KR102261115B1 - Cosmetic composition with the extract of Salicornia herbacea L., the extract of Liriope platyphylla and the extract of Eleutherococcus senticosus for improving skin wrinkle - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin wrinkle reduction comprising a Salicornia europaea extract, a Liriope muscari extract, and an Eleutherococcus senticosus extract, and exhibits a substantial skin elasticity enhancement and wrinkle reduction effect. In addition, the present invention also exerts an anti-aging effect on the skin due to its antioxidant effect. In addition, the present invention does not cause safety problems such as irritation and redness when applied to the skin, and has the advantage of excellent formulation stability.

Description

A cosmetic composition for improving skin wrinkles comprising a green tea extract, a maekmundong extract, and an extract of S. oleracea {Cosmetic composition with the extract of Salicornia herbacea L., the extract of Liriope platyphylla and the extract of Eleutherococcus senticosus for improving skin wrinkle}

The present invention relates to a cosmetic composition for improving skin wrinkles, and more particularly, to a cosmetic composition for improving skin wrinkles, comprising a green tea extract, a maekmundong extract, and an extract of S.

Collagen is a major matrix protein produced by fibroblasts in the skin and is present in the extracellular matrix. Its important functions include mechanical firmness of the skin, resistance of connective tissue and tissue adhesion, support of cell adhesion, cell division and differentiation. Induction (at the time of growth of an organism or wound healing) is known. Such collagen is reduced by age and photoaging caused by UV irradiation, and collagen reduction is promoted by collagenase enzyme activity that decomposes collagen. This is known to be closely related to the formation of wrinkles on the skin.

Elastin fibers form cross-links with collagen and are an important skin component for wrinkle creation, which is involved in skin elasticity. Deficiency and aggregation of elastin fibers, and a marked increase in the activity of the elastin-degrading enzyme, elastase, have been found to be one of the factors for generating skin wrinkles. Elastase is the only enzyme capable of decomposing elastin, and inhibition thereof is known to fundamentally reduce skin wrinkle improvement.

Collagen and elastin fibers are matrix proteins that play an important role in retaining moisture in the dermis layer. These matrix proteins adsorb moisture, and increase moisture retention within their structures, allowing the skin to maintain proper moisture content. This is known to be involved in maintaining the elasticity of the skin.

Currently, retinoid, adenosine, animal placenta-derived protein, chlorella extract, etc. are known as skin elasticity enhancement and wrinkle improvement cosmetics. Retinol, the most well-known, is a substance that promotes collagen synthesis and inhibits the elastase enzyme, but it is unstable, and the amount of retinol used is limited due to safety issues such as irritation and redness when applied to the skin. It is known that it is difficult to expect the effect of improving elasticity and improving wrinkles.

Korean Patent Publication No. 1020110048378 (published on May 11, 2011), relates to a cosmetic composition for wrinkle improvement or elasticity enhancement containing a green tea callus extract and a channel drag extract, and more specifically, a green tea callus extract and a channel drag extract. A technique for effectively improving skin wrinkles and elasticity by synergistic action of the two active ingredients as well as improving moisturizing power is described. Republic of Korea Patent Registration No. 1013634130000 (registration date 2014.02.10) relates to a cosmetic composition for preventing skin aging comprising a mixed extract of green tea and nogeun and a method for manufacturing the same, and the mixed extract of green tea and nogeun is a natural substance and is cytotoxic There is no collagenase activity inhibitory effect and collagen production promotion effect is excellent, and when administered to the skin, skin wrinkles and skin texture can be improved, so a technique that can be usefully used as a cosmetic for skin aging is described.

In the present invention, as a composition that exhibits substantial skin elasticity enhancement and wrinkle improvement effects, it is intended to develop and provide a cosmetic composition that does not cause safety problems such as irritation and redness and formulation stability problems when applied to the skin.

The present invention provides a cosmetic composition for improving skin wrinkles, characterized in that it comprises a green tea extract, a maekmundong extract, and an extract of S.

In the cosmetic composition for skin wrinkle improvement of the present invention, the green tea extract, maekmundong extract, and spiny cyst extract are preferably 5.2 to 8.5 parts by weight of green tea extract, 1.2 to 4.5 parts by weight of maekmundong extract, 2.0 to 3.5 parts by weight of oleifera extract. It is better to mix them in proportions.

In the cosmetic for improving skin wrinkles of the present invention, the cosmetic composition is preferably also for skin aging prevention.

In the cosmetic for improving skin wrinkles of the present invention, the composition is preferably selected from the group consisting of lotions, emulsions, creams, foams, gels, nutrient lotions, softening lotions, packs, softening water, emulsions, essences, ampoules, and suspensions. It may have any one formulation.

The composition of the present invention exhibits a substantial skin elasticity enhancement and wrinkle improvement effect. In addition, it also exerts an anti-aging effect on the skin due to its antioxidant effect. In addition, the present invention does not cause safety problems such as irritation and redness when applied to the skin, and has the advantage of excellent formulation stability.

1 is a cytotoxicity test result of a complex of the present invention green tea extract, maekmundong extract, and oleracea extract.
Figure 2 is a Procollagen ELISA test result of the complex of the present invention green tea extract, maekmundong extract, and a spiny persimmon extract.
3 is a DPPH test result of a complex of the present invention green tea extract, maekmundong extract, and spleenaceae extract.
4 is a photograph of a lotion formulation and a cream formulation of the present invention made by applying a complex of the present invention green tea extract, maekmundong extract, and spiny hornwort extract.
5 is a photograph of the sunscreen and BB cream formulations of the present invention made by applying a complex of the present invention green tea extract, maekmundong extract, and spiny hornwort extract.
6 is a photograph of the toner and mask impregnating liquid formulations of the present invention made by applying a complex of the present invention green tea extract, maekmundong extract, and spiny persimmon extract.
7 is a formulation stability test result of a complex in which the green tea extract of the present invention, maekmundong extract, and S.

The present invention provides a cosmetic composition for improving skin wrinkles, characterized in that it comprises a green tea extract, a maekmundong extract, and an extract of S.

In the present invention, it was confirmed that the expression of procollagen increased in a concentration-dependent manner in the complex to which all of green tea, maekmundong, and spiny hornwort were added, and it was confirmed that the antioxidant effect was increased in a concentration-dependent manner. Based on this, it was confirmed that the cosmetic composition of the present invention exerts a significant effect in improving skin wrinkles or preventing skin aging. In particular, it was confirmed that all of green tea, maekmundong, and scallops were added, and compared to the other cases, formulation stability was not abnormal in all of room temperature stability, high temperature stability, low temperature stability, and light stability.

Green tea ( Salicornia herbacea L. ) is an annual plant belonging to the family Plumaceae. It is also called tung-tung joint because its leaves and stems are bulging and the nodes are protruding, and it is known to be effective in hypertension and diabetes. It has been reported to inhibit the proliferation of P. acnes, the causative agent of acne, and has been reported to have antioxidant and tyrosinase activity inhibitory properties. In relation to skin beauty, green tea is known to have effects such as acne bacteria growth inhibition, DPPH radical scavenging activity and antioxidant activity, tyrosinase inhibitory activity, skin astringent effect, and melanoma cell inhibition. It is effective for diarrhoea, constipation, and obesity, and is known to be effective in promoting gastric function, nephritis, hepatitis and hypertension, hypotension, anemia, hypoglycemia, and thyroid function improvement. In the present invention, it is preferable to use the leaves and stems of green tea.

Maekmundong ( Liriope platyphylla ) is a perennial plant of the order Bizarumaceae. It is distributed in Korea, Taiwan, and Japan, grows in the shade of trees in mountainous areas, and its roots are mainly used as medicinal in oriental medicine. In addition, anti-inflammatory and anticancer effects have been reported. In the present invention, it is preferable to use the root of maekmundong.

Eleutherococcus senticosus is a shrub belonging to the genus Eleutherococcus senticosus, which is resistant to cold and grows well in shaded places with good drainage. It is distributed throughout the Korean Peninsula, Far East Russia, Northeast China, and Hokkaido, Japan. Flowers in June-July. It is characterized by many needle-like long and thin thorns on the stem, and is reported to be rich in useful medicinal substances. In the present invention, it is preferable to use the root of St.

The present inventors conducted research to develop a material with better skin elasticity, wrinkle improvement, moisturizing, antioxidant and skin improvement effects by simultaneously adding maekmundong extract and spiny cypress extract to naturally occurring green tea extract. It was found that a composition containing the complex extract as an active ingredient promotes collagen synthesis, inhibits elastase activity, exhibits an antioxidant effect, and a moisturizing effect, and a cosmetic composition using the composition has been completed.

As used herein, the term "extract" refers to an extract obtained by extraction of the raw material, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a prepared or purified product of the extract, or a mixture thereof, etc. , including the extract itself and extracts of all formulations that can be formed using the extract. The extract of the present invention may be extracted from a natural, hybrid or mutated plant of each of the corresponding plants, and may also be extracted from a plant tissue culture. In the extraction of the raw material of the present invention, the method of extracting the extract is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a general extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, and these may be performed alone or in combination of two or more methods.

The type of the extraction solvent used to extract the raw material in the present invention is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; C1-C4 lower alcohols, such as methanol, ethanol, propyl alcohol, and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Alternatively, a mixture thereof may be used, and preferably, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in a mixture of two or more.

In the present invention, the extract extracted with hot water or chilled is filtered to remove floating solid particles, for example, using nylon or the like to filter the particles or using freeze filtration, etc., and then used as it is, freeze-dried or hot-air dried. , spray drying, etc. can be used to dry it.

In the cosmetic for skin wrinkle improvement of the present invention, the green tea extract, maekmundong extract, and spiny persimmon extract preferably contain 5.2 to 8.5 parts by weight of green tea extract, 1.2 to 4.5 parts by weight of maekmundong extract, and 2.0 to 3.5 parts by weight of spearmint extract. It is better to mix with In such a compounding ratio, the skin wrinkle improvement and skin aging prevention efficacy of the present invention can be maximized.

On the other hand, according to the experiment of the present invention, the cosmetic for improving skin wrinkles of the present invention has the effect of preventing skin aging in addition to improving skin wrinkles.

The cosmetic composition for improving skin wrinkles of the present invention is not limited to a particular formulation, but preferably a lotion, emulsion, cream, foam, gel, nutrient lotion, softening lotion, pack, softening water, emulsion, essence, ampoule, suspension It may have any one formulation selected from the group consisting of.

Hereinafter, the contents of the present invention will be described in more detail through the following Examples, Experimental Examples and Formulation Examples. However, the scope of the present invention is not limited to the following Examples, Experimental Examples and Formulation Examples, and includes modifications of technical ideas equivalent thereto.

[Example 1: Preparation of the composite of the present invention]

A complex was prepared by mixing 7 parts by weight of green tea (leaf and stem) extract, 3 parts by weight of maekmundong (root) extract, and 3 parts by weight of S. oleracea (root) extract. All of these extracts are medium temperature hot water extracts at about 60°C.

[Experimental Example 1: Cytotoxicity test of the compound of the present invention]

CCD986sk cells are selected and seeded in a 96-well plate with 3*10^3. After 24 hours, serum free media was treated. The samples were then processed. After 48 hours of sample treatment, cytotoxicity was performed. After 2 hours of treatment with WST solution, absorbance was measured at 450 nm with a multi-reader. Absorbance was expressed as a percentage of cell viability.

The experimental results were the same as in FIG. 1 , but green tea, maekmundong, spiny persimmon extract, and three types of complex had no cytotoxicity in the 5% treatment group or less, but rather had a cell proliferation effect. "conentration (%)" described on the horizontal axis of FIG. 1 means the treatment concentration of the sample. To make a sample, first, 100 ul of the compound of Example 1 was taken and mixed with 900 ul of serum free media to make a 10% treatment group. This was diluted by 1/2 using serum free media to make 5%, 2.5%, 1.25%, 0.625%, 0.3125% treatment groups and tested.

[Experimental Example 2: Procollagen ELISA test of the compound of the present invention]

Seed CCD986sk cells in a 24-well plate at 1.5*10^4. After 24 hours, serum free media was treated. Thereafter, aging was performed. After 48 hours of sample treatment, the culture medium was recovered and Procollagen ELISA was performed according to the kit manual.

The experimental results were as shown in FIG. 2 . "conentration (%)" described on the horizontal axis of FIG. 2 means the treatment concentration of the sample. To make a sample, first, 100 ul of the compound of Example 1 was taken and mixed with 900 ul of serum free media to make a 10% treatment group. This was diluted by 1/2 using serum free media to make 5%, 2.5%, 1.25%, 0.625%, 0.3125% treatment groups and tested.

The green tea extract showed the highest procollagen expression at 0.625%, and the expression level decreased at subsequent concentrations, but the expression level was higher than that of the control by about 20% or more. The procollagen expression was increased in a concentration-dependent manner in the extracts of Maekmundong and St. spinach, but it was not significant compared to the green tea extract. The three complexes showed a concentration-dependent increase in procollagen expression, and the 2.5% treatment group had a significant 28% higher expression than the control group. (TGF-b is used as a positive control)

[Experimental Example 3: DPPH test of the compound of the present invention]

250 μl of 0.1 mM DPPH, 200 μl of EtOH, and 50 μl of the sample were put into each, and reacted at 4° C. for 30 minutes. After 30 minutes of reaction, 100 μl of each was transferred to a 96×well plate and absorbance was measured at 520 nm. Ascorbic acid and Gallic acid were used as positive controls.

The experimental results were as shown in FIG. 3 . "conentration (%)" described on the horizontal axis of FIG. 3 means the treatment concentration of the sample. To make a sample, first, purified water was added to the complex of Example 1 and diluted by 1/2. 0.1mM DPPH 250 μl, each diluted sample in 200 μl EtOH was put in 50 μl to prepare samples with final concentrations of 5%, 2.5%, 1.25%, 0.625%, 0.3125%

The antioxidant effect was increased in a concentration-dependent manner in the extracts of green tea, maekmundong, and spiny cysts (Gammondong IC ₅₀ = 2.15%, maekmundong IC ₅₀ = 4.49%, spiny cyst IC ₅₀ = 2.52%, three kinds of complex IC ₅₀ = 2.02%). . Ascorbic acid was used as a positive control (IC ₅₀ = 7.25ug/ml).

[Formulation Example 1: Preparation of a cosmetic composition containing the complex of the present invention [Lotion]]

In this formulation example, a lotion was prepared using the complex of Example 1. The purified water of this preparation example was added so that the total amount of the cosmetic composition was 100 ml. Add 3.0 g of glycerin and 0.05 g of trehalose to purified water heated to 80° C. and stir to dissolve it completely, then stir at a constant speed to dissolve 1.0 g of cetyl alcohol, glyceryl stearate A mixture of 0.78 g of Glyceryl Stearate and 1.0 g of Cetyl Ethylhexanoate was slowly added to emulsify. Then, C13-14 isoparaffin (C13-14 Isoparaffin) 0.07 g, polyacrylamide (Polyacrylamide) 0.14 g, cyclohexasiloxane (Cyclohexasiloxane) 0.09 g slowly added, stirred to dissolve completely, the temperature was 40-50 At a constant rate at ℃, 1.0 ~ 5.0 g of the compound of Example 1 and 2.0 g of 1,2-hexanediol (1,2-Hexanediol) were sequentially added and administered while stirring. Then, after cooling to room temperature and defoaming, a lotion was prepared (FIG. 4).

[Formulation Example 2: Preparation of a cosmetic composition containing the complex of the present invention [cream]]

In this formulation example, a cream was prepared using the complex of Example 1. The purified water of this preparation example was added so that the total amount of the cosmetic composition was 100 ml. After adding 4.0 g of glycerin and 1.0 g of betaine to ㎖ of purified water heated to 80 ℃ (to100) and stirring to dissolve completely, 0.52 g of cetyl palmitate dissolved while stirring at a constant speed, Sorbitan Palmitate 0.39 g, Sorbitan Olivate 0.39 g, Behenyl Alcohol 0.36 g, Arachidyl Alcohol 0.6 g, Arachidyl Glucoside 0.24 g , a mixture of 2.0 g of cetyl alcohol, 2.0 g of triethylhexanoin, and 5.0 g of cetyl ethylhexanoate was slowly added to emulsify. After that, 0.38 g of cyclopentasiloxane and 0.52 g of sodium polyacrylate are slowly added, stirred to dissolve completely, and the compound of Example 1 is prepared at a constant rate at a temperature of 40 to 50 ° C. 1.0 ~ 5.0 g, 1,2-hexanediol (1,2-Hexanediol) 2.0 g was added in order and administered while stirring. Then, after cooling to room temperature and defoaming, a cream was prepared (FIG. 4).

[Formulation Example 3: Preparation of a cosmetic composition containing the complex of the present invention [sunscreen]]

In this formulation example, a sunscreen was prepared using the complex of Example 1. The purified water of this preparation example was added so that the total amount of the cosmetic composition was 100 ml. After adding g to ㎖ of purified water (to100) heated to 80 ℃ and stirring to dissolve completely, 0.52 g of cetyl palmitate dissolved while stirring at a constant speed, sorbitan palmitate 0.39 g, sorbitanol Sorbitan Olivate 0.39 g, Behenyl Alcohol 0.36 g, Dispersed Titanium Dioxide, Ethylhexyl Methoxycinnamate 7.50 g, Triethylhexanoin 2.0 g, A mixture of 5.0 g of cetyl ethyl hexanoate was slowly added to emulsify. After that, 0.38 g of cyclopentasiloxane and 0.52 g of sodium polyacrylate are slowly added, stirred to dissolve completely, and the compound of Example 1 is prepared at a constant rate at a temperature of 40 to 50 ° C. 1.0 ~ 5.0 g, 1,2-hexanediol (1,2-Hexanediol) 2.0 g was added in order and administered while stirring. Then, after cooling to room temperature and defoaming, a sunscreen was prepared (FIG. 5).

[Formulation Example 4: Preparation of a cosmetic composition containing the present invention complex [BB Cream]]

In this formulation example, a BB cream was prepared using the complex of Example 1. The purified water of this preparation example was added so that the total amount of the cosmetic composition was 100 ml. Add g to ㎖ of purified water (to100) heated to 80° C., stir to dissolve completely, and then stir at a constant speed to dissolve 0.52 g of Cetyl Palmitate, 0.39 g of Sorbitan Palmitate, and sorbitanol. Sorbitan Olivate 0.39 g, Behenyl Alcohol 0.36 g, Dispersed Titanium Dioxide, Yellow Iron Oxide (CI 77492), Red Iron Oxide (CI 77491), Black Iron Oxide (CI 77499), Ethyl Hex A mixture of 7.50 g of Ethylhexyl Methoxycinnamate, 2.0 g of Triethylhexanoin, and 5.0 g of Cetyl Ethylhexanoate was slowly added to emulsify. After that, 0.38 g of cyclopentasiloxane and 0.52 g of sodium polyacrylate are slowly added, stirred to dissolve completely, and the compound of Example 1 is prepared at a constant rate at a temperature of 40 to 50 ° C. 1.0 ~ 5.0 g, 1,2-hexanediol (1,2-Hexanediol) 2.0 g was added in order and administered while stirring. Then, after cooling to room temperature and defoaming, a BB cream was prepared (FIG. 5).

[Formulation Example 5: Preparation of a cosmetic composition containing the complex of the present invention [toner]]

In this formulation example, a lotion was prepared using the complex of Example 1. The purified water of this preparation example was added so that the total amount of the cosmetic composition was 100 ml. After adding 3.0 g of glycerin and 0.05 g of trehalose to purified water heated to 80° C. and stirring to dissolve it completely, PEG-60 Hydrogenated Castor Oil (PEG-) dissolved while stirring at a constant speed 60 Hydrogenated Castor Oil) 1.0 g and a mixture of fragrance were slowly added to solubilize. Afterwards, 1.0 to 5.0 g of the compound of Example 1 and 2.0 g of 1,2-hexanediol (1,2-Hexanediol) were sequentially added to the compound of Example 1 at a constant rate at a temperature of 40 to 50° C., and administered while stirring. Then, after cooling to room temperature and defoaming, a lotion was prepared (FIG. 6).

[Formulation Example 6: Preparation of a cosmetic composition containing the complex of the present invention [mask solution]]

In this formulation example, a mask impregnation solution was prepared using the complex of Example 1. The purified water of this preparation example was added so that the total amount of the cosmetic composition was 100 ml. Glycerin 3.0 g, trehalose 0.05 g, and carbomer 0.15 g are added to purified water heated to 80° C. and completely dissolved by stirring, and then dissolved PEG-60 while stirring at a constant speed. Hydrogenated castor oil (PEG-60 Hydrogenated Castor Oil) 1.0 g, a mixture of fragrance (Fragrance) was slowly added to solubilize. After that, 1.0 to 5.0 g of the compound of Example 1 and 2.0 g of 1,2-hexanediol (1,2-Hexanediol) were sequentially added to the compound of Example 1 at a constant rate when the temperature was 40 to 50° C., and administered while stirring. Then, after cooling to room temperature and defoaming, a mask impregnating solution was prepared (FIG. 6).

[Experimental Example 4: Formulation Stability Test]

In this experimental example, the cream formulation of Formulation Example 2 (in this experiment, based on Formulation Example 2, 10 wt% of each each), and the toner formulation of Formulation Example 5 (in this experiment, based on Formulation Example 5, but with green tea, 'Gamcho, Maekmundong', 'Green tea, Spiny mildew', 'Hamcho, Maekmundong, Spiny oleifera', respectively. Formulation stability was tested for 10% by weight).

The result was as shown in FIG. 7 . As shown in FIG. 7 , when using green tea alone, or adding maekmundong to green tea, or adding scallops to green tea, it was confirmed that stability, especially at high temperature, was lowered. However, in the case of the composite material containing all of green tea, maekmundong, and chrysanthemum, it was confirmed that all were not abnormal in room temperature stability, high temperature stability, low temperature stability, and light stability.

Claims (4)

Contains green tea extract, maekmundong extract, and spiny persimmon extract,
The green tea extract, maekmundong extract, and oleracea extract use water as an extraction solvent, and mixed in a ratio of 5.2 to 8.5 parts by weight of green tea extract, 1.2 to 4.5 parts by weight of maekmundong extract, 2.0 to 3.5 parts by weight of sedge extract, and formulation A cosmetic composition for improving skin wrinkles, characterized in that it has excellent stability.
delete According to claim 1,
A cosmetic composition for improving skin wrinkles, characterized in that it is also for skin aging prevention.
According to claim 1,
The composition is
A cosmetic composition for improving skin wrinkles, characterized in that it has any one formulation selected from the group consisting of lotions, emulsions, creams, foams, gels, nourishing lotions, softening lotions, packs, softening water, emulsions, essences, ampoules, and suspensions.

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