KR101803757B1 - Cosmetic composition containing natural complex fermented extracts - Google Patents

Abstract

The present invention provides a method for producing a herbal medicine, comprising the steps of: obtaining a herbal medicine extract selected from at least one group selected from the group consisting of a root chamber, a windblown cloth, a gardenia fruit, a rhizome, a rhizome seedling, a rhus vernacolor, a briar ripened seed, Culturing the above extract with a pre-culture medium of mycelia of leaf mushroom or mycelium of leaf mushroom, removing the mushroom mycelium from the present culture to obtain a fermented extract of herbal medicine; The present invention relates to a cosmetic composition comprising as an active ingredient a complex fermented extract of herbal medicines obtained from the step of removing the mung bean curd from a culture solution obtained by culturing the fermented extract of herbal medicines mixed with Jeju Ome stock, It exerts excellent effects on skin improvement such as synthesis promotion effect, skin wrinkle improving effect, whitening effect, moisturizing effect, skin irritation alleviation effect, acne prevention or alleviation effect, atopic improvement effect and anti-inflammatory effect.

Description

The present invention relates to a cosmetic composition containing a complex fermented extract of herbal medicines as an active ingredient,

The present invention relates to a cosmetic composition comprising a complex herbal composition fermented extract as an active ingredient, and more particularly to a cosmetic composition containing a fermented extract of a herbal medicinal composition as an active ingredient, and more particularly to a cosmetic composition containing a crude herbal extract, And extracts of two or more kinds of mushrooms are added to and extracted from the herbal medicinal extracts containing the sole extract or two or more mixed extracts of the herbal medicines, and the mixed fermented extracts of the herbal medicines obtained as the active ingredient To a composition for external application for skin.

Skin exposed to ultraviolet rays produces free radicals and reactive oxygen species (ROS), leading to skin cancer, phototoxicity, autoimmune disorders, photosensitivity and skin aging (Norins, J. Invest. Dermatol., 39 : 445, 1962; Cadenas, Ann. Rev. Biochem., 58: 79, 1989). Synthesis and degradation of collagen-like extracellular matrix in vivo are appropriately controlled, but as the aging progresses, the synthesis gradually decreases and the expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, is promoted, And wrinkles are formed. These degrading enzymes are also activated by ultraviolet irradiation. Therefore, there is a demand for development of a substance capable of regulating MMP expression induced in the cell or inhibiting its activity.

Skin disease refers to abnormalities appearing in all the skin including the hair, nails and claws of mammals including humans. Atopic dermatitis, which is present in people with atopic allergy, is a typical skin disease. The cause of atopic dermatitis has not yet been clarified. However, atopic dermatitis has a history of atopic dermatitis in about 70% of the patients, and it is well developed in people with allergic constitution. Especially, allergic rhinitis, It is understood as one of factors or immune diseases. Atopic dermatitis can be aggravated depending on the mental instability and emotional state of the patient, especially when the skin is scratched by the initial severe itching and secondary infection occurs. Atopic dermatitis affects a considerable number of people, suffering from 0.5-1% of the total population and 5-10% of children. Symptoms usually appear within 2 to 6 months of age, especially in infants less than 1 year of age, and 85% are within 5 years of age. Atopic dermatitis often occurs in infancy, but in recent years, the number of patients after puberty has increased, and the medical history has also become very long. In the treatment of atopic dermatitis, medicines such as corticosteroids which can expect a high pharmacological effect are used. However, it is well known that the use of such corticosteroids results in rebound (weakness of the dramatic lesion that occurs after drug withdrawal). As a countermeasure against such side effects, a non-steroidal anti-inflammatory agent or an antihistamine agent which does not contain a hormone such as adrenocortical hormone is used but it is difficult to cure. Therefore, it is very urgent to develop a medicament for relieving or treating atopic dermatitis without side effects.

The skin of a person is constantly changing with age. Skin aging is largely divided into natural aging (or endogenous aging) and external aging (EB Doris, Cosmetics & Toiletries, 111, 31-37, 1996). Natural aging (endogenous aging) While control is difficult, external aging is relatively easy to manipulate because it is affected by environmental factors. Exemplary external aging factors include ultraviolet light and the most prominent external aging phenomenon is wrinkle formation (HW Daniell, Ann Intern Med, 75, 873-880, 1971, GL Grove, Jamecad Dermatol, 21, 631-637 , 1989, CE Griffiths et al, Arch Dermatol, 128, 347-351, 1992). On the other hand, skin exposed to ultraviolet rays generates free radicals and reactive oxygen species, leading to skin cancer, phototoxicity, autoimmune disorders, photosensitivity and skin aging (Norins, J. Invest. Dermatol., 39: 445, 1962; Cadenas, Ann. Rev. Biochem., 58: 79, 1989). The synthesis and degradation of extracellular matrix such as collagen in vivo is appropriately controlled, but its synthesis is reduced as aging proceeds, and the expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, is promoted, And wrinkles are formed.

On the other hand, melanin is converted from tyrosine to dopa and dopaquinone by the action of tyrosinase existing in pigment cells, and is generated by dopachrome. Melanin protects the body from ultraviolet light and has an important function in regulating hormone secretion in the body. However, when melanin is overproduced, it is known that it forms spots and freckles, promotes skin aging, and plays an important role in skin cancer induction. Therefore, research and development are actively conducted to prevent melanin overproduction.

(Japanese Patent Laid-open Publication No. 4-9320), hydroquinone (Japanese Patent Publication No. 6-192062), kojic acid (Japanese Patent Laid-open No. 56-7710), arbutin Which are used as whitening cosmetics. However, these cosmetics are poor in stability in cosmetic formulations and are decomposed to cause discoloration, odor generation, efficacy at the level of the living body, unclear effects or safety, and their use is limited It is true.

Recently, attention has been focused on functional cosmetics having functions such as antioxidation, wrinkle relieving and itching relief obtained from natural extracts in order to reduce skin irritation caused by various chemical substances and the like. In addition to low adverse effects on the skin, natural materials have recently become highly appreciated as a raw material for cosmetics due to the increasing popularity of cosmetics using natural materials. For example, U.S. Pat. No. 5,972,341 describes the wrinkle-reducing effect of the Commiphora plant, particularly the Commiphora mukul extract. Japanese Patent Application Laid-Open No. 9-672662 describes a seaweed extract having hyaluronidase inhibitory activity. Japanese Patent Application Laid-Open No. 10-85905 discloses an effect of improving skin texture of fucoidan extracted from seaweed. Japanese Patent Laid-Open No. 2-245087 describes the antioxidative effect of Sargassum sp. Extract. Japanese Patent Laid-Open No. 3-294384 discloses an antioxidant composition extracted from a genus of Hizikia. Japanese Patent Application Laid-Open No. 7-10769 discloses a Phaeophyceae extract having a converging effect. Korean Patent No. 0431076 discloses a cosmetic composition for improving healing of atopic skin including a white lotus, a jasmine, an echinacea and a fermented soybean extract, and Korean Patent No. 0511494 discloses a cosmetic composition for treating atopic dermatitis including dysentery, Korean Patent Application Laid-Open No. 2004-0065126 discloses an extract and composition for treating and preventing atopic skin including herbal plant extracts obtained from natural gingko mushrooms, yugun pei, licorice, white bream, And the like. Also, Korean Patent Application Publication No. 2004-0011584 discloses a composition excellent in the treatment of atopic skin diseases, which is formed by mixing and mixing natural ingredients such as licorice, licorice, safflower and lard (pig scaffold).

Nuruk consists of various microorganisms such as wheat bran and rice containing starch and protein, especially microorganisms having excellent saccharification function of starch, and they secrete extracellular enzymes and produce by the process of proliferation of yeast using sugar produced by this enzyme do. When the yeast is dried, the enzyme is kept in a dry state and the yeast is in a dormant state. When water and starch are added, starch glycation and alcohol fermentation are promoted. Homo lactic acid bacteria and yeast (Saccharomyces coreanus, Aspergillus oryzae, Asp Luchuensis mut. Kawachii, Asp awamori) are predominantly composed of yeast fungus grown in yeast (Aspergillus oryzae, Saccharomyces cerevisiae (Saccharomyces crerevisiae) coexist together and fermentation occurs. The Patent Documents on Jeju Jujube have reported antistress compositions [Korean Patent Laid-open No. 10-2014-0072421], compositions for improving wrinkles [Korean Patent Laid-open Publication No. 10-2014-0072419], etc., and cosmetic compositions containing plant extracts [Korean Patent Registration No. 10-0971629], and the like.

On the other hand, the active ingredient of herbal medicine has high absorption rate and bioavailability, but the extract obtained by simple extraction shows effective efficacy in vitro, but in the case of external preparation for skin containing the extract, the effect is not satisfactory. I did.

However, the fermentation broth extract does not use an organic solvent but uses a microorganism useful for the human body to bind glycosides existing in the herbal medicine to glucose molecules having relatively high molecular weights as aglycones of low molecular weight It is possible to obtain an effect that the effective ingredient passes through the stratum corneum more easily and is effectively absorbed into the skin.

Thus, there is a further need for development of a new external preparation for skin using a fermented extract of a herbal medicinal product which is low in toxicity inherent to herbal medicines and safe for skin irritation and maximizing effective efficacy of the skin.

The inventors of the present invention have confirmed that a flavonoid (Flavonoid) having an efficacy effect in an extract of a fermented herbal medicine cultured by adding mycelia of Leaf mushroom to an extract extracted from a crude drug mixture in Korean Patent No. 10-1081913 is an unglycosylated component.

In addition, the conventional fermented herbal medicine extract has a high viscosity polysaccharide component of water-soluble polysaccharide, which is highly viscous due to salt and pH, and is in contact with alcohols and gelatinized. In addition, polysaccharides are difficult to transdermally permeate due to hydrophilic high molecular weight molecular structure, and thus various physiological activities that can be expected through oral administration are difficult to be obtained through transdermal application, which is a form of use of cosmetics.

Accordingly, it is an object of the present invention to overcome the disadvantages of the cosmetic comprising the above-mentioned natural product extract as a raw material.

Therefore, in order to solve the above problems, the present inventors have found that, when decomposing the polysaccharide of the fermented herbal extract without using an acid decomposition method using an organic solvent that affects the stability of the cosmetic, In addition, it is possible to overcome the disadvantage that remarkable physiological activity is not exhibited due to the transdermal application due to the characteristics of the hydrophilic polymer, and the flavonoid content in the form of the non-glycoside, which is a physiologically active ingredient of the fermented herbal medicine extract, I know that I can.

In order to achieve the above object, the inventors of the present invention have confirmed that an increase in the content of an effective ingredient in a fermented extract of herbal medicines cultured by using omega containing oerocyte and that the fermented extract of herbal medicines does not cause skin irritation, The present invention has been accomplished by confirming that the composition has excellent effects.

The present invention provides a method for producing a herbal medicine, comprising the steps of: obtaining a herbal medicine extract selected from at least one group selected from the group consisting of a root chamber, a windblown cloth, a gardenia fruit, a rhizome, a rhizome seedling, a rhus vernacolor, a briar ripened seed, Culturing the mixture of the herbal medicine extract with a pre-culture medium of mycelia of leaf mushroom or mycelia of leaf mushroom, removing the mushroom mycelium from the present culture medium to obtain a herbal extract; Mixing and culturing the crude herbal extract with the Jeju Ome stock; The present invention also provides a composition for external application for skin, which comprises, as an active ingredient, a complex fermented extract of herbal medicines obtained through the step of removing myrrh into a host culture medium.

Hereinafter, the present invention will be described in detail.

In the case of an extract obtained by simple extraction from a herbal medicine selected from at least one group selected from the group consisting of a rootstock, a windbreak, a gardenia fruit, a wilt, a rhizome seedling, a rhus vernacolor, a briar mature seed, a briar ripening seedling and a scrubber, However, when it is applied to an external preparation for skin, there is a problem that the effect is not substantially satisfactory.

In addition, in the case of the herbal medicine fermented extract obtained by cultivating the mycelium of the herbal medicine from the herbal medicine extract, it is difficult to use it as a cosmetic due to the high viscosity water-soluble polysaccharide, and it is difficult to transdermally pass therethrough, But it is difficult to obtain through the use of transdermal application, which is a form of use of cosmetics.

Accordingly, the present invention relates to a composition for external application for skin which is easy to penetrate through the skin and plays an effective role as an external preparation for skin. It is an object of the present invention to provide a composition for external application for skin, Obtaining a herbal medicine extract which is at least one selected from the group consisting of a herbal medicine and a scarlet; Culturing the mixture with a pre-culture solution of mycelia of leaf mushroom or mycelia of leaf mushroom, removing the mushroom mycelium from the present culture to obtain a fermented extract of herbal medicine; Mixing and culturing the obtained crude herbal extract with the Jeju omega parent to obtain a culture solution; And a step of obtaining a secondary filtrate by removing the Mycelia from the host culture medium of Oume. The combined fermentation extract of herbal medicines obtained from the above step contains the herbal medicinal fermentation extract obtained from the one or more herbal medicines The total phenolic content and total flavonoid content were increased and the polysaccharide of the polysaccharide was found to be low molecular weight.

There is no particular limitation on the mixing ratio of the herbal medicines. Preferably, the mixing ratio of the herbal medicines is 1 to 30 parts by weight, preferably 1 to 30 parts by weight, more preferably 1 to 30 parts by weight, .

The combined herbal composition fermentation extract finally obtained in the present invention is preferably contained in an amount of 0.001 to 30.0% by weight based on the total amount of the composition for external application for skin. When the content is less than 0.001% by weight, the skin improving effect is insignificant, This is because it is not economical because it is less efficient than material input.

In the present invention, the extract of the herb medicine is preferably obtained by extraction under reduced pressure or lyophilization, and the extract obtained by the concentration under reduced pressure or lyophilized is most preferably selected from the group consisting of purified water, ethanol, butylene glycol and propylene glycol And preferably 0.001 to 30.0% by weight of a solvent dissolved in at least one solvent selected from purified water, ethanol, butylene glycol and propylene glycol.

In the present invention, the cultivation of mycelia of leaf mushroom (KCTC 10337BP) is preferably carried out by adjusting the pH of the culture medium to 5.0 to 6.0, inoculating the mycelia of leaf mushroom to 4 to 6% (v / v) It is preferable to perform the culture for 5 to 7 days under the conditions of a revolution of 120 to 180 rpm and aeration rate of 1.2 to 1.8 vvm.

In the present invention, the host culture of Jeju Ome is preferably carried out by inoculating the fermented herbal medicine extract to a 1 to 50% (v / v) stock of Jeju Omega, and then cultured at 25 to 37 캜, at a rotation speed of 120 to 180 rpm, vm for 3 to 6 days. The cultivation after the inoculation of the microorganisms can be carried out at a temperature of 25 to 37 캜 in consideration of the microorganisms to be inoculated. If the incubation temperature is lower than the above range, the fermentation rate may be slowed and undesired fermentation products may be produced. Also, if the incubation temperature is higher than the above range, the fermentation rate may be slowed down or the undesirable fermentation products It can happen. In the case of the incubation time, it is preferable to continue until the growth of the inoculated microorganism reaches a maximum. The point at which the growth of the inoculated microorganism reaches its maximum will depend on the incubation temperature, the amount of fermentation material, the amount of microorganism inoculated, and so on. It is possible to determine at what point in time the maximum growth of the inoculated microorganism takes place in consideration of the culture temperature, the amount of the fermentation raw material, the amount of the microorganism to be inoculated, and the like within the normal capability range.

Means that the secondary filtrate is added to the composition to such an extent as to exhibit an improvement effect on the skin condition from the cosmetic composition of the present invention. The term " included as an active ingredient " It is meant to include formulating into various forms by adding various components as subcomponents.

The present invention also relates to a method for inhibiting the activity of a metalloproteinase (MMP-1), a method of inhibiting the activity of a substrate metalloproteinase (MMP-1 Inhibitory effect on inflammation-induced hyaluronidase activity, cytotoxic relaxation effect on ultraviolet irradiation, inhibitory effect on inflammatory cytokine expression by ultraviolet irradiation, An antimicrobial effect against acne bacteria, an anti-inflammatory effect against atopic dermatitis, and an excellent water hygroscopicity.

Further, the present invention relates to a cosmetic composition characterized by using an aqueous solution containing 85 to 99% (v / v) of distilled water as an extraction solvent for extracting the herbal medicines.

The present invention also relates to a cosmetic composition, wherein the cosmetic composition is a cosmetic preparation, a gel, a water-soluble liquid, a cream, an essence, an oil-in-water (O / W) type and a water-in-oil (W / O) type; Wherein the composition is a cosmetic composition selected from the group consisting of a makeup base, a foundation, a skin cover, a lipstick, a lip gloss, a face powder, a two-way cake, an eye shadow, a cheek color and an eyebrow pencil.

The present invention also relates to a cosmetic composition exhibiting a skin whitening effect containing the herbal composition complex fermentation extract.

The present invention also relates to an anti-aging functional cosmetic composition containing the above-mentioned complex herbal extract.

The present invention also relates to a cosmetic composition showing skin irritation alleviating effect containing a complex herbal composition fermented extract.

The present invention also relates to a cosmetic composition exhibiting an atopic skin improving effect containing a complex herbal composition fermented extract.

In addition, the present invention relates to a cosmetic composition exhibiting an acne-preventing effect containing a complex herbal composition fermented extract.

The fermented extract of herbal medicines according to the present invention exhibited a whitening effect, such as melanin production inhibitory effect, which is a cause of spots, freckles and skin pigment deposition, which is superior to conventional simple extracts even without skin irritation.

The fermented extract of herbal medicines according to the present invention exhibited an excellent anti-aging effect, such as an active oxygen scavenging effect, an MMP inhibitory effect, and an effect of regulating MMP expression by ultraviolet irradiation, and relieves skin irritation caused by ultraviolet irradiation.

In addition, the complex herbal extract of fermented herbs according to the present invention showed an effect of alleviating skin irritation and improving atopy by inflammatory mediators.

Therefore, cosmetic compositions such as lotion, cream, emulsion, pack, powder and the like containing the herbal composition complex fermentation extract have the effects of active oxygen scavenging effect and collagenolytic enzyme activity regulating effect, skin wrinkle improving effect, whitening, moisturizing, And exhibits excellent skin external agent effect such as stimulation relaxation effect.

Hereinafter, the present invention will be described in more detail by way of examples. It should be noted, however, that these examples are only illustrative examples of the present invention, and the scope of the present invention is not limited to these examples.

Comparative Example  One: From herbal medicines  Extract preparation

95% (v / v) of 30 grams of shrubs, 30 grams of windbreaks, 30 grams of garden radishes, 30 grams of worms, 30 grams of rhusi matured, 30 grams of rhus mature, 30 grams of immature bryophytes, 30 grams of briar, The mixture was refluxed three times for 5 hours with distilled water, cooled down, and filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 DEG C or lower, and the lyophilized product thus obtained was dissolved in a mixed solvent of purified water, ethanol, butylene glycol and propylene glycol so as to contain 15 wt% of the lyophilized product to prepare a herbal medicine extract.

Comparative Example  2: Comparative Example  1 of Herbal medicine  By fermenting the extract Herbal medicine  Fermented extract preparation

The mycelium of mushroom KCTC 10337BP was cultured in a test tube containing yeast-malt-agar agar medium, stored at 4 ° C, and subcultured every month. The mycelia subcultured on the slope medium were aseptically homogenized to prepare an optimized synthetic medium (see Korean Patent No. 10-0461960) in which the herbal medicine mixed extract obtained in Comparative Example 1 and the sugar were mixed, and adjusted to pH 5.5 The cells were inoculated in a liquid culture medium to a concentration of 5% (v / v) of mycelia of leaf mushroom and then cultured for 6 days in a fermenter at a temperature of 27 ° C, a revolution of 150 rpm, and aeration of 1.5 vvm. After the cultivation, the mycelia of the leaf mushroom was removed from the culture solution, and a fermented extract of the herbal medicine was obtained.

Example  One: Comparative Example  2 Herbal medicine  The fermented extract is subjected to a secondary fermentation Herbal medicine Manufacture of recombinant fermented extract

Jeju Island Traditional Omechinju, which has excellent activity of Mycobacterium tuberculosis or glycosidase, was supplied immediately after production and stored at 4 ℃ and used within 5 days. The fermented herbal medicine extract obtained in Comparative Example 2 was inoculated with 10% (v / v) of Jeju Ome base, and then cultured for 5 days in a fermenter at a temperature of 32 캜, a revolution of 150 rpm and a ventilation rate of 1.5 vvm. After the cultivation, the Mycelium was removed from the culture solution, and a complex herbal extract was obtained.

Experimental Example  1: Determination of components and content in extracts

Qualitative analysis of phenolic materials, flavonoids, flavonoid glycosides and the like among the main components of the herbal medicine extract of Comparative Example 1, the herbal medicine fermentation extract prepared in Comparative Example 2, and the combined herbal medicine fermentation extract prepared in Example 1, Total flavonoid contents were compared.

First, qualitative analysis of phenolic materials, flavonoids, flavonoid glycosides and the like was carried out as follows.

(1) Qualitative analysis of phenolic substances and flavonoids

When the extracts of Comparative Example 1, Comparative Example 2 and Example 1 were left to stand with 1 to 2 drops of a 2.5% FeCl ₃ ethanol solution, they were colored in dark green or observed for the formation of precipitates.

(2) Qualitative analysis of flavonoid glycosides

Using concentrated sulfuric acid method, 5 ml of concentrated sulfuric acid was added to samples of Comparative Example 1, Comparative Example 2 and Example 1, respectively, to observe the formation of yellow and yellow red precipitates.

(3) Qualitative analysis of sugar

After adding 2-3 drops of 5% a-naphthol alcohol TS to each of the samples of Comparative Example 1, Comparative Example 2 and Example 1, red violet appeared on the interface when concentrated sulfuric acid was added through the air barrier.

As a result of the qualitative analysis, it was confirmed that the samples of Comparative Example 1, Comparative Example 2 and Example 1 contain a phenolic substance and a flavonoid flavonoid glycoside.

Total phenol content and total flavonoid content were measured as follows.

(1) Total phenol content measurement

10 ml of distilled water was added to each of the samples of Comparative Example 1, Comparative Example 2, and Example 1, followed by addition of 2 ml of Folin-Ciocalteu phenol reagent (Sigma), followed by reaction at room temperature for 5 minutes. 2 ml of 20% sodium carbonate was added to the reaction mixture, and the mixture was reacted at room temperature for 1 hour and then absorbance was measured at 680 nm. At this time, gallic acid was used as the indicator material.

(2) Total flavonoid content measurement

1.5 ml of the samples of Comparative Example 1, Comparative Example 2 and Example 1 were mixed with 2% AlCl ₃ .H ₂ O dissolved in the same amount of methanol, and reacted at room temperature for 10 minutes, and the absorbance was measured at 367 nm. At this time, the indicator material was catechin.

Name of sample Total phenol content
(μg / mg of extract) Total flavonoid content
(μg / mg of extract) Comparative Example 1 580.5 21.2 Comparative Example 2 615.1 30.9 Example 1 912.5 58.7

The total phenol content and the total flavonoid content of the sample of Example 1 were higher than those of Comparative Example 1 and Comparative Example 2 (Table 1), and the phenol content was about 1.5 The content of pear and flavonoid increased about 2 times.

From the above results, it was confirmed that the total phenol content and the total flavonoid content can be increased by fermenting Jeju Ome stock with the filtrate obtained by fermenting the herbal medicine extract and the mycelia of leaf mushroom prepared by simple extraction, And the extract of the present invention.

Experimental Example  2: Free radical  Measurement of scavenging activity

In order to measure the free radical scavenging activity of the sample obtained in Example 1, free radical scavenging activity was measured using a DPPH method using an extract having excellent antioxidative activity such as green tea extract under laboratory conditions as a comparative sample.

The DPPH method measures free radical scavenging activity by reducing power using a free radical called DPPH (2,2-Di (4-tert-octylphenyl) -1-1picrylhydrazyl free radical). Free radical scavenging was measured at a wavelength of 560 nm by comparing the degree of reduction of the absorbance by DPPH reduction by the test substance with the absorbance of the blank test solution.

For the measurement of DPPH free radical scavenging activity, 0.2%, 0.1%, 0.05% and 0.005% concentration of high temperature and high pressure extracts were prepared. The extracts of the above concentrations were placed in a 96-well plate, and DPPH prepared from 100 uM methanol solution was added thereto to make the total volume of the solution 200 μl. After incubation at 37 ° C for 30 minutes, absorbance was measured at 560 nm.

&Quot; (1) "

Free radical scavenging activity (%) = {100- (B / A)} X100

A: Absorbance of the control well without treatment of the sample of the present invention,

B: absorbance of the experimental group well treated with the sample of the present invention,

As shown in Table 2, the fermented herbal composition fermentation extract of Example 1 exhibits more excellent antioxidative effect than the green tea extract having excellent antioxidant activity.

Name of sample Antioxidative effect(%) Comparative Example 1 (0.1% by weight) 85 Comparative Example 2 (0.1% by weight) 92 Example 1 (0.1% by weight) 98 Green tea extract 92

Experimental Example  3: Cytotoxicity of the sample

The cytotoxicity of the combined herbal composition fermentation extract obtained in Example 1 on skin cells was evaluated. Fibroblast diluted in a cell culture medium (DMEM supplemented with 10% FBS) was added to each well of a 96-well test plate at 1 × 10 5 cells / mL for 24 hours. The sample obtained in Example 1 was diluted to an appropriate concentration in each well and treated for 24 hours. After 24 hours, the medium was removed, and 200 μl of a cell culture medium containing MTT (3- (4,5-dimethylthiazol-2yl) -2,5-diphenyl tetrazolium bromide) solution (2.5 mg / And incubated in a 37 ° C CO2 incubator for 2 hours. The medium was removed and 100 μl of DMSO (dimethyl sulfoxide) was added. After shaking for 5 minutes to dissolve the cells, absorbance at 565 nm was measured in a microplate reader. The cell viability (%) was determined as in the mathematics 2 and the concentration of the sample that did not affect cell survival was determined.

&Quot; (2) "

Cell survival rate (%) = {(St-Bo) / (Bt-Bo)} X 100

Bo: 565 nm absorbance value of wells reacting with cell culture medium,

Bt: 565 nm absorbance value of a well color-reacted without sample treatment,

St: 565 nm absorbance of the well treated and chromogenic.

Name of sample 100% cell viability concentration Comparative Example 1 (0.1% by weight) 2% Comparative Example 2 (0.1% by weight) 2% Example 1 (0.1% by weight) 2%

As shown in Table 3, when the concentration of the skin fibroblast treated at a concentration of 0.1% by weight in the case of Comparative Example 1, Comparative Example 2 and Example 1 was less than 2%, it was generally used in cosmetics It is a safe sample with low possibility of skin irritation when concentration is considered.

Experimental Example  4: In bito MMP -1 activity inhibition experiment

Fluorescence assay was used to measure the inhibitory effect of MMP-1. The substrate used was DQ-Collagen labeled with fluorescein and the collagenase was purchased from Molecular probe (Eugene, OR, USA) and incubated in reaction buffer (0.5 M Tris-HCl, 1.5 M NaCl , 50 mM CaCl2, 2 mM sodium azide, pH 7.6) was used after 10-fold dilution. 20 coll of DQ collagen dissolved in a reaction buffer at 0.25 mg / ml in the reaction buffer, 40 비교 of the samples obtained in Comparative Examples 1 and 2 and Example 1 (0.1% by weight) were added, and the collagenase diluted with 0.5 units (collagenase). After 20 minutes at room temperature in a dark place, the fluorescence value was measured using a fluorescence spectrophotometer (Perkin Elmer, UK) with an absorption wavelength of 495 nm and an emission wavelength of 515 nm. As a control, a fluorescence value The fluorescence value of the sample itself was also measured and corrected for the enzyme activity.

The results are shown in Table 4. The metal protease (MMP-1) inhibitory activity was excellent when 0.25% of the sample of Example 1 was treated, and the inhibitory activity was better than that of green tea extract.

From the above results, it can be confirmed that the combined fermented herbal extract extract has excellent inhibitory effect on metalloproteinase (MMP-1) activity. Based on this fact, the composition for external application for skin of the present invention effectively inhibits metalloproteinase, It is possible to deduce that it is possible to prevent or improve wrinkles and wrinkles.

Name of sample MMP-1 activity inhibition rate (%) Comparative Example 1 (0.25% by weight) 88 Comparative Example 2 (0.25% by weight) 70 Example 1 (0.25% by weight) 51 Green tea extract 0.25 wt% 67

Experimental Example  5: UV irradiation Herbal medicine  By complex fermentation extract MMP -1 expression inhibition evaluation

Enzyme immunoassay (ELISA) was performed to measure the concentration of MMP-1 after addition of the sample obtained in Example 1 after UV irradiation.

UVA was irradiated in human dermal fibroblasts at an energy of 6.3 J / cm < 2 > using a UV chamber. Ultraviolet irradiation dose and incubation time were established by preliminary experiment to maximize MMP expression level in fibroblasts. The negative control was wrapped in a silver foil and the cells in the UVA environment were transferred to the medium containing the sample after irradiated with UVA, and cultured for 24 hours. Then, the medium was recovered and coated on 96 wells. The primary antibody (MMP-1 (Ab-5) monoclonal antibody) was treated and reacted at 37 ° C for 60 minutes. The cells were incubated with a secondary antibody, anti-mouse IgG (alkaline phosphatase conjugated), for 60 minutes, and incubated with an alkaline phosphatase substrate solution (diethanolamine buffer solution containing 1 mg / ml p-nitrophenyl phosphate) The reaction was carried out at room temperature for 30 minutes and the absorbance at 405 nm was measured with a microplate reader. As a control group, a sample to which no sample was added was used.

As shown in Table 5, when the sample of Example 1 was added to MMP-1 induced by UV irradiation, the inhibition rate of MMP-1 was increased and the inhibition rate was 65% or more as compared with the control without the sample. This is an excellent result compared to the inhibition rate of retinol used as a control.

Test group MMP-1 expression inhibition rate (%) Negative control group - Comparative Example 1 (0.25% by weight) 73 Comparative Example 2 (0.25% by weight) 60 Example 1 (0.25% by weight) 31 Retinol 41

Experimental Example  6: Tyrosinase  Activity inhibition experiment

In order to confirm the tyrosinase inhibitory effect of the sample obtained in Example 1, arbutin, well known as a whitening agent, was used as a comparative sample. Tyrosinase was isolated and purified from mushrooms and purchased from Sigma. L-Tyrosine (Sigma), a substrate, was dissolved in 0.05 M sodium phosphate buffer solution (pH 6.8) and used as a 0.1 mg / ml solution. The sample of Example 1 was dissolved in 0.05 M sodium phosphate buffer solution (pH 6.8) adjusted to a suitable concentration and then used. 0.5 ml of L-tyrosine solution was placed in a test tube, 0.5 ml of a sample was added thereto, and the mixture was reacted at 37 占 폚 for 25 minutes. The test tube containing the reaction solution was placed on ice, quenched to stop the reaction, and the absorbance at a wavelength of 475 nm was measured. As a control group, 0.5 ml of buffer solution was used instead of the above-mentioned sample.

The inhibition rate of tyrosinase activity of each of the above samples was calculated according to Equation (3).

&Quot; (3) "

Tyrosinase activity inhibition rate (%) = 100 - [(comparative group absorbance / control group absorbance)] X100

The results are shown in Table 6, and the inhibition rate of tyrosinase in the sample treatment of Example 1 is similar to that of arbutin.

Name of sample Inhibition rate of tyrosinase activity (%) Control group - Comparative Example 1 (0.25% by weight) 77 Comparative Example 2 (0.25% by weight) 68 Example 1 (0.25% by weight) 40 Arbutin 0.25 wt% 59

Experimental Example  7: inhibition of melanin synthesis by B16F1 melanocytes

Comparative Example 1, Comparative Example 2 and Example 1 To confirm the whitening effect of the sample, the effect of inhibiting melanin synthesis on B16F1 melanocytes was measured.

Melanin synthesis inhibitory effect of B16F1 melanocytes was measured as follows. B16F1 melanocytes were plated at a concentration of 2 x 10 ⁶ per well in a 6-well plate, and after attaching the cells, the samples were treated at a concentration not causing toxicity and cultured for 72 hours. After incubation for 72 hours, the cells were detached with trypsin-EDTA, and the number of cells was measured, followed by centrifugation to recover the cells. Quantification of intracellular melanin was performed using lotan: Cancer Res. , 40, 3345-3350 (1980)]. The cell pellet was washed once with PBS, and 1 ml of homogenization buffer (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF (phenylmethylsulfonyl fluoride)) was added and vortexed for 5 minutes to disrupt the cells. After centrifugation (3,00 rpm, 10 min) gkdu, 1N NaOH (10% DMSO (Dimethyl Sulfoxide)) was added to the cell filtrate to dissolve the extracted melanin and the absorbance of melanin was measured at 405 nm with a microplate reader. Was measured to determine the inhibition rate (%) of melanin formation in the sample. The inhibition rate (%) of melanin formation of B16F1 melanocytes was calculated by the following equation (4).

&Quot; (4) "

Melamine synthesis inhibition rate (%) = [(A-B) / A] x 100

A: Amount of melanin in wells to which no sample was added

B: Amount of melanin in the well to which the sample was added

As shown in Table 7, the melanin synthesis inhibitory effect of B16F1 melanocytes was examined. As a result, the melanin synthesis inhibitory effect of the herbal medicinal fermentation extract sample of Example 1 was inhibited from 0.25% to 47%. In particular, the melanin synthesis inhibitory effect was superior to that of the herbal medicine fermented extract of Comparative Example 2, and the melanin synthesis inhibitory effect similar to arbutin was exhibited.

Name of sample Melanin synthesis inhibition (%) Negative control group - Comparative Example 1 (0.25% by weight) 20 Comparative Example 2 (0.25% by weight) 33 Example 1 (0.25% by weight) 57 Arbutin 0.25 wt% 44

Experimental Example  8: Cytotoxicity mitigation effect by ultraviolet irradiation

Comparative Example 1, Comparative Example 2 and Example 1 The mitigation effect of cytotoxicity by ultraviolet irradiation of the sample was evaluated. Fibroblasts were placed in 24-well test plates at 1 × 10 ⁵ cells and adhered for 24 hours. Each well was washed once with PBS and 500 ul of PBS was added to each well. The cells were irradiated with 10 mJ / cm 2 of ultraviolet light using ultraviolet B (UVB) lamp (Model: F15T8, UVB 15W, Sankyo Dennki, Japan) 1 ml) was added. Here, the sample to be evaluated was treated and cultured for 24 hours. After 24 hours, the medium was removed, and cell culture medium (500 μl) and MTT solution (2.5 mg / ml) (60 μl) were added to each well and incubated for 2 hours at 37 ° C in a CO2 incubator. The medium was removed and 500 μl of isopropanol-HCl (0.04 N) was added. The cells were shaken for 5 minutes to dissolve the cells, and 100 쨉 l of the supernatant was transferred to a 96-well test plate, and absorbance at 565 nm was measured in a microplate reader. The cell viability (%) was measured as shown in Equation (5) and the cytotoxic mitigation rate by ultraviolet was calculated by Equation (6).

Equation (5)

Cell survival rate (%) = {(St-Bo) / (Bt-Bo)} X 100

Bo: absorbance at 565 nm of the well in which only the cell culture medium was color-

Bt: absorbance at 565 nm of a well that underwent chromogenic reaction in a well not treated with the sample,

St: Absorbance at 565 nm of a well that underwent chromogenic reaction in a sample-treated well.

&Quot; (6) "

Cytotoxicity mitigation rate by ultraviolet ray (%) = 1- (St-Bo) / (Bt-Bo) X 100

Bo: the cell survival rate of wells not irradiated with ultraviolet rays and not treated,

Bt: cell survival rate of wells irradiated with ultraviolet rays and not treated with samples,

St: Cell survival rate of well irradiated and treated samples.

As shown in Table 8, the result of the experiment showed that the herbal medicinal fermented extract of Example 1 showed cytotoxicity by ultraviolet light of 60% at the concentration of 0.25%. It was confirmed that the cytotoxic relaxation was increased as compared with the sample of Comparative Example 1, and it was found that the cytotoxicity by ultraviolet rays was effectively prevented even at a low concentration.

Name of sample Cytotoxic Relaxation Rate (%) Comparative Example 1 (0.25% by weight) 35 Comparative Example 2 (0.25% by weight) 47 Example 1 (0.25% by weight) 80

Experimental Example  9: Inhibitory effect of inflammatory cytokine expression by ultraviolet irradiation

Comparative Example 1, Comparative Example 2 and Example 1 in order to evaluate the expression of inflammatory cytokine inhibitory effect that is expressed by the ultraviolet ray irradiation of the sample 5X10 fibroblasts (Fibroblast) isolated from the skin tissue of the person in a 24-well test plate ⁴ Were added and allowed to attach for 24 hours. Each well was washed once with PBS and 500 占 퐇 of PBS was added to each well. After irradiating the fibroblasts with 10 mJ / cm 2 of ultraviolet light using an ultraviolet B (UVB) lamp (Model: F15T8, UVB 15W, Sankyo Dennki, Japan), PBS was removed and the cells were cultured in DMEM Medium) was added. Here, the sample to be evaluated was treated and incubated for 5 hours. 150 [mu] l of culture supernatant was taken to quantify IL-1 [alpha], and the effect of inhibiting the expression of inflammatory cytokines was determined. The amount of IL-l [alpha] was quantitated using Enzyme-linked Immunosorbent Assay and the production rate of IL-l [alpha] was calculated by Equation (7).

&Quot; (7) "

Inhibitory rate of inflammatory cytokine expression (%) = 1- (St-Bo) / (Bt-Bo) X 100

Bo: IL-1? Production amount in wells not subjected to ultraviolet irradiation,

Bt: the amount of IL-1? Production in wells irradiated with ultraviolet light and not treated with the sample,

St: IL-1α production in wells irradiated with ultraviolet light and treated with the sample.

As a result of the experiment, it can be seen that the combined herbal composition fermentation extract of Example 1 inhibits the production of IL-1α, which is an inflammatory cytokine caused by ultraviolet rays, by 47% at a concentration of 0.25%, and effectively protects the generation of inflammation caused by ultraviolet rays at low concentrations (Table 9).

Name of sample Inhibitory rate of inflammatory cytokine expression (%) Comparative Example 1 (0.25% by weight) 25 Comparative Example 2 (0.25% by weight) 40 Example 1 (0.25% by weight) 67

Experimental Example  10: promoting type 1 procollagen biosynthesis

Procollagen assay was conducted as follows to examine the effect of accelerating the biosynthesis of type 1 procollagen, which is a skin matrix component, after adding the herbal composition complex fermentation extract obtained in Example 1.

Human dermal fibroblasts isolated from neonatal foreskin tissues were purchased from Modern Tissue Technology (MTT, Korea) and supplemented with 10% fetal bovine serum (FBS) in DMEM / F-12 (3: And cultured at a concentration of 1 × 10 ⁴ cells / cm 2. When 70-80% of the cells were grown, the cells were subcultured at a ratio of 1: 3, and cells in the third to fourth subculture were used for the experiment. For the measurement of the amount of procollagen, the fibroblasts were cultured in a 48-well plate at 90% or more, the sample of Example 1 was added at a concentration of 0.25%, and the amount of procollagen liberated in the medium after 24 hours 1 procollagen type-1 C-peptide EIA kit (MK101, Takara, Japan).

The results are shown in Table 10. The treatment of 0.25% of the sample of Example 1 promoted 64% of the biosynthesis of procollagen, and the promotion of the biosynthesis of procollagen was more than twice as much as that of Comparative Example 1, Which is similar to that of TGF-β.

Name of sample Promoting effect of procollagen biosynthesis Comparative Example 1 (0.25% by weight) 25 Comparative Example 2 (0.25% by weight) 40 Example 1 (0.25% by weight) 64 TGF-beta 50

Experimental Example  11

A cosmetic composition containing the herbal composition complex fermentation extract obtained in Example 1 was prepared and compared with Comparative Example 3 to humans to evaluate skin elasticity improving effect and wrinkle improving effect.

The cosmetics used in the comparative experiments are in cream form and their composition is shown in Table 10. First, the b) image recorded in Table 11 is heated and stored at 70 ° C. A) was added to the mixture to pre-emulsify the mixture, homogeneously emulsified with a homomixer, and then slowly cooled to prepare a cream (Example 2, Comparative Example 3). Twenty patients (20 to 35 years old) were applied with the cream prepared in Example 2 on the right side of the face and the cream prepared in Comparative Example 3 on the left side of the face for 2 consecutive months, respectively .

The skin elasticity was measured by using a skin elasticity meter (SEM 575, C + K Electronic Co., Germany) before and after using the product for 2 months. The experimental results are shown in Table 12 below as ΔR7 values of Cutometer SEM 575, where R7 values represent the viscoelasticity of the skin. As shown in Table 12, it can be seen that the skin elasticity improvement effect of the creamer-coated cream containing the herbal medicinal fermented extract is excellent.

Raw material Example 2 Comparative Example 3 Stearyl alcohol 8 8 Stearic acid 2 2 Stearic acid cholesterol 2 2 Squalane 4 4 2-octyldodecyl alcohol 6 6 Polyoxyethylene (25 molar parts
A) Alcohol ester 3 3 Glyceryl monostearate
Aster 2 2 The herbal medicinal fermented extract of Example 1 One - Propylene glycol 5 5 Suitable amount Suitable amount Suitable amount

Note) Unit: wt%

Experimental product Skin elasticity effect (△ R7) Example 2 0.38 Comparative Example 3 0.11

n = 20, p < 0.05

Twenty patients (20 to 35 years old) were applied with the cream prepared in Example 2 on the right side of the face and the cream prepared in Comparative Example 3 on the left side of the face for 2 consecutive months, respectively .

To evaluate the wrinkle improvement effect of the skin before and after the use of the product after the completion of the experiment, a silicone replica was made and the wrinkle state of the designated area was measured with a visiometer SV60, C + K Electronic Co., Germany). The results are shown in Table 13, which shows the average of the parameter values after 2 months minus the parameter values two months ago. That is, the negative value indicates that the wrinkle improving effect is higher. Therefore, as shown in Table 13, it was confirmed that the effect of improving the wrinkles of the skin of Example 2 containing the mixed herbal composition fermentation extract of Example 1 was greatly improved.

Experimental product R1 R2 R3 R4 R5 Example 2 -0.21 -0.17 -0.11 -0.08 -0.09 Comparative Example 3 -0.12 -0.06 -0.05 -0.04 -0.02 R1: Difference between the maximum value and the minimum value of the wrinkle contour line
R2: The wrinkle contour line is arbitrarily divided into 5 squares, and the average of R1 values
R3: Maximum value of R1 divided by 5
R4: Mean value of the baseline of the wrinkle contour minus the value of each apex and valley
R5: Mean value of the value obtained by subtracting the outline of each wrinkle from the base line of the wrinkle contour line

Experimental Example  12

A cosmetic preparation containing the herbal composition complex fermentation extract obtained in Example 1 was prepared and skin whitening effect was evaluated in humans by performing a comparative experiment with Comparative Example 3.

The cosmetics used in the comparative experiment are in the form of a cream, and the composition thereof is shown in Table 11. First, the b) image recorded in Table 11 is heated and stored at 70 ° C. A) was added to the mixture to pre-emulsify the mixture, homogeneously emulsified with a homomixer, and then slowly cooled to prepare a cream (Example 2, Comparative Example 3). Twenty patients (20 to 35 years old) were applied with the cream prepared in Example 2 on the right side of the face and the cream prepared in Comparative Example 3 on the left side of the face for 2 consecutive months, respectively . After completion of the test, the skin of the applied area on both right and left sides of the face was analyzed by image analyzer and color change (ΔL) of the color was measured using a Minolta CR300, and an objective visual observation by a plurality of expert Subjective visual observation was performed and the effect was measured according to the following classification. The results are shown in Table 15 below. At this time, the degree of whitening efficacy was classified into the following seven grades and evaluated.

Whitening efficacy evaluation criteria; -3: very bad, -2: worse, -1: slightly worse, 0: no change, 1: slight improvement, 2: improvement, 3:

As shown in Table 14, it can be seen that the whitening effect is excellent in the facial skin of an experimenter who applied the cream containing the herbal medicine complex fermented extract.

Change in skin color brightness (? L) Objective evaluation of expert Subjective evaluation of the subject Experimental Example 11 Comparative Example 4 Experimental Example 11 Comparative Example 4 Experimental Example 11 Comparative Example 4 3.98 1.44 2.77 1.55 3.07 1.7

Experimental Example  13: SLS Effect of skin irritation mitigation

In this experiment, the effect of relaxing the stimulants of the cosmetic composition containing the herbal medicinal fermented extract obtained in Example 1 was evaluated by a human skin patch test.

1% of sodium lauryl sulfate (SLS), which stimulates general cosmetic prescriptions (cream, lotion, skin, essence), and the product manufactured in Example 2 were mixed with each other for 24 hours, 48 hours and 72 hours, And the effect of stimulation relaxation was evaluated.

Each product was sprayed with 0.3 mg of FINN CHAMBER (FINLAND) on the upper arm of 50 healthy men and women aged 20 to 50 years, and the acute irritation index was evaluated after 24 hours. After the evaluation, the same amount of the product was applied again to the same site again to evaluate the delayed irritation index after 48 hours and 72 hours.

As a result of the test, when the SLS was applied alone, irritation appeared on the skin after 24 hours, but when the product containing the complex herbal extract of fermented product of the present invention was applied, no skin after 24 hours, 48 hours, It did not cause seizures.

This result shows that there is a significant effect of reducing the skin irritation caused by the stimulant substrate (surfactant, fragrance, alcohol) when the complex herbal extract of the present invention is mixed with cosmetics.

Other examples are shown below. In other words, lotion, milky lotion and serum containing the fermented herbal extract of the present invention obtained in Example 1 were prepared as in Examples 3 to 5. The cosmetic lotion, emulsion and cosmetic liquid containing the herbal composition complex fermented extract of the present invention can improve the skin such as antioxidative effect, collagen synthesis promoting effect, skin wrinkle improving effect, whitening effect, moisturizing effect, skin irritation mitigation effect, atopy improvement effect and anti- .

Example  3: Herbal medicine  Manufacture of lotion containing complex fermented extract

0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of perfume, 0.1 g of p-hydroxybenzoic acid methyl ester, a small amount of antioxidant and a small amount of dye were mixed in 8 g of 95% ethanol. 10 g of the herbal composition complex fermentation extract of the present invention obtained in Example 1 and 5 g of glycerin were dissolved in 85.33 g of purified water. The mixture was added to the mixture, followed by stirring to obtain a lotion having skin-improving effect.

Example  4: Herbal medicine  Manufacture of emulsion containing complex fermented extract

0.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of p-hydroxybenzoic acid ethyl ester, 1 g of glycerin monoestearylate, 1 g of polyoxyethylene (20 mol added) monooleate and 0.1 g of coryneform were heated and mixed at 70 캜 0.5 g of the herbal composition fermentation extract of the present invention obtained in Example 1, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1,500, 0.2 g of triethanolamine and 76.2 g of purified water were dissolved by heating at 75 캜. The two were mixed and emulsified and then cooled to obtain an oil-in-water (O / W) type milky lotion.

Example  5: Herbal medicine  Mixed fermented extract Serum  Produce

To 5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of chitoolose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium permanganate, 0.1 g of p-hydroxybenzoic acid ethyl ester 1 g of the herbal composition complex fermented extract of the present invention obtained in Example 1 and an appropriate amount of pigment were mixed to obtain a serum having skin-improving effect.

Claims (9)

(A) extracting herbal medicines including rootstocks, food windbreaks, gardenia fruit, persimmon seeds, rhusi mature seeds, rhus vernacolor, maturing bryophytes, briar ripening, and scouring;
(B) mixing the pre-cultured mycelia of mycelia of leaf mushroom or mycelia of leaf mushroom with the herbal medicine extract to obtain a fermented extract of herbal medicines by removing the mushroom mycelium from the culture medium;
(C) mixing the crude herbal extract with the crude omega parent to obtain a secondary culture; And
(D) removing the Mycelia from the secondary culture to obtain a complex fermented extract of herbal medicines; and a cosmetic composition containing the herbal composition complex fermented extract as an active ingredient. The method according to claim 1,
The cosmetic composition according to claim 1, wherein the herbal medicine in step (a) is 1 to 30 parts by weight, respectively, in the foliage, the windbreak, the gardenia fruit, the wax, the rhizome seedling, the rhus vernacolor, . The method according to claim 1,
Wherein the herbal composition complex fermentation extract is contained in an amount of 0.001 to 30.0% by weight based on the whole cosmetic composition.
The method according to claim 1,
The cosmetic composition may be formulated into a cosmetic, a gel, a water-soluble liquid, a cream, an essence, an oil-in-water (O / W) type and a water-in-oil (W / O) type; A color cosmetic formulation consisting of an underwater type or a water-in-oil type makeup base, a foundation, a skin cover, a lipstick, a lip gloss, a face powder, a two way cake, an eyeliner, a teak collar and an eyebrow pencil; And the cosmetic composition is a cosmetic composition.
The method according to claim 1,
Wherein the cosmetic composition is for skin whitening. delete The method according to claim 1,
Wherein the cosmetic composition is for alleviating skin irritation. delete delete