KR100444196B1 - Cosmetic composition containing medical plant complex extracts stabilized with nanoliposome - Google Patents

Abstract

The present invention is stabilized with nanoliposomes, characterized in that it contains 0.01 ~ 98.0% by weight relative to the total weight of the cosmetic composition of the nanoliposomes comprising a composite extract of medicinal plants consisting of Echochocho, xylose, marjoram, alponia and three hundred skin The present invention relates to a cosmetic composition containing the medicinal plant complex extract containing the medicinal plant, which is excellent in stability and improves sedimentation and discoloration even when the medicinal plant complex extract is contained in an excessive amount, and has a high skin permeability. It is possible to obtain a cosmetic composition containing a medicinal plant composite extract stabilized with nanoliposomes, which is excellent in both anti-aging and whitening effects, in particular, a transparent or liquid cosmetic composition.

Description

Cosmetic composition containing medicinal plant complex extract stabilized with nanoliposomes {COSMETIC COMPOSITION CONTAINING MEDICAL PLANT COMPLEX EXTRACTS STABILIZED WITH NANOLIPOSOME}

The present invention relates to an anti-aging and whitening cosmetic composition containing a composite extract of a medicinal plant consisting of fish vinegar, xylose, marjoram, alponia and three hundred skin stabilized with nanoliposomes.

Liposomes are generally made of phospholipids. These phospholipids consist of hydrophobic long hydrocarbon chains and hydrophilic polar groups, and the hydrophobic long hydrocarbons consist of sphingosin derivatives or glycerol derivatives. The other side has ester linkages with choline, ethanol amine, serine, inositol or glycerol. Phospholipids form these regularly arranged structures in the water medium, namely micelles or lamellar structures.

Liposomes are also similar in structure to biological membranes and are being studied as membrane model systems and physiological mediators (R. Sundler. D. Papahadjpovlos, Biochim. Biophys. Acta, 648.743 (1981)). It is mainly used in emulsion-based products (patent application No. 92-2287), such as lotions and creams, in order to facilitate the delivery to the skin by collecting the ingredients to further increase the effect of these active ingredients.

Xylose (cotton; Gossypium aboreum Var. Indicum LAN, Xylobios Syrup ) is an annual herb that belongs to the Malvaceae and is also known as cotton. It is effective for urination, lobster, hemostatic action, and has skin moisturizing ability by having similar ingredient to skin natural moisturizing factor (NMF).

Houttuynia cordata (Houttuynia cordata Tunb) is a saururaceae, and shoot the shadows of the mountains for many years been the country, including the southern provinces of Jeju and Ulleungdo grow on dry land, many years the pool. As a pharmacological action, there is an antibacterial effect, inhibits the development of Staphylococcus aureus, hemolytic streptococci, etc., has an effect of enhancing immune function, anti-inflammatory action, diuretic action, antitussive action, chronic bronchitis. As cosmetics, sebum secretion, antibacterial, deodorant, and inflammation inhibitory effects are known (Anduk Kyun, Korea Herbal Pension, P110, 1998. 1). In addition, it promotes the regeneration of skin hard tissues to remove skin diseases and wrinkles on the face (Patent Application No. 98-838182), prevents and treats blemishes and skin diseases, and prevents aging (Patent Application 2000- 81443).

Alpinia speciosa (wendl.) K. Schum is a plant in the family Ginger. Efficacy is known to have antibacterial, anti-inflammatory, moisturizing and astringent effects, and has been applied to cosmetics for promoting collagen synthesis, free radical scavenging, estrogen-like activity, preventing hair loss and treating cosmetics (Patent Application 2000-7004019).

Marjoram ( Origanum Majorana ) is a perennial plant of the family Lamiaceae and has been known to have the strongest fragrance among herbs since ancient times. It has antibacterial and antifungal action (Patent Application No. 98-21257), and is a natural herb substance used as a fragrance (Patent Application No. 99-9857) as a natural ingredient harmless to the human body having excellent antibacterial and bactericidal action. It has a sweet and fragrant aroma and is widely used as an antidote and widely used as a food spice and bath. In addition, it relaxes the nervous system to relieve anxiety and stress, is effective for muscle pain and is known to be good for back pain and stomach cramps, indigestion, constipation.

Morus alba. Is the root bark of the mulberry tree, the mulberry, the mulberry, and the crayfish. It contains various kinds of flavonoids. It is effective for antitussive and diuretic effect, anti-allergic effect, fever, headache, and itching of skin. When used in cosmetics, it is known to have a whitening effect on skin blemishes and freckles (Patent Application No. 97-33567).

In general, plant extracts having such efficacy and effect have many problems in terms of stability when used at higher concentrations than cosmetics or pharmaceuticals. Therefore, in particular, the use of liquid products and transparent products such as skin lotions has been extremely limited.

Specifically, excessive use of such plant extracts in the product often results in precipitation, since the plant extracts bind together with weakly charged substances in the formulation and precipitate together. In addition, when the plant extract is used in a transparent product, the plant extract may be easily oxidized by physical factors such as external ultraviolet rays, thereby changing its own color itself, thereby causing a color change of a transparent product such as a skin lotion. Problems have arisen.

As a solution to this problem, there is a method of adding a small amount of the plant extract raw material to the above products, but there is a problem that it is difficult to expect the efficacy because the use of a small amount of the plant extract. In addition, there is a method of encapsulating granules using phospholipids, but in the case of a transparent liquid product, there is a problem in that the plant extract cannot be suspended because of no viscosity.

An object of the present invention, to solve the problems that occur when using the medicinal plant in a transparent product or liquid product, such as skin lotion, very high stability, high skin permeability and excellent efficacy and effect of the medicinal plant, nano liposomes To provide a stabilized medicinal plant composite extract-containing cosmetic composition.

1 is a graph showing the size of the liposomes according to the number of passes of the high pressure emulsifier.

In order to achieve the above object, according to the present invention, containing 0.01 ~ 98.0% by weight of the nanoliposome comprising a composite extract of medicinal plants consisting of Eoseongcho, xylose, marjoram, alponia and three hundred skin Provided is a medicinal plant composite extract-containing cosmetic composition stabilized with nanoliposomes.

The present invention is described in detail as follows.

In the present invention, the medicinal plant complex extract is obtained by using together Eoseongcho, xylose, marjoram, alponia and three hundred skin, the method of producing such a medicinal plant complex extract is as follows.

Echo herb, xylose, marjoram, alponia and lettuce are mixed and an extractant is added thereto.

These are preferably mixed in proportions of 10 to 20% by weight, 10 to 20% by weight, 10 to 20% by weight, 20 to 30% by weight, and 20 to 40% by weight, respectively. The whitening and anti-aging effects will be evenly distributed, as well as the maximum synergistic effect.

As the extraction solvent of the medicinal plants, alcohols such as methanol, isopropyl alcohol, propylene glycol, ethanol, purified water, and the like may be used. Among them, it is preferable to use 50% 1,3-butylene glycol aqueous solution which has an antiseptic effect and can minimize the loss of vegetable polysaccharides.

Subsequently, it is heated for 4 to 6 hours at 50 to 60 ° C., and then extracted and filtered under ultra-high frequency decompression of a 40 kHz frequency range while heating at 80 to 90 ° C. for 6 to 8 hours to obtain each extract.

This is concentrated again to a concentration of 20 to 30% solids at a pressure of 600 ~ 900mmHg in a vacuum concentrated flask, and then subjected to far-infrared treatment.

In the far-infrared treatment, it is preferable to inject far-infrared light after leaving it for 24 hours in an elvan container known to emit far infrared rays having a wavelength of 10 ⁴ to 10 ⁶ nm.

In this way, by undergoing a far-infrared treatment process, such as aged in a far-infrared radiated gangue stone container, it increases the activity of the active ingredient and has excellent effects on the stability of preservatives and raw materials and the skin safety (the skin irritation effect due to high skin affinity). Can be obtained.

As described above, precipitation of medicinal plant extracts in transparent or liquid products may occur. In particular, in the case of obtaining an extract using a combination of the five medicinal plants as described above, the properties are dark brown, highly concentrated phase, unlike the existing plant extract, and the amount of the active ingredient is higher than the existing plant extract. Therefore, when it is added to a cosmetic product in large quantities, the problem of product stability is large. In particular, in the case of transparent liquid products such as skin lotions, they easily react with other weak ionic cosmetic ingredients to easily precipitate and precipitate.

Therefore, according to the present invention, such a medicinal plant complex extract is contained in the nanoliposomes in order to have excellent efficacy and stability and excellent skin permeability in a transparent product or a liquid product.

Such nanoliposomes are made to have a small size by passing a liposome consisting of phospholipid, glutathione, polysorbate 20, and ethanol seven times through a high pressure emulsifier.

The medicinal plant complex extract is added in an amount of 20 to 40 wt% based on the total weight of the medicinal plant complex extract containing nanoliposomes. When added below 20% by weight, the effect is hard to expect, and when added above 40% by weight, no marked increase in efficacy effect is seen.

The obtained nanoliposomes stabilized medicinal plant complex extract can be added to anti-aging and whitening cosmetics such as nutrition lotion, nutrition cream, essence, etc., in particular can be added to liquid or transparent cosmetics. The nanoliposomes stabilized with the medicinal plant complex extract are contained in an amount of 0.01-98.0 wt% based on the total weight of the composition such as skin. If it is less than 0.01% by weight, it is difficult to expect an effective effect, and if it is more than 98.0% by weight, there is no obvious increase in efficacy. 0.1-50 weight% is preferable, and 10-30 weight% is more preferable.

The following Examples, Comparative Examples, Preparation Examples, and Test Examples are provided to explain the present invention in more detail, and the scope of the present invention is not limited thereto.

(Example 1)

Preparation of Medicinal Plant Complex Extract

15 g of dried xylose, 15 g of edible vinegar, 25 g of alpinia, 15 g of marjoram, and 30 g of lettuce were dried until mechanically unchanged. Crushed 600 g of 50% 1,3-butylene glycol was added to 100 g, and then heated at 50 ° C. for 4 hours, and then extracted under ultra-high pressure reduction in a 40 kHz frequency region while heating for 6 hours at a high temperature of 85 ° C., and 400 mesh Filtered through a sieve. This was concentrated again to a concentration of about 30% solids at a pressure of 600 ~ 900mmHg in a concentrated flask under reduced pressure, and left for 24 hours in an ganban stone vessel to prepare a medicinal plant complex extract.

(Example 2)

Preparation of Nanoliposomes with Stabilized Medicinal Plant Complex Extracts

Phospholipids, glutathione, 95% modified ethanol, glycerin, and polysorbate 20 were emulsified under high pressure to form a closed bilayer nanoliposome. That is, the phase A of the following Table 1 was heated up to 95 degreeC, and it mixed and melted uniformly. It was cooled to 40 ° C and phase B was mixed. This was passed through a high pressure emulsifier (Microfludizer M210EH, Microfluidies, USA) seven times under a pressure of 1200 bar and a flow rate of 500 m / s to obtain a nanoliposome stabilized with the medicinal plant complex extract.

The size of the liposomes according to the number of passes through the high pressure emulsifier is shown in FIG. 1. The size of the liposome obtained through one pass was about 133 nm, but the size of the seventh was about 75 nm.

(Comparative Example 1)

Preparation of Nanoliposomes

Nanoliposomes were prepared in the same manner as in Example 2 except that the medicinal plant complex extract was not added.

(Production Example 1 and Comparative Production Examples 1 to 3)

In Table 2, after completely dissolving phase B and phase C, respectively, phase B was added to phase C and stirred at 3000 rpm or more. Subsequently, phase A was added thereto and stirred at 3000 rpm or more. Example A was a case in which the nanoliposomes stabilized with the medicinal plant complex extract of Example 2 were prepared as Preparation Example 1, and each of the medicinal plant complex extracts of Example 1 and the nanoliposomes of Comparative Example 1 were mixed. Comparative Example 2 was used for the medicinal plant complex extract of Example 1, and Comparative Example 3 was used for the case where there was no A phase.

(Test Example 1)

Skin moisturizing effect

Forty healthy females (mean age 29.5 years) were divided into four groups of 10 healthy women at 22 ~ 24 ℃, 55% relative humidity and no air flow. Preparation Examples 1 and Comparative Preparation Examples 1 to 3 were applied twice a day for 6 weeks to the entire face centered around the eyes in each group. By using Skicon-200 (IBS Co. Japan), the moisture content of the cheek area was measured before and after application to measure the persistence of the moisturizing effect. The results are shown in Table 3 as an average value for each test group.

Skin moisture (unit: electric conductivity μΩ) Before the test 6 weeks after application % Improvement Example 1 630 ± 10 890 ± 10 41.27 Comparative Production Example 1 625 ± 10 685 ± 10 9.60 Comparative Production Example 2 630 ± 10 675 ± 10 7.14 Comparative Production Example 3 630 ± 10 650 ± 10 3.17

Improvement = [(6 weeks after application-before test) / before test] x 100

According to Table 3, the cosmetic composition containing the nanoliposomes stabilized medicinal plant complex extract according to the present invention (Preparation Example 1) is 31.67%, 34.13% compared to Comparative Preparation Example 2, the skin moisture content, 38.1% improvement compared to Comparative Preparation Example 3. As a result, the nanoliposome of the present invention is smaller than the general emulsified particles, so that it is easy to penetrate the skin and increase the persistence of the skin moisturizing effect.

(Test Example 2)

Skin whitening effect

To find out the effect of cosmetics containing nanoliposomes stabilized with xylose, eochochocho, alponia, marjoram, and cephalophyte complex extracts, and the effect as an external skin agent on freckles, a double control method (Well Control Test) Was carried out. About this result, L value (brightness) was computed using the color difference meter (Chromameter CR-300 by the Minolta company).

As a test method, 40 healthy women (average age 29.5 years old) were divided into 4 groups of 10 people each, and the upper arm was irradiated with ultraviolet rays 0.88J / ㎠, 3 times a day. In each group, the samples according to Production Examples 1 and Comparative Production Examples 1 to 3 were applied twice / day for 30 days, and the ΔL value which is the difference between the L value before application and the L value over time after application was given by the following equation. Obtained according to the comparison of the disappearance rate of pigmentation.

The results are shown in Table 4.

Whitening Effect L (%) Preparation Example 1 81.0 Comparative Production Example 1 65.0 Comparative Production Example 2 63.0 Comparative Production Example 3 6.0

In Table 4, when using Preparation Example 1 containing a nano liposome stabilized xylose, eoseongcho, Alpinia, marjoram, baekbaekpi complex extract increased by 16% compared to Comparative Preparation Example 1, 18% compared to Comparative Preparation Example 2 did. Therefore, it can be seen that the cosmetic containing nanoliposomes stabilized medicinal plant complex extract according to the present invention not only prevents blackening of the skin, but also removes skin stains, leading to an excellent whitening synergistic effect.

(Test Example 3)

Skin elasticity effect

40 healthy women (average age 29.5 years) over 20 years old were divided into 4 groups under the conditions of 22 ~ 24 ℃ and 55% relative humidity. In Comparative Preparation Example 2 and Comparative Preparation Example 3 in Group D were formulated according to Table 2, respectively, and applied for 12 weeks (2 times / day) around the eyes, and then, the skin elasticity measuring instrument (Cutometer SEM 575, C + K). Electronic Co., Germany) was used to measure skin elasticity. The test results are described in Table 5 below as ΔR5 values (R5 (12 weeks) -R5 (0 weeks)), which are average values for each test group of the Cutometer SEM 575. The closer it is, the better the elasticity is.

In addition, the improvement degree was computed by the following formula.

Improvement (%) = (△ R5 / Before Application) X 100

Skin elasticity test result (12 weeks) Experimental product Before application After application Skin elasticity enhancing effect (△ R5) % Improvement Preparation Example 1 0.74 0.94 0.20 27.03 Comparative Production Example 1 0.71 0.83 0.12 16.90 Comparative Production Example 2 0.71 0.81 0.10 14.08 Comparative Production Example 3 0.73 0.75 0.02 2.74

In Table 5, when using Preparation Example 1 containing the nanoliposome stabilized xylose, Echoseong, Alpineia, marjoram, baekbaekpi complex extract 10.13% compared to Comparative Preparation Example 1, compared to Comparative Preparation Example 2 It increased by 12.95% and 24.29% compared with Comparative Production Example 3. Therefore, it can be seen that the cosmetic containing nanoliposomes stabilized medicinal plant complex extract according to the present invention effectively enhances skin elasticity.

(Test Example 4)

Skin penetration effect

DE (Dermal Equivalent) 1x10 ⁵ cells / ml of normal human fibroblasts, 3mg / ml collagen aqueous solution: 5X DMEM: 2.2% sodium bicarbonate and 200mM Hepes buffer in collagen gel structure similar to the fibrous tissues Inoculated in a medium mixed with 7: 2: 1 of 0.05 N sodium hydroxide, and incubated for 7 days at 5% CO ₂ , 37 ° C.) with a membrane made of 3 μm porous polycarbonate. Inoculate 1 × 10 ⁵ cells / ml of epidermal Keratinocytes on the surface of the DE where the fibroblasts were cultured, and put K-SFM medium containing EGF and BPE inside and outside. Incubated for 7 days. Subsequently, the medium inside the plate was removed to allow air to come into contact with the surface of the cultured cells, followed by incubating for 2 weeks in a medium in which K-SFM containing 10% FBS and DMEM without EGF were mixed in the same amount. , Artificial skin with multilayer epidermis and dermis was obtained. Here, Preparation Example 1 and Comparative Preparation Examples 1 to 3 of the present invention were applied to the artificial skin tissue of the top layer. After 4 hours of incubation, the amount of the complex extract that penetrated the epidermis and dermis of the artificial skin in the same amount of K-SFM containing 10% FBS and DMEM without EGF was analyzed by HPLC. The test results are shown in Table 6 below.

Penetration amount of complex extract (㎍ / ㎖) Preparation Example 1 7.2 Comparative Production Example 1 0.9 Comparative Production Example 2 0.9 Comparative Production Example 3 0.0

In Table 6, the cosmetic containing the nanoliposome stabilized medicinal plant complex extract of the present invention (Preparation Example 1) is about 8 times as compared to Comparative Preparation Example 1 and Comparative Preparation Example 2 using the medicinal plant complex extract as it is It can be seen that the effect is excellent.

(Test Example 5)

Stability experiment

The stability of the formulations for the cosmetics containing nanoliposomes stabilized xylose, eoseongcho, alponia, marjoram, baekbaekpi complex extract according to the present invention was measured by the following method.

Samples stored in an opaque vitreous container in a constant temperature bath maintained at 45 ° C. in Preparation Example 1 and Comparative Preparation Examples 1 to 3 were stored in a opaque glass container in a completely shaded refrigerator kept at 4 ° C. for 12 weeks. The samples stored for 12 weeks, and samples stored for 12 weeks in a circulation chamber (once / day) circulating at 37 ° C. at −5 ° C. were compared and measured for the degree of discoloration and precipitation. The results of the evaluation of the product separation and the degree of discoloration classified into the following six grades are shown in Table 7 below.

Product discoloration evaluation criteria:

0: No change 1: Very little separation (discoloration, precipitation)

2: A little separation (discoloration, precipitation) 3: A little severe separation (discoloration, precipitation)

4: severely separated (discolored, precipitated) 5: extremely severely separated (discolored, precipitated)

Temperature Separation and discoloration degree of test substance Preparation Example 1 Comparative Production Example 1 Comparative Production Example 2 Comparative Production Example 3 45 ℃ 0 One One 0 4 ℃ 0 One One 0 cycle 0 One One 0

Temperature Precipitation degree of test substance Preparation Example 1 Comparative Production Example 1 Comparative Production Example 2 Comparative Production Example 3 45 ℃ 0 3 2 0 4 ℃ 0 5 5 0 cycle 0 4 5 0

Through Table 7, Preparation Example 1 and Comparative Preparation Example 3 stabilized through nanoliposomes were stable without discoloration or separation symptoms at 4 ℃, 45 ℃ and circulating test, but without directly stabilizing the medicinal plant composite extract solution In Comparative Preparation Examples 1 and 2 used, slight discoloration occurred at 4 ° C., 45 ° C., and circulation tests, indicating that the complex extract was unstable.

In addition, in Table 8 for the degree of precipitation, Comparative Preparation Examples 1 and 2 were severely precipitated at 4 ℃ and circulation, while Example 1 did not occur precipitation at all, medicinal plant complex extract stabilized with nanoliposomes according to the present invention It was found that it was stabilized.

As such, when an excess (10% by weight) is added to the clear liquid formulation without stabilizing the medicinal plant complex extract, the complex extract easily reacts with other weak ionic polymers in the formulation, causing precipitation and discoloration. However, when the medicinal plant complex extract is stabilized with nanoliposomes and applied to a transparent liquid formulation as in the present invention, it can be seen that precipitation and discoloration reaction are minimized even when the medicinal plant complex extract is added in excess.

(Manufacture example 2)

Lotion (skin lotion)

Ingredient Parts by weight Nanoliposomes with Stabilized Medicinal Plant Complex Extracts 35.0 1.3-butylene glycol 5.0 Allantoin 0.1 1% hyaluronic acid 1.0 glycerin 5.0 Polyoxyethylene Cured Castor Oil 0.5 Polyoxyethylene Hydrogenated Castor Oil 0.5 95% denatured ethanol 10.0 Benzophenone-9 0.05 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100

(Manufacture example 3)

Concentrated Cosmetic Water (Essence: Solubilization Type)

Ingredient weight% Nanoliposomes with Stabilized Medicinal Plant Complex Extracts 35.0 1.3-butylene glycol 2.0 95% denatured ethanol 10.0 Triethanolamine 0.2 Polyacrylamide 0.5 Allantoin 0.1 Panthenol 0.1 1% hyaluronic acid 3.0 Seaweed Extract 1.0 Carboxyvinyl Polymer 0.2 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100

(Manufacture example 4)

pack

Ingredient Parts by weight Nanoliposomes with Stabilized Medicinal Plant Complex Extracts 1.0 1.3-butylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.2 95% denatured ethanol 7.0 Polyvinyl alcohol 15.0 Cellulose gum 0.2 PEG-40 hydrogenated castor oil 0.8 Triethanolamine 0.3 Allantoin 0.1 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100

According to the present invention, it is excellent in stability and precipitation and discoloration are improved even when the medicinal plant complex extract is contained in an excessive amount. Excellent, nanoliposome stabilized medicinal plant complex extract-containing cosmetic compositions, in particular transparent or liquid cosmetic compositions can be obtained.

Claims (4)

Nanoliposomes stabilized with nanoliposomes, characterized in that it comprises 0.01 ~ 98.0% by weight relative to the total weight of the cosmetic composition of the nanoliposome comprising a composite extract of medicinal plants consisting of Eoseongcho, xylose, marjoram, alponia and three hundred skin Complex extract containing cosmetic composition. The method of claim 1, wherein the nanoliposomes are obtained by passing a liposome consisting of phospholipid, glutathione, polysorbate 20, ethanol seven times through a high-pressure emulsifier, nano-liposomes stabilized medicinal plant-containing cosmetics containing cosmetics Composition. According to claim 1, wherein the complex extract of the medicinal plant is characterized in that it contains 20 to 40% by weight relative to the medicinal plant complex extract containing nanoliposomes, nano-liposomes stabilized medicinal plant complex extract containing cosmetic composition. The medicinal plant according to any one of claims 1 to 3, wherein the medicinal plants are 10-20% by weight, 10-20% by weight of xylose, 10-20% by weight of marjoram, 20-30% by weight of alpinia and 20 Cosmetic composition containing a medicinal plant composite extract stabilized with nano-liposomes, characterized in that consisting of ~ 40% by weight.