KR101685579B1

KR101685579B1 - Method for manufacturing skin-whitener composition containing Thuja orientalis and skin-whitener composition prepared therefrom

Info

Publication number: KR101685579B1
Application number: KR1020150113925A
Authority: KR
Inventors: 김수경; 서동구; 차영주
Original assignee: 김수경; 서동구; 차영주
Priority date: 2015-08-12
Filing date: 2015-08-12
Publication date: 2016-12-12

Abstract

The present invention relates to a method for preparing a skin whitening cosmetic composition containing Thuja orientalis and using natural-derived materials and a skin whitening cosmetic composition prepared thereby, the method comprising the steps of: (A) mixing: Thuja orientalis; at least one selected from the group consisting of green tea leaves, Perilla ocymoides, Huttuynia cordata, and Lycium chinense; brown rice; nuruk; grain syrup; useful microorganisms; mineral concentrated water; deep sea water; and loess water, followed by first fermentation; (B) injecting air at the same temperature as in the first fermentation, followed by second fermentation; (C) mixing: Thuja orientalis; at least two selected from the group consisting of Angelica gigas, ginseng, Korean asarum, Huttuynia cordata, Ganoderma lucidum roots, Saururus chinensis, and Perilla ocymoides; at least two selected from the group consisting of Hypsizygus marmoreus, Lingzhi mushroom, Scutellaria baicalensis, salicomia herbacea, green tea leaves, Citrus peels, Artemisia annua, Artemisia campestris; at least two selected from the group consisting of Scrophularia buergeriana, Lycium chinense, and frankincense; at least two selected from the group consisting of kelp, Hizikia fusiformis, and Agarum cribrosum; Cryptotympana pustulata; mineral concentrated water; and deep ocean water, followed by hot-water extraction; (D) filtering a fermentation material fermented in step (A) to separate a fermentation liquid and residues; (E) hot-water extracting the residues separated in step (D); and (F) mixing the fermentation liquid separated in step (D), the extract extracted in step (D), and the extract extracted in step (C), so the skin whitening cosmetic composition suppresses the generation of melanin pigment and thus can be favorably used in skin whitening, and has an excellent moisturization effect.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin whitening cosmetic composition,

The present invention relates to a method for producing a skin whitening cosmetic composition using a naturally occurring substance including a hyphae, which is effective for skin whitening and has excellent moisturizing effect by inhibiting the production of melanin pigment, and a skin whitening cosmetic composition prepared thereby.

It is almost impossible for skin to regenerate by restructuring the skin structure in a vigorous state when the age is stopped, and it is almost impossible to regenerate the skin at the time when the growth period is stopped. However, It is possible that it can be turned into a more elastic and smooth skin.

In Korea, an aging society is rapidly emerging as medical and public health benefits are improved. In fact, it is predicted that an aging society with one in four people aged 65 and over will arrive.

Accordingly, the quality of life is being improved, and the desire for the maintenance of youth is also increasing. Therefore, many researches are being conducted in various fields in order to delay and prevent the appearance which is the first appearance of the skin. Particularly, there is an increasing demand for cosmetics that emphasize functionalities such as whitening and wrinkle improvement in the field of cosmetics manufacturing.

In this regard, anti-wrinkle agents known so far include a moisturizing agent type that improves wrinkles by restoring normalized keratinization to dry skin by moisturizing dry skin, enhancing collagen biosynthesis, A substance having an antioxidative effect, a substance inhibiting transdermal water evaporation, and the like.

Of these, hyaluronic acid or sodium chondroitin sulfate and lactic acid are examples of moisturizing agents having anti-wrinkle effect, and retinoic acid widely used in pharmaceuticals is known to have excellent anti-wrinkle effect. However, retinoic acid has been reported to have side effects such as redness of the skin and sensitivity to ultraviolet rays, and it has a problem that it is effective only when it is continuously used for 6 months or more.

Skin, on the other hand, has several important functions, the most typical of which is the ability to inhibit melanin production to protect the body from ultraviolet light from sunlight. Melanin is produced in melanocytes, a kind of skin cells, and has a natural screening ability that is distributed on the surface of the skin to block ultraviolet rays harmful to the human body. Tyrosinase, which is synthesized at the melanocyte base, is an enzyme. Tyrosinase produces tyrosine (Dopaquinone) through dopa (DOPA) using an amino acid called tyrosine as a substrate. Melanin, which is a black pigment, is produced by a condensation reaction.

(A), and arbutin (A-4-9315), and extracts of some of them have been shown to inhibit tyrosinase activity And it is used as a whitening cosmetic. However, its use is limited due to its poor stability in a prescription system, coloring by decomposition, generation of odor, efficacy at a living body level, unclear effect and safety. The inhibitory effect of tyrosinase has been demonstrated, but its effect is lower in experiments similar to actual biomolecular levels. Therefore, an inhibitory effect by melanoma cell culture similar to a living body level is required.

In addition, hydroquinone is prescribed as a carcinogenic substance and its use is prohibited. Koji acid and ascorbic acid are very unstable substances, and browning occurs when a cosmetic containing a small amount is stored at room temperature for several weeks. Arbutin also causes cytotoxicity and safety issues are pointed out.

On the other hand, natural products are not only excellent in safety, but also have various beneficial ingredients, so that a cosmetic composition having excellent skin whitening and moisturizing properties by using a naturally derived substance is required.

Korea Patent Publication No. 2010-0007654 Korean Patent No. 0915711 Korean Patent Publication No. 2012-0033377

It is an object of the present invention to provide a method for manufacturing a skin whitening cosmetic composition using a natural-derived material containing a hyphae which is useful for whitening skin by suppressing the production of melanin pigment and having excellent moisturizing effect.

Another object of the present invention is to provide a skin whitening cosmetic composition produced by the above-described method.

In order to accomplish the above object, the present invention provides a method for manufacturing a skin whitening cosmetic composition using a natural-derived material comprising a papaya leaf, comprising the steps of: (A) preparing a skin whitening cosmetic composition comprising a papaya leaf, Angelica keiskei koidz., Green tea leaf, Fermenting the mixture of microorganism and mineral concentrated water, which are EM solution, and then adding the deep sea water and yellow clay water to the first fermentation;

(B) injecting air under the same temperature as that of the primary fermentation to perform secondary fermentation;

(C) Peppermint, Ginseng, Ginseng, Seeds, Seeds, Zygomycetes, Saururus chinensis, Zygomycota, Zelgite strand mushrooms, Ganoderma, Golden, Green tea, Green tea leaves, Extracting the seaweed, the seaweed, the mineral water, the deep seawater, and hot water;

(D) separating the fermented product fermented in step (B) into a fermentation broth and a residue;

(E) hot water extraction of the residue separated in step (D); And

(F) mixing the fermentation broth separated in step (D), the extract extracted in step (E), and the extract extracted in step (C).

50 to 600 parts by weight of the group consisting of Angelica keiskei, green tea leaf, true lobe leaf, 5 to 10 parts by weight of brown rice; 5 to 10 parts by weight of yeast; 100 to 300 parts by weight of crude oil; 100 to 300 parts by weight of useful microorganisms; 20 to 100 parts by weight of mineral concentrated water; 1500 to 2000 parts by weight of deep ocean water; And 1,500 to 2,000 parts by weight of yellow clay.

In the step (A), the primary fermentation may be performed at 20 to 50 ° C for 12 to 20 days.

In the step (B), the secondary fermentation may be performed for 12 to 20 days.

100 to 700 parts by weight of the group consisting of Angelica gigas Nakai, Ginseng, Seeds, Seeds, Zygomycetes, Saururus chinensis, 100 to 800 parts by weight of a group consisting of zelkova mushroom, Ganoderma lucidum, golden, green tea, green tea leaves, dermis, purple wormwood and rhizome; 50 to 250 parts by weight of the group consisting of ginseng, gugija and oriental; 50 to 200 parts by weight of a group consisting of kelp, tar and sea anchovy; 5 to 20 parts by weight; 20 to 50 parts by weight of mineral concentrated water; And deep sea water at 1000 to 20,000 parts by weight.

In the step (C), the hot-water extract may be obtained by extracting the mixture at 70 to 90 ° C for 100 to 130 hours.

In the above-mentioned steps (A) and (C), the mineral-concentrated water may have a hardness of 230,000 to 290,000 mg / L by concentrating the deep ocean water.

In the step (E), the hot-water extract may be a mixture of the residue and water at a weight ratio of 1: 1 to 3 and extracted at 70 to 90 ° C for 10 to 30 hours.

In step (F), the fermentation broth separated in step (D), the extract extracted in step (E), and the extract extracted in step (C) are mixed at a weight ratio of 1: 0.2-0.8: 0.5-3.0 .

In addition, the skin whitening cosmetic composition of the present invention for achieving the above-mentioned other objects can be manufactured according to the above-mentioned manufacturing method.

The skin whitening cosmetic composition using the naturally-occurring substance including the papillae of the present invention can effectively inhibit the production of melanin pigment by inhibiting the synthesis of tyrosinase involved in melanin pigment formation. Accordingly, the skin whitening cosmetic composition of the present invention has an excellent skin whitening effect through inhibition of the production of melanin pigment.

In addition, the skin whitening cosmetic composition of the present invention is excellent in moisturizing effect and excellent in skin penetration ability, and therefore, it is suitable for use in mist or ampule in various formulations.

The present invention relates to a method for producing a skin whitening cosmetic composition using a natural-derived material containing a hyphae which is useful for whitening skin by inhibiting the production of melanin pigment and having excellent moisturizing effect, and a skin whitening cosmetic composition prepared thereby.

Hereinafter, the present invention will be described in detail.

A method for preparing a skin whitening cosmetic composition using a natural-derived material comprising the lateral side of the present invention comprises the steps of: (A) mixing first side yeast and other natural materials to ferment; (B) injecting air under the same temperature as that of the primary fermentation to perform secondary fermentation; (C) mixing the leaves and other natural substances to extract hot water; (D) separating the fermented product fermented in step (B) into a fermentation broth and a residue; (E) hot water extraction of the residue separated in step (D); And (F) mixing the fermentation broth separated in step (D), the extract extracted in step (E), and the extract extracted in step (C).

In the step (A), At least one selected from the group consisting of green leaf, green leaf, Brown rice; yeast; Cho Chung; Useful microorganisms; After mixing the mineral concentrated water, deep ocean water; And yellow clinging factor are added to the first fermentation.

The hyphae refers to the branches and leaves of Thuja orientalis L. (Thuja orientalis L.), which acts on the lungs and meridians to lower fever. It is good for dry cough and bronchitis and has a hemostatic effect. , Nosebleed, anemia and uterine bleeding are also effective.

In the present invention, the papillae are obtained by mixing the branches and leaves of the papillae at a weight ratio of 1: 1-2. When the content of the leaf is less than the lower limit based on the branch, skin whitening and moisturizing effect can not be expected. If the content is above the upper limit, toxicity may be caused.

When at least one selected from the group consisting of Angelica keiskei L., green tea leaf, Japanese lobster, Rhododendron japonica and Gugija is fermented together with the leaves, the skin whitening effect is improved, . At least one selected from the group consisting of Angelica keiskei L., green tea leaf, Liliaceae, Rhodophyta and Rhizoma is used in an amount of 50 to 600 parts by weight based on 100 parts by weight of the leaves. : 1. When the content of at least one selected from the group consisting of Angelica keiskei, Green tea leaf, Zygomycorrhizae, Rhizoctoniaceae, and Gujilus is lower than the lower limit, excellent whitening effect can not be obtained, and skin trouble may occur when the content exceeds the upper limit.

The brown rice is preferably brown rice, which is blended with the leaves to enhance not only skin whitening but also moisturizing effect during fermentation. The brown rice is blended at 5 to 10 parts by weight, preferably 6 to 7 parts by weight, based on 100 parts by weight of the leaves. When the content of brown rice is less than the above lower limit, excellent whitening and moisturizing effect can not be obtained, and when the content is above the upper limit, further excellent effects can not be obtained and resources are wasted.

The yeast is a traditional fermentation agent of Korea and commonly used yeast is mixed with useful microorganisms to enhance skin whitening and moisturizing effect as well as skin penetration. If none of the yeast and useful microorganisms are used, the skin whitening and moisturizing effect may not be improved but may be lowered, and the skin penetration may not be improved.

Nuruk is mixed in 5 to 10 parts by weight, preferably 6 to 7 parts by weight, based on 100 parts by weight of the leaves. If the content of the yeast is below the lower limit, the fermentation may not be performed. If the content of the yeast exceeds the upper limit, the yeast is used more than necessary and resources are wasted.

The above-mentioned starch is used for fermentation, and specific examples thereof include cornstarch, bellflower, cornstarch and black garlic, preferably cornstarch.

The content of tonic acid is 100 to 300 parts by weight, preferably 150 to 200 parts by weight based on 100 parts by weight of the leaves. Fermentation may not be carried out when the content of oranges is less than the above lower limit, and in the case of exceeding the upper limit, waste more than necessary is used and resources are wasted.

The beneficial microorganism (EM) is mixed with koji and fermented to improve skin whitening and moisturizing effect as well as skin penetration.

The content of the useful microorganism is 100 to 300 parts by weight, preferably 100 to 150 parts by weight based on 100 parts by weight of the leaves. If the content of useful microorganisms is less than the lower limit, skin penetration may be lowered. If the content is above the upper limit, more useful microorganisms than necessary are used and resources are wasted.

The mineral-enriched water is obtained by concentrating deep-sea water having a hardness of 300 mg / L to a hardness of 230,000 to 290,000 mg / L. The hardness is an index indicating the content of calcium carbonate in the water, which corresponds to the sum of the contents of calcium ions and magnesium ions in water. The higher the hardness, the larger the amount of minerals contained. When the hardness of the mineral-concentrated water is less than the lower limit, minerals such as calcium and magnesium can not be supplied to the skin. If the hardness of the mineral-concentrated water is above the upper limit, skin troubles may occur.

The content of the mineral-concentrated water is 20 to 100 parts by weight, preferably 30 to 50 parts by weight, based on 100 parts by weight of the leaves. When the content of mineral-concentrated water is less than the lower limit value, excellent skin whitening and moisturizing effect can not be expected. If the content is above the upper limit value, skin trouble may occur.

In the present invention, it is necessary to first mix the mineral-concentrated water with high hardness and the natural-derived materials to coat or infiltrate a large amount of concentrated minerals on the surface of the natural-derived materials and then ferment them under deep sea water and yellow clay. Minerals can improve their efficacy by fermentation. At this time, when the deep sea water and the yellow clay are added together with mineral concentrated water later, the whitening and moisturizing effect may be lowered.

The content of the deep sea water is 1500 to 2000 parts by weight, preferably 1600 to 1700 parts by weight, based on 100 parts by weight of the leaves. If the content of the deep sea water is out of the above range, it may interfere with fermentation.

The above-mentioned yellow clay is a supernatant after standing for 20 to 25 hours in a mixture of yellow soil and water, and contains various minerals such as potassium, sodium and iron, which are contained in mineral concentrated water and deep seawater, .

The content of the yellow clay larvae is 1500 to 2000 parts by weight, preferably 1600 to 1700 parts by weight based on 100 parts by weight of the leaves. When the content of the yellow clay is less than the lower limit, various minerals can not be supplied to the skin. If the content is above the upper limit, the yellow clay is used more than necessary and resources are wasted.

The mixture mixed in the step (A) is firstly fermented at 20 to 50 ° C, preferably 25 to 30 ° C for 12 to 20 days, preferably 13 to 14 days. During the first fermentation, the fermentation vessel is turned over once a day to allow the fermentation to be performed well. When the fermentation temperature and time are out of the above range, fermentation may not be performed well, and in particular, if the fermentation temperature is higher than the upper limit value, skin trouble may be caused.

Next, in the step (B), air is injected under the same temperature as that of the primary fermentation for secondary fermentation.

Air is injected for 12 to 20 days, preferably 13 to 14 days at the same temperature as the primary fermentation, thereby performing secondary fermentation while air bubbles are formed in the fermentation product.

When fermentation is carried out without air injection, secondary fermentation is not smoothly performed and, in some cases, toxicity may be induced.

In the present invention, step (C) is performed separately from steps (A) and (B).

On the other hand, in step (C), At least one member selected from the group consisting of Angelica gigas, Ginseng, Sechin, Rhizoctonia sp. One or more selected from the group consisting of zelkova mushroom, Ganoderma lucidum, golden, green tea, green tea leaf, dermis, blue mugwort and mugwort; At least one selected from the group consisting of ginseng, gugija and frankincense; At least one selected from the group consisting of kelp, kotatsu, and horseradish; Retirement; Mineral concentrated water; And deep sea water are mixed and subjected to hot water extraction.

In the same manner as in step (A), the leaves are mixed with the leaves and leaves of the leaves in a weight ratio of 1: 1-2.

In addition, in order to improve the moisture and skin permeability significantly, it is preferable to use at least one selected from the group consisting of Angelica gigas, Ginseng, Sechin, Horseshoe chinensis, Are used together.

The content of at least one member selected from the group consisting of Angelica ginseng, Ginseng, Sezin, Hwasungcho, Geocachin, Saururus chinensis and Japanese leaf is 100 to 700 parts by weight based on 100 parts by weight of the leaves. If the content of at least one selected from the group consisting of Angelica ginseng, Panax ginseng, Sezin, Persimmon griseus, Zygomyceta, Saururus chinensis and Liliaceae is lower than the lower limit value, moisturizing and skin penetration may not be improved. May cause toxicity.

In order to further improve the skin whitening and moisturizing properties, the fermented product prepared in step (B) may be further mixed with a selected one selected from the group consisting of Zelkomania mushroom, Ganoderma, golden, green tea, green tea leaf, dermis, Or more, and preferably the above eight species are used together.

The content of at least one selected from the group consisting of Zelkova mushroom, Ganoderma lucidum, golden, green tea, green tea leaves, dermis, blue mugwort and mugwort is 100 to 800 parts by weight based on 100 parts by weight of the leaves. When the content of at least one selected from the group consisting of zelkova mushroom, Ganoderma lucidum, golden, green tea leaf, dandelion, blue mugwort and mugwort is less than the lower limit value, excellent skin whitening and moisturizing effect can not be expected, The skin penetration ability may be lowered.

Also, one or more selected from the group consisting of ginseng, gugija and frankincense are preferably used together with the fermentation product prepared in the step (B), preferably the above three kinds, in order to enhance the penetration into the skin.

The content of at least one selected from the group consisting of ginseng, gujie, and oriental is 50 to 250 parts by weight based on 100 parts by weight of the leaves. If the content of at least one selected from the group consisting of ginseng, gingko and oriental is lower than the lower limit, the skin penetration may not be improved. If the content is higher than the upper limit, the skin penetration may be lowered.

In order to reduce the toxicity and improve the moisturizing effect, at least one selected from the group consisting of sea tangle, red sea bream and sea bream is preferably used together with the above three species.

The content of at least one selected from the group consisting of kelp, kotatsu, and aquaculture is 50 to 200 parts by weight based on 100 parts by weight of the leaves. When the content of at least one selected from the group consisting of kelp, tortoiseshell, and perforated seaweed is less than the lower limit, excellent moisturizing effect can not be expected, and if it exceeds the upper limit, toxicity can be caused.

In addition, the cicada shell is a naked defect when the cicada is transformed, thereby lowering the heat of the skin and improving the moisturizing effect. When the content is less than the lower limit value, excellent moisturizing effect can not be expected. When the content is above the upper limit value, more waste is used and resources are wasted.

The content of the mineral-enriched water is 20 to 50 parts by weight, preferably 25 to 30 parts by weight, based on 100 parts by weight of the leaves. When the content of the mineral-concentrated water is less than the lower limit, excellent skin whitening and moisturizing effect can not be expected. If the content is above the upper limit, the moisturizing effect may be lowered.

The deep seawater is preferably used in an amount of 1000 to 20,000 parts by weight based on 100 parts by weight of the leaves.

The hot-water extract in step (C) is obtained by extracting the mixture at 70 to 90 ° C. for 100 to 130 hours, preferably 120 to 130 hours. If the extraction temperature and time are out of the above range, the active substances may not be extracted.

Next, in step (D), the secondary fermentation product fermented in the step (B) is filtered and separated into a fermentation broth and a residue.

The secondary fermented product is separated by filtration into a fermentation broth and a residue.

Next, in the step (E), the residue separated in the step (D) is extracted with hot water.

The residue separated in the step (D) and water are mixed at a weight ratio of 1: 1 to 3 and extracted with hot water at 70 to 90 ° C for 10 to 30 hours, preferably 15 to 20 hours to obtain an extract.

When the extraction temperature and the extraction time are out of the above range, the effective substance contained in the residue can not be extracted.

Next, in step (F), the fermentation broth separated in step (D), the residue extract extracted in step (E), and the extract extracted in step (C) are mixed.

The fermentation broth separated in step (D), the residue extract extracted in step (E), and the extract extracted in step (C) are mixed at a weight ratio of 1: 0.2-0.8: 0.5-3.0, 0.7: 1.0-1.5. If the content of the residue extract extracted at step (E) and the extract extracted at step (C) is lower than the lower limit value based on the fermentation broth separated in step (D), skin whitening and moisturizing effects can not be improved, If it exceeds the upper limit value, it may be toxic and skin trouble may occur.

Skin whitening and moisturizing effects are not improved if either of the fermentation broth separated in step (D), the residue extract extracted in step (E), and the extract extracted in step (C) is omitted.

The naturally-occurring materials used in the present invention should be prepared according to the manufacturing method of the present invention, so that complementary pharmacological effects can be exerted and excellent whitening effect and moisturizing power as well as excellent skin permeability can be obtained.

The skin whitening cosmetic composition of the present invention may contain, in addition to the skin whitening cosmetic composition prepared according to the above-described production method, other components generally added to cosmetics, if necessary. Examples of such ingredients include a moisturizing agent, An antioxidant, a pH adjuster, an alcohol, a pigment, a flavor, a blood circulation accelerator, a cold agent, a limiting agent, and purified water can be cited as the surfactant, surfactant, organic and inorganic pigment, organic powder, ultraviolet absorber, preservative,

The skin whitening cosmetic composition may be prepared in the form of an emulsified formulation and a solubilized formulation commonly used in the art, and examples thereof include a mist, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, Cream, nutritional cream, moisturizing cream, hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder, . In particular, the skin whitening cosmetic composition of the present invention is preferable for a mist or an ampule since it has excellent skin penetration ability.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

Manufacturing example 1. Preparation of the compound of step (A)

83 parts by weight of green tea leaves, 83 parts by weight of green tea leaves, 83 parts by weight of green tea leaves, 83 parts by weight of green tea leaves and 83 parts by weight of green tea leaves were mixed with 6.6 parts by weight of rice brown rice, 6.6 parts by weight of yeast extract And 166 parts by weight of cornstarch, and then 33 parts by weight of mineral concentrated water and 100 parts by weight of useful microorganisms were added and mixed. Then, 1600 parts by weight of deep sea water and 1,600 parts by weight of yellow clay were added.

Preparation Example 2. Preparation of the compound of step (C)

100 weight parts of Angelica keiskei koidz., 100 weight parts of Angelica keiskei, 100 weight parts of Angelica keiskei, 100 weight parts of ginseng, 100 weight parts of Seeds 100 weight part, 100 parts by weight of green tea, 100 parts by weight of green tea, 100 parts by weight of green tea, 100 parts by weight of green tea leaf, 100 parts by weight of duck, 100 parts by weight of blue wormwood and 100 parts by weight of horse mugwort were added After adding 25 parts by weight of sea tangle, 25 parts by weight of tortoiseshells and 25 parts by weight of anchovy syrup, and adding 10 parts by weight of the sea tangle, 25 parts by weight of mineral concentrated water was added, And 10000 parts by weight of deep sea water were added and mixed, followed by extraction at 80 ° C for 120 hours to obtain a hot water extract.

Example 1.

The compound of Preparation Example 1 was subjected to primary fermentation (inversion of the fermentation vessel once a day) at 30 ° C. for 14 days, then air was injected at the same temperature for 14 days to perform secondary fermentation Next, the secondary fermentation product was filtered and separated into a fermentation broth and a residue.

The residue and water were mixed at a weight ratio of 1: 2 and extracted at 80 DEG C for 20 hours to obtain a residue extract.

The filtered fermentation broth, the residue extract and the hot water extract of Preparation Example 2 were mixed at a weight ratio of 1: 0.5: 1.3 to prepare a cosmetic composition.

Comparative Example 1. Fermentation Omission

The compound of Preparation Example 1 and the hot-water extract of Preparation Example 2 were mixed at a weight ratio of 1: 1.3 to prepare a cosmetic composition.

Comparative Example 2. Omission of residue extract

The same procedure as in Example 1 was carried out except that the filtered fermentation broth without using the residue extract and the hot water extract of Preparation Example 2 were mixed at a weight ratio of 1: 1.3 to prepare a cosmetic composition.

Comparative Example 3: The hot-water extract of Production Example 2 was omitted

The same procedure as in Example 1 was carried out except that the filtered fermentation broth and the residue extract were mixed at a weight ratio of 1: 0.5 to prepare a cosmetic composition.

<Test Example>

Test Example 1. Cytotoxicity test

Cytotoxicity was measured according to the concentration of the cosmetic composition prepared in Examples and Comparative Examples.

Cytotoxicity was measured by the method of Rochem et al. In order to carry out the test in the safety concentration range of the sample. Cells (2 × 10 ⁵ cells / well) (RAW 264.7) were seeded in 24-well plates and cultured for 24 hours. After that, the medium was removed and 1 mL of the medium containing 3% of the serum in which the extract was dissolved was dispensed and cultivation was carried out for 24 hours. After 24 hours, 0.25 mL of XTT-PMS solution (1 mg XTT and 10 g PMS / mL of MEM without phenol red) was added to each well and incubated for another 2 hours. The degree of cytotoxicity was determined by measuring the absorbance of formazan at 450 nm using a microplate reader. The medium was MEM supplemented with 10% (v / v) fetal bovine serum (FBS), 0.5% (v / v) 50 g / mL streptomycin, 50 IU / mL penicillin, 0.125 g / mL fungizone, 3.024 g sodium bicarbonate And incubated at 37 ℃, 5% CO _2, 95% humidified air.

division Cell viability (%) 10 [mu] g / ml 25 [mu] g / ml 50 [mu] g / ml 100 [mu] g / ml Example 1 110.4 107.8 104.5 103.4 Comparative Example 1 57.5 51.6 44.1 35.6 Comparative Example 2 102.4 100.1 95.1 90.3 Comparative Example 3 103.8 102.4 98.5 90.5

As shown in Table 1, it was confirmed that the cosmetic composition prepared according to Example 1 of the present invention showed no toxicity in the interval of 10 to 100 占 퐂 / ml.

On the other hand, in Comparative Example 1, toxicity was observed in the entire section of 10 to 100 占 퐂 / ml, and in Comparative Examples 2 and 3, toxicity appeared in the section exceeding 25 占 퐂 / ml.

Test Example 2. Melanin formation inhibitory effect

To confirm whether the cosmetic composition prepared in Examples and Comparative Examples reduced the production of melanin pigment, B16-F10 cells (ATCC, Manassas, USA), a melanoma cell, each of the cosmetic compositions prepared in Examples and Comparative Examples 1 μM of α-MSH (melanocyte stimulating hormone), a representative inducer of melanogenesis, was treated and cultured for 3 days.

The melanin content of cultured melanoma cells was measured by washing with physiological saline, dissolution with lysis buffer, and treatment with 20% TCA solution. The precipitate was washed twice with 10% TCA solution, and then sequentially treated with a mixed solution of ethanol and diethyl ether (3: 1) and diethyl ether solution, and dried in air. Then, 0.85 M potassium hydroxide Solution and heated for 15 minutes. After cooling, the absorbance was measured at 440 nm. Proteins were quantified by the Bradford Method (Anal Biochem 1976; 72: 248-54) and the measured amount of melanin pigment was divided by the amount of protein to obtain 1 mg unit protein The measured amount of melanin pigment production (melanin pigment measurement amount / protein) is shown in Table 2 below. The control group was melanoma cells treated with the cosmetic composition of Example 1 without using? -MSH.

division Melamine concentration (%) α-MSH Control group albumin 10 [mu] g / ml 25 [mu] g / ml 50 [mu] g / ml 100 [mu] g / ml Example 1 59.2 100.3 98.1 85.1 60.3 24.5 18.2 Comparative Example 1 59.2 100.3 98.1 100.2 99.8 98.9 98.2 Comparative Example 2 59.2 100.3 98.1 100.1 97.4 95.2 92.5 Comparative Example 3 59.2 100.3 98.1 100.1 96.8 95.8 93.6

As shown in Table 2 above, it was confirmed that the cosmetic composition prepared according to Example 1 of the present invention shows a more excellent melanin production inhibitory effect than albumin which is a representative melanin formation inhibitor, from 10 ㎍ / ml or more.

On the other hand, in Comparative Example 1, the melanin production inhibitory effect was hardly observed in the whole section of 10 to 100 占 퐂 / ml, whereas in Comparative Examples 2 and 3, the melanin production inhibitory effect was weak in the whole section of 10 to 100 占 퐂 / Respectively.

Test Example 3: Inhibitory effect of tyrosinase activity

The effect of cosmetic composition on the activity of tyrosinase, which plays an important role in the formation of melanin pigment, was evaluated.

(DOPA) was oxidized to DOPA chrome by using a cell extract of melanoma cells treated with α-MSH and cosmetic composition of Test Example 2 was measured by a spectrophotometer at 475 nm . The reaction was carried out by adding the cell extract to a freshly prepared substrate solution (0.1% L-DOPA in 0.1 M sodium phosphate, pH 6.0) and incubating at 37 ° C. And tyrosinase activity was corrected by tyrosinase activity per 1 mg unit protein using the protein quantification method described in Test Example 2, and is shown in Table 3 below. As a control, melanoma cells were treated with ugly extract only without α-MSH.

division Tyrosinase activity (%) α-MSH Control group albumin 10 [mu] g / ml 25 [mu] g / ml 50 [mu] g / ml 100 [mu] g / ml Example 1 78.2 100.1 79.0 76.2 69.2 62.0 44.5 Comparative Example 1 78.2 100.1 79.0 99.0 98.4 96.6 94.1 Comparative Example 2 78.2 100.1 79.0 98.1 97.0 90.2 86.5 Comparative Example 3 78.2 100.1 79.0 98.4 94.6 89.1 85.0

The inhibitory effect of intracellular melanin production is shown by inhibiting the activity of tyrosinase, a key enzyme of melanogenesis.

As shown in Table 3 above, it was confirmed that the cosmetic composition prepared according to Example 1 of the present invention exhibited more excellent inhibition of tyrosinase activity than albumin from 10 μg / ml or more.

On the other hand, it was confirmed that Comparative Examples 1 to 3 inhibit tyrosinase activity not better than albumin in the whole interval of 10 to 100 占 퐂 / ml.

Test Example 4. Whitening Clinical Effect

100 subjects (20 persons per sample) suffering from pigmentation such as spots on the face were examined with respect to samples containing the present invention (Example 1, Comparative Example 1-3) and controls containing existing known whitening agents The sample (arbutin) was dissolved in propylene glycol / ethanol (8/2) to a concentration of 1%, and applied twice a day for 3 consecutive days in the morning and in the evening, twice daily. The extent of improvement of pigmentation after use was determined according to the following criteria.

◎: the ratio of the subjects showing excellent or effective effect is 80% or more

A: The ratio of the subjects showing excellent or effective effect was 50 to 79%

[Delta]: The proportion of the subjects showing excellent effect or effectiveness was 30 to 49%

X: Less than 30% of the subjects showed excellent efficacy or effectiveness

division Arbutin Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Whitening effect △ ◎ X △ X

As shown in Table 4 above, it was confirmed that the cosmetic composition prepared according to Example 1 of the present invention had excellent whitening effect.

On the other hand, Comparative Examples 1 and 3 showed almost no whitening effect, and Comparative Example 2 also showed low whitening effect.

Test Example 5. Moisturizing effect

To evaluate the moisture-holding ability of the cosmetic composition prepared according to Examples and Comparative Examples, a water retention ability test was conducted.

(0.03 g / 16 cm 2) was applied to each of 20 test subjects under a constant temperature and humidity room at 22 ° C. and a relative humidity of 45%, and then rubbed thoroughly. After 1 hour of application, 2 hours after application, And the results are shown in Table 5. < tb > < TABLE >

division Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Skin electrical conductivity Before application 6 6 6 6 After 1 hour of application 43 9 14 12 After 2 hours of application 37 7 10 9 Moisture Capability Rank One 4 2 3

As shown in Table 5, the cosmetic composition prepared according to Example 1 of the present invention showed much higher skin electrical conductivity at 1 hour and 2 hours after application than those of Comparative Examples 1 to 3. It can be seen that the composition prepared according to Example 1 is much superior to the cosmetic composition prepared according to Comparative Examples 1 to 3 in moisture content.

< Formulation example >

Hereinafter, formulation examples of the composition containing the extract of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation example 1. Manufacture of cosmetic products of emulsified formulations

Cosmetics of emulsified form such as nutrient lotion, cream, essence, and cosmetics of solubilized form such as softened longevity were prepared.

Emulsifier type cosmetics were prepared in the compositions shown in Table 6 below. The production method is as follows.

1) A mixture of raw materials 1 to 9 was heated to 65 to 70 占 폚.

2) The starting material of 10 was added to the mixture of step 1).

3) The mixture of raw materials 11 to 13 was completely dissolved by heating to 65 to 70 占 폚.

4) While the above step 3) was carried out, the mixture of step 2) was gradually added and emulsified at 8,000 rpm for 2 to 3 minutes.

5) The raw material of 14 was dissolved in a small amount of water and then added to the mixture of step 4) and further emulsified for 2 minutes.

6) The raw materials of 15 to 17 were each weighed, and then put into the mixture of step 5) and further emulsified for 30 seconds.

7) The mixture of step 6) was degassed after emulsification and cooled to 25-35 占 폚 to prepare an emulsifier-type cosmetic.

Furtherance Emulsifier type 1 Emulsifier type 2 Emulsifier type 3 One Stearic acid 0.3 0.3 0.3 2 Stearyl alcohol 0.2 0.2 0.2 3 Glyceryl monostearate 1.2 1.2 1.2 4 Wax 0.4 0.4 0.4 5 Polyoxyethylene sorbitan
Monolauric acid ester 2.2 2.2 2.2 6 Methyl paraoxybenzoate 0.1 0.1 0.1 7 P-hydroxybenzoic acid profile 0.05 0.05 0.05 8 Cetyl ethyl hexanoate 5 5 5 9 Triglyceride 2 2 2 10 Cyclomethicone 3 3 3 11 Distilled water ~ 100 ~ 100 ~ 100 12 Concentrated glycerin 5 5 5 13 Triethanolamine 0.15 0.15 0.15 14 Polyacrylic acid polymer 0.12 0.12 0.12 15 Pigment 0.0001 0.0001 0.0001 16 incense 0.10 0.10 0.10 17 The cosmetic composition of Example 1 0.0001 One 10

Formulation example 2. Solubilization Manufacture of Cosmetics for Formulation

Cosmetic products of the solubilized formulations were prepared with the compositions shown in Table 7 below. The production method is as follows.

1) 2 to 6 raw materials were put into 1 raw material (purified water) and dissolved using a mixer.

2) Raw materials 8 to 11 were completely dissolved in 7 raw materials (alcohol).

3) The mixture of step 2) was slowly solubilized by adding it to the mixture of step 1).

Furtherance Solubilization Formulation 1 Solubilization Formulation 2 Solubilization Formulation 3 One Purified water ~ 100 ~ 100 ~ 100 2 Concentrated glycerin 3 3 3 3 1,3-butylene glycol 2 2 2 4 EDTA-2Na 0.01 0.01 0.01 5 Pigment 0.0001 0.0002 0.0002 6 The cosmetic composition of Example 1 0.1 5 5 7 Alcohol (95%) 8 8 8 8 Methyl paraoxybenzoate 0.1 0.1 0.1 9 Polyoxyethylene
Hydro genide ester 0.3 0.3 0.3 10 incense 0.15 0.15 0.15 11 Cyclomethicone - - 2

Claims

(A) adding primary microorganism and mineral concentrated water as a solution of Hwasobyeol, Angelica grisea, Green tea leaf, Liliaceae, Rhus verniciflua, Gugija, Brown rice,
(B) injecting air under the same temperature as that of the primary fermentation to perform secondary fermentation;
(C) Peppermint, Ginseng, Ginseng, Seeds, Seeds, Zygomycetes, Saururus chinensis, Zygomycota, Zelgite strand mushrooms, Ganoderma, Golden, Green tea, Green tea leaves, Extracting the seaweed, the seaweed, the mineral water, the deep seawater, and hot water;
(D) separating the fermented product fermented in step (B) into a fermentation broth and a residue;
(E) hot water extraction of the residue separated in step (D); And
(F) mixing the fermentation broth separated in step (D), the extract extracted in step (E), and the extract extracted in step (C). A method for manufacturing a skin whitening cosmetic composition using a substance.

[3] The method according to claim 1, wherein 50 to 600 parts by weight of the group consisting of Angelica keiskei, green tea leaf, Japanese lobule, 5 to 10 parts by weight of brown rice; 5 to 10 parts by weight of yeast; 100 to 300 parts by weight of crude oil; 100 to 300 parts by weight of useful microorganisms; 20 to 100 parts by weight of mineral concentrated water; 1500 to 2000 parts by weight of deep ocean water; And 1500 to 2000 parts by weight of a yellowing agent. The method of manufacturing a skin whitening cosmetic composition using a natural-derived material comprising the leaves.

The method for preparing a skin whitening cosmetic composition according to claim 1, wherein the primary fermentation in step (A) is performed at 20 to 50 ° C for 12 to 20 days.

The method for preparing a skin whitening cosmetic composition according to claim 1, wherein the secondary fermentation is performed for 12 to 20 days in the step (B).

[7] The method according to claim 1, wherein 100 to 700 parts by weight of the leaf of the flower is 100 to 700 parts by weight of the group consisting of Angelica gigas Nakai, Ginseng, Seeds, Seeds, 100 to 800 parts by weight of a group consisting of zelkova mushroom, Ganoderma lucidum, golden, green tea, green tea leaves, dermis, purple wormwood and rhizome; 50 to 250 parts by weight of the group consisting of ginseng, gugija and oriental; 50 to 200 parts by weight of a group consisting of kelp, tar and sea anchovy; 5 to 20 parts by weight; 20 to 50 parts by weight of mineral concentrated water; And 1000 to 20,000 parts by weight of deep-sea water. The method of manufacturing a skin whitening cosmetic composition using the natural-derived material.

The method for preparing a skin whitening cosmetic composition according to claim 1, wherein the hot-water extract in step (C) is extracted at 70 to 90 ° C for 100 to 130 hours.

The skin-whitening cosmetic composition according to claim 1, wherein the mineral concentrated water in the steps (A) and (C) is concentrated in deep sea water to have a hardness of 230,000 to 290,000 mg / &Lt; / RTI >

The method of claim 1, wherein the hot-water extract in step (E) is obtained by mixing the residue and water at a weight ratio of 1: 1 to 3 and extracting the mixture at 70 to 90 ° C for 10 to 30 hours. A method for manufacturing a skin whitening cosmetic composition using a derived material.

2. The method according to claim 1, wherein the fermentation broth separated in step (D), the extract extracted in step (E), and the extract extracted in step (C) 3. The method for manufacturing a skin whitening cosmetic composition using a naturally-occurring substance comprising a papaya leaf.

9. A skin whitening cosmetic composition produced by the manufacturing method according to any one of claims 1 to 9.