KR101580107B1

KR101580107B1 - Cosmetic composition containing swallow's nest extract fermented by mushroom mycelium

Info

Publication number: KR101580107B1
Application number: KR1020150081279A
Authority: KR
Inventors: 김윤정; 최성규; 김다애; 이대우; 김동욱
Original assignee: (주)더마랩
Priority date: 2015-06-09
Filing date: 2015-06-09
Publication date: 2015-12-28

Abstract

The present invention relates to a cosmetic composition containing a fermented extract of a swallow′s nest with mushroom mycelia as an active ingredient and, more specifically, to a cosmetic composition with excellent skin wrinkle amelioration, skin moisturization, inflammation relief, skin cell proliferation, and skin irritation relief effects, by containing an extract as the active ingredient, wherein the extract is produced by culturing mushroom mycelia, and then further culturing the swallow′s nest on the mushroom mycelia thereafter.

Description

The present invention relates to a cosmetic composition containing swallow's nest extract fermented by mushroom mycelium,

The present invention relates to a cosmetic composition comprising as an active ingredient an extract of a fermented bird's nest of mushroom mycelium. More specifically, the present invention relates to a cosmetic composition having an excellent skin-improving activity by preparing an extract after culturing a bird's nest on a mushroom mycelium, To a manufacturing technique.

The bird's nest extract is one of the newest ingredients of cosmetics that are attracting attention and is a raw material having anti-aging effect such as skin elasticity and moisturizing. The bird's nest was used for skin beauty in China and the poppy was used for skin beauty. Its effect is known and its history is long enough to be used as a dish for longevity in Chinese imperial court. For this reason, the bird's nest extract has been used in cosmetics and food fields and is expected to be used for a long time in the future.

The mushroom mycelium contains nutrients to the fruiting body and has strong active ingredients as it keeps the energy to grow fruiting bodies. Therefore, it contains many active ingredients and it is effective. Therefore, many products using mushroom mycelium have been developed.

Korean Patent Laid-Open No. 10-2010-0088880 entitled " composition for external application for skin containing fermented herbal extracts "includes " Gakyung, licorice, Extracting an extract from a crude drug mixture consisting of peppermint, herbicide and celadon; Mixing the extract with a pre-culture solution of mycelia of leaf mushroom (KCTC 10337BP) or mycelia of leaf mushroom (KCTC 10337BP), and then culturing it; (KCTC 10337BP) mycelia from the present culture medium after the present cultivation, as an active ingredient.

While studying various natural products having skin improving activity, the inventors of the present invention have studied the activity of producing a bird's nest extract using mushroom mycelium. The produced mushroom mycelium fermented bird's nest extract showed excellent skin improving effect, And thus the present invention has been completed.

It is an object of the present invention to provide a cosmetic composition containing as an active ingredient an extract of a fermented larva of mushroom mycelium.

It is another object of the present invention to provide a method for preparing an extract of a fermented nectar from a mushroom mycelium excellent in skin wrinkle, skin moisturizing, inflammation relief and skin cell proliferation.

According to an aspect of the present invention, there is provided a method for producing a mushroom mycelium, which comprises culturing a mushroom mycelium selected from the group consisting of a mushroom, a skirt mushroom, a mushroom, a shiitake mushroom, a red mushroom, a ginseng mushroom and a caterpillar fungus; Culturing the mushroom mycelium by cultivating the nursery; And a step of recovering and then collecting the nursery covered with the mushroom mycelium, and a cosmetic composition containing the extract of the fermentation nasturtium of the mushroom mycelium produced. Preferably, the mushroom mycelium is a mycelia of Ganoderma lucidum, Pleurotus ostreatus or Rhodiola mushroom, and more preferably a mycelium of Rhizoma mushroom.

The mushroom mycelium fermentation nematode extract is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition. Wherein the extract is at least one selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diethyl ether It is an extract by solvent. The cosmetic composition exhibits an effect of improving skin wrinkles, skin moisturizing, improving skin irritation, proliferating skin cells, and alleviating skin irritation.

According to another aspect of the present invention, there is provided a mushroom mycelium selected from the group consisting of (A) mushroom, skirt mushroom, oyster mushroom, shiitake mushroom, Followed by culturing at a temperature of 25 to 35 DEG C, preferably 27 to 30 DEG C for 5 to 10 days to produce mushroom mycelium; (B) cultivating the nursery on a mushroom mycelium at a temperature of 27 to 30 DEG C for 3 to 5 days; (C) recovering the nursery covered with mushroom mycelium; And (D) extracting the recovered bird's nest. In the step (B), it is preferable to use a bird's nest with water in the bird's nest for 1-3 hours.

Preferably, the extraction in step (D) is performed by mixing the recovered nascent mushroom-covered bird's nest with a mixture solvent of 5 to 10 times by weight of 1,3-butylene glycol and purified water in a ratio of 3: 7 (w / w) And it is immersed for 3-5 days and extracted from the extractor with cooling condenser by boiling at 100 ~ 120 ° C for 8 ~ 10 hours, followed by filtration.

The extract of the mushroom mycelium fermentation birdhouse according to the present invention is excellent in skin wrinkle, skin moisturizing, inflammation relief, skin cell proliferation and skin irritation mitigating effect, and the cosmetic containing the extract as an active ingredient is useful as a cosmetic for skin improvement .

Hereinafter, the present invention will be described in more detail.

TECHNICAL FIELD The present invention relates to a technique for preparing a cosmetic composition containing an extract of fermented larvae of mushroom mycelium as an active ingredient.

Mushrooms belong to the family Mycobacterium, a kind of higher fungi, and their biological location belongs to fungi. The mushroom is germinated by spores, forming mycelium (Mycelium), growing as a fruit body (mushroom), and fruiting bodies grow again spore formation process. The seeds of the mushroom are in the form of spores in the freshly wrinkled flesh of the fruiting body. When the spores start to germinate in the medium, the seeds of the fungus are extracted and the nutrients of the surrounding medium are absorbed and proliferated continuously for about 3 to 4 months. do. Mycelium and fruiting bodies have significant differences in function, structure and composition.

The mushroom mycelium contains nutrients to the fruiting body and has strong active ingredients as it keeps the energy to grow fruiting bodies. Therefore, it contains many active ingredients and it is effective. Mycelium contains about 4 times more nutrients than fruiting bodies. It is rich in protein, amino acids, vitamins, minerals and enzymes. Mycelium has excellent absorption and propagation ability and therefore has much more energy than mushroom edible parts. . Therefore, the core of the energy of the mushroom can be seen in the mycelium.

The nest of birds was considered to be rare from ancient times as a nest that was formed by hardening the saliva of a swallow, a kind of swallow. In addition, the bird's nose has a lubrication effect that adds radiance to the skin and has been consumed for skin beauty and moisturizing. The bird's nest was the royal dish of Vietnam, which was brought to China and received a great deal of help from the kings of the Chinese dynasties. The bird's nest is the main ingredient of seaweed and fish fin, and its ingredient is known as a ginseng protein, which is known as a recipe for ginseng protein, and " It was excelled in the effect of recreation, and the Chinese Emperor used it as skin beauty to combine sea birds and pearl powder to maintain its beauty and youth.

The mushroom mycelium and the nest of the mushroom mycelium fermented nematode extract were prepared and evaluated for its activity. As a result, the mushroom mycelium exhibited excellent skin moisturizing and skin-improving activity, and the mushroom mycelium was cultured to produce a bird's nest extract , Skin wrinkle improvement, skin irritation mitigation, skin cell proliferation and skin irritation mitigation activity were found to be particularly excellent.

According to one embodiment of the present invention, a mushroom mycelium fermented nematode extract having excellent skin improving activity activity is prepared by the following method.

First, the mushroom mycelium is cultured in a Potato Dextrose Agar medium at 25 to 35 ° C., preferably 27 to 30 ° C. for 5 to 10 days. A bird's nest is placed on the obtained mushroom mycelium. In this case, if the fermentation is sufficiently performed, the amount of the bird's nest provided for the culture is not limited. However, according to one embodiment of the present invention, a 1 x 1 cm bird's nest is placed on a Potato Dextrose Agar medium prepared in a Petri dish of 90 x 15 mm size I put ~ 10 pieces.

It is preferable that the bird's nest used is clean water for 1 to 3 hours so as to have moisture. The nursery, which is placed on the mycelium of mushrooms, is fermented at a temperature of 27 ~ 30 ℃ for 3 ~ 5 days.

After the cultivation, the nursery covered with mushroom mycelium is recovered and extracted with a solvent of 5-10 times weight. At least one solvent selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diethyl ether is used According to one preferred embodiment of the present invention, 3-butylene glycol and purified water are extracted with a solvent mixed at a ratio of 3: 7 (w / w) to prepare a fermented nematode extract of mushroom mycelium.

At this time, the mushroom mycelium is selected from mushroom mycelia selected from the group consisting of mushroom, skirt mushroom, oyster mushroom, shiitake mushroom, red mushroom, gingko mushroom and caterpillar fungus. Preferably, the mushroom mycelium, oyster mushroom, , And more preferably, a skins mushroom mycelium is used to produce a bird's nest extract.

The present invention relates to a method of fermenting a fermented product by putting a nest on a specific mushroom mycelium and culturing it, instead of fermenting the fermented product by first preparing the herbal medicine extract to be fermented, putting the herbal medicine extract in the liquid medium and inoculating the mushroom mycelium Use features.

The thus obtained mushroom mycelial fermentation nevus extract showed excellent effects of increasing the expression of wrinkle-related factors (Test Example 1), increasing the expression of moisturizing factors (Test Example 2), suppressing the expression of inflammatory mediators (Test Example 3) (Test Example 4) and skin irritation alleviation effect (Test Example 5).

The mushroom mycelium fermentation nematode extract as an active ingredient is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition.

The cosmetic composition may be prepared in any conventional formulation, and examples thereof include lotion, cream, essence, cleansing foam, cleansing water, pack, body lotion, body oil, body gel, shampoo, rinse, hair conditioner, Hair gel, foundation, lipstick, mascara, make-up base and the like.

[Example]

Hereinafter, the present invention will be described in detail by way of the following examples and test examples, but the present invention is not limited to these examples.

Production Examples 1 to 3: Production of mycelia of mushroom

First, Ganoderma lucidum, Pleurotus ostreatus and Chamaecyparis obtusa were used as a strain and subcultured on a plate medium of Potato Dextrose Agar (PDA, DIFCO, USA). Mushroom mycelia were cultured for 10 days at 30 ℃ in PDA medium.

Production Examples 4 to 6: Production of a mycelium fermentation bird of mushroom

The cultivated Ganoderma mushroom, Pleurotus ostreatus, and Mycelium of skimmed mushrooms were used to prepare a bird's nest extract. The bird's nest was kept in water for 3 hours in clean water and placed on the mycelia of Ganoderma lucidum, Oyster mushroom and Rhizoma mushroom, and then cultured at 27 ° C for 10 days. After the cultivation, the nursery was covered with mycelium of Ganoderma lucidum, Pleurotus ostreatus, and Rhizoma mushroom, and each 1 g of mycelial fermentation vivarium was prepared.

Examples 1 to 3: Preparation of an extract of fermented bird of mushroom mycelium

1 g of each of the collected fermented vaginal yeast mushroom, Pleurotus ostreatus and Rhizoma mushroom collected in Production Examples 4 to 6 was mixed with 10 g of a mixed solvent of 1,3-butylene glycol and purified water at a ratio of 3: 7 (w / w). The mixture was immersed for 3 days and boiled for 8 hours at 100 ° C in an extractor equipped with a cooling condenser. The mixture was filtered using a filter paper (Qualitative Filter Papers No. 2), and then filtrated.

Comparative Example 1: Production of bird's nest extract

1 g of the bird's nest was mixed with 10 g of a mixed solvent of 1,3-butylene glycol and purified water in a ratio of 3: 7 (w / w). The mixture was immersed for 3 days and boiled for 8 hours at 100 ° C in an extractor equipped with a cooling condenser. The mixture was filtered using a filter paper (Qualitative Filter Papers No. 2), and then filtrated.

Test Example 1: Confirmation of Increasing Expression of Wrinkle-Related Factor

Fibroblast (CCD-986Sk) was adjusted to 5 × 10 ⁵ cells / well using IMDM medium supplemented with 10% FBS, and then inoculated on a 12-well plate and cultured for 18 hours. After culturing, the samples of the above Examples and Comparative Examples were treated and cultured for 24 hours. RNA was extracted from the cultured cells and the increase rate of collagen expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The increase rate of collagen expression was calculated by the following equation, and the results are shown in Table 1 below. As a control group, adenosine was used.

Collagen expression rate (%) = (sample collagen expression level / control collagen expression level) × 100

Name of sample Treatment concentration (w / v%) Collagen expression rate (%) Example 1 0.5 82.0 Example 2 0.5 84.3 Example 3 0.5 92.5 Comparative Example 1 0.5 80.3 Adenosine 0.05 83.8

As shown in the above Table 1, the mushroom mycelial fermentation bird's nest extracts of Examples 1 to 3 exhibited an excellent collagen expression effect than the bird's nest extract of Comparative Example 1 at the same concentration treatment, and exhibited equivalent or superior effects compared with adenosine .

It was also confirmed that the extract of the fermented bird house fermented mushroom mycelium of Example 3 exhibited a better collagen expression enhancement effect than that of the fermented nectar of fermented mushroom and oyster mushroom.

Test Example 2: Confirmation of increasing effect of moisturizing factor

Fibroblast (CCD-986Sk) was adjusted to 5 × 10 ⁵ cells / well using IMDM medium supplemented with 10% FBS, and then inoculated on a 12-well plate and cultured for 18 hours. After culturing, the samples were treated and incubated for 24 hours. RNA was extracted from the cultured cells and the expression of Hyaluronan Synthase 3 (HAS-3) was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The HAS-3 expression increase rate was calculated by the following equation, and the results are shown in Table 2 below. As a control group, hyaluronic acid was used.

HAS-3 expression ratio (%) = (expression amount of sample HAS-3 / expression amount of control HAS-3) × 100

Name of sample Treatment concentration (w / v%) Expression rate of HAS-3 (%) Example 1 0.5 85.1 Example 2 0.5 87.4 Example 3 0.5 93.5 Comparative Example 1 0.5 83.1 Hyaluronic acid
(Hyaluronic Acid) 0.05 84.0

As shown in the above Table 2, the mash mycelium fermentation larvae extracts of Examples 1 to 3 exhibited superior HAS-3 expression compared to the nasturtium extract of Comparative Example 1 at the same concentration treatment, and were comparable or superior to hyaluronic acid Effect.

In addition, it was confirmed that the extract of the fermented bird house fermented mushroom mycelia of Example 3 exhibited a better HAS-3 expression increase effect than the fermented bird house fermented extract of Ganoderma mantis and Oyster mushroom.

Test Example 3: Effect of reducing inflammatory mediator expression

RAW 264.7 cells were adjusted to 5 × 10 ⁵ cells / well using DMEM medium supplemented with 10% FBS, and then inoculated on a 12-well plate and cultured for 18 hours. After incubation, starvation was maintained for 12 hours, LPS 10 ng / mL and samples were treated and incubated for 24 hours. RNA was extracted from the cultured cells and the inhibition rate of TNF-α expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The inhibition rate of TNF-a expression was calculated by the following formula, and the results are shown in Table 3 below. Dexamethasone was used as a control.

Inhibition rate (%) of TNF-α expression = 100 - [(expression amount of sample TNF-α / expression amount of control group TNF-α) × 100]

Name of sample Treatment concentration (w / v%) TNF-α expression inhibition rate (%) Example 1 0.5 63.2 Example 2 0.5 69.5 Example 3 0.5 79.7 Comparative Example 1 0.5 65.8 Dexamethasone
(Dexamethason) 0.05 66.5

As shown in the above Table 3, the mushroom mycelial fermentation larvae extracts of Examples 1 to 3 exhibited a TNF-α inhibitory effect superior to that of Comparative Example 1 at the same concentration, and were equivalent or superior to dexamethasone Effect.

Test Example 4: Confirmation of skin cell proliferation effect

A wound healing assay was performed to confirm the skin cell proliferation effect of the mushroom mycelium fermentation ranunculus extract. The keratinocyte (HACAT) was adjusted to 5 × 10 ⁵ cells / well using DMEM supplemented with 10% FBS, and then inoculated on a 6-well plate and cultured for 18 hours. The cells grown on the 6 well plate were scratched to remove a part of the cells from the bottom of the 6-well plate, and the samples of the above-mentioned Examples and Comparative Examples were treated and cultured for 18 hours. The degree of cell proliferation was confirmed, and the results are shown in Table 4 below.

The cell proliferation rate was calculated by the following equation, and dexamethasone was used as a control group.

Cell proliferation rate (%) = (sample cell proliferation rate / control cell proliferation rate) x 100

Name of sample Treatment concentration (w / v%) Cell proliferation rate (%) Example 1 0.5 83.7 Example 2 0.5 83.4 Example 3 0.5 93.2 Comparative Example 1 0.5 85.1 Dexamethasone
(Dexamethason) 0.5 85.0

As can be seen in Table 4 above, the mushroom mycelial fermentation bird house extracts of Examples 1 to 3 exhibited superior skin cell proliferation effect than the bird house extract of Comparative Example 1 at the same concentration treatment, and showed equivalent or superior effects compared with dexamethasone Respectively. The fermented avian extract from Rhizoma mushroom mycelium of Example 3 showed particularly excellent skin cell proliferation effect.

Example 4: Production of lotion containing fermented bird's nest extract of mushroom mycelium

The lotion containing the mushroom mycelium fermented nevus extract prepared in the above Example was prepared according to a conventional method with the composition and the content shown in Table 5 below.

(weight%) Control Example Comparative Example 2 Example 4 Comparative Example 1 - 0.5 - Example 3 - - 0.5 Wax 1.0 1.0 1.0 Polysorbate 60 1.5 1.5 1.5 Sorbitan sesquioleate 0.5 0.5 0.5 Liquid paraffin 10.0 10.0 10.0 Sorbitan stearate 0.5 0.5 0.5 Pro-type glycerin monostearate 0.5 0.5 0.5 Stearic acid 1.5 1.5 1.5 Glyceryl stearate /
FAGE-400 stearate 1.0 1.0 1.0 Propylene glycol 3.0 3.0 3.0 Carboxy polymer 0.1 0.1 0.1 Triethanolamine 0.2 0.2 0.2 antiseptic a very small amount a very small amount a very small amount Purified water To 100 To 100 To 100

Test Example 5: Confirmation of skin irritation mitigation effect

The present invention relates to a method for producing a mushroom mycelium-containing fermented nevus extract, comprising the steps of: (a) providing a skin stimulation through a tape stripping method on the inferior part of 10 persons, The degree of stimulation induced by applying a cosmetic composition and a cosmetic composition not containing the cosmetic was relatively evaluated. First, taping was repeated 30 times with a scotch tape on both sides of the underbody to cause skin erythema. After erythema induction, the cosmetic composition containing the mushroom mycelium fermentation bird house extract according to the present invention was applied and the cosmetic composition containing no extract according to the present invention was applied. The degree of skin irritation was evaluated 3 hours after application.

&Lt; Evaluation of degree of skin irritation (erythema) >

0 (no response) 1 (very mild erythema) 2 (faint or mild erythema)

3 (clear erythema but weak erythema) 4 (pronounced erythema) 5 (severe erythema)

Name of sample Degree of skin irritation Left Right Control Example One 2 Comparative Example 2 One 0 Example 4 0 0

As shown in Table 6, the lotion composition containing the extract of the fermented bird of the mushroom mycelium of the present invention as an effective ingredient was superior to the control and the Comparative Example 2 which did not contain the fermentation nematode aspirate.

Claims

Culturing the mycelia of mycelium of mushrooms subculture on a PDA (Potato Dextrose Agar) plate culture medium and culturing the mushroom mycelium at a temperature of 25 to 35 DEG C for 5 to 10 days; Culturing the cultured mushroom mycelia in water at a temperature of 27 to 30 ° C for 3 to 5 days; And the mushroom mycelium-covered vesicles were collected and then immersed in a mixed solvent of 1,3-butylene glycol and purified water having a weight of 5 to 10 times for 3 to 5 days and boiled at 100 to 120 ° C for 8 to 10 hours in an extractor equipped with a cooling condenser And extracting and filtering the mushroom mycelium fermented nematode extract.

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The cosmetic composition for skin moisturizing according to claim 1, wherein the mushroom mycelium fermentation nematode extract is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition.

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(A) culturing mycelia of skimmed mushrooms subcultured on a PDA (Potato Dextrose Agar) plate culture medium and culturing at a temperature of 25 to 35 DEG C for 5 to 10 days to prepare mushroom mycelium;
(B) cultivating the nursery on a mushroom mycelium at a temperature of 27 to 30 DEG C for 3 to 5 days;
(C) recovering the nursery covered with mushroom mycelium; And
(D) The collected novile mushroom-covered bird's nest was mixed with a mixed solvent of 5 to 10 times by weight of 1,3-butylene glycol and purified water in a ratio of 3: 7 (w / w), and the mixture was immersed for 3 to 5 days, And extracting the beans by boiling at 100 to 120 ° C for 8 to 10 hours, followed by filtration, thereby producing a fermented nematode extract of mushroom mycelium.

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