KR102071218B1 - Anti-aging and anti-wrinkle of skin improving cosmetic composition comprising extracts of Moschus, Angelica gigas Nakai, Cornus officinalis, Cervi Parvum Cornu and Phellinus linteus, Ganoderma Lucidum, Inonotus obliquus, Grifola Frondosa, Sparassis crispa - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin wrinkles containing musk, Angelica, cornus, antler and green mushroom, ganoderma lucidum mushroom, chaga, leaf mushroom, matsutake mushroom extract as an active ingredient, musk, Angelica, cornus, antler and It contains extracts from situational mushrooms, ganoderma lucidum, chaga, leaf mushrooms and matsutake mushrooms as active ingredients, which help to anti-aging skin, improve skin wrinkles by promoting skin antioxidants, moisturizing, inhibiting elastase, inhibiting melanin formation and promoting collagen formation. It has an excellent effect on skin whitening.

Description

Anti-aging and anti-wrinkle of skin improving cosmetic composition comprising extracts of musk, Angelica, cornus, antler and green mushroom, ganoderma lucidum mushroom, chaga, leaf mushroom, zinnia mushroom as active ingredients of Moschus, Angelica gigas Nakai, Cornus officinalis, Cervi Parvum Cornu and Phellinus linteus, Ganoderma Lucidum, Inonotus obliquus, Grifola Frondosa, Sparassis crispa}

The present invention relates to a cosmetic composition for improving skin wrinkles containing musk, Angelica, cornus, antler and situation mushroom, ganoderma lucidum mushroom, chaga, leaf mushroom, matsutake mushroom extract as an active ingredient.

The skin is largely divided into three layers of epidermis, dermis and subcutaneous tissue in order from the outside, and functions to protect the organs of the body from temperature and humidity changes, ultraviolet rays, and other physical and chemical external stimuli. have. In particular, the epidermis plays an important role in preventing evaporation of moisture inside the human body. The epidermis is divided into the stratum corneum, granule layer, pore layer and basal layer in order from the outside, and the cells of the stratum corneum act like bricks and intercellular between keratinocytes. Lipids act like a mortar, forming the skin barrier.

In keratinocytes of healthy people, high concentrations of Natural Moisturing Factor (NMF) are present, which combines with moisture to inhibit the drying of moisture from the skin. However, these days, the artificial temperature control of cooling / heating according to the change of environment and life patterns, the skin stress caused by various stresses and environmental pollution that occur in social life, the frequent face wash and the age increase due to makeup habits Due to various causes such as skin aging, the moisture of the stratum corneum decreases, the skin becomes dry, the surface is rough, and the skin becomes crumbly and loses luster, so that the appearance of dull skin is required.

In addition, the main function of collagen, which is related to the health of the skin is known as the firmness of the skin, the resistance and binding of the tissue, the support of cell adhesion. The collagen has a thin skin due to aging and photoaging due to ultraviolet irradiation, and this phenomenon is known to be closely related to wrinkle formation of the skin.

In particular, if collagen metabolism is promoted by promoting collagen synthesis, the components of dermal matrix are increased, and retinoic acid, a collagen synthesis promoter, is expected according to the claim that the effect of wrinkle improvement, elasticity improvement, skin strengthening and wound healing can be expected. ), TGF (transforming growth factor), animal-derived placenta, beturinic acid (betulinic acid), chlorella extracts, etc. have been released a lot of products, but did not receive much attention.

In addition, many products containing collagen containing collagen as a cosmetic composition for skin protection are sold, but cosmetics applied to the skin surface have been pointed out that it is difficult to expect the moisturizing effect of the skin due to the difficulty of transdermal absorption of collagen, which is a polymer.

In addition, elastin fibers in the skin, together with collagen fibers, form crosslinks inside the dermis and are involved in skin elasticity. With age, the skin shows histologically deficient and aggregated elastin fibers, decreased collagen fibers, increased inflammatory cell infiltration, and a marked increase in the activity of the elastinase, a biochemical elastin degrading enzyme. Therefore, the elasticity of the skin is drastically lowered by the action of elastase, and sagging occurs. Elastase is the only enzyme known so far to break down elastin and its inhibition can fundamentally reduce skin aging.

In addition, melanin pigment, which is produced from melanocytes in the skin of the body, is a phenolic polymer material having a complex form of a black pigment and a protein, which blocks ultraviolet rays emitted from the sun to protect skin organs below the dermis and at the same time, freely generated in the skin living body. It plays a useful role in protecting proteins and genes in the skin, such as by holding radicals. The melanin produced by the internal and external stress stimulus is a stable substance that does not disappear until it is discharged to the outside through skin keratinization even when the stress disappears. However, when more melanin is produced than necessary, it causes hyperpigmentation such as blemishes, freckles, and spots, resulting in cosmetic consequences. Also, the increase in leisure population causes more people to enjoy working outside, There is an increasing demand to prevent melanin pigmentation.

On the other hand, the resonator stage was first recorded in the 'Sijae Baekilbangbang' written by Wang-gu of Song Dynasty period, and it is a prescription that was used for the purpose of preventing and treating book loss, which was used in many documents including 'Agreement Relief'. It consists of four herbs: Deer Antler, Cornus, Angelica and Musk.

Deer Antlers (Cervi Parvum Cornu) are dense and still hairs of bucks of C. neippon Temminck, C. elaphus.L, or C. canadensis Erxleben, belonging to deer and cervidae. The unhorned or slightly boned adult horns are cut and dried, traditionally used as a Chinese medicine. In addition, the main components of antler are lipids, proteins, carbohydrates, minerals and trace elements, etc. It is known that various physiological activities are expressed due to these components, and antler is best known for its tonic effect. It is known to treat the lower back and knees and weak symptoms. Studies on the physiological function of antler are actively conducted in the field of oriental medicine in Korea. , Liver function improvement, growth promoting action and diabetes treatment effect have been reported (Park PJ, et al, 2005).

Cornus officinalis is picked from the fruit of the Cornus tree, a deciduous tree of the Cornaceae, and picked and dried in the sun. It is reported that cornus as a Chinese medicine has a taste and is slightly warm and is good for liver and nerves, and has diuretic effect, blood pressure lowering effect, protein digestion effect, anticancer and antibacterial effect (Seo KI, et. al, 1999) In addition, the reported components of cornus acid include gallic acid, malic acid, tartaric acid, ursolic acid, morronoside, and loganine. (loganin), iridoid glycosides such as sweroside, and the like, and separation of tannins has also been reported (Park EB, et al, 2012). Studies on cornus oil have been reported for various functional studies such as antioxidant research, anticancer substance separation, antimicrobial activity separation, nutritional analysis (Lee KY, et al, 2009).

Angelica gigas Nakai is dried and harvested from the roots of apiaceae perennial plants before flowering. Angelica is known to have a blood-replenishing effect, which is known to be prescribed as a blood-reducing agent in anemia and gynecological diseases. The main component that shows the pharmacological action of Angelica gigas is decursin, a coumarin derivative, and decursinol, umbelliferon, and β-sitosterol. It is contained. The coumarin derivatives are known to be used for acute myocardial infarction as anticoagulants used to prevent hemorrhage in the past. It was found that decursin, the main component of Angelica gigas, exhibits anticancer activity and has an effect of enhancing the activity of protein kinase C. The fractions of Angelica gigas are human immune cells, B cells, T cells, and natural killer cells. (NK cells) have been reported to promote growth (Kim JH, et al, 2006).

Musk is a dried musk sac (pod) secretion from musk deer males. The active ingredient is muscone, cholesterol (cholesterol), calcium phosphate, ammonium carbonate, It contains fat, minerals, and fiber, and it has strong cardiovascular, anti-inflammatory, β-adrenaline effect, capillary permeability, antithrombin, and excitement. It has been used as an antidote to nausea and spermatozoa (Choi, YJ, et al, 1996).

The resonant stage as described above is expensive in medicine and is used for the same purpose by adding or subtracting other alternative drugs due to the authenticity controversy of some medicines or simplifying them as black circles (Hwang SY, et al, 2013). Looking at the use of the resonator stage, if you want to prevent aging and disease, if you are debilitated by surgery or a major illness, if your energy is degraded due to mental or physical overwork, if you are chronically tired and have a slow recovery from rest, Congenitally weak in the body, heavier and stinging due to lower blood circulation, dizziness and insomnia due to nervousness, excessive stress and drinking and weakened liver function, and also to relieve skin aging It is also known to be effective in improving skin wrinkles and having a skin whitening effect.

As a conventional technique used as a composition for skin cosmetics using the resonant stage as described above, the herbal mixture extract comprising antler, Angelica, cornus, and musk in Korea Patent Publication No. 10-2008-0073466 (August 11, 2008) A fermentation product of at least one selected from the group consisting of alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants and perfumes; Compositions consisting of one formulation selected from the group consisting of essences, lotions, creams, packs, gels, ointments, patches, and sprays have been known.

 However, because only the resonant stage has some antioxidant effects, skin wrinkle improvement effect, and skin whitening effect, the fundamental skin moisturizing function, elastase inhibitory function, melanin formation inhibiting function and collagen formation promoting function are not sufficient, At present, there is a limit to the use as a skin cosmetic composition such as cosmetics and soap.

Patent Document [001] Korea Patent Publication 10-2008-0073466 (August 11, 2008)

In order to solve the above problems, musk, Angelica, Cornus, Deer Antler and Situation mushroom, Ganoderma lucidum mushroom, Chaga, Leaf mushroom, Blossom mushroom extract containing as an active ingredient skin antioxidant, skin moisturizing, elastase inhibition, It is an object of the present invention to provide a skin cosmetic composition having anti-aging skin, improving skin wrinkles, and skin whitening through inhibiting melanin formation and promoting collagen formation.

The present invention to solve the above problems, musk, Angelica, Cornus, Deer Antler and Situation mushrooms, Ganoderma lucidum, Chaga, Leaf mushrooms, Blossom mushroom containing a mushroom extract as an active ingredient It is done.

The skin anti-aging and anti-wrinkle cosmetic composition is mixed with the musk, Angelica, cornus and antler extract and the situation mushrooms, ganoderma lucidum mushroom, chaga, leaf mushrooms, matsutake mushrooms in a 1: 1 to 2 weight ratio and 2 to 10 Dilution with pear purified water is a solution to the problem.

The musk, Angelica, Cornus oil, Deer Antler extract are finely pulverized each material to a size of less than 400 mesh, ethanol in the ground material, immersed for 1 to 10 days and then extracted, filtered to recover the extract, Ratary What is obtained by distillation under reduced pressure using an evaporator is a means for solving the problem.

The situation mushroom, ganoderma lucidum mushroom, chaga mushroom, leaf mushroom, matsutake mushroom extract is finely pulverized each material, ethanol in the ground material and extracted using a cryogenic pressure extractor for 4 hours at 40 ℃, then filtered by extract Is recovered and distilled under reduced pressure using a ratary evaporator as a means of solving the problem.

The cosmetic composition for improving skin wrinkles is one selected from the group consisting of lotion, gel, water-soluble liquid, cream, essence, oil-in-water or water-in-oil type makeup foundation, foundation, skin cover, face powder, and two-way cake By means.

Musky, Angelica, Cornus, Deer Antler and Situary Mushroom, Ganoderma Lucidum Mushroom, Chaga Mushroom, Leaf Mushroom, Blossom Mushroom Extract Containing Skin Composition for Skin Wrinkle Improvement, Musk, Angelica, Cornus, Deer Antler and Sacred Mushroom , Ganoderma lucidum, chaga, leaf mushroom, matsutake mushroom extract as an active ingredient, skin anti-aging, skin wrinkle improvement, skin whitening through skin antioxidant, skin moisturizing, elastase inhibition, melanin formation inhibition and collagen formation promotion It has an excellent effect.

1 is a graph showing the total melanin production inhibition rate of the situation mushroom
Figure 2 is a blood circulation improvement effect of Ganoderma lucidum
3 is a graph showing the cell activation effect of chaga
4 is a graph showing the collagen production promoting effect of the leaf mushroom
5 is a graph showing the moisturizing effect of zinnia mushroom

The present invention is characterized in that the technical composition of skin wrinkle improvement cosmetic composition containing musk, Angelica, cornus, antler and green mushroom, ganoderma lucidum mushroom, chaga, leaf mushroom, matsutake mushroom extract as an active ingredient.

The cosmetic composition for improving skin wrinkles is a mixture of musk, Angelica, cornus and antler extract and the situation mushroom, ganoderma lucidum mushroom, chaga, leaf mushroom, zinnia mushroom extract in a 1: 1 to 2 weight ratio and purified water of 2 to 10 times Diluted with is characterized by the technical configuration.

The musk, Angelica, Cornus oil, Deer Antler extract are finely pulverized each material to a size of less than 400 mesh, ethanol in the ground material, immersed for 1 to 10 days and then extracted, filtered to recover the extract, Ratary What is obtained by distillation under reduced pressure using an evaporator is characterized by a technical configuration.

The situation mushroom, ganoderma lucidum mushroom, chaga mushroom, leaf mushroom, matsutake mushroom extract is finely pulverized each material, ethanol in the ground material and extracted using a cryogenic pressure extractor for 4 hours at 40 ℃, then filtered by extract Was recovered and vacuum distillation using a ratary evaporator is characterized by the technical configuration.

The cosmetic composition for improving skin wrinkles is one selected from the group consisting of lotion, gel, water-soluble liquid, cream, essence, oil-in-water or water-in-oil type makeup foundation, foundation, skin cover, face powder, and two-way cake It features.

Hereinafter, the present invention will be described in detail with reference to embodiments of the present invention so that those skilled in the art may easily implement the present invention. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention.

First, the cosmetic composition for improving skin wrinkles of the present invention contains musk, Angelica, cornus, antler and situation mushrooms, ganoderma lucidum mushroom, chaga, leaf mushrooms, matsutake mushroom extract as an active ingredient.

The musk, donkey, cornus and antler are as described above as the resonant stage component, and the resonator stage component is used as it is in the present invention, and the addition of five kinds of mushroom extracts is a key technical configuration of the present invention.

Mushrooms are more than 90% moisture, the remaining 10% is composed of protein (2 to 3%), fat, sugar, minerals. Almost no calories and high protein is considered as a very good food to prevent diet and adult diseases. Mushrooms are rich in vitamins and minerals, and vitamins B2, niacin and provitamin D. Vitamin B2 is important for the metabolism of fats, proteins and sugars, and is essential for promoting growth. In dermatological terms, niacin prevents dermatitis, and provitamin D changes intake into vitamin D in the body. Vitamin D is essential for preventing skin cancer, making bone tissue, preventing osteoporosis, and essential for developing bones and teeth in infants. Potassium is the most of the minerals, potassium is the cause of the increase in blood pressure, so sodium falls out of the body, it is excellent in preventing hypertension. In addition, minerals contain a lot of phosphorus, which is an important component of bones and teeth.

Among the mushrooms, the situation mushrooms [phellinus linteus (Ikekawa, T,: Antitumor action of some basidiemycetes, especially Phellinus Linteus GANN, 59: 155, l968)] have been introduced in China since ancient times as parasitic to mulberry trees, According to this, it is also found that wild leaves are also wild (Cha Dong-ryul, monthly subsidiary p34-37,9512). In Korea, the situation mushroom refers to the woody mud (phellinus linteus) and belongs to the pine scale Hymenochaetaceae.

About 48 species of mud mushrooms belonging to this situation are reported around the world, but in Korea, rust muddy mushrooms (P. ferruginosus), dried mud mushrooms (P. gilvus), wormwood mud mushrooms (P. igniarius), and branched mushrooms ( Eight species were collected from P. laevigatus, P. pomaceus, P. robustus, fir mud and P. pini.

Mushrooms belonging to these mud mushrooms have been used for the treatment of gynecological diseases such as uterine bleeding and menstrual irregularities under the names of differentiation, sedimentary coliform, and hawthorne. According to various researchers, it is known that the tumor suppression rate is 60-97% in animal experiments, and it is excellent in cancer treatment effect. Adult disease treatment. Immune function. This is excellent and has the effect of suppressing the formation of blemishes.

In particular, as shown in Figure 1, the situation mushroom is known to suppress the total melanin production in the cell to remove the blemishes of the skin to make the skin bright and transparent, so in the present invention mixed the situation mushroom extract for this purpose use.

That is, as shown in Figure 1, in the graph showing the inhibition rate of total melanogenesis in cells using B16F1 melanoma, 3.00% of the situation mushroom extract showed the same whitening efficacy as the treatment with arbutin (0.2%), It can be confirmed that the situation mushroom extract exhibits an excellent melanin synthesis inhibitory effect.

Ganoderma lucidum (Fr) Karst is a perennial mushroom belonging to the Polyporaceae family. It is a supernatant to the main and new agricultural plants of Lee Si-jin, the best herb in China, more than 2,000 years ago. It is described as effective for the treatment of arthritis, remission, bronchitis and the like. In oriental medicine, it has been used in combination with other herbal medicines such as arteriosclerosis, hypertension, stroke, angina pectoris, various cancers, myasthenia gravis, acute bronchitis, etc. because of its pharmacological effects such as nourishing tonic, detoxification convergence, droplets and blood lipid reduction.

On the other hand, research papers on the medical efficacy of Ganoderma lucidum mushrooms include anti-cancer effects, immuno-stimulating effects and wound healing effects (Ganoderma lucidum polysaccharide accelerates refractory wound healing by inhibition of mitochondrial oxidative stress in type 1 diabetes Tie L, Yang HQ, An Y, Liu SQ, Han J, Xu Y, Hu M, Li WD, Chen AF, Lin ZB, Li XJ Cell Physiol Biochem 2012; 29 (3-4): 583-94 Epub 2012 Apr 3). (Eur Rev Med Pharmacol Sci 2012 Feb; 16 (2): 166-75 Hypoglycemic effect and mechanism of a proteoglycan from ganoderma lucidum on streptozotocin-induced type 2 diabetic rats Teng BS, Wang CD, Zhang D, Wu JS, Pan D, Pan LF, Yang HJ, Zhou P)

In particular, Ganoderma lucidum is high in amino acids, water-soluble proteins, steroids, alkaloids, vitamins, etc., so it does not age and is used for central nervous system analgesic, hemolytic anemia, arteriosclerosis, and thyroid gland. Since it has the effect of nourishing the skin to nourish, the present invention uses a mixture of Ganoderma lucidum extract for this purpose.

In other words, as shown in [Figure 2], as the effect of improving the blood circulation of certain Ganoderma lucidum extract with a DIRI device of dorex Co., Ltd. Placebo (left, Ganoderma lucidum extract 0.0%) and (Right, Ganoderma lucidum extract 5.0%) After measuring the skin temperature after 30 minutes, the average temperature was increased from 23.64 ℃ to 25.27 ℃, it can be seen that as the skin temperature is higher blood circulation is promoted.

Chaga (Inonotus-obliquus or Fuscoporia obliqua) is an extremely cold-tolerant mushroom of the genus Fuscoporia-SPP, the closest lineage of Hymenochaetaceae and Mucropor eceae. Acids, horomogens, phenol groups, polyphenols, oxyphenolcarboxylic acids, ignocene, chaga acids (60%), 6-8 polysaccharides, lactic acid, lignin, magnesium, and the like, and other aluminum oxides, iron, silicon, It contains calcium, cupric oxide, manganese, zinc, sodium and the like.

Chaga is black in color and usually takes 10-15 years to reach 40 cm in length, 15 cm in circumference, and 5 kg in weight. The junctions on chaga's trees have a soft light color and are cold-tolerant mushrooms found mainly in cold, deep cold areas north of 45 degrees north latitude. Chaga is found on Earth in Ukraine, Siberia, Russia, and Kamchatka. It is known as Peninsula, Yeonhae, parts of Mongolia, parts of Heilongjiang, China, northern Hokkaido, Norway, Finland, Sweden and Japan, but it is rarely found in Korea and also found in Canada, Northeastern US An example is reported. In addition, chaga is a spore penetrating through the wound part of the living birch germinates when the temperature, moisture, acidity, etc. are appropriate, the mycelium grows. This part is called the bacterium. The part of the bacterium is generally called chaga, and in the fruiting body of the bark where the bacterium is formed, spores are formed and multiply and grow until the tree dies.

Chaga mushroom has excellent anti-cancer effects, and it has anti-HIV effect by water-soluble lignin and anti-influenza virus proliferation effect as a diabetic treatment, antioxidant action, and immunity strengthening function.

In particular, chaga mushroom is known to have an effect of promoting the activity of fibroblasts and promote the production of elastin to have an excellent effect on skin elasticity recovery, so the chaga mushroom extract is used for this purpose.

That is, as shown in FIG. 3, when the fibroblasts were treated with human fibroblasts in the growth stop state (growth 0%), the activity promoting effect of the fibroblasts constituting the dermis layer was 2.0 to 3.0%. It showed 140 ~ 150% better cell activity than cells treated with Buffer only. These experimental results are to promote the production of elastin as the cell activity is promoted it can be seen that shows excellent results in skin elastic recovery.

Leafy mushrooms (Grifola frondosa Fr) are taxonomically belong to Aphllophorales, Polyporaceae, and Grifola. Leaf fungus nuclei include antipyretic, edema, and anti-tumor tumors. It is used as a medicinal herb because of its medicinal properties. Among the edible mushrooms, leafy mushrooms contain not only ingredients necessary for physiological control of the living body such as sugars, proteins, fatty acids, organic acids, amino acids, nucleic acids, enzymes, and inorganic salts, but also abundant flavor components. It is excellent in taste and form and is treated as one of the high-grade mushrooms useful as food.

 In addition, the chemical structure of beta glucan, the major polysaccharide of leaf mushrooms, has been reported to be the most potent component of stimulating cellular immune responses, unlike the structure of beta glucan found in other medicinal mushrooms (Chem Pharm Bull, 34 (4), 1709-1715, Nippon Shokuhin Kogyo Gakkaishi, 41 (10), 733-740)

In addition, leaf mushrooms are known to be effective in lowering blood pressure, diabetes, obesity, reducing blood cholesterol, antibacterial, diuretic, tonic, anti-anemia and pulmonary bleeding as well as anti-cancer activity (Critical Review in Immunology, 19, 65-96)

In particular, the leaf mushroom is rich in heteroglucan, peptide glucan, niacin is excellent in antidiabetic, breast cancer, lung cancer, and peptide glucan has the effect of inhibiting wrinkle formation by promoting collagen synthesis in the present invention for this purpose Use a mixture of mushroom extracts.

That is, as shown in Figure 4, after irradiating 100mJ / cm ² ultraviolet rays to human fibroblasts, collagen synthesis results. Cell group treated with leaf mushroom extract (Blue bar) showed more than twice the collagen synthesis promoting effect than the cell group (Gray bar).

Spamula mushroom (Sparassis crispa) is a fungus of the genus Pleurotus eryngii (Pleurotus eryngii). The fruiting body of the mushroom is 5 ~ 20cm or more in size, and each branch is fleshy and has a white wave-shaped flower shape. Near the roots of living trees in the forest, dead stems, stumps, etc.

Blossom mushroom is one of the parasitic mushrooms in Korea's coniferous resources. It is a high-temperature mushroom that grows from May to August mainly in pine, pine, larch and fir. It was initially known as a fungus that grows on coniferous roots and reduces the value of wood. In 1997, Fukushima, a Japanese high school teacher, developed the sawdust bottle cultivation method. The main producing regions are Paju, Yeoncheon, and Uiwang, Gyeonggi-do, and Japan, China, Europe, and the Americas overseas. It was confirmed that it contains a large amount of beta glucan (β-glucan) as the main ingredient, attracted worldwide attention, and also includes vitamin D and vitamin B2. Blossom mushrooms are used for food because of their solid flesh and good texture, and they are widely used for medicinal use because they are recognized for their various functionalities. Currently, most of them are produced by artificial cultivation indoors.

In particular, the flower mushroom is considered to be the best luxury mushrooms for health promotion by containing a large amount of beta glucan and excellent skin moisturizing effect is known, the present invention uses a mixed leaf mushroom extract for this purpose.

That is, as shown in FIG. 5, the product was applied to the inside of the lower arm of the arm at a temperature of 22C and humidity (45-50%), and the skin moisture content was measured at 30-minute intervals, and the flower glucan was 20% BG. It can be seen that it shows more excellent moisturizing power.

The cosmetic composition of the present invention, the musk, Angelica, Cornus and Deer Antler extract and the situation mushroom, Ganoderma lucidum mushroom, chaga, leaf mushrooms, matsutake mushroom extract in a 1 to 2 weight ratio and 2 to 10 times purified water It is preferable to use diluted with.

At this time, the musk, Angelica, Cornus oil, Deer Antler extract, finely pulverized each material to a size of less than 400 mesh, ethanol in the ground material, immersed for 1 to 10 days and then extracted, filtered to recover the extract solution It is prepared by distillation under reduced pressure using a ratary evaporator.

In addition, the situation mushroom, ganoderma lucidum mushroom, chaga mushroom, leaf mushroom, matsutake mushroom extract, after crushing each material finely, put ethanol in the ground material and extracted for 4 hours at 40 ℃ using a cryogenic pressure extractor, and then filter The extract is recovered and prepared by distillation under reduced pressure using a ratary evaporator.

The cosmetic composition for improving skin wrinkles may be one selected from the group consisting of lotion, gel, water-soluble liquid, cream, essence, oil-in-water or water-in-oil type makeup base, foundation, skin cover, face powder, and two-way cake. to be.

[Production of Cosmetic Composition of the Present Invention]

20 g of musk, 100 g of Angelica, 100 g of cornus oil, and 100 g of antler, respectively, are finely pulverized to less than 300 mesh. A brown extract was prepared by distillation under reduced pressure using an evaporator.

Next, 100 g of mushrooms, 100 g of Ganoderma lucidum mushroom, 100 g of chaga mushroom, 100 g of leafy mushroom, 100 g of matsutake mushroom, respectively, finely pulverized, 1 liter of ethanol was added to the ground material and then used for 4 hours at 40 ° C. using an ultra low temperature extractor. After extraction, the extract was filtered to recover the extract, and a brown extract was prepared by distillation under reduced pressure using a ratary evaporator.

The musk, Angelica, Cornus and Deer Antler extracts, Sichuan mushroom, Ganoderma lucidum mushroom, Chaga mushroom, Leaf mushroom, and Mushroom extract were mixed in a 1: 1 weight ratio and diluted with 5 times purified water to prepare a cosmetic composition of the present invention. .

[Measurement of Antioxidant Effect of Cosmetic Composition of the Present Invention]

In order to measure the antioxidant effect of the cosmetic composition prepared in Example 1, a free radical scavenging activity test was conducted. Specifically, three tubes containing 1 ml of a 02 mM DPPH (1,1-diphenyl-2-picryhydrazyl, Sigma) solution were prepared. One tube was added purified water as a control, the other two tubes were added to each of the situation mushroom extract and the cosmetic composition of the present invention as an experimental group, respectively. Then, after leaving each of the three tubes for 10 minutes at room temperature, the absorbance was measured at 525 nm for the three tubes, and then the free radical scavenging rate of the experimental group was calculated using the equation. The results are shown in the following [Table 1].

% Free radical scavenging ratio = [100-{(absorbance of experimental group-absorbance of control group) / absorbance of control group} × 100]

Experimental group Free radical scavenging rate (%) Situation Mushroom Extract 22.4 Cosmetic composition of the present invention 44.5

As shown in [Table 1], since the cosmetic composition of the present invention exhibits free radical scavenging rate of about 2 times or more than the situation mushroom extract, from this, the cosmetic composition of the present invention has a much superior antioxidant effect than the situation mushroom extract. Able to know

[Measurement of Melanin Production Inhibition Effect of Cosmetic Composition of the Present Invention]

A 24-well plate medium was prepared in which Dulbecco's modified Eagle's media (DMEM) was added 10% fetal bovine serum. Rat cells were inoculated with B16 melanoma cell line at a concentration of 10 ⁵ cells / well and cultured under 37 ° C. and 5% CO 2 conditions. Subsequently, after confirming the adhesion of the pigment cells, the cosmetic composition and the situation mushroom extract prepared in [Example 1] as an experimental group were added to the medium, respectively, and cultured for 3 days, respectively. In addition, medium without addition was incubated together for 3 days. Cultures were removed from the experimental and control groups, respectively, and washed with PBS. Subsequently, the pigment cells were dissolved with 1N sodium hydroxide and absorbance was measured at 400 nm, and then the melanin production inhibition rate was calculated according to the following equation. The results are shown in the following [Table 2].

Melanin production inhibition rate (%) = [100- (absorbance of experimental group / absorbance of control group) × 100]

Experimental group Melanin production inhibition rate (%) Situation Mushroom Extract 24.5 Cosmetic composition of the present invention 52.4

As shown in the above [Table 2], the cosmetic composition of the present invention can be seen that the effect of inhibiting the melanin production of pigment cells about 2 times or more than the situation mushroom extract.

[Measurement of Fibroblast Collagen Biosynthesis Increasing Effect of Cosmetic Composition of the Present Invention]

A 96-well plate was prepared in which 10% fetal bovine serum was added to Dulbecco's modified Eagle's media (DMEM). Human fibroblasts were inoculated onto the plate at a concentration of 5,000 cells / well, and cultured at 37 ° C. and 5% CO 2 until 70% to 80% of the cells were grown. Example 1] The cosmetic composition and the situation mushroom extracts prepared in each were added and incubated for 1 day each. Each of the cultured cell cultures was collected and the degree of collagen production was measured using a commercially available collagen biosynthesis measuring instrument (Takara Shuzo Co, Ltd Catalog #: MK101).

Specifically, the collected cell culture solution was placed in a 96-well plate uniformly coated with primary collagen antibody, and then subjected to an antigen-antibody reaction in a thermostat for 3 hours. Thereafter, chromophore-conjugated secondary collagen antibody was placed in a 96-well plate and reacted again for 15 minutes. Then, the color causing substance was added to cause color development at room temperature for 15 minutes. Subsequently, 1M sulfuric acid was added to stop the color development. Absorption was measured at 450 nm of the 96-well plate, which stopped color development, using a light absorber, and the degree of collagen production was calculated according to the following equation. The results are shown in the following [Table 3]. Absorbance of the control group was measured using a plate to which nothing was added.

Collagen production rate (%) = (reaction absorbance of experimental group / reaction absorbance of control group) × 100

Experimental group Collagen production rate (%) Situation Mushroom Extract 20.5 Cosmetic composition of the present invention 60.4

As shown in Table 3, the cosmetic composition of the present invention is much superior to the collagen production rate than the situation mushroom extract, it can be seen that the collagen biosynthesis effect is much superior.

The above description is merely illustrative of the technical spirit of the present invention, and those skilled in the art to which the present invention pertains may make various modifications and changes without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention but to describe the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

Claims (5)

delete Musk, Angelica, Cornus and Deer Antlers are finely pulverized to a size of less than 400 mesh, and the ground materials are mixed, ethanol is added, immersed for 1 to 10 days, extracted and filtered to recover the extract, and the ratary evaporator is used. Extract obtained by distillation under reduced pressure; Sifted mushrooms, ganoderma lucidum mushroom, chaga mushroom, leaf mushroom and matsutake mushroom, respectively, finely pulverized, mixed with crushed ingredients, ethanol was added and extracted for 4 hours using a cryogenic pressure extractor, and then the filter was recovered. And extracts obtained by distillation under reduced pressure using a ratary evaporator; mixed with a 1: 1 to 2 weight ratio and diluted with purified water having a weight of 2 to 10 times the weight of the mixed cosmetic composition for improving skin wrinkles
delete delete The method of claim 2,
The cosmetic composition for improving skin wrinkles is skin care, gel, water-soluble liquid, cream, essence, oil-in-water or water-in-oil makeup base, foundation, skin cover, face powder, or two-way cake

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