KR101336804B1 - Fermented ear shell using mushroom, manufacturing method thereof and cosmetic composition comprising the same - Google Patents

Abstract

The present invention relates to a fermented ear shell using mushrooms which is obtained by fermenting the ear shell using a mushroom mycelium and a cosmetic composition including the same for moisturizing, firming, whitening, and improving wrinkles. The fermented ear shell is obtained by fermenting the ear shell using a mushroom mycelium. The mushroom mycelium is selected from a group including Ganoderma lucidum mycelium, Phellinus linteus mycelium, Hericium erinaceum mycelium, Pleurotus cystidiosus mycelium, and a mixture of two or more of the above mycelia.

Description

Fermented mushrooms abalone mushroom using mushrooms, a method for manufacturing the same and a cosmetic composition comprising the same {Fermented ear shell using mushroom, manufacturing method

The present invention relates to an abalone mushroom fermented extract, a preparation method thereof, and a cosmetic composition comprising the same, in particular, an abalone mushroom fermented product obtained by fermenting abalone with mushroom mycelium and moisturizing, elasticity, whitening, and skin wrinkles comprising the same as an active ingredient. It relates to a cosmetic composition for improvement.

Fermentation refers to a process in which microorganisms decompose or synthesize organic matter using their own enzymes. Representative fermentation microorganisms include Bacillus subtilis, lactic acid bacteria, yeast bacteria, yeast, mold, acetic acid bacteria, and mushroom mycelium.

Recently, as the interest in fermented foods and cosmetics using natural materials has increased in the cosmetics field, researches on whitening, skin wrinkle improvement, antioxidant, moisture, elasticity, etc. are active.

The fermented product or fermented product extract does not use any other solvents, and after fermentation and ripening, the sugar component contained in the natural material is converted into alcohol, and flavonoids, vitamin C, vitamin P, amino acids, Extraction efficiency for catechins and other active ingredients can be improved. In addition, sometimes irritating or toxic natural materials can be used as a method of stabilizing or reducing the natural materials through the fermentation method or converting them into stable derivatives, it is possible to obtain a much better cosmetic effect. In addition, by the fermentation process such as lactic acid bacteria and yeast, it is possible to maximize the efficacy of natural materials in the process of conversion to low molecular weight materials that are easily absorbed, from the unstable or inactive form to the active form, to enhance moisturizing and elasticity, whitening, skin Wrinkle improvement effect can be maximized.

The abalone (ear shell ( Haliotis discus hannai )) is a general term for mollusks belonging to the abalone family of primitive abalone, and it is aquatic organisms that live near the clean reefs and feed algae. It is rich in vitamins B1, B2 and protein, and it is effective in skin care, nourishing tonic and postpartum cooking. In particular, it is rich in taurine, which is effective in protecting liver, recovering from fatigue, myocardial infarction, etc., protecting liver and kidney, clearing eyes, opening stomach, controlling sea water, nourishing tonic, headache, dizziness, etc. There is a high content of collagen, thereby rejuvenating the cells and activating the skin metabolism to help skin beauty. As a high-quality seafood, it has been characterized as incomparable to other seafoods in terms of nutrition and taste since ancient times, and has been used as a healthy food in Korea, but it is not popularized because of its high price.

Most abalones on the market are 1 to 4 years old, and more than 3 years old are used for food such as abalone porridge. However, according to a comparative study of physicochemical characteristics of meat and intestines according to the age of abalone, the contents of nutrients and active ingredients such as taurine, fatty acids and chondroitin sulfate are different according to the age of abalone. The age of is considered to be considered when selecting abalone as a raw material.

Studies on abalone include comparison of physicochemical properties of meat and intestines according to the age of abalone, immune enhancing composition containing abalone viscera extract as an active ingredient, cosmetic composition for improving skin wrinkles containing abalone extract as an active ingredient, abalone extract Cosmetic composition and its preparation method, immune enhancing composition comprising the abalone viscera extract, food composition for improving and preventing liver disease containing shellfish extract, abalone digestion hydrolyzate having active oxygen species scavenging activity, abalone extract Beverage composition and its preparation method, hangover quenching beverage containing abalone lyophilized meat and its manufacturing method, functional cosmetic composition using natural ingredients, method of manufacturing steamed dried abalone and steamed dried abalone produced by it, lactic acid and grapefruit seed extract Antimicrobial composition and food processing method of abalone using same And cosmetics as well as expanding the field of cosmetics, but is still in the introduction phase, there is no research on the fermented extract of abalone that can maximize the active ingredient through the fermentation process.

Abalone Pleurotus cystidiosus belongs to the Plerotaceae family and is named because it resembles abalone, and is called by O. Miller (named Pleurotus cystidiosus by OK Miller), maple beetle , and Taiwan beetle . Also do. While the common lodge has a medium and low temperature of cultivation temperature of 25 ° C. or lower, abalone is also generated at a high temperature of 25 to 30 ° C. The optimal cultivation time in Korea is when the average temperature is 20 to 28 ℃, that is from mid-June to early September. The morphological features of abalone elk are flat in shape, 6.6 to 9.8 cm in size, and 1.6 to 3.8 cm in length. . It is not formed in the form of a bundle like a circular blade, but is generated individually, and the average is about 12.8g. The shade is dark brown, and the off-white color is dark, but the color is dark and light due to temperature and light.

In this regard, a composition for anti-aging and skin wrinkle improvement (patent application No. 2010-0008638) containing an extract of Abalone mushroom mycelium or fruiting body as an active ingredient is disclosed.

In addition, recently, as a substrate, a cosmetic raw material for skin whitening of silkworm Cordyceps sinensis mycelium culture medium using the mulberry leaf extract of Mulberry leaf extract and silkworm Cordyceps sinensis mycelium, and a fresh herb consisting of fresh vinegar, eochocho and triticale Development of various cosmetic raw materials using cultures obtained by culturing mushroom mycelium on natural medium such as whitening cosmetics containing mushroom mycelium culture of Korean Patent Publication No. 10-0848515 to inoculate and incubate mushroom mycelium on It is becoming.

Accordingly, the present invention relates to a cosmetic composition for exploring the biologically active material of the abalone extract, and to enhance the content and functionality of the active ingredient through the mushroom mycelium fermentation process, moisturizing, elasticity, A cosmetic composition for whitening and improving skin wrinkles has been developed.

An object of the present invention is to provide a cosmetic composition for moisturizing, elasticity, whitening, skin wrinkle improvement comprising the abalone mushroom fermentation obtained by fermenting abalone with mushroom mycelium as an active ingredient.

In order to achieve the above object, the present invention makes mushroom abalone mushroom fermented product through the fermentation process using mushroom mycelium, moisturizing, elasticity containing the abalone mushroom fermented product using this abalone mushroom fermented product in the manufacture of cosmetics Provides a cosmetic having whitening, skin wrinkle improvement activity.

The abalone mushroom fermentation product which concerns on this invention is obtained by fermenting abalone with mushroom mycelium.

The abalone may include both abalone muscle and abalone viscera.

The mushroom mycelia are mycelium of Ganoderma lucidum , mycelium of Phellinus linteus , mycelium of Hericium erinaceum , mycelium of abalone mushroom ( Pleurotus cystidiosus ) and a mixture of two or more of them May be selected from.

Method for producing an abalone mushroom fermented product according to the invention, (1) inoculation step of inoculating abalone with mushroom mycelium; And (2) a fermentation step of fermenting the abalone inoculated with mushroom mycelium.

The abalone in the inoculation step may include both abalone meat and abalone internal organs.

The mushroom mycelium at the inoculation step is mycelium of Ganoderma lucidum , mycelium of Phellinus linteus , mycelium of Hericium erinaceum , mycelium of Pleurotus cystidiosus and two of them It may be selected from the group consisting of the above mixture.

A pretreatment step of quenching and lyophilizing the abalone may be further performed before the inoculation step.

Between the pretreatment step and the inoculation step, the dried abalone obtained in the pretreatment step may be further mixed and mixed with water to form a culture solution to form a culture solution for culturing mycelium.

The fermentation step may be followed by an aging step of aging the fermentation broth obtained in the fermentation step.

The aging step may be performed by storing the fermentation broth at 3 to 10 ° C. for 1 to 3 days.

A post-treatment step may be further performed in which the fermentation liquid obtained after the fermentation step or the aging step is concentrated under reduced pressure and lyophilized to a water content of 10% to obtain a lyophilized fermentation product.

Cosmetic composition comprising the abalone mushroom fermented product using the mushroom according to the present invention in an amount within the range of 0.001 to 80% by weight of the lyophilized fermented product obtained by lyophilization as an active ingredient based on the total weight of the cosmetic composition. Include.

According to the present invention, there is provided an abalone mushroom fermented product, a preparation method thereof, and a cosmetic composition comprising the same as an active ingredient, wherein the abalone mushroom fermented product includes an active ingredient including controchin sulfate, and a fermentation process using mushroom mycelium. The fermentation broth produced through the fermentation process is converted into a low molecular weight compound by the enzyme produced during the fermentation process, which increases the useful substances for the skin, and the active ingredients of the mycelium are combined to bring about moisturizing, elasticity, and It provides an effect that is useful in manufacturing and the like.

In addition, the cosmetic composition containing the abalone mushroom fermentation is excellent in antioxidant activity, the production of collagen, the inhibitory effect of MMP-1 (Matrix metalloproteinase; enzymes that promote the breakdown of collagen) to exert the effect of improving skin wrinkles to provide.

Hereinafter, the present invention will be described in detail with reference to specific examples.

The present invention uses the mushroom mycelium to make the abalone mushroom fermentation through the fermentation process, and using this abalone mushroom fermentation in the manufacture of cosmetics moisturizing, elasticity, whitening, skin wrinkle improvement activity containing such abalone mushroom fermentation To provide cosmetics.

The abalone mushroom fermented product according to the present invention is characterized by being obtained by fermenting abalone with mushroom mycelium.

The abalone may include both abalone muscle and abalone viscera.

The mushroom mycelia are mycelium of Ganoderma lucidum , mycelium of Phellinus linteus , mycelium of Hericium erinaceum , mycelium of abalone mushroom ( Pleurotus cystidiosus ) and a mixture of two or more of them May be selected from.

Method for producing an abalone mushroom fermented product according to the invention, (1) inoculation step of inoculating abalone with mushroom mycelium; And (2) a fermentation step of fermenting the abalone inoculated with mushroom mycelium.

The abalone in the inoculation step may include both abalone meat and abalone internal organs.

The mushroom mycelium at the inoculation step is mycelium of Ganoderma lucidum , mycelium of Phellinus linteus , mycelium of Hericium erinaceum , mycelium of Pleurotus cystidiosus and two of them It may be selected from the group consisting of the above mixture.

A pretreatment step of quenching and lyophilizing the abalone may be further performed before the inoculation step.

Between the pretreatment step and the inoculation step, the dried abalone obtained in the pretreatment step may be further mixed and mixed with water to form a culture solution to form a culture solution for culturing mycelium.

The fermentation step may be followed by an aging step of aging the fermentation broth obtained in the fermentation step.

The aging step may be performed by storing the fermentation broth at 3 to 10 ° C. for 1 to 3 days.

A post-treatment step may be further performed in which the fermentation liquid obtained after the fermentation step or the aging step is concentrated under reduced pressure and lyophilized to a water content of 10% to obtain a lyophilized fermentation product.

Cosmetic composition comprising the abalone mushroom fermented product using the mushroom according to the present invention in an amount within the range of 0.001 to 80% by weight of the lyophilized fermented product obtained by lyophilization as an active ingredient based on the total weight of the cosmetic composition. Include.

Method for producing the abalone mushroom fermentation product according to the invention preferably comprises the steps of (1) quenching and freeze-drying of abalone meat and intestines; (2) a culture solution composition step of culturing the mycelium by mixing the dried abalone meat and viscera pulverized with water; (3) fermenting by inoculating the mushroom mycelium alone; And (4) fermentation broth obtained from the step of aging the fermentation broth, or concentrates obtained by partially or totally depressurizing and lyophilizing the fermentation broth from the fermentation broth, or chemical substances exerting the main effect contained in the fermentation broth and the concentrate. It includes itself.

The abalone was washed with water after separating the meat and the intestines, and stored in a rapid freeze at -80 ℃ lyophilized and then pulverized to prepare a fine powder form. By lyophilizing and drying the abalone powder, the abalone can be stored for a long time while maintaining the nutritional and functional ingredients of the abalone, and it is easy to handle during storage. It is possible to supply the raw materials of aquaculture three to four year old abalone.

The abalone mushroom fermented product was lyophilized and mixed with a powder sample crushed in water at a stable temperature, inoculated with mushroom mycelium alone to obtain a fermentation broth through a fermentation process. Alternatively, the fermentation process may include a low temperature aging or a high temperature aging process.

The mushroom mycelium used for fermentation of abalone mushrooms is mycelium of Ganoderma lucidum , mycelium of Phellinus linteus , mycelium of Hericium erinaceum , mycelium of Pleurotus cystidiosus It may be selected from the group consisting of two or more of the mixture. The mushroom mycelium is available for sale on the website of the mushroom strain and DNA bank (CCDBM) (http://www.wildmush.or.kr/sub/catalog.php?CatNo=23&Sort=).

The inoculation and fermentation step is to inoculate the mushroom mycelium (preculture) cultured in the fermenter containing the culture solution, inoculate the mushroom mycelium to 5 to 15% by weight. In the fermentation step, the culture solution inoculated with the preculture is fermented for 3 to 10 days under optimum conditions (temperature 24 to 28 ° C). Mushroom mycelium cultured in the culture tank containing the culture solution may have a slight difference in the temperature and duration of the culture and fermentation depending on the type of the seed, but for those skilled in the art to select the appropriate fermentation conditions It can be easily understood.

The aging step is a step of fermenting the abalone extract inoculated mushroom mycelium, and the step of adding the product according to the commercialization method using the fermentation product (foot enzyme material) obtained by the step of obtaining a fermentation material, the fermentation material 3 to Aged at low temperature of 10 ℃ 1-3 days.

The cosmetic composition according to the present invention may contain, in addition to the above-mentioned extracts, other components that can give a synergistic effect to the main effect without impairing the main effect of the present invention. The abalone fermentation extract may be present in the composition in an amount of 0.001 to 80% by weight based on the total weight of the composition.

The abalone mushroom fermentation product according to the present invention can be processed into a solution or powder form in the form of nanocapsules, microcapsules, nanosomes, etc. using techniques known in the art, and those skilled in the art It will be possible to control the amount of this solution or powder in the composition according to the invention. As described above, the present invention is provided to cosmetics, foods, medicines, and the like processed into a composition having a whitening and skin wrinkle improving activity having a moisturizing and elasticity improving effect.

Abalone fermented extract of the present invention is not particularly limited in its formulation as an external preparation for skin, basic cosmetics and foundations such as softener, milk lotion, nutrition cream, massage cream, essence, cleansing foam, cleansing water, pack or body oil, It may be prepared in the form of a color cosmetic such as lipstick, mascara or makeup base. When the cosmetic of the present invention is used as a detergent, a cleanser and a bath agent can be produced.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the present invention is not limited thereto.

Example 1

Abalone purchased live abalone to remove foreign matters attached to the shell and then washed whole, separated meat and viscera to prepare abalone meat and abalone gut, and then washed once more. Rapid freeze storage at -80 ° C lyophilized and then ground to prepare a powder form.

After mixing 10 times the distilled water of the abalone meat and abalone viscera powder, mix well, and prepare the mycelium optimized for cultivation to inoculate the abalone extract with the precultured mushroom mycelium (Pear culture liquid; abalone mushroom mycelium). , The abalone mushroom mycelia were incubated at a temperature of 25 ℃, shaking rate (ShakingRate) 120 RPM for 3 days) to 10% by weight. Thereafter, the fermentation was carried out for 5 days under optimum conditions (temperature 26 ° C). In order to terminate the fermentation, heat was applied at 80 ° C. for 10 minutes to induce a malfunction of the mushroom mycelium. The fermented material was aged at a low temperature of 4 캜 for 2 days for aging. The precipitate was removed using a centrifuge, the supernatant was collected, or the filtered supernatant was concentrated under reduced pressure to a water content of 10% or less at 60 ° C or lower and then freeze-dried.

Comparative Example 1: Preparation of Abalone General Extract

Abalone general extract used as a control was obtained through the same hydrothermal extraction process without the fermentation process. That is, 10 times the amount of dry weight of distilled water was added and mixed well, and extracted by heating under reflux at 80 ℃ for 3 hours to separate the supernatant and the precipitate was extracted three times. Each extract was air-cooled and then filtered with a filter paper having a permeation size of 8 mu m. The filtered extract was concentrated under reduced pressure at 60 ° C. or lower and completely dried using a reduced pressure dryer to obtain a general abalone extract.

Comparative Example 2: Preparation of Abalone Yeast Fermented Extract

Abalone yeast fermented extract used as a control was mixed well by adding distilled water of 10 times the dry weight of abalone flesh and viscera powder, adjust the pH to 4.0 to 5.0 to 6.5 to 7.5, and pre-cultured yeast ( Bacillus subtilis ) Was added to a concentration of 10 ⁷ to 10 ⁸ CFU / mL. Yeast was incubated for 24 to 72 hours at 50-60 ℃ temperature. To terminate the fermentation, heat was applied at 80 ° C. for 10 minutes to induce a loss of function of the yeast. The precipitate was removed using a centrifuge and the supernatant was collected, or the filtered supernatant was concentrated under reduced pressure at 60 ° C. or lower and then lyophilized.

Experimental Example 1: Determination of the Components and Contents of the Abalone Fermented Extract

1-1: Determination of total phenol content and total flavonoid content

Total phenol content determination was performed as follows. 200 μl of 1N Folin Ciocalteau solution was added to 200 μl of sample, and 200 μl of 10% sodium carbonate solution was added thereto and left at room temperature for 10 minutes. The reaction solution was centrifuged at 150 g for 10 minutes and the supernatant was measured for absorbance at 540 nm using a microplate reader (Chromate, USA). At this time, the standard curve was prepared by diluting the concentration of galic acid to 0.1%, and the total phenol content of the sample was calculated from the calibration curve.

Total flavonoid content measurement was performed as follows.

100 μl of sample solution, 300 μl of ethanol, 20 μl of 10% aluminum nitrate solution, 20 μl of 1M potassium acetate solution, and 560 μl of water were added thereto, and the mixture was sufficiently stirred. After standing at room temperature for 40 minutes, the liquid layer was measured for absorbance at 405 nm using a microplate reader (manufactured by Chromate). The blank test was conducted in the same manner using an ethanol solution instead of a sample solution, and a blank was used in which water was used instead of an aluminum nitrate solution. The standard curve was prepared by diluting quercetin to 0.05% concentration, and calculated the total flavonoid content of the sample from the calibration curve, and the results are shown in Table 1 (total phenol content and total flavonoid content).

Name of sample Total phenol content
(Μg / mg) Total flavonoid content
(Μg / mg) Example 1 22.8 20.1 Comparative Example 1 15.3 5.9 Comparative Example 2 22.1 9.8

1-2: Chondroitin Sulfate Content

Chondroitin sulfate was measured according to the dietary supplement. In other words, 3 g of the sample was mixed with water to make 100 ml, and 4 ml of this solution was precisely taken up to 20 ml with water, followed by filtration using a room for quantitative analysis (Advantec, USA 5C). Separately, 5 ml of sodium borate sulfate solution was added to each of two colorimetric tubes, and after cooling sufficiently with ice water, 1 ml of the sample solution and the standard solution were carefully added on the reagent and cooled in ice water. 0.2 ml of the solving solution was added correctly, mixed, heated for 15 minutes in a water bath, and cooled to room temperature with ice water.The same operation was carried out using 1 ml of water for these liquids as a control solution, and a microplate reader (Chromate) Product) and the absorbance at 530 nm was measured, and the results are shown in the following Table 2 (content of chondroitin sulfate).

Name of sample Contains Chondroitin Sulfate (%) Example 1 41 Comparative Example 1 14 Comparative Example 2 40

Experimental Example 2: Antioxidant Activity

To measure the antioxidant activity of the abalone mushroom fermentation, 2.2-diphenyl-1-picryl hydrazyl; DPPH, 2.2-diphenyl-1-picryl hydrazyl, hereinafter referred to as “DPPH”) radical scavenging assay was used. 500 μM DPPH solution was dissolved in ethyl alcohol, filtered, and used immediately. The prepared DPPH solution and the sample solution were mixed at a ratio of 1: 1 and mixed vigorously. The mixture was allowed to stand at room temperature for 20 minutes. After absorbance was measured at 540 nm using a microplate reader (manufactured by Chromate), DPPH radical removal rate (%) was calculated through Equation 1 below. At this time, 1mM of ascorbic acid was used as a positive control to remove radicals, and the results are shown in Table 3 (antioxidative activity of the abalone fermentation extract).

[Equation 1]

DPPH radical scavenging (%) = ((A B)) / A * 100

Where A is the absorbance of the blank test solution and B is the absorbance of the test solution.

density(%)
Antioxidant capacity (%) Example 1 Comparative Example 2 0.0004 33 35 0.002 54 68 0.01 96 95

Experimental Example 3: Skin wrinkle improvement

3-1: Collagen Biosynthesis Evaluation

A procollagen assay was performed to measure the amount of collagen newly generated in fibroblasts. Fibroblasts, one of the cell lines involved in collagen production, were treated with 1% Antibiotic-Antimycotic (AA, manufactured by Welgene, South Korea) and 10% Fetal bovine serum (Welzen). Dulbecco's modified essential medium (DMEM; Dulbecco's modified essential medium, manufactured by Welsen) was incubated in a 37 ° C., 5% carbon dioxide incubator (CO ₂ incubator). Fibroblasts of 2 × 10 ⁵ concentration were inoculated into 6 well plates (6 well plates) and incubated for 24 hours, and then the medium was removed, and serum-free medium was treated and starvated for 24 hours. After 24 hours, serum-free medium was removed, washed with phosphate buffered saline (PBS), and irradiated with 12.5 mJ in ultraviolet rays at 312 nm wavelength. Thereafter, the samples were treated by concentration in phenol red Dulbecco's denatured essential medium (phenolred free DMEM) and further incubated for 48 hours. 50 μM of ascorbic acid was used as a positive control, UV (+) group was used as a negative control, and only the treated group was used as a control. After fermentation treatment, the medium was harvested after the incubation for 48 hours. The collected media was measured for collagen amount using a Procollagen Type-I C-Peptide (PIP) EIA kit (MK101 from Takara Bio Inc.). 100 μl of an antibody-peroxidase conjugate solution was added to each well, 20 μl of a sample or standard was added thereto, mixed well, and left at 37 ° C. for 3 hours. After the reaction, the contents were removed and washed four times with 400 μl of phosphate buffered saline. After removal, 100 μl of substrate solution was added and reacted at room temperature for 15 minutes. Then, 100 μl of stop solution was added and mixed gently. After measuring the absorbance at 450 nm using a microplate reader (manufactured by Chromate), a standard concentration curve was prepared to calculate the amount of collagen, and the results are shown in Table 4 (Collagen due to stimulation by ultraviolet rays of the abalone fermentation extract). Biosynthesis).

Test group Procollagen Concentration (µg / mL) 0.0004% 0.002% UV (+) 78 AS 25μM 101 Example 1 145 165 Comparative Example 1 112 120 Comparative Example 2 125 140

As shown in Table 4, Example 1, Comparative Example 1 and Comparative Example 2 (80 ℃, 3 hours) were compared together to look at the anti-wrinkle activity by the stimulation of the ultraviolet light of the fermented products. Compared with Comparative Example 1 and Comparative Example 2 was confirmed that the amount of newly generated collagen increased when treated with Example 1 according to the present invention.

3-2: MMP-1 Inhibition Evaluation

There are various kinds of enzymes that break down collagen, and the most known ones include MMP-1 that breaks down collagen type I; Matrix metalloproteinase I, collagenase) was performed to analyze the MMP-1. Fibroblasts, one of the cell lines involved in collagen production, were treated with Dulbecco's denatured essential medium (from Welzen) containing 1% antibiotic-antifungal (from Welzen) and 10% fetal bovine serum (from Welsen). Incubated in a 5% carbon dioxide incubator. Fibroblasts at 2 × 10 ⁵ concentrations were inoculated into 6 well plates and incubated for 24 hours, after which the medium was removed and the serum-free medium was treated and fasted for 24 hours. After 24 hours, the serum-free medium was removed, washed with phosphate buffered saline, and irradiated at 12.5 mJ in ultraviolet light with a UV 312 nm wavelength. Thereafter, the samples were treated by concentration in phenol red Dulbecco's denatured essential medium (phenolred free DMEM) and further incubated for 48 hours. 50 μM of ascorbic acid was used as a positive control, the UV (+) group was used as a negative control, and only the treated group was used as a control and compared with the results of the samples. After incubation for 48 hours after the extract treatment, the medium was collected. The collected media was measured for absorbance at 450 nm with an ELISA reader using an MMP-1 measurement kit (RPN 2610 from Amersham Bioscience, UK). A buffer was added to a single well to a 96 well plate coated with an anti-MMP1 antibody to be a control, and 100 μl of the sample and standard solution liberated in the medium were added to each well. . The reaction was carried out for 2 hours without shaking at room temperature. The wells were filled with a washing buffer, washed three times, and then completely drained. 100 μl peroxide conjugated solution was added and reacted for 2 hours without stirring at room temperature. The wells were filled with washing buffer and reacted with stirring for 30 minutes. After the reaction was stopped with 100 μl of 1M sulfuric acid (H ₂ SO ₄ ), the absorbance was measured at 450 nm using a microplate reader (manufactured by Chromate), and the results are shown in Table 5 below. Inhibition degree of MMP-1 activity by the stimulation by ultraviolet rays).

Test group MMP-1 (% of control) 0.0004% 0.002% UV (+) 100 AS 50μM 73 Example 1 99 84 Comparative Example 1 114 90 Comparative Example 2 98 82

As shown in Table 5, in order to examine the anti-wrinkle activity of the fermentation products by the stimulation by ultraviolet rays, the case treated with Example 1 was compared with the case treated with Comparative Example 1 and Comparative Example 2 (80 C, 3 hours). It was confirmed that the inhibition of MMP-1 activity was lower when treated with Example 1 than when treated with Comparative Example 1. This shows the results corresponding to the collagen biosynthesis of Table 4.

Experimental Example 4: Measurement of moisturizing power using a desiccator

5 ml of each of Example 1 and Comparative Example 1 were placed in a plate, and the total weight of dried calcium chloride (CaCl ₂ ) was used as a hygroscopic agent in a 35 ° C. desiccator. The weight was measured for a total of 144 hours. The moisturizing power was calculated according to the following Equation 2, and the experimental results of comparing 1,3-butylene glycol (BG), which is generally used as a moisturizing ingredient in cosmetic formulations, as a control, are shown in Table 6 Moisturizing power measurement using a micrometer).

&Quot; (2) "

Moisturizing Strength (%) = (1-△ S / S) * 100

Where S is the weight of the initial sample and ΔS is the weight of the final sample minus the weight of the final sample.

Time (hr) Moisture power (%) Control group
(BG) Comparative Example 1 Example 1 24 100 100 100 48 92 87 95 72 85 75 93 120 82 70 90 144 74 58 88

As shown in Table 6, compared to the Comparative Example 1 and 1,3-butylene glycol it can be seen that the moisturizing power of the abalone mushroom fermentation according to the present invention is significantly higher over time.

Formulation Example 1: Emulsion Base Preparation

An emulsion base including a cosmetic containing the abalone mushroom fermented product of Example 1 was prepared. The cosmetics used in this experiment is in the form of an emulsion cosmetic liquid, its composition is shown in Table 7 below. First, after adding the abalone mushroom fermented product (Example 1) and other contents recorded in Table 7, by weight, (a) the phase was heated to maintain at 90 ℃, purified water (30%) was heated to 90 ℃ Thereafter, the mixture was slowly added to the (a) phase to emulsify it homogeneously with a homomixer. Thereafter, the mixture was cooled to room temperature, and the remaining (b) phase purified water was added and stirred uniformly to prepare a cosmetic liquid. Hereinafter, "suitable amount" means a known amount so that it can be easily carried out according to the manufacturer's instructions or by those of ordinary skill in the art.

division ingredient weight% end Example 1 1.5 glycerin 3.00 Disodium iodide 0.02 Polyglyceryl-3-methylglucoside distearate 1.6 Cetearyl alcohol 0.5 Caprylic / capric triglyceride 6.8 Polyacrylamide & C13-14 Isoparaffin & Laureth-7 0.60 antiseptic Suitable amount I Purified water Remaining capacity (up to 100)

Experimental Example 5: Evaluation of Skin Moisturizing

5-1: Skin moisture increase effect

In order to measure the moisturizing effect of the skin of cosmetics containing the abalone mushroom fermentation according to the present invention as an active ingredient, it was prepared in Formulation Example 1 for 40 women aged 20 to 40 feel dry skin by a survey The applied cosmetics were applied twice a day to the face and then used for one month. The skin conductivity is measured using a moisture meter (courier Khazaka electronic GmbH, corneometer CM825, Germany) from constant temperature and humidity conditions (temperature 20-22 ° C, relative humidity 40%). The rate of increase in moisturization was evaluated by measuring the skin conductivity after 1 week, 2 weeks, and 4 weeks. In this case, a lipid mixture (ceramide: cholesterol: fatty acid = 2: 1: 1) was used instead of the extract as a positive control group, and the results are shown in Table 8 (skin moisture growth rate (%)). Indicated.

Test group 1 week Two weeks 4 weeks Comparative Example 1 42 53 65 Example 1 53 60 73 Positive control group 38 46 62

As shown in Table 8, it was confirmed that the skin moisture increase rate of the cosmetic composition comprising the abalone mushroom fermentation according to the present invention as an active ingredient is superior to the positive control group as well as Comparative Example 1.

5-2: Evaluation of Skin Dryness Improvement (Survey)

Subjective efficacy evaluation was conducted through a questionnaire survey of the test subjects of Experimental Example 5-1, and the results are shown in Table 9 (Skin dryness improvement evaluation). As a result, both the general abalone extract (Comparative Example 1) and the abalone mushroom fermented product (Example 1) obtained excellent results in the subjective evaluation of drying feeling of the subjects.

Test group Respondents Very good Good usually Inadequate Example 1 13 6 One 0 Comparative Example 1 9 7 4 0 Positive control group 5 5 9 One

Experimental Example 6: Measurement of Skin Elasticity Effect

In order to measure the test for skin elasticity effect of cosmetics containing the abalone mushroom fermentation product according to the present invention, 10 healthy men and women in their 40s and 40s. The cosmetics prepared in Formulation Example 1 were used, and the subjects were treated in a constant temperature and humidity chamber (temperature 20-22 ° C., relative humidity 40-60%) at baseline, 2 weeks, 4 weeks, and 6 weeks for 1 hour after cleansing. After stabilizing the skin, measurements were taken. The elasticity of the skin was measured by the cutometer SEM 474 (Curage Kazaka Electronics GmbH, Germany) and measured the area around the eyes. The numerical value was used, and the result is shown in Table 10 (skin elasticity measurement).

Test group Skin elasticity (%) Example 1 37 Comparative Example 1 12

As shown in Table 10, the cosmetics containing the abalone mushroom fermentation (Example 1) according to the present invention as an active ingredient is three times more skin elasticity than the abalone general extract (Comparative Example 1) I could confirm it.

Experimental Example 7: Skin Safety Test

In order to measure the skin safety test of cosmetics containing the abalone mushroom fermented product according to the present invention as an active ingredient, a conventional patch test was performed. The subjects were 15 healthy men and women in their 30s and 40s, and the cosmetics prepared in Experiment 5 were applied to the inner lower arm of the lower arm, and after 48 hours, the stylus was removed to observe the condition of the skin. The results are shown in Table 11 (skin safety measurement).

Test group Patch test (number of people) *** ** * Example 1 48 hours later 0 0 15 Comparative Example 1 48 hours later 0 One 14 ***: Very irritating
**: slight irritation
*: No stimulus (voice)

Experimental Example 8: Whitening Effect Test on Human Skin

The subjects were 10 adult males and females in their 30s and 40s, and 1.5 to 2 times the minimum erythema dose (Minimal Erythema Dose) of each subject. UV light was irradiated to a degree to induce skin blackening. After irradiation, 1% of each test substance (solvent 1,3-butylene glycol: ethanol = 7: 3), Arbutin 2% as a control, only a solvent (vehicle), one place without any change of state for 10 weeks Was observed. Skin color was measured on a weekly basis with a colorimeter (CR2002, Minolta, Japan). The difference in skin color (ΔL *) at the start and completion of application was calculated according to Equation 3 below, and the results are shown in Table 12 (whitening effect on skin). The whitening effect is determined by comparing ΔL * between the sample application site and the control site. When the value of ΔL * is about 2, the whitening of the deposited pigment is apparent, and when it is about 1.5 or more, the whitening effect may be determined. .

&Quot; (3) "

△ L * = L * value at the time of application completion-L * value at the start of application

Test group Melanin production inhibition rate (%) Example 1 1.92 ± 0.13 Comparative Example 1 1.16 ± 0.25 Solvent (Vehicle) 0.50 ± 0.15 Arbutin 1.56 ± 0.11

From the results of Table 12, it was confirmed that the abalone mushroom fermented product (Example 1) according to the present invention showed an excellent whitening effect than the arbutin, a positive control as well as the general abalone extract (Comparative Example 1).

Experimental Example 9: Elastin Activity Inhibition Test

According to the characteristics of the present invention, the effect of inhibiting the activity of elastase that inhibits the elasticity of the skin was tested. This test results in the development of yellow color when Elastase (Elastase, Sigma, USA) decomposes N-Succinyl Trialanine P-Nitroanilide (Sigma). The degree of inhibition is calculated by measuring the degree of color development with an absorbance meter. More specifically, first, the extract was dissolved in distilled water to prepare a sample by diluting to the required concentration. The buffer was prepared by adjusting the 0.26M Tris solution to pH 8.0. The substrate solution was prepared in 1 ml of buffer solution with 8.8 mM of elastase substrate N-succinyl trialanine P-nitroanilide. Elastase enzyme (Elastase) was made to a concentration of 10 ㎍ / ㎖ buffer. 25 μl of the sample, 25 μl of enzyme, and 75 μl of buffer were mixed from the prepared samples, and the whole reaction was performed at 25 ° C. for 10 minutes. After the pre-reaction, 125 µl of the substrate solution was added and reacted at 25 ° C. for 30 minutes, and then the absorbance was measured at 405 nm. As a blank test solution, a buffer solution was added instead of the sample, and the inhibition rate of the elastase activity was calculated by the following Equation 4. Inhibition of the elastin activity of the extract of the abalone Pleurotus eryngii mycelia as a control example 1, Comparative Example 1, Comparative Example 2 and the control, the results are shown in Table 13 below.

&Quot; (4) "

% Inhibition of elastase activity = ((A B)) / A * 100

Where A is the absorbance of the blank test solution and B is the absorbance of the test solution.

Test group Concentration (%) 0.05 0.1 Example 1 1.2 46.9 Mushroom Mycelium Extract 0.8 41.2 Comparative Example 2 -One -2.8 Comparative Example 1 -2.3 -3.6

From the results of Table 13, the abalone mushroom fermented product (Example 1) according to the present invention was confirmed to show an excellent elastase inhibitory effect than the extract of abalone mushroom mycelium as well as the general abalone extract (Comparative Example 1) It was.

Prescription example of cosmetic composition

The following is the formulation of the cosmetic composition as a formulation containing the abalone mushroom fermented product (Example 1) according to the present invention in each cosmetic formulation to remove active oxygen in the skin and have skin whitening, skin elasticity strengthening, whitening and wrinkle improvement effects. Examples are given, and the prescription is as follows.

Formulation Example 1 Preparation of Skin Lotion (Flexible Cosmetic)

Raw material name weight% Example 1 1.0 Butylene glycol 2.5 Oleyl alcohol 1.5 ethanol 5.0 Polysorbate 80 0.5 Polyethylene glycol 12 nonylphenyl ether 0.2 Carboxyl Vinyl Polymer 0.1 glycerin 3.5 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Remaining capacity (up to 100)

Formulation Example 2: Preparation of Milk Lotion (Nutrition Makeover)

Raw material name weight% Example 1 1.5 Squalane 4.5 Wax 3.8 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Polysorbate 60 1.5 Butylene glycol 3.2 Capric triglyceride 5.0 Propylene glycol 3.0 glycerin 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Remaining capacity (up to 100)

Formulation Example 3: Essence Preparation

Raw material name weight% Example 1 4.0 Butylene glycol 3.0 glycerin 3.0 Allantoin 0.7 Panthenol 0.2 Sodium Ethylenediaminetetraacetate (EDTA-2Na) 0.05 ethanol 4.0 Triethanolamine 1.5 Squalane 2.0 Wax 2.0 Polysorbate 60 3.0 Carboxyl vinyl polymer 1.0 Sorbitan sesquioleate 2.8 Preservative, coloring, incense Suitable amount Purified water Remaining capacity (up to 100)

The present invention can be used in the field of fermentation industry and industry using abalone.

Although the present invention has been described in detail only with respect to the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the technical scope of the present invention, and such modifications and modifications are within the scope of the appended claims.

Claims (14)

Abalone mushroom fermentation product obtained by fermenting abalone with mushroom mycelium. The method of claim 1,
Abalone mushroom fermented product, characterized in that the abalone includes both abalone meat and abalone intestine. The method of claim 1,
The mushroom mycelia are mycelium of Ganoderma lucidum , mycelium of Phellinus linteus , mycelium of Hericium erinaceum , mycelium of abalone mushroom ( Pleurotus cystidiosus ) and a mixture of two or more of them Abalone mushroom fermentation, characterized in that is selected from. (1) an inoculation step of inoculating abalone with mushroom mycelium; And
(2) a fermentation step of fermenting abalone inoculated with mushroom mycelium;
Method for producing an abalone mushroom fermentation, characterized in that comprises a. 5. The method of claim 4,
The abalone mushroom in the inoculation step of the abalone mushroom fermented product, characterized in that it comprises both abalone meat and internal organs. 5. The method of claim 4,
The mushroom mycelium at the inoculation step is mycelium of Ganoderma lucidum , mycelium of Phellinus linteus , mycelium of Hericium erinaceum , mycelium of Pleurotus cystidiosus and two of them Method for producing an abalone mushroom fermentation, characterized in that selected from the group consisting of the above mixture. 5. The method of claim 4,
A method of producing abalone mushroom fermentation, characterized in that the pretreatment step of quenching and lyophilizing the abalone is further performed before the inoculation step. The method of claim 7, wherein
Abalone mushroom fermentation, characterized in that the culture step of preparing a culture solution for cultivating mycelium by grinding the dried abalone obtained in the pretreatment step between the pretreatment step and the inoculation step is further carried out. Method of producing water. 5. The method of claim 4,
Method for producing abalone mushroom fermentation, characterized in that the further step of maturing the fermentation broth obtained in the fermentation step after the fermentation step is carried out. The method of claim 9,
The aging step is a method of producing abalone mushroom fermentation, characterized in that carried out by storing the fermentation broth at 3 to 10 ℃ for 1 to 3 days. 5. The method of claim 4,
Concentrating the abalone mushroom fermented product, characterized in that the after-treatment step to obtain a freeze-dried fermented product by concentrating and lyophilizing the fermentation broth obtained after the fermentation step to a water content of 10%. The method of claim 9,
The method of producing abalone mushroom fermented product, characterized in that the post-treatment step of obtaining a freeze-dried fermented product by concentrating and freeze-drying the fermentation broth obtained after the aging step to have a water content of 10%. 0.001 to 80% by weight of a lyophilized fermentation product comprising the abalone mushroom fermentation according to any one of claims 1 to 3, obtained by lyophilization as an active ingredient based on the total weight of the cosmetic composition. Moisturizing, elasticity, whitening, cosmetic composition for improving skin wrinkles, characterized in that it comprises in an amount within the range. A lyophilized fermented product obtained by lyophilization as an active ingredient, the abalone mushroom fermentation product obtained according to any one of claims 4 to 12, based on the total weight of the cosmetic composition. Moisturizing, elasticity, whitening, skin wrinkle improvement cosmetic composition, characterized in that it comprises in an amount within the range of%.