KR101216151B1 - The composition for cosmetics containing extracts of Lithospermum erythrorhizon and Angelica gigas Nakai - Google Patents

Abstract

The present invention relates to a skin anti-aging and whitening cosmetic composition comprising an extract mixture of licorice and Angelica.
The present invention is basically characterized in that the extract of the licorice and Angelica sinensis contains a stabilized state as a nano liposome as an active ingredient.
According to the present invention, the skin penetration effect is greatly increased because the anti-aging and whitening of the skin as well as the herbal extract mixture is contained by the nanoliposomes.

Description

The composition for cosmetics containing extracts of Lithospermum erythrorhizon and Angelica gigas Nakai}

The present invention relates to a skin anti-aging and whitening cosmetic composition comprising an extract mixture of the licorice and Angelica, more specifically, the skin anti-aging and whitening cosmetic containing the stabilization of the extract mixture consisting of the herb and Angelica extract with nanoliposomes It relates to a composition.

Skin occupies the largest volume in the human body, accounting for about 16% of the human body's volume. The skin is in direct contact with the external environment and serves as an important protective barrier to protect the human body from a variety of environmental variables such as temperature, humidity, antigens and UV light. However, with age, cell proliferation and immune activity are weakened, so that skin damage due to pollutants, strong ultraviolet rays, and stress from the external environment cannot be repaired quickly, resulting in skin wrinkles, loss of elasticity and keratinization.

Skin aging is largely divided into endogenous and exogenous aging [Cosmetics & Toiletories, 111: 31-37 (1996)]. Endogenous aging is an aging phenomenon that occurs naturally as the physiological function of the skin gradually decreases with age. Clinically, the elasticity is decreased, the texture of the skin becomes rough, deep wrinkles are formed, and pigmentation is deposited. Dermatol, 130: 87-95 (1994). Exogenous aging refers to the aging phenomenon caused by exogenous factors such as ultraviolet light, reactive oxygen species and stress.

Living organisms use oxygen to efficiently get the energy they need to survive. However, in the process of converting oxygen into water during this metabolism, free radicals are produced as intermediates. Exemplary free radicals are exposed to superoxide and radiation released by stimulation of macrophages. Hydroxy radicals etc. produced by these etc. are known.

Reactive oxygen species are caused by external factors such as excessive radiation and ultraviolet radiation, ingestion of chemicals and tobacco, and internal factors such as ischemia, reflux, inflammation, stress and aging. The reactive oxygen species generated in this manner are generally highly chemically reactive and easily react with components of adjacent lipids, nucleic acids, and proteins in vivo, leading to various diseases, leading to oxidative disorders. For example, LDL in arteries is oxidized by superoxide to form foam cells, which causes arteriosclerosis. In addition, the production of hydroxy radicals generated by irradiation causes serious obstacles such as carcinogenesis in the living body (Halliwell B. and Gutteridge M. C., Biochem. J. 1984, 219: 1-14).

On the other hand, consumers' awareness of the stability of food additives made of chemical synthetic products as well as antioxidants has recently increased. For example, chemical synthetic antioxidants such as butyl hydroxy anisole (BHA) and butyl hydroxy toluene (BHT) tend to avoid their use. Other antioxidants are often useful (non-aqueous), such as tocopherols derived from vegetable oils, and are often restricted in actual use.

In this situation, cosmetics or skin care agents containing various skin protection ingredients such as retinol, retinoids, vitamin C, flavonoids and tocopherols, and herbal ingredients have been developed to suppress skin aging or slow the progression. In addition, the skin protection agent itself has a big problem in the stability of the cosmetic, such as unstable itself, the sedimentation of the contents in the final formulation and the change in color, the potency is lowered and the efficacy is slight.

In the present invention, to solve this problem, but using the licorice and Angelica extract, it utilizes a liposome form that can be delivered stably without destroying the physiologically active component and can contain both the fat-soluble or water-soluble. Prior to the present invention, porcelain was used as soap (Korean Patent Application No. 2008-0023164), and Angelica was used for preventing skin aging (Korean Patent Publication No. 2005-0073918). However, cosmetics stabilized with nanoliposomes containing both licorice and Angelica as in the present invention have not been studied to the extent that industrialization is possible.

An object of the present invention is to provide a skin aging prevention and whitening cosmetic composition containing the nano liposomes stabilized by the mixture of the licorice and Angelica.

The present inventors have diligently researched to develop a skin anti-aging and whitening cosmetic composition mixed with the extract of Licorice and Angelica extract, and it was found that the extract of Licorice and Angelica extract promotes the proliferation of skin cells, antioxidant activity, collagen synthesis, skin immunity enhancement and skin moisturizing. There is an improvement effect, the cosmetics containing the nanoliposome stabilized in the herbal extract mixture as an active ingredient greatly increases the skin penetration effect, is maintained stable in the cosmetic composition, wrinkles generated, reduced elasticity and moisturizing ability when applying skin The present invention was completed by confirming that there is an excellent anti-aging effect and a whitening effect by eliminating complex skin aging phenomenon such as loss.

The anti-aging and whitening cosmetic composition of the present invention as described above, characterized in that the extract of the herbaceous and Angelica gigas contains a stabilized state as a nanoliposome as an active ingredient.

In this case, the nanoliposomes are 1 to 30 nm in diameter, characterized in that contained in 0.1 to 20% by weight relative to the total weight of the cosmetic composition.

More preferably, the nano liposomes are characterized in that the diameter is 100 ~ 200nm and contained 4 to 12% by weight based on the total weight of the cosmetic composition.

In the present invention, the relative mixing ratio of Licorice and Angelica is 2: 3 to 3: 2, and the mixture of Licorice and Angelica extract is characterized in that it contains 0.1 to 20% by weight relative to the total weight of nanoliposomes.

In this case, the phospholipid used for the preparation of nanoliposomes is lecithin, the amount of which is used is 0.5 to 20% by weight based on the total weight of nanoliposomes, and the amount of water used for the preparation of nanoliposomes is 5.0 to 40% by weight based on the total weight of nanoliposomes. It is characterized by.

In addition, the nano-liposomes are prepared by applying a mixture of licorice and Angelica extract to the ultrasonic emitter, the production conditions are characterized in that the operation for 30 minutes through the release for 7 seconds, stop for 5 seconds under a pulse of 32 ~ 42Mhz.

Skin anti-aging and whitening cosmetic composition containing nanoliposomes stabilized herbal extract extract consisting of the herbaceous and Angelica extract according to the present invention as an active ingredient, skin whitening effect by promoting the proliferation of skin cells, antioxidant effect, inhibition of melanin synthesis Prevents skin aging by improving skin elasticity, improves moisturizing power and enhances skin immunity. Since herbal extract mixture is contained by nanoliposomes, the skin penetration effect is greatly increased and the effect of active ingredient is maximized. Greatly improved.

The present invention provides a cosmetic composition for preventing skin aging, comprising nanoliposomes stabilized with the herbaceous and Angelica extract mixture (hereinafter referred to as "herbal mixture") as an active ingredient. The inventors of the present invention conducted research on herb and donkey which have been used as a herbal medicine in the past, and as a result, the herbal mixture is excellent in promoting skin cell proliferation, antioxidant activity, collagen synthesis promotion, skin immunity enhancement and skin moisturizing effect. It was confirmed that the present invention was completed by stabilizing with nanoliposomes to maximize the effect.

The "herbal mixture" provided in the present invention is obtained by mixing a licorice and Angelica extract.

Lilium (Lithospermum erythrorhizon) is a perennial herb of the calliphytic denture clinic, also known as lichen (芝 草). It is 30 ~ 70㎝ high, and its roots are deep in the ground, and it is thick and purple. Stems and leaves have rough hairs. Leaves are alternate, without sack, basaloid, 3 ~ 7㎝ long, 1 ~ 2㎝ butterfly, clear with 2 ~ 3 side veins. Flowers bloom white in May-June and run in inflorescences. Bracts are leaf-shaped, sepals are 5 broad linear, longer than corolla. Corollas are shallowly divided into 5 ends and spread almost horizontally. There are 5 stamens, 1 pistil, and the ovary has a cruciform groove. Fruits are greyish white, glossy. Dried roots of chichi are called roots (紫 根), which is used in oriental medicine to detox, fever, swelling, burns, frostbite, eczema and blisters. In civilian use, it is used as a weak medicine. In the past, it was used as a purple dye or food coloring. It grows in dry grass or mountain areas. It is distributed throughout Korea, Japan, and Dongbei [Northeast] China.

Angelica gigas Nakai is the root of the perennial aromatic herb, the genus Angelica. Looking at the plant shape of the donkey 60-90㎝ high. Flowers are white and bloom at the end of main stems and branches in August-September. Fruits are long, long oval, with narrow wings at the edges. Angelica is a representative herbal medicine of blood remedies among herbal medicines to send blood and improve the clearing and blood flow. Therefore, it is greatly used for pain resulting from anemia or blood circulation disorder caused by blood. In addition, it promotes the peristalsis of the intestines, and smoothly discharge the gas in the body helps to absorb sufficient nutrition. The components of Angelica mucin include essential oils and pyranucmarins of decursinol, decursin, nadakenetine, umbeliferon, beta-sitosterol and other water-soluble nonvolatile crystals.

The herbal ingredient of the "herbal mixture" provided in the present invention may be obtained using various extraction methods known in the art, and preferably, 70% ethanol or distilled water may be obtained as an extraction solvent. In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. The herbal extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

In the present invention, the nanoliposome content stabilized the herbal extract mixture is 0.1 to 20% by weight, preferably 4 to 12% by weight based on the total weight of the cosmetic composition.

As used herein, the term "nanoliposomes" refers to liposomes having an average particle diameter of 1 to 300 nm as having a conventional liposome form. According to a preferred embodiment of the invention, the average particle diameter of the nanoliposomes is 100 ~ 200nm. When the average particle diameter of the nanoliposomes exceeds 200nm, the improvement of skin penetration and the improvement of formulation stability are weak among the technical effects to be achieved in the present invention.

Phospholipids used in the preparation of the nanoliposomes of the present invention are used as amphoteric lipids, and include natural phospholipids and synthetic phospholipids, preferably lecithin. More preferably, the lecithin is naturally occurring unsaturated lecithin or saturated lecithin extracted from soybean or egg yolk. Typically, lecithin derived from nature has 23-95% phosphatidylcholine and 20% or less phosphatidylethanolamine.

In the preparation of the nanoliposomes of the present invention, the amount of lecithin is 0.5 to 20% by weight, preferably 2 to 8% by weight based on the total weight of the nanoliposomes.

The water used in the preparation of the nanoliposomes of the present invention is generally deionized distilled water, the amount of which is 5.0 to 40% by weight relative to the total weight of the nanoliposomes.

The preparation of nanoliposomes may be accomplished through various methods known in the art, but most preferably, a mixture comprising the above components is applied to an ultrasonic emitter. The preparation of nanoliposomes by ultrasonic emitter can be carried out under various conditions (eg, pulse, time, etc.) according to the desired particle size. Run for minutes to prepare nanoliposomes.

The nanoliposome system thus prepared has the advantage of maximizing the skin penetration effect by dissolving various types of poorly soluble substances and stabilizing unstable substances.

The components included in the cosmetic composition of the present invention include components conventionally used in cosmetic compositions, in addition to nanoliposomes containing the herbal extract mixture as an active ingredient, and include, for example, conventional agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. Adjuvants, and carriers.

The cosmetic composition of the present invention prevents skin aging through skin whitening and skin immunity enhancement by promoting the proliferation of skin cells, antioxidant efficacy, and inhibition of melanin synthesis, which is specified in the following examples. Moreover, the herbal extract mixture in the cosmetic composition of the present invention is contained by the nanoliposomes, so the skin penetration effect is greatly increased and the stability of the formulation is also improved.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Self-explanatory

Preparation Example 1 Preparation of "Medicinal Herb Mixture"

Firstly, after completely drying the porridge and the Angelica gigas, 50g of the grass, and 50g of the Angelica gilt each were mixed to dry weight, and then 70% ethanol as an extraction solvent was added 10 to 20 times by volume. It was heated at 60 ° C. for about 24 hours. After filtration, cooled to room temperature, and then aged at low temperature for 4 days at 4 ℃ was used.

Experimental Example 1 skin cell proliferation effect

In order to examine the effect of the "herbal mixture" on skin cell proliferation, human normal fibroblasts (Korea Cell Line Bank) were inoculated to 1 x 10 ⁴ cells in each well of a 96-well microplate, Incubated at 37 ° C. for 24 hours in RPMI1640 medium (Sigma). Subsequently, the herbal extract mixture was replaced with the serum-free RPMI1640 medium without the herbal extract mixture and the experimental group replaced with the serum-free RPMI1640 medium containing the final concentrations of 0.2%, 0.4%, 0.6%, 08% and 1%. One control was further incubated for 24 hours. Then, 10 μl each of MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added thereto, followed by incubation for 4 hours, The medium was removed, shaken for 20 minutes by addition of 100 μl of DMSO solution per well, and the absorbance of the supernatant was measured at 517 nm using a microplate reader (Molecular Devices, USA). The measured values were substituted into the following Equation 1 to calculate the cell proliferation effect, and the results are shown in Table 1 below.

As can be seen in Table 1, compared to the control group not treated with the herbal extract mixture, the experimental group treated with the herbal extract mixture showed a very high fibroblast proliferation effect.

Experimental Example 2: Antioxidant Effect

Free Radical Scavenging Activity Test was conducted to investigate the antioxidant effect of the "herbal mixture" obtained according to the preparation example of the present invention.

The free radical scavenging activity was modified from the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299-303 (1993)), which is a stable free radical DPPH (1,1-diphenyl-2-picryhydrazyl , Sigma) reagent was used. 150 ㎕ of DPPH solution (ethanol in the case of Blanc) was prepared in various concentrations of the herbal extract mixture, and 150 을 of each of them was added and mixed, and left at room temperature for 30 minutes, and then an experimental group was measured to measure absorbance at 517 nm. As a control for this was set to the case using purified water. After measuring the absorbance for the experimental group and the control group, the free radical scavenging activity was obtained using Equation 2 below and the results are shown in Table 2 below.

As can be seen from the results shown in Table 2, the herbal extract mixture of the present invention was confirmed that the free radical scavenging ability is very excellent.

Experimental Example 3 Exploration of Whitening Effect by Tyrosinase Inhibition

Melanin is produced by the biosynthesis process of an enzyme called tyrosinase in cells in the skin and increases the skin's resistance to ultraviolet rays, dryness, and extreme temperatures, but excessive melanin production causes pigmentation such as blemishes, freckles, and scabs in the human body. Promote damage. Therefore, it is a very important part in measuring the skin whitening effect in the cosmetic industry. The inventors measured the effect of tyrosinase inhibition using the dopachrome method.

After incubation with 150 μl of mushroom tyrosinase-150 unit, 225 μl of 2.5 mM L-tyrosine, 225 μl of 0.4M hepes buffer (pH 6.8), and 300 μl of ethanol solution or sample solution (1 mg / ml). After 15 minutes of incubation, the absorbance was measured at 475 nm to examine the degree of inhibition.

The degree of inhibition of tyrosinase was calculated as follows.

In the above formula, A and B are the absorbances before and after incubation of the solution to which the sample is added, respectively, and C and D are the absorbances before and after incubation of the solution (reference solution) without the sample. These tyrosinase inhibitory effects indicate complete inhibition at 100, and no inhibition at all.

Table 3 shows the comparison of the inhibition rate when the sample is added at a concentration of 1.0 mg / ml.

 It was confirmed that the inhibition rate gradually increased in all samples in a concentration-dependent manner, and the positive control group ascorbic acid showed the highest inhibition rate of 70.8% at the concentration of 1.0 mg / mL. The extract of Angelica gigas and S. ginseng showed high inhibition rate of 69.4%, which is close to ascorbic acid.

As a result, it was found that the inhibitory effect of tyrosinase enzyme activity was increased due to the synergistic effect of S. aureus and Angelica. Therefore, it can be confirmed that the extract of Angelica sinensis and the herbaceous extract have an effect on skin whitening activity and can enhance the activity.

Preparation Example 2 Preparation of Nanoliposomes Containing Herbal Extract Mixture

The nanoliposome was added to phase B in the composition of Table 4 below and operated for 30 minutes through the ultrasonic emitter 32 Mhz, stopping for 7 seconds for 5 seconds to obtain a nanoliposome that stabilized the herbal extract mixture of 100-200 nm particle size. .

Experimental Example 4 Observation of the Absorption of Nanoliposomes

After adding nanoparticles containing the licorice and donkey mixture prepared by Preparation Example 2 to fibroblasts (ccd986sk) cultured using RPMI1640 medium in a 37% incubator with 5% carbon dioxide, the luminous flux from the light source was focused. Scanning Confocal Microscope, which is irradiated onto the sample surface and detects the change in the sample depth as the change in the light intensity, is implemented as an image.

As a result, it was observed that the nanoparticles were absorbed into the cells as shown in the photograph. Through this, it can be confirmed that cell absorption of the nanoparticles is easy, and it can be seen that the effect is good even in vivo.

Photo 1. Observation of Intracellular Uptake of Nanoparticles by Scanning Confocal Microscope

The inventors also added nanoparticles containing the licorice and donkey mixture prepared by Preparation Example 2 to fibroblasts (ccd986sk) cultured using RPMI1640 medium in a 5% carbon dioxide, 37 ° C. incubator, followed by uranyl acetate and Double staining with lead citrate was observed at 100kv with JEOL 200CX transmission electron microscope.

As a result, it was observed that the nanoparticles were absorbed into the cells as shown in the photograph. Through this, it was confirmed that the cell uptake of nanoparticles was easy, and therefore, the effect would be good in vivo.

Photo 2. Observation of intracellular uptake of nanoparticles using transmission electron microscope

Formulation Example  1. Massage cream Formulation Example  example

As shown in the following table, it is possible to prepare a massage cream according to a conventional method.

Claims (6)

delete delete delete delete delete The extracts of S. lichen and Angelica are contained as an active ingredient in a stabilized state as nanoliposomes, wherein the nanoliposomes are 100 to 200 nm in diameter and 4 to 12 wt% based on the total weight of the cosmetic composition;
At this time, the relative mixing ratio of the licorice and Angelica is 2: 3 to 3: 2 and the mixture of the licorice and Angelica extract is contained 0.1 to 20% by weight relative to the total weight of the nano liposomes;
Phospholipids used in the preparation of the nanoliposomes are lecithin and the amount of the phospholipids is 0.5 to 20% by weight based on the total weight of the nanoliposomes, and the amount of water used for the preparation of the nanoliposomes is 5.0 to 40% by weight based on the total weight of the nanoliposomes. ;
In addition, the nano-liposomes are prepared by applying a mixture of licorice and Angelica extract to the ultrasonic emitter, the manufacturing conditions are characterized in that the operation for 30 minutes through the release for 7 seconds, stop for 5 seconds under a pulse of 32 ~ 42Mhz,
Cosmetic composition for preventing skin aging and whitening.