KR100512330B1 - Cosmetic composition containing baobob extracts stabilized with nanoliposome - Google Patents

Abstract

The present invention relates to a nanoliposome stabilized baobab extract containing cosmetic composition, characterized in that it contains 0.1 to 20% by weight, based on the total weight of the cosmetic composition of the nanoliposome comprising the baobab extract, the baobab extract nano By containing in liposome, the cosmetic composition which is excellent in the moisture retention by Baobab tree extract, and excellent in stability and skin permeability can be obtained.

Description

Skin moisturizing cosmetic composition containing baobab extract stabilized with nanoliposomes {COSMETIC COMPOSITION CONTAINING BAOBOB EXTRACTS STABILIZED WITH NANOLIPOSOME}

The present invention relates to a skin moisturizing and elastic cosmetic composition containing baobab extract stabilized with nanoliposomes.

Liposomes are generally made of phospholipids. These phospholipids consist of hydrophobic long hydrocarbon chains and hydrophilic polar groups, and the hydrophobic long hydrocarbons consist of sphingosin derivatives or glycerol derivatives. The other side has ester linkages with choline, ethanol amine, serine, inositol or glycerol. Phospholipids form these regularly arranged structures in the water medium, namely micelles or lamellar structures.

Liposomes are also similar in structure to biological membranes and are being studied as membrane model systems and physiological mediators (R. Sundler. D. Papahadjpovlos, Biochim. Biophys. Acta, 648.743 (1981)). It is mainly used in emulsion-based products (patent application No. 92-2287), such as lotions and creams, in order to facilitate the delivery to the skin by collecting the ingredients to further increase the effect of these active ingredients.

Baobab tree (Baobab, Adansonia digitata) is a deciduous tree with a spring box. It is 20 m high, 10 m circumferential height, 10 m long, and branches are one of the largest trees in the world. It is considered one of the most sacred trees in Africa, and it can inhabit people and bury their bodies. It is also called 'dead rat tree' because its shape looks like a rat. The leaves are 5-7 small leaves, ileum. The flower is white with 5 petals about 15 cm in diameter. Fruits are like scrubbers, 20-30 cm long, hairy, hard, with long fruiting. Bark (樹皮) is a fiber, leaves and branches are used as feed, and the fruit is used for food. It is said to be 5,000 years old and grows in tropical Africa.

In addition, as the tree grows older as the trunk grows in rain, the natives used it as an emergency drinking water for hunting, and as the water content was 75%, it became a hideout as well as a food garden for animals in the dry season.

The tree also contains a number of active ingredients that function to sustain life on dry land.

The benefits of these baobabs are moisturizing, epidermal layering and protection, and skin luster enhancement (Adesanya SA, Idowu TB and Elujoba AA (1988), Antisickling activity of Adansonia Digitata. Planta Medica 54 (4): 374; Andrianaivo-Rafehivola AA , Siess MH and Gaydou EM (1995) Modifications of hepatic drug metabolizing enzyme activities in rats fed baobab seed oil containing cyclopropenoid fatty acids.Food and Chemical Toxicology, 33 (5): 377-382).

Such extracts of baobab have many problems in terms of stability when used in cosmetics as a moisturizing raw material. In particular, its use is limited to transparent products such as skin lotions or liquid products.

Specifically, excessive use of such baobab extract in the product may result in precipitation, since the baobab extract is combined with the weakly charged substances in the formulation and precipitates together. In addition, when the baobab extract is used in a transparent product, the baobab extract may be easily oxidized by physical factors such as external ultraviolet rays to change its own color itself, thereby changing the color of the transparent product such as skin lotion. There was a problem that caused it.

As a solution to this problem, although a small amount of baobab tree extract raw material can be added to the above products, there is a problem in that it is difficult to expect the efficacy because only a small amount of baobab tree extract can be used. In addition, there is a granule encapsulation method using phospholipids, but in the case of a transparent liquid product, there is a problem in that the baobab extract cannot be suspended because it has no viscosity.

An object of the present invention, to solve the problems caused when using the extract of the baobabs in a transparent product or a liquid product, such as skin lotion, very high stability and high skin permeability, but also excellent efficacy and effect of the extract, It is to provide a baobab tree extract-containing cosmetic composition stabilized with nanoliposomes.

In order to achieve the above object, according to the present invention, characterized in that the nano liposome containing a baobab tree extract containing 0.1 to 20% by weight relative to the total weight of the cosmetic composition, nano liposome stabilized baobab tree extract containing cosmetic composition This is provided.

The present invention is described in detail as follows.

The extract of the baobab can be obtained by using various parts of the baobab, but it is excellent in terms of the effect of obtaining the extract of the baobab.

As the extraction solvent of the baobab, alcohols such as methanol, isopropyl alcohol, propylene glycol, ethanol, purified water, and the like can be used. Among them, it is preferable to use 50% 1,3-butylene glycol aqueous solution and stable purified water which have an antiseptic effect and can minimize the loss of vegetable polysaccharides.

When purified water is used as the extraction solvent, the extract is preferably obtained by hot water extraction. More specifically, baobabs are dried and ground until there is no change in weight. A solvent of the same weight is added to the obtained powder, followed by agitation at 50 to 70 ° C. for 40 to 50 hours, followed by filtration to obtain an extract.

Nanoliposomes are made of a liposome consisting of phospholipid, glutathione, polysorbate 20, and ethanol seven times through a high pressure emulsifier to reduce the size.

The baobab extract is added in an amount of 10 to 40 wt% based on the total weight of the extract-containing nanoliposomes. When added in less than 10% by weight, the effect is hard to expect, even when added in excess of 40% by weight does not show a marked increase in efficacy effect.

The nanoliposomes obtained by stabilizing the obtained baobab extract can be added to moisturizing cosmetics such as nourishing lotion, nourishing cream, and essence, and in particular can be added to liquid or transparent cosmetics.

Nanoliposome stabilized Baobab tree extract is contained in 0.1 to 20% by weight based on the total weight of the composition. If it is less than 0.1% by weight, it is difficult to expect an effective effect, and if it exceeds 20% by weight, there is no obvious increase in efficacy. Therefore, 0.1-20 weight% is preferable and 0.1-10 weight% is more preferable.

The following Examples, Comparative Examples, Preparation Examples, and Test Examples are provided to explain the present invention in more detail, and the scope of the present invention is not limited thereto.

(Example 1)

Preparation of Baobab Extract

The leaves of the baobabs were dried at room temperature until there was no change in weight. It was ground by pulverization to powder. 50 g of water was added to 50 g of baobab leaf dry powder, followed by bathing at about 60 ° C. for about 48 hours. The extract removed from the debris was first filtered using a 300 mesh filter paper, and the filtered extract was filtered again and again with 0.40 μm filter paper to obtain an extract.

(Test Example 1)

Skin moisturizing effect

In order to measure the skin moisturizing effect of simple raw materials before nanoliposomalization, Na-PCA (Sodium Pyrolidone Carboxylic acid), Chondroitin, which is a component of NMF (Natural Moisturizing Factor) as a comparison target with Baobab tree extract of Example 1 Na-Condroitin Sulfate and Na-hyaluronate 1% aqueous solution was measured to reduce the epidermal moisture loss (TEWL) after barrier damage, a standard measure of skin barrier function, respectively.

The specific method is as follows.

Acetone was instilled in the back of 8-12 week old hairless mice to damage the skin barrier. When transepidermal water loss (TEWL) reached 4.0 g / m ² / h, the sample was applied to an area of 5 cm ² . TEWL was measured with Tewameter, an evaporimeter from CK (Courage + Khazaka, Cologne, Germany). After 6 hours of application, TEWL was measured to evaluate the extent to which TEWL was reduced to evaluate the extent to which skin barrier function was restored.

The recovery rate used for the efficacy evaluation was calculated as in Equation 1 below.

(Bt = 6: TEWL measured value 6 hours after skin barrier injury)

     Bt = 0: TEWL measurement before skin barrier injury

     Bt = d: TEWL measured immediately after skin barrier damage)

The results are shown in Table 1 (unit: mg / m 2 / hr).

As a result of TEWL measurement, Baobab tree extract used in the present invention was 26.8% compared to Na-PCA (Sodium Pyrolidone Carboxylic acid), 25.13% compared to Na-Condroitin Sulfate, and Na-Hyaluronate. 28.07% showed a good effect.

(Example 2)

Preparation of Nanoliposomes with Stabilized Baobab Tree Extract

Phospholipids, glutathione, 95% modified ethanol, glycerin, and polysorbate 20 were emulsified under high pressure to form a closed bilayer nanoliposome. Phase A in Table 2 was heated to 95 ° C., uniformly mixed and dissolved. It was cooled to 40 ° C and phase B was mixed. This was passed through a high pressure emulsifier (Microfludizer M210EH, Microfluidies, USA) seven times under a pressure of 1200 bar and a flow rate of 500 m / s to obtain a nanoliposome stabilized Baobab tree extract.

The size of the liposome obtained through one pass was about 133 nm, but the size of the seventh was about 75 nm.

Raw material name Content (% by weight) A Phospholipids 4 Glutathione 0.1 95% modified ethanol 20 glycerin 30 Polysorbate 20 4 B Baobab Extract 20 Purified water Remaining amount system 100

(Comparative Example 1)

Preparation of Nanoliposomes

Nanoliposomes were prepared in the same manner as in Example 2 except that the baobab extract was not added.

(Manufacture example 1)

Preparation of Cosmetics Containing Nanoliposomes with Stabilized Baobab Tree Extract

Using the following starting materials, an emulsion was prepared according to a conventional method for producing a cosmetic.

Raw material name Content (unit: weight%) Polysorbate-60 1.5 Sorbitan stearaytan 0.5 Stearic acid One Squalane 5 Cetostearyl alcohol 2 Cetyl octanoate 5 Carbomer 0.15 Triethanol amine 0.2 glycerin 5 Nanoliposomes stabilized Baobab extract of Example 2 0.1 incense A reasonable amount antiseptic A reasonable amount Purified water Remaining amount total 100

(Production Examples 2 to 5)

An emulsion was prepared in the same manner as in Preparation Example 1, except that 1, 5, 10, and 20 wt% of the nanoliposomes of the Baobab tree extract of Example 2 were stabilized, respectively, to Preparation Examples 2 to 5.

(Comparative Production Examples 1 and 2)

The emulsion was prepared in the same manner as in Preparation Example 1, except that 0.05 and 25% by weight of the nanoliposomes, which stabilized the baobab extract of Example 2, were added, respectively, to Comparative Preparation Examples 1 and 2.

(Test Example 2)

Skin moisture measurement

Eighty healthy females (mean age 29.5 years) were divided into eight groups of 22 healthy women (22 ~ 24 ℃, 55% relative humidity, no air flow). The cosmetics of Preparation Examples 1 to 6 and Comparative Preparation Examples 1 to 2 were applied to the entire face twice a day for 6 weeks, mainly around the eyes of each group. By using Skicon-200 (IBS Co. Japan), the moisture content of the cheek area was measured before and after application to measure the persistence of the moisturizing effect. The results are shown in Table 4 as an average value for each test group.

The improvement was calculated according to the following equation.

Before the test 6 weeks after application Improvement (%) Preparation Example 1 630 ± 10 650 ± 10 3.17 Preparation Example 2 625 ± 10 685 ± 10 9.6 Preparation Example 3 630 ± 10 890 ± 10 41.27 Preparation Example 4 630 ± 10 675 ± 10 7.14 Preparation Example 5 630 ± 10 650 ± 10 3.17 Comparative Example 1 630 ± 10 635 ± 10 0.78 Comparative Example 2 630 ± 10 630 ± 10 0

According to the table, it is preferable to contain 0.1 to 20% by weight of the nano liposome stabilized Baobab tree extract in the cosmetic.

(Comparative Production Examples 3 to 5)

Emulsion in the same manner as in Preparation Example 1, except that 5% by weight of Na-PCA, sodium chondroitin sulfate, and Na-hyaluronate were used instead of the nanoliposomes stabilized with the baobab extract of Example 2. To prepare, and each of Comparative Preparation Examples 3 to 5.

(Comparative Production Example 6)

Instead of using the nanoliposomes stabilized Baobab tree extract of Example 2, except that 1% by weight of the baobab tree extract of Example 1 and 4% by weight of nanoliposome of Comparative Example 1 is used, The emulsion was prepared by the method.

(Comparative Production Example 7)

Instead of using the nanoliposome stabilized Baobab tree extract of Example 2, except that 1% by weight of the Baobab tree extract of Example 1 was used, the emulsion was prepared in the same manner as in Preparation Example 1.

(Test Example 3)

Skin elasticity effect

Sixty healthy women (average age 29.5 years) over 20 years old were divided into 6 groups under the conditions of temperature of 22-24 ° C and 55% relative humidity, and the cosmetic compositions of Preparation Example 3 and Comparative Preparation Examples 3 to 7 were focused on the eyes. After application for 12 weeks (2 times / day), skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germany). The test results are described in Table 5 below as ΔR5 values (R5 (12 weeks) -R5 (0 weeks)), which are average values for each test group of the Cutometer SEM 575. The closer it is, the better the elasticity is.

In addition, the improvement degree was computed by the following formula.

Improvement (%) = (△ R5 / Before Application) X 100

Skin elasticity test result (12 weeks) Before application After application Skin elasticity enhancing effect (△ R5) % Improvement Preparation Example 3 0.74 0.96 0.22 29.73 Comparative Production Example 3 0.71 0.80 0.09 12.68 Comparative Production Example 4 0.71 0.81 0.1 14.08 Comparative Production Example 5 0.73 0.75 0.02 2.74 Comparative Production Example 6 0.72 0.80 0.08 10.00 Comparative Production Example 7 0.73 0.77 0.04 5.19

According to Table 5, when using Preparation Example 3 containing the nanoliposome stabilized Baobab extract, skin elasticity is 17.05% compared to Comparative Preparation Example 3, 15.65% compared to Comparative Preparation Example 4, Comparative Preparation Example 5 26.99%, 19.73% compared to Comparative Preparation Example 6, 24.54% compared to Comparative Preparation Example 7. Therefore, it can be seen that the cosmetic containing nanoliposomes stabilized Baobab tree extract according to the present invention effectively enhances skin elasticity.

(Test Example 4)

Stability experiment

The stability of the formulation for the cosmetic containing the nanoliposome stabilized Baobab leaf extract according to the present invention was measured by the following method.

Samples stored in an opaque vitreous container in a constant temperature bath at constant temperature maintained at 45 ° C., the extracts of Preparation Example 3, Comparative Preparation Examples 6 and 7, stored for 12 weeks, opaque in a fully shaded refrigerator maintained at 4 ° C. For samples stored in a container for 12 weeks and samples stored for 12 weeks in a circulation chamber (once / day) circulating at -5 ° C. to 37 ° C., separation, degree of discoloration, and precipitation were measured.

The separation and discoloration degree of the product was classified into the following six grades and the evaluation results are shown in Table 6 below.

Product discoloration evaluation criteria:

0: no change 1: very little separation (discoloration)

2: Slightly separated (discolored) 3: Slightly separated (discolored)

4: Extremely separated (discolored) 5: Extremely separated (discolored)

Separation and discoloration degree of test substance Temperature Preparation Example 3 Comparative Production Example 6 Comparative Production Example 7 45 ℃ 0 3 3 4 ℃ 0 2 2 cycle 0 2 3

Through Table 6, Preparation Example 3 stabilized through nanoliposomes was stable without discoloration or separation symptoms at 4 ℃, 45 ℃ and circulating test, Comparative Preparation Example 6 used directly in the formulation without stabilizing Baobab leaf extract And in the case of 7 separation (discoloration) was found to be unstable.

In addition, the results obtained by classifying the degree of precipitation of the product into the following six grades are shown in Table 7 below.

Product discoloration evaluation criteria:

0: no change 1: very slight precipitation

2: little precipitate 3: little precipitation

4: settling heavily 5: settling extremely severe

Precipitation degree of test substance Temperature Preparation Example 3 Comparative Production Example 6 Comparative Production Example 7 45 ℃ 0 3 3 4 ℃ 0 3 2 cycle 0 3 2

Through Table 7, it was found that the baobab extract stabilized with the nanoliposomes according to the present invention was not precipitated and stabilized.

As such, when the baobab extract is added without stabilization, precipitation and discoloration occur easily by reacting with other weak ionic polymers in the formulation. However, when the baobab tree extract is stabilized with nanoliposomes and applied to the formulation as in the present invention, it can be seen that precipitation and discoloration reaction are minimized even when the baobab tree extract is added in excess.

(Manufacture example 6)

Lotion (skin lotion)

Ingredient weight% Nanoliposomes stabilized Baobab tree extract of Example 2 20.0  1.3-butylene glycol 5.0  Allantoin 0.1  1% hyaluronic acid 1.0  glycerin 5.0  Polyoxyethylene Cured Castor Oil 0.5  Polyoxyethylene Hydrogenated Castor Oil 0.5  95% denatured ethanol 10.0  Benzophenone-9 0.05  antiseptic a very small amount  incense a very small amount  Purified water Remaining amount  system 100

(Manufacture example 3)

Concentrated Cosmetic Water (Essence: Solubilization Type)

Ingredient weight%  Nanoliposomes with Stabilized Medicinal Plant Complex Extracts 20.0  1.3-butylene glycol 2.0  95% denatured ethanol 10.0  Triethanolamine 0.2  Polyacrylamide 0.5  Allantoin 0.1  Panthenol 0.1  1% hyaluronic acid 3.0  Seaweed Extract 1.0  Carboxyvinyl Polymer 0.2  antiseptic a very small amount  incense a very small amount  Purified water Remaining amount system 100

According to the present invention, by containing the baobab tree extract in the nanoliposomes, it is possible to obtain a cosmetic composition excellent in moisturization by the baobab tree extract and excellent in stability and skin permeability.

Claims (3)

Baobab tree extract containing cosmetic composition containing the nano liposomes stabilized Baobab tree extract 0.1 to 20% by weight based on the total weight of the cosmetic composition,    The nanoliposomes are obtained by mixing Baobab tree extracts with liposomes consisting of phospholipid, glutathione, polysorbate 20 and ethanol seven times through a high-pressure emulsifier, nanoliposome stabilized baobab tree extract-containing cosmetic composition . delete The method of claim 1, The baobab tree extract is characterized in that it contains 10 to 40% by weight relative to the nanoliposome stabilized Baobab tree extract, baobab tree-containing cosmetic composition stabilized with nanoliposomes.