KR20200024616A - Cosmetic composition for protecting of skin comprising extract of paeonia lactiflora by ultrasonification and black yeast fermentation - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a Paeonia lactiflora flower extract as an active ingredient. More specifically, the present invention relates to a cosmetic composition for skin protection with improved skin moisturizing effects, skin barrier improvement effects and antioxidant ability through ultrasonic extraction of Paeonia lactiflora flower and fermentation using black yeast.

Description

COSMETIC COMPOSITION FOR PROTECTING OF SKIN COMPRISING EXTRACT OF PAEONIA LACTIFLORA BY ULTRASONIFICATION AND BLACK YEAST FERMENTATION}

The present invention relates to a composition containing an ultrasonic extract of black yeast fermented peony flower having skin moisturizing function and antioxidant activity as an active ingredient, and the cosmetic composition may be usefully used in cosmetics having skin moisturizing and antioxidant effects.

In 2003, the major causes of death in Korea were cancer, cerebrovascular disease, cardiovascular disease, and diabetes mellitus (Korea National Statistical Office: The cause of death Statistics 2003. Annual Report of on the Cause of Death Statistics.Korea National Statistical Office, 2004 ). Reactive oxygen species (ROS) may be a major factor causing chronic degenerative diseases such as cardiovascular disease, diabetes mellitus, cancer, degenerative neuropathy and aging, and antioxidants are known to help prevent chronic degenerative diseases. (Bray, TM Nutrition. 16, pp 578-581, 2000). Since all aerobic organisms inevitably carry harmful free radical species during metabolism, all organisms must have a mechanism to remove these free radical species in order to survive. Active oxygen species can be broadly classified into two types: compounds that form oxygen radical species, such as hydrogen peroxide, and oxygen radical species. As is already known, hydrogen peroxide is converted to oxygen or water by catalase or peroxidase, but in the case of oxygen radical species removal mechanism, oxygen radical species other than the mechanism for removing O2- (superoxide anion) The mechanism of elimination is not yet clear.

Hydroxy radicals, a type of oxygen radical species, are formed by the reaction of H ₂ O ₂ and O ₂ in living organisms and are known to exist in almost all biomolecules (Fridovich et al., Ann. Rev. Biochem., 44, pp 147-159, 1975; Tauber et al., Blood, 53, pp 666-676, 1979). Therefore, it is obvious that defense against highly reactive hydroxy radicals is inevitably required to maintain homeostasis in vivo. In this respect, organic materials such as ascorbic acid and uric acid are known to remove hydroxy radicals by chemical reaction, but this is not basically removing hydroxy radicals but converting them into less reactive radicals, as well as oxidizing agents in vivo. It has been reported to react with transition metals, such as iron, to promote the production of reactive oxygen radical species (Borg, DC and Schaich, KM, Handbook of Free Radicals and Antioxidant in Biomedicine, Vol. I, pp 63-). 80, CRC press, 1989). Physiological processes radicals generated during ^{_{(O 2 -, H 2 O}} 2, OH and O ₂₎ is a free radical reaction in which a very strong reactivity caused by these represents the destruction effect of major macromolecules, including lipids (Davies , KJA and Goldberg, AL, J. Biol. Chem., 262, p8220-8226, 1987). Their production is increased by a number of factors that lead to normal metabolic processes, photosensitization reactions, drug metabolism processes and other cellular metabolism abnormalities, so that living organisms are always exposed to the deleterious effects of free radical reactions caused by them. (Morehouse, LA and Thomas, CE, J. Free Radicals Biol. Med., 1, p8, 1985).

In particular, if cells continue to accumulate harmful effects due to their harmful radicals as they age, they may cause aging of the cells as well as diseases related to various adult diseases such as carcinogenesis, arteriosclerosis, heart disease and skin aging. It has been suggested as a cause of human disease occurrence and aging (Harman, D., Free Radicals in Biology, Academic Press, New York, pp 255-275, 1982). Therefore, the study aims to find candidates that are expected to be useful in the treatment of diseases such as lipid metabolism, immune diseases and cancer by inhibiting free radical reaction in the body by reactive oxygen metabolites or removing harmful free radicals accumulated in the human body. Have been going on.

Meanwhile, Korean Laid-Open Patent Publication No. 2013-0049040 discloses a cosmetic composition containing a taxoxyline glycoside as an active ingredient and a method of extracting a peony extract, wherein the peony extract has excellent skin whitening effect, wrinkle improvement effect, anti-inflammatory effect, and the like. Doing.

The present inventors have studied the possibility of applying to cosmetics in various natural products, and as a result, the skin moisturizing effect, skin barrier improvement effect and antioxidant effect in black yeast fermented peony flower ultrasonic extract prepared by fermenting the ultrasonic extract of peony flower with black yeast mycelium After confirming, the present invention has been completed.

In addition, the present inventors use ultrasonic extraction instead of the conventionally used methods such as hot water extraction, room temperature extraction, warm extraction and the like, when the ultrasonic extract fermented with black yeast to produce black yeast fermentation peony flower ultrasonic extract, moisturizing and The present invention was completed by discovering that the antioxidant effect is greatly improved.

Therefore, an object of the present invention is to provide a cosmetic composition comprising the black yeast fermented peony flower ultrasonic extract as an active ingredient. In addition, the present invention confirmed the skin moisturizing effect (Experimental Example 1), skin barrier improvement effect (Experimental Example 2), antioxidant effect (Experimental Example 3) of the black yeast fermented peony flower ultrasonic extract and a cosmetic composition comprising the same.

The above object is achieved by a cosmetic composition for improving skin barrier function, which contains black yeast fermented peony flower ultrasonic extract obtained by fermenting the peony flower ultrasonic extract with black yeast.

In addition, an object of the present invention is achieved by the cosmetic composition for antioxidant containing black yeast fermented peony flower ultrasonic extract obtained by fermenting the peony flower ultrasonic extract with black yeast.

Preferably, the black yeast fermented peony flower ultrasonic extract may be contained in 0.0001 ~ 30.0% by weight relative to the total weight of the cosmetic composition.

Also preferably, the peony ultrasonic extract is purified water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol, dichloromethane, chloroform After putting in an extraction solvent selected from the group consisting of ethyl ether, butylene glycol, hexane and mixtures thereof, it can be extracted with an ultrasonic extractor for about 1 to 12 hours.

Also preferably, the black yeast fermented peony flower ultrasonic extract may be obtained by fermenting the peony flower ultrasonic extract for 1 to 7 days while maintaining pH 5 to 7 under a temperature condition of 20 to 40 ℃ as black yeast. .

Also preferably, the cosmetic composition is composed of a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing, an oil, a powder foundation, an emulsion foundation, a wax foundation and a spray It can have any formulation selected from the group.

The present invention can be applied to skin cosmetics and harmless to the human body can provide a cosmetic composition containing as an active ingredient black yeast fermented peony flower ultrasonic extract which is a very safe natural extract.

Black yeast fermented peony flower ultrasonic extract according to the present invention shows a moisturizing effect (Experimental Example 1), skin barrier improvement effect (Experimental Example 2), antioxidant capacity (Experimental Example 3). In addition, the cosmetic composition containing the black yeast fermented peony flower ultrasonic extract of the present invention has excellent skin moisturizing and antioxidant effects compared to the conventional peony flower extract-containing cosmetic composition.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and conform to the meanings as commonly understood by one of ordinary skill in the related art of the present invention. Also described herein are preferred methods or samples, but similar or equivalent ones are within the scope of the present invention.

The term "about" means 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, by reference quantity, level, value, number, frequency, percentage, dimension, size, amount, weight, or length. By amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length, varying by 4, 3, 2 or 1%.

In the present invention, the terms "comprises" and "comprising", unless otherwise necessary in the context, include a given step or component, or step or group of components, but any other step or component, or step Or it should be understood that the group of components is not excluded.

The present invention provides a skin moisturizing and antioxidant composition containing black yeast fermented peony flower ultrasonic extract as an active ingredient.

Peeonia lactiflora ) grows in the mountainous region, several stems stand out from a single plant, about 60 cm high. There are no hairs on the leaves and stems, and several roots come out, but they are thin and long, pointed cylinders are thick. The leaves are shifted and the lower one is a double leaf with three small leaves twice. Small leaf is barso or oval, but sometimes divided into 2 ~ 3 leaves. Leaf veins and petioles are red.

Flowers bloom in May-June one at the end of the stem, large and beautiful, grown 10cm in diameter. Flower colors vary from red to white, and there are many horticultural varieties. Calyxes are 5, green, with flat edges, attached to the ends, and the outermost one is leaf-shaped.

The black yeast fermented peony flower ultrasonic extract of the present invention may be obtained by preparing a peony flower ultrasonic extract and fermenting it with black yeast. Preferably, first, the peony is pulverized, and then water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl It was placed in an extraction solvent selected from the group consisting of ether, butylene glycol, hexane and mixtures thereof, followed by ultrasonic extraction for about 1 to 12 hours while maintaining pH 5 to 7 at a temperature condition of 20 to 40 ° C (room temperature). By extracting the peony flower ultrasonic extract can be prepared. The time of the ultrasonic extraction may vary depending on the amount of the sample to be extracted, and may undergo a process of filtering or purifying the extracted result.

Among the solvents, alcohol is preferable, and ethanol is more preferable. At this time, the ethanol is preferably 70% ethanol. A suitable amount of the extraction solvent is 3 to 15 times the dry weight of peony flowers, more preferably 5 to 10 times.

In addition, the peony flower ultrasonic extract of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

The peony flower ultrasonic extract black yeast ( Aureobasidium pullulans ) to produce black yeast fermented peony flower ultrasonic extract.

Black yeast ( Aureobasidium pullulans ) are microorganisms used to make beer and wine. They are a group of single-celled organisms that are fungi or fungi but do not have mycelia and do not have photosynthesis or motility. It is a microorganism that humans have used in food since 5,000 years ago, and yeast itself is used as a cheap fat or protein source. It contains abundant vitamin B group, and also contains vitamin D. In addition, the black yeast is a microorganism that produces beta-glucan (β-Glucan) is said to have a moisturizing effect, antioxidant, and sedative effect even with the black yeast ferment itself. Black yeast beta-glucan (β-glucan) contains a large amount of ingredients known to have physiological activities such as anti-cancer, inhibiting insulin secretion.

And the black yeast fermented peony flower ultrasonic extract of the present invention means that obtained by fermenting the peony flower ultrasonic extract with the black yeast ( Aureobasidium pullulans ) for 1 to 7 days. Preferably, 1 to 5% by weight of glucose may be added to the ultrasonic extract of peony flower as a carbon source, and 0.1 to 1% of peptone, preferably 0.2 to 0.5% by weight, may be further sterilized. Black yeast ( Aureobasidium so that 20 ~ 30g / L here pullulans ) strain culture can be inoculated. The culture can be incubated at 5 to 7 at 20 to 40 ° C. for 1 to 7 days using a 5 L fermenter. After the culture, it is preferable to sterilize the culture solution by centrifugation so that the culture is not further cultured after primary removal.

According to a preferred embodiment of the present invention, the content of the ultrasonic extract of black yeast fermented peony flower added to the cosmetic composition is 0.0001 to 30.0% by weight, preferably 0.001 to 25.0% by weight, most preferably based on the total weight of the cosmetic composition. Is 0.1 to 10.0% by weight. When the content of the ultrasonic extract of black yeast fermentation peony flower is less than 0.0001% by weight, there is no antioxidant, moisturizing, and skin barrier improvement effects. There are problems with safety and stability and it is not economical.

The cosmetic composition of the present invention includes a cosmetic composition comprising a cosmetically acceptable base.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto.

More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or sorbitan.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystalline as carrier components Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The free free radical scavenging ability of the black yeast fermented peony flower ultrasonic extract of the present invention showed better antioxidant activity compared to L-ascorbic acid, which is a standard substance. In addition, the peony flower is a medicinal herb that has been used for a long time or edible extracts of the present invention extracted therefrom also have no problems such as toxicity and side effects.

Hereinafter, the present invention will be described in more detail with reference to production examples. These preparation examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these preparation examples in accordance with the gist of the present invention to those skilled in the art. Will be self-evident.

Production Example  Peony Flower Purified water  Preparation of Extract

After the peony flower was lyophilized and ground, it was extracted using a magnetic stirrer for 12 hours by adding purified water corresponding to 10 times the weight of the peony flower sample. After filtering under reduced pressure to separate the extract and the residue, purified water corresponding to 5 times the weight of the peony flower at the start of extraction was added to the obtained residue and extracted using a magnetic stirrer for 6 hours. After filtration under reduced pressure again, purified water corresponding to 1 times the weight of the peony flower at the start of extraction was added to the obtained residue, and the mixture was extracted under reflux three times for 5 hours, cooled, and filtered through a Whatman # 3 filter paper. Concentrated under reduced pressure and freeze-dried below 50 ℃. The lyophilisate was dissolved to 2% (W / V) using 50% glycerin to prepare a peony flower purified water extract.

Production Example  2. Preparation of Peony Flower Extract

A peony flower ethanol extract was prepared in the same manner as in Preparation Example 1, except that the extraction solvent was replaced with 70% (v / v) ethanol in Example 1.

Production Example  3. Preparation of Peony Ultrasonic Extract

Peony flowers were selected and thoroughly washed, and then ground to finely pass through a 300 mesh sieve. After extracting 70% (v / v) ethanol to 100g / L ethanol to extract the peony flower extract at room temperature for 4 hours by using an ultrasonic extractor (natural solution), and then cooled, and then filtered with whatman # 3 filter paper , Concentrated under reduced pressure and freeze-dried at below 50 ℃. The lyophilisate was dissolved to 2% (W / V) with 50% glycerin to obtain a peony ultrasonic extract.

Production Example  4. Black yeast  Preparation of Fermented Peony Flower Ultrasonic Extract

To the peony flower ultrasonic extract prepared in Preparation Example 3 can be sterilized by adding 2% (W / V) of glucose as a carbon source and further adding 0.5% (W / V) of peptone. Aureobasidium black yeast so that 25g / L pullulans ) strain culture can be inoculated. Cultivation can be carried out using a 5L fermenter for 7 days, maintained at pH 6 at 37 ℃. After the culture, the culture solution was centrifuged and sterilized (121 ° C., 15 minutes, 1.5 atm) so that the culture was no longer cultured after the first removal.

After fermentation, the peony flower fermentation product from which the culture bacteria were first removed may be refluxed by adding ethanol to form a final 70% (v / v) ethanol aqueous solution, filtered, and filtered. When the filtration is completed, the extract obtained in the extraction step is transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 ° C. or less to prepare the black yeast fermented peony flower ultrasonic extract of the present invention.

Formulation example  1.Containing Peony Flower Extract Cosmetics  Preparation of the composition

Cosmetics (Formulation Examples 1, 2, 3, and 4) containing the peony flower extracts of Preparation Example 1, Preparation Example 2, Preparation Example 3, and Preparation Example 4, respectively, were prepared. As a cosmetic composition (Comparative Formulation Example 1) containing glycerin and 1,3-butylene glycol, and a cosmetic composition (Comparative Formulation Example 2) containing neither an extract nor a moisturizing agent are shown in Table 1 below.

(Unit: Content%) ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Comparative Formulation Example 1 Comparative Formulation Example 2 Peony Flower Purified Water Extract
(Manufacture example 1) 5.0 - - - - - Peony Flower Solvent Extract
(Manufacture example 2) - 5.0 - - - - Peony Ultrasonic Extract
(Manufacture example 3) - - 5.0 - - - Black yeast fermented peony flower ultrasonic extract (manufacturing example 4) - - - 5.0 - - glycerin - - - - 5.0 - 1.3-butylene glycol - - - - 5.0 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystallin 0.5 0.5 0.5 0.5 0.5 0.5 Squalane 5.0 5.0 5.0 5.0 5.0 5.0 Liquid paraffin 2.0 2.0 2.0 2.0 2.0 2.0 Trioctanoine 3.0 3.0 3.0 3.0 3.0 3.0 Polysorbate 1.0 1.0 1.0 1.0 1.0 1.0 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl Acetate 0.1 0.1 0.1 0.1 0.1 0.1 Cyclomethicone 4.0 4.0 4.0 4.0 4.0 4.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative Quantity Quantity Quantity Quantity Quantity Quantity Purified water to 100 to 100 to 100 to 100 to 100 to 100

Experimental Example  1. Moisturizing Effect of Peony Flower Extract

The clinical moisturizing effect and sustainability of the cosmetic compositions of Formulation Examples 1 to 4 and Comparative Formulation Examples 1 and 2 were measured as follows. Through a survey, a group of 150 healthy adult men and women who feel dry skin was randomly formed into 25 groups, and two formulations of Formulation Examples 1 to 4 and Comparative Formulation Examples 1 and 2 were applied twice a day for 4 weeks. One side was applied to both faces. The moisturizing effect was evaluated by quantifying the increase rate (%) of skin electrical conductivity according to the epidermal moisture content using Corneometer CM 820 (Corage + Kazaka, Germany). The experimental results are shown in Table 2 below.

% Increase in skin electrical conductivity division Before use 2 weeks after use 4 weeks after use Formulation Example 1 24.25 40.51 47.24 Formulation Example 2 24.36 41.23 47.53 Formulation Example 3 22.79 53.87 62.54 Formulation Example 4 24.83 59.12 84.82 Comparative Formulation Example 1 25.72 36.18 40.88 Comparative Formulation Example 2 23.12 25.74 26.72

As shown in Table 2, the cosmetics containing the peony flower extract of the present invention (Formulation Examples 1, 2, 3, 4) even when compared to the cosmetics containing the chemical moisturizing glycerin as a comparative formulation (Comparative Formulation Example 1) It was confirmed that the moisturizing effect of the better. In particular, in the case of Formulation Example 4 containing black yeast fermented peony flower extract, the increase rate of skin electrical conductivity was very excellent compared to Formulation Examples 1 to 3 and Comparative Formulation Examples 1 and 2. In general, since the skin conductivity is proportional to the skin moisture content, the above results confirm that the cosmetics containing the black yeast fermented peony flower ultrasonic extract maintain a higher skin moisture content than the cosmetics that do not. Moreover, skin irritation could not be observed in all the subjects who apply this cosmetic to the skin.

Experimental Example  2. Peony Flower Extract Skin barrier  Improvement effect

The subjects were 25 healthy women at 25 ° C, 45% relative humidity and no air flow. Skin barrier damage was caused by using a 1% aqueous solution of sodium lauryl sulfate (SLS), and the cosmetic composition of Formulation Examples 1 to 4 was applied to the upper arm of the arm, followed by TEWAMETER TM 210 (C + K eletronic GmhH. Germany) to measure the transdermal moisture loss. In other words, the TEWL (Transepidermal Water Loss) value was measured by measuring the amount of TEWL change over time to determine the skin barrier recovery rate (%). The experimental results are shown in Table 3 below.

division Skin barrier recovery rate (%) 2D 4D 6D 8D Formulation Example 1 20.03 35.39 56.12 66.35 Formulation Example 2 21.31 33.59 54.76 64.89 Formulation Example 3 25.18 45.46 66.37 78.36 Formulation Example 4 50.19 66.35 82.37 95.01

As a result of measuring the skin barrier improvement effect as shown in Table 3, it was found that Formulation Example 4 containing the black yeast fermented peony flower ultrasonic extract was significantly improved compared to Formulation Examples 1 to 3.

Experimental Example  3. In vitro DPPH  Antioxidant activity measurement by method

DPPH method (Blois, M. S.: Antioxidant determination by the use of a stable free radical, Nature, 26, 1199-1200, 1958) was used to determine the in vitro antioxidant effect of the peony flower extract. DPPH (1,1-diphenyl picrylhydrazyl) method is a biological activity showing the antioxidant activity of tocopherol, ascorbate, flavonoid compounds, aromatic amines, Maillard browning products, peptides, etc. As a standard product, L-ascorbic acid was used as a standard product to measure the antioxidant effect according to the extent of dark purple color fading by reduction by a substance. In each sample, 2 x 10-4 M DPPH was added and absorbance was measured at 517 nm. Was measured. As a result, DPPH free radical scavenging ability of purified water extract of peony flower, 70% ethanol extract, ultrasonic extract and black yeast fermented peony flower extract was 76.5%, 79.9%, 88.8% and 90.2% at 0.05 g / L concentration, respectively. The activity of L-ascorbic acid used as a standard was 82.1%.

Samples and Standards DPPH free radical scavenging ability 0.01 g / L 0.03 g / L 0.05 g / L Peony Flower Purified Water Extract
(Manufacture example 1) 20.6% 46.3% 76.5% Peony Flower Solvent Extract
(Manufacture example 2) 23.2% 50.4% 79.9% Peony Ultrasonic Extract
(Manufacture example 3) 28.8% 60.2% 88.8% Black Yeast Fermented Peony Flower Ultrasonic Extract (Preparation 4) 34.1% 72.6% 90.2% L-ascorbic acid 25.3% 55.0% 82.1%

Peony flower extracts of Preparation Examples 1 to 4 showed high activity comparable to other antioxidants, and especially black yeast fermented Peony flowers extract of Preparation Example 4 showed higher antioxidant activity compared to L-ascorbic acid. It can be seen that. In particular, the black yeast fermented peony flower ultrasonic extract was found to have a significant antioxidant activity in spite of the mixture of natural products, it was found that the antioxidant activity effect is improved through the black yeast fermentation.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Therefore, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (6)

Cosmetic composition for improving skin barrier function containing black yeast fermentation peony flower ultrasonic extract fermented peony flower ultrasonic extract with black yeast. Antioxidant cosmetic composition containing the black yeast fermentation peony flower ultrasonic extract fermented peony flower ultrasonic extract with black yeast. The cosmetic composition according to claim 1 or 2, wherein the black yeast fermented peony flower ultrasonic extract is contained in an amount of 0.0001 to 30.0 wt% based on the total weight of the cosmetic composition. The method according to claim 1 or 2, wherein the ultrasonic extract of the peony flower is purified water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol , Dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, and put in an extraction solvent selected from the group consisting of, and then extracted with an ultrasonic extractor for about 1 to 12 hours, cosmetic composition. According to claim 1 or 2, wherein the black yeast fermented peony flower ultrasonic extract, while maintaining the pH 5 to 7 at a temperature of 20 ℃ to 40 ℃ black peony flower extract, black yeast for 1 to 7 days Cosmetic composition obtained by fermentation. The cosmetic composition according to claim 1 or 2, wherein the cosmetic composition is a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing, an oil, a powder foundation, an emulsion foundation, a wax Having a formulation selected from the group consisting of a foundation and a spray, cosmetic composition.