KR100654063B1 - COSMETIC COMPOSITION FOR PREVENTING SKIN AGING COMPRISING beta;-1,6-BRANCHED-BETA;-1,3-GLUCAN STABILIZED IN NANOLIPOSOME AS ACTIVE INGREDIENT - Google Patents

Abstract

A skin aging preventing composition comprising nanoliposome is provided to stabilize beta-1,6-branched-beta-1,3-glucan derived from Schizophyllum commune in the nanoliposome so as to improve the skin permeability thereof, thereby being usefully used for preventing skin aging. The cosmetic composition for preventing skin aging comprises 0.1-20 wt.% of nanoliposome, where beta-1,6-branched-beta-1,3-glucan derived from Schizophyllum commune is stabilized as an effective ingredient. The nanoliposome is prepared from a mixture consisting of polyol, surfactant, phospholipid, oily ingredient, fatty acid and water, and has an average particle diameter of 100-300 nanometers.

Description

Cosmetic composition for preventing skin aging comprising nanoliposome stabilized by inclusion of beta-1,6-branched-beta-1,3-glucan as an active ingredient {-1,6-branched-β -1,3-glucan Stabilized in Nanoliposome As Active Ingredient}

The present invention relates to a cosmetic composition for preventing skin aging.

Beta-glucans are commonly found in yeast cell walls, mushrooms, and cereals. In 1941, Dr. Louis Pillemer of the United States discovered a substance involved in nonspecific immune responses in yeast cell walls. It comes from what was named. Zymosan was a simple yeast extract that contained a large amount of protein or fat in addition to beta-glucan, which was associated with allergic side effects. Zymosan has been proven to be an immune-activated substance by beta-glucan, named polymer polysaccharides extracted from the cell walls of yeast by Dr. Nicholas Diluzio of the United States in the early 1960s. Glucan, a type of polysaccharide, is a glucose polymer containing only D-glucose as a constituent sugar, and is divided into α or β types depending on the form of linkage. In particular, beta-glucan has side chains at the 4th (β-1,4-bond) or 6th (β-1,6-bond) carbons in the backbone, in which glucose units are linked by β-glucoside bonds at positions 1 and 3 It is known that the structural difference with or without the side chain influences the physicochemical properties.

Many studies have reported that beta-glucan stimulates humoral and cellular immunity in humans and animals to amplify the immune system and act as an immunomodulator ( Carbohydrate Research , 1981, 89: 121-135; Cancer , 1992, 69: 1188-1194; Invasion Metastasis , 1981, 1: 71-84). Many mechanisms for the anti-cancer effects of beta-glucan are known, and these beta-glucans do not directly attack cancer cells, but rather immune to normal immune cells such as macrophage, natural killer cells, and T cells. It is known to activate the function, inhibit the proliferation and recurrence of cancer cells and promote the increase of immune cells.

Beta-glucan has been widely used as a cosmetic material with various effects on the skin in addition to the immune activation and anticancer effects. Skin is constantly damaged by environmental factors such as sun, wind, dust and sand particles, wear, shaving, abrasions, contact with harmful chemicals and occupational exposure. Damaged skin is susceptible to germs, which accelerates the damage. Macrophages activated by beta-glucans eliminate germs and acne bacteria by phagocytosis and prevent secondary infection. In addition, by preventing the peroxidation of squalene, the main component of sebum by ultraviolet rays, it prevents erythema and purulent, reduces inflammation by reducing the sensitivity of keratinocytes to ultraviolet rays, and increases the number of Langerhans cells to increase skin cell immunity. It is known to block the development of dermatitis and skin cancer by sensitization ( J. of Leukocyte Biology , 1987, 42: 95-105).

One of the biggest features of the aging process is the increase in skin wrinkles and decrease in elasticity, in addition to a decrease in overall immune defense function. Beta-glucan, administered to aging or wrinkled skin, activates macrophages to produce endotherial cell growth factor (ECGF). ECGF promotes collagen biosynthesis to remove wrinkles and increase skin elasticity. Keeps the skin

In general, beta-glucan is known to be one of the very rare substances that can pass through the epidermal layer and can pass through the dermal layer despite its large molecular weight. However, since the molecular weight of beta-glucan is usually hundreds of thousands to millions of times, the skin penetration rate is considerably slower than other low molecular weight materials, and thus the skin action effect is very low.

Throughout this specification, a number of articles are referenced and their citations are indicated in parentheses. The disclosure of the cited article is incorporated herein by reference in its entirety, so that the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

Thus, the present inventors conducted a study to find a method for promoting the skin permeation of beta-1,6-branch-beta-1,3-glucan derived from the skirt mushroom for the effective skin action of beta-glucan, the beta The present invention was completed by confirming that -1,6-branch-beta-1,3-glucan was included in the nanoliposomes to stabilize the skin penetration effect of the above-mentioned ingredients, thereby improving the skin aging effect.

Accordingly, an object of the present invention is to provide a cosmetic composition for preventing skin aging containing nanoliposomes stabilized by inclusion of beta-1,6-branch-beta-1,3-glucan as an active ingredient.

Other objects and advantages of the present invention will become apparent from the following detailed description and claims.

According to an aspect of the present invention, the present invention is a nanoliposome stabilized by inclusion of beta-1,6-branched-beta-1,3-glucan (β-1,6-branched-β-1,3-glucan) It provides a cosmetic composition for preventing skin aging containing as an active ingredient.

As used herein, the term 'anti-aging of skin' refers to all of the skin moisturizing, skin elasticity enhancement, skin thickness increase, skin wrinkle improvement, skin irritation relief and skin damage recovery action.

In the cosmetic composition of the present invention, the active ingredient exhibiting anti-aging effect is preferably beta-1,6-branched-beta-1,3-glucan (β-1,6-branched-β-1,3-glucan), which is preferable. Preferably, it is beta-1,6-branch-beta-1,3-glucan, which is a high purity beta glucan produced in the culture of the mushroom mushroom mycelium. Schizophyllum commune is a woody fungus belonging to the genus Cortex fungus of the Basidiomycetes according to The Agaricales in modern taxonomy (1975), beta-1,6-branch. -Beta-1,3-glucan polysaccharide is a strain that produces extracellularly. Skirt mushrooms can be harvested from the wild and identified by the following morphological characteristics or taxonomic indicators. The fruiting body of the skirting mushroom has no stalk, the side of the shade is attached to the substrate, usually 1.0-3.0 cm, the shape is fan-shaped or clam-shaped, folds with bells, the ends are irregularly divided, and fine hairs are covered. Wrinkles are white but pale gray or pale brown when mature, and tissues contract when dry, but recover when water is absorbed. The spores are white and the spores are 4-6 × 1.5-2 μm in size and are smooth and white. Skirt mushrooms are caused by bunches of hardwoods and stumps, and the detailed treatment and shape of the mushrooms are Korean mushrooms (Kim Sam-soon, co-authored by Kim Yang-seop, Yu Poong Publisher, 1990) and Singer's The Agaricales in modern taxonomy, 1975).

Meanwhile, beta-glucan uses beta-type glucose bonds such as beta-1,3-bonds, beta-1,4-bonds, and beta-1,6-bonds without distinction. In addition, as can be seen in the following mushrooms, depending on the origin, there are many differences such as uniformity, branching degree, molecular weight, and tertiary structure of sugars, so that the physical and chemical properties and biological functions are often completely different.

For example, beta glucan of Ganoderma licidum is mainly extracted from the cell wall, but is secreted in small amounts in addition to cells, and has a binding form of mannose, galactose, etc. in addition to glucose. Beta glucan of cloud mushroom (C oriolus versicolor ) is extracted from cell wall and contains many beta-1,4-binding types in addition to beta-1,3-binding. Beta glucans of shiitake mushrooms ( Lentinus edodes ) are extracted from the cell wall and have beta-1,5-branch-beta-1, which have two beta-1,5- residues within every five residues of the beta-1,3 main sugar chain, 3-glucan. Beta glucan of Pleurotus ostreatus is extracted from the cell wall, consists of several types of glucan, and has a binding form of mannose, galactose, etc. in addition to glucose. In addition, the beta-glucans of yeast ( Saccharomyces cerevisiae ) are extracted from the cell wall and have little branching, heterogeneous and insoluble.

On the other hand, the beta-glucan of the Schizophyllum commune of the present invention is a uniform beta-1,6-branch-beta-1,3-glucan, beta- per every three residues of the beta-1,3 main sugar chain. By having 1,6 residues, the beta-glucans of the above-mentioned mushrooms have a heterogeneous sugar composition and structure, but they have a branched uniform and unique structure, stable neutral polysaccharides secreted out of the cell, and consist only of glucose. The molecular weight (MW) is 2-5 million Da, which is considerably larger than other mushrooms in the range of several hundred thousand to 2 million Da. Therefore, the beta-glucan of the squirrel mushroom has different physical properties and functionality from other beta glucans, but has a considerable difficulty in skin permeation due to its large molecular weight.

It is apparent to those skilled in the art that beta-1,6-branch-beta-1,3-glucan included in the cosmetic composition of the present invention may be separated from a skirt mushroom, but chemically synthesized may have the same effect.

According to a preferred embodiment of the present invention, the beta-1,6-branch-beta-1,3-glucan is 0.001-40.0% by weight, more preferably 0.1-25.0% by weight relative to the total weight of nanoliposomes. In addition, the content of the nano liposome is 0.1-20.0% by weight, preferably 1.0-10.0% by weight relative to the total weight of the cosmetic composition. If the content of the nanoliposome is less than 0.1% by weight can not expect a clear skin aging effect, if the content exceeds 20.0% by weight does not appear to increase the apparent effect of increasing the content.

As used herein, the term "nanoliposomes" refers to liposomes having an average particle diameter of 10-500 nm as having the form of conventional liposomes. According to a preferred embodiment of the invention, the average particle diameter of the nanoliposomes is 100-300 nm. When the average particle diameter of the nanoliposomes exceeds 300 nm, the improvement of skin penetration is very weak among the technical effects to be achieved in the present invention, and the formulation is unstable.

Nanoliposomes of the present invention are prepared by a mixture comprising (i) polyols, (ii) surfactants, (iii) phospholipids, (iii) oily components, (v) fatty acids and (iii) water, Eg ethanol).

The polyol used in the nanoliposome of the present invention is not particularly limited, and preferably, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isoprene glycol, pentylene glycol, erythritol, xyitol and Selected from the group consisting of sorbitol, most preferably propylene glycol. The amount used is 10.0-80.0% by weight, preferably 30.0-70.0% by weight, based on the total weight of nanoliposomes.

Surfactants used in the preparation of the nanoliposomes of the present invention may be any known in the art, for example, anionic surfactants, cationic surfactants, amphoteric surfactants or nonionic surfactants may be used. , Preferably anionic surfactants and nonionic surfactants are used. Specific examples of anionic surfactants include alkylacylglutamate, alkylphosphates, alkyllactylates, dialkylphosphates, alkyllactates and trialkylphosphates. Specific examples of nonionic surfactants include alkoxylated alkylethers, alkoxylated alkylesters, alkylpolyglycosides, polyglyceryl esters and sugar esters. More preferably, it is a mixture of the alkyl lactate which is anionic surfactant, and the polysorbate which is a nonionic surfactant. Most preferably, the alkyl lactate is sodium stearoyl lactate. The amount of the surfactant to be used is generally 0.1-10.0% by weight, preferably 0.3-5.0% by weight based on the total weight of the nanoliposomes.

Phospholipids, another component used in the preparation of the nanoliposomes of the present invention, are used as amphoteric lipids, including natural phospholipids (eg, egg yolk lecithin or soy lecithin, sphingomyelin) and synthetic phospholipids (eg dipalmitoyl). Phosphatidylcholine or hydrogenated lecithin), preferably lecithin. Most preferably, the lecithin is hydrogenated lecithin. In the preparation of the nanoliposomes of the present invention, the amount of lecithin used is 0.5-20.0% by weight, and preferably 2.0-8.0% by weight, based on the total weight of the nanoliposomes.

The oil component used in the preparation of the nanoliposomes of the present invention may be various oils known in the art, for example, hydrocarbon-based oils such as hexadecane and paraffin oil; Synthetic synthetic oils such as isopropyl myristate; Silicone oils such as dimethicone and cyclomethicone series; Animal and vegetable oils such as sunflower oil, corn oil, soybean oil, avocado oil, sesame oil, jojoba oil, nut oil, squalane and fish oil; Ethoxylated alkyl ether oils; Propoxylated alkyl ether oils; Sphingoidoid lipids such as phytosphingosine, sphingosine and sphinginine; Cerebroside; cholesterol; Cytosterol; Cholesteryl sulfate; Cytosteryl sulfate; C _10-40 fatty alcohol or ceramide. Preferably, hydrocarbon oils such as hexadecane and paraffin oil, silicone oils such as dimethicone and cyclomethicone, or sunflower oil, corn oil, soybean oil, avocado oil, sesame oil, jojoba oil, nut oil, squalane and It is a plant and animal oil such as fish oil. Preferred among hydrocarbon oils are paraffin oils, preferred isopropyl myristate among ester oils, dimethicone is preferred among silicone oils, and nut oils and squalanes are preferred among animal and vegetable oils. More preferably, it is paraffin oil, isopropyl myristate, dimethicone, squalane or nut oil, most preferably a mixture of the five oils. The amount used is 2.0-30.0% by weight based on the total weight of nanoliposomes, preferably 3.0-20.0% by weight.

The fatty acids used in the preparation of the nanoliposomes of the present invention are higher fatty acids, preferably saturated or unsaturated fatty acids of C _12-22 alkyl chains such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid. And most preferably stearic acid. The content of fatty acid is 0.05-3.0% by weight, preferably 0.1-1.0% by weight relative to the total weight of nanoliposomes.

Water used in the preparation of the nanoliposomes of the present invention is generally deionized distilled water, the amount of which is used is 3.0-20.0% by weight based on the total weight of the nanoliposomes.

The preparation of nanoliposomes can be accomplished through various methods known in the art, but most preferably, a mixture comprising the above components is applied to a high pressure homogenizer. The preparation of nanoliposomes by high pressure homogenizer can be carried out under various conditions (eg pressure, frequency, etc.) depending on the desired particle size, preferably 1-5 times high pressure homogenizer under 600-1200 bar pressure. Nanoliposomes are prepared by passing through.

The nanoliposome system thus prepared has the advantage of maximizing the skin penetration effect by dissolving various types of poorly soluble substances and stabilizing unstable substances.

Ingredients included in the cosmetic composition of the present invention includes components commonly used in cosmetic compositions in addition to nanoliposomes containing beta-1,6-branch-beta-1,3-glucan derived from S. mushroom as an active ingredient, Such as conventional auxiliaries such as stabilizers, solubilizers, vitamins, pigments and flavorings, and carriers.

The cosmetic composition of the present invention having an anti-aging effect may be prepared in any formulation commonly prepared in the art, for example, a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap. , Surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays, and the like, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention promoted skin permeation of the active ingredient at all times by stabilizing the beta-1,6-branch-beta-1,3-glucan derived from the squirrel mushroom in the nanoliposomes, thereby beta-derived beta -1,6-Branch-beta-1,3-glucan increases skin moisturization, improves skin elasticity, increases skin thickness, improves skin wrinkles, reduces skin irritation and repairs skin damage. Is improved. This fact is specified in the following examples.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Preparation Example 1 Preparation of Beta-1,6-Branch-Beta-1,3-Glucan

The mycelium of squirrel mushrooms was washed several times with distilled water from spores obtained from wild squirrel of wild squirrel mushrooms, and then yeast-malt extract agar medium (3 g of yeast extract, 3 g of malt extract, 5 g of peptone, 10 g of glucose, 15 g of agar and 1 L of distilled water) were plated and cultured at 25 ° C for one week. On the other hand, the mycelia were incubated in a test tube containing a yeast-malt extract agar medium and stored at 4 ℃, used subcultured every month. After aseptic homogenization of the mycelia grown by slope culture, it was inoculated in yeast-malt extract liquid medium and incubated at 25 ° C. and 200 rpm for about 5 days. At this time, the concentration of the measured mycelium was about 5.0 g / l, and the concentration of beta-1,6-branch-beta-1,3-glucan was about 10.0 g / l. The solution was passed through a filtration membrane having micropores of 1 μm and 0.45 μm in order to completely remove the mycelia, and then slowly added 3-4 times the amount of ethyl alcohol to beta-1,6-branch-beta-1 of the polymer. , 3-glucan was collected by collecting. The recovered beta-glucan is again dissolved in the initial solution and the same amount of water, gradually reducing the amount of ethyl alcohol used to a less than one-fold amount, by adding 1-4 times more ethyl alcohol in the same way to purify and homogeneous. One beta-1,6-branch-beta-1,3-glucan was recovered. The recovered beta-1,6-branch-beta-1,3-glucan was hot-air dried at a temperature of 20-100 ° C to volatilize the alcohol components, and then frozen at -70 ° C and dried using a lyophilizer. The dried beta-1,6-branch-beta-1,3-glucan was triturated with a grinder to obtain a powdered compound. In order to check the components of the powder compound prepared above, the spectrum was obtained by infrared absorber (Biorad, FTS-40). It was confirmed that it is 1,3-glucan.

Preparation Example 2 Preparation of Stabilized Nanoliposomes by Inclusion of Beta-1,6-Branch-Beta-1,3-Glucan

To prepare a nanoliposome stabilized by inclusion of beta-1,6-branch-beta-1,3-glucan prepared in Preparation Example 1, B phase was added to A phase in the composition of Table 1 below, and uniform After wet, the phase C was added and mixed by heating to 70-80 ° C. The solution was passed through a high pressure homogenizer (Microfluidizer M-110F, Microfluidics International Corporation) 1-5 times under conditions of 600-1200 bar and cooled to beta-1,6-branch-beta-1, Nanoliposomes having a particle size of 100-300 nm in diameter to which 3-glucan was stabilized were obtained.

Prize ingredient Content (% by weight) A glycerin to 100 B Hydrogenated lecithin 5.0 Floating paraffin 1.0 Macadamia Nut Oil 1.0 Squalane 1.0 Dimethicone 1.0 Isopropyl myristate 1.0 Sodium Stearoyl Lactate 0.5 Polysorbate-80 0.5 Stearic acid 0.5 C Beta-glucan derived from Schisandra mushroom 20.0 Potassium hydroxide Quantity Purified water 5.0

Preparation Example 3: Preparation of a composition comprising nanoliposomes stabilized beta-1,6-branch-beta-1,3-glucan

A composition comprising nanoliposomes stabilized beta-1,6-branch-beta-1,3-glucan derived from a skirt mushroom of the present invention (Example 1) and beta derived from a skirt mushroom without stabilization with the nanoliposomes A composition containing -1,6-branch-beta-1,3-glucan itself (Comparative Example 1) and a composition not containing all of the above components (Comparative Example 2) were prepared in the compositions shown in Table 2 below.

ingredient Example 1 (% by weight) Comparative Example 1 (% by weight) Comparative Example 2 (% by weight) Nanoliposomes (Manufacturing Example 2) 10.0 - - Beta-glucan (Preparation Example 1) - 2.0 - A Cetostearyl alcohol 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 Microcrystallin 0.7 0.7 0.7 Squalane 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 Trioctanoine 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 Tocopherol Acetate 0.2 0.2 0.2 Pyromethicone 3.0 3.0 3.0 BHT 0.05 0.05 0.05 B glycerin 4.0 4.0 4.0 1,3-butylene glycol 2.0 2.0 2.0 EDTA-2Na 0.05 0.05 0.05 Purified water To 100 To 100 To 100 Incense, preservative Quantity Quantity Quantity

Experimental Example 1: Measurement of the stability of the formulation

The stability test of the formulation for the cosmetic containing the nanoliposome stabilized beta-1,6-branch-beta-1,3-glucan derived from the skirt mushroom of the present invention was carried out by the following method.

The composition of Example 1 and Comparative Examples 1 and 2 prepared in Preparation Example 3 was stored in an opaque glass container in a thermostat maintained at room temperature and 45 ° C. for 12 weeks, and was kept constant at 4 ° C. After immersing in an opaque glass container in a completely shaded refrigerator for 12 weeks, the color change / odor, separation degree was measured. At this time, the degree of product discoloration / deodorization / separation was evaluated by classifying the following six grades, and the results are summarized in Table 3 below.

<Production degree of crystallization>

0: no change. 1: very little discoloration / odor / separation.

2: a little discoloration / odor / separation. 3: Slightly discolored / deodorized / separated.

4: Severe discoloration / odor / separation. 5: Extremely severe discoloration / odor / separation.

Temperature Discoloration / deodorization / separation degree Example 1 Comparative Example 1 Comparative Example 2 45 ℃ 0 0 0 Room temperature 0 0 0 4 ℃ 0 0 0

As can be seen in Table 3, the compositions of Example 1 and Comparative Examples 1 and 2 were all stable without discoloration / odor / separation under all temperature conditions.

Experimental Example 2: Skin Permeability Measurement

In order to determine the skin permeability of beta-1,6-branch-beta-1,3-glucan derived from the skirting mushroom, Franz permeation cells were measured on the skin of hairless mice. Before the test, the abdominal skin of male and female 5 to 8 week old rats was taken and cut into an area of 1 cm 2, which was placed in a permeation cell having a diameter of 0.64 cm 2 and clamped. 0.5 g of the composition of Example 1 and Comparative Example 1 was added to one side of the skin (donor container). The opposite side (receptor container) was allowed to come in contact with phosphate buffer and the temperature at the test was maintained at 32 ° C., the actual skin temperature. After the start of the test, a portion of the solvent was taken at regular intervals, and then the amount of beta-1,6-branch-beta-1,3-glucan derived from the skirt mushroom penetrated through the skin was measured using HPLC. Skin permeation (μg / ㎠) per concentration is shown, and the results are shown in Table 4 below.

division Skin penetration per hrs (㎍ / ㎠) 0 4 8 12 24 Example 1 0 1.41 2.85 5.86 12.42 Comparative Example 1 0 0.56 1.14 2.36 4.99

Through the results in Table 4, in the case of the composition comprising a nanoliposome stabilized by inclusion of beta-1,6-branch-beta-1,3-glucan derived from a skirt mushroom (Example 1), the nanoliposomes Compared to the composition containing beta-1,6-branch-beta-1,3-glucan itself (Comparative Example 1) without stabilization, it was found that the skin permeability was very good. . The results indicate that beta-1,6-branch-beta-1,3-glucan derived from skirt mushroom beta-1,6-branch-beta-1 in the case of a composition comprising nanoliposomes stabilized by inclusion It is shown that the skin permeability of, 3-glucan can be improved with high yield.

Experimental Example 3: Moisturizing Effect

In order to evaluate the moisturizing power of each of the cosmetic compositions of Example 1 and Comparative Examples 1 and 2 prepared in Preparation Example 3, the moisture retention capacity and the amount of water evaporation were measured as follows on the skin to which each cosmetic composition was applied. .

Experimental Example 3-1: Measurement of Water Retention Capacity

In the constant temperature and humidity room of 22 ° C. and 45% RH, each of the cosmetic compositions of Example 1 and Comparative Examples 1 and 2 was coated with a predetermined amount (0.03 g / 16 cm 2) inside the forearm of 20 test subjects, and before application The moisture content of the skin after 1 hour and after 2 hours of application was measured, and normal skin without any treatment was used as a control. As a measuring device, a moisture content meter (corneometer CM820, Conrage + Khazaka, Germany) was used to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 5 below.

division Example 1 Comparative Example 1 Comparative Example 2 Control Skin electrical conductivity before application 50 50 50 50 Skin electrical conductivity 1 hour after application 78 65 56 50 Skin electrical conductivity 2 hours after application 67 58 53 50

Experimental Example  3-2: Measurement of Water Evaporation Amount

After tape stripping on the forearm of the arm to weaken the skin's protective film, apply the cosmetic composition to the subject in the same manner as in Experiment 3-1, and then use a moisture evaporator (TEWA meter, koln). , Germany Federal Bureau) was measured by evaporation of water 5 times at 1 minute intervals every 4 hours to obtain an average value. The results are shown in Table 6 below.

division Example 1 Comparative Example 1 Comparative Example 2 Control % Decrease in water evaporation after 4 hours 42.7 21.6 15.6 10.4 % Decrease in water evaporation after 8 hours 78.9 45.3 32.6 20.6 % Decrease in water evaporation after 12 hours 92.1 78.3 51.4 35.6

As shown in the results of Experimental Examples 3-1 and 3-2, the cosmetic composition of Example 1 of the present invention had a higher electrical conductivity of the skin and a higher water loss reduction rate than the cosmetic compositions of Comparative Examples 1 and 2. Therefore, the cosmetic composition of the present invention was found to exhibit an excellent moisturizing effect.

Experimental Example 4: Skin Elasticity Enhancing Effect

The elasticity enhancing effect of the cosmetic composition of the present invention was evaluated through an actual use test. Cosmetic preparations containing nanoliposomes stabilized by inclusion of beta-1,6-branch-beta-1,3-glucan derived from the skirt mushroom of Preparation Example 2 (Example 1) and beta- derived from the skirt mushroom of Preparation Example 1 Cosmetics containing only 1,6-branch-beta-1,3-glucan (comparative example 1) and compositions containing no both (comparative example 2) were used. A group of 30 30-40 year old women were randomly divided into three groups and the cosmetics were applied continuously (twice a day and 6 weeks) around the eyes of the face, and then the degree of elasticity increase and decrease at each site was measured by a cutometer. , C + K, Germany). In addition, each subject was given a questionnaire to personally assess the degree of skin elasticity.

Elasticity-enhancing effect (device evaluation) division Elasticity change (%, n = 10) Example 1 38.5 Comparative Example 1 22.1 Comparative Example 2 5.9

Elasticity enhancing effect (question assessment) division Very effective Effective Slightly effective no effect system Example 1 4 4 2 0 10 Comparative Example 1 2 5 2 One 10 Comparative Example 2 0 2 4 4 10

Through the results of Tables 7 and 8, it can be seen that the beta-1,6-branch-beta-1,3-glucan derived from the skirt mushroom exhibits skin elasticity enhancing effect. In particular, the nanoliposomes stabilized with beta-1,6-branch-beta-1,3-glucan derived from Schizophyllum mushroom exhibited better skin elasticity, and the skin of the subjects who applied the cosmetic composition of the present invention to the skin No stimulation could be observed.

Experimental Example  5: increasing skin thickness

The effect of the cosmetic composition containing the nanoliposome containing beta-1,6-branch-beta-1,3-glucan derived from the skirt mushroom of the present invention on skin thickness was measured.

Each of the cosmetic compositions of Example 1 and Comparative Examples 1 and 2 were divided into three parts (2 times / day, 0.2 g / time) in the area of the left upper arm 2 × 2 cm 2 of 30 women over 30 years old. Application was made for 3 months. The skin thickness at each site was measured before starting the application, and the skin thickness was measured every month to observe the change of skin. Skin thickness was measured using 20 MHz radiofrequency ultrasonography (Dermascan C, Cortex technology, Denmark), and the mean value of 30 patients was recorded. The experimental results are shown in Table 9.

Change in skin thickness division Comparison of Skin Thickness Change (mm) 0 days 30 days 60 days 90 days Example 1 0.02 0.05 0.09 0.11 Comparative Example 1 0.01 0.02 0.05 0.08 Comparative Example 2 0.00 0.01 0.01 0.03

In general, as the body ages, the dermis may experience a decrease in skin thickness due to loss of blood vessels, decreased cell count, and reduced collagen production. Thus, in order to keep skin thickness healthy, continuous production of dermal layer components is required. In this study, the effect on the skin dermal layer of a cosmetic formulation containing nanoliposomes incorporating beta-1,6-branch-beta-1,3-glucan derived from a skirt mushroom was observed. As a result, it was found that the cosmetic composition of Example 1 had a higher skin thickness increasing effect than the cosmetic compositions of Comparative Examples 1 and 2.

Experimental Example 6: Skin wrinkle improvement effect on human skin

The anti-wrinkle effect of the cosmetic composition containing the nanoliposome containing beta-1,6-branch-beta-1,3-glucan derived from the skirt mushroom of the present invention was measured in 15 healthy Korean women. For the subjects with facial wrinkles aged 34 to 48 years, the cosmetic composition containing beta-1,6-branch-beta-1,3-glucan derived from the skirt mushroom of Preparation Example 2 (Example 1) and The skin wrinkle improvement effect of the cosmetic composition (Comparative Example 1) containing only beta-1,6-branch-beta-1,3-glucan derived from the skirt mushroom of Preparation Example 1 was evaluated. The cosmetic composition of Example 1 was used for the face left part of the subject, and the cosmetic composition of Comparative Example 1 was used for the right part for 2 months. After measuring the skin condition on both sides of the face before use of the composition, the same site was remeasured two months after use to measure changes in skin wrinkles. Skin wrinkles were measured in a constant temperature and humidity room at 24 ° C and a relative humidity of 40%. The wrinkles at the tail of the eyes were replicated using a skin visometer (Skin Visiometer SV400, Electronics GmbH., Germany). Measured. The amount of change in skin wrinkles was calculated according to the following formula.

Skin wrinkle change (Δ%) = (T _di -T _d0 ) / T _do × 100

T _di ; Measurement site value on day 60

T _do ; Measurement site value on day 0

As a result of calculation according to the above formula, the skin wrinkle of the site using the cosmetic composition of Comparative Example 1 showed a decrease of 4.9 ± 2.5% (mean ± standard deviation), while the skin of the site using the cosmetic composition of Example 1 Wrinkles showed a decrease of 13.6 ± 3.9%, indicating an excellent skin wrinkle improvement. According to the above results, it can be seen that the cosmetic composition containing nanoliposomes containing beta-1,6-branch-beta-1,3-glucan derived from the skirt mushroom has a great effect on skin wrinkle improvement.

Experimental Example 7: skin irritation alleviation effect-human skin patch test

Example 1 and Comparative Examples 1 and 2 for 20% of the skin irritant lactic acid in 20 women in their 20s and 30s who have never exhibited hypersensitivity to skin irritation and have no skin disease or skin allergy. Human skin patch test was conducted to determine the irritant effect of the cosmetic composition. First the test site was wiped with 70% ethanol and dried. 15 μg of the prepared test substance was added dropwise into a Fin chamber (Finn chamber, 100 × 10, EPITEST, Finland) and then airtightly wrapped inside the forearm of the subject. The patch was applied for 24 hours, the patch was removed, and the test site was marked with a marker pen. After 1 hour and 24 hours, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to check for erythema and edema.

Skin reactions were determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG), and the results are shown in Table 10 below.

-Evaluation criteria and score of skin reaction-

Symbol Score Criteria

  -0 no response

  ± 0.5 faint or light erythema

  + 1 clear but weak erythema, edema and papules

  + + 2 distinct erythema, papules and vesicles

  ＋＋＋ 3 severe erythema and alveolar vesicle

Test substance 24 hours 48 hours Average score (n = 20) ± + + + ± + + + Example 1 5-- --- 2.08 Comparative Example 1 7 1- One - - 4.17 Comparative Example 2 9 3- 1 1- 7.50

As can be seen in Table 10, the cosmetic composition containing the nanoliposome containing beta-1,6-branch-beta-1,3-glucan derived from the skirt mushroom (Example 1) is induced by 5% lactic acid It can be seen that the skin stimulation effect is very excellent.

Experimental Example 8: Efficacy test for healing skin damage by sunlight

The skin color initial value of the irradiated site was measured using a skin colorimeter (Chromameter CM202). After measuring MED (Minimal Erythma Dosage) on the forearm part of the subject using a solar simulator, the erythema was induced by irradiation with 1.5 MED ultraviolet rays, and then the cosmetic compositions of Example 1 and Comparative Examples 1 and 2 were respectively 20 Μl was applied at 4 hour intervals. 12 hours and 24 hours after the ultraviolet irradiation, the skin pigmentation meter was used to measure the erythema of the areas where the compositions were treated, and evaluated the efficacy of burn healing. At this time, the erythema index was converted to the skin pigment measurement value before the first UV irradiation as 100, the results are shown in Table 11 below.

Hours (hr) Erythema index Example 1 Comparative Example 1 Comparative Example 2 0 100.0 100.0 100.0 12 143.2 163.9 185.6 24 121.4 149.6 165.4

As can be seen in Table 11, the erythema index of the cosmetic composition (Example 1) containing the nanoliposome containing beta-1,6-branch-beta-1,3-glucan derived from the skirt mushroom is the lowest It was found that the best sunburn healing effect was shown.

Hereinafter, based on the results of the above experimental example, a cosmetic composition containing the nanoliposomes stabilized beta-1,6-branch-beta-1,3-glucan derived from the skirting mushroom of the present invention is presented. However, the composition of the present invention is not intended to be limited to the following formulation examples. The nanoliposomes disclosed in the following formulation examples are collectively referred to as nanoliposomes stabilized beta-1,6-branch-beta-1,3-glucan derived from the skirting mushroom prepared in Preparation Example 3.

Formulation Example 1: Softener (Skin Lotion)

As shown in the following table, the softening lotion was prepared according to a conventional method.

ingredient Content (% by weight) Nano liposome 1.0 1,3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 2: Nutrients (Milk Lotion)

Nutrients were prepared according to a conventional method as shown in the table below.

ingredient Content (% by weight) Nano liposome 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocopheryl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Formulation Example 3: Nutrition Cream

Nutritious cream was prepared according to a conventional method as shown in the following table.

ingredient Content (% by weight) Nano liposome 10.0 Cetostearyl alcohol 2.0 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 Bietchiti 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.1 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 4: Massage Cream

Massage cream was prepared according to a conventional method as shown in the table below.

ingredient Content (% by weight) Nano liposome 1.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation Example 5: Pack

Packs were prepared according to conventional methods as shown in the table below.

ingredient Content (% by weight) Nano liposome 2.0 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 PEG-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

As described in detail above, the present invention provides a cosmetic composition for preventing skin aging containing nanoliposomes stabilized by inclusion of beta-1,6-branch-beta-1,3-glucan as an active ingredient. The cosmetic composition of the present invention can be usefully used to prevent skin aging by improving the skin permeation of the constant ingredient by stabilizing the beta-1,6-branch-beta-1,3-glucan derived from the skirt mushroom in the nanoliposomes have.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that the specific technology is merely a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (15)

Cosmetic composition for preventing skin aging containing nanoliposome stabilized by inclusion of beta-1,6-branched-beta-1,3-glucan (β-1,6-branched-β-1,3-glucan) as an active ingredient . The cosmetic composition for preventing skin aging according to claim 1, wherein the nanoliposome is contained in an amount of 0.1-20.0% by weight based on the total weight of the cosmetic composition. The cosmetic composition for preventing skin aging according to claim 1, wherein the beta-1,6-branch-beta-1,3-glucan is contained in an amount of 0.001-40.0 wt% based on the total weight of the nanoliposome. The cosmetic composition for preventing skin aging according to claim 1, wherein the beta-1,6-branch-beta-1,3-glucan is a component derived from Schizophyllum commune . The cosmetic composition according to claim 1, wherein the cosmetic composition (i) promotes skin penetration of beta-1,6-branch-beta-1,3-glucan derived from a skirt mushroom, (ii) increases skin moisturization, and (iii) enhances skin elasticity. (iv) increasing skin thickness, (v) improving skin wrinkles, (vi) reducing skin irritation, or (vii) recovering skin damage. The cosmetic composition for preventing skin aging according to claim 1, wherein the nanoliposome has an average particle diameter of 100.0-300.0 nm. The method of claim 1, wherein the nanoliposomes are prepared by a mixture comprising (i) a polyol, (ii) a surfactant, (iii) a phospholipid, (i) an oily component, (v) a fatty acid and (iii) water. Characterized in that the mixture does not contain a monohydric alcohol. 8. The method of claim 7, wherein the polyol is selected from the group consisting of propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isoprene glycol, pentylene glycol, erythritol, xyitol, sorbitol, and mixtures thereof. And, the content is a cosmetic composition for preventing skin aging, characterized in that 30.0-70.0% by weight relative to the total weight of nanoliposomes. 8. The surfactant according to claim 7, wherein the surfactant is (i) an anionic surfactant selected from the group consisting of alkylacylglutamate, alkyl phosphates, alkyl lactylates, dialkyl phosphates, alkyl lactates and trialkyl phosphates, (ii) alkoxy A nonionic surfactant selected from the group consisting of a rated alkyl ether, an alkoxylated alkyl ester, an alkyl polyglycoside, a polyglyceryl ester and a sugar ester or (iii) a mixture of the anionic surfactant and the nonionic surfactant , The content is 0.3-5.0% by weight relative to the total weight of the liposomes, the anti-aging cosmetic composition. The method of claim 7, wherein the phospholipid is lecithin, the content of the cosmetic composition for preventing skin aging, characterized in that 2.0 to 8.0% by weight relative to the total weight of the nanoliposomes. According to claim 7, wherein the oily component is selected from the group consisting of hydrocarbon-based oils, ester-based oils, silicone oils, animal and vegetable oils and mixtures thereof, the content is 3.0-20.0% by weight relative to the total weight of nanoliposomes Cosmetic composition for preventing skin aging, characterized in that. 8. The cosmetic composition for preventing skin aging according to claim 7, wherein the fatty acid is a saturated or unsaturated fatty acid of a C _12-22 alkyl chain, and its content is 0.1-1.0 wt% based on the total weight of the nanoliposomes. The method of claim 7, wherein the water is deionized distilled water, the content of the anti-aging cosmetic composition, characterized in that 3.0 to 20.0% by weight relative to the total weight of the nanoliposomes. The cosmetic composition for preventing skin aging according to claim 7, wherein the nanoliposomes are prepared by applying the mixture to a high pressure homogenizer. The group of claim 1 wherein the cosmetic composition comprises a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing oil, a powder foundation, an emulsion foundation, a wax foundation and a spray Cosmetic composition for preventing skin aging, characterized in that it has a formulation selected from.