KR20130068320A - Cosmetic composition containing ligustrum lucidum aiton extract for moisturizing the skin - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a Ligustrum lucidum Aiton extract as an active ingredient for moisturization is provided to recover damaged skin barrier function and to enhance skin moisturization. CONSTITUTION: A cosmetic composition for moisturization contains 0.001-50wt% of a Ligustrum lucidum Aiton extract as an active ingredient. The composition is used for strengthening skin barrier function and inducing keratinocyte differentiation.

Description

Moisturizing Cosmetic Composition Containing Jeju Orchard Extracts {{Cosmetic composition containing Ligustrum lucidum Aiton extract for moisturizing the skin}

The present invention relates to a moisturizing cosmetic composition, and more particularly, to a cosmetic composition having an excellent effect of restoring damaged skin barrier function and increasing skin moisturizing power by containing the extract of Ligustrum lucidum Aiton as an active ingredient.

Skin plays a very important role as a barrier to protect individuals from the outside. The barrier function is to protect against various stimuli (chemicals, air pollutants, dry environment, ultraviolet rays, etc.) from the outside and to prevent excessive release of moisture through the skin, and this protection function is a stratum corneum composed of keratinocytes. The function can be maintained only when is normally formed.

The outermost stratum corneum (horney layer) is formed from keratinocytes and consists of the differentiated keratinocytes and the surrounding lipid layer (Marcelo CL et al, J. Invest . Dermatol . 80 , pp 37-44, 1983).

Keratinocytes are characteristic cells whose basal cells, which continuously proliferate in the epidermal layer, undergo morphological and functional changes in stages and rise to the surface of the skin. Exfoliated and new keratinocytes take over their function. This repetitive sequence of changes is called "epidermal differentiation" or "keratinization."

In addition, during keratinization, keratinocytes form a natural moisturizing factor (NMF) and intercellular fats (ceramides, cholesterol, fatty acids), forming a stratum corneum, which makes the stratum corneum firm and flexible, thus creating a skin barrier. function as a barrier.

The stratum corneum can easily lose its function due to excessive face washing, lifestyle factors such as bathing, environmental factors such as dry air pollutants, and endogenous diseases such as atopic or aged skin. Recently, due to a variety of factors, more and more skin complaints and dry skin complaints are increasing in recent years.

Therefore, various studies have been conducted to supply moisture from the outside to prevent proper skin moisture or to prevent moisture loss from the body, and various moisturizers having water retention capabilities have been developed. As skin moisturizers, it is common to use lipid components such as ceramides and substances that can increase water retention in the stratum corneum such as essential fatty acids and lipid complexes (Rawlings AV et al, J. Invest . Dermatol. , 5, pp731- 741, 1994).

However, in recent years, the risk factors for the skin are gradually increasing, and the changes in dietary patterns slow down the formation and dropping rate of the stratum corneum, and the amount of moisturizing factors and lipids in the stratum corneum decreases due to the deterioration of the function of keratinocytes. There is a growing number of people with skin that do not function as a skin barrier. This disruption of skin barrier function causes various skin diseases such as dry skin, atopic dermatitis, contact dermatitis, and psoriasis. These diseases can be expected to alleviate symptoms only with a conventional moisturizer having a water retention function, but fundamental healing is difficult.

Jeju Ligustrum lucidum Aiton) is also known as dangwang tree, a kind of wild tree native to Jeju Island. Leaves are large, leathery, broad ovate, broad oval or ovate long oval, acute or obtuse, original or basal, 3-10cm long, 2.5-4.5cm wide, with insignificant residues on the back and flat edges. 5-12mm and reddish brown with leaf veins. Flowers bloom in July-August, flowers are white, abdominal flowers hang at the end of the branch, and the length and width are 5-12cm, respectively, and the calyx is flat or wavy at the edge, the corolla is 5-6mm long, and the tube is slightly longer or the same as the lobe. It is flipped backward and there are two surgeries. Fruits mature in October, and the nucleus is ovate round, 7-10mm long, ripened in purple and remains in winter. Branches have fine hairs, dense leaves, egg-shaped oval or slightly round, short in leaf disease, inflorescences with short hairs on the axial axis.

The mature fruit of the oak tree is called Journey Room. When the fruit matures in the winter, the leaf is removed and dried in the sun, or the fruit is lightly distilled and dried in the sun. Journey room is active against cardiac, diuretic and hepatic function, promotes blast transformation of healthy people, staphylococcus aureus and dysentery bacillus, influenza bacteria, Pseudomonas aeruginosa, E. coli, and anticancer effect. The roots of the mineral wood and the sugar-mined tree are called Jook-geun and are harvested from September to October. It is effective to disperse blood and stop the pain. Bark of ore and sugar tree is called yeast bark and is collected frequently throughout the year, contains large amount of syringic acid, and is known to have antimalarial and antipyretic effects. The leaves of the orchard and the sugar tree are syringic acid, amygdaline degrading enzyme, imvertase, mannitol, ursolic acid, oleanolic acid, p -hydrophenylethanol, cosmosiin, luteolin-7-glucoside, etc. It has been found to have antimicrobial activity against Pseudomonas aeruginosa and Escherichia coli, but there has been no report on the skin barrier reinforcing function or moisturizing effect.

The inventors of the present invention while searching to find a material that exhibits an excellent skin moisturizing effect among natural extracts, Jeju light ( Ligustrum lucidum Aiton) extract was confirmed to have an excellent effect of restoring the damaged skin barrier function and increase the amount of skin moisturizing to complete the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic composition excellent in containing the extract of jeju light wood as an active ingredient and excellent skin moisturizing effect and damaged skin barrier.

In order to achieve the above object, the present invention provides a moisturizing cosmetic composition containing the extract jeju tree as an active ingredient.

Jeju tree extract of the present invention has the effect of increasing the repair and repair of damaged skin barrier function. Therefore, the cosmetic composition containing the extract jeju yeast tree is useful for the prevention or improvement of skin dryness, atopic dermatitis, contact dermatitis or psoriasis as a composition for skin moisturizing, skin barrier function and induction of keratinocyte differentiation. Can be used.

The cosmetic composition of the present invention is Jeju light ( Ligustrum lucidum Aiton) extract as an active ingredient.

Jeju tree extract used in the present invention is not only a leaching solution obtained by leaching and transferring from Jeju tree, but also a concentrate obtained by partially or fully condensing the leaching liquid or a stagnation, premises, regular, flow extract prepared by drying the above concentrate again. And the chemicals contained in the Jeju tree and exert their main effects, as well as the plants themselves. In addition, in the present invention, extracts from all parts of the jeju tree, such as stems, roots, leaves, flowers, and fruits, can be used, and are not limited to extracts of any particular part.

It is possible to use a well-known method as a manufacturing method of Jeju tree extract used in the present invention. For example, the jeju ore wood of the present invention is chopped and dried, and then water or an organic solvent is added, reflux extraction is performed, and the residue and the filtrate are separated by filtration and centrifugation, and the filtrate is concentrated under reduced pressure. You can get it. The organic solvent usable in the present invention may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water, and considering the safety of the raw material, preferably water, 70% ethanol or butyl Butylene glycol is used. At this time, the extraction temperature is preferably 18 to 80 ℃ for water, 18 to 40 ℃ is preferable for 70% ethanol and butylene glycol, the extraction time is preferably 6 to 168 hours. If the extraction temperature and the extraction time is out of the extraction efficiency may be lowered or a change of components may occur. After obtaining the extract using the solvent in the above can be obtained by cooling, heating and filtration at room temperature in a conventional manner known in the art to obtain a liquid, or may further evaporate the solvent, spray drying or freeze drying.

The external preparation composition for skin according to the present invention may contain the jeju tree extract in an amount of 0.001 to 50% by weight based on the total weight of the composition. If the content of the jeju tree extract is less than 0.001% by weight, the efficacy and effect of the extract is weak, and if the content exceeds 50% by weight is due to skin safety or formulation problems.

 The moisturizing cosmetic composition according to the present invention can be used for enhancing skin barrier function and inducing differentiation of keratinocytes of the skin, thus preventing or improving skin dryness, atopic dermatitis, contact dermatitis or psoriasis, etc., which are caused by incomplete epidermal differentiation. It can be usefully used as a topical skin composition.

Cosmetic compositions according to the invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) and non- It may be provided in the form of an ionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or cone stick. It can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

In addition, the cosmetic composition according to the present invention is a fatty substance, an organic solvent, a dissolving agent, a thickening agent, a gelling agent, a softener, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic type or a non- With ionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in cosmetics. It may contain adjuvants commonly used in the same cosmetic or dermatology field. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

In addition, the cosmetic composition of the present invention may contain a skin absorption promoting substance to increase the skin moisturizing effect, skin barrier function strengthening effect, and skin keratinocyte differentiation inducing effect.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to test examples and examples. However, these test examples and examples are provided only for the purpose of illustration in order to help the understanding of the present invention, but the scope and scope of the present invention is not limited by the following examples.

[ Reference Example  1] Preparation of Jeju Tree Extract

1 kg of the dried Jeju leaves were prepared as a 5 mesh powder through a grinder, and then added to 10 L of purified water, and then connected to a reflux apparatus, and heated to reflux for 24 hours. After removing the solids using an 80 mesh sieve, the remaining filtrate was filtered again, and then the solvent in the filtrate was distilled off using a vacuum concentrator to obtain 129 g of jeju tree extract.

[ Test Example  1] Measurement of keratinocyte differentiation promoting effect

In order to examine the differentiation promoting effect of keratinocytes of the Jeju tree extract obtained from Reference Example 1, the amount of CE (Cornified Envelop) generated during the differentiation of keratinocytes was measured using absorbance.

First, the keratinocytes of the primary cultured humans isolated from the epidermis of the newborn were put in a culture flask and attached to the bottom, and then the test substance of Table 1 was treated at 5 ppm in the culture medium, and then the cells were 70 ~ of the floor area. It was incubated for 5 days until it grew about 80%. At this time, the low calcium (0.03mM) treated group and the high calcium (1.2mM) treated group were used as negative and positive controls, respectively. Then, the cultured cells were harvested and washed with PBS (Phophate buffered saline), followed by 10 mM Tris-HCl buffer (Tris-HCl, containing 2% SDS (sodium dodecyl sulfate) and 20 mM Dithiothreitol (DTT). pH 7.4) 1 ml was added to sonication, boiling and centrifugation, and the precipitate was suspended in 1 ml of PBS again to measure absorbance at 340 nm. Separately, a portion of the solution after the sonication was taken to measure the protein content and used as a reference when evaluating the degree of cell differentiation. The results are shown in Table 1 below.

Test substance The ability to differentiate in keratinocytes (%) Low Calcium (0.03 mM) Solution
(Negative control) 100 High calcium (1.2 mM) solution
(Positive control group) 210 Reference Example 1 165

As shown in Table 1, it was confirmed that the treatment of Reference Example 1, which is Jeju jeju extract, is excellent in promoting the differentiation of keratinocytes.

[ Test Example  2] Skin barrier function recovery effect measurement

In order to measure the effect of the Jeju tree extract on the recovery of damaged skin barrier function due to skin damage, the following experiment was performed. Ten adult men and women's upper and lower limbs were damaged by a tape stripping method for 7 days while applying two groups of Formulation Example 1 and Comparative Formulation Example 1, each of which was prepared with the composition shown in Table 2 below. The recovery of transdermal moisture loss (TWEL) was measured once a day using a Vapometer (Delfin, Finland). Here, Formulation Example 1 is a composition containing Reference Example 1, and Comparative Formulation Example 1 is a negative control group (vehicle). The experimental results are shown in Table 3 below. The results in Table 3 were compared on the basis of the 100% difference before and after barrier damage.

Compounding ingredient Formulation Example 1 Comparative Formulation Example 1 Purified water 69 70 Propylene glycol 30 30 Reference Example 1 One -

Test group TWEL change (%) Before processing 1 day 2 days 3 days 4 days 5 days 6 days Formulation Example 1 100 103 84 57 33 26 12 Comparative Formulation Example 1 100 121 112 98 70 62 43

As can be seen in Table 3, when treating the formulation example 1 containing the jeju tree extract, it can be seen that the transdermal moisture loss returns to normal at a rapid rate and the barrier damage is recovered.

[ Test Example  3] skin Moisturizing  Increase effect measurement

In order to measure the effect of the Jeju tree extract on the skin moisturizing power, using the Jeju tree extract obtained in Reference Example 1 according to the composition ratio of the following Table 4 as an external preparation as a formulation of nutritional cream Formulation Example 2 and Comparative Formulation Example 2 was prepared. The unit of the content ratio of the following ingredients is% by weight.

Compounding ingredient Formulation Example 2 Comparative Formulation Example 2 Purified water To 100 To 100 Reference Example 1 One - Vegetable hydrogenated oil 1.50 1.50 Stearic acid 0.60 0.60 Glycerol stearate 1.00 1.00 Stearyl alcohol 2.00 2.00 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.00 1.00 Arakidil Behenyl Alcohol & Arakidil Glucoside 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 PEG-100 Stearate & Glycerolate & Propylene Glycol 1.50 1.50 Caprylic / Carric Triglycerides 11.00 11.00 Cyclomedicon 6.00 6.00 Preservative, incense Suitable amount Suitable amount Triethanolamine 0.1 0.1

In order to confirm the skin moisturizing improvement effect of Formulation Example 2 and Comparative Formulation Example 2 of Table 4 was evaluated as follows. Sixty men and women in their 40s and 50s, classified as dry skin, were divided into two groups of 30 people for two groups of Formulation Example 2 and Comparative Formulation Example 2, respectively. Skin moisture meter at constant temperature and humidity conditions (24 ° C, 40% relative humidity) before start of application, after 1 week, 2 weeks, 4 weeks after application, and after 2 weeks (total 6 weeks) after application is stopped. Skin moisture content was measured by (Corneometer CM825, C + K Electronic Co., Germany). The results are shown in Table 5 below. The results in Table 5 show the percentage increase of the measured value after a certain period of treatment based on the skin moisture meter value measured immediately before the start of the test.

Test group Moisture growth rate (%) 1 week Two weeks 4 weeks 6 weeks Formulation Example 2 31 33 35 30 Comparative Formulation Example 2 30 32 32 15

In the results of Table 5, when the Comparative Formulation Example 2 was applied, it showed a water increase rate of about 30% up to 4 weeks after the application, but after stopping the application, the moisture content of the skin decreased, whereas In the case of coating containing Formulation Example 2, it can be seen that the skin moisture increase rate is more than 30% even after the application is stopped. Through this, it can be seen that the composition of the present invention containing jeju light extract is excellent in skin moisturizing effect.

From the above results, it can be seen that the extract of Jeju tree is very excellent in promoting the differentiation of keratinocytes, restoring damaged skin barrier and increasing skin moisturizing power.

Claims (4)

Jeju Ligustrum lucidum Aiton) Moisturizing cosmetic composition containing the extract as an active ingredient. According to claim 1, wherein the composition is a cosmetic composition, characterized in that for enhancing the skin barrier function. According to claim 1, wherein the composition is a cosmetic composition, characterized in that for inducing differentiation of the keratinocytes of the skin. The cosmetic composition according to any one of claims 1 to 3, wherein the extract of Jeju tree is contained in an amount of 0.001 to 50% by weight based on the total weight of the composition.