JP2008201773A - External preparation for skin - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an external preparation for skin, which can be suitably used for restoring the decrease of extracellular matrix components caused by ultraviolet rays, active oxygen, aging and the like, for improving functions of cutaneous tissue, and is useful for improving cutaneous troubles such as wrinkles, chapped skin and the like and for recovery of the skin injury. <P>SOLUTION: The external preparation for skin comprises aqueous components of defatted lees of fruits and/or seeds of Camellia japonica belonging to the genus Camellia of the family Theaceae. The external preparation for skin has fibroblast proliferation-promoting effect, collagen and/or hyaluronic acid production potentiation effect and skin antiaging effect. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

The present invention relates to an external preparation for skin comprising as an active ingredient an aqueous component of camellia (Camellia japonica) berries and / or seed defatted straw belonging to the genus Camellia.

The skin consists of epidermis, dermis and subcutaneous tissue due to its constitution. The epidermis is in contact with the outside world and is composed of the stratum corneum, granule layer, spiny layer, and basal layer, and the keratinocytes (keratinocytes) produced in the basal layer repeat division and undergo horny cells through granule cells. Covers the skin surface, and old keratinocytes become plaque and peel off. The sum of the time required for the keratinocytes to reach the stratum corneum from the basal layer (about 14 days) and the period for protecting the skin surface as keratinocytes (about 14 days) is referred to as turnover as skin metabolism. Unlike the epidermis, the dermis tissue has few cells and is mainly composed of extracellular components such as proteins such as collagen and elastin, and mucopolysaccharides such as hyaluronic acid and chondroitin sulfate. It plays roles such as tissue support, water retention in the interstitial space, skin lubricity and flexibility retention, protection of skin tissue from external factors such as ultraviolet rays, dry environment, mechanical irritation and damage, microbial infection, etc. . These extracellular components are produced by fibroblasts (Non-Patent Document 1).

The extracellular matrix components produced by fibroblasts are routinely denatured and decomposed under the influence of active oxygen and microorganisms or by irradiation with ultraviolet rays, resulting in skin wrinkles, spots, freckles, roughness, rough skin, etc. Causes skin problems. In addition, it is known that various functions of a living body decrease with aging, the tissue ages, and the content of extracellular components such as hyaluronic acid in the skin tissue also decreases (Non-patent Document 2). When the content of hyaluronic acid or collagen in the skin tissue decreases, dry skin, rough skin, decreased elasticity and flexibility, decreased tension and gloss, increased skin wrinkles, sagging and dullness, and skin aging symptoms Wake up. Therefore, in order to maintain healthy skin, it is necessary to replenish the extracellular matrix component. For this purpose, it is desirable to activate fibroblasts, which are the component-producing cells in the dermal tissue.

Attempts to search for an activator of dermal fibroblasts have been studied in the past. Hibiscus extract (Patent Document 1), L-ascorbic acid and its derivatives (Patent Document 2), almond, dandelion, assembly, hop Extract (Patent Document 3), collagen hydrolyzed tripeptide (Patent Document 4), rosemary extract containing Genkwanin (Patent Document 5), α-D-glucopyranosylglycerol (Patent Document 6), A polypeptide having a specific amino acid sequence (Patent Document 7) and the like have been proposed.

These ingredients and extracts have been disclosed, for example, to be used in cosmetics and foods and drinks, but when used for cosmetics, coexistence stability with other raw materials and ingredients used in combination is disclosed. There are not a few things that are lacking, and there are some that are difficult in terms of percutaneous absorption, so the practical effects on the skin troubles are not fully exhibited, and the physiological function of the skin tissue was not essentially improved. . In addition, when used for food and drink, there is a risk of deterioration or degradation in the gastrointestinal tract, and there are few that can exhibit effectiveness in practical use. In addition, depending on the raw materials and components used in combination, the actual product may affect the color tone, flavor, physical properties, etc. of the practical product, and was not always satisfactory in terms of stability, usage, cost, etc. . Therefore, an effective material capable of improving the skin trouble has been demanded.

The following is known about the camellia described later. That is, camellia has a history of being used as an ornamental horticultural plant since ancient times, and fats and oils collected from seeds are used as fuel oil, hair styling, high-grade edible oil, etc., and xylem is ashed to brew sake. The actual defatted rice bran has been used as a fertilizer for agricultural crops. The defatted cocoons contain saponins and tannins, and there are also proposals for processing them into insecticides (Patent Document 8), agricultural and horticultural nematodes control agents (Patent Document 9), and the like. However, there are no examples of using ingredients contained in camellia nuts and / or seed defatted cocoons for skin improvement.

Japanese Patent Laid-Open No. 9-295928 Japanese National Patent Publication No. 10-509735 Japanese Patent Laid-Open No. 10-36279 JP 2002-255847 A JP 2004-137217 A JP 2004-331578 A JP 2006-265221 A Japanese Patent No. 170071 JP-A-9-30916 Michihiro Hattori, “Science of Skin Care”, pp. 6-14 and pp. 15-83, Hankabo Co., Ltd., published on February 25, 1997 Maria O. Longas et al., “Evidence for structural change in dermatological sulfate and hyaluronic acid with aging” (Netherlands), 1987, Carbohydr. Res. 159, 127-136

In view of the current situation, the present inventors have recovered the reduction of the extracellular matrix component content in the skin tissue caused by a decrease in metabolic function caused by ultraviolet rays, active oxygen, aging, etc. An object of the present invention is to develop a safe and stable material for improving skin and preventing and / or improving the above-mentioned troubles of the skin, and to provide an external preparation for skin using the same.

In order to solve the above problems, the present inventors have conducted extensive studies on the metabolic mechanism of the extracellular matrix components in the skin tissue and materials that promote its production. Surprisingly, camellia is extremely effective, and it activates dermal fibroblasts, promotes their proliferation, enhances the production of the extracellular matrix components by the cells, and significantly improves skin aging symptoms. The present inventors have found that a component to be obtained is contained, and that it can be effectively used for an external preparation for skin, and have completed the present invention.

That is, according to the present invention, there is provided an external preparation for skin comprising as an active ingredient an aqueous component of camellia (Camellia japonica) berries and / or seed defatted straw belonging to the genus Camellia.

The external preparation for skin is at least selected from the group consisting of an external preparation for skin fibroblast growth promotion, an external preparation for skin collagen and / or hyaluronic acid production enhancement, and an external preparation for skin anti-aging. One type is desirable. Here, it is preferable that the aging of the skin includes at least one symptom selected from the group consisting of wrinkles, spots, dullness, buckwheat, sagging, roughness and rough skin. Further, in the above-mentioned external preparation for skin of the present invention, the aqueous component which is an active ingredient is extracted from water and / or lower alcohol with defatted camellia and / or seeds of Camellia japonica belonging to the genus Camellia family Camellia. An extract is desirable.

According to the present invention, there is further applied to and / or contacting the skin with an aqueous component of camellia (Camellia japonica) berries and / or seed defatted straw belonging to the camellia family Camellia. Methods are provided for promoting cell growth, enhancing skin collagen and / or hyaluronic acid production, and / or preventing skin aging. Here, the aqueous component is preferably an extract obtained by extracting defatted pods of Camellia japonica and / or seeds belonging to the genus Camellia belonging to Camelliaaceae with water and / or a lower alcohol.

The aqueous component extracted from camellia (Camellia japonica) berries and / or seed defatted lees according to the present invention is excellent in quality stability, promotes the proliferation of fibroblasts in the skin, It enhances the production of hyaluronic acid and promotes skin turnover to improve skin troubles such as skin wrinkles, spots, dullness, buckwheat, sagging, roughness, and rough skin. In addition, it promotes regeneration of damaged skin and contributes to maintaining skin health. Such an effect is remarkably exhibited by applying and / or contacting the aqueous component to the skin. Therefore, according to the present invention, there is provided a skin external preparation comprising the aqueous component as an active ingredient, and in particular, a skin external preparation for promoting the proliferation of skin fibroblasts, skin collagen and / or hyaluronic acid. It can be effectively used as a skin external preparation for enhancing production or as a skin external preparation for preventing skin aging.

The present invention is described in detail below. First, the external preparation for skin of the present invention has an action of promoting the proliferation of fibroblasts present in living tissue, particularly the dermal tissue of the skin, and belongs to the genus Camellia of Theaceae family. It is characterized by containing as an active ingredient an aqueous component of (Camellia japonica) berries and / or seed defatted cocoons.

As the plant belonging to the camellia genus, generally, the camellia camellia belonging to the camellia section, such as Camellia japonica, the tea belonging to the tea section (C. sinensis), the sasanqua belonging to the sasanqua section, C. sasanqua, etc. (C. granthamiana) and the like, and the genus Sasanqua (C. salicifola) and the like, and the C. lutchuensis and the like belonging to the camellia section are known in the present invention. Use. Examples of this include C. japonica var. Japonica, C. japonica subsp. Rusticana, C. japonica var. Macrocarpa, C. japonich. Hong Konggenesis, C. reticulata, C. salenensis, Pitaldi var. partardii, and C. partidi var. Yamabe camellia (same kind as Ayaba camellia), wild tea flower (same kind as Ayers camellia), Kushimatsubaki can be mentioned (apple camellia and the like) and the like. What is necessary is just to utilize suitably these camellia which are growing naturally in the Japanese archipelago, the Korean peninsula, the Shandong peninsula of China, etc.

In the present invention, the camellia nuts and / or seeds are subjected to a compression treatment, an extraction treatment using a hydrophobic organic solvent such as hexane or heptane, or a liquefied gas such as liquefied carbon dioxide or liquefied propane. The defatted soot, which is a residue extracted from, is used as an essential raw material. Here, the camellia seeds and / or seeds may be either early-ripening seeds or mature seeds, and these seeds may be used. From the viewpoint of defatted koji and the yield of the active ingredient, mature seeds or seeds thereof may be used. desirable. In the present invention, it is convenient and preferable to use seeds obtained from mature fruits dried for about 1-2 weeks in the sun.

The aqueous component of the defatted lees can be produced by any method, but it is preferable to extract it with water and / or lower alcohol. The lower alcohol has a tendency to extract the oily substance in the defatted soot as the carbon number increases, so that a lower carbon number is desirable, such as methanol, ethanol, normal propanol, isopropanol, normal butanol, isobutanol, etc. It can be illustrated. When using a lower alcohol having a large carbon number, it is preferable to increase the water content in order to suppress the extraction of oily components in the defatted soot. For example, the water content in the case of propanol is about 20 wt% to about 50 wt%, and the water content in the case of ptanol is about 40 wt% to about 70 wt%. Desirable extraction solvents are water, methanol and ethanol, and water-containing alcohols thereof (water content: 0 to 100% by weight).

In order to extract the defatted lees, the extraction solvent is added in an amount of about 1 to 30 times by weight with respect to 1 part by weight of the defatted lees, at about normal temperature or 1 to 5 atm. After stirring and mixing for 10 minutes to about 3 hours as necessary, the mixture is cooled to room temperature and filtered, and the filtrate is concentrated and dried by an appropriate means such as drying under reduced pressure, spray drying or freeze drying. The dried product may be appropriately pulverized. In this way, a light yellow to yellow solid which is an aqueous component of the defatted soot according to the present invention can be obtained. The extraction method is preferably performed by repeatedly extracting the extraction residue once extracted, or under a pressure of 1 to 3 atmospheres at about 100 ° C. to about 130 ° C. This increases the yield of the aqueous component according to the present invention. This aqueous component includes saponins, tannins, kaempferol, glycosides thereof and the like.

In the external preparation for skin of the present invention, the aqueous component as an active ingredient is in the form of a solid, paste or liquid, and this may be used as it is as an external preparation for skin. It can also be prepared as a composition by using a known method in combination with known additives to be used. Here, the known additive may be any additive that is used in cosmetics, toiletry products, etc. and does not violate the gist of the present invention. For example, excipients, binders, disintegrants, lubricants, and the like may be used. Various additive substances such as a bulking agent, a wetting agent, a fluidizing agent, a preservative, a surfactant, a stabilizer, a diluent, a solubilizer, a disinfectant, a preservative, a colorant, and a fragrance can be used. In addition, it is possible to use materials that are known to promote fibroblast proliferation, enhance collagen and / or hyaluronic acid production, or prevent aging.

Among the above-mentioned known additives, examples of excipients include cellulose and derivatives thereof, starch, modified starch, dextrin, resistant digestive dextrin, sugar alcohols such as lactose, mannitol, sorbitol, dicalcium phosphate, mica, talc, etc. Is mentioned.

Binders and disintegrators include cellulose derivatives such as crystalline cellulose, methylcellulose, ethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, hydroxypropylmethylcellulose acetate succinate, carmellose sodium, starch derived from wheat, rice, corn, potato, etc. Pregelatinized starch, partially pregelatinized starch, modified starch such as hydroxypropyl starch, dextrin, pullulan, polyvinylpyrrolidone, aminoalkyl methacrylate copolymer, methacrylic acid copolymer, polyvinyl acetal diethylaminoacetate, polyvinyl alcohol, gum arabic, agar, gelatin, white Examples include shellac, tragacanth, and macro goal.

As a lubricant, for example, starch derived from wheat, rice, corn, potato, stearic acid, calcium stearate, magnesium stearate, hydrous silicon dioxide, light anhydrous silicic acid, synthetic aluminum silicate, dried aluminum hydroxide gel, talc , Magnesium aluminate metasilicate, calcium hydrogen phosphate, anhydrous calcium hydrogen phosphate, sucrose fatty acid ester, waxes, hydrogenated vegetable oil, polyethylene glycol and the like.

Wetting agents, moisturizing agents, and emollients include squalane, squalene, lecithin, lysolecithin, cholesterol, sphingolipid, serine, glutamine, sorbitol, mannitol, glycerin, pyrrolidone carboxylic acid, 1,3-butylene glycol, propylene glycol, dipropylene Glycol, lactic acid and its salt, hyaluronic acid, sodium hyaluronate, collagen, water-soluble collagen, hydrolyzed elastin, alginic acid and its salt, mucopolysaccharide, polyethylene glycol, polyaspartate, water-soluble chitin, water-soluble chitosan, glucosamine And its derivatives, N-acetyl-D-glucosamine, cholesteryl long-chain acyl glutamate, cholesteryl hydroxystearate, hydrogenated castor oil, 12-hydroxystearic acid Ricinoleic acid, stearic acid, rosin acids, lanolin, lanolin fatty acid cholesteryl ester, isopropyl myristate, cetyl palmitate, isononyl isononanoate, silicone oils (cyclomethicone, dimethicone, cyclomethicone, etc.), can be exemplified milk whey, or the like.

Examples of the fluidizing agent include fine silicon dioxide, hydrous silicon dioxide, light anhydrous silicic acid, dry aluminum hydroxide gel, synthetic aluminum silicate, magnesium silicate and the like.

Antioxidants include ascorbic acid, tocotrienol, dl-α-tocopherol, α-tocopherol, γ-tocopherol, δ-tocopherol and other tocopherols, tocopherol acetate, isopropyl citrate, disodium calcium ethylenediaminetetraacetate, dibutylhydroxytoluene Phytic acid, caffeic acid, catechin, gallic acid, propyl gallate, erythorbic acid and its sodium salt, dilauryl thiodipropionate, L-cysteine hydrochloride and the like.

Examples of preservatives and preservatives include benzoic acid, sorbic acid, paraoxybenzoic acid, methyl paraoxybenzoate, propyl paraoxybenzoate, isopropyl paraoxybenzoate, propionic acid, sodium sulfite, chlorobutanol and the like.

Examples of surfactants include glycerophospholipids such as soybean lecithin, egg yolk lecithin, lysolecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, polyglycerin fatty acids such as sucrose fatty acid ester, glycerin fatty acid ester, diglycerin fatty acid ester Sorbitan fatty acid esters such as esters, sorbitan monostearate, sorbitan sesquioleate, polyoxyethylene higher alcohol ethers, polyoxypropylene higher alcohol ethers, polyoxyethylene fatty acid esters, polyoxypropylene fatty acid esters, polysorbate 80, polyoxyethylene sorbitan Nonionic surfactants such as fatty acid esters, polyoxyethylene hydrogenated castor oil, benzil chloride Cationic surfactants such as conium, stearyltrimethylammonium chloride, dicetyldimethylammonium chloride, and behenyltrimethylammonium chloride; amphoteric surface activities such as 2-cocoyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine and betaine amidoacetate Agent, higher alcohol sulfate, higher alcohol ether sulfate, long chain fatty acid alkali metal salt, long chain fatty acid alkaline earth metal salt, long chain fatty acid basic amino acid salt, N-long chain acyl amino acid, N-long chain acyl amino acid There are anionic surfactants such as salts.

Examples of the stabilizer include paraoxybenzoates such as methylparaben and propylparaben, alcohols such as chlorobutanol, benzyl alcohol, and phenylethyl alcohol, benzalkonium chloride, acetic anhydride, sorbic acid, and the like.

Diluents, solubilizers, solubilizers include purified water, ethanol, lauryl sulfate triethanolamine, ethylene glycol, diethylene glycol, propylene glycol, dipropylene glycol, glycerin, olive oil, castor oil, silicone oil, liquid paraffin, diclodextrin, etc. is there.

Examples of isotonic agents include sodium chloride, potassium chloride, glycerin, boric acid and the like.

Examples of pH adjusters include lactic acid, succinic acid, fumaric acid, citric acid, malic acid, tartaric acid, gluconic acid, and sodium or potassium salts thereof, glucono delta lactone, adipic acid, sodium acetate, arginine, triethanolamine. Sodium hydroxide, potassium hydroxide, phosphoric acid, sodium dihydrogen phosphate, tripotassium hydrogen phosphate and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid derivatives such as paraaminobenzoic acid and octylparadimethylaminobenzoate, benzophenone derivatives such as 2-hydroxy-4-methoxybenzophenone and dihydroxydimethoxybenzophenone, ethyl paramethoxycinnamate, and octyl paramethoxycinnamate. Methoxycinnamic acid derivatives such as octyl salicylate, salicylic acid derivatives such as homomenthyl salicylate, α-dehydroamino acid derivatives such as N-benzoyl-O-methyl-α-dehydrotyrosine-2-ethylhexyl ester, 4- (3,4-dimethoxy) Benzalhydantoin derivatives such as phenyl) methylene-2,5-dioxo-1-imidazolidinepropionic acid 2-ethylhexyl ester, urocanic acid, ethyl urocanate, 4-ter-butyl -4'-methoxydibenzoylmethane, 2- (2'-hydroxy-5'-methylphenyl) benzotriazole, and the like.

Examples of the disinfectant include hinokitiol, triclosan, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc pyrithione and the like.

Examples of colorants include zinc oxide, titanium oxide, iron oxide, silica, talc, mica, copper chlorophyll, water-soluble anato, β-carotene, riboflavin and its butyrate, gardenia yellow, blue No. 1, red No. 202, edible Examples include Red No. 2, No. 105, Edible Yellow No. 4, Edible Green No. 3, Edible Blue No. 2, and the like.

In addition, various fragrances and plant essences can be used as necessary, and as fats and oils, vegetable oils such as avocado oil, olive oil and jojoba oil, fatty acids such as oleic acid and isostearic acid, petrolatum, lanolin, ceresin, microcrystalline wax, Carnauba wax, candelilla wax, coconut oil fatty acid, lauric acid, hardened beef tallow fatty acid and other fatty acids, cetyl 2-ethylhexanoate, isopropyl myristate, 2-octyldodecyl myristate, neopentyl glycol di-2-ethylhexanoate, Ester oil such as glycerol tri-2-ethylhexanoate, oleic acid-2-octyldodecyl, glycerol triisostearate, 2-ethylhexanoic acid diglyceride, ester oil such as long chain acyl glutamic acid octyldodecyl ester, Siloxane, methyl hydrogen polysiloxane, methylphenyl polysiloxane, silicone oils such as octamethylcyclotetrasiloxane, liquid paraffin, can be used squalene, etc. in appropriate liquid hydrocarbon oils squalane.

In addition to the materials described in the above-mentioned patent document, the fibroblast proliferation promoting action is known as chlorella, aloe vera, rice, jujube, moon peach, mango ginger, no grape, spinach fern, lotus germ, sesame, chili pepper, toki, Dokudami, Haskup fruit, Kusunohagashi, algae (kaurelpa, racemosa), dried products or extracts of plants and algae such as strawberry, barley, catechins, imino group-containing peptides, α-lipoic acid and its salts, esters, amides, etc. Examples thereof include derivatives, dihydrolipoic acid and derivatives thereof, chitin hydrolysates, N-acetyl-D-glucosamine and oligomers thereof.

Known materials for enhancing collagen and / or hyaluronic acid production include grapes, aloe vera, itadori, dokudami, saffron, maca, Dutch pepper, licorice, litchi, cuckoo, germinated soybeans, beech, birch, chlorella and yeast Extract, genquanine, tocotrienol, isoflavone, diethylenetriaminepentaacetic acid, N-acetylglucosamine, placenta extract, soluble eggshell membranes, sericin and its hydrolyzate, adenosine phosphate ester, angiogenin and its degradation product, interleukin-1, Examples include phytol, hydrogenated retinoid, N-methyl-L-serine, and the like.

In addition to the aforementioned fibroblast growth-promoting substances and collagen and / or hyaluronic acid production-enhancing substances, retinoic acid, glycolic acid, α-hydroxy acid, hibiscus, cucurbitaceae (pumpkin, loofah) Examples include seed extracts, vitamins A, C, D and E, N-acylproline and the like. In addition, this invention is not limited at all by these each illustration.

The form of the external preparation for skin of the present invention is not particularly limited, and it is a preparation for lotions, emulsions, gels, creams, ointments and the like for all those applied to the skin, hair and scalp. For example, lotion, emulsion, cream, foundation, pack, essence, lipstick, face wash, shampoo, rinse, hair tonic, hair treatment and the like can be mentioned. In addition, quasi-drugs such as ointments, poultices, bath preparations, cleaning agents and aerosols may be included.

In order to produce the external preparation for skin of the present invention, the above-mentioned known additives are appropriately selected, and a predetermined amount of the aqueous component according to the present invention is added thereto, followed by processing by a usual production method. Here, the amount of the aqueous component according to the present invention is about 0.01 wt% to about 90 wt%, more desirably about 0.1 wt% to about 70 wt%. If the amount is less than about 0.01% by weight, the external preparation for skin of the present invention may not exhibit the desired effect, and if it exceeds about 90% by weight, it may be difficult to process a normal dosage form as an external preparation for skin. The external preparation for skin of the present invention can be used by a method of applying to or contacting the skin, hair, or scalp in order to prevent and / or ameliorate the above-mentioned troubles of the skin.

As described above, the external preparation for skin of the present invention contains an aqueous component of camellia (Camellia japonica) berries and / or seed defatted cocoons belonging to the genus Camellia as an active ingredient. Preferably, it is a skin external preparation for promoting the proliferation of skin fibroblasts, a skin external preparation for enhancing skin collagen and / or hyaluronic acid production, and / or a skin external preparation for preventing skin aging. It is an external preparation for skin applicable to at least one of these uses.

Types and parts of camellia applied to the above-mentioned skin external preparation for promoting fibroblast proliferation, defatted lees, extraction methods and conditions thereof, aqueous components, blending amounts of aqueous components, combined raw materials, forms as external skin preparations, usage methods Etc. are the same as in the case of the aforementioned external preparation for skin. Here, the fibroblast is more desirably a fibroblast in the dermal tissue. In addition, in this external preparation for skin, it is desirable to use the aqueous component according to the present invention in combination with a material having a known fibroblast proliferation promoting action.

Types and parts of camellia applied to the above-mentioned collagen and / or hyaluronic acid production enhancing skin external preparation, defatted lees, extraction methods and conditions, aqueous components, blending amounts of aqueous components, combined raw materials, forms as a skin external preparation The method of use and the like are the same as in the case of the aforementioned external preparation for skin. Here, the production of collagen and / or hyaluronic acid is more desirably the production of collagen and / or hyaluronic acid by fibroblasts in the dermal tissue. In this external preparation for skin, it is desirable to use the aqueous component according to the present invention in combination with a material having a known action for enhancing production of collagen and / or hyaluronic acid.

The above-mentioned skin external preparation for preventing skin aging has the effect of improving the above-mentioned skin troubles and damages, and the types of camellia applied to this, defatted lees, extraction methods and conditions, aqueous components, aqueous The compounding amount of the components, the combination raw material, the form as a skin external preparation, the method of use and the like are the same as in the case of the aforementioned external preparation for skin. Here, it is more desirable that the symptoms of skin aging include at least one symptom selected from the group consisting of skin wrinkles, spots, dullness, buckwheat, sagging, roughness, and rough skin. In addition, in this skin external preparation, it is desirable to use together the aqueous component which concerns on this invention, and the raw material with an anti-aging effect | action known.

Next, although an Example is given and this invention is demonstrated in detail, this invention is not limited by this. In each example, all the percentages, parts and ratios are based on weight.

Production Example 1
After coarsely crushing dried seeds of C. japonica var. Japonica from Goto, Nagasaki Prefecture, steaming and then pressing to obtain a press knead separated from the compressed oil, followed by adding normal hexane to the press koji and extracting by a conventional method After processing, the extract was separated and the extract was collected. The extracted soot was washed with normal hexane to remove oil, and defatted soot was collected. 400 mL of water was added to 100 g of this defatted soot, heated to 85 ° C. under normal pressure and stirred for 1 hour as appropriate, then cooled to room temperature and filtered to separate the filtrate. 200 mL of water was again added to the filtration residue and heated in the same manner. After cooling, the filtrate was collected by filtration. Both filtrates were combined, concentrated under reduced pressure, lyophilized and pulverized to obtain 15.8 g of a powder (referred to as sample 1) containing an aqueous component according to the present invention. This powder was hydrolyzed and analyzed by HPLC. As a result, it contained 16.1% sapogenin which is an aglycon of saponin and 2.5% kaempferol which is a kind of flavonol.

Production Example 2
The dried seeds of Yakushima apple camellia (C. japonica var. Macrocarpa) were defatted by the method described in Production Example 1 and defatted cocoons were collected. 400 mL of water was added to 100 g of the defatted soot, and the mixture was heated at 120 ° C. for 25 minutes under a pressure of 2 atm, then cooled to room temperature and filtered to separate the filtrate. 200 mL of water was again added to the filtration residue and heated in the same manner. After cooling, the filtrate was collected by filtration. Both filtrates were combined, concentrated under reduced pressure, lyophilized and pulverized to obtain 16.4 g of a powder containing the aqueous component according to the present invention (referred to as sample 2). The powder was hydrolyzed in the same manner as in Production Example 1 and analyzed by HPLC. As a result, the sapogenin content was 14.8% and the kaempferol content was 2.4%.

Production Example 3
300 g of water-containing ethanol (water content 35%) was added to 100 g of the defatted lees obtained by the method described in Production Example 1, and the mixture was heated to reflux at 80 ° C. for 1 hour, cooled to room temperature, and filtered to separate the filtrate. To this filtration residue, 200 mL of water-containing ethanol (water content 35%) was added again and heated in the same manner. After cooling, the filtrate was collected by filtration. Both filtrates were combined, concentrated under reduced pressure, freeze-dried and pulverized to obtain 11.3 g of powder (referred to as sample 3) containing an aqueous component according to the present invention. The powder was hydrolyzed in the same manner as in Production Example 1 and analyzed by HPLC. As a result, the sapogenin content was 12.8% and the kaempferol content was 2.1%.

Production Example 4
Ethanol (purity 99.5%) 300 mL was added to 100 g of the defatted lees obtained by the method described in Production Example 2, and the mixture was heated to reflux at 80 ° C. for 1 hour, cooled to room temperature, and filtered to separate the filtrate. 200 mL of ethanol (purity 99.5%) was again added to the filtration residue and heated in the same manner. After cooling, the filtrate was collected by filtration. Both filtrates were combined, concentrated under reduced pressure, freeze-dried and pulverized to obtain 4.5 g of a powder (referred to as sample 4) containing an aqueous component according to the present invention. As a result of hydrolysis and HPLC analysis of the powder in the same manner as in Production Example 1, the sapogenin content was 14.0% and the kaempferol content was 2.4%.

Production Example 5
In Production Example 1, except that the dried seeds were replaced with immature seeds (whole seeds including the whole seeds), the same treatment was performed to obtain a defatted koji, and then 16.7 g of a powder containing an aqueous component (referred to as sample 5). Got. The powder was hydrolyzed in the same manner as in Production Example 1 and analyzed by HPLC. As a result, the sapogenin content was 15.0% and the kaempferol content was 2.2%.

Test Example 1: Skin Fibroblast Growth Promoting Effect The effect of the powder (Sample 1 to Sample 5) containing the aqueous component according to the present invention on the proliferation of skin fibroblasts was examined by the following method. That is, normal human adult dermal fibroblasts (manufactured by Kurabo Industries Co., Ltd., NHDF (AD), hereinafter simply referred to as cells) were used with 10% fetal bovine serum (Daiichi Chemicals Co., Ltd.) using a petri dish (φ10 cm). 2 × 10 ⁵ cells were seeded on D-MEM medium (manufactured) supplemented (Sigma, low glucose) and cultured for 4 days until it became sub-confluent (approximately 80% density). Next, the medium is removed, and the cells are washed twice with 5 mL of PBS and further with 5 mL of 0.02% EDTA solution, and then the cells are recovered using 5 mL of 0.25% trypsin solution (manufactured by Nacalai Tesque). After centrifugation (4 ° C., 1,000 rpm, 5 minutes), the supernatant was removed, and the cells were washed twice with PBS to obtain cells. These cells were repeatedly cultured under the above conditions and subcultured.

Using a 96-well cell culture plate (0.32 cm ² , manufactured by Asahi Techno Glass Co., Ltd.), the subcultured cells were transformed into a human serum fibroblast proliferation low serum medium (Kurabo Co., Ltd., LSGS added 106S medium). : 1 × 10 ⁴ cells / well in a dermal fibroblast basal medium (106S) supplemented with 500 mL of low serum growth additive (LSGS) and cultured for 24 hours. Subsequently, the medium was removed, and the culture was further continued for 48 hours in the low serum medium for human skin fibroblast proliferation to which each sample was added so that the final concentration was 5, 10 or 20 μg / mL. Thereafter, 25 μL of MTT solution (PBS in which thiazolyl blue tetrazolium bromide (manufactured by Sigma, reagent) was dissolved) at a concentration of 5 mg / mL was added and cultured for 1 hour. After completely removing the medium by decantation, a formazan solution (25% (v / v) 0.45 M acetate buffer (pH 4.5), 25% (v / v) N, N-dimethylformamide, 10% ( w / v) Contains sodium n-dodecyl sulfate, pH 4.5) was added and stirred. After standing overnight at room temperature, the absorbance at 590 nm was measured to evaluate the degree of cell proliferation. In the above method, the D-MEM medium and the low serum medium for human dermal fibroblast proliferation were supplemented with penicillin (final concentration 100 IU / mL) and streptomycin (final concentration 0.1 mg / mL). All were performed in a CO ₂ incubator (37 ° C., 5% CO ₂ -enhanced gas phase).

The results are shown in Table 1. In the table, the numerical values are shown as relative values when the value of the control test (when no sample is added) carried out at the same time is taken as 100. From the data in Table 1, the aqueous component of the defatted lees according to the present invention has the effect of promoting the growth of human dermal fibroblasts, and this effect can be achieved by heating the defatted lees under normal pressure or under pressure. It was confirmed that when water was extracted, it became remarkable when ethanol was extracted under heating.

Test Example 2: Hyaluronic Acid Production Enhancement Action The effect of the powder containing the aqueous component according to the present invention (Sample 1 to Sample 5) on hyaluronic acid production enhancement by skin fibroblasts was examined by the following method. That is, dermal fibroblasts subcultured by the method described in Test Example 1 were used for proliferating human dermal fibroblasts using a 96-well cell culture plate (0.32 cm ² , manufactured by Asahi Techno Glass Co., Ltd.). Low serum culture medium (Kurabo Co., Ltd., LSGS-added 106S medium: skin fibroblast basal medium (106S) added with 10 mL of low serum growth additive (LSGS)) seeded at 1 × 10 ⁴ cells / well. Cultured for 1 day. Subsequently, each sample was added to the LSGS-added 106S medium so that the final concentration was 5, 10 or 20 μg / mL, and the culture was further continued for 48 hours. All of this culture solution was collected and used as a test solution for the determination of hyaluronic acid by the following ELISA test.

The hyaluronic acid content in the cell culture solution was analyzed by an ELISA test method using a commercially available hyaluronic acid measurement kit (manufactured by Seikagaku Corporation). That is, the cell culture solution was diluted 16-fold with the buffer solution of the kit, and 50 μL was added to the hyaluronic acid solid-layered microplate. Next, 50 μL of a biotin-labeled HABP solution was added thereto, followed by mixing for 1 minute, and a primary reaction was performed at 37 ° C. for 1 hour. Further, after removing the solution in the well and washing the plate, 100 μL of HRP-labeled streptavidin solution was added, and a secondary reaction was performed at 37 ° C. for 1 hour. Thereafter, the plate was washed by removing the solution in the well, 100 μL of the enzyme substrate solution was added, and the enzyme reaction was performed at room temperature for 30 minutes under light shielding. After adding 100 μL of the reaction stop solution and mixing, the absorbance was measured with a plate reader (measurement wavelength: 490 nm, control wavelength: 620 nm). The hyaluronic acid content in the test solution was determined from a calibration curve using a hyaluronic acid standard product.

The results are shown in Table 2. In the table, the numerical values are shown as relative values when the value of the control test (when no sample is added) carried out at the same time is taken as 100. From the data of Table 2, the aqueous component of the defatted lees according to the present invention has the effect of enhancing hyaluronic acid production by human dermal fibroblasts, and this action is obtained by extracting the defatted lees with water under heating and pressure. It was confirmed that it would be remarkable if

Test Example 3: Preventing skin aging (part 1)
A 5-week-old female hairless mouse (purchased from Japan SLC Co., Ltd.) was allowed to freely take basic feed (manufactured by Clea Japan Co., Ltd., CE-2) and drinking water for 1 week, then 1 group 3 As animals, UV irradiation non-irradiation group (control group), UV irradiation + purified water application group (positive control group), UV irradiation + sample 1 (1% aqueous solution) application group, UV irradiation + sample 2 (1% aqueous solution) application group The group was divided into an ultraviolet irradiation group, an ultraviolet irradiation + sample 3 (1% aqueous solution) application group, an ultraviolet irradiation + sample 4 (1% aqueous solution) application group, and an ultraviolet irradiation + sample 5 (1% aqueous solution) application group. Ultraviolet irradiation was performed by irradiating the back of the mouse with UV-B at an intensity of 30 mJ / cm ² for 5 minutes once, 5 times every week for 12 weeks. Each sample solution was applied once a day at a frequency of 0.1 mL to a specific position (diameter: about 2.5 cm) on the skin during the ultraviolet irradiation period. The test was conducted under these conditions, and the degree of wrinkles and rough skin formed on the mouse skin of each group was visually observed and evaluated.

As a result, wrinkle formation and rough skin were not observed in the control group that was not irradiated with ultraviolet rays, but clear wrinkle formation and rough skin were generated in the positive control group that was irradiated with ultraviolet rays. On the other hand, in each of the groups where each sample was applied while being irradiated with ultraviolet rays, wrinkle formation and rough skin due to ultraviolet irradiation were almost halved. From this, it was confirmed that the said aqueous component which concerns on this invention has the skin aging prevention effect | action which can reduce the skin trouble by ultraviolet irradiation.

Test Example 4: Preventing skin aging (part 2)
An oil phase component consisting of squalane: 10 parts, stearic acid: 5 parts, cetanol: 3 parts and stearic acid monoglyceride: 3 parts was heated and dissolved at 80 ° C., while glycerin: 15 parts, methyl paraoxybenzoate: 0. 1 part, carboxyvinyl polymer (1% aqueous solution): 10 parts, polyglycerol (average polymerization degree 4) monooleate: 3 parts, arginine (20% aqueous solution): 15 parts, sample 2: 1 part and purified water: 34 The aqueous phase component consisting of 9 parts was dissolved by heating at 80 ° C. The oil phase component was added to the aqueous phase component with stirring, and the mixture was uniformly emulsified with a homogenizer to prepare a test cream. Further, in this cream, a comparative cream (adjusted with purified water) not containing Sample 2 was prepared. 10 volunteer women (22 to 54 years old, average age: 31.0 years old) apply the test cream to the left hand and the comparative cream to the right hand twice a day for 2 weeks. Was conducted a questionnaire survey (three-step evaluation).

As a result, (1) About the dryness of the skin, roughness, and rough skin, the comparative cream is strong: 5 people, normal: 4 people, weak: 1 person, and the test cream is strong: 1 person, normal: 3 Name, weak: 6 people. (2) About the elasticity and flexibility of the skin, the comparison cream has many: 2 people, normal: 3 people, few: 5 people, and the test cream has many: 6 people, normal: 3 people, less: 1 It was a name. (3) Regarding skin tension and gloss, strong (increase) in the comparative cream: 2 people, normal: 3 people, weak (less): 5 people, strong in the test cream (increase): 7 people, Normal: 2 people, weak (less): 1 person. (4) Skin wrinkles, sagging, and dullness are more common in the comparative cream: 7 people, normal: 2 people, less: 1 person, more in the test cream: 1 person, normal: 3 people, less: 6 It was a name. From this, by continuously applying the aqueous component according to the present invention to the skin, dry skin and rough skin are reduced, the elasticity and flexibility of the skin are increased, the skin tension and gloss are increased, and wrinkles and gloss are increased. It was confirmed that sagging decreased.

Prototype examples 1-7
Various cosmetics were made on a trial basis using the following formulations (all values are% by weight).
(1) Lotion (a) Sorbit 2
(B) 1,3-butylene glycol 4
(C) Polyethylene glycol 1000 2
(D) Ethanol 10
(E) Sample 1 1
(F) Preservative (Methylparaben) Appropriate amount (g) Purified water The remaining amount (a) to (f) is added to (g) heated to 80 ° C., stirred and dissolved, then cooled to room temperature and filled into a container did.

(2) Latex (a) Squalane 4
(B) Vaseline 1
(C) Stearyl alcohol 0.5
(D) Sorbitan monostearate 1.5
(E) Polyoxyethylene (20) sorbitan monooleate 3
(F) 1,3-butylene glycol 5
(G) Sample 2 0.1
(H) Tea catechin 0.1
(I) Purified water The remaining amounts (a) to (d) were heated and dissolved at 80 ° C. to obtain an oil phase component, and (e) to (i) were heated and dissolved at 80 ° C. to obtain an aqueous phase component. At the same temperature, the oil phase component was added to the aqueous phase component with stirring, and the mixture was uniformly emulsified with a homogenizer, cooled to room temperature, degassed, and filled into a container. This emulsion can be used as a skin external preparation for promoting the proliferation of dermal fibroblasts.

(3) Cream (a) Squalane 20
(B) Beeswax 5
(C) Jojoba oil 5
(D) Sorbitan monostearate 1
(E) Glycerol monooleate 3
(F) Polyoxyethylene (20) sorbitan monostearate 3
(G) Glycerin 5
(H) Sample 3 5
(I) N-acetylglucosamine 2
(J) Purified water The remaining amounts (a) to (e) were heated and dissolved at 80 ° C. to obtain an oil phase component, and (f) to (j) were heated and dissolved at 80 ° C. to obtain an aqueous phase component. At the same temperature, the oil phase component was added to the aqueous phase component with stirring, and the mixture was uniformly emulsified with a homogenizer, cooled to room temperature, degassed, and filled into a container. This cream can be used as a skin external preparation for enhancing skin collagen and / or hyaluronic acid production, or as a skin external preparation for enhancing skin collagen and / or hyaluronic acid production and preventing aging.

(4) Body soap (a) Potassium laurate 13
(B) Potassium myristate 5
(C) Propylene glycol 7
(D) Sample 4 10
(E) Hibiscus flower extract 3
(F) pH adjuster (citric acid) appropriate amount (g) preservative (methyl paraben) appropriate amount (h) purified water The remaining amount (a) and (b) were dissolved in advance by heating at 80 ° C. (c) to (h) The mixture was added to the solution with stirring and mixed uniformly with a homogenizer, then cooled to room temperature, degassed and filled into a container. This body soap can be used as a skin external preparation for preventing skin aging.

(5) Shampoo (a) Lauric acid diethanolamide 2
(B) Lauryl sulfate triethanolamine 5
(C) Polyoxyethylene lauryl ether sodium sulfate 12
(D) 1,3-butylene glycol 4
(E) Edetate disodium 0.1
(F) Sample 2 0.05
(G) Preservative and perfume appropriate amount (h) Purified water The remaining amount (b) to (h) is heated and dissolved to 70 ° C., (a) is added, emulsified with a homomixer, cooled and deaerated. Filled into a container.

(6) Hair tonic (a) Ethyl oleate 1
(B) Ethanol 45
(C) Polyoxyethylene (40) hydrogenated castor oil 2
(D) Sample 1 15
(E) Purified water The remaining amounts (c) to (e) were dissolved by heating to 60 ° C., the mixture of (a) and (b) heated to the same temperature was added, solubilized by a homomixer, cooled, Degassed and filled into containers.

(7) Powder bath agent (a) Sodium bicarbonate 50
(B) Anhydrous sodium sulfate 35
(C) Borax 3
(D) Sample 3 7
(E) Chamomile extraction powder 4
(F) Colorant and fragrance Each appropriate amount (a) to (f) was mixed with a powder mixer and filled into a container.

Aqueous components obtained from camellia seeds and / or seed defatted have skin fibroblast growth promoting action, collagen and / or hyaluronic acid production enhancing action, anti-aging action, etc. By contacting, the original physiological function of the skin can be recovered, and it can be used as an external preparation for skin useful for improving skin troubles and early recovery of skin damage.

Claims (4)

  An external preparation for skin comprising, as an active ingredient, an aqueous component of camellia (Camellia japonica) berries and / or seed defatted lees belonging to the genus Camellia.   For promoting fibroblast proliferation of skin, enhancing collagen and / or hyaluronic acid production, comprising as an active ingredient an aqueous component of camellia (Camellia japonica) fruit and / or seed defatted koji belonging to the genus Camellia And / or a skin external preparation for preventing aging. The skin external preparation according to claim 1 or 2, wherein the aqueous component is an extract obtained by treating the defatted koji with water and / or a lower alcohol.   To promote the growth of fibroblasts on the skin, characterized by applying and / or contacting the skin with an aqueous component of camellia japonica (Camellia japonica) fruit and / or seed defatted cocoon A method for enhancing the production of collagen and / or hyaluronic acid in the skin and / or for preventing skin aging.