliferation of fibroblasts, which are mainly collagen synthesis, and increase the skin's moisturizing power with antioxidant effect It was confirmed that there are few irritation side effects.

Furthermore, when encapsulation of the above-mentioned herbal extract in nanoliposomes (Encapsulation), it was found that the skin moisturizing power, skin elasticity is improved, the skin penetration effect is greatly increased, and the stability of the formulation is also greatly improved.

Licorice (Radix glycyrrhizae) is a dried root and leaf of the legume licorice, which acts as an anti-inflammatory and anti-allergic agent to strengthen skin immunity.

Cornus is also the fruit of the cornus officinalis of the dogwood of the dogwood, which is slightly warm in nature, tastes sour, and has no poison. Yin (陰) is vigorous, Shinjeong (精) and Shin (腎氣) to see, enhance the sexual function, penis is enlarged and enlarged. It also helps cleanse (精髓) and warm your back and knees. It helps to urinate more often, to help older people to pee at times, and to soothe wind, nose, and deafness. In addition, the content of saponin 15% or more is excellent in the convergence effect, and also has an effect of removing harmful oxygen to improve the skin. In animal experiments, it shows diuretic and blood pressure-lowering effects, and drug decoction has staphylococcus and dysentery inhibitory effects and ascites cancer cells. Increasing myocardial contractility, raising blood pressure, lymphatic cell proliferation in the immune system, and platelet aggregation suppression.
In addition, Baixiu (Radix Cynanchi Wilfordii) is a dried grass root of the sessile of plants and plants and activates skin cells to make skin clear and healthy, and has the function of smoothing skin penetration of active ingredients.

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In addition, Cortex mori is dried mulberry roots of the mulberry plant is excellent in whitening effect, mild antitussive and diuretic effect, decoction shows blood pressure lowering effect. In addition, it shows sedation, analgesic, body temperature lowering, jingyeong, inhibiting the development of Staphylococcus aureus, influenza bacteria, dysentery bacteria.

The herbal extract of the present invention may be obtained using various extraction methods known in the art, and preferably, (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, Propanol, butanol, etc.), (c) the mixed solvent of the lower alcohol and water, (d) acetone, (e) ethyl acetate, (f) chloroform or (g) 1,3-butylene glycol Can be obtained.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent but also herbal extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the herbal extract of the present invention.

The herbal extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

As used herein, the term "nanoliposomes" refers to liposomes having an average particle diameter of 1-100 nm as having the form of conventional liposomes. According to a preferred embodiment of the invention, the average particle diameter of the nanoliposomes is 30-70 nm. When the average particle diameter of the nanoliposomes exceeds 70 nm, the improvement of skin penetration and the improvement of formulation stability are very weak among the technical effects to be achieved in the present invention.

According to a preferred embodiment of the present invention, the herbal extract is contained 0.01-40% by weight, more preferably 0.1-20% by weight, most preferably 4-14% by weight relative to the total weight of nanoliposomes do.

According to a preferred embodiment of the present invention, the nanoliposome is contained 0.0001-20% by weight, more preferably 0.01-15% by weight, most preferably 0.1-10% by weight based on the total weight of the cosmetic.

The nanoliposomes of the present invention are prepared by a mixture comprising polyols, surfactants, phospholipids, fatty acids and water, and are characterized by not using monohydric alcohols (eg ethanol).

The polyol used in the nanoliposome of the present invention is not particularly limited, preferably propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methyl propanediol, isoprene glycol, pentylene glycol, erythritol, Ziitol, sorbitol, and mixtures thereof. It is preferably selected from the group consisting of propylene glycol, 1,3-butylene glycol, glycerin and mixtures thereof, most preferably glycerin. The amount used is 10-80% by weight, preferably 30-70% by weight based on the total weight of the nanoliposomes.

Surfactants used in the preparation of the nanoliposomes of the present invention may be any known in the art, for example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants may be used. , Preferably anionic surfactants and nonionic surfactants are used. Specific examples of anionic surfactants include alkylacylglutamate, alkylphosphates, alkyllactylates, dialkylphosphates and trialkylphosphates. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkylpolyglycosides, polyglyceryl esters and sugar esters. Most preferably, polysorbates belonging to the nonionic surfactant are used. The amount of the surfactant to be used is generally 0.1-10% by weight, preferably 0.5-5.0% by weight based on the total weight of the nanoliposomes.

Phospholipids, another component used in the preparation of the nanoliposomes of the present invention, are used as amphoteric lipids, including natural phospholipids (eg, egg yolk lecithin or soy lecithin, sphingomyelin) and synthetic phospholipids (eg dipalmitoyl). Phosphatidylcholine or hydrogenated lecithin), preferably lecithin. More preferably, the lecithin is naturally occurring unsaturated lecithin or saturated lecithin extracted from soybean or egg yolk. Typically, lecithin derived from nature has 23-95% phosphatidyl choline and 20% or less phosphadidylethanolamine. In preparing the nanoliposomes of the present invention, the amount of lecithin used is 0.5-20% by weight, preferably 2-8% by weight, based on the total weight of the nanoliposomes.

The fatty acids used in the preparation of the nanoliposomes of the present invention are higher fatty acids, preferably saturated or unsaturated fatty acids of C _12-22 alkyl chains such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid. It includes. The amount used is 0.05-3.0 wt% with respect to the total weight of nanoliposomes, preferably 0.1-1.0 wt%.

Water used for the preparation of the nanoliposomes of the present invention is generally deionized distilled water, the amount of which is used is 5.0-40% by weight relative to the total weight of nanoliposomes.

The preparation of nanoliposomes can be accomplished through various methods known in the art, but most preferably, a mixture comprising the above components is applied to a high pressure homogenizer. The preparation of nanoliposomes by high pressure homogenizer can be carried out under various conditions (eg pressure, frequency, etc.) depending on the desired particle size, preferably 1-5 times high pressure homogenizer under 600-1200 bar pressure. Nanoliposomes are prepared by passing through.

The nanoliposome system thus prepared has the advantage of maximizing skin penetration effect by dissolving various types of poorly soluble substances and stabilizing unstable substances.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to nanoliposomes containing herbal extracts as active ingredients, and include conventional auxiliaries such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The cosmetic composition for protecting the skin of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants- It may be formulated as containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention has the use of skin anti-aging (skin protection). As used herein, the term "anti-aging of skin" has the meaning encompassing comprehensively the prevention and treatment of skin aging. Also, in this specification, "anti-aging of skin" and "skin protection" are used in the same sense unless otherwise specified.

The cosmetic composition of the present invention prevents skin aging through the improvement of skin wrinkles and the improvement of skin elasticity, the increase of antioxidant activity, and the moisturizing ability by promoting the proliferation of fibroblasts, the promotion of collagen synthesis, and this fact is specified in the following examples. have. Therefore, skin aging prevention (ie, skin protection), which is the use of the present invention, is interpreted to include the use of wrinkle improvement, elasticity improvement, moisturizing improvement and skin antioxidant ability improvement. Furthermore, in the cosmetic composition of the present invention, since the herbal extract is contained by the nanoliposomes, the skin penetration effect is greatly increased, and the stability of the formulation is also greatly improved.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those of ordinary skill in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Preparation Example 1: Obtaining a Herb Extract Using Various Extraction Solvents

Preparation Example 1-a

20 g each of licorice, cornus oil, white sewage and lettuce were washed with purified water, dried and 20 g of powdered powder was added to 1.5 L of a 70% ethanol solution and heated and extracted for 4 hours in a reflux extractor equipped with a cooling condenser. . Then, the obtained extract was filtered with a 300 mesh filter cloth of Wattman filter paper, the residue was extracted once more in the same manner, and the respective extracts were combined and filtered again with filter paper of Wattman No. 5, and the solvent was removed using a vacuum concentrator. Removed and lyophilized to give an extract of 4.89 g dry weight.

Preparation Example 1-b

20 g each of licorice, cornus oil, white sewage and baekbaekpi were washed with purified water and dried, and then 20 g of powdered powder was added to 1.5 l of 1,3-butylene glycol solution, and the same method as in Preparation Example 1-a was performed. To give a dry weight of 4.01 g of an extract.

Preparation Example 1-c

20 g each of licorice, cornus oil, white sewage and baekbaekpi were washed with purified water and dried. Then, 20 g of powdered powder was added to 1.5 L of acetone, and the same procedure as in Preparation Example 1-a was carried out to obtain a dry weight of 4.72 g of extract. Obtained.

Preparation Example 1-d

20 g each of licorice, cornus oil, white sewage and baekbaekpi were washed with purified water and dried. Then, 20 g of powdered powder was added to 1.5 l of purified water, and the same procedure as in Preparation Example 1-a was carried out to extract a dry weight of 1.32 g. Obtained.

Preparation Example 1-e

20 g of licorice, cornus oil, white sewage and baekbaekpi were washed with purified water and dried. Then, 20 g of powdered powder was added to 1.5 L of 70% methanol and dried in the same manner as in Preparation Example 1-a, to obtain a dry weight of 4.19 g. An extract of was obtained.

Preparation Example 1-f

20 g each of licorice, cornus oil, white sewage and baekbaekpi were washed with purified water and dried. Then, 20 g of powdered powder was added to 1.5 L of ethyl acetate solution and dried in the same manner as in Preparation Example 1-a. An extract of was obtained.

Preparation Example 1-g

20 g each of licorice, cornus oil, white sewage and baekbaekpi were washed with purified water and dried. Then, 20 g of powdered powder was added to 1.5 L of chloroform solution, and the same method as in Preparation Example 1-a, An extract was obtained.

Preparation Example 1-h

20 g each of licorice, cornus oil, white sewage and baekbaekpi was washed with purified water and dried, and then 20 g of powdered powder was added to 1.5 L of a normal butanol solution, followed by the same method as Preparation Example 1-a to give a dry weight of 3.77 g An extract was obtained.

Experimental Example 1 Fibroblast Proliferation Effect

Human normal fibroblasts (Korea Cell Line Bank) are seeded to 1 x 10 ⁴ cells in each well of a 96-well microplate and incubated at 37 ° C. for 24 hours in DMEM medium (purchased by sigma). It was. Subsequently, each experimental herbal extract prepared in Examples 1-a to 1-h was replaced with serum-free DMEM medium containing a final concentration of 100 μg / ml and serum-free DMEM medium without herbal extracts Controls replaced with were further incubated for 24 hours. Then, 10 μl of MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added to each, and incubated for 4 hours. The medium was then removed, shaken for 20 minutes by addition of 100 μl of dimethyl sulfoxide solution per well and the absorbance of the supernatant was measured at 570 nm using a microplate reader (Molecular Devices, USA). . The measured values were substituted into the following Equation 1 to calculate the cell proliferation effect, and the results are shown in Table 1 below.

extract Absorbance Cell proliferation effect (%) Control 0.325 - Preparation Example 1-a 0.501 41.6 Preparation Example 1-b 0.476 30.3 Preparation Example 1-c 0.485 35.4 Preparation Example 1-d 0.445 21.1 Preparation Example 1-e 0.484 34.3 Preparation Example 1-f 0.431 25.3 Preparation Example 1-g 0.496 38.6 Preparation Example 1-h 0.431 24.2

As can be seen in Table 1, it can be seen that the present invention shows a fibroblast proliferation effect of about 20-42% at 100 ㎍ / ㎖ concentration of the extract compared to the control not treated with the extract.

Experimental Example 2: Effect of Cell Proliferation According to the Concentration of Herbal Extracts

In Example 1, the extract prepared in Preparation Example 1-a, which was found to have the best fibroblast proliferation effect, was measured at a concentration of 50, 100 or 200 ㎍ / ml, and the cell proliferation effect was measured in the same manner as in Experiment 1. The results are shown in Table 2 below.

Concentration (μg / ml) Absorbance (570 nm) Cell proliferation effect (%) Control 0.325 - 50 0.425 32.46 100 0.474 41.57 200 0.521 57.22

As can be seen in Table 2, it can be seen that the extract promotes the growth of fibroblasts in a concentration-dependent manner, and it can be confirmed that the results in Experimental Example 1 are significant.

Experimental Example 3: Collagen Synthesis Enhancement Effect

Human normal fibroblasts (Korea Cell Line Bank) were seeded in each well of a 96-well microplate to be 2 × 10 ⁴ cells and incubated for 24 hours at 37 ° C. in DMEM medium. Subsequently, the herbal extract prepared in Example 1-a was replaced with a serum-free DMEM medium containing a serum-free DMEM medium containing a final concentration of 50, 100 or 200 μg / ml and a serum-free DMEM medium containing no herbal extracts. One control was further incubated for 24 hours, ascorbic acid (50 g / ml) was added to promote collagen synthesis, followed by another 24 hours. Each well was then washed and replaced with serum-free DMEM medium and incubated for an additional 24 hours. After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured using a kit (Takara, Japan) for the amount of procollagen (procollagen) type I C-peptide (PICP). PICP amount was converted to ng / 2 × 10 ⁴ cells, and the results are shown in Table 3 below.

Concentration (μg / ml) Collagen Synthesis (ng / 2 x 10 ⁴ ) Control 154 50 187 100 205 200 248

As can be seen in Table 3, it can be seen that the synthesis of collagen increases as the concentration of the herbal extract increases, and thus it can be seen that the herbal extract shows an excellent collagen synthesis promoting effect.

Experimental Example 4: Antioxidant Effect

Experimental Example 4-1: Lipid Peroxidation Experiment

The fenton reaction of antioxidant activity against the herbal extract prepared in 50, 100 or 200 μg / ml of the extract prepared in Preparation Example 1-a, which was confirmed to have the best fibroblast proliferation effect in Experimental Example 1 It was measured by thiobarbituric acid method (TBA assay) using a lipid peroxidation system.

Dimethylsulfoxide of the herbal extract in 5 ml of 25 mM Trizma hydrochloric acid / 0.75 mM potassium chloride buffer containing 10 μl of ethyl linoleate, 0.2% sodium dodecyl sulfoxide (SDS), 10 μmol of ferrous chloride, and 2 μmol of hydrogen peroxide. DMSO) After adding the test solution and incubating at 55 ° C. for 16 hours, 50 μl of 4% dibutylhydroxytoluene (BHT) is added to stop the reaction. 0.3 ml of this solution is added to a test tube, and 0.05 ml hydrochloric acid 3.0 ml and 0.67% thiobarbituric acid (TBA) solution are added and boiled for 30 minutes. After cooling the reaction solution, 4.0 ml of 85% butanol was added thereto, and the absorbance of the butanol layer was measured at 535 nm after centrifugation.

The antioxidant effect of each extract was calculated by the following equation. The results are shown in Table 4 below.

extract Antioxidant activity (%) Experimental concentration (㎍ / ㎖) ^* 10 50 100 Control 5 5 5 Preparation Example 1-a 48 62 83 Preparation Example 1-b 47 59 82 Preparation Example 1-c 41 56 73 Preparation Example 1-d 19 52 69 Preparation Example 1-e 40 63 80 Preparation Example 1-f 25 49 81 Preparation Example 1-g 42 60 79 Preparation Example 1-h 26 50 77

* 3 times average

Most herbal extracts showed high activity, which was more effective in extracts of polar solvents than nonpolar solvents.

Experimental Example 4-2: Free Radical Scavenging Experiment

Free radical DPPH (1,1-diphenyl-2-picryl hydrazyl) was used as Sigma. Since DPPH is purple as a stable free radical, it is a measure of the extent to which purple is removed as the sample eliminates radicals. First, DPPH was dissolved in methanol at a concentration of 1.5 × 10 ⁻⁴ M and 500 μl of this solution was placed in a test tube. The extract of each concentration was dissolved in methanol, 500 µl was added thereto, and the absorbance was measured at 517 nm after reaction at room temperature for 30 minutes. In this case, when the extract was not dissolved in methanol, dimethyl sulfoxide: methanol = 4: 1 solvent was used. Ascolic acid was used instead of the extract of Example as a control. The free radical scavenging effect of each extract was expressed as EC ₅₀ by obtaining a concentration of 50% scavenging of radicals by using Equation 3 below. The results are shown in Table 5 below.

extract Free radical scavenging ability (%) Experimental concentration (㎍ / ㎖) 10 50 100 Ascoville Mountain 10.5 10.5 10.5 Preparation Example 1-a 18 46 67 Preparation Example 1-b 16 42 66 Preparation Example 1-c 15 39 62 Preparation Example 1-d 11 30 51 Preparation Example 1-e 13 35 57 Preparation Example 1-f 15 37 61 Preparation Example 1-g 16 41 63 Preparation Example 1-h 15 38 60

As shown in Table 5, the herbal extract of the present invention showed a potent activity compared to ascorbic acid (vitamin C), a powerful antioxidant.

Experimental Example 5: Preparation of nanoliposomes containing herbal extracts

The nanoliposomes were added to phase A in the composition of Table 6 below, wetted uniformly, and then phase C was added and heated and mixed to 70-80 ° C. The solution was passed through a high-pressure homogenizer 2-4 times under conditions of 600-1000 bar and cooled to obtain nanoliposomes in which herbal extracts having a diameter of 30-70 nm were stabilized.

Prize ingredient Content (% by weight) A Propylene glycol 40 B lecithin 5 cholesterol 2.5 Ceramide 2.5 Sodium Stearoyl Lactate 0.5 Polysorbate-80 0.5 Stearic acid 0.5 C Herbal Extract (Solid) 10 Potassium hydroxide Quantity Purified water To 100

Examples and Comparative Examples

Compositions containing nanoliposomes stabilized with the herbal extracts of the present invention (examples) and compositions without nanoliposomes (comparative examples) were prepared in the following compositions.

ingredient Example Comparative Example Content (% by weight) Content (% by weight) Nanoliposomes (Table 6) 10 - Herbal Extract (Solid) - 10 A Cetostearyl alcohol 2.0 2.0 Glyceryl Stearate 1.5 1.5 Mikey-Crystalline 0.7 0.7 Squalane 5.0 5.0 Liquid paraffin 3.0 3.0 Trioctanoine 5.0 5.0 Polysorbate 1.2 1.2 Sorbitan stearate 0.5 0.5 Tocopherol Acetate 0.2 0.2 Micromethicone 3.0 3.0 BHT 0.05 0.05 B glycerin 4.0 4.0 1,3-butylene glycol 2.0 2.0 EDTA-2Na 0.05 0.05 Purified water To 100 To 100 Incense, preservative Quantity Quantity

Experimental Example 6: Moisturizing Effect

In order to evaluate the moisturizing power of each cosmetic prepared in the above Examples and Comparative Examples, the moisture retention capacity and the water evaporation amount of the skin to which each cosmetic was applied were measured as follows.

Experimental Example 6-1: Measurement of Water Retention Capacity

In the constant temperature and humidity room of 22 ° C. and 45% RH, each cosmetic prepared in Examples and Comparative Examples was coated with a predetermined amount (0.03 g / 16 cm 2) in the inner half of 30 test subjects, before application and after 1 hour of application. And moisture content of the skin 2 hours after application was measured, and normal skin without any treatment was used as a control. As a measuring device, a moisture content meter (corneometer CM820, Conrage + Khazaka, Germany) was used to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 6 below.

division Example Comparative Example Control Skin electrical conductivity before application 50 50 50 Skin electrical conductivity 1 hour after application 87 74 50 Skin electrical conductivity 2 hours after application 76 63 50

Experimental Example 6-2: Measurement of Water Evaporation Amount

After tape stripping on the upper arm of the arm to weaken the skin's protective film, apply the cosmetics to the subject in the same manner as in Experiment 4-1, and then use a moisture evaporator (TEWA meter, koln, Germany). Federated water evaporation was measured 15 times in 1 hour, 2 hours, 4 hours, 6 hours, and 8 hours, and the average value was obtained. The results are shown in Table 9 below.

division Example Comparative Example Control Moisture evaporation after 0 hours 3.0% 3.0% 0.0% Moisture evaporation after 1 hour -8.7% -4.6% -0.3% Water evaporation after 2 hours -7.5% -0.8% 0.1% Water evaporation after 4 hours -4.6%  2.2% 0.1% Water evaporation after 6 hours -2.1%  2.8% -0.2% Evaporation after 8 hours -1.5% 3.1% 0.0%

As shown in the results of Experimental Examples 6-1 and 6-2, the cosmetics of the examples of the present invention have higher electrical conductivity of the skin and less water loss than the cosmetics of the comparative examples. Therefore, it can be seen that the cosmetic of the present invention exhibits an excellent moisturizing effect.

Experimental Example 7: Skin Elasticity Effect

The skin elasticity enhancing effect of the cosmetic composition containing the nanoliposome stabilized herbal extract of the present invention was measured.

Thirty healthy women (mean age 38 years old) over 20 years old (average age 38 years) were divided into two groups under the conditions of temperature 24-26 ° C and humidity 75. After 12 weeks of application (morning and evening), skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germary). The test results are described as R8 (R8 (12 weeks) -R8 (0 weeks)) values of cutometer SEM 575, where R8 values indicate the nature of the viscoelasticity, and the results are shown in Table 10 below.

Experimental Formulation Skin elasticity effect Example 0.68 Comparative Example 0.34

As shown in the results of Table 10, the skin elasticity of the examples containing the herbal extracts stabilized with nanoliposomes increased by two times compared to the comparative example used in the formulation directly without stabilizing the herbal extracts. Therefore, it can be seen that the embodiment containing the nanoliposomes stabilized herbal extract shows a much better effect on skin elasticity.

Experimental Example 7: Skin Penetration Effect

The skin penetration effect on the cosmetic containing nanoliposomes stabilized herbal extract of the present invention was measured.

Dermal Equivalent (DE) -human normal fibroblasts 1 X 10 ⁵ cells / mL in a gel structure similar to that of human connective tissue 3 mg / mL: 5 X DEM: 2: 2 sodium bicarbonate and 200 0.05 N sodium hydroxide containing mM Hepes buffer was inoculated in a medium mixed with 7: 2: 1 and incubated at 5CO ₂ , 37 ° C for 7 days. It was placed inside a plate separated from the inside and the outside by a membrane made of a 3 μm porous polycarbonate material, and epidermal Keratinocyte on the surface of Derma Equivalent (DE) where fibroblasts were cultured. ) Inoculated with 1 X 10 ⁵ cell / ㎖ cells and cultured for 7 days in the inner and outer K-SFM medium containing EGF and BPE. Afterwards, the medium inside the plate was discarded to allow the air to come into contact with the surface of the cultured cells. The medium was mixed with K-SFM containing 10FBS and DMEM without EGF in the same amount and incubated for 2 weeks. Artificial skin with dermis was obtained. The experimental and comparative examples of the present invention were applied to the artificial skin tissue of the top layer, and after 4 hours of incubation, the amounts of 10-FBS-containing K-SFM and water were analyzed by HPLC. The test results are shown in Table 11 below.

Amount of herbal extract in the medium (µg / ml) Example Comparative Example 40.5 2.9

Experimental results As shown in Table 11, the cosmetic containing the herbal extracts stabilized with the nanoliposomes of the present invention can be seen that the skin penetration effect is more than 10 times better than the comparative example using the herbal extracts as it is. This is because the average particle size of the nanoliposome of the present invention is very small, 30-70 nm, so that skin penetration is easy. Therefore, in the above-mentioned skin elasticity enhancing effect of Experimental Example 7, it can be seen that the embodiment of the present invention exhibits an excellent skin elasticity enhancing effect because the skin penetration effect is superior to the comparative example.

Experimental Example 8: Skin irritation test

In order to examine the skin irritation of the herbal extract prepared according to the preparation example, a certain amount (about 0.1 g) of each sample was patched for 24 hours on 5x20 cm in the lower part of both arms for 30 healthy adults males and females. Then, after 1 hour and 24 hours after removal, the skin condition change was read visually by the following criteria, and the stimulation rate was calculated according to the following equation (4). The experimental results are shown in Table 12 below.

Criteria

-: No erythema or unusual phenomenon

+-: Slightly reddish

+: Significantly redder than the surroundings

++: Severe reddening and swelling around

Production Example Judgment result Stimulation rate (%) ++ + +- - 1-a - - 2 28 2.2 1-b - - One 29 1.1 1-c - - 2 28 2.2 1-d - - 2 28 2.2 1-e - - One 29 1.1 1-f - - 3 27 3.3 1-g - - 3 27 3.3 1-h - - One 29 1.1

As can be seen from the skin irritation test results of Table 12, the extract of the present invention can be seen that there is no skin irritation.

Hereinafter, based on the results of the experimental example described above, a cosmetic composition containing the herbal extract stabilized with the nanoliposome of the present invention is presented. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1: Softener (Skin Lotion)

As shown in the following table, the softening lotion was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Nanoliposomes 1.0 1.3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 2. Nutrients (Milk Lotion)

Nutrients were prepared according to a conventional method as shown in the table below.

Compounding ingredients Content (% by weight) Nanoliposomes 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Formulation Example 3. Nutrition Cream

Nutritious cream was prepared according to a conventional method as shown in the following table.

Compounding ingredients Content (% by weight) Nanoliposomes 10.0 Cetostearyl alcohol 2.0 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.1 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 4 Massage Cream

Massage cream was prepared according to a conventional method as shown in the table below.

Compounding ingredients Content (% by weight) Nanoliposomes 1.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation Example 5 Pack

Packs were prepared according to conventional methods as shown in the table below.

Compounding ingredients Content (% by weight) Nanoliposomes 2.0 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

As described above, the present invention provides a cosmetic composition for preventing skin aging comprising nanoliposomes containing an extract selected from the group consisting of licorice, yellowish white, baekshou, baekbaekpi and combinations thereof as an active ingredient. The cosmetic composition of the present invention prevents skin aging through the improvement of skin wrinkles and the improvement of skin elasticity, the moisturizing ability and the antioxidant effect by promoting the growth of fibroblasts, the promotion of collagen synthesis. Furthermore, in the cosmetic composition of the present invention, since the herbal extract is contained by the nanoliposomes, the skin penetration effect is greatly increased, and the stability of the formulation is also greatly improved.

Claims (6)

A cosmetic composition for preventing skin aging comprising nanoliposomes stabilized with herbal extracts consisting of licorice, cornus, baekshou and baekbaekpi as active ingredients. The herbal extract of claim 1, wherein the herbal extract comprises (a) water, (b) anhydrous or hydrous lower alcohol having 1-4 carbon atoms, (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1,3 A cosmetic composition for preventing skin aging, characterized in that obtained by using butylene glycol as an extraction solvent. The cosmetic composition for preventing skin aging according to claim 1, wherein the herbal extract is contained in an amount of 0.01-40% by weight based on the total weight of nanoliposomes. The cosmetic composition according to claim 1, wherein the cosmetic composition comprises 0.0001-20% by weight of the nanoliposome with respect to the total weight of the cosmetic. The cosmetic composition for preventing skin aging according to any one of claims 1 to 4, wherein the nanoliposomes are prepared by a mixture comprising a polyol, a surfactant, a phospholipid, a fatty acid, and water. The composition of claim 1 wherein the cosmetic composition consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Cosmetic composition for preventing skin aging, characterized in that it has a formulation selected from the group.