KR101195577B1 - Cosmetic composition for alleviating skin inflammation - Google Patents

Abstract

The present invention relates to a cosmetic composition for mitigating skin inflammation, and more particularly, steam or extract agar and blossoms mushrooms belonging to red algae (Sparassis crispa ), Agaricus mushroom blazeii ), containing a mixed extract consisting of Polyporus umbellatus and relates to a cosmetic composition for alleviating skin inflammation, alleviating skin irritation or antioxidant.

Description

Cosmetic composition for alleviating skin inflammation {Cosmetic composition for alleviating skin inflammation}

The present invention relates to a cosmetic composition for mitigating skin inflammation, and more particularly, steam or extract agar and blossoms mushrooms belonging to red algae (Sparassis crispa ), Agaricus mushroom blazeii ), containing a mixed extract consisting of Polyporus umbellatus and relates to a cosmetic composition for alleviating skin inflammation, alleviating skin irritation or antioxidant.

The red algae extract and the mushroom mixture extract of the present invention exhibit dermatitis prevention and improvement effect due to antioxidant effect, NO production inhibitory effect, lipid peroxidation inhibitory effect, anti-inflammatory effect and the like.

Antioxidation refers to the inhibition of various oxidation reactions in the body. Oxidation refers to a phenomenon in which various unsaturated fatty acids present in the body are oxidized to form lipid peroxides and aging is accelerated by the increase of these substances. In order to prevent the aging caused by the free radicals formed by the oxidation reaction, SOD (superoxide dismutase), CAT (Catalase), GSHpx (Glutathione peroxidase), etc. exist to protect the body from oxidative stress. .

On the other hand, the inflammatory response is known to have a number of causes, one of which is the cytokine produced by macrophages and NO (nitric oxide) plays an important role. NO is produced by iNOS in various pathological processes such as inflammation and cancer. NOS is produced from L-arginine as an important enzyme that regulates inflammation, vasodilation, neurotransmission, tumor cell and homeostasis.

The skin cells are damaged by such oxidative stress and inflammatory reactions. If the cell proliferation of the damaged skin is not properly performed, the skin is further damaged, and it is also important to actively proliferate the skin cells.

Conventionally, various methods have been tried to alleviate skin irritation, and for example, a method of removing a substance causing skin irritation has been attempted. However, this research is limited because the functional raw material itself often acts as a stimulus source. For example, lactic acid, glycolic acid, salicylcic acid, retinoid, etc., are contained in cosmetics to promote skin cell regeneration or anti-wrinkle effect, but cause skin irritation. do. Accordingly, in recent years, many studies have been conducted to relieve stimulation by neutralizing acidity by adding strong alkalis such as NaOH or KOH to stimulus-inducing functional raw materials, but such a prescription has a problem of lowering the efficacy of existing raw materials. In recent years, studies have been conducted on methods for suppressing intercellular immune-inducing substances, such as neuropeptides such as calcitonin gene-related peptide (CGRP) and substance P (sunstance P), which are secreted from neurons, Various cytokines and PGE2 are involved in edema formation and vasodilation in skin cells and immune cells, such as kinins such as bradykinin, which are activated by stress in tissues or plasma. It is known to act as a cause, and neuropeptide antagonists, bradykinin antagonists, COX inhibitors, etc. have been suggested as substances that can reduce skin inflammation and irritation. Relieve skin irritation caused by ultraviolet rays using a cosmetic composition (Korean Patent Laid-Open Publication No. 2000-0058730), raspberry leaves or heat Skin external composition containing the extract (Republic of Korea Patent Publication No. 2003-0015654 call), a cosmetic composition containing a phytosterol (Patent Publication No. 2002-0063987), etc., but did not indicate a clear effect.

The purpose of this study was to develop natural products with excellent anti-dermatitis prevention and improvement effect due to antioxidant effect, NO production inhibitory effect, lipid peroxidation inhibitory effect, anti-inflammatory effect, and cell proliferation effect.

Therefore, the inventors of the present invention sought to find a natural ingredient having excellent efficacy in protecting the skin from oxidative stress and external harmful substances caused by ultraviolet rays and free radicals. As a result, the red algae extract and the mushroom mixture extract have an excellent effect on relieving skin inflammation. It confirmed that it has a thing, and completed this invention.

Accordingly, an object of the present invention to provide a cosmetic composition for alleviating skin inflammation, including a red ginseng seaweed or wormwood extract and a mixed extract consisting of zinnia mushrooms, mushrooms, mushrooms as an active ingredient.

The present invention also provides a cosmetic composition for reducing skin irritation or antioxidant.

In order to solve the above problems, the present invention may include the following means.

The cosmetic composition for alleviating skin inflammation according to the present invention contains a mixed extract consisting of laver or mantle starch extract and matsutake mushroom, young mushroom and geran.

The cosmetic composition for skin irritation relief according to another embodiment of the present invention contains a mixed extract consisting of laver or wormwood extract and matsutake mushrooms, young mushrooms and nymphs.

The cosmetic composition for skin irritation relief according to another embodiment of the present invention contains a mixed extract consisting of laver or wormwood extract and matsutake mushrooms, young mushrooms, and ghosts.

The content of the mixed extract consisting of the laver or mantle starch extract and the mushroom mushroom, virtuoso mushrooms and aging is characterized in that 0.1 to 20% by weight based on the total weight of the cosmetic composition.

The cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing It is characterized by selecting any one formulation selected from the group consisting of foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder and eye shadow.

The present invention is a seaweed or wormwood extract and blossom mushroom ( Sparassis crispa ), Agaricus mushroom blazeii ), by containing a mixed extract consisting of ( Porporus umbellatus ) as an active ingredient to provide a cosmetic composition to reduce the skin inflammation.

The present invention also provides cosmetics for skin irritation relief and antioxidant.

The cosmetic composition for alleviating skin inflammation or for alleviating skin irritation according to the present invention is a laver or radish extract and Sparassis crispa ), Agaricus mushroom blazeii ), Polyporus umbellatus , as an active ingredient.

The seaweed used in the present invention is a seaweed of the algae of the red algae plant, purple fur, and has the shape of an egg like a long oval or string of 14-25 cm in length and 5-12 cm of butterfly. Types of laver include Porphyra tenera, P.yexoensis, and P. suborbiculata. Among these, laver is the pattern of laver grown in Korea. In particular, laver is rich in calcium and phosphorus, and among them, the polysaccharides contained in laver are known to have physiological effects such as anticancer, antiviral, and anticoagulant effects, as described above.

Gelidium amansii used in the present invention is a seahorse of the red algae plant, distributed in Korea, Japan, Indonesia, etc., inhabits rocks in the middle and bottom of the intertidal zone, the size is 10 to 30 cm, and the butterfly is about 1 mm. to be. Plants are clustered and linear, and are composed of pressed stems and branches that split into idols at both edges, spreading in a fan shape. Locust is usually used as a representative word for plants belonging to the genus, and since it contains agar quality, it is well known as an early seaweed used as the main raw material of agar production. In Korea, there are not only worms, but also larva (Pterocladia tenuis). In particular, wood starfish and ox radish are widely used as raw materials for agar. Agar is made of wood, with 10 ~ 20% mixed with red radish plants such as ox radish, lime, and sack. In addition, stalks and sprouts are also used as agar raw materials, but they are different from each other, so they are usually not mixed together. Locust is a perennial plant whose life history is very regularly repeated between the meteor generation by spouses and the asexual generation by tetraspores. Although there is a slight difference in the growth time depending on the province, it usually grows in May to November. After this period, the mother gradually melts away, leaving only a thin stem of the base. Thus, in spring, a new upright is formed in the remaining base, while the spores grow and thrive together.

The Sparassis crispa ) is a fungus of the Asteraceae. Two species of 1 and 1 genus are reported all over the world. Fruiting body is white flower-shaped cauliflower of 10-25cm in size. The color is generally pale yellow or white, the thickness is about 1mm flat, and the shade is wavy around the surface, and the surface is white to pale yellow. In autumn, pine, pine, larch, beech, buckwheat, etc., root-bearing brown hardwood mushrooms that grow on the ground or on the stump near the roots of conifers or hardwoods. It is one of the rare mushrooms that are rarely found. Blossom mushrooms are distributed in Korea, Japan, China, North America, Europe, Australia, and are edible mushrooms. They are chewable and smell like pine mushrooms, so they are called 'mushroom mushrooms' because they are like flower-shaped mushrooms. In particular, matsutake mushrooms were announced by a research group at the Tokyo University of Pharmacy, and are sold as health supplements. Artificial cultivation of matsutake fruit body production was conducted in spring 1998 by Fukushima Sensei of Kumagaya Agricultural High School, Saitama Prefecture, Japan, and successfully Cultivation is possible. In recent research, it has been explained that beta (1 → 3) D glucan is an ingredient that enhances immunity and prevents cancer. It was found to contain about three times more.

Agaricus blazeii is also known as Agaricus mushroom (Agaricus) or white mushrooms, the folds of the fold fungi mushrooms, the origin is the United States, the distribution region of the United States Florida, Latin America northern highlands, native America Florida Mountainous areas in the states and Brazil, 5 ~ 10cm in sack and 6 ~ 12cm in lampshade, similar in appearance to mushrooms, but thicker and longer in sack. The outer part of the lampshade is white, brown or light brown depending on the development condition, but the sack is white and belongs to the heaviest of the wild mushrooms, the browning of spores is late, and the smell of dry squid or anchovy It has the same sweet smell and chews sweet. First discovered in Florida in the United States in 1944, it became widely known as the indigenous people of Piadade, Brazil's mountainous region, in the mid-1960s and became known for its indigenousity in North America and parts of Latin America. It has not been reported elsewhere. Research into artificial cultivation began around the 1990s when it became known that protein, fat, fiber, vitamins, inorganic salts, amino acids, essential fatty acids, and nucleic acids are rich in nutrients and are good for various diseases such as cancer. As a result, it was succeeded in artificial cultivation for the first time in Japan in 1992, and is now artificially cultivated in Korea and China, and it is difficult to store raw materials because it starts to rot immediately after being taken in its natural state. do.

The age ( Polyporus umbellatus ) is also known as tinnitus, age, age, low age, geodo, rare age, gajeo, etc., and belongs to the fungus fungi democracy fungi, family in the broad-leaved forest in autumn It is parasitic on the roots of alders, oaks, maples and oaks, and its taste is sweet and bitter. It is mainly distributed in Korea, Japan, Europe, China, and the United States, and the sack is divided several times in the base, and the lampshade blooms at the end of each branch. The height is 10 ~ 20cm and the diameter is 10 ~ 30cm. Mycelium is an irregular mass, many similar to ginger, and its surface is peculiar, yellowish white or weasel. It's like a pig's stool, and it's called 'her spirit'. The fruiting body is composed of intricately pruned sacks and lampshades. It is an irregular long lump or a round lump that is uneven in size. The long one is usually curved or split like ginger and is 5 ~ 25cm long. The surface is black or blackish brown or has a humps. Light and durable, cross section milky or yellowish white. Low age is effective for smoothing water metabolism. It is good for urinating or swelling in the whole body. It is good for symptoms of cold and diarrhea due to weak leg and swelling and poor digestion during pregnancy. . Diuretic, antibacterial, anti-tumor, fever, acute urethritis is also used. Effective for late primary lung cancer and liver cancer, and also effective for chronic virulent hepatitis.

The laver or wormwood extract and the matsutake mushroom, young mushroom, and the mixed extract extract water, a polar solvent selected from a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, most preferably, hydrothermally extract by adding water.

In addition, the extract of the seaweed or wormwood extract according to the present invention and the flower mushrooms, mushrooms, mushrooms, and spirits can be prepared as follows.

Laver or bamboo shoots, matsutake mushrooms, mushrooms, and spirits are washed with water and dried in the shade. Dried red algae or blossom mushrooms, mushrooms, mushroom mixtures (1: 1: 1 weight ratio, sweet mushroom mixture extracts can be formed in various composition ratios) are placed in the extraction container, and a predetermined time at an appropriate temperature in an appropriate amount of the extraction solvent Extract. The extract can be filtered and concentrated using a lyophilizer or the like.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof may be used, and preferably water is used.

The amount of the extraction solvent is 2 to 10 times the dry weight of the herbal medicine.

The deposition temperature is 30 ~ 90 ℃, more preferably 60 ~ 80 ℃, most preferably maintained at about 70 ℃ is most effective in the prevention of decay, discoloration and odor.

The immersion time is 5 to 20 hours, preferably 10 hours.

After obtaining the laver or mantle starch extract and matsutake mushroom, spiritual mushroom, mixed mushroom extract, using a filter paper and the like to remove the solids, the suspension is centrifuged, the supernatant may be freeze-dried.

In the composition of the present invention, the mixed extract consisting of the laver or radish larvae extract, matsutake mushroom, young mushroom, and lychee may be contained in an amount of 0.1 to 20% by weight based on the total weight of the cosmetic composition.

The composition of the present invention may contain one or more active ingredients exhibiting the same or similar functions in addition to the laver or mandarin and matsutake mushrooms, mushrooms, mushroom mix extract.

Cosmetics made of a cosmetic composition containing the laver or mandarin and matsutake mushrooms, young mushrooms, and herb mixed extracts of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to cosmetics containing the mixed extract of the present invention, by containing a dermatologically acceptable medium or base, it can be prepared in the form of adjuvants that can be applied topically or systemically as commonly used in the field of dermatology.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to the laver or mandarin and matsutake mushrooms, young mushrooms, age mix extract, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, Foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or hydrophilic It may contain adjuvants commonly used in the field of cosmetics or dermatology, such as oil-based actives, lipid vesicles or any other ingredients commonly used in cosmetics. In addition, the above components may be introduced in an amount generally used in the dermatology field.

Examples of products to which the cosmetic composition of the present invention may be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, and the like, cleansing agents, soaps, treatments, and essences. Etc.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited by the Examples.

Preparation Example 1 Preparation of Laver or Lobster Extract

The laver or wood starfish is washed with water and dried in the shade. 100 g of dried laver or bamboo starch was finely crushed in a crusher, immersed in 500 ml of purified water, and warmed in a 70 ° C. water bath for at least 10 hours. The resulting extract was cooled to room temperature, filtered through a filter paper, and the filtrate was collected to prepare a seaweed extract, laver or laver extract.

Preparation Example 2 Preparation of Blossom Mushroom, Spirit Mushroom, and Mixed Mushroom Extract

The mushrooms, mushrooms, and spirits are washed with water and dried in the shade. 100 g of dried matsutake mushrooms, mushrooms and mushroom mixtures (1: 1: 1) were finely crushed in a crusher, immersed in 500 ml of purified water, and warmed in a 70 ° C. water bath for at least 10 hours. The obtained extract was cooled to room temperature, filtered through a filter paper, and the filtrate was taken to prepare a mushroom mixed extract consisting of a mushroom mushroom, a mushroom and a mushroom.

Experimental Example 1 Antioxidant Activity

In order to determine the antioxidant activity of the laver or bamboo shoot extract formed in Preparation Example 1 and the mushroom extract, mushroom mushroom, or mushroom extract prepared in Preparation Example 2 was measured using a DPPH radical, which is a method of measuring the antioxidant activity. .

100 μl of laver or bamboo shoots extract and 100 μl of mushroom mixture extract and 0.2 mM DPPH solution as an antioxidant measurement solution were reacted at 37 ° C. for 30 minutes, and then absorbance was measured at 520 nm using an ELISA reader.

Experiments with vitamin C, known as antioxidants, compared the relative activity of extracts. Experimental results As shown in Table 1, it was confirmed that the laver or larvae extract has an antioxidant effect.

Name of sample Vitamin C Lobster Extract Seaweed extract Mushroom Blend Extract SC ₅₀ 100ug / ml 0.7% 0.8% 1.7%

Experimental Example 2 Cytotoxicity Mitigation Effect Test

Human fibroblasts are cultured in medium containing 10% fetal bovine serum for 5 hours by dispensing 5 × 10 ⁴ cells into each well of a 96-well plate. Subsequently, replace with fetal calf serum-free medium, and add the laver or bamboo starch extract and the mushroom mixture extract to a final concentration of 100 ppm in the culture medium, and after 4 hours, 0.02% sodium dodecyl sulfate (SDS) was added. Add and incubate for 24 hours. After incubation, 10 μl of a 5 mg / ml yellow tetrazolium salt (MTT) solution was added per well, and after 4 hours, the yellow tetrazolium salt solution was removed, and dimethyl sulfoxide (DMSO) was added 100 μl per well. Next, the solution was dissolved for 10 minutes and the absorbance of 570 nm was measured using a spectrophotometer (Molecular Devices, USA). The results are shown in Table 2 below.

sample Cell survival rate (%) Negative Control (-SDS) 100.0 Positive control group (+ SDS) 12.5 SDS + Seaweed Extract 69.5 SDS + Locust Extract 68.9 SDS + Mushroom Blend Extract 75.1 SDS + seaweed extract and mushroom mixed extract (1: 1) 88.4 SDS + Buttercup Extract and Mushroom Blend Extract (1: 1) 87.9

As a result of performing the experiment, as shown in Table 2 below, the combination of the red algae extract and the mushroom mixture extract showed an excellent cytotoxic alleviation effect compared to the use of the red algae extract or the mushroom mixture extract.

Experimental Example 3 NO Production

In order to determine the effect of the red algae and mushroom mixture extract of the preparation on the production of NO in RAW 264.7 cells induced oxidative stress with LPS, the following tests were performed. 8 x 10 ⁵ cells were injected into 24 well plates, and the extracts were treated with 100 µg / ml for 2 hours, followed by LPS (1 µg / ml) for 20 hours. 50 μg of 1% selfanilamide and 50 μg of 0.1% naphthylenediamine dihydrochloride were added to 100 μg of the media supernatant, followed by reaction at room temperature for 10 minutes, and the absorbance was measured at 550 nm. At this time, the standard curve was used to prepare NaNO ₂ for each concentration.

Name of sample Untreated extract group Seaweed extract Lobster Extract Mushroom Blend Extract Laver extract and mushroom extract (1: 1) Wood Starfish Extract and Mushroom Extract (1: 1) NO production amount (㎛) 12.1 5.9 5.8 8.2 4.9 4.8

Through the test, it was confirmed that the production of NO was most suppressed when the algae extract and the mushroom mixture extract were treated together.

Experimental Example 4 Lipid Peroxidation

The effect of red algae and mushroom mixture extracts of the preparation on lipid peroxidation in the RAW 264.7 cells induced oxidative stress with LPS was determined by measuring the TBARS content. 8 x 10 ⁵ cells were injected into 24 well plates, and the extracts were treated with 100 µg / ml for 2 hours, followed by LPS (1 µg / ml) for 20 hours. 400 μg of TBARS solution was added to the 200 μg medium supernatant, followed by reaction at 95 ° C. for 30 minutes, centrifugation at 4000 rpm for 10 minutes, and the supernatant was taken to measure absorbance at 532 nm. At this time, the standard curve was prepared by preparing 1,1,3,3-TMP (Tetramethoxypropane) by concentration.

Name of sample Seaweed extract Lobster Extract Mushroom Blend Extract Laver extract and mushroom extract (1: 1) Beetroot Extract and Mushroom Blend Extract (1: 1) TBARS IC ₅₀
(占 퐂 / ml) 248.8 250.9 187.9 118.2 119.7

Through the above test, it was found that the best effect of inhibiting lipid peroxidation when the red seaweed extract or the mantle starch extract and the mushroom mixture extract were treated together.

Preparation Example 5 Examples and Comparative Examples

In the above preparations, a cosmetic composition containing a laver extract or a locust extract (Comparative Examples 1 and 3) and a mushroom mixed extract (Comparative Example 2) and a laver extract or a locust extract and a mushroom mixed extract (Examples 1 and 2) were prepared. To compare with this, a cosmetic composition (Comparative Example 3) lacking an extract was prepared in the following composition ratio.

division Content (% by weight) ingredient Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Seaweed extract 1.0 - 2.0 - - - Lobster Extract - 1.0 - - 2.0 - Mushroom Blend Extract 1.0 1.0 - 2.0 - - Cetearyl Octanoate 5.0 5.0 5.0 5.0 5.0 5.0 Isopropyl Palmitate 5.0 5.0 5.0 5.0 5.0 5.0 Polyacrylamide / C13-14 Isoparaffin / Laureth-7 2.0 2.0 2.0 2.0 2.0 2.0 Polysorbate 60 1.7 1.7 1.7 1.7 1.7 1.7 Solbitan Sesquioleate 1.3 1.3 1.3 1.3 1.3 1.3 Spices, preservatives dose dose dose dose dose dose Purified water Balance Balance Balance Balance Balance Balance Sum 100 100 100 100 100 100

Test Example 5 Anti-inflammatory Test

In order to know the anti-inflammatory effect on the laver extract and mushroom complex extract, five ICR mice were divided into seven groups. Using the right ear of the ICR mouse as a test site, the ear is washed with ethanol before application of the sample, and 20 μl of the sample is continuously applied once a day for 4 days, and 1 mg of arachidonic acid is applied to the right ear 1 hour after the last application. After 1 hour, the degree of edema of the ear was repeatedly measured three times on both ears with a micrometer. The anti-inflammatory effect was determined based on the arachidonic acid treatment group as shown in Equation 2 below, and the results are shown in Table 6 below. The value is shown by the average value of each group.

A: Change of control ear (thickness after sample-thickness of ear before sample treatment)

B: Change of Ear in Sample Treatment Group (Thickness of Ear after Sample Treatment-Ear Thickness Before Sample Treatment)

Glume (㎛) Inhibition rate of inflammation (%) Before sample processing After sample processing Control group 253 551 - Example 1 249 329 73.2 Example 2 254 322 77.2 Comparative Example 1 257 419 45.6 Comparative Example 2 251 347 67.8 Comparative Example 3 256 410 48.3 Comparative Example 4 256 540 4.7

From the results shown in Table 6, it was confirmed that Examples 1 and 2 have the highest anti-inflammatory effect.

Test Example 6 Human Skin Patch Test

In the past, the skin irritation effect was confirmed in human skin patch test of 20 women in their 20s and 30s who had no hypersensitivity reaction to skin irritation and have no skin disease or skin allergy symptoms. Examples 1 and 2 and Comparative Examples 1 to 4 using 5.0% of lactic acid as a skin irritant as an irritant were used. First the test site was wiped with 70% ethanol and dried. 15 μg of the prepared test substance was added dropwise into a Fin chamber (Finn chamber, 100 × 10, EPITEST, Finland), and then airtightly wrapped in the forearm inner part of the test subject. The test site was marked with a marking pen after patching for 24 hours and removing the patch. 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to observe erythema and edema. Skin reactions were determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) and the results are shown in Table 7.

* Evaluation criteria and score of skin reaction

Symbol Score Criteria

  -0 no response

  ± 0.5 faint or light erythema

  + 1 clear but weak erythema, edema and papules

 ++ 2 distinct erythema, papules and vesicles

 +++ 3 Severe erythema and alveolar, scleroderma

Test substance After 1 hour After 24 hours Reactivity (%)
(n = 20) ± + ++ ± + ++ Example 1 + 5% Lactic Acid
Example 2 + 5% Lactic Acid
Comparative Example 1 + 5% Lactic Acid
Comparative Example 2 + 5% Lactic Acid
Comparative Example 3 + 5% Lactic Acid
Comparative Example 4 + 5% Lactic Acid 6--
6--
7 1-
9 1-
9 1-
12 2- One - -
One - -
2 - -
2 - -
2 - -
2 1- 2.92
2.88
4.58
5.42
4.59
8.33

As can be seen in Table 7, Examples 1 and 2 containing the laver extract and mushroom mixture extract of the present invention was found to have a superior skin irritation relief effect induced by lactic acid compared to Comparative Examples 1 to 3.

       On the basis of the results of the previous experimental example, a cosmetic composition containing a red algae extract (steamed laver or manta fern extract) and a mushroom mixture extract is presented. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1 Flexible Lotion

Formulation examples of the flexible lotion containing the red algae extract and the mushroom mixture extract was prepared as shown in Table 8.

Raw material Content (% by weight) Red algae extract and mushroom mixed extract 1.00 1,3-butylene glycol 1.00 Disodium iodide 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Glycereth-26 1.00 Arbutin 2.00 Hydrogenated castor oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

Formulation Example 2 Nutrition Cream

Formulation example of the nutrient cream containing the red algae extract and the mushroom mixture extract was prepared as shown in Table 9.

Raw material Content (% by weight) Red algae extract and mushroom mixture extract 4.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extract 3.2 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlyric / capric triglyceride 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Suitable amount Triethanolamine Suitable amount Preservative Suitable amount Flavoring agent Suitable amount Purified water Balance

Claims (5)

delete delete Antioxidant cosmetic composition containing a mixed extract consisting of laver or young starfish extract and matsutake mushroom, spiritual mushroom and geranium. The cosmetic composition for homeostasis according to claim 3, wherein the content of the mixed extract consisting of the laver or mandarin extract and the mushroom mushroom, mushroom mushroom and germ is 0.1 to 20% by weight relative to the total weight of the cosmetic composition. According to claim 3, wherein the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack Antioxidant cosmetic composition, characterized in that any one selected from the group consisting of soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder and eye shadow.